Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004429

Physical Location & Seq

Genomic Coordinates Forward (+)

5649306 .. 5653528

Gene Structure 4223 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1034800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1179 bp

ATGTCGAAAACATTGGGGAATGTAATAGATCCATTAGACACAATCAAGGAATATGGCACAGATGCTTTAAGATTTACAATTGCTCTAGGGACTGCTGGCCAGGACCTTAATTTATCCACTGAGAGGTTGACATCCAACAAGGCCTTCACTAATAAACTGTGGAATGCTGGAAAGTTTGTATTGCAGAATTTACCCAGTCAAAGCGAAGCATCAACTTGGGAAAATATACTTTCTTATAAGTTTGATAAAGTGGATCTCCTAGACAAGCTACCCTTACCAGAATCTTGGGTGATTTCAAAACTTCATTTGCTTATTGACACAGTCACCGCAAGCTATGACAAATTTTTCTTTGGAGATGTTGGAAGAGAGACGTATGAATTCTTTTGGGGTGATTTTGCTGATTGGTATATAGAGGCCAGCAAAGCTCGTCTTTACCACTCTGGTGGTGATTCAGTTGCTTCTGTCACACAGGCAGTTCTATTGTATGTTTTTGAAAACATACTTAAATTGCTACACCCCTTTATGCCATTTGTGACCGAGGAACTCTGGCAGGCACTCCCCTTTCGGAAAGAAGCTCTTATTGTATCTCCATGGCCACTGACTTCACTTCCAAGGCAGAGCAACTCGATTAAAAAGTTTGAGAATTTACAGGCATTGACTAGAGCTATCAGAAATGCTCGAGCAGAATATTCTGTTGAGCCTGTAAAGCGTATATCAGCATCAATAGTTGCAAATGAAGAAGTCACTGAATACATAATGGCTGGATTTGCAAAACGTCCATTTTACAGATTCTCCTCCAGGCACATTTCTTCATGGATTATTTTACATTTGACAAAGGACCGGAAATGTTCATGGAACGCGGATCAATCAGTCCACCTTGTAGCTGGTGAAGGACTAGAGGCATATCTCCCTCTTGCTGACATGATTGATATTACTGCCGAAATCCAACGCCTATCTAAGAGACTTTCCAAGATGCAGGCGGAATATGATGGACTTAAAGCTCGGCTCAGCTCTCCTAAATTTGTAGAGAAAGCTCCTGAGGATATTGTCCGTGGAGTGCAAGAAAAGGCGGCAGAAAAACAGAAGAGAAGCTTACTTACTCTCACCAAAAAACCGTTTAGCATTACTCGAATCAAGTGTTCTGGTCACATAATAAGACCCTCTGCCTGTCATCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 Val_tRNA-synt_C tRNA-synt_1

Protein Analysis

393

Amino Acids

44.53

Weight (kDa)

8.97

Isoelectric Point (pI)

50.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	1 - 39	2.2e-08	tRNA synthetases class I (I, L, M and V)
Anticodon_1	PF08264	95 - 238	7.8e-40	Anticodon-binding domain of tRNA ligase
Val_tRNA-synt_C	PF10458	310 - 362	1e-12	Valyl tRNA synthetase tRNA binding arm

