MD09G1264400.v1.1

valine--tRNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004429
Physical Location & Seq
Reverse (-)
33726783 .. 33745073
18291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1264400.v1.1.491

Sequence Viewer

Length: 2652 bp
ATGATTCTCCAAACGACATTCCCCGGCCCCTCTCTCTGCTCGTCGTTCTCTGCTCACCGGCTCCGTCCTCTTCTCTTCTCCAGACGACGTCGTGGCCGCACAAGCCTCTCCTACTGGCATTTCAATCCTCTTAAGCCTAGGCACTTCTCAGTTGCTGCTTCCGAGAATCGTGGCTTTACATCTCCAGAGATTGCGAAGACCTTTGATTTTTCTTCAGAAGAACGGATATACAATTGGTGGGAGTCTCAAGGATATTTTAGGCCAAACCATGACCAAGGAAGTGATCCTTTTGTGATCTCAATGCCGCCTCCAAATGTTACTGGCTCCCTACACATGGGGCATGCAATGTTTGTGACTCTTGAGGACATTATGGTTAGATACCATAGGATGAAGGGAAAACCAACACTTTGGCTTCCTGGGACTGACCATGCTGGTATCGCAACTCAGCTGGTTGTGGAAAAAATGCTCGCATCTGAAGGAATTAAAAGGGTTGACTTGGGTCGAGATGAGTTTGTGAGTAAAGTTTGGGAGTGGAAAGAGAAATATGGTGGAACCATCATCAATCAGATCAAAAGACTTGGTGCTTCTTGTGATTGGAATAGAGAACATTTCACCCTTGATGAACAGCTAAGTCGTGCTGTTATTGAGGCCTTTGTCAGACTACATGAGAAAGGTTTAATCTATCAAGGGTCCTACATGGTGAACTGGTCTCCTAGTCTACAGACTGCTGTTTCTGACTTGGAAGTAGAATATCATGAAGAATCCGGAACTCTTTATCATCTTAAGTATCGTGTTGCTGGAGGCCTAAAGTCTGACTATTTGACAATAGCAACAACACGTCCGGAGACATTATTTGGTGATGTAGCTATTGCTGTGCATCCTGAGGATGATCGATATTCTAAATATATTAACAGAATGGCTATAGTGCCGTTGACATATGGCCGTCACGTCCCTATCATCGCTGACAAGCATGTTGATAAAGACTTTGGCACTGGTGTGTTAAAGATAAGCCCTGGACATGATCATAATGATTACAATCTTGCAAGAAAGCTTGGTCTTCCCATACTTAATGTCATGAACAAGGATGGAACACTAAACAAGGTTGCCGGACTGTACTGTGGCCTTGATCGGTTTGAGGCCCGGAAGAAACTTTGGGCAGATTTGGAGGAGACTGGTTTAGCTGTAAAGAAAGAACCACACACCCTACGAGTTCCCAGATCTCAGCGTGGTGGAGAAGTTATAGAGCCACTAGTAAGCAAGCAGTGGTTTGTAACGATGGAGCCCTTAGCCGACAAAGCCCTTAGTGCTGTTGAAAAAGGAGACTTGAAAATTATACCTGAAAGATTTGAAAAGATTTACAATCATTGGCTATCGAATATTAAGGATTGGTGCATAAGCAGACAACTGTGGTGGGGACATCGAATACCAGTTTGGTACATTGTGGGTAAAGACTGTGAAGAAGAATATATAGTTGCAAGGAGTGAGGATGAAGCTCTTGGGAAAGCTCAAGAAAAATACGGAAAAGATGTGAAAATATATCAGGATCCGGATGTTCTTGACACATGGTTTTCGAGTGCACTGTGGCCTTTCAGTACTCTTGGATGGCCAGATGAGTCAGTTGAGGATTTTAAGCGGTTTTATCCTACAACAATGCTTGAAACTGGGCATGACATATTGTTCTTCTGGGTTGCGAGGATGGTCATGATGGGAATTGAGTTCACAGGGACAGTGCCATTTAAATATATTTATTTACACGGTCTCATCAGGGATTCACAAGGCCGAAAGATGTCGAAAACATTGGGGAATGTAATAGATCCATTAGACACAATCAAGGAATATGGCACAGATGCTTTAAGATTTACAATTGCTCTAGGACTGCTGGCCAGGTTTGACCTTAATTTATCCACTGAGAGGTTGACATCCAACAAGGCCTTCACTAATAAACTGTGGAATGCTGGAAAGTTTGTATTGCAGAATTTACCCAGTCAAAGCGAAGCATCAACTTGGGAAAATATACTTTCTTATAAGTTTGATAAAGTGGATCTCCTAGACAAGCTACCCTTACCAGAATCTTGGGTGATTTCAAAACTTCATTTGCTTATTGACACAGTCACCGCAAGCTATGACAAATTTTTCTTTGGAGATGTTGGAAGAGAGACGTATGAATTCTTTTGGGGTGATTTTGCTGATTGGTATATAGAGGCCAGCAAAGCTCGTCTTTACCACTCTGGTGGTGATTCAGTTGCTTCTGTCACACAGGCAGTTCTATTGTATGTTTTTGAAAACATACTTAAATTGCTACACCCCTTTATGCCATTTGTGACCGAGGAACTCTGGCAGGCACTCCCCTTTCGGAAAGAAGCTCTTATTGTATCTCCATGGCCACTGACTTCACTTCCAAGGCAGAGCAACTCGATTAAAAAGTTTGAGAATTTACAGGCATTGACTAGAGCTATCAGAAATGCTCGAGCAGAATATTCTGTTGAGCCTGTAAAGCGTATATCAGCATCAATAGTTGCAAATGAAGAAGTCACTGAATACATAATGAAAGAAAAGGAGGTTTTAACTCTTCTGTCCAGGCTGGATTTGCAAAACGTCCATTTTACAGATTCTCCTCCAGGCACATTTCTTCATGGATTATTTTACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 Val_tRNA-synt_C tRNA-synt_1

Protein Analysis

884

Amino Acids

101.53

Weight (kDa)

6.64

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 76 - 624 5.9e-151 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 98 - 250 1.3e-08 tRNA synthetases class I (M)
tRNA-synt_1_2 PF13603 304 - 363 3.9e-07 Leucyl-tRNA synthetase, editing domain
tRNA-synt_1g PF09334 396 - 651 2.4e-11 tRNA synthetases class I (M)
Anticodon_1 PF08264 691 - 834 3.4e-39 Anticodon-binding domain of tRNA ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2025
AatII GACGTC 1 cut(s) 91
AccI GTMKAC 1 cut(s) 718
AccIII TCCGGA 3 cut(s) 764, 841, 1546
AciI CCGC 4 cut(s) 97, 305, 1633, 2115
AclWI GGATC 5 cut(s) 278, 1538, 1551, 1808, 2049
AcoI YGGCCR 5 cut(s) 94, 940, 1604, 1881, 2381
AcsI RAATTY 4 cut(s) 1975, 2129, 2165, 2431
AcuI CTGAAG 2 cut(s) 198, 495
AcyI GRCGYC 1 cut(s) 88
AfaI GTAC 3 cut(s) 1115, 1436, 1594
AfiI CCNNNNNNNGG 2 cut(s) 334, 1911
AflII CTTAAG 2 cut(s) 131, 782
AflIII ACRYGT 1 cut(s) 836
AgsI TTSAA 7 cut(s) 124, 1313, 1327, 1349, 1658, 2085, 2282
AhlI ACTAGT 1 cut(s) 1249
AjiI CACGTC 2 cut(s) 839, 949
AjnI CCWGG 5 cut(s) 415, 1012, 1883, 2576, 2617
AjuI GAANNNNNNNTTGG 4 cut(s) 1414, 1446, 1916, 1948
