RLG00000029942

valine--tRNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004429
Physical Location & Seq
Reverse (-)
51769435 .. 51789690
20256 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029942

Sequence Viewer

Length: 2919 bp
ATGATTCTCCAAACCACCTTCTCCGCCCCGTCTCTTCTTTTCTCCTCCTGCTCTCCTCATCGCCTCAGGCTCCATCCTCTTCTCTTCCCCAAACGACGCCGCCGCGCCGTTATGCCCTCACTCTTCACCACTCCTCGTTGCGTTTCAGTTGCCGCTGCTGCCGCCTCCGACAATGGCGTCTTCACTTCTCCGGAGACTGCCAAGTCCTTTGATTTTACTAATGAAGAGAGGATTTACACCTGGTGGGAGTCTCGGGGATATTTCAGACCAAGCTTTGACCGAGGAAGCGATCCTTTTGTGATTTCAATGCCACCTCCAAATGTCACTGGCTCGCTGCACATGGGGCACGCCATGTTCGTCACACTTGAGGACATTATGGTTAGATACCATCGTATGAAGGGAAGGCCAACACTTTGGCTTCCTGGGACTGATCATGCTGGTATAGCCACACAGTTGGTTGTGGAAAGAATGCTTGCATCAGAAGGAATTAAAAGGGTTGACTTGGGTCGAGATGAGTTTGTGAAAAGAGTTTGGGAGTGGAAAGAGAAGTATGGTGGAACTATCACAAATCAGATCAAAAGGCTTGGTGCTTCTTGTGATTGGACAAGGGAGCATTTCACCCTTGATGAACAGCTAAGTCGAGCTGTTGTTGAGGCCTTTGTTAGACTTCATGAAAAAGGCTTAATCTATCAAGGGTCTTACATGGTGAACTGGTCTCCTAGTCTACAGACTGCTGTTTCTGACTTGGAAGTAGAATATCATGAAGAATCCGGTAGTCTTTATTATATTAAGTATCGTGTTGCTGGAGGCTCAAAGACTGACTTTCTGACAATAGCAACAACACGCCCGGAGACTTTATTTGGTGATGTAGCTATTGCTGTGCATCCTGAGGATGATCGATATTCTAAATACATAAATAGAATGGCTATAGTGCCTATGACATATGGTCGCCATGTCCCTATAATCGCTGACAAGCTTGTTGATAAAGAGTTTGGCACTGGTGTGTTAAAGATAAGCCCTGGACATGATCATAATGATTACAACCTTGCAAAAAAGCTTGGTCTTCCAATACTAAATGTTATGAACAAGGATGGGACACTTAACAAGGTTGCGGGACTATACTGTGGCCTTGATCGGTTTGAGGCTCGGAGGAAACTTTGGGCAGATCTGGAGGAGACAGGATTGGCTGTAAAGAAAGAACCACATACTTTAAGAGTTCCCAGATCTCAGCGTGGTGGAGAAGTGATAGAGCCACTAATAAGCAAGCAGTGGTTTGTAACAATGGAGCCTTTAGCTGAGAAAGCCCTACAAGCAGTTGAAAAAGGAGATCTAAAAATTATACCTGAAAGATTTGACAAGATTTATAATCACTGGCTATCAAATATCAAGGATTGGTGCATAAGCAGACAACTGTGGTGGGGACACCGCATACCAGTTTGGTACATTGTGGGTAAAGATTGTGAAGAGGAATATATAGTTGCGAGGAGTTATGATGAAGCTCTTGGTAAAGCTCAAGAAAAGTATGGAAGAGATGCAAAAATATATCAGGATCCAGACGTTCTTGATACTTGGTTTTCAAGTGCACTGTGGCCTTTCAGTACTCTTGGGTGGCCAGATGTGTCAACAGAGGATTTTAGACGGTTTTATCCTACATCAATGCTGGAAACCGGGCATGACATATTATTCTTTTGGGTTGCGAGGATGGTCATGATGGGAATTGAGTTCACCGGGACAGTTCCTTTTTCACACATTTATCTACATGGTCTCATTAGGGATCCAGAGGGGCGAAAGATGTCAAAAACCTTGGGGAATGTAATAGATCCTTTAGATACAATCAAGGAATACGGAACAGATGCTTTACGATTTACACTGGCTCTAGGGACTGCTGGTCAGGACCTTAATTTATCCACCGAGAGGCTGACATCCAATAAGGCCTTCACTAACAAACTATGGAATGCTGGAAAGTTTGTATTGCAGAATTTGCCCAGTCAGAATGATGCTTCAATTTGGGAAAATATACTCTCGTATAAGTTTGACAAAGTGGAGCTTCTAGACATTCTACCATTGCCAGAACGTTGGGTGATCTCAAAACTTCATTTGCTTATTGACTCTGTGACAGCAAGCTATGACAAATTTTTCTTTGGAGATGTAGGAAGAGAGATATACAATTTTTTTTGGGGTGATTTTGCCGATTGGTATATTGAGGCCAGCAAAGCTCGTCTTTTCCAATCTGGTAGCATCTCTGAGGCTTCTGTTGCTCAGGCAGTTCTTTTGTATGTTTTTGAAAACATACTTAAATTGCTACACCCCTTCATGCCATTTGTAACTGAGGAACTCTGGCAGGCTCTGCCCTATCGGAAAGAAGCTCTTATAGCATCTCCATGGCCACTGACTTCCCTTCCAAGGCAAATCAACTCGATTAAAAAGTTTGAAAATTTACAGGCTTTGACTAGAGCCATTCGGAATGCACGAGCAGAATATTCTGTTGAGCCGGTAAAGCGTATATCAGCATCAATAGTCGCAAATGTAGAAGTTACAGAGTACATAATGAAAGAAAAGGTGGTTTTAGCTCTCCTCTCCAGGCTAGATTTGCAAAGCATCCATTTTACAGATTCTCCACCAGGGAATGCTGATCAATCAGTCCACCTTGTTGCTGGTGATGGACTAGAGGCCTATCTTCCTCTTGCTGATATGGTTGATATCACTTCTGAAATCCAACGGCTTTATAAGCGACTTTCCAAAATGCAGACAGAATATGATGGACTTATAGCTCGGCTCAGTTCTCCTAAATTTATAGAGAAAGCTCCTGAGGATATTGTTCGTGGGGTTCGAGAAAAGGCAGAGGAAACAGAAGAGAAGATCACTCTCACCAAAAACCGTTTAGCATTGCTTGAATCGACTGCTGTTGTTACACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 Val_tRNA-synt_C tRNA-synt_1

Protein Analysis

973

Amino Acids

111.01

Weight (kDa)

6.49

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 78 - 636 1e-149 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 100 - 252 1.2e-08 tRNA synthetases class I (M)
tRNA-synt_1_2 PF13603 306 - 365 5.7e-07 Leucyl-tRNA synthetase, editing domain
tRNA-synt_1g PF09334 398 - 478 7.2e-06 tRNA synthetases class I (M)
Anticodon_1 PF08264 691 - 835 1.4e-39 Anticodon-binding domain of tRNA ligase
Val_tRNA-synt_C PF10458 900 - 964 5.4e-13 Valyl tRNA synthetase tRNA binding arm
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1365, 2727
AasI GACNNNNNNGTC 2 cut(s) 176, 202
AccI GTMKAC 1 cut(s) 724
AccII CGCG 1 cut(s) 105
AccIII TCCGGA 1 cut(s) 190
AciI CCGC 7 cut(s) 24, 100, 103, 153, 162, 1112, 1426
AclI AACGTT 1 cut(s) 2074
AclWI GGATC 6 cut(s) 284, 1544, 1557, 1769, 1782, 1814
AcoI YGGCCR 2 cut(s) 1610, 2384
AcsI RAATTY 4 cut(s) 1978, 2132, 2434, 2789
AcyI GRCGYC 2 cut(s) 97, 177
AdeI CACNNNGTG 1 cut(s) 243
AfaI GTAC 3 cut(s) 1442, 1600, 2543
AfiI CCNNNNNNNGG 2 cut(s) 1914, 2403
