Rh3BG344500

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0007066
Physical Location & Seq
Forward (+)
37477449 .. 37480313
2865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG344500.1

Sequence Viewer

Length: 2631 bp
ATGGAGGCCAATAAGTTTGCAACCATGTTGAAGAGGAAAAGTCCCAGGTTCGTTCTAGTTCTAGTCTATGCAGTCCTCGAATGGGTGCTGTTAATACTCCTCCTGCTCAACTCTTTTTTCAGTTACTTGATCACAAAGTTTGCCAACTATTTTGGCCTCAAACCACCTTGTCCCTGGTGTTCTTCTAGAGTTGAGCACATGTTGGAACCACAAAAGTCTTACTCAGATCTTGTATGTGAGATCCATGCCAATGAGATATCTAAACTGGGTTACTGTCTCAATCATAAAAGACTGGCTGAATCACATAGTATGTGTGAGAACTGCTTTTCTTCTCAACCAAATTGCAGAGAAAATTCTACTCAAAGTGGGATGGATTTGATTTCATTGCTGAGTGACAAATATCAGAAGAAACCTTCCATCTGTTCTTGCTGCAGTAAGAGCTTCAACACCAAACTCTCTCCTCTTTCATTGGAGTATGCTCAGAAAGGAGATTCAAATATAGAAGCAGTGCATGATGACAACAATGAAGGCACAACCAAACCTGATAGCATGTCAGTCCACAGCGAAAATGGCTATGAGATGGAGACAACTTCTAGCTTAAGAGACACTACTGCAGAAGAAAACTGTCGAAATGAAAGTAAGAAACTTGTCCATCAATCTTCCAATGCTACTACCATTGAGTACTGTTGCCTTCAAGAAGATCACTCTCTTGAAACTACCCCCCTTCCTTCTGAGAGTGGTATGGTCTGTGATCTTGGTTGCAGCCTGATTCCTACTGATTTGATTGATTTTTCAACTACAATAGACGAAGGACTCATAAACAGCTTAAAAGATCAGGAACATCATGATCATGAACAAGGAAGTTTTGATTCCAAGTCAAAGATTAAATTGGAAGATCAACTCTCACGGGAAGCAGCATTACTCATGGTAACTAAGAGTAATGGAAGACTTAAGAGCTTGGAGATGGCCAAGGGATTTACTGATGCTTCATCTATTGAACAAGTGGAAAAGCGAAATCTAGAGTTGGTTGGCAAGCTATGTGATGATTTGGTCACTGCTCCAGAAGCTCAAACTTCGTTCGTTGACACTGAACAACTAACAGATACCAAAGAACCTACCGATCCACCAGAATGTGAAGAAGAGAGAATTTTGGCCGTCATTTCAAGAAATCTGTCAAACACAAAAGGGTCACACCATGCAGCCGATCAGCCTCAAGCTCACAAGGACTCTTCACTGCCATTCCTTGAAATATCCAATGTGCCAGCTCACAGTGATGTCGGTACAGGGCTTACAGGAAAATCAATGTTGCAAGACAAAACAAGTAGAACTAAAAATGCTCAAGAAGTCAACCATTTTTGCATGTGTTCAGAGCCTAGTGAACCTGAGGAAGAGAGGTTGGTATGCGGACAAAATTGTATAAACACTATCCATTACTTGTACAAAAAGCCGAATGGAAATGGAGAATGTGGGGCAGATGATGGGAGTGTGGCAAGTGTGACGAATGGTGGAGAACCTGTAACAACCATTGAAGAGCTCAAAGCAGCATTGAAGGCTGAAAGTAAAGCTTTAAGTGCTTTATATTCAGAACTAGAAGAAGAGAGAAGTGCATCTGCAGTAGCTGCCAACCAGACTATGGCTATGATAACAAGGCTTCAAGAAGAGAAGGCAGCAATGCAGATGGAGGCATTGCAATATCAGAGGATGATGGAAGAACAATCTGAGTATGATCAGGAAGCCTTGCAACTTTTGAATGAGTTGATGAACAAAATGGAGAGGGAAAAACAAGAGCTAGAGAAAGAATTGGAAATATATCAGAAGAAAGTCCTGGATTATGAGGAAAAAGAGAAGATACGGGTCATGAGCAGAATCAAATATGGAAGTATGAGAAGTAGAAATTCTTCTGCTTCCTGCAGCCAATCTTGGGATAGTGATGCGCTATCCATTGATCTCAATTGTGAAGCAAGGGATGAAGATAGTTCTTTCCCTCAAGAATGCAGTAACAACACCAATAATCCAGATGAGGCAGAAGCTCTAAGCTTGGAAGAAATGGCTATGGATTGTGTTAAGCAGATGAGCATTCTTGATGACTCATTGGCAGGCTTCCAGGAAGAGAGGTTGTCAGTTCTGGATCAGCTTAAAGCATTGGAAGATAGGCTAGTAGTGTTGAGTGAAAATGAGAACTGCTCTCAGGATGCTAATTTAGTCAAGAATTGCTCAAACTACAGTACCAAAAACATTGAAGAAAATCATGATTTCAGCAGTCCAGAAGAAAAACAAATATCAGATGAGAAAATAACATTGGATTCCATGGCAAAGAGGCTTCTTCCACTTTTGGGTGTAGCAGCTGAGGATAATGAAACTGTAGCAGGTTTAACAAATGAAAAACAAGTCAGAACCGAATCTATTGCAATGCATAACTCGGTGACCAATTCTGAATTGCCTGATACCAGAATAGCCATTGAGGAGGAGGTGGATCATGTTTATGAGAGGCTACAAGCTCTTGAAAAAAGTAATTTGGAGAAGGGGATGGATCTTCTTCAAGAGAGCTTGCAACATCTCCGGGATTTGAGGGATGTTGAACTCCGCATAAGAAACATTAATGTTCCTGTGGATGAAGCTGCAGGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008092 GO:0017022
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

876

Amino Acids

98.16

Weight (kDa)

4.71

Isoelectric Point (pI)

53.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 511 - 601 9.9e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 2357, 2612
AccB7I CCANNNNNTGG 1 cut(s) 1633
AciI CCGC 2 cut(s) 1404, 2584
AclWI GGATC 5 cut(s) 235, 1115, 2136, 2481, 2538
AcoI YGGCCR 2 cut(s) 966, 1152
AcsI RAATTY 3 cut(s) 352, 1146, 1894
AfaI GTAC 4 cut(s) 683, 1282, 1439, 2227
AfiI CCNNNNNNNGG 4 cut(s) 82, 1633, 1921, 2333
AflII CTTAAG 2 cut(s) 598, 950
AflIII ACRYGT 1 cut(s) 198
AjnI CCWGG 4 cut(s) 44, 173, 1824, 2103
AjuI GAANNNNNNNTTGG 2 cut(s) 2282, 2314
Alw21I GWGCWC 2 cut(s) 198, 1536
Alw26I GTCTC 3 cut(s) 281, 578, 597
AlwI GGATC 5 cut(s) 235, 1115, 2136, 2481, 2538
AlwNI CAGNNNCTG 1 cut(s) 1619
AoxI GGCC 4 cut(s) 6, 154, 966, 1152
ApoI RAATTY 3 cut(s) 352, 1146, 1894
AseI ATTAAT 1 cut(s) 2598
Asp700I GAANNNNTTC 1 cut(s) 1897
AspLEI GCGC 1 cut(s) 1936
AsuC2I CCSGG 1 cut(s) 2561
AsuHPI GGTGA 1 cut(s) 2434
AxyI CCTNAGG 1 cut(s) 1383
BaeI ACNNNNGTAYC 2 cut(s) 2209, 2242
BalI TGGCCA 1 cut(s) 968
BanII GRGCYC 1 cut(s) 1536
BarI GAAGNNNNNNTAC 4 cut(s) 903, 935, 1882, 1914
BbsI GAAGAC 1 cut(s) 952
Bbv12I GWGCWC 2 cut(s) 198, 1536
BbvCI CCTCAGC 1 cut(s) 2346
BccI CCATC 9 cut(s) 364, 425, 574, 660, 958, 1472, 1672, 1699, 2521
BceAI ACGGC 1 cut(s) 1139
BcgI CGANNNNNNTGC 2 cut(s) 2387, 2421