AleI CACNNNNGTG 2 cut(s) 995, 2229
Alw21I GWGCWC 1 cut(s) 1579
Alw26I GTCTC 7 cut(s) 249, 714, 839, 1163, 1314, 1763, 2150
Alw44I GTGCAC 1 cut(s) 1575
AlwI GGATC 5 cut(s) 278, 1538, 1551, 1808, 2049
AlwNI CAGNNNCTG 1 cut(s) 155
Ama87I CYCGRG 1 cut(s) 2466
Aor13HI TCCGGA 3 cut(s) 764, 841, 1546
ApaLI GTGCAC 1 cut(s) 1575
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 4 cut(s) 1975, 2129, 2165, 2431
AspA2I CCTAGG 1 cut(s) 137
AspS9I GGNCC 3 cut(s) 26, 690, 1138
AsuC2I CCSGG 2 cut(s) 24, 1141
AsuHPI GGTGA 8 cut(s) 47, 604, 712, 869, 2089, 2104, 2189, 2246
AvaI CYCGRG 1 cut(s) 2466
AvaII GGWCC 1 cut(s) 690
AvrII CCTAGG 1 cut(s) 137
AxyI CCTNAGG 1 cut(s) 882
BaeGI GKGCMC 1 cut(s) 1579
BalI TGGCCA 3 cut(s) 1606, 1883, 2383
BamHI GGATCC 1 cut(s) 1543
BanII GRGCYC 1 cut(s) 1284
BbsI GAAGAC 2 cut(s) 203, 1049
Bbv12I GWGCWC 1 cut(s) 1579
BbvI GCAGC 1 cut(s) 142
BccI CCATC 6 cut(s) 563, 1079, 1270, 1596, 1690, 1699
BceAI ACGGC 2 cut(s) 913, 927
BciT130I CCWGG 5 cut(s) 417, 1014, 1885, 2578, 2619
BclI TGATCA 1 cut(s) 1021
BcnI CCSGG 2 cut(s) 24, 1141
BcoDI GTCTC 7 cut(s) 249, 714, 839, 1163, 1314, 1763, 2150
BcuI ACTAGT 1 cut(s) 1249
BfaI CTAG 6 cut(s) 138, 714, 1250, 1871, 2048, 2448
BfmI CTRYAG 2 cut(s) 719, 921
BfrI CTTAAG 2 cut(s) 131, 782
BglII AGATCT 1 cut(s) 1217
BisI GCNGC 3 cut(s) 97, 156, 305
BlnI CCTAGG 1 cut(s) 137
BlsI GCNGC 3 cut(s) 98, 157, 306
BmcAI AGTACT 1 cut(s) 1594
Bme1390I CCNGG 7 cut(s) 24, 417, 1014, 1141, 1885, 2578, 2619
Bme18I GGWCC 1 cut(s) 690
BmeT110I CYCGRG 1 cut(s) 2466
BmgBI CACGTC 2 cut(s) 839, 949
BmgT120I GGNCC 3 cut(s) 26, 690, 1138
BmiI GGNNCC 7 cut(s) 28, 62, 325, 553, 691, 1281, 1545
BmrFI CCNGG 7 cut(s) 24, 417, 1014, 1141, 1885, 2578, 2619
BmrI ACTGGG 2 cut(s) 1671, 1977
BmsI GCATC 5 cut(s) 479, 886, 1837, 2006, 2516
BmuI ACTGGG 2 cut(s) 1671, 1977
BoxI GACNNNNGTC 1 cut(s) 498
BpiI GAAGAC 2 cut(s) 203, 1049
BpmI CTGGAG 4 cut(s) 64, 168, 819, 2601
Bpu10I CCTNAGC 1 cut(s) 1285
BpuEI CTTGAG 3 cut(s) 231, 380, 1491
BpuMI CCSGG 2 cut(s) 24, 1141
Bsa29I ATCGAT 1 cut(s) 892
