Rw0G002840

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00090
OG0007066
Physical Location & Seq
Reverse (-)
48791 .. 51665
2875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G002840.1

Sequence Viewer

Length: 2541 bp
ATGGAGGCCAATAAGTTTGCAACCATGTTGAAGAGGAAAAGTCCCAGGTTCGTTCTAGTTCTAGTCTATGCAGTCCTCGAATGGGTGTTGTTAATACTCCTCCTGCTCAACTCTTTTTTCAGTTACTTGATCACAAAGTTTGCCAACTATTTTGGCCTCAAACCACCTTGTCCCTGGTGTTCTTCTAGAGTTGAGCACATGTTGGAACCACAAAAGTCTTACTCAGATCTTGTATGTGAGATCCATGCCAATGAGATATCTAAACTGGGTTACTGTCTCAATCATAAAAGACTGGCTGAATCACATAGTATGTGTGGGAACTGCTTTTCTTCTCAACCAAATTGCAGAGAAAATTCTACTCAAAGTGGGATGGATTTGATTTCATTGTTGAGTGACAAATATCAGAAGAAACCTTCCATCTGTTCTTGCTGCAGTAAGAGCTTCAACACCAAACTCTCTCCTCTTTCATTGGAGTATGCTCAGAAAGGAGATTCAAATATAGAAGCAGTGCATGATGACAACAATGAAGGCACAACCAAACCTGATAGCGAAAACTGTCGAAATGAAAGTAAGAAACTTGTCCATCAATCTTCCAATGCTACTACCATTGAGTACTGTTGCCTTCAAGAAGATCACTCTCTTGAAACTACCCCCCTTCCTTCTGAGAGTGGTATGGTCTGTGATCTTGGTTGCAGCCTGATTCCTACTGATTTGATTGATTTTTCAACTACAATAGACGAAGGACTCATAAACAGCTTAAAAGATCAGGAACATCATATTAAATTGGAAGATCAACTCTCACGGGAAGCAGCATTACTCATGGTAACTAAGAGTGGAGCAAATACAAGTAATGGAGGACTTAAGAGCTTGGAGATGGCCAAGGGATTTACTGATGCTTCATCTATTGAACAAGTGGAAAAGCGAAATCTAGAGTTGGTTGGCAAGCTATGTGATGATTTGGTCACTGCTCCAGAAGCTCAAACTTCGTTCGTTGACACTGAACAACTAACAGATACCAAAGAACGTAACGATCCACCAGAATGTGAAGAAGAGAGAATTTTGGCCTTCATTTCAAGAAATCTGTCAAACACAAAAGGGTCACACCATGCAGCCGATCAGCTTCAAGCTCACAAGGATTCTTCACTGCCATTCCTTGAAATATCCAATGTGCCAGCTCACAGTGATGTCGGTACAGGGCTTACAGGAAAATCAATGTTGCAAGACAAAACAAGTAGAACTAAAAATGCTCAAGAAGTCAACCATTTTTGCATGTGTTCAGAGCCTAGTGAACCTGAGGAAGAGAGGTTGGTATGCGGACAAAATTGTATAAACACTACCCATTACTTGTACAAAAAGCCGAATGGAAATGGAGAATGTGGGGCAGATGATGGGAGTGTGGCAAGTGTGACGAATGGTGGAGAACCTGTAACAACCATTGAAGAGCTCAAAGCAGCATTGAAGGCTGAAAGTAAAGCTTTAAGTGCTTTATATTCAGAACTAGAAGAAGAGAGAAGTGCATCTGCAGTAGCTGCCAACCAGACTATGGCAATGATAACAAGACTTCAAGAAGAGAAGGCAGCAATGCAGATGGAGGCATTGCAATATCAGAGAATGATGGAAGAACAATCTGAGTATGATCAGGAAGCCTTGCAACTTTTGAATGAGTTGATGAACAAAATGGAGAGGGACAAACAAGAGCTAGAGAAAGAATTGGAAATATATCAGAAGAAAGTCCTGGATTATGAGGAAAAAGAGAAGATAAGGGTCATGAGCAGAATCAAATATGGAAGTATGAGAAGTAGAAATTCTTCTGCTTCCTGCAGCCAATCTTGGGATAGTGATGCGCTATCCATTGATCTCAATTGTGAAGCAAGGGATGAAGATCGTTCTTTCCCTCAAGAATGCAGTAACAACACCAATAATCCAGATGAGGCAGAAGCTCTAAGCTTGGAAGAAATGGCTATGGATTGTGTTAAGCAGATGAGCATTCTTGATGACTCATTGGCAGGCTTCCAGGAAGAGAGGTTGTCAGTTCTGGATCAGCTTAAAGCATTGGAAGATAGGCTAGTAGTGTTGAGTGAAAATGAGAACTGCTCTCAGGATGCTAATTTAGTCGAGAATTGCTCAAACTACAGTACCAAAAACATTGAAGAAAATCATGATTTCAGCAGTCCAGAAGAAAAACAAATATCATATGAGAAAATAACATTGGCTTCCATGGCAAAGAGGCTTCTTCCACTTTTGGGTGTAGCAGCTGAGGATAATGAAACTGAAGCAGGTTTAACAAATGAAAAACAAGTCAGAACCGAATCTATTGCAATGCATAACTCGGTGACCAATTCTGAATTGCATGATACCAGAATAGCCATTGAGGAGGAGGTGGATCAAGTTTATGAGAGGCTACAAGCTCTTGAAAAAAGTAATTTGGAGAAGGGGATGGATCTTCTTCAAGAGAGCTTGCAACATCTCCGGGATTTGAGGGATGTTGAACTCCGCATAAGAAACATTAATGTTCCTGTGGATGAAGCTGCAGGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008092 GO:0017022
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

846

Amino Acids

94.76

Weight (kDa)

4.73

Isoelectric Point (pI)

53.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 481 - 571 1.3e-30 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 2267, 2522
AccB7I CCANNNNNTGG 1 cut(s) 1543
AciI CCGC 2 cut(s) 1314, 2494
AclWI GGATC 5 cut(s) 235, 1025, 2046, 2391, 2448
AcoI YGGCCR 1 cut(s) 876
AcsI RAATTY 3 cut(s) 352, 1056, 1804
AcuI CTGAAG 1 cut(s) 2292
AfaI GTAC 4 cut(s) 614, 1192, 1349, 2137
AfiI CCNNNNNNNGG 4 cut(s) 82, 1543, 1831, 2243
AflII CTTAAG 1 cut(s) 860
AflIII ACRYGT 1 cut(s) 198
AjnI CCWGG 4 cut(s) 44, 173, 1734, 2013
AjuI GAANNNNNNNTTGG 2 cut(s) 2192, 2224
Alw21I GWGCWC 2 cut(s) 198, 1446
Alw26I GTCTC 1 cut(s) 281
AlwI GGATC 5 cut(s) 235, 1025, 2046, 2391, 2448
AlwNI CAGNNNCTG 1 cut(s) 1529
AoxI GGCC 4 cut(s) 6, 154, 876, 1062
ApoI RAATTY 3 cut(s) 352, 1056, 1804
AseI ATTAAT 1 cut(s) 2508
Asp700I GAANNNNTTC 1 cut(s) 1807
AspLEI GCGC 1 cut(s) 1846
AsuC2I CCSGG 1 cut(s) 2471
AsuHPI GGTGA 1 cut(s) 2344
AxyI CCTNAGG 1 cut(s) 1293
BaeI ACNNNNGTAYC 2 cut(s) 2119, 2152
BalI TGGCCA 1 cut(s) 878
BanII GRGCYC 1 cut(s) 1446
BarI GAAGNNNNNNTAC 4 cut(s) 798, 830, 1792, 1824
Bbv12I GWGCWC 2 cut(s) 198, 1446
BbvCI CCTCAGC 1 cut(s) 2256
BccI CCATC 8 cut(s) 364, 425, 591, 868, 1382, 1582, 1609, 2431
BcgI CGANNNNNNTGC 2 cut(s) 2297, 2331
BciT130I CCWGG 4 cut(s) 46, 175, 1736, 2015
BclI TGATCA 2 cut(s) 129, 1636
BcnI CCSGG 1 cut(s) 2471
BcoDI GTCTC 1 cut(s) 281
BfaI CTAG 8 cut(s) 56, 62, 186, 929, 1284, 1499, 1700, 2066