BciT130I CCWGG 4 cut(s) 46, 175, 1826, 2105
BclI TGATCA 3 cut(s) 129, 847, 1726
BcnI CCSGG 1 cut(s) 2561
BcoDI GTCTC 3 cut(s) 281, 578, 597
BfaI CTAG 9 cut(s) 56, 62, 186, 594, 1019, 1374, 1589, 1790, 2156
BfmI CTRYAG 7 cut(s) 430, 612, 1611, 1909, 2221, 2361, 2619
BfrI CTTAAG 2 cut(s) 598, 950
BfuAI ACCTGC 2 cut(s) 2357, 2612
BglII AGATCT 1 cut(s) 226
BmcAI AGTACT 1 cut(s) 683
Bme1390I CCNGG 5 cut(s) 46, 175, 1826, 2105, 2561
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 5 cut(s) 46, 175, 1826, 2105, 2561
BmrI ACTGGG 1 cut(s) 275
BmsI GCATC 4 cut(s) 973, 1616, 1921, 2182
BmuI ACTGGG 1 cut(s) 275
BpiI GAAGAC 1 cut(s) 952
BplI GAGNNNNNCTC 2 cut(s) 2168, 2200
BpmI CTGGAG 1 cut(s) 1044
Bpu10I CCTNAGC 1 cut(s) 2346
BpuEI CTTGAG 3 cut(s) 1197, 1323, 1971
BpuMI CCSGG 1 cut(s) 2561
BsaJI CCNNGG 4 cut(s) 44, 173, 969, 2307
BsaXI ACNNNNNCTCC 2 cut(s) 1500, 1530
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 1633, 1921, 2333
Bse1I ACTGG 2 cut(s) 270, 297
Bse21I CCTNAGG 1 cut(s) 1383
Bse3DI GCAATG 4 cut(s) 383, 1677, 1685, 2415
BseBI CCWGG 4 cut(s) 46, 175, 1826, 2105
BseDI CCNNGG 4 cut(s) 44, 173, 969, 2307
BseGI GGATG 7 cut(s) 375, 1707, 1972, 2197, 2532, 2578, 2617
BseLI CCNNNNNNNGG 4 cut(s) 82, 1633, 1921, 2333
BseMI GCAATG 4 cut(s) 383, 1677, 1685, 2415
BseMII CTCAG 8 cut(s) 237, 380, 494, 723, 1374, 1710, 2201, 2337
BseNI ACTGG 2 cut(s) 270, 297
BseRI GAGGAG 4 cut(s) 89, 450, 2477, 2480
BshFI GGCC 4 cut(s) 8, 156, 968, 1154
BsiHKAI GWGCWC 2 cut(s) 198, 1536
BsiSI CCGG 1 cut(s) 2560
BslFI GGGAC 2 cut(s) 27, 156
BslI CCNNNNNNNGG 4 cut(s) 82, 1633, 1921, 2333
BsmAI GTCTC 3 cut(s) 281, 578, 597
BsmFI GGGAC 2 cut(s) 27, 156
BsmI GAATGC 2 cut(s) 1997, 2076
BsnI GGCC 4 cut(s) 8, 156, 968, 1154
Bsp1286I GDGCHC 2 cut(s) 198, 1536
Bsp1407I TGTACA 1 cut(s) 1437
Bsp19I CCATGG 1 cut(s) 2307
BspACI CCGC 2 cut(s) 1404, 2584
BspANI GGCC 4 cut(s) 8, 156, 968, 1154
BspCNI CTCAG 8 cut(s) 236, 381, 493, 724, 1375, 1711, 2200, 2338
BspHI TCATGA 4 cut(s) 844, 850, 1857, 2248
BspLI GGNNCC 1 cut(s) 207
BspMAI CTGCAG 5 cut(s) 434, 616, 1615, 1913, 2623
BspMI ACCTGC 2 cut(s) 2357, 2612
BspPI GGATC 5 cut(s) 235, 1115, 2136, 2481, 2538
