MD17G1259000.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007066
Physical Location & Seq
Reverse (-)
31928206 .. 31931139
2934 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1259000.v1.1.491

Sequence Viewer

Length: 2724 bp
ATGGCAGGCAACAAGTTTGCAACCATGTTGCACAGGAACACCCCGAAGTTGATTGTAATTCTAGTCTATGCTGTCCTTGAATGGATTTTGATAGCACTCCTCCTCCTCAACTCTTTGCTCAGTTACTTGATCACCAAATTTGCCGATTACTTTGGTCTCAAACAGCCTTGCCCGTTGTGTACTTATAGAATCAATCGCGTCTTGGAACAAGGCAACAGCTCAAACAACTCTTACACAGATGTTGTATGTGAGAGCCATGCCACGAAGATATCCAACATGTTTTCCCTTCCTTGTTCTCCACCGTCTGAGCCTTCTTGGGGCTCCTTGGAGTATGCCCGGAAAGGAGATTCAGCTGTAGAGGCTGCGGATGATGGAAACAATGGAGGCGAGTATCAAAGTGCAAACAAACCCGATAGCCTGTCAACTCACAGTGACAATGGCTATGAGTTGGAGACAACGAATGAAGAACATGAGGACGATAGAGTGGCAGACGAGCAACAACTTACTTCGGATGTTTGCAGTTCTAGCTTCAGAGGTGGTGCAGTGGAAGATTGTCTGAGGTCTAGGTCAGTATTGCTATGTCAGAAAAATGATGCAGATGAAGACAACAAAGGAGGTTCTCTTGAAATTGTGCGGTCAGATTCTAACAGCTCGAACCTTGTCCATCAATCCTCCGATGCTAGTGCTACTAGCATTCAGTGTTGCTTTGAAGAAGACTACTCTCTCGAAATCATCCCCCTATCTTCCGAGAATGGTTTGATCGGTGCTCACAATCACCGGTTGATTCCTGTGGAGTTGATTGATTTTTCAACTACAGTAGACCAGGGAATCCGCAATGTAAAAGAGGAAGAGGTGAGAGAGCATGATCATCATGAAGTGAATTTCGCTTCCGACTCATACATCGGATCTCGACCTCAACTCTCTGGAGAAGCATCATTAGTACTCATGGTAAACGAGAGTGCAGTTAACACAAGTAATGGAGAAGTTGAAAGCTTAGATATGGCGAGGGGTTTTACTGATAACTCTTCTGAGGTAGACGCGGAAGAGGGAAAACAAGATTTGCTTGGCAAGCCATGTGATGAAGTTGTCACTGATCCAGGAGCTCAAACATTATTTGTTCGAATTGAAGAACCTGATAACAAAGAACAAAATGATCCACCAGCATCTGAAGAAGAAAGTATTTCTGTTGATCACATTATACCAAGAAATTTGGCAAGCACAGAAGTGGGTGACCGTGAAACTGATCACCCTCGGGCTCAAGAAGGTTCTTCTTCGACGTGCTTTGAAATTGCCAATGTACCTGATACATTCTCGGATCAGAGTGAATACGGTTTAAGGCACACTGAAGAAGGTATACCGAAAGAGGAAACAAGTAAGCTCGAAAATGATCAAGAATCGGAGGAAGAGAAGATTCCGAACTCACCAACATCAGTAAACAGTCTTTGTTCCTTGCACAAGAAACCGGGTGAAAAAAAAGAGCCGGCGGTAGAAGAGTATTTGGATGGAAGTGGGGTCAGTGAGATGGAAGATGGAGATCCTTTTACAACGATTGGACGGCTCAAAGAAGCTTTGACAGCTGAGCGTAAAGCTATAAGTTCCTTGTACGCAGAACTAGAGCAAGAGAGGAGTGCGTCTGCAATTGCCGCCAACCAGACTATGGCTATGATAACAAGGCTTCAAGAAGAGAAGGCAGCAATGCAAATGGAGGCATTGCAATACCAGAGGATGATGGAAGAACAATCCGAGTACGATCAGGATGCCTTACAACTATTGAATGATTTGATGAACAAGAAGGAGAAGGAAAAGCAAGAACTCGAGAGAGAGCTGGAAATATATCAAAAGAAAGTCCTAGATTATGAGGAAAAAGAGAAGATAAGGATCATGAGCAGAATTAAATATGGAAGTGTAAAAAGTAGGAATTCTTCTGCTTCTTGCAGCCAGTCGTGGGATAGTGATGCGCTATCGATTGACCTTAATGTTGAATCCAGGGACGAAGAAAGTGGCTTCGGTGGCCTTCAAGAAAGCAACAACAACAACAATACTACAGATGATGCAGGTCTGAGTTTGGAAGATTTAGCTCTGGATTGTGTTAAAAATATGAGTGTTCTTGATGAATCGCTAGCAGGGTTCGAGGAAGAGAGGCTGTCCATTCTGGATCAGCTCAAAGCCTTGGAGGAGAAGCTAATCACGTTGGGTGCAAATGAGGAATTGGGCGATGACTTTAAATTAGTTGAGCATTCCCCCGCGTATAGTCTCAAAGACTTCGAAGAGAATCATGATTTTAGCAGTCCAGAGGAAAATGGGACGTCGAAGGGATTTTCGGAGGATAAACATCAACCCGAGGGAAAGACATTGGGTTCCATGGTAAAAAGACTGCTTCCGCTTTTGGATGCAACCGACAATGAAACCGAAGAAGGGTTAACTCATGAAGAACACATTGATCGATCAGAATCTATTGAAACAATGCGGAACTCAATTACCAATTTTGAGTTGAATGATGGCAAAATAGCCATTGAAGAGGAGGTGGATCATGTTTATGAGAGGCTACAAACTCTTGAAGCAGACAGGGAGTTTCTAAAGCACTGCATGAGCTCCATAAAGAAGGGAGACAAAGGGGTGGATCTTCTCCAAGAGATTTTGCAACATCTAAGGGATCTGAGGGCTGTTGAACTCCGCGTCAAGAACATGAATGATCCCGAGGGCGATGAAGTTGCAGTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

908

Amino Acids

101.08

Weight (kDa)

4.44

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 519 - 609 7.7e-33 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2309
Acc36I ACCTGC 1 cut(s) 2045
AccB7I CCANNNNNTGG 1 cut(s) 1657
AccI GTMKAC 3 cut(s) 819, 1035, 1354
AccII CGCG 4 cut(s) 198, 1040, 2246, 2676
AcsI RAATTY 4 cut(s) 137, 880, 1207, 1918
AcuI CTGAAG 3 cut(s) 514, 1188, 1365
AcyI GRCGYC 1 cut(s) 2306
AfaI GTAC 5 cut(s) 181, 942, 1299, 1604, 1748
AfiI CCNNNNNNNGG 4 cut(s) 317, 1657, 1945, 2415
AflIII ACRYGT 1 cut(s) 276
AgeI ACCGGT 1 cut(s) 777
AjiI CACGTC 1 cut(s) 1278
AjnI CCWGG 3 cut(s) 822, 1096, 1985
AleI CACNNNNGTG 1 cut(s) 1223
Alw21I GWGCWC 3 cut(s) 769, 1105, 2594
Alw26I GTCTC 4 cut(s) 161, 446, 2258, 2601
AlwNI CAGNNNCTG 1 cut(s) 1166
Ama87I CYCGRG 4 cut(s) 1251, 1814, 2339, 2696
AoxI GGCC 1 cut(s) 2011
ApeKI GCWGC 3 cut(s) 362, 1691, 1935
ApoI RAATTY 4 cut(s) 137, 880, 1207, 1918
AsiGI ACCGGT 1 cut(s) 777
AspLEI GCGC 1 cut(s) 1960
AsuC2I CCSGG 2 cut(s) 337, 1464
AsuHPI GGTGA 7 cut(s) 124, 767, 865, 1238, 1241, 1413, 1478
AsuII TTCGAA 2 cut(s) 1120, 2265
AsuNHI GCTAGC 1 cut(s) 2119
AvaI CYCGRG 4 cut(s) 1251, 1814, 2339, 2696
BanII GRGCYC 4 cut(s) 323, 1105, 1258, 2594
BarI GAAGNNNNNNTAC 4 cut(s) 1338, 1370, 1906, 1938
BbsI GAAGAC 2 cut(s) 609, 720
Bbv12I GWGCWC 3 cut(s) 769, 1105, 2594
BbvI GCAGC 3 cut(s) 349, 1703, 1947
BccI CCATC 7 cut(s) 365, 672, 1496, 1516, 1523, 1723, 2492