BsaBI GATNNNNATC 1 cut(s) 1035
BsaHI GRCGYC 1 cut(s) 88
BsaI GGTCTC 2 cut(s) 714, 1763
BsaJI CCNNGG 8 cut(s) 22, 137, 274, 416, 1012, 2325, 2378, 2399
BsaWI WCCGGW 3 cut(s) 764, 841, 1546
BsaXI ACNNNNNCTCC 2 cut(s) 2596, 2626
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 1911
Bse118I RCCGGY 1 cut(s) 57
Bse1I ACTGG 8 cut(s) 119, 325, 710, 997, 1177, 1427, 1666, 1983
Bse21I CCTNAGG 1 cut(s) 882
Bse3DI GCAATG 1 cut(s) 351
Bse8I GATNNNNATC 1 cut(s) 1035
BseAI TCCGGA 3 cut(s) 764, 841, 1546
BseBI CCWGG 5 cut(s) 417, 1014, 1885, 2578, 2619
BseCI ATCGAT 1 cut(s) 892
BseDI CCNNGG 8 cut(s) 22, 137, 274, 416, 1012, 2325, 2378, 2399
BseGI GGATG 9 cut(s) 393, 877, 892, 1090, 1492, 1555, 1607, 1701, 1919
BseJI GATNNNNATC 1 cut(s) 1035
BseLI CCNNNNNNNGG 2 cut(s) 334, 1911
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 5 cut(s) 162, 458, 873, 1235, 1899
BseNI ACTGG 8 cut(s) 119, 325, 710, 997, 1177, 1427, 1666, 1983
BseRI GAGGAG 2 cut(s) 1181, 2604
BseSI GKGCMC 1 cut(s) 1579
BseXI GCAGC 1 cut(s) 142
BshVI ATCGAT 1 cut(s) 892
BsiHKAI GWGCWC 1 cut(s) 1579
BsiHKCI CYCGRG 1 cut(s) 2466
BsiSI CCGG 7 cut(s) 24, 58, 765, 842, 1107, 1141, 1547
BslFI GGGAC 4 cut(s) 433, 935, 1428, 1738
BslI CCNNNNNNNGG 2 cut(s) 334, 1911
BsmAI GTCTC 7 cut(s) 249, 714, 839, 1163, 1314, 1763, 2150
BsmBI CGTCTC 1 cut(s) 2150
BsmFI GGGAC 4 cut(s) 433, 935, 1428, 1738
BsmI GAATGC 1 cut(s) 1957
Bso31I GGTCTC 2 cut(s) 714, 1763
BsoBI CYCGRG 1 cut(s) 2466
Bsp1286I GDGCHC 2 cut(s) 1284, 1579
Bsp13I TCCGGA 3 cut(s) 764, 841, 1546
Bsp19I CCATGG 1 cut(s) 2378
BspACI CCGC 4 cut(s) 97, 305, 1633, 2115
BspCNI CTCAG 5 cut(s) 161, 457, 874, 1234, 1900
BspDI ATCGAT 1 cut(s) 892
BspEI TCCGGA 3 cut(s) 764, 841, 1546
BspHI TCATGA 3 cut(s) 754, 1074, 1701
BspLI GGNNCC 7 cut(s) 28, 62, 325, 553, 691, 1281, 1545
BspPI GGATC 5 cut(s) 278, 1538, 1551, 1808, 2049
BspTI CTTAAG 2 cut(s) 131, 782
BspTNI GGTCTC 2 cut(s) 714, 1763
BsrDI GCAATG 1 cut(s) 351
BsrFI RCCGGY 1 cut(s) 57
BsrI ACTGG 8 cut(s) 119, 325, 710, 997, 1177, 1427, 1666, 1983
BssAI RCCGGY 1 cut(s) 57
BssECI CCNNGG 8 cut(s) 22, 137, 274, 416, 1012, 2325, 2378, 2399