AgsI TTSAA 7 cut(s) 306, 1319, 1578, 2004, 2285, 2432, 2894
AhdI GACNNNNNGTC 2 cut(s) 945, 1887
AjnI CCWGG 5 cut(s) 239, 421, 1018, 2579, 2620
AjuI GAANNNNNNNTTGG 2 cut(s) 1919, 1951
AleI CACNNNNGTG 1 cut(s) 1001
Alw21I GWGCWC 1 cut(s) 1585
Alw26I GTCTC 7 cut(s) 36, 188, 255, 720, 845, 1169, 1769
Alw44I GTGCAC 1 cut(s) 1581
AlwI GGATC 6 cut(s) 284, 1544, 1557, 1769, 1782, 1814
AlwNI CAGNNNCTG 1 cut(s) 2347
Ama87I CYCGRG 1 cut(s) 252
Aor13HI TCCGGA 1 cut(s) 190
AoxI GGCC 9 cut(s) 404, 654, 1126, 1589, 1610, 1932, 2205, 2384, 2670
ApaLI GTGCAC 1 cut(s) 1581
ApeKI GCWGC 3 cut(s) 155, 158, 334
ApoI RAATTY 4 cut(s) 1978, 2132, 2434, 2789
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 1894
AsuC2I CCSGG 3 cut(s) 848, 1669, 1729
AsuHPI GGTGA 9 cut(s) 118, 610, 718, 875, 1717, 2092, 2192, 2669, 2860
AvaI CYCGRG 1 cut(s) 252
AvaII GGWCC 1 cut(s) 1894
AxyI CCTNAGG 3 cut(s) 65, 888, 2808
BaeGI GKGCMC 2 cut(s) 348, 1585
BaeI ACNNNNGTAYC 2 cut(s) 376, 409
BalI TGGCCA 2 cut(s) 1612, 2386
BamHI GGATCC 2 cut(s) 1549, 1774
BauI CACGAG 1 cut(s) 2469
BbsI GAAGAC 2 cut(s) 172, 1055
Bbv12I GWGCWC 1 cut(s) 1585
BbvI GCAGC 3 cut(s) 142, 145, 321
BccI CCATC 7 cut(s) 81, 396, 1085, 1696, 1705, 2654, 2753
BceAI ACGGC 2 cut(s) 92, 2735
BciT130I CCWGG 5 cut(s) 241, 423, 1020, 2581, 2622
BclI TGATCA 3 cut(s) 430, 1027, 2632
BcnI CCSGG 3 cut(s) 848, 1669, 1729
BcoDI GTCTC 7 cut(s) 36, 188, 255, 720, 845, 1169, 1769
BfaI CTAG 6 cut(s) 720, 1877, 2051, 2451, 2585, 2666
BfmI CTRYAG 2 cut(s) 725, 927
BglII AGATCT 3 cut(s) 1165, 1223, 1327
BisI GCNGC 7 cut(s) 100, 103, 153, 156, 159, 162, 335
BlsI GCNGC 7 cut(s) 101, 104, 154, 157, 160, 163, 336
BmcAI AGTACT 1 cut(s) 1600
Bme1390I CCNGG 8 cut(s) 241, 423, 848, 1020, 1669, 1729, 2581, 2622
Bme18I GGWCC 1 cut(s) 1894
BmeRI GACNNNNNGTC 2 cut(s) 945, 1887
BmeT110I CYCGRG 1 cut(s) 252
BmgT120I GGNCC 1 cut(s) 1894
BmiI GGNNCC 4 cut(s) 71, 1287, 1551, 1776
BmrFI CCNGG 8 cut(s) 241, 423, 848, 1020, 1669, 1729, 2581, 2622
BmrI ACTGGG 1 cut(s) 1980
BmsI GCATC 9 cut(s) 485, 892, 1522, 1843, 1987, 2247, 2384, 2519, 2607
BmuI ACTGGG 1 cut(s) 1980
BoxI GACNNNNGTC 1 cut(s) 504
BpiI GAAGAC 2 cut(s) 172, 1055
