MD09G1264300.v1.1

valine--tRNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004429
Physical Location & Seq
Reverse (-)
33725599 .. 33726781
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1264300.v1.1.491

Sequence Viewer

Length: 309 bp
ATGTATTTTACTTCAGGGAACGCGGATCAATCAGTCCACCTTGTAGCTGGTGAAGGACTAGAGGCATATCTCCCTCTTGCTGACATGATTGATATTACTGCCGAAATCCAACGCCTATCTAAGAGACTTTCCAAGATGCAGGCGGAATATGATGGACTTAAAGCTCGGCTCAGCTCTCCTAAATTTGTAGAGAAAGCTCCTGAGGATATTGTCCGTGGAGTGCAAGAAAAGGCGGCAGAAACAGAAGAGAAGCTTACTCTCACCAAAAACCGTTTAGCATTACTCGAATCAAGTGTTCTGGTCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 Val_tRNA-synt_C tRNA-synt_1

Protein Analysis

103

Amino Acids

11.27

Weight (kDa)

5.17

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Val_tRNA-synt_C PF10458 31 - 95 7.6e-16 Valyl tRNA synthetase tRNA binding arm
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 23
AciI CCGC 3 cut(s) 23, 143, 233
AclWI GGATC 1 cut(s) 33
AcsI RAATTY 1 cut(s) 182
AluBI AGCT 5 cut(s) 47, 164, 174, 197, 253
AluI AGCT 5 cut(s) 47, 164, 174, 197, 253
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 33
ApoI RAATTY 1 cut(s) 182
AsuHPI GGTGA 2 cut(s) 62, 253
AxyI CCTNAGG 1 cut(s) 201
BccI CCATC 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 118
BfaI CTAG 1 cut(s) 59
BisI GCNGC 1 cut(s) 234
BlpI GCTNAGC 1 cut(s) 170
BlsI GCNGC 1 cut(s) 235
BmsI GCATC 1 cut(s) 126
Bpu1102I GCTNAGC 1 cut(s) 170
BsaJI CCNNGG 1 cut(s) 214
Bse21I CCTNAGG 1 cut(s) 201
BseDI CCNNGG 1 cut(s) 214
BseMII CTCAG 2 cut(s) 184, 192
Bsh1236I CGCG 1 cut(s) 23
BsmAI GTCTC 1 cut(s) 118
Bsp143I GATC 1 cut(s) 25
Bsp1720I GCTNAGC 1 cut(s) 170
BspACI CCGC 3 cut(s) 23, 143, 233
BspCNI CTCAG 2 cut(s) 183, 193
BspFNI CGCG 1 cut(s) 23
BspPI GGATC 1 cut(s) 33
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 1 cut(s) 25
Bst4CI ACNGT 1 cut(s) 272
Bst6I CTCTTC 1 cut(s) 240
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 3 cut(s) 120, 170, 201
BstDSI CCRYGG 1 cut(s) 214
BstFNI CGCG 1 cut(s) 23
BstKTI GATC 1 cut(s) 28
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 1 cut(s) 25
BstUI CGCG 1 cut(s) 23
Bsu36I CCTNAGG 1 cut(s) 201
BtgI CCRYGG 1 cut(s) 214
Cac8I GCNNGC 1 cut(s) 141
CviAII CATG 1 cut(s) 85
CviJI RGCY 6 cut(s) 47, 164, 169, 174, 197, 253
CviKI_1 RGCY 6 cut(s) 47, 164, 169, 174, 197, 253
DdeI CTNAG 3 cut(s) 120, 170, 201
DpnI GATC 1 cut(s) 27
DpnII GATC 1 cut(s) 25
Eam1104I CTCTTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 240
EciI GGCGGA 1 cut(s) 158
Eco81I CCTNAGG 1 cut(s) 201
FaeI CATG 1 cut(s) 88
FaiI YATR 4 cut(s) 67, 86, 150, 307
FalI AAGNNNNNCTT 2 cut(s) 237, 269
FatI CATG 1 cut(s) 84
Fnu4HI GCNGC 1 cut(s) 234
Fsp4HI GCNGC 1 cut(s) 234
FspBI CTAG 1 cut(s) 59
GluI GCNGC 1 cut(s) 234
Hin1II CATG 1 cut(s) 88
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 1 cut(s) 287
HphI GGTGA 2 cut(s) 62, 253
Hpy166II GTNNAC 1 cut(s) 37
Hpy188III TCNNGA 1 cut(s) 200
Hpy8I GTNNAC 1 cut(s) 37
HpyAV CCTTC 1 cut(s) 47
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4V TGCA 2 cut(s) 139, 223
HpyF3I CTNAG 3 cut(s) 120, 170, 201
Hsp92II CATG 1 cut(s) 88
Kzo9I GATC 1 cut(s) 25
LmnI GCTCC 1 cut(s) 202
LpnPI CCDG 4 cut(s) 33, 125, 213, 284
LweI GCATC 1 cut(s) 126
MaeI CTAG 1 cut(s) 59
MaeIII GTNAC 1 cut(s) 301
MalI GATC 1 cut(s) 27
MboI GATC 1 cut(s) 25
MboII GAAGA 1 cut(s) 257
MluCI AATT 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 3 cut(s) 55, 84, 196
MseI TTAA 1 cut(s) 159
MvnI CGCG 1 cut(s) 23
NdeII GATC 1 cut(s) 25
NlaIII CATG 1 cut(s) 88
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 1 cut(s) 301
PfeI GAWTC 1 cut(s) 287
PkrI GCNGC 1 cut(s) 235
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 1 cut(s) 25
SetI ASST 6 cut(s) 42, 49, 166, 176, 199, 255
SfaNI GCATC 1 cut(s) 126
Sse9I AATT 1 cut(s) 182
SsiI CCGC 3 cut(s) 23, 143, 233
SspMI CTAG 1 cut(s) 59
TaaI ACNGT 1 cut(s) 272
TaqI TCGA 1 cut(s) 285
TasI AATT 1 cut(s) 182
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 1 cut(s) 287
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TseFI GTSAC 1 cut(s) 301
Tsp45I GTSAC 1 cut(s) 301
TspGWI ACGGA 1 cut(s) 203
XapI RAATTY 1 cut(s) 182
XcmI CCANNNNNNNNNTGG 1 cut(s) 44
XspI CTAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.