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004429)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16715
fragaria_vesca	FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130
malus_domestica	MD05G1034800.v1.1 MD09G1264300.v1.1 MD09G1264400.v1.1 MD17G1258800.v1.1 MD17G1259600.v1.1 MD17G1259800.v1.1 MD17G1260100.v1.1
prunus_persica	Prupe.3G161900_v2.0.a1 Prupe.3G162000_v2.0.a1
pyrus_communis	pycom09g17800 pycom17g26100
rosa_chinensis	RchiOBHm_Chr1g0328051
rosa_laevigata	RLG00000029942
rosa_multiflora	Rmu_sc0001829.1_g000010
rosa_roxburghii	Rroxscaffold_4G00321900
rosa_rugosa	Rorug01G0073400
rosa_samantha	Rh1AG090500 Rh1BG072400 Rh1CG087100 Rh1DG093800
rosa_wichuraiana	Rw1G007020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	237
AccII	CGCG	1 cut(s)	860
AciI	CCGC	4 cut(s)	327, 860, 980, 1070
AclWI	GGATC	3 cut(s)	23, 261, 870
AcoI	YGGCCR	2 cut(s)	97, 593
AcsI	RAATTY	5 cut(s)	187, 341, 377, 643, 1019
AfiI	CCNNNNNNNGG	1 cut(s)	123
AgsI	TTSAA	2 cut(s)	297, 494
AjnI	CCWGG	2 cut(s)	99, 797
AjuI	GAANNNNNNNTTGG	2 cut(s)	128, 160
AleI	CACNNNNGTG	1 cut(s)	441
Alw26I	GTCTC	2 cut(s)	362, 955
AlwI	GGATC	3 cut(s)	23, 261, 870
Ama87I	CYCGRG	1 cut(s)	678
AoxI	GGCC	4 cut(s)	97, 141, 414, 593
ApoI	RAATTY	5 cut(s)	187, 341, 377, 643, 1019
AspS9I	GGNCC	2 cut(s)	103, 838
AsuHPI	GGTGA	6 cut(s)	301, 316, 401, 458, 899, 1096
AvaI	CYCGRG	1 cut(s)	678
AvaII	GGWCC	2 cut(s)	103, 838
AxyI	CCTNAGG	1 cut(s)	1038
BalI	TGGCCA	2 cut(s)	99, 595
BarI	GAAGNNNNNNTAC	2 cut(s)	1081, 1113
BccI	CCATC	1 cut(s)	983
BciT130I	CCWGG	2 cut(s)	101, 799
BcoDI	GTCTC	2 cut(s)	362, 955
BfaI	CTAG	4 cut(s)	86, 260, 660, 896
BfmI	CTRYAG	1 cut(s)	1175
BisI	GCNGC	1 cut(s)	1071
BlpI	GCTNAGC	1 cut(s)	1007
BlsI	GCNGC	1 cut(s)	1072
Bme1390I	CCNGG	2 cut(s)	101, 799
Bme18I	GGWCC	2 cut(s)	103, 838
BmeT110I	CYCGRG	1 cut(s)	678
BmgT120I	GGNCC	2 cut(s)	103, 838
BmrFI	CCNGG	2 cut(s)	101, 799
BmrI	ACTGGG	1 cut(s)	189
BmsI	GCATC	4 cut(s)	52, 218, 728, 963
BmuI	ACTGGG	1 cut(s)	189
BpmI	CTGGAG	1 cut(s)	781
Bpu1102I	GCTNAGC	1 cut(s)	1007
BsaJI	CCNNGG	4 cut(s)	537, 590, 611, 1051
BsaWI	WCCGGW	1 cut(s)	840
BsaXI	ACNNNNNCTCC	2 cut(s)	776, 806
Bsc4I	CCNNNNNNNGG	1 cut(s)	123
Bse1I	ACTGG	1 cut(s)	195
Bse21I	CCTNAGG	1 cut(s)	1038
