MD09G1263700.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007066
Physical Location & Seq
Reverse (-)
33683707 .. 33687888
4182 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1263700.v1.1.491

Sequence Viewer

Length: 2670 bp
ATGGCAACCAACAAGTTTGCAAGCATGTTGCACAGGAACACTCCCAAGTTGATTGTAATTCTAGTCTATGCTGTTCTTGAATGGATTTTGATAATACTCCTCCTCCTCAACTCTTTGCTCGGTTACTTGATCAACAAATATGCCAACTACTTCGGCCTGAAACCACCTTGTCCGTTGTGTACTCCTAGAATCGAAGGCGTCTTGGAACAAGGCAAGAGCTCAAACGACTCTTATACAGATGTTGTATGTCAGAGCCATGCCACTCAGATATCCAATATGTTTCACCGTCCTTGTTCCGAACCTTGTTGCGGTTCTTTGGAGTATGCGCGGAAAGGGAATTCAGCTGTAGAAGCTGTGGATGATGGAAACAATGGAGGTCAGTATCAAAGTGCAAATAAACCCGATAGCCTGTCAACTCACAGTGACAATGGCTATGAGTTGGAGACAACGAATGAAGAACATGAGGACGACAGAGTGGCAGACGGGCAACAACTTACATCGGATGTTTGCAGTTCTAGCTTCGGAGGCATTGCAGTGGAAGATTGTCCAAGGTCTAGATCAGTATTGCTATGTCAAGAAAATGATGCAGATGAAGATAACAAAGGAGGTTCTCTTGACATTGCGCGGTCAGATTCTAACAGCACGAAACTTGTCCATCGATCTTCCGATGCTAGTGCTACTAGCATTCAGTGTTGCTTTGAAGAAGATTACTCTCTTGAAATCATCCCCCTATCTTGCGAGAATGGTTTGATCTATGCTCACAATCATCGGTTGATTCCCGTTGAGTTGATCGATTTTTCAACTACAGTAGACCAAGTAACCCGCAATGTAAAAGAGGAGGAGGTGAGAGAGCATGATCATAAAGAGTTAAATTTCGCTTCTGAGTCAAACATCGGATCTGAACGTCAACTCTCCGGAGAAGCATCATTAGTACTTGTGGCAAACGAGAGTGCAGTTAACACAGGTAATGGAGAAGTCGAAAGCTTAGATATGTCCAGGGGTGTTACTGATAACTCTTCTGACGTAGACGCAGAAGAGGGAAAACAAGATTTGGTTCGCAAGCCATGTGACGAAGTTGTCACTGCTCCAGGAGCTCAAATATTATTTGTTCGAATTGAAGAACCCGATAAGAAAGAACGAAATGATCCACCAGCATCTGAAGAAGAAAGTATTTTTGCGAACACAGAAGAGTATGACCGTGCAAGTGATCACCCTCGGGCTCAAGAAGGTTCTTCTTCGCCGTGCTTTGAAATTCCCAATGTACCTGATACATTCTCGGCTCAGAGTGATTACGGTACAAGGCATGCTGTAGCAGCTACACCGCAAGGAGAAACAAGTATGCCTGAAAAGGCTCAAGAACGAGAGGAAGAGGAGATTCCTGACTCACCAACATCTGTAAACAGTCTTTGTTCCTTGCACAAGAAACCAGATGATAAAATGGAGTCGGCAGTGGAAGAGTATTTGGATGGAAGTGCTGTGAGTGAGACAGAAGAGAGGGATCCTTTTACAACCATTGGACGGCTCAAAGAAGCGCTGACAGCTGAGCGCAAATCTCTAAGTTCTTTATATGCAGAACTAGAGGAAGAGAGGAGTGCGTCTGCAATTGCCGCCAACCAGACTATGGCTATGATAACAAGGCTTCAAGAAGAGAAGGCAGCAATGCAAATGGAGGCATTGCAATACCAGAGGATGATGGAAGAACAATCCGAGTACGACCAGGACGCCTTACAACTTCTGAATGATCTGATGAACAAGAAGGAGAAGGAAAAGCAAGAACTAGAGAGAGAATTGGAAATATATCAAAAGAAAGTCCAAGATTATGAGGAAAAAGAGAAGGCAAGGACCATGAGCAGAATTAAATATGGAAGTATAAGAAGTAGGAATTCTTCTGCTTCTTGCAGCCAGTCATGGGATAGTGATGCGCTATCGATTGATCTTAATGTTGAAGCGAGGGACGAAGAAAGTGGCTTCGGTGGCCATCAAGAAAGCAATGACAAAAACAATACCACAGACGATTCAGGTCTGAGTTTGGAAGATTTGGCTCTGGATTGTGTTAAAAATATGAGTGTTCTTGATGAATCACTAGCAGGATTCGAGGAAGAGAGGCTGTCCATTCTGGATCAGCTCAAAGCCTTGGAGGAGAAGCTAATCACATTGGGTGCAAATGAGGAATTCGCCGAGGACTTTAAGCTAGTTGAGCATTCCCCAACGTACAGCCTCAAATACTCCGAAGAGAATCATGATGTTAGCAGTCCAGAAGAAAATGGGATAGATAGATATCATCCCGAGGAAAAGACATTGGATTCCATGGCAAAGAGGCTTCTTCCACTTTTGGATGCAACTGACAATGAAAGCGAAGAAGGGTTAACTCATGAAGAACACGTTGATCAATCAGAATCAATTGAAATAATGCAGAACTCAATTACCAATTTCGAATTGAATGATGGAAAAATAGCCATTGAAGAGGAGGTGGATCATGTATATGAGAGGCTACAAGCTCTTGAAGCAGACAGGGAGTTTCTCAAGCACTGCATGAGCTCCATGAAGAAGGGAGACAAAGGGGTGGATCTTCTCCAAGAGATTTTGCAACATCTGCGGGATCTGAGGGTTGTTGAACTCCGCGTCAAGAACATGAGTGATCCCGAGGGCGATGAAGTTGCAGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

890

Amino Acids

99.33

Weight (kDa)

4.46

Isoelectric Point (pI)

54.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 507 - 597 7.1e-33 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1076
AccB7I CCANNNNNTGG 1 cut(s) 1621
AccI GTMKAC 2 cut(s) 810, 1026
AccII CGCG 3 cut(s) 328, 625, 2622
AccIII TCCGGA 1 cut(s) 914
AciI CCGC 8 cut(s) 309, 328, 625, 823, 1322, 1608, 2596, 2620
AclWI GGATC 9 cut(s) 904, 1139, 1493, 1506, 2127, 2481, 2574, 2607, 2633
AcoI YGGCCR 1 cut(s) 1975
AcsI RAATTY 5 cut(s) 337, 871, 1251, 1882, 2171
AcuI CTGAAG 1 cut(s) 1179
AcyI GRCGYC 2 cut(s) 198, 1722
AfaI GTAC 6 cut(s) 181, 933, 1263, 1297, 1712, 2213
AfeI AGCGCT 1 cut(s) 1533
AfiI CCNNNNNNNGG 4 cut(s) 308, 1518, 1621, 1909
AflIII ACRYGT 1 cut(s) 2380
AjnI CCWGG 3 cut(s) 995, 1087, 1716
Alw21I GWGCWC 3 cut(s) 221, 1096, 2540
Alw26I GTCTC 3 cut(s) 437, 1478, 2547
AlwI GGATC 9 cut(s) 904, 1139, 1493, 1506, 2127, 2481, 2574, 2607, 2633
AlwNI CAGNNNCTG 1 cut(s) 1157
Ama87I CYCGRG 3 cut(s) 1215, 2285, 2642
Aor13HI TCCGGA 1 cut(s) 914
Aor51HI AGCGCT 1 cut(s) 1533
AoxI GGCC 2 cut(s) 154, 1975
ApeKI GCWGC 3 cut(s) 1313, 1655, 1899
ApoI RAATTY 5 cut(s) 337, 871, 1251, 1882, 2171
AspLEI GCGC 5 cut(s) 328, 625, 1534, 1548, 1924
AspS9I GGNCC 1 cut(s) 1842
AsuHPI GGTGA 4 cut(s) 275, 856, 1202, 1377
AsuII TTCGAA 2 cut(s) 1111, 2433
