RLG00000022726

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0007066
Physical Location & Seq
Reverse (-)
10659251 .. 10666578
7328 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022726

Sequence Viewer

Length: 3489 bp
ATGTTGAAGAGGAAAAGTCCCAGGTTCGTTCTAGTTCTAGTCTATGCAGTCCTCGAATGGATGTTGTTAATACTCCTCCTGCTCAACTCTTTTTTCAGTTACTTGATCACAAAGTTTGCCAACTATTTTGGCCTCAAACCACCTTGTCGCTGGTGTTCTTCTAGAGTTGAACACATGTTGGAACCACAAAAGTCTTACTCAGATCTTGTATGTGAGATCCATGCCAATGAGATATCTAAACTGGGTTACTGTCTCAATCATAAAAGACTGGCTGAATCACATAGTATGTGTGGGAACTGCTTTTCTTCTCAACCAAATTGCAGAGAAAATTCTACTCAAAGTAGGACGGATTTGATTTCATTGCTGAGTGACAAATATCAGAAGAAACCTTCCATCTGTTCTTGCTGCAGTAAGAGCTTCAACACCAAACTCTCTCCTCTTTCATTGGAGTATGCTCAGAAAGGAGATTCAAATATAGAAGCAGTGCATGATGACAACAATGAAGGCACAACCAAACCTGATAGCATGTCAGTCCACCGCGAAAATGGCTATGAGATGGAGACAACTTCCAGCTTAAGAGACACTGCTGCAGAAGAACACTGTCGAAATGAAAGTAAGAAACTTGTCCATCAATCTTCCAATGCTACTACCATTGAGTACTGTTGCCTTCAAGAAGATCACTCTCTTGAAACTACCCCCCTTCCTTCTAAGAGTGGTATGGTCTGTGATCTTGGTTGCAGCCTGATTCCTACTGATTTGATTGATTTTTCAACGACAATAGACAAAGGACTCATAAACAGCTTAAAACATCAGGAACATCATGATCATGAGCAAGGAAGTTTTGATTCCAAGTCAAAGATTGAATTGGAAGATCAACTCTCACGGGAAGCAGCATTATTTATGGTAAATAAGAGTGGAGCAAATACAAGTAATGGAGGACTTAAGAGCTTGGAGATGGCCAAGGGATTTACTGATGCTTCATCTGTTGAACAAGTGGAAAAGCGAAATCTAGAGTTGGTTGGCAAGCTATGTGATGATTTGGTCACTGCTCCAGAAGCTCAAACTTCGTTCGTTGACACTGAACAACTAACAGATACCAAACAACCTAACGATCCACCAGAATGTGAAGAAGAGAGAATTTTGGCTGTCATTTCAAGAAATCTGTCAAACACAAAAAGGTCACACCATGCAGCCGATCAGCCTCAAGCTCACGAGGATTCTTCACTGCCATTCCTTGAAATATCCAATGTGCCAGCTCACAGTGATGTCGGTACAGGGCCTACAGGAAAACAAATGTTGCAAGACAAAACAAGTATAACTAAAAATGCTCAAGAAGTCAACCATTTTTGCATGTGTTCAGAGCCCAGTGAACATGAGGAAGAGAGGTTGGTATGCGGACAAAATTGTATAAACACTATCCATTACTTGTACAAAAAGCCGAATGAAAATGGAGAATGTGGGGCAGATGATGGAAGTGTGGCAAGTGTGACGAATGGTGGAGAACCTGTAACAACCATTGAAGAGCTCAAAGCAGCATTGAAGGCTGAAAGGAAAGCTCTAAGTGCTTTATATTCAGAACTAGAGGAAGAGAGAAGTGCGTCTGCAGTAGCTGCCAACCAGACTATGGCTATGATAACAAGGCTTCAAGAAGAGAAGGCAGCAATGCAGATGGAGGCATTGCAATATCAGAGAATGATGGAAGAACAATCTGAGTATGATCAGGAAGCCTTGCAACTTTTGAATGAGTTGATGAACAAATTGGAGAGGGAAAAACAAGAGCTAGAGAAAGAATTGGAAATATATCAGAAGAAAGTCCTGGATTATGAGGAAAAAGAGAAGATAAGGGTCATGAGCAGAATCAAATATGGAAGTATGAGAAGTAGAAATTCTTCTGCTTCCTGCAGCCAATCTTGGGATAGTGATGCGCTATCCATTGATCTCAATTGTGAAGCAAGGGATGAAGATGGTTCTTTCCCTCAAGAATGCAGTAGCAACATCAATAATCCAGATGATGCAGAAGCTCTAAGCTTGGAAGAAATGGCTCTGGATTGTGTTAAGCAGATGAGCATTCTTGATGACTCATTGGCAGGCTTCCAGGAAGAGAGGTTGTCAGTTCTGGATCAGCTTAAAGCATTGGAAGATAGGCTAGTAGTGTTGAGTGAAAATGAGAACTACTCTCAGGATGCTAATTCAGTCAAGAATTGCTCGAACTACAGTAACAAAAACATTGAAGAAAATCATGATTTCAGCAGTCCAGAAGAAAAACAAATATCAGATGAGAAAATAACATTGGCTTCCATGGCAAAGAGGCTTCTTCCGCTTTTGGGTGTAGCAGCTGAGGATAATGAAACTGAAGCAGGTTTAACAAATGAAAAACAAGTCAGAACTGAATCTATTGCAATGCATAACTCGGTGACCAATTCTGAATTGCATGATACCAGAATAGCCATTGAGGAGGAGGTGGATCATGTTTATGAGAGGCTACAAGCTCTTGAAAAAAGTAATTTGGAGAAGGGGATGGATCTTCTTCAAGAGAGCTTGCGACATCTCCGGGATTTGAGGGATGTTGAACTCCGCATAAGAAACATTAATGTTCCTGTGGATGAAGCTGCAGCCGAAGCTGTAACTAGCATGTCTTTCGGGCTCAACTGGTACAACAATGATCAATTGATGTCCAAGAATTTGCAAGAGCTCCTTGATGATTGCTGCATTTACCTAGCACAGGACAGTTGTATGGGAGCTAGGCTCTTCGATCTAATCTTGGCGCGTTTCTCTTATCTTTATCGCTTTCTAGAGAGTGAGAGAGACTGTGAAAGGAGAAGAGGCCCCAATCGATCTGGATTCGTTTGGGTAGAGGTGCAGAGACGGAGAGAGATTGTGGAAGGAGAAGAGCAGGAATCGGTGTTGGTTTGGTCGGGAAGAGGTGGGTGCGAATGTTTGGATCCAGTGTCAGCAAAAGCAAACTTCCAGGGAAGTTTAGCACAGGCGAGATTCCTGCCAAGAATTGGACAATTTATCGATGCTGAGAGTGATAGTTTTATAGTTGTCCTGCATTTAGAAACTCTAGAAGCTGAGATCAAGATGCTCAGGAAGAAGTTGACCTTTAAAGCAATTACAATGCCTAACGCTGATCCTCCTCCTAATATCTCTAGAAACAGCTCTTGGAGCGGTGGGAGGGGAAATGAACTTTTGGCAAATGAATCGGTGACGGCTATGGAGGGCCTGTCTTTGACTGTGGGGAAGGCCGTGGGTGCTTCTGTGATGGAGGTGGAGGCGGGCAAGGGAGGTACTGATCTGGTTTGTTTGGAGGCTGTCTCGGGGTCAAAGGGTCAGGGTGTTGGGACTTTACGGATTGGGAGGGAAGCTAGAGCTGGAGTGTCCAGTGGTGTCTTGAGTTCCAAGATGATGGGAAAACAGCTGGGGATGGAGCCGGTCCTGCAACAGTTGAAGCCTCATGATCCTTTGCTTATCACTCAGCATTGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008092 GO:0017022
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

1163

Amino Acids

129.88

Weight (kDa)

4.93

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 508 - 598 1.8e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2348
AccB7I CCANNNNNTGG 2 cut(s) 1624, 3005
AccBSI CCGCTC 1 cut(s) 3168
AccII CGCG 2 cut(s) 540, 2767
AciI CCGC 6 cut(s) 538, 1395, 2318, 2575, 3168, 3275
AclWI GGATC 9 cut(s) 211, 1106, 2127, 2472, 2529, 2936, 2949, 3125, 3452
AcoI YGGCCR 1 cut(s) 957
AcsI RAATTY 4 cut(s) 328, 1137, 1885, 2680
AcuI CTGAAG 1 cut(s) 2373
AfaI GTAC 5 cut(s) 659, 1273, 1430, 2654, 3289
AfiI CCNNNNNNNGG 5 cut(s) 1624, 1912, 2324, 2722, 3005
AflII CTTAAG 2 cut(s) 574, 941
AflIII ACRYGT 1 cut(s) 174
AjnI CCWGG 4 cut(s) 20, 1815, 2094, 2967
AjuI GAANNNNNNNTTGG 4 cut(s) 2273, 2305, 3145, 3177
Alw21I GWGCWC 2 cut(s) 1527, 2694
Alw26I GTCTC 6 cut(s) 257, 554, 573, 2799, 2857, 3319
AlwI GGATC 9 cut(s) 211, 1106, 2127, 2472, 2529, 2936, 2949, 3125, 3452
AlwNI CAGNNNCTG 1 cut(s) 1610
Ama87I CYCGRG 1 cut(s) 3316
AoxI GGCC 6 cut(s) 130, 957, 1277, 2824, 3220, 3243
ApoI RAATTY 4 cut(s) 328, 1137, 1885, 2680
AseI ATTAAT 1 cut(s) 2589
Asp700I GAANNNNTTC 1 cut(s) 1888
AspLEI GCGC 2 cut(s) 1927, 2767
AspS9I GGNCC 4 cut(s) 1277, 2825, 3220, 3433
AsuC2I CCSGG 1 cut(s) 2552
AsuHPI GGTGA 2 cut(s) 2425, 3217
AvaI CYCGRG 1 cut(s) 3316
AvaII GGWCC 1 cut(s) 3433
BalI TGGCCA 1 cut(s) 959
BamHI GGATCC 1 cut(s) 2941
BanII GRGCYC 4 cut(s) 1365, 1527, 2646, 2694
BarI GAAGNNNNNNTAC 2 cut(s) 1873, 1905
BauI CACGAG 1 cut(s) 1211
Bbv12I GWGCWC 2 cut(s) 1527, 2694
BbvCI CCTCAGC 1 cut(s) 2337
BceAI ACGGC 2 cut(s) 3225, 3230
BcgI CGANNNNNNTGC 6 cut(s) 2620, 2654, 2977, 3011, 3183, 3217
BciT130I CCWGG 4 cut(s) 22, 1817, 2096, 2969
BclI TGATCA 4 cut(s) 105, 823, 1717, 2662
BcnI CCSGG 1 cut(s) 2552
BcoDI GTCTC 6 cut(s) 257, 554, 573, 2799, 2857, 3319
BfmI CTRYAG 7 cut(s) 406, 588, 1281, 1602, 1900, 2212, 2610
BfrI CTTAAG 2 cut(s) 574, 941
BfuAI ACCTGC 1 cut(s) 2348
BglII AGATCT 1 cut(s) 202
BmcAI AGTACT 1 cut(s) 659
Bme1390I CCNGG 5 cut(s) 22, 1817, 2096, 2552, 2969
Bme18I GGWCC 1 cut(s) 3433
BmeT110I CYCGRG 1 cut(s) 3316
BmgT120I GGNCC 4 cut(s) 1277, 2825, 3220, 3433
BmiI GGNNCC 4 cut(s) 183, 2827, 2943, 3429
BmrFI CCNGG 5 cut(s) 22, 1817, 2096, 2552, 2969
BmrI ACTGGG 2 cut(s) 251, 1359
BmsI GCATC 6 cut(s) 964, 1912, 2002, 2173, 3010, 3072
BmuI ACTGGG 2 cut(s) 251, 1359
BplI GAGNNNNNCTC 6 cut(s) 2159, 2191, 2730, 2762, 3299, 3331
BpmI CTGGAG 2 cut(s) 1035, 3393
Bpu10I CCTNAGC 2 cut(s) 2337, 3086
BpuEI CTTGAG 4 cut(s) 1188, 1314, 1962, 3412
BpuMI CCSGG 1 cut(s) 2552
Bsa29I ATCGAT 2 cut(s) 2833, 3018
BsaJI CCNNGG 5 cut(s) 20, 960, 2298, 2968, 3246
BsaXI ACNNNNNCTCC 4 cut(s) 1491, 1521, 3209, 3239
Bsc4I CCNNNNNNNGG 5 cut(s) 1624, 1912, 2324, 2722, 3005
Bse118I RCCGGY 1 cut(s) 3430
Bse1I ACTGG 6 cut(s) 246, 273, 1365, 2654, 2945, 3381
Bse3DI GCAATG 4 cut(s) 359, 1668, 1676, 2406