BseBI	CCWGG	2 cut(s)	101, 799
BseDI	CCNNGG	4 cut(s)	537, 590, 611, 1051
BseGI	GGATG	1 cut(s)	131
BseLI	CCNNNNNNNGG	1 cut(s)	123
BseMII	CTCAG	3 cut(s)	111, 1021, 1029
BseNI	ACTGG	1 cut(s)	195
BseRI	GAGGAG	1 cut(s)	784
Bsh1236I	CGCG	1 cut(s)	860
BshFI	GGCC	4 cut(s)	99, 143, 416, 595
BsiHKCI	CYCGRG	1 cut(s)	678
BsiSI	CCGG	1 cut(s)	841
BslFI	GGGAC	1 cut(s)	103
BslI	CCNNNNNNNGG	1 cut(s)	123
BsmAI	GTCTC	2 cut(s)	362, 955
BsmBI	CGTCTC	1 cut(s)	362
BsmFI	GGGAC	1 cut(s)	103
BsmI	GAATGC	1 cut(s)	169
BsnI	GGCC	4 cut(s)	99, 143, 416, 595
BsoBI	CYCGRG	1 cut(s)	678
Bsp143I	GATC	3 cut(s)	28, 253, 862
Bsp1720I	GCTNAGC	1 cut(s)	1007
Bsp19I	CCATGG	1 cut(s)	590
BspACI	CCGC	4 cut(s)	327, 860, 980, 1070
BspANI	GGCC	4 cut(s)	99, 143, 416, 595
BspCNI	CTCAG	3 cut(s)	112, 1020, 1030
BspFNI	CGCG	1 cut(s)	860
BspPI	GGATC	3 cut(s)	23, 261, 870
BsrI	ACTGG	1 cut(s)	195
BssECI	CCNNGG	4 cut(s)	537, 590, 611, 1051
BssMI	GATC	3 cut(s)	28, 253, 862
BssT1I	CCWWGG	2 cut(s)	590, 611
Bst2UI	CCWGG	2 cut(s)	101, 799
Bst4CI	ACNGT	3 cut(s)	159, 322, 1116
Bst6I	CTCTTC	2 cut(s)	358, 1079
BstC8I	GCNNGC	5 cut(s)	97, 331, 418, 552, 978
BstDEI	CTNAG	4 cut(s)	120, 957, 1007, 1038
BstDSI	CCRYGG	2 cut(s)	590, 1051
BstF5I	GGATG	1 cut(s)	131
BstFNI	CGCG	1 cut(s)	860
BstKTI	GATC	3 cut(s)	31, 256, 865
BstMAI	GTCTC	2 cut(s)	362, 955
BstMBI	GATC	3 cut(s)	28, 253, 862
BstMWI	GCNNNNNNNGC	3 cut(s)	422, 706, 767
BstNI	CCWGG	2 cut(s)	101, 799
BstSCI	CCNGG	2 cut(s)	99, 797
BstSFI	CTRYAG	1 cut(s)	1175
BstUI	CGCG	1 cut(s)	860
BstX2I	RGATCY	2 cut(s)	28, 253
BstXI	CCANNNNNNTGG	2 cut(s)	285, 443
BstYI	RGATCY	2 cut(s)	28, 253
Bsu36I	CCTNAGG	1 cut(s)	1038
BsuRI	GGCC	4 cut(s)	99, 143, 416, 595
BtgI	CCRYGG	2 cut(s)	590, 1051
BtsCI	GGATG	1 cut(s)	131
BtsIMutI	CAGTG	3 cut(s)	117, 596, 744
Cac8I	GCNNGC	5 cut(s)	97, 331, 418, 552, 978
Cfr13I	GGNCC	2 cut(s)	103, 838
CviAII	CATG	4 cut(s)	591, 813, 852, 922
DdeI	CTNAG	4 cut(s)	120, 957, 1007, 1038
DpnI	GATC	3 cut(s)	30, 255, 864
DpnII	GATC	3 cut(s)	28, 253, 862
EaeI	YGGCCR	2 cut(s)	97, 593
Eam1104I	CTCTTC	2 cut(s)	358, 1079
EarI	CTCTTC	2 cut(s)	358, 1079
EciI	GGCGGA	1 cut(s)	995
Eco130I	CCWWGG	2 cut(s)	590, 611
Eco147I	AGGCCT	1 cut(s)	143
Eco47I	GGWCC	2 cut(s)	103, 838
Eco81I	CCTNAGG	1 cut(s)	1038