BspQI GCTCTTC 1 cut(s) 1524
BspTI CTTAAG 2 cut(s) 598, 950
BsrDI GCAATG 4 cut(s) 383, 1677, 1685, 2415
BsrGI TGTACA 1 cut(s) 1437
BsrI ACTGG 2 cut(s) 270, 297
BssECI CCNNGG 4 cut(s) 44, 173, 969, 2307
BssT1I CCWWGG 2 cut(s) 969, 2307
Bst2UI CCWGG 4 cut(s) 46, 175, 1826, 2105
Bst4CI ACNGT 6 cut(s) 275, 626, 686, 1271, 2225, 2362
Bst6I CTCTTC 8 cut(s) 26, 1134, 1234, 1383, 1524, 1590, 1653, 2103
BstAFI CTTAAG 2 cut(s) 598, 950
BstAPI GCANNNNNTGC 1 cut(s) 1619
BstAUI TGTACA 1 cut(s) 1437
BstC8I GCNNGC 4 cut(s) 1034, 1263, 2098, 2549
BstDSI CCRYGG 1 cut(s) 2307
BstEII GGTNACC 1 cut(s) 2422
BstF5I GGATG 7 cut(s) 375, 1707, 1972, 2197, 2532, 2578, 2617
BstHHI GCGC 1 cut(s) 1936
BstMAI GTCTC 3 cut(s) 281, 578, 597
BstMWI GCNNNNNNNGC 6 cut(s) 438, 570, 1064, 1550, 1571, 1619
BstNI CCWGG 4 cut(s) 46, 175, 1826, 2105
BstNSI RCATGY 3 cut(s) 202, 553, 1363
BstPI GGTNACC 1 cut(s) 2422
BstSCI CCNGG 5 cut(s) 44, 173, 1824, 2103, 2559
BstSFI CTRYAG 7 cut(s) 430, 612, 1611, 1909, 2221, 2361, 2619
BstV2I GAAGAC 1 cut(s) 952
BstX2I RGATCY 3 cut(s) 226, 240, 2530
BstYI RGATCY 3 cut(s) 226, 240, 2530
Bsu36I CCTNAGG 1 cut(s) 1383
BsuRI GGCC 4 cut(s) 8, 156, 968, 1154
BtgI CCRYGG 1 cut(s) 2307
BtsCI GGATG 7 cut(s) 375, 1707, 1972, 2197, 2532, 2578, 2617
BtsI GCAGTG 3 cut(s) 513, 1053, 1232
BtsIMutI CAGTG 5 cut(s) 513, 1053, 1086, 1232, 1276
BveI ACCTGC 2 cut(s) 2357, 2612
Cac8I GCNNGC 4 cut(s) 1034, 1263, 2098, 2549
CaiI CAGNNNCTG 1 cut(s) 1619
CciI TCATGA 4 cut(s) 844, 850, 1857, 2248
CfoI GCGC 1 cut(s) 1936
Csp6I GTAC 4 cut(s) 682, 1281, 1438, 2226
CviQI GTAC 4 cut(s) 682, 1281, 1438, 2226
EaeI YGGCCR 2 cut(s) 966, 1152
Eam1104I CTCTTC 8 cut(s) 26, 1134, 1234, 1383, 1524, 1590, 1653, 2103
EarI CTCTTC 8 cut(s) 26, 1134, 1234, 1383, 1524, 1590, 1653, 2103
Ecl136II GAGCTC 1 cut(s) 1534
Eco130I CCWWGG 2 cut(s) 969, 2307
Eco24I GRGCYC 1 cut(s) 1536
Eco32I GATATC 1 cut(s) 258
Eco53kI GAGCTC 1 cut(s) 1534
Eco81I CCTNAGG 1 cut(s) 1383
Eco91I GGTNACC 1 cut(s) 2422
EcoICRI GAGCTC 1 cut(s) 1534
EcoO65I GGTNACC 1 cut(s) 2422
EcoRII CCWGG 4 cut(s) 44, 173, 1824, 2103
EcoRV GATATC 1 cut(s) 258
EcoT14I CCWWGG 2 cut(s) 969, 2307
EcoT22I ATGCAT 1 cut(s) 2415