BceAI ACGGC 1 cut(s) 1571
BcgI CGANNNNNNTGC 5 cut(s) 665, 699, 1739, 1773, 2693
BciT130I CCWGG 3 cut(s) 824, 1098, 1987
BclI TGATCA 5 cut(s) 129, 865, 1189, 1243, 1387
BcnI CCSGG 2 cut(s) 337, 1464
BcoDI GTCTC 4 cut(s) 161, 446, 2258, 2601
BfaI CTAG 8 cut(s) 62, 525, 564, 681, 690, 1613, 1850, 2120
BfmI CTRYAG 3 cut(s) 354, 813, 2043
BfuAI ACCTGC 1 cut(s) 2045
BisI GCNGC 4 cut(s) 363, 1644, 1692, 1936
BlpI GCTNAGC 1 cut(s) 1578
BlsI GCNGC 4 cut(s) 364, 1645, 1693, 1937
BmcAI AGTACT 1 cut(s) 942
Bme1390I CCNGG 5 cut(s) 337, 824, 1098, 1464, 1987
BmeT110I CYCGRG 4 cut(s) 1251, 1814, 2339, 2696
BmgBI CACGTC 1 cut(s) 1278
BmiI GGNNCC 2 cut(s) 322, 2359
BmrFI CCNGG 5 cut(s) 337, 824, 1098, 1464, 1987
BmsI GCATC 8 cut(s) 583, 667, 941, 1172, 1747, 1945, 2041, 2380
BmtI GCTAGC 1 cut(s) 2123
BpiI GAAGAC 2 cut(s) 609, 720
BpmI CTGGAG 1 cut(s) 945
Bpu1102I GCTNAGC 1 cut(s) 1578
Bpu14I TTCGAA 2 cut(s) 1120, 2265
BpuEI CTTGAG 1 cut(s) 1242
BpuMI CCSGG 2 cut(s) 337, 1464
Bsa29I ATCGAT 2 cut(s) 1964, 2443
BsaBI GATNNNNATC 4 cut(s) 1533, 1877, 2331, 2449
BsaHI GRCGYC 1 cut(s) 2306
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 8 cut(s) 324, 823, 1250, 1986, 2169, 2340, 2361, 2697
BsaWI WCCGGW 1 cut(s) 777
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 4 cut(s) 317, 1657, 1945, 2415
Bse118I RCCGGY 2 cut(s) 777, 1480
Bse1I ACTGG 1 cut(s) 1939
Bse3DI GCAATG 3 cut(s) 841, 1701, 1709
Bse8I GATNNNNATC 4 cut(s) 1533, 1877, 2331, 2449
BseBI CCWGG 3 cut(s) 824, 1098, 1987
BseCI ATCGAT 2 cut(s) 1964, 2443
BseDI CCNNGG 8 cut(s) 324, 823, 1250, 1986, 2169, 2340, 2361, 2697
BseGI GGATG 7 cut(s) 373, 517, 732, 1507, 1731, 1762, 2395
BseJI GATNNNNATC 4 cut(s) 1533, 1877, 2331, 2449
BseLI CCNNNNNNNGG 4 cut(s) 317, 1657, 1945, 2415
BseMI GCAATG 3 cut(s) 841, 1701, 1709
BseMII CTCAG 7 cut(s) 133, 297, 548, 1020, 1569, 2051, 2648
BseNI ACTGG 1 cut(s) 1939
BseRI GAGGAG 6 cut(s) 89, 92, 95, 1639, 2189, 2534
BseXI GCAGC 3 cut(s) 349, 1703, 1947
BsgI GTGCAG 2 cut(s) 561, 981
Bsh1236I CGCG 4 cut(s) 198, 1040, 2246, 2676
BshFI GGCC 1 cut(s) 2013
BshTI ACCGGT 1 cut(s) 777
BshVI ATCGAT 2 cut(s) 1964, 2443
BsiHKAI GWGCWC 3 cut(s) 769, 1105, 2594
BsiHKCI CYCGRG 4 cut(s) 1251, 1814, 2339, 2696
BsiSI CCGG 4 cut(s) 337, 778, 1463, 1481
