MD17G1259800.v1.1

valine--tRNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004429
Physical Location & Seq
Reverse (-)
32048843 .. 32059289
10447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1259800.v1.1.491

Sequence Viewer

Length: 1701 bp
ATGAGGGAAAAGAATGGGAAGGCTGAACAGCACAAACGAAATGCGTGGCCAGAAAGGGTAAATCCGCCACTTCTCTGTATAGAAACGGAAAAAATAAGAACTCCAAATAGTAAGTGGAGGACAATTTCGGTAGTTCACTGTTCACGAACAGTGAAAAACAGGTCCTACATGGTGAATTGGTCTCCTAGTCTACAGACTGCCGTTTCTGACTTGGAAGTAGAATATCATGAAGAATCCGGAACTCTTTATTATCTTAAGTATCGTGTTGCTGGAGATCTTATGTTAATCCTCTCAGCTAGAGTTGGCTTTTTCTCTTCCGGGTTCTGGTTGGGGTGGTCCGACTATCTGACAATAGCAACAACACGTCCGGAGACATTATTTGGTGATGTAGCTATTGCTGTGCATCCTGAGGATGATCGATATTCTAAATATATTAATAGAATGGCTATAGTGCCTTTGACATATGGCCGTCACATCCCTATCATCGCTGACAAGCATGTTGAGAAAGACTTTGGCACTGGCGTGCTAAAGATAAGCCCTGGACATGATCATAATGATTACAATCTTGCAAGAAAGCTTGGTCTTCCAATACTTAATGTCATGAACAAGGATGGAACGCTAAACAAGGTTGCCGGACTGTACTGTGGCCTTGATCGGTTTGAGGCCCGGAAGAAACTTTGGGCAGATCTGGAGGAGACTGGTTTAGCTTGGTTTGTAACGATGGAGCCCTTAGCTGAGAAAGCCCTTAGTGCTGTTGAAAAAGGAGACCTGAAAATTATACCTGAAAGATTTGAAAAGGTATGCGCTATTTGGCCTCATATTGACTCTTCTGGGACACCAAGTAGTTTTCCTCTCTTTTCAGTTGGAGCCTTCAAGACTCTGTACCGAGCACGAGTGCTGATATCTGTTCTGTGCATTATTTACAATCACTGGCTATCAAATATTAAGGATTGGTGCATAAGCAGACAACTGTGGTGGGGTCATCGAATACCAGTTTGGTACATTGTGGGTAAAGATTGTGAAGAAGAATATATAGTTGCAAGGAGTGAGGATGAAGCTCTTGGGAAAGCTCAAGAAAAGTATGGAAAAGATGCAAAAATATATCAGGATCCGGATGTTCTTGACACATGGTTTTCGAGTGCACTGTGGCTTTTCAGTACTCTTGGATGGCCAGATGAGTCAGTTGAGGATGGCCAGATGAGTCAGCATGACATATTGTTCTTTTGGGTTGCGAGGATGGTCATGATGGGAATTGAGTTCACAGGGACAGTGCCATTTAAATACATTTATCTACACGGTCTCATCAGGGATTCACAAGGTCGAAGGATGTCGAAAACATTGGGGAATGTAATAGATCCTTTAGATACAATCAAGGAATATGGCACAGATGCTTTAAGATTTACAATTGCTCTAGGGACTGCTGGCCAGGACCTTAATTTATCCACCGAGAGGTTGACATCCAACAAGGCCTTCACTAATAAACTATGGAATGCTGGAAAATTTGTATTGCACAATTTACCCAGTCAAAACGATGCATCAACTTGGGAAAATATACTTTCTTATAAGATTTCAAAACTTCATTTGCTTATTGACACAGTCGCCACAAGCTATGACAAATTTTTCTTTGGAGATGTAGGAAGAGAGACATATGATTTCTTTTGGGGAGATTTTGCTGATTGGTATGTTTTGAGTTTCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 Val_tRNA-synt_C tRNA-synt_1

Protein Analysis

567

Amino Acids

64.98

Weight (kDa)

6.37

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 34 - 271 7e-10 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1_2 PF13603 146 - 206 1.4e-07 Leucyl-tRNA synthetase, editing domain
tRNA-synt_1 PF00133 308 - 481 2.5e-57 tRNA synthetases class I (I, L, M and V)