EcoT38I GRGCYC 1 cut(s) 1536
ErhI CCWWGG 2 cut(s) 969, 2307
FalI AAGNNNNNCTT 2 cut(s) 1549, 1581
FaqI GGGAC 2 cut(s) 27, 156
FbaI TGATCA 3 cut(s) 129, 847, 1726
FokI GGATG 7 cut(s) 382, 1714, 1979, 2204, 2539, 2585, 2624
FriOI GRGCYC 1 cut(s) 1536
FspBI CTAG 9 cut(s) 56, 62, 186, 594, 1019, 1374, 1589, 1790, 2156
GlaI GCGC 1 cut(s) 1935
GsuI CTGGAG 1 cut(s) 1044
HaeIII GGCC 4 cut(s) 8, 156, 968, 1154
HapII CCGG 1 cut(s) 2560
HhaI GCGC 1 cut(s) 1936
Hin6I GCGC 1 cut(s) 1934
HinP1I GCGC 1 cut(s) 1934
HincII GTYRAC 2 cut(s) 1084, 1348
HindII GTYRAC 2 cut(s) 1084, 1348
HindIII AAGCTT 2 cut(s) 1563, 2035
HpaII CCGG 1 cut(s) 2560
HphI GGTGA 1 cut(s) 2434
Hpy166II GTNNAC 4 cut(s) 559, 1084, 1348, 1379
Hpy8I GTNNAC 4 cut(s) 559, 1084, 1348, 1379
HpyAV CCTTC 9 cut(s) 423, 521, 701, 734, 738, 803, 1543, 1657, 2515
HpyCH4III ACNGT 6 cut(s) 275, 626, 686, 1271, 2225, 2362
HpyF10VI GCNNNNNNNGC 6 cut(s) 438, 570, 1064, 1550, 1571, 1619
HspAI GCGC 1 cut(s) 1934
Ksp22I TGATCA 3 cut(s) 129, 847, 1726
LguI GCTCTTC 1 cut(s) 1524
LmnI GCTCC 1 cut(s) 1063
LweI GCATC 4 cut(s) 973, 1616, 1921, 2182
MaeI CTAG 9 cut(s) 56, 62, 186, 594, 1019, 1374, 1589, 1790, 2156
MfeI CAATTG 1 cut(s) 1951
MflI RGATCY 3 cut(s) 226, 240, 2530
MhlI GDGCHC 2 cut(s) 198, 1536
MlsI TGGCCA 1 cut(s) 968
MluNI TGGCCA 1 cut(s) 968
MlyI GAGTC 3 cut(s) 807, 1220, 2081
MmeI TCCRAC 1 cut(s) 183
Mox20I TGGCCA 1 cut(s) 968
Mph1103I ATGCAT 1 cut(s) 2415
MroXI GAANNNNTTC 1 cut(s) 1897
MscI TGGCCA 1 cut(s) 968
MslI CAYNNNNRTG 5 cut(s) 249, 849, 1129, 1272, 2481
Msp20I TGGCCA 1 cut(s) 968
MspA1I CMGCKG 1 cut(s) 2345
MspCI CTTAAG 2 cut(s) 598, 950
MspI CCGG 1 cut(s) 2560
MspR9I CCNGG 5 cut(s) 46, 175, 1826, 2105, 2561
MunI CAATTG 1 cut(s) 1951
Mva1269I GAATGC 2 cut(s) 1997, 2076
MvaI CCWGG 4 cut(s) 46, 175, 1826, 2105
MwoI GCNNNNNNNGC 6 cut(s) 438, 570, 1064, 1550, 1571, 1619
NciI CCSGG 1 cut(s) 2561
NcoI CCATGG 1 cut(s) 2307
NlaIV GGNNCC 1 cut(s) 207
NmuCI GTSAC 5 cut(s) 392, 1051, 1188, 1495, 2422
NsiI ATGCAT 1 cut(s) 2415
NspI RCATGY 3 cut(s) 202, 553, 1363
PagI TCATGA 4 cut(s) 844, 850, 1857, 2248
PciI ACATGT 1 cut(s) 198
PciSI GCTCTTC 1 cut(s) 1524
PctI GAATGC 2 cut(s) 1997, 2076
