RchiOBHm_Chr3g0493141

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007066
Physical Location & Seq
Forward (+)
40270511 .. 40274221
3711 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45596

Sequence Viewer

Length: 2478 bp
ATGGAGGCCAATAAGTTTGCAACCATGTTGAAGAGGAAAAGTCCCAGGTTCGTTCTAGTTCTAGTCTATGCAGTCCTCGAATGGGTGCTGTTAATACTCCTCCTGCTCAACTCTTTTTTCAGTTACTTGATCACAAAGTTTGCCAACTATTTTGGCCTCAAACCACCTTGTCCCTGGTGTTCTTCTAGAGTTGAGCACATGTTGGAACCACAAAAGTCTTACTCAGATCTTGTATGTGAGATCCATGCCAATGAGATATCTAAACTGGGTTACTGTCTCAATCATAAAAGACTGGCTGAATCACATAGTATGTGTGGGAACTGCTTTTCTTCTCAACCAAATTGCAGAGAAAATTCTACTCAAAGTAGGATGGATTTGATTTCATTGCTGGGTGACAAATATCAGAAGAAACCTTCCATCTGTTCTTGCTGCAGTAAGAGCTTCAACACCAAACTCTCTCCTCTTTCATTGGAGTATGCTCACAAAGGAGATTCAAATATAGAAGCAGTGCATGATGACAACAATGAAGGCACAACCAAACCTGATAGCATGTCAGTCCACAGCGAAAATGGCTATGAGATGGAGACAACTTCTAGCTTAAGAGACACTACTGCAGAAGAAAACTGTCGAAATGAAAACGAAGGACTCATAAACAGCTTAAAAGATCAGGAACATCATGATCATGAACAAGGAAGTTTTGATTCCAAGTCAAAGATTGAATTGGAAGATCAACTCTCACGGGAAGCAGCATTACTCATGGTAACTAAGAGTGGAGCAAATACAAGTAATGGAGGACTTAAGAGCTTGGAGATGGCCAAGGGATTTACTGATGCTTCATCTATTGAACAAGTGGAAAAGCGAAATCTAGAGTTGGTTGGCATGCTATGTGATGATTTGGTCACTGCTCCAGAAGCTCAAACTTCGTTCGTTGACACTGAACAACTAACAAATACCAAAGAACCTAACGATCCACCAGAATGTGAAGAAGAGAGAATTTTGGCCGTCATTTCAAGAAATCTGTCAAACACAAAAGGGTCACACCATGCAGCCGATCAGCCTCAAGCTCACAAGGATTCTTCACTGCCATTCCTTGAAATATCCAATGTGCCAGCTCACAGTGATGTCGGTACAGGGCCTACAGGAAAATCAGTGTTGCAAGACAAAACAAGTAGAACTAAAAATGCTCAAGAAGTCAACCATTTTTGCATGTGTTCAGAGCCTAGTGAACCTGAGGAAGAGAGGTTGGTATGCGGACAAAATTGTATAAACACTATCCATTACTTGTACAAAAAGCCGAATGGAAATGGAGAATGTGGGGCAGATGATGGGAGTGTGGCAAGTGTGACGAATGGTGGAGAACCTGTAACAACCGTTGAAGAGCTCAAAGCAGCATTGAAGGCTGAAAGTAAAGCTTTAAGTGCTTTATATTCAGAACTAGAGGAAGAGAGAAGTGCGTCTGCAGTAGCTGCCAACCAGACTATGGCTATGATAACAAGGCTTCAAGAAGAGAAGGCAGCAATGCAGATGGAGGCATTGCAATATCAGAGAATGATGGAAGAACAATCTGAGTATGATCAGGAAGCCTTGCAACTTTTGAATGAGTTGATGAACAAAATGGAGAGGGAAAAACAAGAGCTAGAGAAAGAATTGGAAATATATCAGAAGAAAGTCCTGGATTATGAGGAAAAAGAGAAGATAAGGGTCATGAGCAGAATCAAATATGGAAGTATGAGAAGTAGAAATTCTTCTGCTTCCTGCAGCCAATCTTGGGATAGTGATGCGCTATCCATTGATCTCAATTGTGAAGCAAGGGATGAAGATAGTTCTTTCCCTCAAGAATGCAGTAACAACACCAATAATCCAGATGATGCAGAAGCTCTAAGCTTGGAAGAAATGGCTCTGGATTGTGTTAAGCAGATGAGCATTCTTGATAACTCATTGGCAGGCTTCCAGGAAGAGAGGTTGTCAGTTCTGGATCAGCTTAAAGCATTGGAAGATAGGCTAGTAGTGTTGAGTGAAAATGAGAACTGCTCTCAGGATGCTAATTTAGTCGAGAATTGCTCAAACTACAGTACCAAAAACATTGAAGAAAATCATGATTTCAGCAGTCCAGAAGAAAAACAAATATCAGATGAGAAAATAACATTGGCTTCCATGGCAAAGAGGCTTCTTCCACTTTTGGGTGTAGCAGCTGAGGATAATGAAACTGAAGCAGGTTTAACAAATGAAAAACAAGTCAGAACTGAATCTATTGCAATGCATAACTCGGTGACGAATTCTGAATTGCATGATACCAGAATAGCCATTGAGGAGGAGGTGGATCATGTTTATGAGAGGCTACAAGCTCTTGAAAAAAGTAATTTGGAGAAGGGGATGGATCTTCTTCAAGAGAGCTTGCAACATCTCCGGGATTTGAGGGATGTTGAACTCTGCATAAGAAACATTAATGTTCCTGTGGATGAAGCTGCAGGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008092 GO:0017022
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

825

Amino Acids

92.33

Weight (kDa)

4.73

Isoelectric Point (pI)

52.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 460 - 550 9.2e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 2204, 2459
AccB7I CCANNNNNTGG 1 cut(s) 1480
AciI CCGC 1 cut(s) 1251
AclWI GGATC 5 cut(s) 235, 962, 1983, 2328, 2385
AcoI YGGCCR 2 cut(s) 813, 999
AcsI RAATTY 4 cut(s) 352, 993, 1741, 2275
AcuI CTGAAG 1 cut(s) 2229
AfaI GTAC 3 cut(s) 1129, 1286, 2074
AfiI CCNNNNNNNGG 4 cut(s) 82, 1480, 1768, 2180
AflII CTTAAG 2 cut(s) 598, 797
AflIII ACRYGT 1 cut(s) 198
AjnI CCWGG 4 cut(s) 44, 173, 1671, 1950
AjuI GAANNNNNNNTTGG 2 cut(s) 2129, 2161
Alw21I GWGCWC 2 cut(s) 198, 1383
Alw26I GTCTC 3 cut(s) 281, 578, 597
AlwI GGATC 5 cut(s) 235, 962, 1983, 2328, 2385
AlwNI CAGNNNCTG 1 cut(s) 1466
AoxI GGCC 5 cut(s) 6, 154, 813, 999, 1133
ApeKI GCWGC 9 cut(s) 429, 746, 1046, 1388, 1466, 1514, 1758, 2189, 2465
ApoI RAATTY 4 cut(s) 352, 993, 1741, 2275
AseI ATTAAT 1 cut(s) 2445
Asp700I GAANNNNTTC 1 cut(s) 1744
AspLEI GCGC 1 cut(s) 1783
AspS9I GGNCC 1 cut(s) 1133
AsuC2I CCSGG 1 cut(s) 2408
AsuHPI GGTGA 2 cut(s) 404, 2281
AxyI CCTNAGG 1 cut(s) 1230
BaeI ACNNNNGTAYC 2 cut(s) 2056, 2089
BalI TGGCCA 1 cut(s) 815
BanII GRGCYC 1 cut(s) 1383