BssNI GRCGYC 1 cut(s) 88
BssT1I CCWWGG 4 cut(s) 137, 274, 2378, 2399
Bst2UI CCWGG 5 cut(s) 417, 1014, 1885, 2578, 2619
Bst4CI ACNGT 9 cut(s) 1113, 1118, 1407, 1454, 1581, 1729, 1757, 1947, 2110
Bst6I CTCTTC 4 cut(s) 75, 80, 2146, 2574
BstACI GRCGYC 1 cut(s) 88
BstAFI CTTAAG 2 cut(s) 131, 782
BstC8I GCNNGC 7 cut(s) 342, 468, 1259, 1881, 2119, 2206, 2340
BstDEI CTNAG 8 cut(s) 148, 444, 629, 882, 1221, 1285, 1301, 1908
BstDSI CCRYGG 1 cut(s) 2378
BstF5I GGATG 9 cut(s) 393, 877, 892, 1090, 1492, 1555, 1607, 1701, 1919
BstMAI GTCTC 7 cut(s) 249, 714, 839, 1163, 1314, 1763, 2150
BstMWI GCNNNNNNNGC 7 cut(s) 102, 437, 1295, 1304, 2210, 2494, 2587
BstNI CCWGG 5 cut(s) 417, 1014, 1885, 2578, 2619
BstNSI RCATGY 2 cut(s) 344, 974
BstPAI GACNNNNGTC 1 cut(s) 498
BstSCI CCNGG 7 cut(s) 22, 415, 1012, 1139, 1883, 2576, 2617
BstSFI CTRYAG 2 cut(s) 719, 921
BstSLI GKGCMC 1 cut(s) 1579
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 2 cut(s) 203, 1049
BstX2I RGATCY 4 cut(s) 1217, 1543, 1813, 2041
BstXI CCANNNNNNTGG 3 cut(s) 408, 2073, 2231
BstYI RGATCY 4 cut(s) 1217, 1543, 1813, 2041
Bsu15I ATCGAT 1 cut(s) 892
Bsu36I CCTNAGG 1 cut(s) 882
BsuTUI ATCGAT 1 cut(s) 892
BtgI CCRYGG 1 cut(s) 2378
BtgZI GCGATG 1 cut(s) 943
BtrI CACGTC 2 cut(s) 839, 949
BtsCI GGATG 9 cut(s) 393, 877, 892, 1090, 1492, 1555, 1607, 1701, 1919
BtsI GCAGTG 1 cut(s) 1268
BtsIMutI CAGTG 7 cut(s) 990, 1268, 1577, 1734, 1905, 2384, 2532
Cac8I GCNNGC 7 cut(s) 342, 468, 1259, 1881, 2119, 2206, 2340
CaiI CAGNNNCTG 1 cut(s) 155
CciI TCATGA 3 cut(s) 754, 1074, 1701
Cfr10I RCCGGY 1 cut(s) 57
Cfr13I GGNCC 3 cut(s) 26, 690, 1138
ClaI ATCGAT 1 cut(s) 892
Csp6I GTAC 3 cut(s) 1114, 1435, 1593
CspCI CAANNNNNGTGG 2 cut(s) 1391, 1426
CviQI GTAC 3 cut(s) 1114, 1435, 1593
DdeI CTNAG 8 cut(s) 148, 444, 629, 882, 1221, 1285, 1301, 1908
DraI TTTAAA 1 cut(s) 1738
EaeI YGGCCR 5 cut(s) 94, 940, 1604, 1881, 2381
Eam1104I CTCTTC 4 cut(s) 75, 80, 2146, 2574
EarI CTCTTC 4 cut(s) 75, 80, 2146, 2574
Eco130I CCWWGG 4 cut(s) 137, 274, 2378, 2399
Eco147I AGGCCT 3 cut(s) 650, 804, 1931