Anticodon_1 PF08264 523 - 562 1.9e-06 Anticodon-binding domain of tRNA ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1563
AccI GTMKAC 1 cut(s) 190
AccIII TCCGGA 3 cut(s) 236, 367, 1111
AciI CCGC 1 cut(s) 65
AclWI GGATC 3 cut(s) 1103, 1116, 1349
AcoI YGGCCR 5 cut(s) 47, 466, 1169, 1192, 1423
AcsI RAATTY 2 cut(s) 1499, 1616
AfaI GTAC 4 cut(s) 641, 884, 1001, 1159
AfiI CCNNNNNNNGG 2 cut(s) 324, 1449
AflII CTTAAG 1 cut(s) 254
AflIII ACRYGT 1 cut(s) 362
AgsI TTSAA 4 cut(s) 758, 794, 874, 1572
AjiI CACGTC 1 cut(s) 365
AjnI CCWGG 2 cut(s) 538, 1425
AjuI GAANNNNNNNTTGG 4 cut(s) 979, 1011, 1454, 1486
AleI CACNNNNGTG 1 cut(s) 521
AluBI AGCT 8 cut(s) 296, 392, 577, 707, 734, 1058, 1070, 1608
AluI AGCT 8 cut(s) 296, 392, 577, 707, 734, 1058, 1070, 1608
Alw21I GWGCWC 2 cut(s) 892, 1144
Alw26I GTCTC 6 cut(s) 186, 365, 689, 759, 1304, 1637
Alw44I GTGCAC 1 cut(s) 1140
AlwI GGATC 3 cut(s) 1103, 1116, 1349
Aor13HI TCCGGA 3 cut(s) 236, 367, 1111
AoxI GGCC 9 cut(s) 47, 466, 646, 663, 812, 1169, 1192, 1423, 1467
ApaLI GTGCAC 1 cut(s) 1140
ApoI RAATTY 2 cut(s) 1499, 1616
AseI ATTAAT 1 cut(s) 435
AspLEI GCGC 1 cut(s) 806
AspS9I GGNCC 4 cut(s) 162, 336, 664, 1429
AsuC2I CCSGG 2 cut(s) 319, 667
AsuHPI GGTGA 2 cut(s) 184, 395
AvaII GGWCC 3 cut(s) 162, 336, 1429
AxyI CCTNAGG 1 cut(s) 408
BaeGI GKGCMC 1 cut(s) 1144
BalI TGGCCA 4 cut(s) 49, 1171, 1194, 1425
BamHI GGATCC 1 cut(s) 1108
BanII GRGCYC 1 cut(s) 729
BauI CACGAG 1 cut(s) 891
BbsI GAAGAC 1 cut(s) 575
Bbv12I GWGCWC 2 cut(s) 892, 1144
BccI CCATC 6 cut(s) 605, 715, 1161, 1184, 1231, 1240
BceAI ACGGC 2 cut(s) 185, 453
BciT130I CCWGG 2 cut(s) 540, 1427
BclI TGATCA 1 cut(s) 547
BcnI CCSGG 2 cut(s) 319, 667
BcoDI GTCTC 6 cut(s) 186, 365, 689, 759, 1304, 1637
BfaI CTAG 3 cut(s) 186, 297, 1412
BfmI CTRYAG 2 cut(s) 191, 447
BfrI CTTAAG 1 cut(s) 254
BglII AGATCT 2 cut(s) 274, 685
BmcAI AGTACT 1 cut(s) 1159
Bme1390I CCNGG 4 cut(s) 319, 540, 667, 1427
Bme18I GGWCC 3 cut(s) 162, 336, 1429
BmgBI CACGTC 1 cut(s) 365
BmgT120I GGNCC 4 cut(s) 162, 336, 664, 1429
BmiI GGNNCC 3 cut(s) 726, 868, 1110
BmrFI CCNGG 4 cut(s) 319, 540, 667, 1427
BmrI ACTGGG 1 cut(s) 1515
BmsI GCATC 5 cut(s) 412, 1081, 1378, 1522, 1544
BmuI ACTGGG 1 cut(s) 1515
BpiI GAAGAC 1 cut(s) 575
BpmI CTGGAG 2 cut(s) 291, 710
Bpu10I CCTNAGC 1 cut(s) 730
BpuEI CTTGAG 1 cut(s) 1056
BpuMI CCSGG 2 cut(s) 319, 667