BarI GAAGNNNNNNTAC 4 cut(s) 735, 767, 1729, 1761
Bbv12I GWGCWC 2 cut(s) 198, 1383
BbvCI CCTCAGC 1 cut(s) 2193
BbvI GCAGC 9 cut(s) 416, 758, 1058, 1400, 1453, 1526, 1770, 2201, 2452
BccI CCATC 8 cut(s) 364, 425, 574, 805, 1319, 1519, 1546, 2368
BceAI ACGGC 1 cut(s) 986
BciT130I CCWGG 4 cut(s) 46, 175, 1673, 1952
BclI TGATCA 3 cut(s) 129, 679, 1573
BcnI CCSGG 1 cut(s) 2408
BcoDI GTCTC 3 cut(s) 281, 578, 597
BfaI CTAG 9 cut(s) 56, 62, 186, 594, 866, 1221, 1436, 1637, 2003
BfmI CTRYAG 7 cut(s) 430, 612, 1137, 1458, 1756, 2068, 2466
BfrI CTTAAG 2 cut(s) 598, 797
BfuAI ACCTGC 2 cut(s) 2204, 2459
BglII AGATCT 1 cut(s) 226
BisI GCNGC 9 cut(s) 430, 747, 1047, 1389, 1467, 1515, 1759, 2190, 2466
BlsI GCNGC 9 cut(s) 431, 748, 1048, 1390, 1468, 1516, 1760, 2191, 2467
Bme1390I CCNGG 5 cut(s) 46, 175, 1673, 1952, 2408
BmgT120I GGNCC 1 cut(s) 1133
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 5 cut(s) 46, 175, 1673, 1952, 2408
BmrI ACTGGG 1 cut(s) 275
BmsI GCATC 4 cut(s) 820, 1768, 1858, 2029
BmuI ACTGGG 1 cut(s) 275
BplI GAGNNNNNCTC 4 cut(s) 2015, 2047, 2045, 2077
BpmI CTGGAG 1 cut(s) 891
Bpu10I CCTNAGC 1 cut(s) 2193
BpuEI CTTGAG 3 cut(s) 1044, 1170, 1818
BpuMI CCSGG 1 cut(s) 2408
BsaJI CCNNGG 4 cut(s) 44, 173, 816, 2154
BsaXI ACNNNNNCTCC 2 cut(s) 1347, 1377
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 1480, 1768, 2180
Bse1I ACTGG 2 cut(s) 270, 297
Bse21I CCTNAGG 1 cut(s) 1230
Bse3DI GCAATG 4 cut(s) 383, 1524, 1532, 2262
BseBI CCWGG 4 cut(s) 46, 175, 1673, 1952
BseDI CCNNGG 4 cut(s) 44, 173, 816, 2154
BseGI GGATG 6 cut(s) 375, 1819, 2044, 2379, 2425, 2464
BseLI CCNNNNNNNGG 4 cut(s) 82, 1480, 1768, 2180
BseMI GCAATG 4 cut(s) 383, 1524, 1532, 2262
BseMII CTCAG 5 cut(s) 237, 1221, 1557, 2048, 2184
BseNI ACTGG 2 cut(s) 270, 297
BseRI GAGGAG 4 cut(s) 89, 450, 2324, 2327
BseXI GCAGC 9 cut(s) 416, 758, 1058, 1400, 1453, 1526, 1770, 2201, 2452
BseYI CCCAGC 1 cut(s) 388
BshFI GGCC 5 cut(s) 8, 156, 815, 1001, 1135
BsiHKAI GWGCWC 2 cut(s) 198, 1383
BsiSI CCGG 1 cut(s) 2407
BslFI GGGAC 2 cut(s) 27, 156
BslI CCNNNNNNNGG 4 cut(s) 82, 1480, 1768, 2180
