AT5G16720

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007066
Physical Location & Seq
Forward (+)
5494228 .. 5496846
2619 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16720.1

Sequence Viewer

Length: 2028 bp
ATGGCGGCCAACAATTTTGCGACGAAGCTAAGCAGAAACACCAATAGAATTACCGTAATCTTGGTCTACGCATTCCTTGAATGGCTTCTCATGTTCTTTATCTTCCTCAATTCCTTTTTCACTTACTTTATCGTCAAATTCGCCTCTTTCTTCGGTCTTAAGCAAGTATGTCTTCTCTGTCCGAAGCTAGATCGAATCTTCGAGCGAAAGCCAGAAAACAGATTCACTTACAAAGAGCTTTTATGTCAAAATCACATCGCCGAGCTCGCTAGTCTGAGTTTTTGCAGAACTCACGGGAAATTATCCGAATCGGCGAACTTATGCTCAGATTGCTCTAATCGCGAAGAAGAACAGAGCAACATTGGTTTAGGTTTTTGCACATGTTGTCAGAAGAGCTTAGCGGATAAACCATACCCTAATTACTTGCTTCTCAAGTCATCAATTTGGGGGAAAACTTTGGGTGACAGAGAAGATGGAGGATTGATCTTAGAGATGATCGATGATGATAAATTTGGAGATGGGTTTGAGATAGATAGAGAATCTTATCCCTTAGGGTTCTTCAGAGATAAAGCAGAAGAAGGAAAGAAGCAAGACCAGCAGCAAAATGGTGAAGTGATTTCAGATGTTGAGAGTTATGGATTAAGCTTGAGAGAAGTGTCTGAAGAGGATGGGTTAAGATCTATCATCAGCAATAATTCTCCTGGGAACGAAGCTAAATCAAGAGTTTCAGAGGATGAACAAAGGAATGATGACACAAGCAATGTTGCCACATATGGGGAAGATCAAATTTCAGGGCGTGTAGAAGAAAAAGAAGAAGAAACAGGAGTAGCAGATTTGTTGTATGACCAGTTTGAGAGCAAGAATTTCACTGGTAGCCAAATCGAAGAAGAAGAAGAAGATAGAGAAGAAACGACTAAGGAGCTTGATCCGGAAACTCCAACTTCAGTCAGTACTTTGTTTAACAAGAAACTTCATTTTCTTGCTAGAAATGAGTATGCAGCTGCTGAAGATGCAGGAGATGGGAATGTATTAGTGAGTGAGATGGACGGTGGCGATCCTTTAAGGACTATTGAAAGGTTGAGAGAGACAGTGAGAGCAGAACAAGAAGCACTGAGAGATTTATATGCAGAGCTGGAAGAAGAAAGAAGCGCGTCTGCAATATCGGCTAACCAGACAATGGCAATGATCACGAGGCTACAAGAAGAGAAAGCGAAAGTTCAGATGGAAGCGTTGCAGTATCAGAGAATGATGGAGGAACAAGCCGAGTATGATCAAGAAGCCTTGCAGCTATTGAATCATTTGATGGTGAAACGAGAGAAAGAGAAGGAACAACTCCAGAGAGAACTTGAGGTTTATCGGGCAAAGGTTTTGGAGTATGAATCGAAAGCTAAGAACAAGATCATCGTTGTGGAAAACGATTGTGAGGCTGATGATGATGATAAAGAGGAGGAGAATAGAGAAGAGGATAATTCTTCTGAGATGGATGTGGATTTGGAGAAGATAACATTAGATTGCGTACAACATATGAGCATGCTTGGTGAATCTTTATCAGAGTTTGAGGAAGAGAGGTTAGTGATTTTGGATCAGCTTAAGGTGTTAGAGGATAGGCTAGTAACAATGCAGGACAAGGAATCTGCTGAGGATCCGGGAGAATTCAGCAATAGTTATGAAGAAGCGAGTAATGGACATGGAGGACTAACAATGGCATCAATGGCAAAGAGTCTGCTACCTCTTCTAGACGCAGCAGAGAACGAATCAGAAGACGGGTCTCAAGGACTACCAGAATCAGATGAAAAGAACTTTGGTTCGGACAGTGAGAAACTGGAGATAATCAAACAAGTTGATAGTGTCTATGAGAGGTTACAGGAGCTGGAAACAGATGGAGAGTTTCTGAAAAACTGTATGAGTTCAGCGAAGAAAGGTGACAAAGGAACAGATATTCTTAAAGATATATTGCAACATCTCCGCGATCTCAGAACCATTGAACTCACTAACACAATTGAAAACCAGACGACACAGGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008092 GO:0017022
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

