FvH4_7g04130

valine--tRNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004429
Physical Location & Seq
Forward (+)
4739709 .. 4761870
22162 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g04130.t6

Sequence Viewer

Length: 2931 bp
ATGATTCTCCACACCACCTTCTCCGCTCCTTCTCTTTTCTCCTGCTGCTCTTCTTATCGTGCCCTCAGGCTCCATCCCTTTCTCTCCCCCAAACGACGCCGCTCCGTCACGCCCAGGCTCTTCACAGTTGTCGCCGCCGCCGCCGCCGAGAATGGCGTCTTTACTTCTCCACAGACTTCCAAGTCCTTCGATTTTGCTAACGAAGAGAGGATTTACAGCTGGTGGGAGTCTCAGGGTTATTTCAGGCCCAACTTCGACCGCGGAACTGATCCTTTTGTGATTTCAATGCCGCCTCCAAATGTGACCGGCTCGCTGCACATGGGGCACGCCATGTTCGTCACTCTTGAGGACATTATGGTTAGATATCATCGTATGAAGGGAAGACCAACACTTTGGGTTCCTGGGACTGATCATGCTGGTATAGCCACACAGTTGGTTGTGGAAAGAATGCTTGCATCAGAAGGAATTAAAAGGGTTGACTTGGGTCGAGAAGAGTTTGTGAAAAGAGTTTGGGAGTGGAAAGAGAAGTATGGTGGAACGATCACAAATCAGATCAAAAGGCTTGGTGCTTCTTGTGATTGGAAGAGGGAGCATTTCACCCTTGATGAACAGCTAAGTCGAGCTGTTGTTGAAGCCTTTGTTAGACTTCATGAGAAAGGCTTAATCTATCAAGGATCTTACATGGTGAATTGGTCTCCTAGTCTACAAACTGCTGTTTCTGATTTGGAAGTAGAATATCATGAAGAATCCGGTAGTCTTTATTATATTAAGTATCGTGTTGCTGGAGGCTCAAAGACTGACTTTCTGACAATAGCAACAACTCGCCCGGAGACTTTATTTGGTGATGTAGCTATTGCTGTGCATCCTGAGGATGAACGATATTCTAAATACATCAATAGAATGGCTATAGTGCCTATGACATATGGTCGCCACGTCCCTATCATCGCTGACAAGCTGGTTGAGAAAGAGTTTGGCACCGGCGTGTTAAAGATAAGTCCCGGACATGATCATAATGATTACAACCTTGCAAGAAAGCTTGGTCTTCCAATACTAAATGTTATGAACAAGGATGGCACACTTAACAAAGTTGCAGGACTATACTGTGGCCTTGATCGGTTTGAGGCCCGAAAGAAACTTTGGGCGGATCTGGAGGAGACAGGATTGGCTGTAAAGAAAGAACCACATACCTTACGAGTTCCCAGATCTCAGCGTGGTGGAGAAGTGATAGAGCCACTAGTAAGCAAGCAGTGGTTTGTAACAATGGAGCCCTTAGCTGAGAAAGCCCTACAAGCAGTTGAAAAAGGAGATCTGAAAATTATACCTGAAAGATTTGACAAGATTTATAATCACTGGCTATCAAATATCAAGGATTGGTGCATAAGCAGACAACTATGGTGGGGACACCGCATACCAGTTTGGTACATTGTGGGTAAAGATTGTGAAGAGGAATATATAGTTGCAAGGAGTTTTGAAGAAGCTCTTGGTAAAGCTCAAGAAAAGTATGGAAGAGATGCAAAAATATATCAGGATCCAGACGTTCTTGATACTTGGTTTTCAAGTGCACTTTGGCCTTTCAGTACTCTTGGGTGGCCGGATGAGTCAACAGAGGATTTTAGACGCTTTTATCCTACATCAATGCTGGAAACTGGGCATGACATATTATTCTTTTGGGTTGCGAGGATGGTGATGATGGGAATTGAGTTCACTGGGACGGTTCCTTTTTCATATATTTATCTACATGGTCTCATCAGAGATCCAGAGGGGCGAAAGATGTCAAAAACCTTGGGGAATGTAATAGATCCTTTAGATACAATCAAAGAATATGGAACAGATGCTTTACGATTTACTCTGGCTCTAGGGACTGCTGGTCAGGACCTTAATTTGTCCACTGAGAGGCTGACATCCAATAAGGCCTTCACTAACAAACTATGGAATGCTGGAAAGTTTGTATTGCAGAATTTGCCCAGCCAGAATGATGCTTCTTGGGAAAGTATACTAATGTATAAGTTTGACAAAGTGGAGCTTCTAGACATTCTACCTTTACCAGAACGTTGGGTGGTCTCGAAACTTCATTTGCTTATCGACTCTGTGACAGCAAGCTATGACAAATTTTTCTTTGGAGATGTAGGAAGAGAGACATACAACTTTTTTTGGGGTGATTTTGCCGACTGGTATATTGAGGCCAGCAAAGCTCGTCTTTCCCAATCTGGTAGTGTCTCTGAGGCTTCTGTTGCTCAGGCAGTTCTATTGTATGTTTTTGAAAACATACTTAAATTGCTACACCCTTTCATGCCATTTGTAACTGAGGAACTCTGGCAGGCACTCCCCAATCGAAAAGAAGCCCTTATAGCATCTCCATGGCCACTGACTTCCCTTCCAAGGCAAATCATCTCAATTAAAAAGTTTGAAAATTTACAGGCCTTGACTAAAGCCATTCGGAATGCACGAGCAGAATATTCTGTTGAGCCGGTAAAGCGTATATCAGCATCAATAGTTGCAAATGCAGAAGTTACTGAATACATAATGAAAGAAAAGGTGGTTTTAGCTCTCCTATCCAGGCTAGATTTGCAAAGCATCAATTTTACAGATTCTCCACCAGTGAATGCCGATCAATCAGTCCACCTTGTTGCTGGTGAAGGACTCGAAGCATATCTTCCTCTTGCGGATATGGTTGATATCACTTCTGAAATCCAACGGCTTCATAAGCGACTTTCCAAAATGCAGACTGAATATGATGGACTCATAGCTCGGCTCAATTCTCCTAAATACCATGTACCTGTTACCACATCTACTTTATCCTATCAGTTCAAAGAGAAAGCTCCTGAGGATATTGTTCGTGGAGTTCAAGAAAAAGCAGCAGAAGCCGAAGAGAAGATTGCTCTCACCAAAAACCGATTAGCATTACTTGAATCGACTGCTCTCGTTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 Val_tRNA-synt_C tRNA-synt_1

Protein Analysis

977

Amino Acids

111.31

Weight (kDa)

6.49

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1g PF09334 75 - 155 4.5e-06 tRNA synthetases class I (M)
tRNA-synt_1 PF00133 94 - 313 4.7e-79 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 234 - 329 9.2e-06 tRNA synthetases class I (M)
Anticodon_1 PF08264 367 - 511 8.7e-40 Anticodon-binding domain of tRNA ligase
Val_tRNA-synt_C PF10458 576 - 640 3.3e-13 Valyl tRNA synthetase tRNA binding arm
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1344
AasI GACNNNNNNGTC 1 cut(s) 181
AccB1I GGYRCC 1 cut(s) 974
AccBSI CCGCTC 2 cut(s) 26, 102
AccI GTMKAC 2 cut(s) 703, 1993
AccII CGCG 1 cut(s) 261
AclI AACGTT 1 cut(s) 2050
AclWI GGATC 7 cut(s) 263, 682, 1152, 1523, 1536, 1748, 1793
AcoI YGGCCR 2 cut(s) 1589, 2360
AcsI RAATTY 3 cut(s) 1957, 2108, 2410
AcyI GRCGYC 2 cut(s) 97, 156
AfaI GTAC 3 cut(s) 1421, 1579, 2775