BsmAI GTCTC 3 cut(s) 281, 578, 597
BsmFI GGGAC 2 cut(s) 27, 156
BsmI GAATGC 2 cut(s) 1844, 1923
BsnI GGCC 5 cut(s) 8, 156, 815, 1001, 1135
Bsp1286I GDGCHC 2 cut(s) 198, 1383
Bsp1407I TGTACA 1 cut(s) 1284
Bsp19I CCATGG 1 cut(s) 2154
BspACI CCGC 1 cut(s) 1251
BspANI GGCC 5 cut(s) 8, 156, 815, 1001, 1135
BspCNI CTCAG 5 cut(s) 236, 1222, 1558, 2047, 2185
BspHI TCATGA 4 cut(s) 676, 682, 1704, 2095
BspLI GGNNCC 1 cut(s) 207
BspMAI CTGCAG 5 cut(s) 434, 616, 1462, 1760, 2470
BspMI ACCTGC 2 cut(s) 2204, 2459
BspPI GGATC 5 cut(s) 235, 962, 1983, 2328, 2385
BspQI GCTCTTC 1 cut(s) 1371
BspTI CTTAAG 2 cut(s) 598, 797
BsrDI GCAATG 4 cut(s) 383, 1524, 1532, 2262
BsrGI TGTACA 1 cut(s) 1284
BsrI ACTGG 2 cut(s) 270, 297
BssECI CCNNGG 4 cut(s) 44, 173, 816, 2154
BssT1I CCWWGG 2 cut(s) 816, 2154
Bst2UI CCWGG 4 cut(s) 46, 175, 1673, 1952
Bst4CI ACNGT 5 cut(s) 275, 626, 1118, 1372, 2072
Bst6I CTCTTC 7 cut(s) 26, 981, 1230, 1371, 1437, 1500, 1950
BstAFI CTTAAG 2 cut(s) 598, 797
BstAPI GCANNNNNTGC 1 cut(s) 1466
BstAUI TGTACA 1 cut(s) 1284
BstC8I GCNNGC 4 cut(s) 881, 1110, 1945, 2396
BstDEI CTNAG 7 cut(s) 223, 765, 1230, 1566, 1880, 2034, 2193
BstDSI CCRYGG 1 cut(s) 2154
BstF5I GGATG 6 cut(s) 375, 1819, 2044, 2379, 2425, 2464
BstHHI GCGC 1 cut(s) 1783
BstMAI GTCTC 3 cut(s) 281, 578, 597
BstMWI GCNNNNNNNGC 7 cut(s) 438, 570, 911, 1397, 1418, 1466, 2156
BstNI CCWGG 4 cut(s) 46, 175, 1673, 1952
BstNSI RCATGY 4 cut(s) 202, 553, 883, 1210
BstSCI CCNGG 5 cut(s) 44, 173, 1671, 1950, 2406
BstSFI CTRYAG 7 cut(s) 430, 612, 1137, 1458, 1756, 2068, 2466
BstV1I GCAGC 9 cut(s) 416, 758, 1058, 1400, 1453, 1526, 1770, 2201, 2452
BstX2I RGATCY 3 cut(s) 226, 240, 2377
BstYI RGATCY 3 cut(s) 226, 240, 2377
Bsu36I CCTNAGG 1 cut(s) 1230
BsuRI GGCC 5 cut(s) 8, 156, 815, 1001, 1135
BtgI CCRYGG 1 cut(s) 2154
BtsCI GGATG 6 cut(s) 375, 1819, 2044, 2379, 2425, 2464
BtsI GCAGTG 3 cut(s) 513, 900, 1079
BtsIMutI CAGTG 6 cut(s) 513, 900, 933, 1079, 1123, 1155
BveI ACCTGC 2 cut(s) 2204, 2459
Cac8I GCNNGC 4 cut(s) 881, 1110, 1945, 2396