Eco24I GRGCYC 1 cut(s) 1284
Eco31I GGTCTC 2 cut(s) 714, 1763
Eco47I GGWCC 1 cut(s) 690
Eco57I CTGAAG 2 cut(s) 198, 495
Eco81I CCTNAGG 1 cut(s) 882
Eco88I CYCGRG 1 cut(s) 2466
EcoO109I RGGNCCY 1 cut(s) 690
EcoRI GAATTC 1 cut(s) 2165
EcoRII CCWGG 5 cut(s) 415, 1012, 1883, 2576, 2617
EcoT14I CCWWGG 4 cut(s) 137, 274, 2378, 2399
EcoT38I GRGCYC 1 cut(s) 1284
ErhI CCWWGG 4 cut(s) 137, 274, 2378, 2399
Esp3I CGTCTC 1 cut(s) 2150
FalI AAGNNNNNCTT 8 cut(s) 271, 303, 2045, 2077, 2202, 2234, 2349, 2381
FaqI GGGAC 4 cut(s) 433, 935, 1428, 1738
FauNDI CATATG 1 cut(s) 937
FbaI TGATCA 1 cut(s) 1021
FblI GTMKAC 1 cut(s) 718
Fnu4HI GCNGC 3 cut(s) 97, 156, 305
FokI GGATG 9 cut(s) 400, 864, 899, 1097, 1499, 1562, 1614, 1708, 1906
FriOI GRGCYC 1 cut(s) 1284
Fsp4HI GCNGC 3 cut(s) 97, 156, 305
FspBI CTAG 6 cut(s) 138, 714, 1250, 1871, 2048, 2448
GluI GCNGC 3 cut(s) 97, 156, 305
GsuI CTGGAG 4 cut(s) 64, 168, 819, 2601
HapII CCGG 7 cut(s) 24, 58, 765, 842, 1107, 1141, 1547
Hin1I GRCGYC 1 cut(s) 88
HincII GTYRAC 3 cut(s) 493, 933, 1917
HindII GTYRAC 3 cut(s) 493, 933, 1917
HindIII AAGCTT 1 cut(s) 1049
HpaII CCGG 7 cut(s) 24, 58, 765, 842, 1107, 1141, 1547
HphI GGTGA 8 cut(s) 47, 604, 712, 869, 2089, 2104, 2189, 2246
Hpy166II GTNNAC 7 cut(s) 493, 703, 719, 933, 1577, 1719, 1917
Hpy188I TCNGA 9 cut(s) 163, 217, 475, 567, 659, 736, 814, 2355, 2459
Hpy8I GTNNAC 7 cut(s) 493, 703, 719, 933, 1577, 1719, 1917
Hpy99I CGWCG 3 cut(s) 46, 90, 93
HpyAV CCTTC 3 cut(s) 385, 470, 1942
HpyCH4III ACNGT 9 cut(s) 1113, 1118, 1407, 1454, 1581, 1729, 1757, 1947, 2110
HpyCH4IV ACGT 5 cut(s) 88, 838, 948, 2159, 2595
HpyCH4V TGCA 9 cut(s) 344, 877, 1043, 1392, 1475, 1577, 1972, 2519, 2590
HpyF10VI GCNNNNNNNGC 7 cut(s) 102, 437, 1295, 1304, 2210, 2494, 2587
HpyF3I CTNAG 8 cut(s) 148, 444, 629, 882, 1221, 1285, 1301, 1908
HpySE526I ACGT 5 cut(s) 88, 838, 948, 2159, 2595
Hsp92I GRCGYC 1 cut(s) 88
Kpn2I TCCGGA 3 cut(s) 764, 841, 1546
Ksp22I TGATCA 1 cut(s) 1021
LmnI GCTCC 3 cut(s) 66, 329, 1279
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 5 cut(s) 479, 886, 1837, 2006, 2516
