Rroxscaffold_6G00391780

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007066
Physical Location & Seq
Reverse (-)
10604958 .. 10609090
4133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00391780.1

Sequence Viewer

Length: 2487 bp
ATGGAGAGTCAAATTTGGGACCTGTACTTGTTGGTGCTGATAAAAATTTATCATGTTCCTCAGTTTGCCAACTATTTTGGCCTCAAACCACCTTGTCCCTGGTGTTCTTCTAGAGTTGAACACATGTTGGAACCACAAAAGTCCTACTCAGATCGTGTATGTGAGATCCATGCCAATGAGGTATCTAAACTGGGTTACTGTCTCAATCATAAAAGACTGGCTGAATCACATAGTATGTGTAGGAACTGCTTTTCTTCTCAACCAAATTGCAGAGAAAATTCTACTAAAAGTAGGATGGATTTGAGTTCATTGTTGAGTGAGAAATATCAGAAGAAACCTTCCATCTGTTCTTGCTGCAGTAAGAGCTTCAACACCAAACTCTCTCCTCTTTCATTGGAGCATGCTCAGAAAGGAGATTCAAATGTAGAAGCAGTGCATGATGACAACAATCAAGGCACAACCAAACCTGATAGCATGTCAGTCCACAGCGAAAATGGCTATGAGATGGAGACAACTTCTAGCTTAAGAGACACTACTGCAGAAGAACACTGTCGAAATGAAAGTAAGAAACTTGTCCATCAATCTTCCAATGCTACTACCATTGACCTGATTCCTACTGATTTGATTGATTTTTCAACTACAATAGACAGAGGACTCATAAGCAGCTTAAAAGATCAGGAACATCATGATCATGAACAAGGAAGTTTTGATTCCAAGTCAAAGATTGAATTGGAAGATCAACTCTCACGGGAAGCAGCATTACTCATGGTAAATAAGAGTGGAGCAAATACAAGTAATGGAGGACTTACGAGCTTGGAGATGGCCAAGGGATTTACTGATGCTTCATCTATTGAACAAGTGGAAAAGCGAAATCTAGAGTTGGTTGGCAAGCTATGTGATGATTTGGTCACTGCTCCAGAAGCTCAAACTTCATTCGTTGACACTGAACAACTAACAGATACCAAAGAACCTAACGATCCACCAGAATGTGAAGAAGAGAGAATTTTGGCTGTCATTTCAAGAAATCTGTCAAACACAAAAGGGTCACACCATGCAGCGGATCAGCCTCAAGCTCACGAAGATTCTTCACTGCTATTCCTTGAAATATCCAATGCCCCAGCTCACAGTGATGTCGGTACAGGGCCTACAGGAAAATCAATGTTGCAAGACAAAACAAGTATAACTAAAAATGCTCAAGAAGTCAACCATTTTTGCATGTGTTCAGAGCCTAGTGAACCTGAGGAAGAGAGGTTGGTATGCGGACAAAATTGTATAAACACTATCCATTACTTGTACAAAAAGCCGAATGAAAATGGAGACTGTGGGGCAGATGATGGAAGTGTGGCAAGTGTGACGAATGGTGGAGAACCTGTAACAACCATTGAAGAGCTCAAAGCAGCATTGAAGGCTGAAAGTAAAGCTTTAAGTGCTTTATATTCAGAACTAGAGGAAGAGAGAAGTGCGTCTGCAGTAGCTGCCAACCAGACTATGGCTATGATAACAAGGCTTCAAGAAGAGAAGGCAGCAATGCAGATGGAGGCATTGCAATATCAGAGAATGATGGAAGAACAATCTGAGTATGATCAGGAAGCCTTGCAACTTTTGAATGAGTTGATGAACAAAATGGAGAGGGATAAACAAGAGCTAGAGAAAGAATTGGAAATATATCAGAAGAAAGTCCTGGATTATGAGGAAAAAGAGAAGATAAGGGTCATGAGCAAAATCATATATGGAAGTATGAGAAGTAGAAATTCTTCTGCTTCCTGCAGCCAATCTTGGGATAGTGATGCGTTATCCATTGATCTCAATTGTGAAGCAAGGGATGAAGATAGTTCTTTCCCTCAAGAATGCAGTAACAACACCAATAATCCAGATGATGCAGAAGCTCTAAGCTTGGAAGAAATGGCTCTGGATTGTGTTAAGCAGATGAGCATTCTTGATGACTCATTGGCAGGCTTCCAGGAAGAGAGGTTGTCCGTTCTGGATCAGCTTAAAGCATTGGAAGATAGGCTAGTAGTGTTGAGTGAAAATGAGAACTACTCTCAGGATGCTAATTTAGTTGAGAATTCCTCGAACTACAGTACCAAAAACATTGAAGAAAATCATGATTTCAGCAGTCCAGAAGAAAAACAAATATCAGATGAGAAAATAACATTGGCTTCCATGGCAAAGAGGCTTCTTCCACTTTTGGGTGTAGCAGCTGAGGATAATGAAACTGAAGCAGGTTTAACAAATGAAAAACAAGTCAGAACTGAATCTATTGCAATGCATAACTCGGTGACCAATTCTGAATTGCATGATACCAGAATAGCCATTGAGGAGGAGGTGGATCATGTTTATGAGAGGCTACAAGCTCTTGAAAAATGTAATTTGGAGAAGGGGATGGATCTTCTTCAAGAGAGCTTGCAACATCTCAAAGATTTGAGGGATGTTGAACTCCGCATAAGAAACATTAATGTTCCTGTGGATGAAGCTGCAGGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008092 GO:0017022
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