AfiI CCNNNNNNNGG 2 cut(s) 1893, 2379
AhdI GACNNNNNGTC 2 cut(s) 924, 1866
AhlI ACTAGT 1 cut(s) 1234
AjiI CACGTC 1 cut(s) 934
AjnI CCWGG 3 cut(s) 113, 400, 2555
AjuI GAANNNNNNNTTGG 6 cut(s) 1120, 1152, 1464, 1496, 1898, 1930
AleI CACNNNNGTG 1 cut(s) 980
Alw21I GWGCWC 1 cut(s) 1564
Alw26I GTCTC 8 cut(s) 234, 699, 824, 1148, 1748, 2065, 2129, 2221
Alw44I GTGCAC 1 cut(s) 1560
AlwI GGATC 7 cut(s) 263, 682, 1152, 1523, 1536, 1748, 1793
AoxI GGCC 9 cut(s) 245, 1105, 1122, 1568, 1589, 1911, 2181, 2360, 2418
ApaLI GTGCAC 1 cut(s) 1560
ApeKI GCWGC 3 cut(s) 45, 313, 2855
ApoI RAATTY 3 cut(s) 1957, 2108, 2410
ArsI GACNNNNNNTTYG 2 cut(s) 375, 407
AspS9I GGNCC 3 cut(s) 246, 1123, 1873
AsuC2I CCSGG 2 cut(s) 827, 999
AsuHPI GGTGA 7 cut(s) 589, 697, 854, 1696, 2168, 2645, 2875
AvaII GGWCC 1 cut(s) 1873
AxyI CCTNAGG 3 cut(s) 65, 867, 2823
BaeGI GKGCMC 3 cut(s) 64, 327, 1564
BalI TGGCCA 1 cut(s) 2362
BamHI GGATCC 1 cut(s) 1528
BanI GGYRCC 1 cut(s) 974
BanII GRGCYC 1 cut(s) 1269
BauI CACGAG 1 cut(s) 2445
BbsI GAAGAC 2 cut(s) 388, 1034
Bbv12I GWGCWC 1 cut(s) 1564
BbvI GCAGC 3 cut(s) 32, 300, 2867
BccI CCATC 5 cut(s) 81, 1064, 1675, 1684, 2729
BceAI ACGGC 1 cut(s) 2711
BciT130I CCWGG 3 cut(s) 115, 402, 2557
BclI TGATCA 2 cut(s) 409, 1006
BcnI CCSGG 2 cut(s) 827, 999
BcoDI GTCTC 8 cut(s) 234, 699, 824, 1148, 1748, 2065, 2129, 2221
BcuI ACTAGT 1 cut(s) 1234
BfaI CTAG 5 cut(s) 699, 1235, 1856, 2027, 2561
BfmI CTRYAG 1 cut(s) 906
BglII AGATCT 2 cut(s) 1202, 1306
BisI GCNGC 9 cut(s) 46, 100, 135, 138, 141, 144, 290, 314, 2856
BlsI GCNGC 9 cut(s) 47, 101, 136, 139, 142, 145, 291, 315, 2857
BmcAI AGTACT 1 cut(s) 1579
Bme1390I CCNGG 5 cut(s) 115, 402, 827, 999, 2557
Bme18I GGWCC 1 cut(s) 1873
BmeRI GACNNNNNGTC 2 cut(s) 924, 1866
BmgBI CACGTC 1 cut(s) 934
BmgT120I GGNCC 3 cut(s) 246, 1123, 1873
BmiI GGNNCC 6 cut(s) 71, 399, 976, 1266, 1530, 1716
BmrFI CCNGG 5 cut(s) 115, 402, 827, 999, 2557
BmrI ACTGGG 2 cut(s) 1656, 1716
BmsI GCATC 8 cut(s) 464, 871, 1501, 1822, 1966, 2360, 2495, 2583
BmuI ACTGGG 2 cut(s) 1656, 1716
BoxI GACNNNNGTC 1 cut(s) 483
BpiI GAAGAC 2 cut(s) 388, 1034