MaeI CTAG 6 cut(s) 138, 714, 1250, 1871, 2048, 2448
MaeII ACGT 5 cut(s) 88, 838, 948, 2159, 2595
MaeIII GTNAC 8 cut(s) 316, 352, 944, 1270, 2110, 2251, 2320, 2530
MfeI CAATTG 2 cut(s) 232, 1863
MflI RGATCY 4 cut(s) 1217, 1543, 1813, 2041
MhlI GDGCHC 2 cut(s) 1284, 1579
MlsI TGGCCA 3 cut(s) 1606, 1883, 2383
MluNI TGGCCA 3 cut(s) 1606, 1883, 2383
MlyI GAGTC 3 cut(s) 251, 349, 1622
MmeI TCCRAC 2 cut(s) 1947, 2128
Mox20I TGGCCA 3 cut(s) 1606, 1883, 2383
MroI TCCGGA 3 cut(s) 764, 841, 1546
MscI TGGCCA 3 cut(s) 1606, 1883, 2383
MslI CAYNNNNRTG 2 cut(s) 995, 2229
Msp20I TGGCCA 3 cut(s) 1606, 1883, 2383
MspA1I CMGCKG 1 cut(s) 448
MspCI CTTAAG 2 cut(s) 131, 782
MspI CCGG 7 cut(s) 24, 58, 765, 842, 1107, 1141, 1547
MspR9I CCNGG 7 cut(s) 24, 417, 1014, 1141, 1885, 2578, 2619
MunI CAATTG 2 cut(s) 232, 1863
Mva1269I GAATGC 1 cut(s) 1957
MvaI CCWGG 5 cut(s) 417, 1014, 1885, 2578, 2619
MwoI GCNNNNNNNGC 7 cut(s) 102, 437, 1295, 1304, 2210, 2494, 2587
NciI CCSGG 2 cut(s) 24, 1141
NcoI CCATGG 1 cut(s) 2378
NdeI CATATG 1 cut(s) 937
NlaIV GGNNCC 7 cut(s) 28, 62, 325, 553, 691, 1281, 1545
NmuCI GTSAC 6 cut(s) 352, 944, 2110, 2251, 2320, 2530
NspI RCATGY 2 cut(s) 344, 974
OliI CACNNNNGTG 2 cut(s) 995, 2229
PaeI GCATGC 1 cut(s) 344
PaeR7I CTCGAG 1 cut(s) 2466
PagI TCATGA 3 cut(s) 754, 1074, 1701
PceI AGGCCT 3 cut(s) 650, 804, 1931
PctI GAATGC 1 cut(s) 1957
PfeI GAWTC 7 cut(s) 4, 166, 761, 1769, 2069, 2237, 2609
PflFI GACNNNGTC 2 cut(s) 87, 2108
PkrI GCNGC 3 cut(s) 98, 157, 306
PleI GAGTC 3 cut(s) 250, 349, 1621
PpsI GAGTC 3 cut(s) 250, 349, 1621
PpuMI RGGWCCY 1 cut(s) 690
PshAI GACNNNNGTC 1 cut(s) 498
PsiI TTATAA 1 cut(s) 2025
Psp5II RGGWCCY 1 cut(s) 690
Psp6I CCWGG 5 cut(s) 415, 1012, 1883, 2576, 2617
PspGI CCWGG 5 cut(s) 415, 1012, 1883, 2576, 2617
PspN4I GGNNCC 7 cut(s) 28, 62, 325, 553, 691, 1281, 1545
PspPI GGNCC 3 cut(s) 26, 690, 1138
PspPPI RGGWCCY 1 cut(s) 690
PspXI VCTCGAGB 1 cut(s) 2466
PstNI CAGNNNCTG 1 cut(s) 155
PsuI RGATCY 4 cut(s) 1217, 1543, 1813, 2041
PsyI GACNNNGTC 2 cut(s) 87, 2108
PvuII CAGCTG 1 cut(s) 448
RsaI GTAC 3 cut(s) 1115, 1436, 1594
