MD17G1258800.v1.1

valine--tRNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004429
Physical Location & Seq
Reverse (-)
31911162 .. 31912720
1559 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1258800.v1.1.491

Sequence Viewer

Length: 558 bp
ATGCCATTTGTGACCGAGGAACTCTGGCAGACTAGAGCTATCAGAAATGCTCGAGCAGAATATTCTGTTGAGCCTGTAAAGCGTATATCAGCATCTATAGTTGCAACTGAAGAAGTCACTGAATACATATGGAAAGAAAAAGAAGTTTTAGCTCTTCTCTCCAGGCTAGATTTACAAAACATCAATTTCACCGATTCTCCCCCAGGCACGTTTCTTCATCGATTATTTTCCATTTGGCCAAGGCCCGGAAATGTTCATGTTTATTGGAATGCGGATCAATCTGTCCATCTTGTAGCTGGTGAAGGGCTAGAGGCATATCTCCCTCTTGCTGACATGATTGATATTACTGCCGAAATCCAACGCCTATCTAAGAGACTTTCCAAGATGCAGACAGAATATGATGGACTTAAAGCTCGGCTCAGCTCTCCTAAATTTGTGGAGAAAGCTCCTGAAGATATTGTCCGTGGAGTTCAAGAAAAGGCGGCAGAAACAGAAGAGAAGATTACTCTCACCAAAAACCGATTAGCATTGCTCGAATCAAGTGTTCTGGTCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 Val_tRNA-synt_C tRNA-synt_1

Protein Analysis

186

Amino Acids

21.05

Weight (kDa)

5.52

Isoelectric Point (pI)