Bsa29I ATCGAT 1 cut(s) 418
BsaBI GATNNNNATC 1 cut(s) 561
BsaI GGTCTC 3 cut(s) 186, 759, 1304
BsaJI CCNNGG 1 cut(s) 538
BsaWI WCCGGW 3 cut(s) 236, 367, 1111
Bsc4I CCNNNNNNNGG 2 cut(s) 324, 1449
Bse1I ACTGG 5 cut(s) 523, 703, 935, 992, 1521
Bse21I CCTNAGG 1 cut(s) 408
Bse8I GATNNNNATC 1 cut(s) 561
BseAI TCCGGA 3 cut(s) 236, 367, 1111
BseBI CCWGG 2 cut(s) 540, 1427
BseCI ATCGAT 1 cut(s) 418
BseDI CCNNGG 1 cut(s) 538
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 2 cut(s) 324, 1449
BseMII CTCAG 3 cut(s) 306, 399, 726
BseNI ACTGG 5 cut(s) 523, 703, 935, 992, 1521
BseRI GAGGAG 1 cut(s) 707
BseSI GKGCMC 1 cut(s) 1144
BshFI GGCC 9 cut(s) 49, 468, 648, 665, 814, 1171, 1194, 1425, 1469
BshVI ATCGAT 1 cut(s) 418
BsiHKAI GWGCWC 2 cut(s) 892, 1144
BsiSI CCGG 6 cut(s) 237, 318, 368, 633, 667, 1112
BslFI GGGAC 3 cut(s) 847, 1279, 1429
BslI CCNNNNNNNGG 2 cut(s) 324, 1449
BsmAI GTCTC 6 cut(s) 186, 365, 689, 759, 1304, 1637
BsmFI GGGAC 3 cut(s) 847, 1279, 1429
BsmI GAATGC 1 cut(s) 1495
BsnI GGCC 9 cut(s) 49, 468, 648, 665, 814, 1171, 1194, 1425, 1469
Bso31I GGTCTC 3 cut(s) 186, 759, 1304
Bsp1286I GDGCHC 3 cut(s) 729, 892, 1144
Bsp13I TCCGGA 3 cut(s) 236, 367, 1111
Bsp143I GATC 7 cut(s) 274, 415, 547, 652, 685, 1108, 1354
BspACI CCGC 1 cut(s) 65
BspANI GGCC 9 cut(s) 49, 468, 648, 665, 814, 1171, 1194, 1425, 1469
BspCNI CTCAG 3 cut(s) 305, 400, 727
BspDI ATCGAT 1 cut(s) 418
BspEI TCCGGA 3 cut(s) 236, 367, 1111
BspHI TCATGA 3 cut(s) 226, 600, 1242
BspLI GGNNCC 3 cut(s) 726, 868, 1110
BspPI GGATC 3 cut(s) 1103, 1116, 1349
BspTI CTTAAG 1 cut(s) 254
BspTNI GGTCTC 3 cut(s) 186, 759, 1304
BsrI ACTGG 5 cut(s) 523, 703, 935, 992, 1521
BssECI CCNNGG 1 cut(s) 538
BssMI GATC 7 cut(s) 274, 415, 547, 652, 685, 1108, 1354
BssSI CACGAG 1 cut(s) 891
Bst2BI CACGAG 1 cut(s) 891
Bst2UI CCWGG 2 cut(s) 540, 1427
Bst4CI ACNGT 9 cut(s) 140, 151, 639, 644, 972, 1146, 1270, 1298, 1597
Bst6I CTCTTC 3 cut(s) 319, 832, 1633
BstAFI CTTAAG 1 cut(s) 254
BstC8I GCNNGC 2 cut(s) 524, 1423
BstDEI CTNAG 5 cut(s) 292, 408, 730, 735, 746
BstHHI GCGC 1 cut(s) 806
BstKTI GATC 7 cut(s) 277, 418, 550, 655, 688, 1111, 1357
BstMAI GTCTC 6 cut(s) 186, 365, 689, 759, 1304, 1637
BstMBI GATC 7 cut(s) 274, 415, 547, 652, 685, 1108, 1354
BstMWI GCNNNNNNNGC 2 cut(s) 740, 749
BstNI CCWGG 2 cut(s) 540, 1427
BstNSI RCATGY 1 cut(s) 500
BstSCI CCNGG 4 cut(s) 317, 538, 665, 1425
BstSFI CTRYAG 2 cut(s) 191, 447