AvaI CYCGRG 3 cut(s) 1215, 2285, 2642
AvaII GGWCC 1 cut(s) 1842
BalI TGGCCA 1 cut(s) 1977
BamHI GGATCC 1 cut(s) 1498
BanII GRGCYC 4 cut(s) 221, 1096, 1222, 2540
BarI GAAGNNNNNNTAC 2 cut(s) 1870, 1902
Bbv12I GWGCWC 3 cut(s) 221, 1096, 2540
BbvI GCAGC 3 cut(s) 1325, 1667, 1911
BccI CCATC 6 cut(s) 356, 663, 1460, 1687, 1986, 2438
BceAI ACGGC 2 cut(s) 1225, 1535
BcgI CGANNNNNNTGC 3 cut(s) 656, 690, 2639
BciT130I CCWGG 3 cut(s) 997, 1089, 1718
BclI TGATCA 4 cut(s) 129, 856, 1207, 2386
BcoDI GTCTC 3 cut(s) 437, 1478, 2547
BfmI CTRYAG 3 cut(s) 345, 804, 1308
BfoI RGCGCY 1 cut(s) 1535
BisI GCNGC 4 cut(s) 1314, 1608, 1656, 1900
BlpI GCTNAGC 1 cut(s) 1542
BlsI GCNGC 4 cut(s) 1315, 1609, 1657, 1901
BmcAI AGTACT 1 cut(s) 933
Bme1390I CCNGG 3 cut(s) 997, 1089, 1718
Bme18I GGWCC 1 cut(s) 1842
BmeT110I CYCGRG 3 cut(s) 1215, 2285, 2642
BmgT120I GGNCC 1 cut(s) 1842
BmiI GGNNCC 1 cut(s) 1500
BmrFI CCNGG 3 cut(s) 997, 1089, 1718
BmsI GCATC 6 cut(s) 574, 658, 932, 1163, 1909, 2326
BpmI CTGGAG 1 cut(s) 1071
Bpu1102I GCTNAGC 1 cut(s) 1542
Bpu14I TTCGAA 2 cut(s) 1111, 2433
BpuEI CTTGAG 3 cut(s) 1206, 1338, 2507
Bsa29I ATCGAT 3 cut(s) 658, 792, 1928
BsaBI GATNNNNATC 1 cut(s) 2277
BsaHI GRCGYC 2 cut(s) 198, 1722
BsaJI CCNNGG 8 cut(s) 548, 996, 1214, 2133, 2178, 2286, 2307, 2643
BsaWI WCCGGW 1 cut(s) 914
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 4 cut(s) 308, 1518, 1621, 1909
Bse1I ACTGG 1 cut(s) 1903
Bse3DI GCAATG 6 cut(s) 528, 618, 832, 1665, 1673, 1996
Bse8I GATNNNNATC 1 cut(s) 2277
BseAI TCCGGA 1 cut(s) 914
BseBI CCWGG 3 cut(s) 997, 1089, 1718
BseCI ATCGAT 3 cut(s) 658, 792, 1928
BseDI CCNNGG 8 cut(s) 548, 996, 1214, 2133, 2178, 2286, 2307, 2643
BseGI GGATG 7 cut(s) 364, 508, 723, 1471, 1695, 2281, 2341
BseJI GATNNNNATC 1 cut(s) 2277
BseLI CCNNNNNNNGG 4 cut(s) 308, 1518, 1621, 1909
BseMI GCAATG 6 cut(s) 528, 618, 832, 1665, 1673, 1996
BseMII CTCAG 6 cut(s) 278, 873, 1295, 1533, 2015, 2594
BseNI ACTGG 1 cut(s) 1903
BseRI GAGGAG 9 cut(s) 89, 92, 95, 851, 854, 1385, 1603, 2153, 2480
BseXI GCAGC 3 cut(s) 1325, 1667, 1911
BsgI GTGCAG 1 cut(s) 972
Bsh1236I CGCG 3 cut(s) 328, 625, 2622