PdmI GAANNNNTTC 1 cut(s) 1897
PfeI GAWTC 7 cut(s) 299, 491, 769, 869, 1866, 2303, 2399
PflMI CCANNNNNTGG 1 cut(s) 1633
PfoI TCCNGGA 3 cut(s) 1824, 2103, 2559
PleI GAGTC 3 cut(s) 807, 1220, 2081
PpsI GAGTC 3 cut(s) 807, 1220, 2081
PscI ACATGT 1 cut(s) 198
PshBI ATTAAT 1 cut(s) 2598
Psp124BI GAGCTC 1 cut(s) 1536
Psp6I CCWGG 4 cut(s) 44, 173, 1824, 2103
PspEI GGTNACC 1 cut(s) 2422
PspGI CCWGG 4 cut(s) 44, 173, 1824, 2103
PspN4I GGNNCC 1 cut(s) 207
PstI CTGCAG 5 cut(s) 434, 616, 1615, 1913, 2623
PstNI CAGNNNCTG 1 cut(s) 1619
PsuI RGATCY 3 cut(s) 226, 240, 2530
PvuII CAGCTG 1 cut(s) 2345
RsaI GTAC 4 cut(s) 683, 1282, 1439, 2227
RsaNI GTAC 4 cut(s) 682, 1281, 1438, 2226
RseI CAYNNNNRTG 5 cut(s) 249, 849, 1129, 1272, 2481
SacI GAGCTC 1 cut(s) 1536
SapI GCTCTTC 1 cut(s) 1524
ScaI AGTACT 1 cut(s) 683
SchI GAGTC 3 cut(s) 807, 1220, 2081
ScrFI CCNGG 5 cut(s) 46, 175, 1826, 2105, 2561
SduI GDGCHC 2 cut(s) 198, 1536
SfaNI GCATC 4 cut(s) 973, 1616, 1921, 2182
SfcI CTRYAG 7 cut(s) 430, 612, 1611, 1909, 2221, 2361, 2619
SmiMI CAYNNNNRTG 5 cut(s) 249, 849, 1129, 1272, 2481
SmlI CTYRAG 5 cut(s) 598, 950, 1212, 1338, 1986
SmoI CTYRAG 5 cut(s) 598, 950, 1212, 1338, 1986
SsiI CCGC 2 cut(s) 1404, 2584
SspMI CTAG 9 cut(s) 56, 62, 186, 594, 1019, 1374, 1589, 1790, 2156
SstI GAGCTC 1 cut(s) 1536
StyD4I CCNGG 5 cut(s) 44, 173, 1824, 2103, 2559
StyI CCWWGG 2 cut(s) 969, 2307
TaaI ACNGT 6 cut(s) 275, 626, 686, 1271, 2225, 2362
TaqI TCGA 2 cut(s) 78, 628
TatI WGTACW 2 cut(s) 681, 1437
TfiI GAWTC 7 cut(s) 299, 491, 769, 869, 1866, 2303, 2399
TscAI CASTG 5 cut(s) 513, 1060, 1093, 1239, 1276
TseFI GTSAC 5 cut(s) 392, 1051, 1188, 1495, 2422
Tsp45I GTSAC 5 cut(s) 392, 1051, 1188, 1495, 2422
TspRI CASTG 5 cut(s) 513, 1060, 1093, 1239, 1276
Van91I CCANNNNNTGG 1 cut(s) 1633
Vha464I CTTAAG 2 cut(s) 598, 950
VspI ATTAAT 1 cut(s) 2598
XapI RAATTY 3 cut(s) 352, 1146, 1894
XbaI TCTAGA 2 cut(s) 185, 1018
XceI RCATGY 3 cut(s) 202, 553, 1363
XcmI CCANNNNNNNNNTGG 3 cut(s) 171, 566, 1630
XmnI GAANNNNTTC 1 cut(s) 1897
XspI CTAG 9 cut(s) 56, 62, 186, 594, 1019, 1374, 1589, 1790, 2156
ZrmI AGTACT 1 cut(s) 683
Zsp2I ATGCAT 1 cut(s) 2415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.