675

Amino Acids

77.02

Weight (kDa)

4.43

Isoelectric Point (pI)

47.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 359 - 449 5.8e-32 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1177
AccI GTMKAC 1 cut(s) 66
AccII CGCG 3 cut(s) 342, 1151, 1968
AccIII TCCGGA 1 cut(s) 928
AciI CCGC 3 cut(s) 5, 401, 1966
AclWI GGATC 5 cut(s) 920, 1049, 1590, 1637, 1650
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 5 cut(s) 137, 509, 786, 862, 1652
AcuI CTGAAG 4 cut(s) 544, 681, 927, 1026
AfaI GTAC 2 cut(s) 952, 1518
AfiI CCNNNNNNNGG 2 cut(s) 774, 1177
AflII CTTAAG 2 cut(s) 158, 1589
AflIII ACRYGT 1 cut(s) 380
AgsI TTSAA 5 cut(s) 80, 1073, 1294, 1985, 2003
AjnI CCWGG 1 cut(s) 700
AjuI GAANNNNNNNTTGG 2 cut(s) 1785, 1817
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 2 cut(s) 1079, 1773
AlwI GGATC 5 cut(s) 920, 1049, 1590, 1637, 1650
AlwNI CAGNNNCTG 2 cut(s) 1004, 1870
Aor13HI TCCGGA 1 cut(s) 928
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 5 cut(s) 598, 998, 1001, 1285, 1742
ApoI RAATTY 5 cut(s) 137, 509, 786, 862, 1652
Asp700I GAANNNNTTC 1 cut(s) 84
AspLEI GCGC 1 cut(s) 1151
AsuC2I CCSGG 1 cut(s) 1647
AsuHPI GGTGA 5 cut(s) 473, 620, 1318, 1550, 1934
AxyI CCTNAGG 1 cut(s) 550
BamHI GGATCC 1 cut(s) 1642
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 1 cut(s) 1189
BbsI GAAGAC 2 cut(s) 164, 1767
Bbv12I GWGCWC 1 cut(s) 267
BbvCI CCTCAGC 1 cut(s) 1638
BbvI GCAGC 5 cut(s) 610, 988, 1010, 1297, 1754
BciT130I CCWGG 1 cut(s) 702
BclI TGATCA 2 cut(s) 1185, 1270
BcnI CCSGG 1 cut(s) 1647
BcoDI GTCTC 2 cut(s) 1079, 1773
BfaI CTAG 5 cut(s) 188, 270, 984, 1610, 1736
BfrI CTTAAG 2 cut(s) 158, 1589
BglII AGATCT 1 cut(s) 677
BisI GCNGC 6 cut(s) 6, 599, 999, 1002, 1286, 1743
BlpI GCTNAGC 2 cut(s) 29, 397
BlsI GCNGC 6 cut(s) 7, 600, 1000, 1003, 1287, 1744
BmcAI AGTACT 1 cut(s) 952
Bme1390I CCNGG 2 cut(s) 702, 1647
BmiI GGNNCC 1 cut(s) 1644
BmrFI CCNGG 2 cut(s) 702, 1647
BmsI GCATC 2 cut(s) 1000, 1715
BpiI GAAGAC 2 cut(s) 164, 1767
BpmI CTGGAG 2 cut(s) 1319, 1844
Bpu10I CCTNAGC 1 cut(s) 1638
Bpu1102I GCTNAGC 2 cut(s) 29, 397
BpuEI CTTGAG 4 cut(s) 416, 667, 1367, 1755
BpuMI CCSGG 1 cut(s) 1647
Bsa29I ATCGAT 1 cut(s) 498
BsaI GGTCTC 1 cut(s) 1773
BsaJI CCNNGG 1 cut(s) 701
BsaWI WCCGGW 1 cut(s) 928
Bsc4I CCNNNNNNNGG 2 cut(s) 774, 1177
Bse1I ACTGG 3 cut(s) 847, 874, 1827
Bse21I CCTNAGG 1 cut(s) 550
Bse3DI GCAATG 2 cut(s) 766, 1188
BseAI TCCGGA 1 cut(s) 928
BseBI CCWGG 1 cut(s) 702
BseCI ATCGAT 1 cut(s) 498
BseDI CCNNGG 1 cut(s) 701