BfmI CTRYAG 5 cut(s) 430, 1521, 1819, 2131, 2529
BfrI CTTAAG 1 cut(s) 860
BfuAI ACCTGC 2 cut(s) 2267, 2522
BglII AGATCT 1 cut(s) 226
BmcAI AGTACT 1 cut(s) 614
Bme1390I CCNGG 5 cut(s) 46, 175, 1736, 2015, 2471
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 5 cut(s) 46, 175, 1736, 2015, 2471
BmrI ACTGGG 1 cut(s) 275
BmsI GCATC 4 cut(s) 883, 1526, 1831, 2092
BmuI ACTGGG 1 cut(s) 275
BplI GAGNNNNNCTC 4 cut(s) 2078, 2110, 2108, 2140
BpmI CTGGAG 1 cut(s) 954
Bpu10I CCTNAGC 1 cut(s) 2256
BpuEI CTTGAG 2 cut(s) 1233, 1881
BpuMI CCSGG 1 cut(s) 2471
BsaBI GATNNNNATC 1 cut(s) 1881
BsaJI CCNNGG 4 cut(s) 44, 173, 879, 2217
BsaXI ACNNNNNCTCC 2 cut(s) 1410, 1440
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 1543, 1831, 2243
Bse1I ACTGG 2 cut(s) 270, 297
Bse21I CCTNAGG 1 cut(s) 1293
Bse3DI GCAATG 4 cut(s) 1554, 1587, 1595, 2325
Bse8I GATNNNNATC 1 cut(s) 1881
BseBI CCWGG 4 cut(s) 46, 175, 1736, 2015
BseDI CCNNGG 4 cut(s) 44, 173, 879, 2217
BseGI GGATG 6 cut(s) 375, 1882, 2107, 2442, 2488, 2527
BseJI GATNNNNATC 1 cut(s) 1881
BseLI CCNNNNNNNGG 4 cut(s) 82, 1543, 1831, 2243
BseMI GCAATG 4 cut(s) 1554, 1587, 1595, 2325
BseMII CTCAG 7 cut(s) 237, 494, 654, 1284, 1620, 2111, 2247
BseNI ACTGG 2 cut(s) 270, 297
BseRI GAGGAG 4 cut(s) 89, 450, 2387, 2390
BshFI GGCC 4 cut(s) 8, 156, 878, 1064
BsiHKAI GWGCWC 2 cut(s) 198, 1446
BsiSI CCGG 1 cut(s) 2470
BslFI GGGAC 3 cut(s) 27, 156, 1700
BslI CCNNNNNNNGG 4 cut(s) 82, 1543, 1831, 2243
BsmAI GTCTC 1 cut(s) 281
BsmFI GGGAC 3 cut(s) 27, 156, 1700
BsmI GAATGC 2 cut(s) 1907, 1986
BsnI GGCC 4 cut(s) 8, 156, 878, 1064
Bsp1286I GDGCHC 2 cut(s) 198, 1446
Bsp1407I TGTACA 1 cut(s) 1347
Bsp19I CCATGG 1 cut(s) 2217
BspACI CCGC 2 cut(s) 1314, 2494
BspANI GGCC 4 cut(s) 8, 156, 878, 1064
BspCNI CTCAG 7 cut(s) 236, 493, 655, 1285, 1621, 2110, 2248
BspHI TCATGA 2 cut(s) 1767, 2158
BspLI GGNNCC 1 cut(s) 207
BspMAI CTGCAG 4 cut(s) 434, 1525, 1823, 2533
BspMI ACCTGC 2 cut(s) 2267, 2522
BspPI GGATC 5 cut(s) 235, 1025, 2046, 2391, 2448
BspQI GCTCTTC 1 cut(s) 1434
BspTI CTTAAG 1 cut(s) 860
BsrDI GCAATG 4 cut(s) 1554, 1587, 1595, 2325
BsrGI TGTACA 1 cut(s) 1347
BsrI ACTGG 2 cut(s) 270, 297
BssECI CCNNGG 4 cut(s) 44, 173, 879, 2217
BssT1I CCWWGG 2 cut(s) 879, 2217