828

Amino Acids

92.72

Weight (kDa)

4.68

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 463 - 553 1.3e-30 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 2213, 2468
AccB7I CCANNNNNTGG 1 cut(s) 1489
AciI CCGC 3 cut(s) 1058, 1260, 2440
AclWI GGATC 6 cut(s) 160, 971, 1068, 1992, 2337, 2394
AcoI YGGCCR 1 cut(s) 822
AcsI RAATTY 6 cut(s) 12, 45, 277, 1002, 1750, 2065
AcuI CTGAAG 1 cut(s) 2238
AfaI GTAC 4 cut(s) 26, 1138, 1295, 2083
AfiI CCNNNNNNNGG 4 cut(s) 1057, 1489, 1777, 2189
AflII CTTAAG 1 cut(s) 523
AflIII ACRYGT 1 cut(s) 123
AjnI CCWGG 3 cut(s) 98, 1680, 1959
AjuI GAANNNNNNNTTGG 2 cut(s) 2138, 2170
Alw21I GWGCWC 1 cut(s) 1392
Alw26I GTCTC 4 cut(s) 206, 503, 522, 1311
AlwI GGATC 6 cut(s) 160, 971, 1068, 1992, 2337, 2394
AlwNI CAGNNNCTG 1 cut(s) 1475
AoxI GGCC 3 cut(s) 79, 822, 1142
ApoI RAATTY 6 cut(s) 12, 45, 277, 1002, 1750, 2065
AseI ATTAAT 1 cut(s) 2454
Asp700I GAANNNNTTC 1 cut(s) 1753
AspS9I GGNCC 2 cut(s) 19, 1142
AsuHPI GGTGA 1 cut(s) 2290
AvaII GGWCC 1 cut(s) 19
AxyI CCTNAGG 1 cut(s) 1239
BaeI ACNNNNGTAYC 2 cut(s) 2065, 2098
BalI TGGCCA 1 cut(s) 824
BanII GRGCYC 1 cut(s) 1392
BarI GAAGNNNNNNTAC 4 cut(s) 744, 776, 1738, 1770
Bbv12I GWGCWC 1 cut(s) 1392
BbvCI CCTCAGC 1 cut(s) 2202
BccI CCATC 9 cut(s) 289, 350, 499, 585, 814, 1328, 1528, 1555, 2377
BciT130I CCWGG 3 cut(s) 100, 1682, 1961
BclI TGATCA 2 cut(s) 688, 1582
BcoDI GTCTC 4 cut(s) 206, 503, 522, 1311
BfaI CTAG 7 cut(s) 111, 519, 875, 1230, 1445, 1646, 2012
BfmI CTRYAG 7 cut(s) 355, 537, 1146, 1467, 1765, 2077, 2475
BfrI CTTAAG 1 cut(s) 523
BfuAI ACCTGC 2 cut(s) 2213, 2468
Bme1390I CCNGG 3 cut(s) 100, 1682, 1961
Bme18I GGWCC 1 cut(s) 19
BmgT120I GGNCC 2 cut(s) 19, 1142
BmiI GGNNCC 2 cut(s) 20, 132
BmrFI CCNGG 3 cut(s) 100, 1682, 1961
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 4 cut(s) 829, 1777, 1867, 2038
BmuI ACTGGG 1 cut(s) 200
BplI GAGNNNNNCTC 4 cut(s) 2024, 2056, 2054, 2086