BseGI GGATG 3 cut(s) 673, 739, 1489
BseLI CCNNNNNNNGG 2 cut(s) 774, 1177
BseMI GCAATG 2 cut(s) 766, 1188
BseMII CTCAG 6 cut(s) 266, 339, 1103, 1467, 1629, 1987
BseNI ACTGG 3 cut(s) 847, 874, 1827
BseRI GAGGAG 2 cut(s) 1460, 1463
BseXI GCAGC 5 cut(s) 610, 988, 1010, 1297, 1754
Bsh1236I CGCG 3 cut(s) 342, 1151, 1968
BshFI GGCC 1 cut(s) 8
BshVI ATCGAT 1 cut(s) 498
BsiHKAI GWGCWC 1 cut(s) 267
BsiSI CCGG 2 cut(s) 929, 1646
BslI CCNNNNNNNGG 2 cut(s) 774, 1177
BsmAI GTCTC 2 cut(s) 1079, 1773
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 1 cut(s) 8
Bso31I GGTCTC 1 cut(s) 1773
Bsp1286I GDGCHC 1 cut(s) 267
Bsp13I TCCGGA 1 cut(s) 928
Bsp1720I GCTNAGC 2 cut(s) 29, 397
Bsp68I TCGCGA 1 cut(s) 342
BspACI CCGC 3 cut(s) 5, 401, 1966
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 6 cut(s) 267, 338, 1104, 1468, 1630, 1986
BspDI ATCGAT 1 cut(s) 498
BspEI TCCGGA 1 cut(s) 928
BspFNI CGCG 3 cut(s) 342, 1151, 1968
BspLI GGNNCC 1 cut(s) 1644
BspPI GGATC 5 cut(s) 920, 1049, 1590, 1637, 1650
BspQI GCTCTTC 1 cut(s) 386
BspTI CTTAAG 2 cut(s) 158, 1589
BspTNI GGTCTC 1 cut(s) 1773
BsrDI GCAATG 2 cut(s) 766, 1188
BsrI ACTGG 3 cut(s) 847, 874, 1827
BssECI CCNNGG 1 cut(s) 701
BssSI CACGAG 1 cut(s) 1189
Bst2BI CACGAG 1 cut(s) 1189
Bst2UI CCWGG 1 cut(s) 702
Bst4CI ACNGT 5 cut(s) 55, 1049, 1090, 1814, 1901
Bst6I CTCTTC 7 cut(s) 386, 657, 1197, 1455, 1557, 1737, 2016
BstAFI CTTAAG 2 cut(s) 158, 1589
BstC8I GCNNGC 2 cut(s) 267, 1532
BstF5I GGATG 3 cut(s) 673, 739, 1489
BstFNI CGCG 3 cut(s) 342, 1151, 1968
BstHHI GCGC 1 cut(s) 1151
BstMAI GTCTC 2 cut(s) 1079, 1773
BstMWI GCNNNNNNNGC 7 cut(s) 266, 330, 339, 595, 1010, 1163, 1712
BstNI CCWGG 1 cut(s) 702
BstNSI RCATGY 2 cut(s) 384, 1534
BstSCI CCNGG 2 cut(s) 700, 1645
BstUI CGCG 3 cut(s) 342, 1151, 1968
BstV1I GCAGC 5 cut(s) 610, 988, 1010, 1297, 1754
BstV2I GAAGAC 2 cut(s) 164, 1767
BstX2I RGATCY 2 cut(s) 677, 1642
BstYI RGATCY 2 cut(s) 677, 1642
Bsu15I ATCGAT 1 cut(s) 498
Bsu36I CCTNAGG 1 cut(s) 550
BsuRI GGCC 1 cut(s) 8
BsuTUI ATCGAT 1 cut(s) 498
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 3 cut(s) 673, 739, 1489
BtsIMutI CAGTG 4 cut(s) 867, 1095, 1109, 1819
BtuMI TCGCGA 1 cut(s) 342
Cac8I GCNNGC 2 cut(s) 267, 1532
CaiI CAGNNNCTG 2 cut(s) 1004, 1870
CfoI GCGC 1 cut(s) 1151
ClaI ATCGAT 1 cut(s) 498
CseI GACGC 2 cut(s) 1140, 1748
Csp6I GTAC 2 cut(s) 951, 1517
CviAII CATG 4 cut(s) 91, 381, 1531, 1688
CviQI GTAC 2 cut(s) 951, 1517
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 7 cut(s) 386, 657, 1197, 1455, 1557, 1737, 2016