BpmI CTGGAG 3 cut(s) 825, 1190, 2563
Bpu10I CCTNAGC 1 cut(s) 2259
BpuEI CTTGAG 2 cut(s) 386, 1497
BpuMI CCSGG 3 cut(s) 848, 1669, 1729
Bsa29I ATCGAT 1 cut(s) 898
BsaHI GRCGYC 2 cut(s) 97, 177
BsaI GGTCTC 2 cut(s) 720, 1769
BsaJI CCNNGG 7 cut(s) 280, 422, 1018, 1803, 2381, 2402, 2621
BsaWI WCCGGW 2 cut(s) 190, 770
BsaXI ACNNNNNCTCC 2 cut(s) 2599, 2629
Bsc4I CCNNNNNNNGG 2 cut(s) 1914, 2403
Bse118I RCCGGY 1 cut(s) 2491
Bse1I ACTGG 7 cut(s) 331, 716, 1003, 1376, 1433, 1875, 1986
Bse21I CCTNAGG 3 cut(s) 65, 888, 2808
Bse3DI GCAATG 2 cut(s) 2063, 2885
BseAI TCCGGA 1 cut(s) 190
BseBI CCWGG 5 cut(s) 241, 423, 1020, 2581, 2622
BseCI ATCGAT 1 cut(s) 898
BseDI CCNNGG 7 cut(s) 280, 422, 1018, 1803, 2381, 2402, 2621
BseGI GGATG 7 cut(s) 73, 883, 898, 1096, 1707, 1922, 2598
BseLI CCNNNNNNNGG 2 cut(s) 1914, 2403
BseMI GCAATG 2 cut(s) 2063, 2885
BseMII CTCAG 9 cut(s) 79, 879, 1241, 1287, 2235, 2273, 2319, 2791, 2799
BseNI ACTGG 7 cut(s) 331, 716, 1003, 1376, 1433, 1875, 1986
BseRI GAGGAG 6 cut(s) 34, 45, 123, 1187, 1498, 2564
BseSI GKGCMC 2 cut(s) 348, 1585
BseXI GCAGC 3 cut(s) 142, 145, 321
BsgI GTGCAG 1 cut(s) 320
Bsh1236I CGCG 1 cut(s) 105
BshFI GGCC 9 cut(s) 406, 656, 1128, 1591, 1612, 1934, 2207, 2386, 2672
BshVI ATCGAT 1 cut(s) 898
BsiHKAI GWGCWC 1 cut(s) 1585
BsiHKCI CYCGRG 1 cut(s) 252
BsiSI CCGG 6 cut(s) 191, 771, 848, 1668, 1728, 2492
BslFI GGGAC 7 cut(s) 439, 941, 1108, 1128, 1434, 1744, 1894
BslI CCNNNNNNNGG 2 cut(s) 1914, 2403
BsmAI GTCTC 7 cut(s) 36, 188, 255, 720, 845, 1169, 1769
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 7 cut(s) 439, 941, 1108, 1128, 1434, 1744, 1894
BsmI GAATGC 4 cut(s) 474, 1960, 2470, 2632
BsnI GGCC 9 cut(s) 406, 656, 1128, 1591, 1612, 1934, 2207, 2386, 2672
Bso31I GGTCTC 2 cut(s) 720, 1769
BsoBI CYCGRG 1 cut(s) 252
Bsp1286I GDGCHC 2 cut(s) 348, 1585
Bsp13I TCCGGA 1 cut(s) 190
Bsp19I CCATGG 1 cut(s) 2381
BspACI CCGC 7 cut(s) 24, 100, 103, 153, 162, 1112, 1426
BspANI GGCC 9 cut(s) 406, 656, 1128, 1591, 1612, 1934, 2207, 2386, 2672
BspCNI CTCAG 9 cut(s) 78, 880, 1240, 1288, 2236, 2272, 2320, 2790, 2800
BspDI ATCGAT 1 cut(s) 898
BspEI TCCGGA 1 cut(s) 190
BspFNI CGCG 1 cut(s) 105