BseBI CCWGG 4 cut(s) 22, 1817, 2096, 2969
BseCI ATCGAT 2 cut(s) 2833, 3018
BseDI CCNNGG 5 cut(s) 20, 960, 2298, 2968, 3246
BseGI GGATG 7 cut(s) 66, 1963, 2188, 2523, 2569, 2608, 3429
BseLI CCNNNNNNNGG 5 cut(s) 1624, 1912, 2324, 2722, 3005
BseMI GCAATG 4 cut(s) 359, 1668, 1676, 2406
BseNI ACTGG 6 cut(s) 246, 273, 1365, 2654, 2945, 3381
BseRI GAGGAG 5 cut(s) 65, 426, 2468, 2471, 3126
BseYI CCCAGC 1 cut(s) 3418
BsgI GTGCAG 1 cut(s) 2879
Bsh1236I CGCG 2 cut(s) 540, 2767
BshFI GGCC 6 cut(s) 132, 959, 1279, 2826, 3222, 3245
BshVI ATCGAT 2 cut(s) 2833, 3018
BsiHKAI GWGCWC 2 cut(s) 1527, 2694
BsiHKCI CYCGRG 1 cut(s) 3316
BsiSI CCGG 2 cut(s) 2551, 3431
BslFI GGGAC 2 cut(s) 3, 3355
BslI CCNNNNNNNGG 5 cut(s) 1624, 1912, 2324, 2722, 3005
BsmAI GTCTC 6 cut(s) 257, 554, 573, 2799, 2857, 3319
BsmBI CGTCTC 1 cut(s) 2857
BsmFI GGGAC 2 cut(s) 3, 3355
BsmI GAATGC 2 cut(s) 1988, 2067
BsnI GGCC 6 cut(s) 132, 959, 1279, 2826, 3222, 3245
BsoBI CYCGRG 1 cut(s) 3316
Bsp1286I GDGCHC 4 cut(s) 1365, 1527, 2646, 2694
Bsp1407I TGTACA 1 cut(s) 1428
Bsp19I CCATGG 1 cut(s) 2298
BspACI CCGC 6 cut(s) 538, 1395, 2318, 2575, 3168, 3275
BspANI GGCC 6 cut(s) 132, 959, 1279, 2826, 3222, 3245
BspDI ATCGAT 2 cut(s) 2833, 3018
BspFNI CGCG 2 cut(s) 540, 2767
BspHI TCATGA 5 cut(s) 820, 826, 1848, 2239, 3454
BspLI GGNNCC 4 cut(s) 183, 2827, 2943, 3429
BspMAI CTGCAG 5 cut(s) 410, 592, 1606, 1904, 2614
BspMI ACCTGC 1 cut(s) 2348
BspPI GGATC 9 cut(s) 211, 1106, 2127, 2472, 2529, 2936, 2949, 3125, 3452
BspQI GCTCTTC 3 cut(s) 1515, 2753, 2883
BspTI CTTAAG 2 cut(s) 574, 941
BsrBI CCGCTC 1 cut(s) 3168
BsrDI GCAATG 4 cut(s) 359, 1668, 1676, 2406
BsrFI RCCGGY 1 cut(s) 3430
BsrGI TGTACA 1 cut(s) 1428
BsrI ACTGG 6 cut(s) 246, 273, 1365, 2654, 2945, 3381
BssAI RCCGGY 1 cut(s) 3430
BssECI CCNNGG 5 cut(s) 20, 960, 2298, 2968, 3246
BssSI CACGAG 1 cut(s) 1211
BssT1I CCWWGG 2 cut(s) 960, 2298
Bst2BI CACGAG 1 cut(s) 1211
Bst2UI CCWGG 4 cut(s) 22, 1817, 2096, 2969
Bst4CI ACNGT 9 cut(s) 251, 602, 662, 1262, 2216, 2729, 2810, 3235, 3444
BstAFI CTTAAG 2 cut(s) 574, 941
BstAPI GCANNNNNTGC 1 cut(s) 1610
BstAUI TGTACA 1 cut(s) 1428
BstC8I GCNNGC 5 cut(s) 1025, 1254, 2089, 2540, 3277
BstDSI CCRYGG 2 cut(s) 2298, 3246
BstEII GGTNACC 1 cut(s) 2413
BstENI CCTNNNNNAGG 1 cut(s) 2720