EarI CTCTTC 7 cut(s) 386, 657, 1197, 1455, 1557, 1737, 2016
Ecl136II GAGCTC 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 1 cut(s) 1773
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 4 cut(s) 544, 681, 927, 1026
Eco81I CCTNAGG 1 cut(s) 550
EcoICRI GAGCTC 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 1652
EcoRII CCWGG 1 cut(s) 700
EcoT38I GRGCYC 1 cut(s) 267
FaeI CATG 4 cut(s) 94, 384, 1534, 1691
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FatI CATG 4 cut(s) 90, 380, 1530, 1687
FauNDI CATATG 2 cut(s) 772, 1524
FbaI TGATCA 2 cut(s) 1185, 1270
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 6 cut(s) 6, 599, 999, 1002, 1286, 1743
FokI GGATG 3 cut(s) 680, 746, 1496
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 6 cut(s) 6, 599, 999, 1002, 1286, 1743
FspBI CTAG 5 cut(s) 188, 270, 984, 1610, 1736
GlaI GCGC 1 cut(s) 1150
GluI GCNGC 6 cut(s) 6, 599, 999, 1002, 1286, 1743
GsuI CTGGAG 2 cut(s) 1319, 1844
HaeIII GGCC 1 cut(s) 8
HapII CCGG 2 cut(s) 929, 1646
HgaI GACGC 2 cut(s) 1140, 1748
HhaI GCGC 1 cut(s) 1151
Hin1II CATG 4 cut(s) 94, 384, 1534, 1691
Hin6I GCGC 1 cut(s) 1149
HinP1I GCGC 1 cut(s) 1149
HindIII AAGCTT 1 cut(s) 643
HpaII CCGG 2 cut(s) 929, 1646
HphI GGTGA 5 cut(s) 473, 620, 1318, 1550, 1934
Hpy166II GTNNAC 1 cut(s) 67
Hpy188III TCNNGA 7 cut(s) 341, 720, 929, 1189, 1274, 1336, 1736
Hpy8I GTNNAC 1 cut(s) 67
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 2 cut(s) 572, 1318
HpyCH4III ACNGT 5 cut(s) 55, 1049, 1090, 1814, 1901
HpyF10VI GCNNNNNNNGC 7 cut(s) 266, 330, 339, 595, 1010, 1163, 1712
Hsp92II CATG 4 cut(s) 94, 384, 1534, 1691
HspAI GCGC 1 cut(s) 1149
Kpn2I TCCGGA 1 cut(s) 928
Ksp22I TGATCA 2 cut(s) 1185, 1270
LguI GCTCTTC 1 cut(s) 386
LmnI GCTCC 2 cut(s) 919, 1867
Lsp1109I GCAGC 5 cut(s) 610, 988, 1010, 1297, 1754
LweI GCATC 2 cut(s) 1000, 1715
MaeI CTAG 5 cut(s) 188, 270, 984, 1610, 1736
MaeIII GTNAC 4 cut(s) 461, 1612, 1860, 1922
MfeI CAATTG 1 cut(s) 1998
MflI RGATCY 2 cut(s) 677, 1642
MhlI GDGCHC 1 cut(s) 267
MlyI GAGTC 1 cut(s) 1729
MmeI TCCRAC 1 cut(s) 962
MroI TCCGGA 1 cut(s) 928
MroXI GAANNNNTTC 1 cut(s) 84
MseI TTAA 7 cut(s) 159, 641, 674, 960, 1061, 1590, 1944
MslI CAYNNNNRTG 1 cut(s) 1406
MspA1I CMGCKG 1 cut(s) 1001
MspCI CTTAAG 2 cut(s) 158, 1589
MspI CCGG 2 cut(s) 929, 1646
MspR9I CCNGG 2 cut(s) 702, 1647
MunI CAATTG 1 cut(s) 1998
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 1 cut(s) 702
MvnI CGCG 3 cut(s) 342, 1151, 1968