BspHI TCATGA 3 cut(s) 670, 760, 1707
BspLI GGNNCC 4 cut(s) 71, 1287, 1551, 1776
BspPI GGATC 6 cut(s) 284, 1544, 1557, 1769, 1782, 1814
BspTNI GGTCTC 2 cut(s) 720, 1769
BsrDI GCAATG 2 cut(s) 2063, 2885
BsrFI RCCGGY 1 cut(s) 2491
BsrI ACTGG 7 cut(s) 331, 716, 1003, 1376, 1433, 1875, 1986
BssAI RCCGGY 1 cut(s) 2491
BssECI CCNNGG 7 cut(s) 280, 422, 1018, 1803, 2381, 2402, 2621
BssNI GRCGYC 2 cut(s) 97, 177
BssSI CACGAG 1 cut(s) 2469
BssT1I CCWWGG 3 cut(s) 1803, 2381, 2402
Bst2BI CACGAG 1 cut(s) 2469
Bst2UI CCWGG 5 cut(s) 241, 423, 1020, 2581, 2622
Bst4CI ACNGT 7 cut(s) 453, 1124, 1413, 1587, 1641, 1735, 2879
Bst6I CTCTTC 9 cut(s) 39, 84, 89, 128, 219, 1458, 1522, 2149, 2847
BstACI GRCGYC 2 cut(s) 97, 177
BstAPI GCANNNNNTGC 2 cut(s) 1981, 2347
BstC8I GCNNGC 7 cut(s) 332, 348, 474, 1265, 2122, 2209, 2343
BstDSI CCRYGG 1 cut(s) 2381
BstF5I GGATG 7 cut(s) 73, 883, 898, 1096, 1707, 1922, 2598
BstFNI CGCG 1 cut(s) 105
BstHHI GCGC 1 cut(s) 107
BstMAI GTCTC 7 cut(s) 36, 188, 255, 720, 845, 1169, 1769
BstNI CCWGG 5 cut(s) 241, 423, 1020, 2581, 2622
BstPAI GACNNNNGTC 1 cut(s) 504
BstSCI CCNGG 8 cut(s) 239, 421, 846, 1018, 1667, 1727, 2579, 2620
BstSFI CTRYAG 2 cut(s) 725, 927
BstSLI GKGCMC 2 cut(s) 348, 1585
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 3 cut(s) 142, 145, 321
BstV2I GAAGAC 2 cut(s) 172, 1055
BstX2I RGATCY 6 cut(s) 1165, 1223, 1327, 1549, 1774, 1819
BstXI CCANNNNNNTGG 3 cut(s) 414, 454, 2076
BstYI RGATCY 6 cut(s) 1165, 1223, 1327, 1549, 1774, 1819
Bsu15I ATCGAT 1 cut(s) 898
Bsu36I CCTNAGG 3 cut(s) 65, 888, 2808
BsuRI GGCC 9 cut(s) 406, 656, 1128, 1591, 1612, 1934, 2207, 2386, 2672
BsuTUI ATCGAT 1 cut(s) 898
BtgI CCRYGG 1 cut(s) 2381
BtgZI GCGATG 1 cut(s) 44
BtsCI GGATG 7 cut(s) 73, 883, 898, 1096, 1707, 1922, 2598
BtsI GCAGTG 1 cut(s) 1274
BtsIMutI CAGTG 7 cut(s) 324, 996, 1274, 1369, 1583, 1868, 2387
Cac8I GCNNGC 7 cut(s) 332, 348, 474, 1265, 2122, 2209, 2343
CaiI CAGNNNCTG 1 cut(s) 2347
CciI TCATGA 3 cut(s) 670, 760, 1707
CfoI GCGC 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 2491
Cfr13I GGNCC 1 cut(s) 1894
ClaI ATCGAT 1 cut(s) 898
CseI GACGC 2 cut(s) 105, 166
CsiI ACCWGGT 1 cut(s) 239