BpmI CTGGAG 1 cut(s) 900
Bpu10I CCTNAGC 1 cut(s) 2202
BpuEI CTTGAG 3 cut(s) 1053, 1179, 1827
BsaJI CCNNGG 3 cut(s) 98, 825, 2163
BsaXI ACNNNNNCTCC 2 cut(s) 1356, 1386
Bsc4I CCNNNNNNNGG 4 cut(s) 1057, 1489, 1777, 2189
Bse1I ACTGG 2 cut(s) 195, 222
Bse21I CCTNAGG 1 cut(s) 1239
Bse3DI GCAATG 3 cut(s) 1533, 1541, 2271
BseBI CCWGG 3 cut(s) 100, 1682, 1961
BseDI CCNNGG 3 cut(s) 98, 825, 2163
BseGI GGATG 6 cut(s) 300, 1828, 2053, 2388, 2434, 2473
BseLI CCNNNNNNNGG 4 cut(s) 1057, 1489, 1777, 2189
BseMI GCAATG 3 cut(s) 1533, 1541, 2271
BseMII CTCAG 7 cut(s) 74, 162, 419, 1230, 1566, 2057, 2193
BseNI ACTGG 2 cut(s) 195, 222
BseRI GAGGAG 3 cut(s) 375, 2333, 2336
BseYI CCCAGC 1 cut(s) 1117
BshFI GGCC 3 cut(s) 81, 824, 1144
BsiHKAI GWGCWC 1 cut(s) 1392
BslFI GGGAC 2 cut(s) 32, 81
BslI CCNNNNNNNGG 4 cut(s) 1057, 1489, 1777, 2189
BsmAI GTCTC 4 cut(s) 206, 503, 522, 1311
BsmFI GGGAC 2 cut(s) 32, 81
BsmI GAATGC 2 cut(s) 1853, 1932
BsnI GGCC 3 cut(s) 81, 824, 1144
Bsp1286I GDGCHC 1 cut(s) 1392
Bsp1407I TGTACA 1 cut(s) 1293
Bsp19I CCATGG 1 cut(s) 2163
BspACI CCGC 3 cut(s) 1058, 1260, 2440
BspANI GGCC 3 cut(s) 81, 824, 1144
BspCNI CTCAG 7 cut(s) 73, 161, 418, 1231, 1567, 2056, 2194
BspHI TCATGA 4 cut(s) 685, 691, 1713, 2104
BspLI GGNNCC 2 cut(s) 20, 132
BspMAI CTGCAG 5 cut(s) 359, 541, 1471, 1769, 2479
BspMI ACCTGC 2 cut(s) 2213, 2468
BspPI GGATC 6 cut(s) 160, 971, 1068, 1992, 2337, 2394
BspQI GCTCTTC 1 cut(s) 1380
BspTI CTTAAG 1 cut(s) 523
BsrDI GCAATG 3 cut(s) 1533, 1541, 2271
BsrGI TGTACA 1 cut(s) 1293
BsrI ACTGG 2 cut(s) 195, 222
BssECI CCNNGG 3 cut(s) 98, 825, 2163
BssT1I CCWWGG 2 cut(s) 825, 2163
Bst2UI CCWGG 3 cut(s) 100, 1682, 1961
Bst4CI ACNGT 5 cut(s) 200, 551, 1127, 1322, 2081
Bst6I CTCTTC 6 cut(s) 990, 1239, 1380, 1446, 1509, 1959
BstAFI CTTAAG 1 cut(s) 523
BstAPI GCANNNNNTGC 1 cut(s) 1475
BstAUI TGTACA 1 cut(s) 1293
BstC8I GCNNGC 4 cut(s) 402, 890, 1954, 2405
