Rorug03G0259400

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007066
Physical Location & Seq
Reverse (-)
24946241 .. 24950598
4358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0259400.1

Sequence Viewer

Length: 903 bp
ATGGGATTGCCTGATTCTTCTGGAGGTTTGCTGTCTGAATTGGACGTCGATGCTTTTAGACGCCTATTTCCTCTTCGGTTTTATGAGAGCCATCTTGCCAAGTCCACACGCCCTGATGGCAGGTCACTTGCCAAAGCTAGAGACACTTCCTCAGCCCTTGGGGCTGTTGCATCTGCCGATGGATCTGCGCTAGTAAAAATTGGTTCGACGACAATGCTGGCTGCTATAAAAATGGAAGTCATGACCCCTTCTCAAGAGTCACCAGATGAGGGATGCATAGCAATTGAATTCCACATGCCTCCAATTTGCTCTCCAATTGTAAGGCCTGGCAGGCCAGCTGAGGCAGCACCTGTTGTGTCAAAACAGCTATCTGACACTATTTTAAGTTCCGGCATGATTAATTTGAAGGAATTATCCTTGATTAGTGGAAAAGCTGCTTGGATGGTCTACCTGGACATATACTGTTTGGATGCTGATGGTGCTCTATTTGATGCTGCTTTGCTGTCAGCAGTCGCTGCCTTTTCTCACTTGCAGATTCCTGTAGTTTCTATGAATGATGAAGGGAAAGTAGTAGTCATGTCTGAGGAAAATGAGGGAGGAAAGGAGCCAGTCAATAAAGAGAAGAGGAAACTCACGTTATGCAGTATTCCATTTTCACTAACATGCATACTTTATAAGAATTACATCTTGGCTGACCCTACAGCAGAGGAAGAGTCGATCATGGAAACTCTTGTAACCGTGGTTTTGGATTCATCTGGTCAACTTGTGTCTCTTTACAAGCCAGGTGGACCAGTTCTTGCCTATACATCAGCTATCCAGGATTGTGTGGCACTCACTAGGCAAAGAGTGAGGGAGCTTCAGACGATTCTAGATGAAGCCACCGCTGGTATGGAGGTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008092 GO:0017022
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

300

Amino Acids

32.09

Weight (kDa)

4.76

Isoelectric Point (pI)