Csp6I GTAC 3 cut(s) 1441, 1599, 2542
CspCI CAANNNNNGTGG 2 cut(s) 1397, 1432
CviQI GTAC 3 cut(s) 1441, 1599, 2542
DraIII CACNNNGTG 1 cut(s) 243
DrdI GACNNNNNNGTC 2 cut(s) 176, 202
DriI GACNNNNNGTC 2 cut(s) 945, 1887
DseDI GACNNNNNNGTC 2 cut(s) 176, 202
EaeI YGGCCR 2 cut(s) 1610, 2384
Eam1104I CTCTTC 9 cut(s) 39, 84, 89, 128, 219, 1458, 1522, 2149, 2847
Eam1105I GACNNNNNGTC 2 cut(s) 945, 1887
EarI CTCTTC 9 cut(s) 39, 84, 89, 128, 219, 1458, 1522, 2149, 2847
EciI GGCGGA 1 cut(s) 13
Eco130I CCWWGG 3 cut(s) 1803, 2381, 2402
Eco147I AGGCCT 3 cut(s) 656, 1934, 2672
Eco31I GGTCTC 2 cut(s) 720, 1769
Eco32I GATATC 1 cut(s) 2701
Eco47I GGWCC 1 cut(s) 1894
Eco81I CCTNAGG 3 cut(s) 65, 888, 2808
Eco88I CYCGRG 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 1894
EcoRII CCWGG 5 cut(s) 239, 421, 1018, 2579, 2620
EcoRV GATATC 1 cut(s) 2701
EcoT14I CCWWGG 3 cut(s) 1803, 2381, 2402
ErhI CCWWGG 3 cut(s) 1803, 2381, 2402
Esp3I CGTCTC 1 cut(s) 36
FaqI GGGAC 7 cut(s) 439, 941, 1108, 1128, 1434, 1744, 1894
FauI CCCGC 1 cut(s) 1105
FauNDI CATATG 1 cut(s) 943
FbaI TGATCA 3 cut(s) 430, 1027, 2632
FblI GTMKAC 1 cut(s) 724
Fnu4HI GCNGC 7 cut(s) 100, 103, 153, 156, 159, 162, 335
FokI GGATG 7 cut(s) 60, 870, 905, 1103, 1714, 1909, 2585
Fsp4HI GCNGC 7 cut(s) 100, 103, 153, 156, 159, 162, 335
FspBI CTAG 6 cut(s) 720, 1877, 2051, 2451, 2585, 2666
GlaI GCGC 1 cut(s) 106
GluI GCNGC 7 cut(s) 100, 103, 153, 156, 159, 162, 335
GsuI CTGGAG 3 cut(s) 825, 1190, 2563
HaeIII GGCC 9 cut(s) 406, 656, 1128, 1591, 1612, 1934, 2207, 2386, 2672
HapII CCGG 6 cut(s) 191, 771, 848, 1668, 1728, 2492
HgaI GACGC 2 cut(s) 105, 166
HhaI GCGC 1 cut(s) 107
Hin1I GRCGYC 2 cut(s) 97, 177
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HincII GTYRAC 2 cut(s) 499, 1623
HindII GTYRAC 2 cut(s) 499, 1623
HindIII AAGCTT 3 cut(s) 271, 974, 1055
HinfI GANTC 6 cut(s) 4, 248, 767, 2108, 2612, 2894
HpaII CCGG 6 cut(s) 191, 771, 848, 1668, 1728, 2492
HphI GGTGA 9 cut(s) 118, 610, 718, 875, 1717, 2092, 2192, 2669, 2860
Hpy166II GTNNAC 7 cut(s) 499, 709, 725, 1583, 1623, 1725, 2644
Hpy8I GTNNAC 7 cut(s) 499, 709, 725, 1583, 1623, 1725, 2644
Hpy99I CGWCG 1 cut(s) 99