Eco88I	CYCGRG	1 cut(s)	678
EcoO109I	RGGNCCY	1 cut(s)	103
EcoRI	GAATTC	1 cut(s)	377
EcoRII	CCWGG	2 cut(s)	99, 797
EcoT14I	CCWWGG	2 cut(s)	590, 611
ErhI	CCWWGG	2 cut(s)	590, 611
Esp3I	CGTCTC	1 cut(s)	362
FaeI	CATG	4 cut(s)	594, 816, 855, 925
FalI	AAGNNNNNCTT	8 cut(s)	257, 289, 414, 446, 561, 593, 1076, 1108
FaqI	GGGAC	1 cut(s)	103
FatI	CATG	4 cut(s)	590, 812, 851, 921
Fnu4HI	GCNGC	1 cut(s)	1071
FokI	GGATG	1 cut(s)	118
Fsp4HI	GCNGC	1 cut(s)	1071
FspBI	CTAG	4 cut(s)	86, 260, 660, 896
GluI	GCNGC	1 cut(s)	1071
GsuI	CTGGAG	1 cut(s)	781
HaeIII	GGCC	4 cut(s)	99, 143, 416, 595
HapII	CCGG	1 cut(s)	841
Hin1II	CATG	4 cut(s)	594, 816, 855, 925
HincII	GTYRAC	1 cut(s)	129
HindII	GTYRAC	1 cut(s)	129
HindIII	AAGCTT	1 cut(s)	1090
HinfI	GANTC	4 cut(s)	281, 449, 789, 1131
HpaII	CCGG	1 cut(s)	841
HphI	GGTGA	6 cut(s)	301, 316, 401, 458, 899, 1096
Hpy166II	GTNNAC	2 cut(s)	129, 874
Hpy188I	TCNGA	2 cut(s)	567, 671
Hpy188III	TCNNGA	1 cut(s)	1037
Hpy8I	GTNNAC	2 cut(s)	129, 874
HpyAV	CCTTC	2 cut(s)	154, 884
HpyCH4III	ACNGT	3 cut(s)	159, 322, 1116
HpyCH4IV	ACGT	2 cut(s)	371, 775
HpyCH4V	TGCA	5 cut(s)	184, 731, 770, 976, 1060
HpyF10VI	GCNNNNNNNGC	3 cut(s)	422, 706, 767
HpyF3I	CTNAG	4 cut(s)	120, 957, 1007, 1038
HpySE526I	ACGT	2 cut(s)	371, 775
Hsp92II	CATG	4 cut(s)	594, 816, 855, 925
Kzo9I	GATC	3 cut(s)	28, 253, 862
LmnI	GCTCC	1 cut(s)	1039
LweI	GCATC	4 cut(s)	52, 218, 728, 963
MaeI	CTAG	4 cut(s)	86, 260, 660, 896
MaeII	ACGT	2 cut(s)	371, 775
MaeIII	GTNAC	5 cut(s)	322, 463, 532, 742, 1145
MalI	GATC	3 cut(s)	30, 255, 864
MboI	GATC	3 cut(s)	28, 253, 862
MboII	GAAGA	4 cut(s)	375, 749, 801, 1096
MfeI	CAATTG	1 cut(s)	78
MflI	RGATCY	2 cut(s)	28, 253
MlsI	TGGCCA	2 cut(s)	99, 595
MluCI	AATT	8 cut(s)	78, 109, 187, 341, 377, 506, 643, 1019
MluNI	TGGCCA	2 cut(s)	99, 595
MmeI	TCCRAC	3 cut(s)	159, 340, 970
MnlI	CCTC	8 cut(s)	117, 406, 532, 805, 892, 921, 1033, 1171
Mox20I	TGGCCA	2 cut(s)	99, 595
MscI	TGGCCA	2 cut(s)	99, 595
MseI	TTAA	5 cut(s)	68, 108, 504, 630, 996
MslI	CAYNNNNRTG	1 cut(s)	441
Msp20I	TGGCCA	2 cut(s)	99, 595
MspI	CCGG	1 cut(s)	841
MspR9I	CCNGG	2 cut(s)	101, 799
MunI	CAATTG	1 cut(s)	78
Mva1269I	GAATGC	1 cut(s)	169
MvaI	CCWGG	2 cut(s)	101, 799
MvnI	CGCG	1 cut(s)	860