BstF5I GGATG 7 cut(s) 66, 1963, 2188, 2523, 2569, 2608, 3429
BstFNI CGCG 2 cut(s) 540, 2767
BstHHI GCGC 2 cut(s) 1927, 2767
BstMAI GTCTC 6 cut(s) 257, 554, 573, 2799, 2857, 3319
BstNI CCWGG 4 cut(s) 22, 1817, 2096, 2969
BstNSI RCATGY 4 cut(s) 178, 529, 1354, 2635
BstPI GGTNACC 1 cut(s) 2413
BstSCI CCNGG 5 cut(s) 20, 1815, 2094, 2550, 2967
BstSFI CTRYAG 7 cut(s) 406, 588, 1281, 1602, 1900, 2212, 2610
BstUI CGCG 2 cut(s) 540, 2767
BstX2I RGATCY 4 cut(s) 202, 216, 2521, 2941
BstXI CCANNNNNNTGG 1 cut(s) 3406
BstYI RGATCY 4 cut(s) 202, 216, 2521, 2941
Bsu15I ATCGAT 2 cut(s) 2833, 3018
BsuRI GGCC 6 cut(s) 132, 959, 1279, 2826, 3222, 3245
BsuTUI ATCGAT 2 cut(s) 2833, 3018
BtgI CCRYGG 2 cut(s) 2298, 3246
BtsCI GGATG 7 cut(s) 66, 1963, 2188, 2523, 2569, 2608, 3429
BtsI GCAGTG 4 cut(s) 489, 582, 1044, 1223
BveI ACCTGC 1 cut(s) 2348
Cac8I GCNNGC 5 cut(s) 1025, 1254, 2089, 2540, 3277
CaiI CAGNNNCTG 1 cut(s) 1610
CciI TCATGA 5 cut(s) 820, 826, 1848, 2239, 3454
CfoI GCGC 2 cut(s) 1927, 2767
Cfr10I RCCGGY 1 cut(s) 3430
Cfr13I GGNCC 4 cut(s) 1277, 2825, 3220, 3433
ClaI ATCGAT 2 cut(s) 2833, 3018
CseI GACGC 1 cut(s) 1587
Csp6I GTAC 5 cut(s) 658, 1272, 1429, 2653, 3288
CviQI GTAC 5 cut(s) 658, 1272, 1429, 2653, 3288
DraI TTTAAA 1 cut(s) 3106
EaeI YGGCCR 1 cut(s) 957
Ecl136II GAGCTC 2 cut(s) 1525, 2692
Eco130I CCWWGG 2 cut(s) 960, 2298
Eco24I GRGCYC 4 cut(s) 1365, 1527, 2646, 2694
Eco32I GATATC 1 cut(s) 234
Eco47I GGWCC 1 cut(s) 3433
Eco53kI GAGCTC 2 cut(s) 1525, 2692
Eco57I CTGAAG 1 cut(s) 2373
Eco88I CYCGRG 1 cut(s) 3316
Eco91I GGTNACC 1 cut(s) 2413
EcoICRI GAGCTC 2 cut(s) 1525, 2692
EcoNI CCTNNNNNAGG 1 cut(s) 2720
EcoO109I RGGNCCY 3 cut(s) 1277, 2825, 3220
EcoO65I GGTNACC 1 cut(s) 2413
EcoRII CCWGG 4 cut(s) 20, 1815, 2094, 2967
EcoRV GATATC 1 cut(s) 234
EcoT14I CCWWGG 2 cut(s) 960, 2298
EcoT22I ATGCAT 1 cut(s) 2406
EcoT38I GRGCYC 4 cut(s) 1365, 1527, 2646, 2694
ErhI CCWWGG 2 cut(s) 960, 2298
Esp3I CGTCTC 1 cut(s) 2857
FalI AAGNNNNNCTT 4 cut(s) 2679, 2711, 3086, 3118
FaqI GGGAC 2 cut(s) 3, 3355
FauI CCCGC 1 cut(s) 3268
FbaI TGATCA 4 cut(s) 105, 823, 1717, 2662
FokI GGATG 7 cut(s) 73, 1970, 2195, 2530, 2576, 2615, 3436
FriOI GRGCYC 4 cut(s) 1365, 1527, 2646, 2694
GlaI GCGC 2 cut(s) 1926, 2766
GsaI CCCAGC 1 cut(s) 3422