Bst2UI CCWGG 4 cut(s) 46, 175, 1736, 2015
Bst4CI ACNGT 5 cut(s) 275, 557, 617, 1181, 2135
Bst6I CTCTTC 7 cut(s) 26, 1044, 1293, 1434, 1500, 1563, 2013
BstAFI CTTAAG 1 cut(s) 860
BstAPI GCANNNNNTGC 1 cut(s) 1529
BstAUI TGTACA 1 cut(s) 1347
BstC8I GCNNGC 4 cut(s) 944, 1173, 2008, 2459
BstDEI CTNAG 9 cut(s) 223, 480, 663, 828, 1293, 1629, 1943, 2097, 2256
BstDSI CCRYGG 1 cut(s) 2217
BstEII GGTNACC 1 cut(s) 2332
BstF5I GGATG 6 cut(s) 375, 1882, 2107, 2442, 2488, 2527
BstHHI GCGC 1 cut(s) 1846
BstMAI GTCTC 1 cut(s) 281
BstMWI GCNNNNNNNGC 6 cut(s) 438, 974, 1460, 1481, 1529, 2219
BstNI CCWGG 4 cut(s) 46, 175, 1736, 2015
BstNSI RCATGY 2 cut(s) 202, 1273
BstPI GGTNACC 1 cut(s) 2332
BstSCI CCNGG 5 cut(s) 44, 173, 1734, 2013, 2469
BstSFI CTRYAG 5 cut(s) 430, 1521, 1819, 2131, 2529
BstX2I RGATCY 3 cut(s) 226, 240, 2440
BstYI RGATCY 3 cut(s) 226, 240, 2440
Bsu36I CCTNAGG 1 cut(s) 1293
BsuRI GGCC 4 cut(s) 8, 156, 878, 1064
BtgI CCRYGG 1 cut(s) 2217
BtsCI GGATG 6 cut(s) 375, 1882, 2107, 2442, 2488, 2527
BtsI GCAGTG 3 cut(s) 513, 963, 1142
BtsIMutI CAGTG 5 cut(s) 513, 963, 996, 1142, 1186
BveI ACCTGC 2 cut(s) 2267, 2522
Cac8I GCNNGC 4 cut(s) 944, 1173, 2008, 2459
CaiI CAGNNNCTG 1 cut(s) 1529
CciI TCATGA 2 cut(s) 1767, 2158
CfoI GCGC 1 cut(s) 1846
Csp6I GTAC 4 cut(s) 613, 1191, 1348, 2136
CviQI GTAC 4 cut(s) 613, 1191, 1348, 2136
DdeI CTNAG 9 cut(s) 223, 480, 663, 828, 1293, 1629, 1943, 2097, 2256
EaeI YGGCCR 1 cut(s) 876
Eam1104I CTCTTC 7 cut(s) 26, 1044, 1293, 1434, 1500, 1563, 2013
EarI CTCTTC 7 cut(s) 26, 1044, 1293, 1434, 1500, 1563, 2013
Ecl136II GAGCTC 1 cut(s) 1444
Eco130I CCWWGG 2 cut(s) 879, 2217
Eco24I GRGCYC 1 cut(s) 1446
Eco32I GATATC 1 cut(s) 258
Eco53kI GAGCTC 1 cut(s) 1444
Eco57I CTGAAG 1 cut(s) 2292
Eco81I CCTNAGG 1 cut(s) 1293
Eco91I GGTNACC 1 cut(s) 2332
EcoICRI GAGCTC 1 cut(s) 1444
EcoO65I GGTNACC 1 cut(s) 2332
EcoRII CCWGG 4 cut(s) 44, 173, 1734, 2013
EcoRV GATATC 1 cut(s) 258
EcoT14I CCWWGG 2 cut(s) 879, 2217
EcoT22I ATGCAT 1 cut(s) 2325
EcoT38I GRGCYC 1 cut(s) 1446
ErhI CCWWGG 2 cut(s) 879, 2217
FalI AAGNNNNNCTT 2 cut(s) 1459, 1491
FaqI GGGAC 3 cut(s) 27, 156, 1700
FauNDI CATATG 1 cut(s) 2194
FbaI TGATCA 2 cut(s) 129, 1636
FokI GGATG 6 cut(s) 382, 1889, 2114, 2449, 2495, 2534