BpmI CTGGAG 2 cut(s) 804, 1169
Bpu10I CCTNAGC 2 cut(s) 1270, 2235
BpuEI CTTGAG 2 cut(s) 365, 1476
BpuMI CCSGG 2 cut(s) 827, 999
BsaHI GRCGYC 2 cut(s) 97, 156
BsaI GGTCTC 3 cut(s) 699, 1748, 2065
BsaJI CCNNGG 6 cut(s) 113, 259, 401, 1782, 2357, 2378
BsaWI WCCGGW 1 cut(s) 749
BsaXI ACNNNNNCTCC 2 cut(s) 2575, 2605
Bsc4I CCNNNNNNNGG 2 cut(s) 1893, 2379
Bse118I RCCGGY 3 cut(s) 305, 977, 2467
Bse1I ACTGG 6 cut(s) 1355, 1412, 1651, 1711, 2174, 2597
Bse21I CCTNAGG 3 cut(s) 65, 867, 2823
BseBI CCWGG 3 cut(s) 115, 402, 2557
BseDI CCNNGG 6 cut(s) 113, 259, 401, 1782, 2357, 2378
BseGI GGATG 7 cut(s) 73, 862, 877, 1075, 1600, 1686, 1901
BseLI CCNNNNNNNGG 2 cut(s) 1893, 2379
BseNI ACTGG 6 cut(s) 1355, 1412, 1651, 1711, 2174, 2597
BseRI GAGGAG 1 cut(s) 1166
BseSI GKGCMC 3 cut(s) 64, 327, 1564
BseXI GCAGC 3 cut(s) 32, 300, 2867
BseYI CCCAGC 1 cut(s) 1964
BsgI GTGCAG 1 cut(s) 299
Bsh1236I CGCG 1 cut(s) 261
Bsh1285I CGRYCG 1 cut(s) 259
BshFI GGCC 9 cut(s) 247, 1107, 1124, 1570, 1591, 1913, 2183, 2362, 2420
BshNI GGYRCC 1 cut(s) 974
BsiEI CGRYCG 1 cut(s) 259
BsiHKAI GWGCWC 1 cut(s) 1564
BsiSI CCGG 7 cut(s) 306, 750, 827, 978, 999, 1592, 2468
BslFI GGGAC 6 cut(s) 418, 920, 981, 1413, 1723, 1873
BslI CCNNNNNNNGG 2 cut(s) 1893, 2379
BsmAI GTCTC 8 cut(s) 234, 699, 824, 1148, 1748, 2065, 2129, 2221
BsmFI GGGAC 6 cut(s) 418, 920, 981, 1413, 1723, 1873
BsmI GAATGC 4 cut(s) 453, 1939, 2446, 2608
BsnI GGCC 9 cut(s) 247, 1107, 1124, 1570, 1591, 1913, 2183, 2362, 2420
Bso31I GGTCTC 3 cut(s) 699, 1748, 2065
Bsp1286I GDGCHC 4 cut(s) 64, 327, 1269, 1564
Bsp19I CCATGG 1 cut(s) 2357
BspANI GGCC 9 cut(s) 247, 1107, 1124, 1570, 1591, 1913, 2183, 2362, 2420
BspFNI CGCG 1 cut(s) 261
BspHI TCATGA 2 cut(s) 649, 739
BspLI GGNNCC 6 cut(s) 71, 399, 976, 1266, 1530, 1716
BspPI GGATC 7 cut(s) 263, 682, 1152, 1523, 1536, 1748, 1793
BspQI GCTCTTC 2 cut(s) 55, 125
BspT107I GGYRCC 1 cut(s) 974
BspTNI GGTCTC 3 cut(s) 699, 1748, 2065
BsrBI CCGCTC 2 cut(s) 26, 102
BsrFI RCCGGY 3 cut(s) 305, 977, 2467
BsrI ACTGG 6 cut(s) 1355, 1412, 1651, 1711, 2174, 2597
BssAI RCCGGY 3 cut(s) 305, 977, 2467