BshFI GGCC 2 cut(s) 156, 1977
BshVI ATCGAT 3 cut(s) 658, 792, 1928
BsiHKAI GWGCWC 3 cut(s) 221, 1096, 2540
BsiHKCI CYCGRG 3 cut(s) 1215, 2285, 2642
BsiSI CCGG 1 cut(s) 915
BslFI GGGAC 1 cut(s) 1967
BslI CCNNNNNNNGG 4 cut(s) 308, 1518, 1621, 1909
BsmAI GTCTC 3 cut(s) 437, 1478, 2547
BsmFI GGGAC 1 cut(s) 1967
BsmI GAATGC 2 cut(s) 684, 2200
BsnI GGCC 2 cut(s) 156, 1977
BsoBI CYCGRG 3 cut(s) 1215, 2285, 2642
Bsp119I TTCGAA 2 cut(s) 1111, 2433
Bsp1286I GDGCHC 4 cut(s) 221, 1096, 1222, 2540
Bsp13I TCCGGA 1 cut(s) 914
Bsp1720I GCTNAGC 1 cut(s) 1542
Bsp19I CCATGG 1 cut(s) 2307
BspACI CCGC 8 cut(s) 309, 328, 625, 823, 1322, 1608, 2596, 2620
BspANI GGCC 2 cut(s) 156, 1977
BspCNI CTCAG 6 cut(s) 277, 874, 1294, 1534, 2016, 2595
BspDI ATCGAT 3 cut(s) 658, 792, 1928
BspEI TCCGGA 1 cut(s) 914
BspFNI CGCG 3 cut(s) 328, 625, 2622
BspHI TCATGA 2 cut(s) 2239, 2371
BspLI GGNNCC 1 cut(s) 1500
BspPI GGATC 9 cut(s) 904, 1139, 1493, 1506, 2127, 2481, 2574, 2607, 2633
BspT104I TTCGAA 2 cut(s) 1111, 2433
BsrDI GCAATG 6 cut(s) 528, 618, 832, 1665, 1673, 1996
BsrI ACTGG 1 cut(s) 1903
BssECI CCNNGG 8 cut(s) 548, 996, 1214, 2133, 2178, 2286, 2307, 2643
BssNI GRCGYC 2 cut(s) 198, 1722
BssT1I CCWWGG 3 cut(s) 548, 2133, 2307
Bst2UI CCWGG 3 cut(s) 997, 1089, 1718
Bst4CI ACNGT 6 cut(s) 287, 422, 808, 1199, 1295, 1403
BstACI GRCGYC 2 cut(s) 198, 1722
BstAPI GCANNNNNTGC 1 cut(s) 2593
BstBI TTCGAA 2 cut(s) 1111, 2433
BstC8I GCNNGC 3 cut(s) 22, 1061, 1305
BstDEI CTNAG 8 cut(s) 264, 882, 985, 1281, 1542, 1556, 2024, 2603
BstDSI CCRYGG 1 cut(s) 2307
BstF5I GGATG 7 cut(s) 364, 508, 723, 1471, 1695, 2281, 2341
BstFNI CGCG 3 cut(s) 328, 625, 2622
BstH2I RGCGCY 1 cut(s) 1535
BstHHI GCGC 5 cut(s) 328, 625, 1534, 1548, 1924
BstMAI GTCTC 3 cut(s) 437, 1478, 2547
BstNI CCWGG 3 cut(s) 997, 1089, 1718
BstNSI RCATGY 2 cut(s) 28, 1307
BstSCI CCNGG 3 cut(s) 995, 1087, 1716
BstSFI CTRYAG 3 cut(s) 345, 804, 1308
BstUI CGCG 3 cut(s) 328, 625, 2622
BstV1I GCAGC 3 cut(s) 1325, 1667, 1911
BstX2I RGATCY 4 cut(s) 896, 1498, 2566, 2599
BstYI RGATCY 4 cut(s) 896, 1498, 2566, 2599
Bsu15I ATCGAT 3 cut(s) 658, 792, 1928
BsuRI GGCC 2 cut(s) 156, 1977
BsuTUI ATCGAT 3 cut(s) 658, 792, 1928