51.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Val_tRNA-synt_C PF10458 114 - 178 5.3e-15 Valyl tRNA synthetase tRNA binding arm
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 272, 482
AclWI GGATC 1 cut(s) 282
AcoI YGGCCR 1 cut(s) 236
AcsI RAATTY 1 cut(s) 431
AcuI CTGAAG 2 cut(s) 129, 471
AfiI CCNNNNNNNGG 1 cut(s) 245
AgsI TTSAA 1 cut(s) 473
AjnI CCWGG 2 cut(s) 161, 202
AluBI AGCT 6 cut(s) 38, 152, 296, 413, 423, 446
AluI AGCT 6 cut(s) 38, 152, 296, 413, 423, 446
Alw26I GTCTC 1 cut(s) 367
AlwI GGATC 1 cut(s) 282
Ama87I CYCGRG 1 cut(s) 51
AoxI GGCC 2 cut(s) 236, 242
ApoI RAATTY 1 cut(s) 431
AspS9I GGNCC 1 cut(s) 243
AsuC2I CCSGG 1 cut(s) 246
AsuHPI GGTGA 3 cut(s) 181, 311, 502
AvaI CYCGRG 1 cut(s) 51
BalI TGGCCA 1 cut(s) 238
BccI CCATC 2 cut(s) 294, 395
BciT130I CCWGG 2 cut(s) 163, 204
BcnI CCSGG 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 3 cut(s) 33, 167, 308
BfmI CTRYAG 1 cut(s) 96
BisI GCNGC 1 cut(s) 483
BlpI GCTNAGC 1 cut(s) 419
BlsI GCNGC 1 cut(s) 484
Bme1390I CCNGG 3 cut(s) 163, 204, 246
BmeT110I CYCGRG 1 cut(s) 51
BmgT120I GGNCC 1 cut(s) 243
BmrFI CCNGG 3 cut(s) 163, 204, 246
BmsI GCATC 2 cut(s) 101, 375
BpmI CTGGAG 1 cut(s) 145
Bpu1102I GCTNAGC 1 cut(s) 419
BpuMI CCSGG 1 cut(s) 246
Bsa29I ATCGAT 1 cut(s) 220
BsaJI CCNNGG 4 cut(s) 15, 202, 239, 463
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse3DI GCAATG 1 cut(s) 527
BseBI CCWGG 2 cut(s) 163, 204
BseCI ATCGAT 1 cut(s) 220
BseDI CCNNGG 4 cut(s) 15, 202, 239, 463
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMI GCAATG 1 cut(s) 527
BseMII CTCAG 1 cut(s) 433
BshFI GGCC 2 cut(s) 238, 244
BshVI ATCGAT 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 51
BsiSI CCGG 1 cut(s) 246
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 1 cut(s) 367
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 2 cut(s) 238, 244
BsoBI CYCGRG 1 cut(s) 51
Bsp143I GATC 1 cut(s) 274
Bsp1720I GCTNAGC 1 cut(s) 419
BspACI CCGC 2 cut(s) 272, 482
BspANI GGCC 2 cut(s) 238, 244
BspCNI CTCAG 1 cut(s) 432
BspDI ATCGAT 1 cut(s) 220
BspPI GGATC 1 cut(s) 282
BspQI GCTCTTC 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 527
BssECI CCNNGG 4 cut(s) 15, 202, 239, 463
BssMI GATC 1 cut(s) 274
BssT1I CCWWGG 1 cut(s) 239
Bst2UI CCWGG 2 cut(s) 163, 204
Bst6I CTCTTC 2 cut(s) 159, 489
BstDEI CTNAG 2 cut(s) 369, 419
BstDSI CCRYGG 1 cut(s) 463
BstKTI GATC 1 cut(s) 277
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 1 cut(s) 274
BstMWI GCNNNNNNNGC 1 cut(s) 79
BstNI CCWGG 2 cut(s) 163, 204
BstSCI CCNGG 3 cut(s) 161, 202, 244
BstSFI CTRYAG 1 cut(s) 96
Bsu15I ATCGAT 1 cut(s) 220
BsuRI GGCC 2 cut(s) 238, 244
BsuTUI ATCGAT 1 cut(s) 220
BtgI CCRYGG 1 cut(s) 463
BtsIMutI CAGTG 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 243
ClaI ATCGAT 1 cut(s) 220
CviAII CATG 2 cut(s) 257, 334
DdeI CTNAG 2 cut(s) 369, 419
DpnI GATC 1 cut(s) 276
DpnII GATC 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 236
Eam1104I CTCTTC 2 cut(s) 159, 489
EarI CTCTTC 2 cut(s) 159, 489
Eco130I CCWWGG 1 cut(s) 239
Eco57I CTGAAG 2 cut(s) 129, 471