RsaNI GTAC 3 cut(s) 1114, 1435, 1593
RseI CAYNNNNRTG 2 cut(s) 995, 2229
SatI GCNGC 3 cut(s) 97, 156, 305
Sau96I GGNCC 3 cut(s) 26, 690, 1138
ScaI AGTACT 1 cut(s) 1594
SchI GAGTC 3 cut(s) 251, 349, 1622
ScrFI CCNGG 7 cut(s) 24, 417, 1014, 1141, 1885, 2578, 2619
SduI GDGCHC 2 cut(s) 1284, 1579
SfaNI GCATC 5 cut(s) 479, 886, 1837, 2006, 2516
SfcI CTRYAG 2 cut(s) 719, 921
Sfr274I CTCGAG 1 cut(s) 2466
SinI GGWCC 1 cut(s) 690
SlaI CTCGAG 1 cut(s) 2466
SmiI ATTTAAAT 1 cut(s) 1738
SmiMI CAYNNNNRTG 2 cut(s) 995, 2229
SmlI CTYRAG 6 cut(s) 131, 246, 359, 782, 1506, 2466
SmoI CTYRAG 6 cut(s) 131, 246, 359, 782, 1506, 2466
SpeI ACTAGT 1 cut(s) 1249
SphI GCATGC 1 cut(s) 344
SseBI AGGCCT 3 cut(s) 650, 804, 1931
SsiI CCGC 4 cut(s) 97, 305, 1633, 2115
SspI AATATT 2 cut(s) 1378, 2477
SspMI CTAG 6 cut(s) 138, 714, 1250, 1871, 2048, 2448
StuI AGGCCT 3 cut(s) 650, 804, 1931
StyD4I CCNGG 7 cut(s) 22, 415, 1012, 1139, 1883, 2576, 2617
StyI CCWWGG 4 cut(s) 137, 274, 2378, 2399
SwaI ATTTAAAT 1 cut(s) 1738
TaaI ACNGT 9 cut(s) 1113, 1118, 1407, 1454, 1581, 1729, 1757, 1947, 2110
TaiI ACGT 5 cut(s) 91, 841, 951, 2162, 2598
TaqI TCGA 8 cut(s) 502, 892, 1373, 1420, 1571, 1790, 2414, 2467
TaqII GACCGA 1 cut(s) 2339
TatI WGTACW 2 cut(s) 1113, 1592
TauI GCSGC 2 cut(s) 99, 307
TfiI GAWTC 7 cut(s) 4, 166, 761, 1769, 2069, 2237, 2609
TscAI CASTG 7 cut(s) 997, 1268, 1584, 1734, 1912, 2391, 2539
TseFI GTSAC 6 cut(s) 352, 944, 2110, 2251, 2320, 2530
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 6 cut(s) 352, 944, 2110, 2251, 2320, 2530
TspGWI ACGGA 3 cut(s) 53, 238, 1533
TspRI CASTG 7 cut(s) 997, 1268, 1584, 1734, 1912, 2391, 2539
Tth111I GACNNNGTC 2 cut(s) 87, 2108
Vha464I CTTAAG 2 cut(s) 131, 782
VneI GTGCAC 1 cut(s) 1575
VpaK11BI GGWCC 1 cut(s) 690
XapI RAATTY 4 cut(s) 1975, 2129, 2165, 2431
XceI RCATGY 2 cut(s) 344, 974
XhoI CTCGAG 1 cut(s) 2466
XmaJI CCTAGG 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 718
XspI CTAG 6 cut(s) 138, 714, 1250, 1871, 2048, 2448
ZraI GACGTC 1 cut(s) 89
ZrmI AGTACT 1 cut(s) 1594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.