BslFI GGGAC 2 cut(s) 2003, 2317
BslI CCNNNNNNNGG 4 cut(s) 317, 1657, 1945, 2415
BsmAI GTCTC 4 cut(s) 161, 446, 2258, 2601
BsmFI GGGAC 2 cut(s) 2003, 2317
BsmI GAATGC 2 cut(s) 693, 2236
BsnI GGCC 1 cut(s) 2013
Bso31I GGTCTC 1 cut(s) 161
BsoBI CYCGRG 4 cut(s) 1251, 1814, 2339, 2696
Bsp119I TTCGAA 2 cut(s) 1120, 2265
Bsp1286I GDGCHC 5 cut(s) 323, 769, 1105, 1258, 2594
Bsp1720I GCTNAGC 1 cut(s) 1578
Bsp19I CCATGG 1 cut(s) 2361
BspANI GGCC 1 cut(s) 2013
BspCNI CTCAG 7 cut(s) 132, 298, 549, 1021, 1570, 2052, 2649
BspDI ATCGAT 2 cut(s) 1964, 2443
BspFNI CGCG 4 cut(s) 198, 1040, 2246, 2676
BspHI TCATGA 4 cut(s) 871, 1881, 2275, 2425
BspLI GGNNCC 2 cut(s) 322, 2359
BspMI ACCTGC 1 cut(s) 2045
BspOI GCTAGC 1 cut(s) 2123
BspT104I TTCGAA 2 cut(s) 1120, 2265
BspTNI GGTCTC 1 cut(s) 161
BsrDI GCAATG 3 cut(s) 841, 1701, 1709
BsrFI RCCGGY 2 cut(s) 777, 1480
BsrI ACTGG 1 cut(s) 1939
BssAI RCCGGY 2 cut(s) 777, 1480
BssECI CCNNGG 8 cut(s) 324, 823, 1250, 1986, 2169, 2340, 2361, 2697
BssNAI GTATAC 1 cut(s) 1355
BssNI GRCGYC 1 cut(s) 2306
BssT1I CCWWGG 3 cut(s) 324, 2169, 2361
Bst1107I GTATAC 1 cut(s) 1355
Bst2UI CCWGG 3 cut(s) 824, 1098, 1987
Bst4CI ACNGT 6 cut(s) 303, 431, 817, 1235, 1331, 1439
Bst6I CTCTTC 9 cut(s) 843, 1030, 1038, 1398, 1485, 1677, 2130, 2262, 2511
BstACI GRCGYC 1 cut(s) 2306
BstBI TTCGAA 2 cut(s) 1120, 2265
BstC8I GCNNGC 5 cut(s) 7, 1070, 1216, 1482, 2121
BstDEI CTNAG 9 cut(s) 119, 306, 557, 994, 1029, 1578, 2060, 2648, 2657
BstDSI CCRYGG 1 cut(s) 2361
BstEII GGTNACC 1 cut(s) 1229
BstF5I GGATG 7 cut(s) 373, 517, 732, 1507, 1731, 1762, 2395
BstFNI CGCG 4 cut(s) 198, 1040, 2246, 2676
BstHHI GCGC 1 cut(s) 1960
BstMAI GTCTC 4 cut(s) 161, 446, 2258, 2601
BstMWI GCNNNNNNNGC 6 cut(s) 359, 525, 1069, 1574, 1643, 2010
BstNI CCWGG 3 cut(s) 824, 1098, 1987
BstNSI RCATGY 1 cut(s) 280
BstPI GGTNACC 1 cut(s) 1229
BstSCI CCNGG 5 cut(s) 335, 822, 1096, 1462, 1985
BstSFI CTRYAG 3 cut(s) 354, 813, 2043
BstUI CGCG 4 cut(s) 198, 1040, 2246, 2676
BstV1I GCAGC 3 cut(s) 349, 1703, 1947
BstV2I GAAGAC 2 cut(s) 609, 720
BstX2I RGATCY 4 cut(s) 905, 1534, 2620, 2653
BstYI RGATCY 4 cut(s) 905, 1534, 2620, 2653
BstZ17I GTATAC 1 cut(s) 1355
Bsu15I ATCGAT 2 cut(s) 1964, 2443
BsuRI GGCC 1 cut(s) 2013