BssECI CCNNGG 6 cut(s) 113, 259, 401, 1782, 2357, 2378
BssNAI GTATAC 1 cut(s) 1994
BssNI GRCGYC 2 cut(s) 97, 156
BssSI CACGAG 1 cut(s) 2445
BssT1I CCWWGG 3 cut(s) 1782, 2357, 2378
Bst1107I GTATAC 1 cut(s) 1994
Bst2BI CACGAG 1 cut(s) 2445
Bst2UI CCWGG 3 cut(s) 115, 402, 2557
Bst4CI ACNGT 4 cut(s) 127, 432, 1103, 1714
Bst6I CTCTTC 9 cut(s) 55, 125, 198, 486, 578, 1437, 1501, 2125, 2862
BstACI GRCGYC 2 cut(s) 97, 156
BstAPI GCANNNNNTGC 1 cut(s) 1960
BstC8I GCNNGC 7 cut(s) 311, 327, 453, 1244, 2098, 2185, 2319
BstDSI CCRYGG 2 cut(s) 259, 2357
BstF5I GGATG 7 cut(s) 73, 862, 877, 1075, 1600, 1686, 1901
BstFNI CGCG 1 cut(s) 261
BstMAI GTCTC 8 cut(s) 234, 699, 824, 1148, 1748, 2065, 2129, 2221
BstMCI CGRYCG 1 cut(s) 259
BstNI CCWGG 3 cut(s) 115, 402, 2557
BstPAI GACNNNNGTC 1 cut(s) 483
BstSCI CCNGG 5 cut(s) 113, 400, 825, 997, 2555
BstSFI CTRYAG 1 cut(s) 906
BstSLI GKGCMC 3 cut(s) 64, 327, 1564
BstUI CGCG 1 cut(s) 261
BstV1I GCAGC 3 cut(s) 32, 300, 2867
BstV2I GAAGAC 2 cut(s) 388, 1034
BstX2I RGATCY 7 cut(s) 674, 1144, 1202, 1306, 1528, 1753, 1798
BstXI CCANNNNNNTGG 3 cut(s) 393, 433, 2052
BstYI RGATCY 7 cut(s) 674, 1144, 1202, 1306, 1528, 1753, 1798
BstZ17I GTATAC 1 cut(s) 1994
Bsu36I CCTNAGG 3 cut(s) 65, 867, 2823
BsuRI GGCC 9 cut(s) 247, 1107, 1124, 1570, 1591, 1913, 2183, 2362, 2420
BtgI CCRYGG 2 cut(s) 259, 2357
BtgZI GCGATG 1 cut(s) 928
BtrI CACGTC 1 cut(s) 934
BtsCI GGATG 7 cut(s) 73, 862, 877, 1075, 1600, 1686, 1901
BtsI GCAGTG 1 cut(s) 1253
BtsIMutI CAGTG 6 cut(s) 1253, 1348, 1704, 1887, 2363, 2604
Cac8I GCNNGC 7 cut(s) 311, 327, 453, 1244, 2098, 2185, 2319
CciI TCATGA 2 cut(s) 649, 739
Cfr10I RCCGGY 3 cut(s) 305, 977, 2467
Cfr13I GGNCC 3 cut(s) 246, 1123, 1873
Cfr42I CCGCGG 1 cut(s) 262
CseI GACGC 3 cut(s) 105, 145, 1626
Csp6I GTAC 3 cut(s) 1420, 1578, 2774
CspCI CAANNNNNGTGG 2 cut(s) 1376, 1411
CviQI GTAC 3 cut(s) 1420, 1578, 2774
DrdI GACNNNNNNGTC 1 cut(s) 181
DriI GACNNNNNGTC 2 cut(s) 924, 1866
DseDI GACNNNNNNGTC 1 cut(s) 181
EaeI YGGCCR 2 cut(s) 1589, 2360
Eam1104I CTCTTC 9 cut(s) 55, 125, 198, 486, 578, 1437, 1501, 2125, 2862