47.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_PH PF01138 46 - 180 1.8e-25 3' exoribonuclease family, domain 1
RNase_PH_C PF03725 216 - 265 2.5e-09 3' exoribonuclease family, domain 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 675
AatII GACGTC 1 cut(s) 48
Acc36I ACCTGC 1 cut(s) 111
AccI GTMKAC 1 cut(s) 447
AciI CCGC 1 cut(s) 882
AclWI GGATC 1 cut(s) 190
AcsI RAATTY 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 842
AcyI GRCGYC 2 cut(s) 45, 61
AfiI CCNNNNNNNGG 1 cut(s) 269
AgsI TTSAA 2 cut(s) 287, 406
AjnI CCWGG 4 cut(s) 325, 450, 781, 816
AjuI GAANNNNNNNTTGG 4 cut(s) 421, 453, 671, 703
AluBI AGCT 6 cut(s) 137, 338, 367, 434, 812, 856
AluI AGCT 6 cut(s) 137, 338, 367, 434, 812, 856
Alw21I GWGCWC 1 cut(s) 484
Alw26I GTCTC 2 cut(s) 135, 774
AlwI GGATC 1 cut(s) 190
AlwNI CAGNNNCTG 2 cut(s) 350, 515
AoxI GGCC 2 cut(s) 323, 332
ApeKI GCWGC 5 cut(s) 221, 344, 434, 494, 515
ApoI RAATTY 1 cut(s) 287
AseI ATTAAT 1 cut(s) 399
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 1 cut(s) 788
AsuHPI GGTGA 1 cut(s) 252
AvaII GGWCC 1 cut(s) 788
Bbv12I GWGCWC 1 cut(s) 484
BbvCI CCTCAGC 2 cut(s) 151, 339
BbvI GCAGC 5 cut(s) 208, 356, 421, 481, 502
BccI CCATC 5 cut(s) 99, 110, 173, 436, 470
BcgI CGANNNNNNTGC 4 cut(s) 167, 196, 201, 230
BciT130I CCWGG 4 cut(s) 327, 452, 783, 818
BcoDI GTCTC 2 cut(s) 135, 774
BfaI CTAG 4 cut(s) 138, 191, 837, 869
BfmI CTRYAG 2 cut(s) 540, 699
BfuAI ACCTGC 1 cut(s) 111
BglI GCCNNNNNGGC 3 cut(s) 117, 161, 331
BisI GCNGC 5 cut(s) 222, 345, 435, 495, 516
BlsI GCNGC 5 cut(s) 223, 346, 436, 496, 517
Bme1390I CCNGG 4 cut(s) 327, 452, 783, 818
Bme18I GGWCC 1 cut(s) 788
BmgT120I GGNCC 1 cut(s) 788
BmiI GGNNCC 1 cut(s) 606
BmrFI CCNGG 4 cut(s) 327, 452, 783, 818
BmsI GCATC 5 cut(s) 40, 179, 263, 460, 481
BpmI CTGGAG 1 cut(s) 42
Bpu10I CCTNAGC 2 cut(s) 151, 339
BpuEI CTTGAG 1 cut(s) 237
BsaHI GRCGYC 2 cut(s) 45, 61
BsaJI CCNNGG 2 cut(s) 157, 738
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse1I ACTGG 2 cut(s) 608, 791
BseBI CCWGG 4 cut(s) 327, 452, 783, 818
BseDI CCNNGG 2 cut(s) 157, 738
BseGI GGATG 3 cut(s) 278, 447, 475
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMII CTCAG 3 cut(s) 165, 330, 573
BseNI ACTGG 2 cut(s) 608, 791
BseXI GCAGC 5 cut(s) 208, 356, 421, 481, 502
BshFI GGCC 2 cut(s) 325, 334
BsiHKAI GWGCWC 1 cut(s) 484
BsiSI CCGG 1 cut(s) 390
BslI CCNNNNNNNGG 1 cut(s) 269
BsmAI GTCTC 2 cut(s) 135, 774
BsnI GGCC 2 cut(s) 325, 334
Bsp1286I GDGCHC 1 cut(s) 484
Bsp143I GATC 2 cut(s) 182, 717
BspACI CCGC 1 cut(s) 882
BspANI GGCC 2 cut(s) 325, 334
BspCNI CTCAG 3 cut(s) 164, 331, 574
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 606
BspMI ACCTGC 1 cut(s) 111
BspPI GGATC 1 cut(s) 190
BsrI ACTGG 2 cut(s) 608, 791
BssECI CCNNGG 2 cut(s) 157, 738
BssMI GATC 2 cut(s) 182, 717
BssNI GRCGYC 2 cut(s) 45, 61
BssT1I CCWWGG 1 cut(s) 157
Bst2UI CCWGG 4 cut(s) 327, 452, 783, 818
Bst4CI ACNGT 2 cut(s) 464, 739