FriOI GRGCYC 1 cut(s) 1446
FspBI CTAG 8 cut(s) 56, 62, 186, 929, 1284, 1499, 1700, 2066
GlaI GCGC 1 cut(s) 1845
GsuI CTGGAG 1 cut(s) 954
HaeIII GGCC 4 cut(s) 8, 156, 878, 1064
HapII CCGG 1 cut(s) 2470
HhaI GCGC 1 cut(s) 1846
Hin6I GCGC 1 cut(s) 1844
HinP1I GCGC 1 cut(s) 1844
HincII GTYRAC 2 cut(s) 994, 1258
HindII GTYRAC 2 cut(s) 994, 1258
HindIII AAGCTT 2 cut(s) 1473, 1945
HinfI GANTC 8 cut(s) 299, 491, 700, 744, 1136, 1776, 1997, 2309
HpaII CCGG 1 cut(s) 2470
HphI GGTGA 1 cut(s) 2344
Hpy166II GTNNAC 3 cut(s) 994, 1258, 1289
Hpy8I GTNNAC 3 cut(s) 994, 1258, 1289
HpyCH4III ACNGT 5 cut(s) 275, 557, 617, 1181, 2135
HpyCH4IV ACGT 1 cut(s) 1024
HpyF10VI GCNNNNNNNGC 6 cut(s) 438, 974, 1460, 1481, 1529, 2219
HpyF3I CTNAG 9 cut(s) 223, 480, 663, 828, 1293, 1629, 1943, 2097, 2256
HpySE526I ACGT 1 cut(s) 1024
HspAI GCGC 1 cut(s) 1844
Ksp22I TGATCA 2 cut(s) 129, 1636
LguI GCTCTTC 1 cut(s) 1434
LmnI GCTCC 2 cut(s) 836, 973
LweI GCATC 4 cut(s) 883, 1526, 1831, 2092
MaeI CTAG 8 cut(s) 56, 62, 186, 929, 1284, 1499, 1700, 2066
MaeII ACGT 1 cut(s) 1024
MfeI CAATTG 1 cut(s) 1861
MflI RGATCY 3 cut(s) 226, 240, 2440
MhlI GDGCHC 2 cut(s) 198, 1446
MlsI TGGCCA 1 cut(s) 878
MluNI TGGCCA 1 cut(s) 878
MlyI GAGTC 2 cut(s) 738, 1991
MmeI TCCRAC 1 cut(s) 183
Mox20I TGGCCA 1 cut(s) 878
Mph1103I ATGCAT 1 cut(s) 2325
MroXI GAANNNNTTC 1 cut(s) 1807
MscI TGGCCA 1 cut(s) 878
MseI TTAA 9 cut(s) 92, 758, 780, 861, 1478, 1974, 2046, 2282, 2508
MslI CAYNNNNRTG 3 cut(s) 249, 1039, 1182
Msp20I TGGCCA 1 cut(s) 878
MspA1I CMGCKG 1 cut(s) 2255
MspCI CTTAAG 1 cut(s) 860
MspI CCGG 1 cut(s) 2470
MspR9I CCNGG 5 cut(s) 46, 175, 1736, 2015, 2471
MunI CAATTG 1 cut(s) 1861
Mva1269I GAATGC 2 cut(s) 1907, 1986
MvaI CCWGG 4 cut(s) 46, 175, 1736, 2015
MwoI GCNNNNNNNGC 6 cut(s) 438, 974, 1460, 1481, 1529, 2219
NciI CCSGG 1 cut(s) 2471
NcoI CCATGG 1 cut(s) 2217
NdeI CATATG 1 cut(s) 2194
NlaIV GGNNCC 1 cut(s) 207
NmuCI GTSAC 5 cut(s) 392, 961, 1098, 1405, 2332
NsiI ATGCAT 1 cut(s) 2325
NspI RCATGY 2 cut(s) 202, 1273
PagI TCATGA 2 cut(s) 1767, 2158
PciI ACATGT 1 cut(s) 198
PciSI GCTCTTC 1 cut(s) 1434
PctI GAATGC 2 cut(s) 1907, 1986
PdmI GAANNNNTTC 1 cut(s) 1807
PfeI GAWTC 6 cut(s) 299, 491, 700, 1136, 1776, 2309