Eam1105I GACNNNNNGTC 2 cut(s) 924, 1866
EarI CTCTTC 9 cut(s) 55, 125, 198, 486, 578, 1437, 1501, 2125, 2862
EciI GGCGGA 1 cut(s) 1157
Eco130I CCWWGG 3 cut(s) 1782, 2357, 2378
Eco147I AGGCCT 2 cut(s) 1913, 2420
Eco24I GRGCYC 1 cut(s) 1269
Eco31I GGTCTC 3 cut(s) 699, 1748, 2065
Eco32I GATATC 2 cut(s) 365, 2677
Eco47I GGWCC 1 cut(s) 1873
Eco81I CCTNAGG 3 cut(s) 65, 867, 2823
EcoO109I RGGNCCY 1 cut(s) 1873
EcoRII CCWGG 3 cut(s) 113, 400, 2555
EcoRV GATATC 2 cut(s) 365, 2677
EcoT14I CCWWGG 3 cut(s) 1782, 2357, 2378
EcoT38I GRGCYC 1 cut(s) 1269
ErhI CCWWGG 3 cut(s) 1782, 2357, 2378
FaqI GGGAC 6 cut(s) 418, 920, 981, 1413, 1723, 1873
FauNDI CATATG 1 cut(s) 922
FbaI TGATCA 2 cut(s) 409, 1006
FblI GTMKAC 2 cut(s) 703, 1993
Fnu4HI GCNGC 9 cut(s) 46, 100, 135, 138, 141, 144, 290, 314, 2856
FokI GGATG 7 cut(s) 60, 849, 884, 1082, 1607, 1693, 1888
FriOI GRGCYC 1 cut(s) 1269
Fsp4HI GCNGC 9 cut(s) 46, 100, 135, 138, 141, 144, 290, 314, 2856
FspBI CTAG 5 cut(s) 699, 1235, 1856, 2027, 2561
GluI GCNGC 9 cut(s) 46, 100, 135, 138, 141, 144, 290, 314, 2856
GsaI CCCAGC 1 cut(s) 1968
GsuI CTGGAG 2 cut(s) 804, 1169
HaeIII GGCC 9 cut(s) 247, 1107, 1124, 1570, 1591, 1913, 2183, 2362, 2420
HapII CCGG 7 cut(s) 306, 750, 827, 978, 999, 1592, 2468
HgaI GACGC 3 cut(s) 105, 145, 1626
Hin1I GRCGYC 2 cut(s) 97, 156
HincII GTYRAC 2 cut(s) 478, 1602
HindII GTYRAC 2 cut(s) 478, 1602
HindIII AAGCTT 1 cut(s) 1034
HinfI GANTC 9 cut(s) 4, 227, 746, 1598, 2084, 2588, 2640, 2739, 2909
HpaII CCGG 7 cut(s) 306, 750, 827, 978, 999, 1592, 2468
HphI GGTGA 7 cut(s) 589, 697, 854, 1696, 2168, 2645, 2875
Hpy166II GTNNAC 8 cut(s) 478, 704, 1562, 1602, 1704, 1887, 1994, 2620
Hpy188I TCNGA 9 cut(s) 460, 552, 721, 807, 1311, 1751, 2221, 2439, 2686
Hpy8I GTNNAC 8 cut(s) 478, 704, 1562, 1602, 1704, 1887, 1994, 2620
Hpy99I CGWCG 1 cut(s) 99
HpyAV CCTTC 8 cut(s) 28, 39, 196, 370, 455, 1924, 2384, 2630
HpyCH4III ACNGT 4 cut(s) 127, 432, 1103, 1714
HpyCH4IV ACGT 3 cut(s) 933, 1536, 2050
HpySE526I ACGT 3 cut(s) 933, 1536, 2050
Hsp92I GRCGYC 2 cut(s) 97, 156
Ksp22I TGATCA 2 cut(s) 409, 1006