BsuTUI ATCGAT 2 cut(s) 1964, 2443
BtgI CCRYGG 1 cut(s) 2361
BtgZI GCGATG 2 cut(s) 2229, 2718
BtrI CACGTC 1 cut(s) 1278
BtsCI GGATG 7 cut(s) 373, 517, 732, 1507, 1731, 1762, 2395
BtsI GCAGTG 2 cut(s) 549, 2581
BtsIMutI CAGTG 7 cut(s) 436, 549, 704, 1089, 1341, 1522, 2581
BveI ACCTGC 1 cut(s) 2045
Cac8I GCNNGC 5 cut(s) 7, 1070, 1216, 1482, 2121
CaiI CAGNNNCTG 1 cut(s) 1166
CciI TCATGA 4 cut(s) 871, 1881, 2275, 2425
CfoI GCGC 1 cut(s) 1960
Cfr10I RCCGGY 2 cut(s) 777, 1480
ClaI ATCGAT 2 cut(s) 1964, 2443
CseI GACGC 4 cut(s) 187, 1046, 1620, 2665
Csp6I GTAC 5 cut(s) 180, 941, 1298, 1603, 1747
CspAI ACCGGT 1 cut(s) 777
CviQI GTAC 5 cut(s) 180, 941, 1298, 1603, 1747
DdeI CTNAG 9 cut(s) 119, 306, 557, 994, 1029, 1578, 2060, 2648, 2657
DraI TTTAAA 1 cut(s) 2224
Eam1104I CTCTTC 9 cut(s) 843, 1030, 1038, 1398, 1485, 1677, 2130, 2262, 2511
EarI CTCTTC 9 cut(s) 843, 1030, 1038, 1398, 1485, 1677, 2130, 2262, 2511
Ecl136II GAGCTC 2 cut(s) 1103, 2592
Eco130I CCWWGG 3 cut(s) 324, 2169, 2361
Eco24I GRGCYC 4 cut(s) 323, 1105, 1258, 2594
Eco31I GGTCTC 1 cut(s) 161
Eco32I GATATC 1 cut(s) 270
Eco53kI GAGCTC 2 cut(s) 1103, 2592
Eco57I CTGAAG 3 cut(s) 514, 1188, 1365
Eco88I CYCGRG 4 cut(s) 1251, 1814, 2339, 2696
Eco91I GGTNACC 1 cut(s) 1229
EcoICRI GAGCTC 2 cut(s) 1103, 2592
EcoO65I GGTNACC 1 cut(s) 1229
EcoRI GAATTC 1 cut(s) 1918
EcoRII CCWGG 3 cut(s) 822, 1096, 1985
EcoRV GATATC 1 cut(s) 270
EcoT14I CCWWGG 3 cut(s) 324, 2169, 2361
EcoT38I GRGCYC 4 cut(s) 323, 1105, 1258, 2594
ErhI CCWWGG 3 cut(s) 324, 2169, 2361
FalI AAGNNNNNCTT 2 cut(s) 1047, 1079
FaqI GGGAC 2 cut(s) 2003, 2317
FauI CCCGC 1 cut(s) 2251
FbaI TGATCA 5 cut(s) 129, 865, 1189, 1243, 1387
FblI GTMKAC 3 cut(s) 819, 1035, 1354
Fnu4HI GCNGC 4 cut(s) 363, 1644, 1692, 1936
FokI GGATG 7 cut(s) 380, 524, 719, 1514, 1738, 1769, 2402
FriOI GRGCYC 4 cut(s) 323, 1105, 1258, 2594
Fsp4HI GCNGC 4 cut(s) 363, 1644, 1692, 1936
FspBI CTAG 8 cut(s) 62, 525, 564, 681, 690, 1613, 1850, 2120
GlaI GCGC 1 cut(s) 1959
GluI GCNGC 4 cut(s) 363, 1644, 1692, 1936
GsuI CTGGAG 1 cut(s) 945
HaeIII GGCC 1 cut(s) 2013
HapII CCGG 4 cut(s) 337, 778, 1463, 1481
HgaI GACGC 4 cut(s) 187, 1046, 1620, 2665
HhaI GCGC 1 cut(s) 1960
Hin1I GRCGYC 1 cut(s) 2306
Hin6I GCGC 1 cut(s) 1958