CaiI CAGNNNCTG 1 cut(s) 1466
CciI TCATGA 4 cut(s) 676, 682, 1704, 2095
CfoI GCGC 1 cut(s) 1783
Cfr13I GGNCC 1 cut(s) 1133
CseI GACGC 1 cut(s) 1443
Csp6I GTAC 3 cut(s) 1128, 1285, 2073
CviQI GTAC 3 cut(s) 1128, 1285, 2073
DdeI CTNAG 7 cut(s) 223, 765, 1230, 1566, 1880, 2034, 2193
EaeI YGGCCR 2 cut(s) 813, 999
Eam1104I CTCTTC 7 cut(s) 26, 981, 1230, 1371, 1437, 1500, 1950
EarI CTCTTC 7 cut(s) 26, 981, 1230, 1371, 1437, 1500, 1950
Ecl136II GAGCTC 1 cut(s) 1381
Eco130I CCWWGG 2 cut(s) 816, 2154
Eco24I GRGCYC 1 cut(s) 1383
Eco32I GATATC 1 cut(s) 258
Eco53kI GAGCTC 1 cut(s) 1381
Eco57I CTGAAG 1 cut(s) 2229
Eco81I CCTNAGG 1 cut(s) 1230
EcoICRI GAGCTC 1 cut(s) 1381
EcoO109I RGGNCCY 1 cut(s) 1133
EcoRI GAATTC 1 cut(s) 2275
EcoRII CCWGG 4 cut(s) 44, 173, 1671, 1950
EcoRV GATATC 1 cut(s) 258
EcoT14I CCWWGG 2 cut(s) 816, 2154
EcoT22I ATGCAT 1 cut(s) 2262
EcoT38I GRGCYC 1 cut(s) 1383
ErhI CCWWGG 2 cut(s) 816, 2154
FalI AAGNNNNNCTT 2 cut(s) 1396, 1428
FaqI GGGAC 2 cut(s) 27, 156
FbaI TGATCA 3 cut(s) 129, 679, 1573
Fnu4HI GCNGC 9 cut(s) 430, 747, 1047, 1389, 1467, 1515, 1759, 2190, 2466
FokI GGATG 6 cut(s) 382, 1826, 2051, 2386, 2432, 2471
FriOI GRGCYC 1 cut(s) 1383
Fsp4HI GCNGC 9 cut(s) 430, 747, 1047, 1389, 1467, 1515, 1759, 2190, 2466
FspBI CTAG 9 cut(s) 56, 62, 186, 594, 866, 1221, 1436, 1637, 2003
GlaI GCGC 1 cut(s) 1782
GluI GCNGC 9 cut(s) 430, 747, 1047, 1389, 1467, 1515, 1759, 2190, 2466
GsaI CCCAGC 1 cut(s) 392
GsuI CTGGAG 1 cut(s) 891
HaeIII GGCC 5 cut(s) 8, 156, 815, 1001, 1135
HapII CCGG 1 cut(s) 2407
HgaI GACGC 1 cut(s) 1443
HhaI GCGC 1 cut(s) 1783
Hin6I GCGC 1 cut(s) 1781
HinP1I GCGC 1 cut(s) 1781
HincII GTYRAC 2 cut(s) 931, 1195
HindII GTYRAC 2 cut(s) 931, 1195
HindIII AAGCTT 2 cut(s) 1410, 1882
HinfI GANTC 7 cut(s) 299, 491, 645, 701, 1073, 1713, 2246
HpaII CCGG 1 cut(s) 2407
HphI GGTGA 2 cut(s) 404, 2281
Hpy166II GTNNAC 4 cut(s) 559, 931, 1195, 1226
Hpy8I GTNNAC 4 cut(s) 559, 931, 1195, 1226
HpyAV CCTTC 6 cut(s) 423, 521, 635, 1390, 1504, 2362
HpyCH4III ACNGT 5 cut(s) 275, 626, 1118, 1372, 2072