BstDEI CTNAG 8 cut(s) 60, 148, 405, 1239, 1575, 1889, 2043, 2202
BstDSI CCRYGG 1 cut(s) 2163
BstEII GGTNACC 1 cut(s) 2278
BstF5I GGATG 6 cut(s) 300, 1828, 2053, 2388, 2434, 2473
BstMAI GTCTC 4 cut(s) 206, 503, 522, 1311
BstMWI GCNNNNNNNGC 7 cut(s) 363, 495, 920, 1406, 1427, 1475, 2165
BstNI CCWGG 3 cut(s) 100, 1682, 1961
BstNSI RCATGY 4 cut(s) 127, 404, 478, 1219
BstPI GGTNACC 1 cut(s) 2278
BstSCI CCNGG 3 cut(s) 98, 1680, 1959
BstSFI CTRYAG 7 cut(s) 355, 537, 1146, 1467, 1765, 2077, 2475
BstX2I RGATCY 2 cut(s) 165, 2386
BstYI RGATCY 2 cut(s) 165, 2386
Bsu36I CCTNAGG 1 cut(s) 1239
BsuRI GGCC 3 cut(s) 81, 824, 1144
BtgI CCRYGG 1 cut(s) 2163
BtsCI GGATG 6 cut(s) 300, 1828, 2053, 2388, 2434, 2473
BtsI GCAGTG 3 cut(s) 438, 909, 1088
BtsIMutI CAGTG 6 cut(s) 438, 547, 909, 942, 1088, 1132
BveI ACCTGC 2 cut(s) 2213, 2468
Cac8I GCNNGC 4 cut(s) 402, 890, 1954, 2405
CaiI CAGNNNCTG 1 cut(s) 1475
CciI TCATGA 4 cut(s) 685, 691, 1713, 2104
Cfr13I GGNCC 2 cut(s) 19, 1142
CseI GACGC 1 cut(s) 1452
Csp6I GTAC 4 cut(s) 25, 1137, 1294, 2082
CviQI GTAC 4 cut(s) 25, 1137, 1294, 2082
DdeI CTNAG 8 cut(s) 60, 148, 405, 1239, 1575, 1889, 2043, 2202
EaeI YGGCCR 1 cut(s) 822
Eam1104I CTCTTC 6 cut(s) 990, 1239, 1380, 1446, 1509, 1959
EarI CTCTTC 6 cut(s) 990, 1239, 1380, 1446, 1509, 1959
Ecl136II GAGCTC 1 cut(s) 1390
Eco130I CCWWGG 2 cut(s) 825, 2163
Eco24I GRGCYC 1 cut(s) 1392
Eco47I GGWCC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 1390
Eco57I CTGAAG 1 cut(s) 2238
Eco81I CCTNAGG 1 cut(s) 1239
Eco91I GGTNACC 1 cut(s) 2278
EcoICRI GAGCTC 1 cut(s) 1390
EcoO109I RGGNCCY 2 cut(s) 19, 1142
EcoO65I GGTNACC 1 cut(s) 2278
EcoRI GAATTC 1 cut(s) 2065
EcoRII CCWGG 3 cut(s) 98, 1680, 1959
EcoT14I CCWWGG 2 cut(s) 825, 2163
EcoT22I ATGCAT 1 cut(s) 2271
EcoT38I GRGCYC 1 cut(s) 1392
ErhI CCWWGG 2 cut(s) 825, 2163
FalI AAGNNNNNCTT 2 cut(s) 1405, 1437
FaqI GGGAC 2 cut(s) 32, 81
FbaI TGATCA 2 cut(s) 688, 1582