HinP1I GCGC 1 cut(s) 1958
HincII GTYRAC 3 cut(s) 423, 967, 2421
HindII GTYRAC 3 cut(s) 423, 967, 2421
HindIII AAGCTT 2 cut(s) 991, 1566
HpaI GTTAAC 2 cut(s) 967, 2421
HpaII CCGG 4 cut(s) 337, 778, 1463, 1481
HphI GGTGA 7 cut(s) 124, 767, 865, 1238, 1241, 1413, 1478
Hpy166II GTNNAC 9 cut(s) 180, 423, 820, 952, 967, 1036, 1355, 1435, 2421
Hpy8I GTNNAC 9 cut(s) 180, 423, 820, 952, 967, 1036, 1355, 1435, 2421
Hpy99I CGWCG 2 cut(s) 1279, 2311
HpyCH4III ACNGT 6 cut(s) 303, 431, 817, 1235, 1331, 1439
HpyCH4IV ACGT 3 cut(s) 1277, 2189, 2306
HpyF10VI GCNNNNNNNGC 6 cut(s) 359, 525, 1069, 1574, 1643, 2010
HpyF3I CTNAG 9 cut(s) 119, 306, 557, 994, 1029, 1578, 2060, 2648, 2657
HpySE526I ACGT 3 cut(s) 1277, 2189, 2306
Hsp92I GRCGYC 1 cut(s) 2306
HspAI GCGC 1 cut(s) 1958
KroI GCCGGC 1 cut(s) 1480
KroNI GCCGGC 1 cut(s) 1482
Ksp22I TGATCA 5 cut(s) 129, 865, 1189, 1243, 1387
KspAI GTTAAC 2 cut(s) 967, 2421
LmnI GCTCC 3 cut(s) 326, 1100, 2597
Lsp1109I GCAGC 3 cut(s) 349, 1703, 1947
LweI GCATC 8 cut(s) 583, 667, 941, 1172, 1747, 1945, 2041, 2380
MaeI CTAG 8 cut(s) 62, 525, 564, 681, 690, 1613, 1850, 2120
MaeII ACGT 3 cut(s) 1277, 2189, 2306
MaeIII GTNAC 4 cut(s) 122, 431, 1087, 1229
MfeI CAATTG 1 cut(s) 1638
MflI RGATCY 4 cut(s) 905, 1534, 2620, 2653
MhlI GDGCHC 5 cut(s) 323, 769, 1105, 1258, 2594
MlyI GAGTC 1 cut(s) 887
MmeI TCCRAC 3 cut(s) 297, 429, 915
MroNI GCCGGC 1 cut(s) 1480
MseI TTAA 7 cut(s) 966, 1334, 1893, 1974, 2091, 2223, 2420
MslI CAYNNNNRTG 2 cut(s) 1223, 2535
MspA1I CMGCKG 2 cut(s) 353, 1577
MspI CCGG 4 cut(s) 337, 778, 1463, 1481
MspR9I CCNGG 5 cut(s) 337, 824, 1098, 1464, 1987
MunI CAATTG 1 cut(s) 1638
Mva1269I GAATGC 2 cut(s) 693, 2236
MvaI CCWGG 3 cut(s) 824, 1098, 1987
MvnI CGCG 4 cut(s) 198, 1040, 2246, 2676
MwoI GCNNNNNNNGC 6 cut(s) 359, 525, 1069, 1574, 1643, 2010
NaeI GCCGGC 1 cut(s) 1482
NciI CCSGG 2 cut(s) 337, 1464
NcoI CCATGG 1 cut(s) 2361
NgoMIV GCCGGC 1 cut(s) 1480
NheI GCTAGC 1 cut(s) 2119
NlaIV GGNNCC 2 cut(s) 322, 2359
NmuCI GTSAC 3 cut(s) 431, 1087, 1229
NspI RCATGY 1 cut(s) 280
NspV TTCGAA 2 cut(s) 1120, 2265
OliI CACNNNNGTG 1 cut(s) 1223
PaeR7I CTCGAG 1 cut(s) 1814
PagI TCATGA 4 cut(s) 871, 1881, 2275, 2425
PciI ACATGT 1 cut(s) 276
PctI GAATGC 2 cut(s) 693, 2236