MwoI GCNNNNNNNGC 7 cut(s) 266, 330, 339, 595, 1010, 1163, 1712
NciI CCSGG 1 cut(s) 1647
NdeI CATATG 2 cut(s) 772, 1524
NlaIII CATG 4 cut(s) 94, 384, 1534, 1691
NlaIV GGNNCC 1 cut(s) 1644
NmeAIII GCCGAG 2 cut(s) 286, 1288
NmuCI GTSAC 2 cut(s) 461, 1922
NruI TCGCGA 1 cut(s) 342
NspI RCATGY 2 cut(s) 384, 1534
PaeI GCATGC 1 cut(s) 1534
PciI ACATGT 1 cut(s) 380
PciSI GCTCTTC 1 cut(s) 386
PctI GAATGC 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 84
PflMI CCANNNNNTGG 1 cut(s) 1177
PfoI TCCNGGA 1 cut(s) 1645
PkrI GCNGC 6 cut(s) 7, 600, 1000, 1003, 1287, 1744
PleI GAGTC 1 cut(s) 1728
PpsI GAGTC 1 cut(s) 1728
PscI ACATGT 1 cut(s) 380
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 700
PspGI CCWGG 1 cut(s) 700
PspN4I GGNNCC 1 cut(s) 1644
PstNI CAGNNNCTG 2 cut(s) 1004, 1870
PsuI RGATCY 2 cut(s) 677, 1642
PvuII CAGCTG 1 cut(s) 1001
RruI TCGCGA 1 cut(s) 342
RsaI GTAC 2 cut(s) 952, 1518
RsaNI GTAC 2 cut(s) 951, 1517
RseI CAYNNNNRTG 1 cut(s) 1406
SacI GAGCTC 1 cut(s) 267
SapI GCTCTTC 1 cut(s) 386
SaqAI TTAA 7 cut(s) 159, 641, 674, 960, 1061, 1590, 1944
SatI GCNGC 6 cut(s) 6, 599, 999, 1002, 1286, 1743
ScaI AGTACT 1 cut(s) 952
SchI GAGTC 1 cut(s) 1729
ScrFI CCNGG 2 cut(s) 702, 1647
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 2 cut(s) 1000, 1715
SmiMI CAYNNNNRTG 1 cut(s) 1406
SmlI CTYRAG 6 cut(s) 158, 431, 646, 1346, 1589, 1770
SmoI CTYRAG 6 cut(s) 158, 431, 646, 1346, 1589, 1770
SphI GCATGC 1 cut(s) 1534
SsiI CCGC 3 cut(s) 5, 401, 1966
SspMI CTAG 5 cut(s) 188, 270, 984, 1610, 1736
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 2 cut(s) 700, 1645
TaaI ACNGT 5 cut(s) 55, 1049, 1090, 1814, 1901
TaqI TCGA 5 cut(s) 193, 201, 498, 882, 1382
TaqII GACCGA 1 cut(s) 143
TatI WGTACW 1 cut(s) 950
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 7 cut(s) 159, 641, 674, 960, 1061, 1590, 1944
Tru9I TTAA 7 cut(s) 159, 641, 674, 960, 1061, 1590, 1944
TscAI CASTG 4 cut(s) 874, 1095, 1116, 1819
TseFI GTSAC 2 cut(s) 461, 1922
TseI GCWGC 5 cut(s) 598, 998, 1001, 1285, 1742
Tsp45I GTSAC 2 cut(s) 461, 1922
TspDTI ATGAA 5 cut(s) 750, 962, 1392, 1683, 1806
TspRI CASTG 4 cut(s) 874, 1095, 1116, 1819
Van91I CCANNNNNTGG 1 cut(s) 1177
Vha464I CTTAAG 2 cut(s) 158, 1589
XapI RAATTY 5 cut(s) 137, 509, 786, 862, 1652
XbaI TCTAGA 1 cut(s) 1735
XceI RCATGY 2 cut(s) 384, 1534
XcmI CCANNNNNNNNNTGG 1 cut(s) 602
XmiI GTMKAC 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 84
XspI CTAG 5 cut(s) 188, 270, 984, 1610, 1736
ZrmI AGTACT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.