BstSLI GKGCMC 1 cut(s) 1144
BstV2I GAAGAC 1 cut(s) 575
BstX2I RGATCY 4 cut(s) 274, 685, 1108, 1354
BstYI RGATCY 4 cut(s) 274, 685, 1108, 1354
Bsu15I ATCGAT 1 cut(s) 418
Bsu36I CCTNAGG 1 cut(s) 408
BsuRI GGCC 9 cut(s) 49, 468, 648, 665, 814, 1171, 1194, 1425, 1469
BsuTUI ATCGAT 1 cut(s) 418
BtgZI GCGATG 1 cut(s) 469
BtrI CACGTC 1 cut(s) 365
BtsIMutI CAGTG 6 cut(s) 136, 156, 516, 928, 1142, 1275
Cac8I GCNNGC 2 cut(s) 524, 1423
CciI TCATGA 3 cut(s) 226, 600, 1242
CfoI GCGC 1 cut(s) 806
Cfr13I GGNCC 4 cut(s) 162, 336, 664, 1429
ClaI ATCGAT 1 cut(s) 418
Csp6I GTAC 4 cut(s) 640, 883, 1000, 1158
CspCI CAANNNNNGTGG 2 cut(s) 956, 991
CviAII CATG 8 cut(s) 169, 227, 497, 545, 601, 1128, 1208, 1243
CviQI GTAC 4 cut(s) 640, 883, 1000, 1158
DdeI CTNAG 5 cut(s) 292, 408, 730, 735, 746
DpnI GATC 7 cut(s) 276, 417, 549, 654, 687, 1110, 1356
DpnII GATC 7 cut(s) 274, 415, 547, 652, 685, 1108, 1354
DraI TTTAAA 1 cut(s) 1279
EaeI YGGCCR 5 cut(s) 47, 466, 1169, 1192, 1423
Eam1104I CTCTTC 3 cut(s) 319, 832, 1633
EarI CTCTTC 3 cut(s) 319, 832, 1633
EciI GGCGGA 1 cut(s) 54
Eco147I AGGCCT 1 cut(s) 1469
Eco24I GRGCYC 1 cut(s) 729
Eco31I GGTCTC 3 cut(s) 186, 759, 1304
Eco32I GATATC 1 cut(s) 903
Eco47I GGWCC 3 cut(s) 162, 336, 1429
Eco81I CCTNAGG 1 cut(s) 408
EcoO109I RGGNCCY 2 cut(s) 162, 1429
EcoRII CCWGG 2 cut(s) 538, 1425
EcoRV GATATC 1 cut(s) 903
EcoT22I ATGCAT 1 cut(s) 1537
EcoT38I GRGCYC 1 cut(s) 729
FaeI CATG 8 cut(s) 172, 230, 500, 548, 604, 1131, 1211, 1246
FaqI GGGAC 3 cut(s) 847, 1279, 1429
FatI CATG 8 cut(s) 168, 226, 496, 544, 600, 1127, 1207, 1242
FauNDI CATATG 2 cut(s) 463, 1648
FbaI TGATCA 1 cut(s) 547
FblI GTMKAC 1 cut(s) 190
FriOI GRGCYC 1 cut(s) 729
FspBI CTAG 3 cut(s) 186, 297, 1412
GlaI GCGC 1 cut(s) 805
GsuI CTGGAG 2 cut(s) 291, 710
HaeIII GGCC 9 cut(s) 49, 468, 648, 665, 814, 1171, 1194, 1425, 1469
HapII CCGG 6 cut(s) 237, 318, 368, 633, 667, 1112
HhaI GCGC 1 cut(s) 806
Hin1II CATG 8 cut(s) 172, 230, 500, 548, 604, 1131, 1211, 1246
Hin6I GCGC 1 cut(s) 804
HinP1I GCGC 1 cut(s) 804
HincII GTYRAC 1 cut(s) 1455
HindII GTYRAC 1 cut(s) 1455
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 6 cut(s) 233, 824, 877, 1178, 1201, 1310
HpaII CCGG 6 cut(s) 237, 318, 368, 633, 667, 1112
HphI GGTGA 2 cut(s) 184, 395
Hpy166II GTNNAC 6 cut(s) 136, 143, 191, 1142, 1260, 1455
Hpy188I TCNGA 3 cut(s) 208, 340, 348