KspI CCGCGG 1 cut(s) 262
LguI GCTCTTC 2 cut(s) 55, 125
LmnI GCTCC 7 cut(s) 31, 75, 107, 589, 1264, 2020, 2824
Lsp1109I GCAGC 3 cut(s) 32, 300, 2867
LweI GCATC 8 cut(s) 464, 871, 1501, 1822, 1966, 2360, 2495, 2583
MaeI CTAG 5 cut(s) 699, 1235, 1856, 2027, 2561
MaeII ACGT 3 cut(s) 933, 1536, 2050
MaeIII GTNAC 9 cut(s) 106, 301, 337, 1255, 2089, 2299, 2509, 2779, 2923
MbiI CCGCTC 2 cut(s) 26, 102
MflI RGATCY 7 cut(s) 674, 1144, 1202, 1306, 1528, 1753, 1798
MhlI GDGCHC 4 cut(s) 64, 327, 1269, 1564
MlsI TGGCCA 1 cut(s) 2362
MluNI TGGCCA 1 cut(s) 2362
MlyI GAGTC 5 cut(s) 236, 1607, 2078, 2634, 2733
MmeI TCCRAC 1 cut(s) 2716
Mox20I TGGCCA 1 cut(s) 2362
MscI TGGCCA 1 cut(s) 2362
MseI TTAA 8 cut(s) 468, 662, 768, 986, 1080, 1878, 2271, 2397
MslI CAYNNNNRTG 2 cut(s) 980, 2356
Msp20I TGGCCA 1 cut(s) 2362
MspA1I CMGCKG 2 cut(s) 219, 261
MspI CCGG 7 cut(s) 306, 750, 827, 978, 999, 1592, 2468
MspR9I CCNGG 5 cut(s) 115, 402, 827, 999, 2557
Mva1269I GAATGC 4 cut(s) 453, 1939, 2446, 2608
MvaI CCWGG 3 cut(s) 115, 402, 2557
MvnI CGCG 1 cut(s) 261
NciI CCSGG 2 cut(s) 827, 999
NcoI CCATGG 1 cut(s) 2357
NdeI CATATG 1 cut(s) 922
NlaIV GGNNCC 6 cut(s) 71, 399, 976, 1266, 1530, 1716
NmeAIII GCCGAG 2 cut(s) 172, 2728
NmuCI GTSAC 4 cut(s) 106, 301, 337, 2089
OliI CACNNNNGTG 1 cut(s) 980
PagI TCATGA 2 cut(s) 649, 739
PceI AGGCCT 2 cut(s) 1913, 2420
PciSI GCTCTTC 2 cut(s) 55, 125
PcsI WCGNNNNNNNCGW 2 cut(s) 333, 2443
PctI GAATGC 4 cut(s) 453, 1939, 2446, 2608
PfeI GAWTC 4 cut(s) 4, 746, 2588, 2909
PfoI TCCNGGA 1 cut(s) 997
PkrI GCNGC 9 cut(s) 47, 101, 136, 139, 142, 145, 291, 315, 2857
PleI GAGTC 5 cut(s) 235, 1606, 2078, 2634, 2733
PpsI GAGTC 5 cut(s) 235, 1606, 2078, 2634, 2733
PpuMI RGGWCCY 1 cut(s) 1873
PshAI GACNNNNGTC 1 cut(s) 483
PsiI TTATAA 1 cut(s) 1344
Psp1406I AACGTT 1 cut(s) 2050
Psp5II RGGWCCY 1 cut(s) 1873
Psp6I CCWGG 3 cut(s) 113, 400, 2555
PspFI CCCAGC 1 cut(s) 1964
PspGI CCWGG 3 cut(s) 113, 400, 2555
PspN4I GGNNCC 6 cut(s) 71, 399, 976, 1266, 1530, 1716
PspPI GGNCC 3 cut(s) 246, 1123, 1873
PspPPI RGGWCCY 1 cut(s) 1873