HpyAV CCTTC 7 cut(s) 28, 391, 396, 476, 1945, 2320, 2408
HpyCH4III ACNGT 7 cut(s) 453, 1124, 1413, 1587, 1641, 1735, 2879
HpyCH4IV ACGT 2 cut(s) 1557, 2074
HpySE526I ACGT 2 cut(s) 1557, 2074
Hsp92I GRCGYC 2 cut(s) 97, 177
HspAI GCGC 1 cut(s) 105
Kpn2I TCCGGA 1 cut(s) 190
Ksp22I TGATCA 3 cut(s) 430, 1027, 2632
LmnI GCTCC 5 cut(s) 75, 610, 1285, 2044, 2809
Lsp1109I GCAGC 3 cut(s) 142, 145, 321
LweI GCATC 9 cut(s) 485, 892, 1522, 1843, 1987, 2247, 2384, 2519, 2607
MabI ACCWGGT 1 cut(s) 239
MaeI CTAG 6 cut(s) 720, 1877, 2051, 2451, 2585, 2666
MaeII ACGT 2 cut(s) 1557, 2074
MaeIII GTNAC 7 cut(s) 322, 358, 1276, 2113, 2323, 2533, 2908
MflI RGATCY 6 cut(s) 1165, 1223, 1327, 1549, 1774, 1819
MhlI GDGCHC 2 cut(s) 348, 1585
MlsI TGGCCA 2 cut(s) 1612, 2386
MluNI TGGCCA 2 cut(s) 1612, 2386
MlyI GAGTC 2 cut(s) 257, 2102
MmeI TCCRAC 2 cut(s) 192, 2740
Mox20I TGGCCA 2 cut(s) 1612, 2386
MroI TCCGGA 1 cut(s) 190
MscI TGGCCA 2 cut(s) 1612, 2386
MseI TTAA 9 cut(s) 489, 683, 789, 1007, 1101, 1211, 1899, 2295, 2421
MslI CAYNNNNRTG 2 cut(s) 1001, 2380
Msp20I TGGCCA 2 cut(s) 1612, 2386
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 6 cut(s) 191, 771, 848, 1668, 1728, 2492
MspR9I CCNGG 8 cut(s) 241, 423, 848, 1020, 1669, 1729, 2581, 2622
Mva1269I GAATGC 4 cut(s) 474, 1960, 2470, 2632
MvaI CCWGG 5 cut(s) 241, 423, 1020, 2581, 2622
MvnI CGCG 1 cut(s) 105
NciI CCSGG 3 cut(s) 848, 1669, 1729
NcoI CCATGG 1 cut(s) 2381
NdeI CATATG 1 cut(s) 943
NlaIV GGNNCC 4 cut(s) 71, 1287, 1551, 1776
NmeAIII GCCGAG 1 cut(s) 2752
NmuCI GTSAC 3 cut(s) 322, 358, 2113
OliI CACNNNNGTG 1 cut(s) 1001
PagI TCATGA 3 cut(s) 670, 760, 1707
PceI AGGCCT 3 cut(s) 656, 1934, 2672
PcsI WCGNNNNNNNCGW 3 cut(s) 354, 2467, 2827
PctI GAATGC 4 cut(s) 474, 1960, 2470, 2632
PfeI GAWTC 4 cut(s) 4, 767, 2612, 2894
PkrI GCNGC 7 cut(s) 101, 104, 154, 157, 160, 163, 336
PleI GAGTC 2 cut(s) 256, 2102
PpsI GAGTC 2 cut(s) 256, 2102
PpuMI RGGWCCY 1 cut(s) 1894
PshAI GACNNNNGTC 1 cut(s) 504
PsiI TTATAA 2 cut(s) 1365, 2727
Psp1406I AACGTT 1 cut(s) 2074
Psp5II RGGWCCY 1 cut(s) 1894
Psp6I CCWGG 5 cut(s) 239, 421, 1018, 2579, 2620
PspGI CCWGG 5 cut(s) 239, 421, 1018, 2579, 2620