Hpy8I GTNNAC 6 cut(s) 136, 143, 191, 1142, 1260, 1455
HpyAV CCTTC 4 cut(s) 13, 880, 1317, 1480
HpyCH4III ACNGT 9 cut(s) 140, 151, 639, 644, 972, 1146, 1270, 1298, 1597
HpyCH4IV ACGT 1 cut(s) 364
HpyCH4V TGCA 9 cut(s) 403, 569, 915, 957, 1040, 1094, 1142, 1510, 1535
HpyF10VI GCNNNNNNNGC 2 cut(s) 740, 749
HpyF3I CTNAG 5 cut(s) 292, 408, 730, 735, 746
HpySE526I ACGT 1 cut(s) 364
Hsp92II CATG 8 cut(s) 172, 230, 500, 548, 604, 1131, 1211, 1246
HspAI GCGC 1 cut(s) 804
Kpn2I TCCGGA 3 cut(s) 236, 367, 1111
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 7 cut(s) 274, 415, 547, 652, 685, 1108, 1354
LmnI GCTCC 2 cut(s) 724, 866
LweI GCATC 5 cut(s) 412, 1081, 1378, 1522, 1544
MaeI CTAG 3 cut(s) 186, 297, 1412
MaeII ACGT 1 cut(s) 364
MaeIII GTNAC 2 cut(s) 470, 715
MalI GATC 7 cut(s) 276, 417, 549, 654, 687, 1110, 1356
MboI GATC 7 cut(s) 274, 415, 547, 652, 685, 1108, 1354
MboII GAAGA 8 cut(s) 242, 306, 575, 682, 819, 1034, 1037, 1650
MfeI CAATTG 1 cut(s) 1404
MflI RGATCY 4 cut(s) 274, 685, 1108, 1354
MhlI GDGCHC 3 cut(s) 729, 892, 1144
MlsI TGGCCA 4 cut(s) 49, 1171, 1194, 1425
MluCI AATT 9 cut(s) 123, 175, 774, 1251, 1404, 1435, 1499, 1513, 1616
MluNI TGGCCA 4 cut(s) 49, 1171, 1194, 1425
MlyI GAGTC 4 cut(s) 818, 871, 1187, 1210
MmeI TCCRAC 3 cut(s) 363, 844, 1485
Mox20I TGGCCA 4 cut(s) 49, 1171, 1194, 1425
Mph1103I ATGCAT 1 cut(s) 1537
MroI TCCGGA 3 cut(s) 236, 367, 1111
MscI TGGCCA 4 cut(s) 49, 1171, 1194, 1425
MseI TTAA 9 cut(s) 255, 284, 435, 594, 945, 1278, 1394, 1434, 1699
MslI CAYNNNNRTG 1 cut(s) 521
Msp20I TGGCCA 4 cut(s) 49, 1171, 1194, 1425
MspCI CTTAAG 1 cut(s) 254
MspI CCGG 6 cut(s) 237, 318, 368, 633, 667, 1112
MspR9I CCNGG 4 cut(s) 319, 540, 667, 1427
MunI CAATTG 1 cut(s) 1404
Mva1269I GAATGC 1 cut(s) 1495
MvaI CCWGG 2 cut(s) 540, 1427
MwoI GCNNNNNNNGC 2 cut(s) 740, 749
NciI CCSGG 2 cut(s) 319, 667
NdeI CATATG 2 cut(s) 463, 1648
NdeII GATC 7 cut(s) 274, 415, 547, 652, 685, 1108, 1354
NlaIII CATG 8 cut(s) 172, 230, 500, 548, 604, 1131, 1211, 1246
NlaIV GGNNCC 3 cut(s) 726, 868, 1110
NmuCI GTSAC 1 cut(s) 470
NsiI ATGCAT 1 cut(s) 1537
NspI RCATGY 1 cut(s) 500
OliI CACNNNNGTG 1 cut(s) 521
PagI TCATGA 3 cut(s) 226, 600, 1242
PceI AGGCCT 1 cut(s) 1469
PctI GAATGC 1 cut(s) 1495
PfeI GAWTC 2 cut(s) 233, 1310
PflFI GACNNNGTC 1 cut(s) 1595
PleI GAGTC 4 cut(s) 818, 871, 1186, 1209
PpsI GAGTC 4 cut(s) 818, 871, 1186, 1209