PsuI RGATCY 7 cut(s) 674, 1144, 1202, 1306, 1528, 1753, 1798
PvuII CAGCTG 1 cut(s) 219
RsaI GTAC 3 cut(s) 1421, 1579, 2775
RsaNI GTAC 3 cut(s) 1420, 1578, 2774
RseI CAYNNNNRTG 2 cut(s) 980, 2356
SacII CCGCGG 1 cut(s) 262
SapI GCTCTTC 2 cut(s) 55, 125
SaqAI TTAA 8 cut(s) 468, 662, 768, 986, 1080, 1878, 2271, 2397
SatI GCNGC 9 cut(s) 46, 100, 135, 138, 141, 144, 290, 314, 2856
Sau96I GGNCC 3 cut(s) 246, 1123, 1873
ScaI AGTACT 1 cut(s) 1579
SchI GAGTC 5 cut(s) 236, 1607, 2078, 2634, 2733
ScrFI CCNGG 5 cut(s) 115, 402, 827, 999, 2557
SduI GDGCHC 4 cut(s) 64, 327, 1269, 1564
SfaNI GCATC 8 cut(s) 464, 871, 1501, 1822, 1966, 2360, 2495, 2583
SfcI CTRYAG 1 cut(s) 906
Sfr303I CCGCGG 1 cut(s) 262
SgrAI CRCCGGYG 1 cut(s) 977
SgrBI CCGCGG 1 cut(s) 262
SinI GGWCC 1 cut(s) 1873
SmiMI CAYNNNNRTG 2 cut(s) 980, 2356
SmlI CTYRAG 2 cut(s) 344, 1491
SmoI CTYRAG 2 cut(s) 344, 1491
SpeI ACTAGT 1 cut(s) 1234
SseBI AGGCCT 2 cut(s) 1913, 2420
SspI AATATT 1 cut(s) 2456
SspMI CTAG 5 cut(s) 699, 1235, 1856, 2027, 2561
StuI AGGCCT 2 cut(s) 1913, 2420
StyD4I CCNGG 5 cut(s) 113, 400, 825, 997, 2555
StyI CCWWGG 3 cut(s) 1782, 2357, 2378
TaaI ACNGT 4 cut(s) 127, 432, 1103, 1714
TaiI ACGT 3 cut(s) 936, 1539, 2053
TaqI TCGA 9 cut(s) 189, 255, 487, 619, 2063, 2082, 2332, 2643, 2912
TatI WGTACW 1 cut(s) 1577
TauI GCSGC 6 cut(s) 102, 137, 140, 143, 146, 292
TfiI GAWTC 4 cut(s) 4, 746, 2588, 2909
Tru1I TTAA 8 cut(s) 468, 662, 768, 986, 1080, 1878, 2271, 2397
Tru9I TTAA 8 cut(s) 468, 662, 768, 986, 1080, 1878, 2271, 2397
TscAI CASTG 6 cut(s) 1253, 1355, 1711, 1894, 2370, 2604
TseFI GTSAC 4 cut(s) 106, 301, 337, 2089
TseI GCWGC 3 cut(s) 45, 313, 2855
Tsp45I GTSAC 4 cut(s) 106, 301, 337, 2089
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 6 cut(s) 1253, 1355, 1711, 1894, 2370, 2604
VneI GTGCAC 1 cut(s) 1560
VpaK11BI GGWCC 1 cut(s) 1873
XapI RAATTY 3 cut(s) 1957, 2108, 2410
XbaI TCTAGA 1 cut(s) 2026
XcmI CCANNNNNNNNNTGG 1 cut(s) 2627
XmiI GTMKAC 2 cut(s) 703, 1993
XspI CTAG 5 cut(s) 699, 1235, 1856, 2027, 2561
ZrmI AGTACT 1 cut(s) 1579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.