PdiI GCCGGC 1 cut(s) 1482
PflMI CCANNNNNTGG 1 cut(s) 1657
PfoI TCCNGGA 1 cut(s) 1096
PinAI ACCGGT 1 cut(s) 777
PkrI GCNGC 4 cut(s) 364, 1645, 1693, 1937
PleI GAGTC 1 cut(s) 887
PpsI GAGTC 1 cut(s) 887
PscI ACATGT 1 cut(s) 276
Psp124BI GAGCTC 2 cut(s) 1105, 2594
Psp6I CCWGG 3 cut(s) 822, 1096, 1985
PspEI GGTNACC 1 cut(s) 1229
PspGI CCWGG 3 cut(s) 822, 1096, 1985
PspN4I GGNNCC 2 cut(s) 322, 2359
PstNI CAGNNNCTG 1 cut(s) 1166
PsuI RGATCY 4 cut(s) 905, 1534, 2620, 2653
PvuII CAGCTG 2 cut(s) 353, 1577
RsaI GTAC 5 cut(s) 181, 942, 1299, 1604, 1748
RsaNI GTAC 5 cut(s) 180, 941, 1298, 1603, 1747
RseI CAYNNNNRTG 2 cut(s) 1223, 2535
SacI GAGCTC 2 cut(s) 1105, 2594
SaqAI TTAA 7 cut(s) 966, 1334, 1893, 1974, 2091, 2223, 2420
SatI GCNGC 4 cut(s) 363, 1644, 1692, 1936
ScaI AGTACT 1 cut(s) 942
SchI GAGTC 1 cut(s) 887
ScrFI CCNGG 5 cut(s) 337, 824, 1098, 1464, 1987
SduI GDGCHC 5 cut(s) 323, 769, 1105, 1258, 2594
SfaNI GCATC 8 cut(s) 583, 667, 941, 1172, 1747, 1945, 2041, 2380
SfcI CTRYAG 3 cut(s) 354, 813, 2043
Sfr274I CTCGAG 1 cut(s) 1814
SfuI TTCGAA 2 cut(s) 1120, 2265
SlaI CTCGAG 1 cut(s) 1814
SmiMI CAYNNNNRTG 2 cut(s) 1223, 2535
SmlI CTYRAG 2 cut(s) 1257, 1814
SmoI CTYRAG 2 cut(s) 1257, 1814
SspMI CTAG 8 cut(s) 62, 525, 564, 681, 690, 1613, 1850, 2120
SstI GAGCTC 2 cut(s) 1105, 2594
StyD4I CCNGG 5 cut(s) 335, 822, 1096, 1462, 1985
StyI CCWWGG 3 cut(s) 324, 2169, 2361
TaaI ACNGT 6 cut(s) 303, 431, 817, 1235, 1331, 1439
TaiI ACGT 3 cut(s) 1280, 2192, 2309
TatI WGTACW 2 cut(s) 179, 940
TauI GCSGC 1 cut(s) 1646
Tru1I TTAA 7 cut(s) 966, 1334, 1893, 1974, 2091, 2223, 2420
Tru9I TTAA 7 cut(s) 966, 1334, 1893, 1974, 2091, 2223, 2420
TscAI CASTG 7 cut(s) 436, 549, 704, 1096, 1348, 1522, 2588
TseFI GTSAC 3 cut(s) 431, 1087, 1229
TseI GCWGC 3 cut(s) 362, 1691, 1935
Tsp45I GTSAC 3 cut(s) 431, 1087, 1229
TspRI CASTG 7 cut(s) 436, 549, 704, 1096, 1348, 1522, 2588
Van91I CCANNNNNTGG 1 cut(s) 1657
XapI RAATTY 4 cut(s) 137, 880, 1207, 1918
XceI RCATGY 1 cut(s) 280
XcmI CCANNNNNNNNNTGG 1 cut(s) 1654
XhoI CTCGAG 1 cut(s) 1814
XmiI GTMKAC 3 cut(s) 819, 1035, 1354
XspI CTAG 8 cut(s) 62, 525, 564, 681, 690, 1613, 1850, 2120
ZraI GACGTC 1 cut(s) 2307
ZrmI AGTACT 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.