BtgI CCRYGG 1 cut(s) 2307
BtgZI GCGATG 1 cut(s) 2664
BtsCI GGATG 7 cut(s) 364, 508, 723, 1471, 1695, 2281, 2341
BtsI GCAGTG 4 cut(s) 540, 1080, 1455, 2527
BtsIMutI CAGTG 6 cut(s) 427, 540, 695, 1080, 1455, 2527
Cac8I GCNNGC 3 cut(s) 22, 1061, 1305
CaiI CAGNNNCTG 1 cut(s) 1157
CciI TCATGA 2 cut(s) 2239, 2371
CfoI GCGC 5 cut(s) 328, 625, 1534, 1548, 1924
Cfr13I GGNCC 1 cut(s) 1842
ClaI ATCGAT 3 cut(s) 658, 792, 1928
CseI GACGC 5 cut(s) 187, 1037, 1584, 1730, 2611
Csp6I GTAC 6 cut(s) 180, 932, 1262, 1296, 1711, 2212
CviQI GTAC 6 cut(s) 180, 932, 1262, 1296, 1711, 2212
DdeI CTNAG 8 cut(s) 264, 882, 985, 1281, 1542, 1556, 2024, 2603
DrdI GACNNNNNNGTC 1 cut(s) 1076
DseDI GACNNNNNNGTC 1 cut(s) 1076
EaeI YGGCCR 1 cut(s) 1975
Ecl136II GAGCTC 3 cut(s) 219, 1094, 2538
Eco130I CCWWGG 3 cut(s) 548, 2133, 2307
Eco24I GRGCYC 4 cut(s) 221, 1096, 1222, 2540
Eco32I GATATC 2 cut(s) 270, 2279
Eco47I GGWCC 1 cut(s) 1842
Eco47III AGCGCT 1 cut(s) 1533
Eco53kI GAGCTC 3 cut(s) 219, 1094, 2538
Eco57I CTGAAG 1 cut(s) 1179
Eco88I CYCGRG 3 cut(s) 1215, 2285, 2642
EcoICRI GAGCTC 3 cut(s) 219, 1094, 2538
EcoRI GAATTC 3 cut(s) 337, 1882, 2171
EcoRII CCWGG 3 cut(s) 995, 1087, 1716
EcoRV GATATC 2 cut(s) 270, 2279
EcoT14I CCWWGG 3 cut(s) 548, 2133, 2307
EcoT38I GRGCYC 4 cut(s) 221, 1096, 1222, 2540
ErhI CCWWGG 3 cut(s) 548, 2133, 2307
FaqI GGGAC 1 cut(s) 1967
FauI CCCGC 2 cut(s) 830, 2589
FbaI TGATCA 4 cut(s) 129, 856, 1207, 2386
FblI GTMKAC 2 cut(s) 810, 1026
Fnu4HI GCNGC 4 cut(s) 1314, 1608, 1656, 1900
FokI GGATG 7 cut(s) 371, 515, 710, 1478, 1702, 2268, 2348
FriOI GRGCYC 4 cut(s) 221, 1096, 1222, 2540
Fsp4HI GCNGC 4 cut(s) 1314, 1608, 1656, 1900
GlaI GCGC 5 cut(s) 327, 624, 1533, 1547, 1923
GluI GCNGC 4 cut(s) 1314, 1608, 1656, 1900
GsuI CTGGAG 1 cut(s) 1071
HaeII RGCGCY 1 cut(s) 1535
HaeIII GGCC 2 cut(s) 156, 1977
HapII CCGG 1 cut(s) 915
HgaI GACGC 5 cut(s) 187, 1037, 1584, 1730, 2611
HhaI GCGC 5 cut(s) 328, 625, 1534, 1548, 1924
Hin1I GRCGYC 2 cut(s) 198, 1722
Hin6I GCGC 5 cut(s) 326, 623, 1532, 1546, 1922
HinP1I GCGC 5 cut(s) 326, 623, 1532, 1546, 1922
HincII GTYRAC 4 cut(s) 414, 908, 958, 2367