Eco88I CYCGRG 1 cut(s) 51
EcoRII CCWGG 2 cut(s) 161, 202
EcoT14I CCWWGG 1 cut(s) 239
ErhI CCWWGG 1 cut(s) 239
FaeI CATG 2 cut(s) 260, 337
FaiI YATR 9 cut(s) 86, 98, 128, 130, 258, 316, 335, 399, 556
FatI CATG 2 cut(s) 256, 333
FauNDI CATATG 1 cut(s) 128
Fnu4HI GCNGC 1 cut(s) 483
Fsp4HI GCNGC 1 cut(s) 483
FspBI CTAG 3 cut(s) 33, 167, 308
GluI GCNGC 1 cut(s) 483
GsuI CTGGAG 1 cut(s) 145
HaeIII GGCC 2 cut(s) 238, 244
HapII CCGG 1 cut(s) 246
Hin1II CATG 2 cut(s) 260, 337
HinfI GANTC 2 cut(s) 194, 536
HpaII CCGG 1 cut(s) 246
HphI GGTGA 3 cut(s) 181, 311, 502
Hpy188I TCNGA 1 cut(s) 44
Hpy188III TCNNGA 2 cut(s) 449, 473
HpyAV CCTTC 1 cut(s) 296
HpyCH4IV ACGT 1 cut(s) 209
HpyCH4V TGCA 2 cut(s) 104, 388
HpyF10VI GCNNNNNNNGC 1 cut(s) 79
HpyF3I CTNAG 2 cut(s) 369, 419
HpySE526I ACGT 1 cut(s) 209
Hsp92II CATG 2 cut(s) 260, 337
Kzo9I GATC 1 cut(s) 274
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 1 cut(s) 451
LweI GCATC 2 cut(s) 101, 375
MaeI CTAG 3 cut(s) 33, 167, 308
MaeII ACGT 1 cut(s) 209
MaeIII GTNAC 3 cut(s) 10, 115, 550
MalI GATC 1 cut(s) 276
MboI GATC 1 cut(s) 274
MboII GAAGA 6 cut(s) 122, 146, 206, 464, 506, 511
MlsI TGGCCA 1 cut(s) 238
MluCI AATT 2 cut(s) 184, 431
MluNI TGGCCA 1 cut(s) 238
MmeI TCCRAC 1 cut(s) 382
MnlI CCTC 3 cut(s) 10, 304, 333
Mox20I TGGCCA 1 cut(s) 238
MscI TGGCCA 1 cut(s) 238
MseI TTAA 1 cut(s) 408
Msp20I TGGCCA 1 cut(s) 238
MspI CCGG 1 cut(s) 246
MspR9I CCNGG 3 cut(s) 163, 204, 246
Mva1269I GAATGC 1 cut(s) 274
MvaI CCWGG 2 cut(s) 163, 204
MwoI GCNNNNNNNGC 1 cut(s) 79
NciI CCSGG 1 cut(s) 246
NdeI CATATG 1 cut(s) 128
NdeII GATC 1 cut(s) 274
NlaIII CATG 2 cut(s) 260, 337
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 3 cut(s) 10, 115, 550
PaeR7I CTCGAG 1 cut(s) 51
PciSI GCTCTTC 1 cut(s) 159
PctI GAATGC 1 cut(s) 274
PfeI GAWTC 2 cut(s) 194, 536
PkrI GCNGC 1 cut(s) 484
Psp6I CCWGG 2 cut(s) 161, 202
PspGI CCWGG 2 cut(s) 161, 202
PspPI GGNCC 1 cut(s) 243
PspXI VCTCGAGB 1 cut(s) 51
SapI GCTCTTC 1 cut(s) 159
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 1 cut(s) 483
Sau3AI GATC 1 cut(s) 274
Sau96I GGNCC 1 cut(s) 243
ScrFI CCNGG 3 cut(s) 163, 204, 246
SetI ASST 7 cut(s) 40, 154, 212, 298, 415, 425, 448
SfaNI GCATC 2 cut(s) 101, 375
SfcI CTRYAG 1 cut(s) 96
Sfr274I CTCGAG 1 cut(s) 51
SlaI CTCGAG 1 cut(s) 51
SmlI CTYRAG 1 cut(s) 51
SmoI CTYRAG 1 cut(s) 51
Sse9I AATT 2 cut(s) 184, 431
SsiI CCGC 2 cut(s) 272, 482
SspI AATATT 1 cut(s) 62
SspMI CTAG 3 cut(s) 33, 167, 308
StyD4I CCNGG 3 cut(s) 161, 202, 244
StyI CCWWGG 1 cut(s) 239
TaiI ACGT 1 cut(s) 212
TaqI TCGA 3 cut(s) 52, 220, 534
TaqII GACCGA 1 cut(s) 29
TasI AATT 2 cut(s) 184, 431
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 2 cut(s) 194, 536
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 3 cut(s) 10, 115, 550
Tsp45I GTSAC 3 cut(s) 10, 115, 550
TspDTI ATGAA 2 cut(s) 206, 245
TspGWI ACGGA 1 cut(s) 452
TspRI CASTG 1 cut(s) 124
XapI RAATTY 1 cut(s) 431
XcmI CCANNNNNNNNNTGG 1 cut(s) 293
XhoI CTCGAG 1 cut(s) 51
XspI CTAG 3 cut(s) 33, 167, 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.