Bst6I CTCTTC 3 cut(s) 78, 617, 705
BstACI GRCGYC 2 cut(s) 45, 61
BstAPI GCANNNNNTGC 1 cut(s) 515
BstC8I GCNNGC 3 cut(s) 219, 332, 336
BstDEI CTNAG 3 cut(s) 151, 339, 582
BstDSI CCRYGG 1 cut(s) 738
BstF5I GGATG 3 cut(s) 278, 447, 475
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 2 cut(s) 185, 720
BstMAI GTCTC 2 cut(s) 135, 774
BstMBI GATC 2 cut(s) 182, 717
BstMWI GCNNNNNNNGC 6 cut(s) 117, 161, 331, 344, 479, 515
BstNI CCWGG 4 cut(s) 327, 452, 783, 818
BstNSI RCATGY 2 cut(s) 298, 666
BstSCI CCNGG 4 cut(s) 325, 450, 781, 816
BstSFI CTRYAG 2 cut(s) 540, 699
BstV1I GCAGC 5 cut(s) 208, 356, 421, 481, 502
BstX2I RGATCY 1 cut(s) 182
BstYI RGATCY 1 cut(s) 182
BsuRI GGCC 2 cut(s) 325, 334
BtgI CCRYGG 1 cut(s) 738
BtsCI GGATG 3 cut(s) 278, 447, 475
BveI ACCTGC 1 cut(s) 111
Cac8I GCNNGC 3 cut(s) 219, 332, 336
CaiI CAGNNNCTG 2 cut(s) 350, 515
CciI TCATGA 1 cut(s) 240
CfoI GCGC 1 cut(s) 190
Cfr13I GGNCC 1 cut(s) 788
CseI GACGC 1 cut(s) 69
CspCI CAANNNNNGTGG 2 cut(s) 766, 801
CviAII CATG 6 cut(s) 241, 295, 394, 577, 663, 721
DdeI CTNAG 3 cut(s) 151, 339, 582
DpnI GATC 2 cut(s) 184, 719
DpnII GATC 2 cut(s) 182, 717
Eam1104I CTCTTC 3 cut(s) 78, 617, 705
EarI CTCTTC 3 cut(s) 78, 617, 705
Eco130I CCWWGG 1 cut(s) 157
Eco147I AGGCCT 1 cut(s) 325
Eco47I GGWCC 1 cut(s) 788
Eco57I CTGAAG 1 cut(s) 842
EcoRI GAATTC 1 cut(s) 287
EcoRII CCWGG 4 cut(s) 325, 450, 781, 816
EcoT14I CCWWGG 1 cut(s) 157
EcoT22I ATGCAT 2 cut(s) 278, 668
ErhI CCWWGG 1 cut(s) 157
FaeI CATG 6 cut(s) 244, 298, 397, 580, 666, 724
FatI CATG 6 cut(s) 240, 294, 393, 576, 662, 720
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 5 cut(s) 222, 345, 435, 495, 516
FokI GGATG 3 cut(s) 285, 454, 482
Fsp4HI GCNGC 5 cut(s) 222, 345, 435, 495, 516
FspBI CTAG 4 cut(s) 138, 191, 837, 869
GlaI GCGC 1 cut(s) 189
GluI GCNGC 5 cut(s) 222, 345, 435, 495, 516
GsuI CTGGAG 1 cut(s) 42
HaeIII GGCC 2 cut(s) 325, 334
HapII CCGG 1 cut(s) 390
HgaI GACGC 1 cut(s) 69
HhaI GCGC 1 cut(s) 190
Hin1I GRCGYC 2 cut(s) 45, 61
Hin1II CATG 6 cut(s) 244, 298, 397, 580, 666, 724
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HincII GTYRAC 1 cut(s) 761
HindII GTYRAC 1 cut(s) 761
HinfI GANTC 6 cut(s) 14, 257, 535, 713, 749, 865
HpaII CCGG 1 cut(s) 390
HphI GGTGA 1 cut(s) 252
Hpy166II GTNNAC 4 cut(s) 105, 448, 761, 788
Hpy188I TCNGA 4 cut(s) 37, 373, 583, 861
Hpy188III TCNNGA 4 cut(s) 21, 241, 254, 869
Hpy8I GTNNAC 4 cut(s) 105, 448, 761, 788
Hpy99I CGWCG 2 cut(s) 50, 211
HpyAV CCTTC 3 cut(s) 258, 400, 554
HpyCH4III ACNGT 2 cut(s) 464, 739
HpyCH4IV ACGT 2 cut(s) 45, 635
HpyCH4V TGCA 5 cut(s) 170, 276, 532, 642, 666
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 161, 331, 344, 479, 515
HpyF3I CTNAG 3 cut(s) 151, 339, 582
HpySE526I ACGT 2 cut(s) 45, 635
Hsp92I GRCGYC 2 cut(s) 45, 61
Hsp92II CATG 6 cut(s) 244, 298, 397, 580, 666, 724
HspAI GCGC 1 cut(s) 188
Kzo9I GATC 2 cut(s) 182, 717
LmnI GCTCC 2 cut(s) 604, 853