PflMI CCANNNNNTGG 1 cut(s) 1543
PfoI TCCNGGA 3 cut(s) 1734, 2013, 2469
PleI GAGTC 2 cut(s) 738, 1991
PpsI GAGTC 2 cut(s) 738, 1991
PscI ACATGT 1 cut(s) 198
PshBI ATTAAT 1 cut(s) 2508
Psp124BI GAGCTC 1 cut(s) 1446
Psp6I CCWGG 4 cut(s) 44, 173, 1734, 2013
PspEI GGTNACC 1 cut(s) 2332
PspGI CCWGG 4 cut(s) 44, 173, 1734, 2013
PspN4I GGNNCC 1 cut(s) 207
PstI CTGCAG 4 cut(s) 434, 1525, 1823, 2533
PstNI CAGNNNCTG 1 cut(s) 1529
PsuI RGATCY 3 cut(s) 226, 240, 2440
PvuII CAGCTG 1 cut(s) 2255
RsaI GTAC 4 cut(s) 614, 1192, 1349, 2137
RsaNI GTAC 4 cut(s) 613, 1191, 1348, 2136
RseI CAYNNNNRTG 3 cut(s) 249, 1039, 1182
SacI GAGCTC 1 cut(s) 1446
SapI GCTCTTC 1 cut(s) 1434
SaqAI TTAA 9 cut(s) 92, 758, 780, 861, 1478, 1974, 2046, 2282, 2508
ScaI AGTACT 1 cut(s) 614
SchI GAGTC 2 cut(s) 738, 1991
ScrFI CCNGG 5 cut(s) 46, 175, 1736, 2015, 2471
SduI GDGCHC 2 cut(s) 198, 1446
SfaNI GCATC 4 cut(s) 883, 1526, 1831, 2092
SfcI CTRYAG 5 cut(s) 430, 1521, 1819, 2131, 2529
SmiMI CAYNNNNRTG 3 cut(s) 249, 1039, 1182
SmlI CTYRAG 3 cut(s) 860, 1248, 1896
SmoI CTYRAG 3 cut(s) 860, 1248, 1896
SsiI CCGC 2 cut(s) 1314, 2494
SspMI CTAG 8 cut(s) 56, 62, 186, 929, 1284, 1499, 1700, 2066
SstI GAGCTC 1 cut(s) 1446
StyD4I CCNGG 5 cut(s) 44, 173, 1734, 2013, 2469
StyI CCWWGG 2 cut(s) 879, 2217
TaaI ACNGT 5 cut(s) 275, 557, 617, 1181, 2135
TaiI ACGT 1 cut(s) 1027
TaqI TCGA 3 cut(s) 78, 559, 2115
TatI WGTACW 2 cut(s) 612, 1347
TfiI GAWTC 6 cut(s) 299, 491, 700, 1136, 1776, 2309
Tru1I TTAA 9 cut(s) 92, 758, 780, 861, 1478, 1974, 2046, 2282, 2508
Tru9I TTAA 9 cut(s) 92, 758, 780, 861, 1478, 1974, 2046, 2282, 2508
TscAI CASTG 5 cut(s) 513, 970, 1003, 1149, 1186
TseFI GTSAC 5 cut(s) 392, 961, 1098, 1405, 2332
Tsp45I GTSAC 5 cut(s) 392, 961, 1098, 1405, 2332
TspRI CASTG 5 cut(s) 513, 970, 1003, 1149, 1186
Van91I CCANNNNNTGG 1 cut(s) 1543
Vha464I CTTAAG 1 cut(s) 860
VspI ATTAAT 1 cut(s) 2508
XapI RAATTY 3 cut(s) 352, 1056, 1804
XbaI TCTAGA 2 cut(s) 185, 928
XceI RCATGY 2 cut(s) 202, 1273
XcmI CCANNNNNNNNNTGG 2 cut(s) 171, 1540
XmnI GAANNNNTTC 1 cut(s) 1807
XspI CTAG 8 cut(s) 56, 62, 186, 929, 1284, 1499, 1700, 2066
ZrmI AGTACT 1 cut(s) 614
Zsp2I ATGCAT 1 cut(s) 2325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.