PspN4I GGNNCC 4 cut(s) 71, 1287, 1551, 1776
PspPI GGNCC 1 cut(s) 1894
PspPPI RGGWCCY 1 cut(s) 1894
PstNI CAGNNNCTG 1 cut(s) 2347
PsuI RGATCY 6 cut(s) 1165, 1223, 1327, 1549, 1774, 1819
RsaI GTAC 3 cut(s) 1442, 1600, 2543
RsaNI GTAC 3 cut(s) 1441, 1599, 2542
RseI CAYNNNNRTG 2 cut(s) 1001, 2380
SaqAI TTAA 9 cut(s) 489, 683, 789, 1007, 1101, 1211, 1899, 2295, 2421
SatI GCNGC 7 cut(s) 100, 103, 153, 156, 159, 162, 335
Sau96I GGNCC 1 cut(s) 1894
ScaI AGTACT 1 cut(s) 1600
SchI GAGTC 2 cut(s) 257, 2102
ScrFI CCNGG 8 cut(s) 241, 423, 848, 1020, 1669, 1729, 2581, 2622
SduI GDGCHC 2 cut(s) 348, 1585
SexAI ACCWGGT 1 cut(s) 239
SfaNI GCATC 9 cut(s) 485, 892, 1522, 1843, 1987, 2247, 2384, 2519, 2607
SfcI CTRYAG 2 cut(s) 725, 927
SinI GGWCC 1 cut(s) 1894
SmiMI CAYNNNNRTG 2 cut(s) 1001, 2380
SmlI CTYRAG 2 cut(s) 365, 1512
SmoI CTYRAG 2 cut(s) 365, 1512
SseBI AGGCCT 3 cut(s) 656, 1934, 2672
SsiI CCGC 7 cut(s) 24, 100, 103, 153, 162, 1112, 1426
SspI AATATT 1 cut(s) 2480
SspMI CTAG 6 cut(s) 720, 1877, 2051, 2451, 2585, 2666
StuI AGGCCT 3 cut(s) 656, 1934, 2672
StyD4I CCNGG 8 cut(s) 239, 421, 846, 1018, 1667, 1727, 2579, 2620
StyI CCWWGG 3 cut(s) 1803, 2381, 2402
TaaI ACNGT 7 cut(s) 453, 1124, 1413, 1587, 1641, 1735, 2879
TaiI ACGT 2 cut(s) 1560, 2077
TaqI TCGA 6 cut(s) 508, 640, 898, 2417, 2830, 2897
TaqII GACCGA 1 cut(s) 294
TatI WGTACW 2 cut(s) 1598, 2541
TauI GCSGC 4 cut(s) 102, 105, 155, 164
TfiI GAWTC 4 cut(s) 4, 767, 2612, 2894
Tru1I TTAA 9 cut(s) 489, 683, 789, 1007, 1101, 1211, 1899, 2295, 2421
Tru9I TTAA 9 cut(s) 489, 683, 789, 1007, 1101, 1211, 1899, 2295, 2421
TscAI CASTG 7 cut(s) 331, 1003, 1274, 1376, 1590, 1875, 2394
TseFI GTSAC 3 cut(s) 322, 358, 2113
TseI GCWGC 3 cut(s) 155, 158, 334
Tsp45I GTSAC 3 cut(s) 322, 358, 2113
TspGWI ACGGA 1 cut(s) 1860
TspRI CASTG 7 cut(s) 331, 1003, 1274, 1376, 1590, 1875, 2394
VneI GTGCAC 1 cut(s) 1581
VpaK11BI GGWCC 1 cut(s) 1894
XapI RAATTY 4 cut(s) 1978, 2132, 2434, 2789
XbaI TCTAGA 1 cut(s) 2050
XcmI CCANNNNNNNNNTGG 1 cut(s) 2651
XmiI GTMKAC 1 cut(s) 724
XspI CTAG 6 cut(s) 720, 1877, 2051, 2451, 2585, 2666
ZrmI AGTACT 1 cut(s) 1600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.