Lsp1109I GCAGC 5 cut(s) 208, 356, 421, 481, 502
LweI GCATC 5 cut(s) 40, 179, 263, 460, 481
MaeI CTAG 4 cut(s) 138, 191, 837, 869
MaeII ACGT 2 cut(s) 45, 635
MaeIII GTNAC 3 cut(s) 123, 258, 733
MalI GATC 2 cut(s) 184, 719
MboI GATC 2 cut(s) 182, 717
MboII GAAGA 4 cut(s) 9, 65, 634, 722
MfeI CAATTG 2 cut(s) 282, 315
MflI RGATCY 1 cut(s) 182
MhlI GDGCHC 1 cut(s) 484
MluCI AATT 9 cut(s) 38, 198, 282, 287, 303, 315, 400, 410, 679
MlyI GAGTC 2 cut(s) 266, 722
Mph1103I ATGCAT 2 cut(s) 278, 668
MseI TTAA 2 cut(s) 383, 399
MslI CAYNNNNRTG 1 cut(s) 661
MspA1I CMGCKG 2 cut(s) 338, 884
MspI CCGG 1 cut(s) 390
MspR9I CCNGG 4 cut(s) 327, 452, 783, 818
MunI CAATTG 2 cut(s) 282, 315
MvaI CCWGG 4 cut(s) 327, 452, 783, 818
MwoI GCNNNNNNNGC 6 cut(s) 117, 161, 331, 344, 479, 515
NdeII GATC 2 cut(s) 182, 717
NlaIII CATG 6 cut(s) 244, 298, 397, 580, 666, 724
NlaIV GGNNCC 1 cut(s) 606
NmuCI GTSAC 2 cut(s) 123, 258
NsiI ATGCAT 2 cut(s) 278, 668
NspI RCATGY 2 cut(s) 298, 666
PagI TCATGA 1 cut(s) 240
PceI AGGCCT 1 cut(s) 325
PfeI GAWTC 4 cut(s) 14, 535, 749, 865
PfoI TCCNGGA 1 cut(s) 816
PkrI GCNGC 5 cut(s) 223, 346, 436, 496, 517
PleI GAGTC 2 cut(s) 265, 721
PpsI GAGTC 2 cut(s) 265, 721
PshBI ATTAAT 1 cut(s) 399
PsiI TTATAA 1 cut(s) 675
Psp6I CCWGG 4 cut(s) 325, 450, 781, 816
PspGI CCWGG 4 cut(s) 325, 450, 781, 816
PspN4I GGNNCC 1 cut(s) 606
PspPI GGNCC 1 cut(s) 788
PstNI CAGNNNCTG 2 cut(s) 350, 515
PsuI RGATCY 1 cut(s) 182
PvuII CAGCTG 1 cut(s) 338
RseI CAYNNNNRTG 1 cut(s) 661
SaqAI TTAA 2 cut(s) 383, 399
SatI GCNGC 5 cut(s) 222, 345, 435, 495, 516
Sau3AI GATC 2 cut(s) 182, 717
Sau96I GGNCC 1 cut(s) 788
SchI GAGTC 2 cut(s) 266, 722
ScrFI CCNGG 4 cut(s) 327, 452, 783, 818
SduI GDGCHC 1 cut(s) 484
SfaNI GCATC 5 cut(s) 40, 179, 263, 460, 481
SfcI CTRYAG 2 cut(s) 540, 699
SfiI GGCCNNNNNGGCC 1 cut(s) 331
SinI GGWCC 1 cut(s) 788
SmiMI CAYNNNNRTG 1 cut(s) 661
SmlI CTYRAG 1 cut(s) 252
SmoI CTYRAG 1 cut(s) 252
Sse9I AATT 9 cut(s) 38, 198, 282, 287, 303, 315, 400, 410, 679
SseBI AGGCCT 1 cut(s) 325
SsiI CCGC 1 cut(s) 882
SspMI CTAG 4 cut(s) 138, 191, 837, 869
StuI AGGCCT 1 cut(s) 325
StyD4I CCNGG 4 cut(s) 325, 450, 781, 816
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 2 cut(s) 464, 739
TaiI ACGT 2 cut(s) 48, 638
TaqI TCGA 4 cut(s) 48, 206, 716, 897
TasI AATT 9 cut(s) 38, 198, 282, 287, 303, 315, 400, 410, 679
TfiI GAWTC 4 cut(s) 14, 535, 749, 865
Tru1I TTAA 2 cut(s) 383, 399
Tru9I TTAA 2 cut(s) 383, 399
TseFI GTSAC 2 cut(s) 123, 258
TseI GCWGC 5 cut(s) 221, 344, 434, 494, 515
Tsp45I GTSAC 2 cut(s) 123, 258
TspDTI ATGAA 4 cut(s) 566, 573, 741, 888
VpaK11BI GGWCC 1 cut(s) 788
VspI ATTAAT 1 cut(s) 399
XapI RAATTY 1 cut(s) 287
XbaI TCTAGA 1 cut(s) 868
XceI RCATGY 2 cut(s) 298, 666
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XmiI GTMKAC 1 cut(s) 447
XspI CTAG 4 cut(s) 138, 191, 837, 869
ZraI GACGTC 1 cut(s) 46
Zsp2I ATGCAT 2 cut(s) 278, 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.