GsuI CTGGAG 2 cut(s) 1035, 3393
HaeIII GGCC 6 cut(s) 132, 959, 1279, 2826, 3222, 3245
HapII CCGG 2 cut(s) 2551, 3431
HgaI GACGC 1 cut(s) 1587
HhaI GCGC 2 cut(s) 1927, 2767
Hin6I GCGC 2 cut(s) 1925, 2765
HinP1I GCGC 2 cut(s) 1925, 2765
HincII GTYRAC 3 cut(s) 1075, 1339, 3099
HindII GTYRAC 3 cut(s) 1075, 1339, 3099
HindIII AAGCTT 1 cut(s) 2026
HpaII CCGG 2 cut(s) 2551, 3431
HphI GGTGA 2 cut(s) 2425, 3217
Hpy166II GTNNAC 5 cut(s) 535, 1075, 1339, 1370, 3099
Hpy8I GTNNAC 5 cut(s) 535, 1075, 1339, 1370, 3099
HpyCH4III ACNGT 9 cut(s) 251, 602, 662, 1262, 2216, 2729, 2810, 3235, 3444
HspAI GCGC 2 cut(s) 1925, 2765
Ksp22I TGATCA 4 cut(s) 105, 823, 1717, 2662
LguI GCTCTTC 3 cut(s) 1515, 2753, 2883
LmnI GCTCC 6 cut(s) 917, 1054, 2697, 2738, 3165, 3427
LweI GCATC 6 cut(s) 964, 1912, 2002, 2173, 3010, 3072
MbiI CCGCTC 1 cut(s) 3168
MfeI CAATTG 2 cut(s) 1942, 2666
MflI RGATCY 4 cut(s) 202, 216, 2521, 2941
MhlI GDGCHC 4 cut(s) 1365, 1527, 2646, 2694
MlsI TGGCCA 1 cut(s) 959
MluNI TGGCCA 1 cut(s) 959
MlyI GAGTC 2 cut(s) 783, 2072
MmeI TCCRAC 1 cut(s) 159
Mox20I TGGCCA 1 cut(s) 959
Mph1103I ATGCAT 1 cut(s) 2406
MroXI GAANNNNTTC 1 cut(s) 1888
MscI TGGCCA 1 cut(s) 959
MseI TTAA 9 cut(s) 68, 575, 803, 942, 2055, 2127, 2363, 2589, 3105
MslI CAYNNNNRTG 5 cut(s) 225, 825, 1120, 1263, 2472
Msp20I TGGCCA 1 cut(s) 959
MspA1I CMGCKG 2 cut(s) 2336, 3418
MspCI CTTAAG 2 cut(s) 574, 941
MspI CCGG 2 cut(s) 2551, 3431
MspR9I CCNGG 5 cut(s) 22, 1817, 2096, 2552, 2969
MunI CAATTG 2 cut(s) 1942, 2666
Mva1269I GAATGC 2 cut(s) 1988, 2067
MvaI CCWGG 4 cut(s) 22, 1817, 2096, 2969
MvnI CGCG 2 cut(s) 540, 2767
NciI CCSGG 1 cut(s) 2552
NcoI CCATGG 1 cut(s) 2298
NlaIV GGNNCC 4 cut(s) 183, 2827, 2943, 3429
NmuCI GTSAC 6 cut(s) 368, 1042, 1179, 1486, 2413, 3205
NsiI ATGCAT 1 cut(s) 2406
NspI RCATGY 4 cut(s) 178, 529, 1354, 2635
PagI TCATGA 5 cut(s) 820, 826, 1848, 2239, 3454
PciI ACATGT 1 cut(s) 174
PciSI GCTCTTC 3 cut(s) 1515, 2753, 2883
PctI GAATGC 2 cut(s) 1988, 2067
PdmI GAANNNNTTC 1 cut(s) 1888
PflMI CCANNNNNTGG 2 cut(s) 1624, 3005
PfoI TCCNGGA 3 cut(s) 1815, 2094, 2550
PleI GAGTC 2 cut(s) 783, 2072
PpsI GAGTC 2 cut(s) 783, 2072
PscI ACATGT 1 cut(s) 174
PshBI ATTAAT 1 cut(s) 2589
Psp124BI GAGCTC 2 cut(s) 1527, 2694