MwoI	GCNNNNNNNGC	3 cut(s)	422, 706, 767
NcoI	CCATGG	1 cut(s)	590
NdeII	GATC	3 cut(s)	28, 253, 862
NlaIII	CATG	4 cut(s)	594, 816, 855, 925
NmeAIII	GCCGAG	1 cut(s)	982
NmuCI	GTSAC	5 cut(s)	322, 463, 532, 742, 1145
OliI	CACNNNNGTG	1 cut(s)	441
PaeR7I	CTCGAG	1 cut(s)	678
PceI	AGGCCT	1 cut(s)	143
PctI	GAATGC	1 cut(s)	169
PfeI	GAWTC	4 cut(s)	281, 449, 789, 1131
PflFI	GACNNNGTC	1 cut(s)	320
PkrI	GCNGC	1 cut(s)	1072
PpuMI	RGGWCCY	1 cut(s)	103
PsiI	TTATAA	1 cut(s)	237
Psp5II	RGGWCCY	1 cut(s)	103
Psp6I	CCWGG	2 cut(s)	99, 797
PspGI	CCWGG	2 cut(s)	99, 797
PspPI	GGNCC	2 cut(s)	103, 838
PspPPI	RGGWCCY	1 cut(s)	103
PspXI	VCTCGAGB	1 cut(s)	678
PsuI	RGATCY	2 cut(s)	28, 253
PsyI	GACNNNGTC	1 cut(s)	320
RseI	CAYNNNNRTG	1 cut(s)	441
SaqAI	TTAA	5 cut(s)	68, 108, 504, 630, 996
SatI	GCNGC	1 cut(s)	1071
Sau3AI	GATC	3 cut(s)	28, 253, 862
Sau96I	GGNCC	2 cut(s)	103, 838
ScrFI	CCNGG	2 cut(s)	101, 799
SfaNI	GCATC	4 cut(s)	52, 218, 728, 963
SfcI	CTRYAG	1 cut(s)	1175
Sfr274I	CTCGAG	1 cut(s)	678
SinI	GGWCC	2 cut(s)	103, 838
SlaI	CTCGAG	1 cut(s)	678
SmiMI	CAYNNNNRTG	1 cut(s)	441
SmlI	CTYRAG	1 cut(s)	678
SmoI	CTYRAG	1 cut(s)	678
Sse9I	AATT	8 cut(s)	78, 109, 187, 341, 377, 506, 643, 1019
SseBI	AGGCCT	1 cut(s)	143
SsiI	CCGC	4 cut(s)	327, 860, 980, 1070
SspI	AATATT	1 cut(s)	689
SspMI	CTAG	4 cut(s)	86, 260, 660, 896
StuI	AGGCCT	1 cut(s)	143
StyD4I	CCNGG	2 cut(s)	99, 797
StyI	CCWWGG	2 cut(s)	590, 611
TaaI	ACNGT	3 cut(s)	159, 322, 1116
TaiI	ACGT	2 cut(s)	374, 778
TaqI	TCGA	4 cut(s)	5, 626, 679, 1129
TaqII	GACCGA	1 cut(s)	551
TasI	AATT	8 cut(s)	78, 109, 187, 341, 377, 506, 643, 1019
TauI	GCSGC	1 cut(s)	1073
TfiI	GAWTC	4 cut(s)	281, 449, 789, 1131
Tru1I	TTAA	5 cut(s)	68, 108, 504, 630, 996
Tru9I	TTAA	5 cut(s)	68, 108, 504, 630, 996
TscAI	CASTG	3 cut(s)	124, 603, 751
TseFI	GTSAC	5 cut(s)	322, 463, 532, 742, 1145
Tsp45I	GTSAC	5 cut(s)	322, 463, 532, 742, 1145
TspDTI	ATGAA	5 cut(s)	293, 390, 750, 801, 840
TspGWI	ACGGA	1 cut(s)	1040
TspRI	CASTG	3 cut(s)	124, 603, 751
Tth111I	GACNNNGTC	1 cut(s)	320
VpaK11BI	GGWCC	2 cut(s)	103, 838
XapI	RAATTY	5 cut(s)	187, 341, 377, 643, 1019
XcmI	CCANNNNNNNNNTGG	1 cut(s)	881
XhoI	CTCGAG	1 cut(s)	678
XspI	CTAG	4 cut(s)	86, 260, 660, 896

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.