HindII GTYRAC 4 cut(s) 414, 908, 958, 2367
HindIII AAGCTT 1 cut(s) 982
HpaI GTTAAC 2 cut(s) 958, 2367
HpaII CCGG 1 cut(s) 915
HphI GGTGA 4 cut(s) 275, 856, 1202, 1377
Hpy166II GTNNAC 8 cut(s) 180, 414, 811, 908, 958, 1027, 1399, 2367
Hpy8I GTNNAC 8 cut(s) 180, 414, 811, 908, 958, 1027, 1399, 2367
HpyAV CCTTC 8 cut(s) 188, 1220, 1645, 1750, 1756, 1828, 2354, 2542
HpyCH4III ACNGT 6 cut(s) 287, 422, 808, 1199, 1295, 1403
HpyCH4IV ACGT 4 cut(s) 904, 1023, 2210, 2382
HpyF3I CTNAG 8 cut(s) 264, 882, 985, 1281, 1542, 1556, 2024, 2603
HpySE526I ACGT 4 cut(s) 904, 1023, 2210, 2382
Hsp92I GRCGYC 2 cut(s) 198, 1722
HspAI GCGC 5 cut(s) 326, 623, 1532, 1546, 1922
Kpn2I TCCGGA 1 cut(s) 914
Ksp22I TGATCA 4 cut(s) 129, 856, 1207, 2386
KspAI GTTAAC 2 cut(s) 958, 2367
LmnI GCTCC 3 cut(s) 1090, 1091, 2543
Lsp1109I GCAGC 3 cut(s) 1325, 1667, 1911
LweI GCATC 6 cut(s) 574, 658, 932, 1163, 1909, 2326
MaeII ACGT 4 cut(s) 904, 1023, 2210, 2382
MaeIII GTNAC 6 cut(s) 122, 422, 817, 1003, 1067, 1078
MfeI CAATTG 2 cut(s) 1602, 2400
MflI RGATCY 4 cut(s) 896, 1498, 2566, 2599
MhlI GDGCHC 4 cut(s) 221, 1096, 1222, 2540
MlsI TGGCCA 1 cut(s) 1977
MluNI TGGCCA 1 cut(s) 1977
MlyI GAGTC 4 cut(s) 221, 893, 1376, 1451
MmeI TCCRAC 1 cut(s) 420
Mox20I TGGCCA 1 cut(s) 1977
MroI TCCGGA 1 cut(s) 914
MscI TGGCCA 1 cut(s) 1977
MseI TTAA 7 cut(s) 869, 957, 1857, 1938, 2055, 2187, 2366
MslI CAYNNNNRTG 2 cut(s) 533, 2481
Msp20I TGGCCA 1 cut(s) 1977
MspA1I CMGCKG 2 cut(s) 344, 1541
MspI CCGG 1 cut(s) 915
MspR9I CCNGG 3 cut(s) 997, 1089, 1718
MunI CAATTG 2 cut(s) 1602, 2400
Mva1269I GAATGC 2 cut(s) 684, 2200
MvaI CCWGG 3 cut(s) 997, 1089, 1718
MvnI CGCG 3 cut(s) 328, 625, 2622
NcoI CCATGG 1 cut(s) 2307
NlaIV GGNNCC 1 cut(s) 1500
NmeAIII GCCGAG 2 cut(s) 1256, 2203
NmuCI GTSAC 3 cut(s) 422, 1067, 1078
NspI RCATGY 2 cut(s) 28, 1307
NspV TTCGAA 2 cut(s) 1111, 2433
PaeI GCATGC 1 cut(s) 1307
PagI TCATGA 2 cut(s) 2239, 2371
PctI GAATGC 2 cut(s) 684, 2200
PflMI CCANNNNNTGG 1 cut(s) 1621
PfoI TCCNGGA 1 cut(s) 1087
PkrI GCNGC 4 cut(s) 1315, 1609, 1657, 1901
PleI GAGTC 4 cut(s) 221, 892, 1376, 1450
PpsI GAGTC 4 cut(s) 221, 892, 1376, 1450
Psp124BI GAGCTC 3 cut(s) 221, 1096, 2540