HpyF10VI GCNNNNNNNGC 7 cut(s) 438, 570, 911, 1397, 1418, 1466, 2156
HpyF3I CTNAG 7 cut(s) 223, 765, 1230, 1566, 1880, 2034, 2193
HspAI GCGC 1 cut(s) 1781
Ksp22I TGATCA 3 cut(s) 129, 679, 1573
LguI GCTCTTC 1 cut(s) 1371
LmnI GCTCC 2 cut(s) 773, 910
Lsp1109I GCAGC 9 cut(s) 416, 758, 1058, 1400, 1453, 1526, 1770, 2201, 2452
LweI GCATC 4 cut(s) 820, 1768, 1858, 2029
MaeI CTAG 9 cut(s) 56, 62, 186, 594, 866, 1221, 1436, 1637, 2003
MfeI CAATTG 1 cut(s) 1798
MflI RGATCY 3 cut(s) 226, 240, 2377
MhlI GDGCHC 2 cut(s) 198, 1383
MlsI TGGCCA 1 cut(s) 815
MluNI TGGCCA 1 cut(s) 815
MlyI GAGTC 1 cut(s) 639
MmeI TCCRAC 1 cut(s) 183
Mox20I TGGCCA 1 cut(s) 815
Mph1103I ATGCAT 1 cut(s) 2262
MroXI GAANNNNTTC 1 cut(s) 1744
MscI TGGCCA 1 cut(s) 815
MseI TTAA 9 cut(s) 92, 599, 659, 798, 1415, 1911, 1983, 2219, 2445
MslI CAYNNNNRTG 5 cut(s) 249, 681, 976, 1119, 2328
Msp20I TGGCCA 1 cut(s) 815
MspA1I CMGCKG 1 cut(s) 2192
MspCI CTTAAG 2 cut(s) 598, 797
MspI CCGG 1 cut(s) 2407
MspR9I CCNGG 5 cut(s) 46, 175, 1673, 1952, 2408
MunI CAATTG 1 cut(s) 1798
Mva1269I GAATGC 2 cut(s) 1844, 1923
MvaI CCWGG 4 cut(s) 46, 175, 1673, 1952
MwoI GCNNNNNNNGC 7 cut(s) 438, 570, 911, 1397, 1418, 1466, 2156
NciI CCSGG 1 cut(s) 2408
NcoI CCATGG 1 cut(s) 2154
NlaIV GGNNCC 1 cut(s) 207
NmuCI GTSAC 5 cut(s) 392, 898, 1035, 1342, 2269
NsiI ATGCAT 1 cut(s) 2262
NspI RCATGY 4 cut(s) 202, 553, 883, 1210
PaeI GCATGC 1 cut(s) 883
PagI TCATGA 4 cut(s) 676, 682, 1704, 2095
PciI ACATGT 1 cut(s) 198
PciSI GCTCTTC 1 cut(s) 1371
PctI GAATGC 2 cut(s) 1844, 1923
PdmI GAANNNNTTC 1 cut(s) 1744
PfeI GAWTC 6 cut(s) 299, 491, 701, 1073, 1713, 2246
PflMI CCANNNNNTGG 1 cut(s) 1480
PfoI TCCNGGA 3 cut(s) 1671, 1950, 2406
PkrI GCNGC 9 cut(s) 431, 748, 1048, 1390, 1468, 1516, 1760, 2191, 2467
PleI GAGTC 1 cut(s) 639
PpsI GAGTC 1 cut(s) 639
PscI ACATGT 1 cut(s) 198
PshBI ATTAAT 1 cut(s) 2445
Psp124BI GAGCTC 1 cut(s) 1383
Psp6I CCWGG 4 cut(s) 44, 173, 1671, 1950
PspFI CCCAGC 1 cut(s) 388
PspGI CCWGG 4 cut(s) 44, 173, 1671, 1950
PspN4I GGNNCC 1 cut(s) 207
PspPI GGNCC 1 cut(s) 1133