Psp6I CCWGG 4 cut(s) 20, 1815, 2094, 2967
PspEI GGTNACC 1 cut(s) 2413
PspFI CCCAGC 1 cut(s) 3418
PspGI CCWGG 4 cut(s) 20, 1815, 2094, 2967
PspN4I GGNNCC 4 cut(s) 183, 2827, 2943, 3429
PspPI GGNCC 4 cut(s) 1277, 2825, 3220, 3433
PstI CTGCAG 5 cut(s) 410, 592, 1606, 1904, 2614
PstNI CAGNNNCTG 1 cut(s) 1610
PsuI RGATCY 4 cut(s) 202, 216, 2521, 2941
PvuII CAGCTG 2 cut(s) 2336, 3418
RsaI GTAC 5 cut(s) 659, 1273, 1430, 2654, 3289
RsaNI GTAC 5 cut(s) 658, 1272, 1429, 2653, 3288
RseI CAYNNNNRTG 5 cut(s) 225, 825, 1120, 1263, 2472
SacI GAGCTC 2 cut(s) 1527, 2694
SapI GCTCTTC 3 cut(s) 1515, 2753, 2883
SaqAI TTAA 9 cut(s) 68, 575, 803, 942, 2055, 2127, 2363, 2589, 3105
Sau96I GGNCC 4 cut(s) 1277, 2825, 3220, 3433
ScaI AGTACT 1 cut(s) 659
SchI GAGTC 2 cut(s) 783, 2072
ScrFI CCNGG 5 cut(s) 22, 1817, 2096, 2552, 2969
SduI GDGCHC 4 cut(s) 1365, 1527, 2646, 2694
SfaNI GCATC 6 cut(s) 964, 1912, 2002, 2173, 3010, 3072
SfcI CTRYAG 7 cut(s) 406, 588, 1281, 1602, 1900, 2212, 2610
SinI GGWCC 1 cut(s) 3433
SmiMI CAYNNNNRTG 5 cut(s) 225, 825, 1120, 1263, 2472
SmlI CTYRAG 6 cut(s) 574, 941, 1203, 1329, 1977, 3391
SmoI CTYRAG 6 cut(s) 574, 941, 1203, 1329, 1977, 3391
SsiI CCGC 6 cut(s) 538, 1395, 2318, 2575, 3168, 3275
SstI GAGCTC 2 cut(s) 1527, 2694
StyD4I CCNGG 5 cut(s) 20, 1815, 2094, 2550, 2967
StyI CCWWGG 2 cut(s) 960, 2298
TaaI ACNGT 9 cut(s) 251, 602, 662, 1262, 2216, 2729, 2810, 3235, 3444
TaqI TCGA 6 cut(s) 54, 604, 2207, 2751, 2833, 3018
TatI WGTACW 2 cut(s) 657, 1428
Tru1I TTAA 9 cut(s) 68, 575, 803, 942, 2055, 2127, 2363, 2589, 3105
Tru9I TTAA 9 cut(s) 68, 575, 803, 942, 2055, 2127, 2363, 2589, 3105
TseFI GTSAC 6 cut(s) 368, 1042, 1179, 1486, 2413, 3205
Tsp45I GTSAC 6 cut(s) 368, 1042, 1179, 1486, 2413, 3205
TspGWI ACGGA 3 cut(s) 362, 2881, 3364
Van91I CCANNNNNTGG 2 cut(s) 1624, 3005
Vha464I CTTAAG 2 cut(s) 574, 941
VpaK11BI GGWCC 1 cut(s) 3433
VspI ATTAAT 1 cut(s) 2589
XagI CCTNNNNNAGG 1 cut(s) 2720
XapI RAATTY 4 cut(s) 328, 1137, 1885, 2680
XbaI TCTAGA 5 cut(s) 161, 1009, 2791, 3064, 3149
XceI RCATGY 4 cut(s) 178, 529, 1354, 2635
XcmI CCANNNNNNNNNTGG 3 cut(s) 147, 542, 1621
XmnI GAANNNNTTC 1 cut(s) 1888
ZrmI AGTACT 1 cut(s) 659
Zsp2I ATGCAT 1 cut(s) 2406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.