Psp6I CCWGG 3 cut(s) 995, 1087, 1716
PspGI CCWGG 3 cut(s) 995, 1087, 1716
PspN4I GGNNCC 1 cut(s) 1500
PspPI GGNCC 1 cut(s) 1842
PstNI CAGNNNCTG 1 cut(s) 1157
PsuI RGATCY 4 cut(s) 896, 1498, 2566, 2599
PvuII CAGCTG 2 cut(s) 344, 1541
RsaI GTAC 6 cut(s) 181, 933, 1263, 1297, 1712, 2213
RsaNI GTAC 6 cut(s) 180, 932, 1262, 1296, 1711, 2212
RseI CAYNNNNRTG 2 cut(s) 533, 2481
SacI GAGCTC 3 cut(s) 221, 1096, 2540
SaqAI TTAA 7 cut(s) 869, 957, 1857, 1938, 2055, 2187, 2366
SatI GCNGC 4 cut(s) 1314, 1608, 1656, 1900
Sau96I GGNCC 1 cut(s) 1842
ScaI AGTACT 1 cut(s) 933
SchI GAGTC 4 cut(s) 221, 893, 1376, 1451
ScrFI CCNGG 3 cut(s) 997, 1089, 1718
SduI GDGCHC 4 cut(s) 221, 1096, 1222, 2540
SfaNI GCATC 6 cut(s) 574, 658, 932, 1163, 1909, 2326
SfcI CTRYAG 3 cut(s) 345, 804, 1308
SfuI TTCGAA 2 cut(s) 1111, 2433
SinI GGWCC 1 cut(s) 1842
SmiMI CAYNNNNRTG 2 cut(s) 533, 2481
SmlI CTYRAG 3 cut(s) 1221, 1353, 2522
SmoI CTYRAG 3 cut(s) 1221, 1353, 2522
SphI GCATGC 1 cut(s) 1307
SsiI CCGC 8 cut(s) 309, 328, 625, 823, 1322, 1608, 2596, 2620
SspI AATATT 1 cut(s) 1101
SstI GAGCTC 3 cut(s) 221, 1096, 2540
StyD4I CCNGG 3 cut(s) 995, 1087, 1716
StyI CCWWGG 3 cut(s) 548, 2133, 2307
TaaI ACNGT 6 cut(s) 287, 422, 808, 1199, 1295, 1403
TaiI ACGT 4 cut(s) 907, 1026, 2213, 2385
TaqI TCGA 8 cut(s) 192, 658, 792, 978, 1111, 1928, 2094, 2433
TatI WGTACW 2 cut(s) 179, 931
TauI GCSGC 1 cut(s) 1610
Tru1I TTAA 7 cut(s) 869, 957, 1857, 1938, 2055, 2187, 2366
Tru9I TTAA 7 cut(s) 869, 957, 1857, 1938, 2055, 2187, 2366
TscAI CASTG 6 cut(s) 427, 540, 695, 1087, 1455, 2534
TseFI GTSAC 3 cut(s) 422, 1067, 1078
TseI GCWGC 3 cut(s) 1313, 1655, 1899
Tsp45I GTSAC 3 cut(s) 422, 1067, 1078
TspDTI ATGAA 8 cut(s) 468, 606, 1763, 2091, 2364, 2388, 2558, 2667
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 6 cut(s) 427, 540, 695, 1087, 1455, 2534
Van91I CCANNNNNTGG 1 cut(s) 1621
VpaK11BI GGWCC 1 cut(s) 1842
XapI RAATTY 5 cut(s) 337, 871, 1251, 1882, 2171
XbaI TCTAGA 1 cut(s) 554
XceI RCATGY 2 cut(s) 28, 1307
XcmI CCANNNNNNNNNTGG 1 cut(s) 1618
XmiI GTMKAC 2 cut(s) 810, 1026
ZrmI AGTACT 1 cut(s) 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.