PstI CTGCAG 5 cut(s) 434, 616, 1462, 1760, 2470
PstNI CAGNNNCTG 1 cut(s) 1466
PsuI RGATCY 3 cut(s) 226, 240, 2377
PvuII CAGCTG 1 cut(s) 2192
RsaI GTAC 3 cut(s) 1129, 1286, 2074
RsaNI GTAC 3 cut(s) 1128, 1285, 2073
RseI CAYNNNNRTG 5 cut(s) 249, 681, 976, 1119, 2328
SacI GAGCTC 1 cut(s) 1383
SapI GCTCTTC 1 cut(s) 1371
SaqAI TTAA 9 cut(s) 92, 599, 659, 798, 1415, 1911, 1983, 2219, 2445
SatI GCNGC 9 cut(s) 430, 747, 1047, 1389, 1467, 1515, 1759, 2190, 2466
Sau96I GGNCC 1 cut(s) 1133
SchI GAGTC 1 cut(s) 639
ScrFI CCNGG 5 cut(s) 46, 175, 1673, 1952, 2408
SduI GDGCHC 2 cut(s) 198, 1383
SfaNI GCATC 4 cut(s) 820, 1768, 1858, 2029
SfcI CTRYAG 7 cut(s) 430, 612, 1137, 1458, 1756, 2068, 2466
SmiMI CAYNNNNRTG 5 cut(s) 249, 681, 976, 1119, 2328
SmlI CTYRAG 5 cut(s) 598, 797, 1059, 1185, 1833
SmoI CTYRAG 5 cut(s) 598, 797, 1059, 1185, 1833
SphI GCATGC 1 cut(s) 883
SsiI CCGC 1 cut(s) 1251
SspMI CTAG 9 cut(s) 56, 62, 186, 594, 866, 1221, 1436, 1637, 2003
SstI GAGCTC 1 cut(s) 1383
StyD4I CCNGG 5 cut(s) 44, 173, 1671, 1950, 2406
StyI CCWWGG 2 cut(s) 816, 2154
TaaI ACNGT 5 cut(s) 275, 626, 1118, 1372, 2072
TaqI TCGA 3 cut(s) 78, 628, 2052
TatI WGTACW 1 cut(s) 1284
TfiI GAWTC 6 cut(s) 299, 491, 701, 1073, 1713, 2246
Tru1I TTAA 9 cut(s) 92, 599, 659, 798, 1415, 1911, 1983, 2219, 2445
Tru9I TTAA 9 cut(s) 92, 599, 659, 798, 1415, 1911, 1983, 2219, 2445
TscAI CASTG 6 cut(s) 513, 907, 940, 1086, 1123, 1155
TseFI GTSAC 5 cut(s) 392, 898, 1035, 1342, 2269
TseI GCWGC 9 cut(s) 429, 746, 1046, 1388, 1466, 1514, 1758, 2189, 2465
Tsp45I GTSAC 5 cut(s) 392, 898, 1035, 1342, 2269
TspRI CASTG 6 cut(s) 513, 907, 940, 1086, 1123, 1155
Van91I CCANNNNNTGG 1 cut(s) 1480
Vha464I CTTAAG 2 cut(s) 598, 797
VspI ATTAAT 1 cut(s) 2445
XapI RAATTY 4 cut(s) 352, 993, 1741, 2275
XbaI TCTAGA 2 cut(s) 185, 865
XceI RCATGY 4 cut(s) 202, 553, 883, 1210
XcmI CCANNNNNNNNNTGG 3 cut(s) 171, 566, 1477
XmnI GAANNNNTTC 1 cut(s) 1744
XspI CTAG 9 cut(s) 56, 62, 186, 594, 866, 1221, 1436, 1637, 2003
Zsp2I ATGCAT 1 cut(s) 2262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.