PpuMI RGGWCCY 2 cut(s) 162, 1429
PshBI ATTAAT 1 cut(s) 435
PsiI TTATAA 1 cut(s) 1563
Psp5II RGGWCCY 2 cut(s) 162, 1429
Psp6I CCWGG 2 cut(s) 538, 1425
PspGI CCWGG 2 cut(s) 538, 1425
PspN4I GGNNCC 3 cut(s) 726, 868, 1110
PspPI GGNCC 4 cut(s) 162, 336, 664, 1429
PspPPI RGGWCCY 2 cut(s) 162, 1429
PsuI RGATCY 4 cut(s) 274, 685, 1108, 1354
PsyI GACNNNGTC 1 cut(s) 1595
RsaI GTAC 4 cut(s) 641, 884, 1001, 1159
RsaNI GTAC 4 cut(s) 640, 883, 1000, 1158
RseI CAYNNNNRTG 1 cut(s) 521
SaqAI TTAA 9 cut(s) 255, 284, 435, 594, 945, 1278, 1394, 1434, 1699
Sau3AI GATC 7 cut(s) 274, 415, 547, 652, 685, 1108, 1354
Sau96I GGNCC 4 cut(s) 162, 336, 664, 1429
ScaI AGTACT 1 cut(s) 1159
SchI GAGTC 4 cut(s) 818, 871, 1187, 1210
ScrFI CCNGG 4 cut(s) 319, 540, 667, 1427
SduI GDGCHC 3 cut(s) 729, 892, 1144
SfaNI GCATC 5 cut(s) 412, 1081, 1378, 1522, 1544
SfcI CTRYAG 2 cut(s) 191, 447
SinI GGWCC 3 cut(s) 162, 336, 1429
SmiI ATTTAAAT 1 cut(s) 1279
SmiMI CAYNNNNRTG 1 cut(s) 521
SmlI CTYRAG 2 cut(s) 254, 1071
SmoI CTYRAG 2 cut(s) 254, 1071
Sse9I AATT 9 cut(s) 123, 175, 774, 1251, 1404, 1435, 1499, 1513, 1616
SseBI AGGCCT 1 cut(s) 1469
SsiI CCGC 1 cut(s) 65
SspI AATATT 1 cut(s) 943
SspMI CTAG 3 cut(s) 186, 297, 1412
StuI AGGCCT 1 cut(s) 1469
StyD4I CCNGG 4 cut(s) 317, 538, 665, 1425
SwaI ATTTAAAT 1 cut(s) 1279
TaaI ACNGT 9 cut(s) 140, 151, 639, 644, 972, 1146, 1270, 1298, 1597
TaiI ACGT 1 cut(s) 367
TaqI TCGA 5 cut(s) 418, 985, 1136, 1321, 1331
TasI AATT 9 cut(s) 123, 175, 774, 1251, 1404, 1435, 1499, 1513, 1616
TatI WGTACW 2 cut(s) 639, 1157
TfiI GAWTC 2 cut(s) 233, 1310
Tru1I TTAA 9 cut(s) 255, 284, 435, 594, 945, 1278, 1394, 1434, 1699
Tru9I TTAA 9 cut(s) 255, 284, 435, 594, 945, 1278, 1394, 1434, 1699
TscAI CASTG 6 cut(s) 143, 156, 523, 935, 1149, 1275
TseFI GTSAC 1 cut(s) 470
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 4 cut(s) 243, 617, 1068, 1568
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 6 cut(s) 143, 156, 523, 935, 1149, 1275
Tth111I GACNNNGTC 1 cut(s) 1595
Vha464I CTTAAG 1 cut(s) 254
VneI GTGCAC 1 cut(s) 1140
VpaK11BI GGWCC 3 cut(s) 162, 336, 1429
VspI ATTAAT 1 cut(s) 435
XapI RAATTY 2 cut(s) 1499, 1616
XceI RCATGY 1 cut(s) 500
XcmI CCANNNNNNNNNTGG 1 cut(s) 111
XmiI GTMKAC 1 cut(s) 190
XspI CTAG 3 cut(s) 186, 297, 1412
ZrmI AGTACT 1 cut(s) 1159
Zsp2I ATGCAT 1 cut(s) 1537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.