FokI GGATG 6 cut(s) 307, 1835, 2060, 2395, 2441, 2480
FriOI GRGCYC 1 cut(s) 1392
FspBI CTAG 7 cut(s) 111, 519, 875, 1230, 1445, 1646, 2012
GsaI CCCAGC 1 cut(s) 1121
GsuI CTGGAG 1 cut(s) 900
HaeIII GGCC 3 cut(s) 81, 824, 1144
HgaI GACGC 1 cut(s) 1452
HincII GTYRAC 2 cut(s) 940, 1204
HindII GTYRAC 2 cut(s) 940, 1204
HindIII AAGCTT 2 cut(s) 1419, 1891
HinfI GANTC 9 cut(s) 7, 224, 416, 610, 654, 710, 1082, 1943, 2255
HphI GGTGA 1 cut(s) 2290
Hpy166II GTNNAC 4 cut(s) 484, 940, 1204, 1235
Hpy8I GTNNAC 4 cut(s) 484, 940, 1204, 1235
HpyAV CCTTC 4 cut(s) 348, 1399, 1513, 2371
HpyCH4III ACNGT 5 cut(s) 200, 551, 1127, 1322, 2081
HpyF10VI GCNNNNNNNGC 7 cut(s) 363, 495, 920, 1406, 1427, 1475, 2165
HpyF3I CTNAG 8 cut(s) 60, 148, 405, 1239, 1575, 1889, 2043, 2202
Ksp22I TGATCA 2 cut(s) 688, 1582
LguI GCTCTTC 1 cut(s) 1380
LmnI GCTCC 3 cut(s) 397, 782, 919
LweI GCATC 4 cut(s) 829, 1777, 1867, 2038
MaeI CTAG 7 cut(s) 111, 519, 875, 1230, 1445, 1646, 2012
MaeIII GTNAC 7 cut(s) 194, 907, 1044, 1351, 1372, 1853, 2278
MfeI CAATTG 1 cut(s) 1807
MflI RGATCY 2 cut(s) 165, 2386
MhlI GDGCHC 1 cut(s) 1392
MlsI TGGCCA 1 cut(s) 824
MluNI TGGCCA 1 cut(s) 824
MlyI GAGTC 3 cut(s) 16, 648, 1937
MmeI TCCRAC 1 cut(s) 108
Mox20I TGGCCA 1 cut(s) 824
Mph1103I ATGCAT 1 cut(s) 2271
MroXI GAANNNNTTC 1 cut(s) 1753
MscI TGGCCA 1 cut(s) 824
MseI TTAA 7 cut(s) 524, 668, 1424, 1920, 1992, 2228, 2454
MslI CAYNNNNRTG 5 cut(s) 174, 690, 985, 1128, 2337
Msp20I TGGCCA 1 cut(s) 824
MspA1I CMGCKG 2 cut(s) 1058, 2201
MspCI CTTAAG 1 cut(s) 523
MspR9I CCNGG 3 cut(s) 100, 1682, 1961
MunI CAATTG 1 cut(s) 1807
Mva1269I GAATGC 2 cut(s) 1853, 1932
MvaI CCWGG 3 cut(s) 100, 1682, 1961
MwoI GCNNNNNNNGC 7 cut(s) 363, 495, 920, 1406, 1427, 1475, 2165
NcoI CCATGG 1 cut(s) 2163
NlaIV GGNNCC 2 cut(s) 20, 132
NmuCI GTSAC 4 cut(s) 907, 1044, 1351, 2278
NsiI ATGCAT 1 cut(s) 2271
NspI RCATGY 4 cut(s) 127, 404, 478, 1219
PaeI GCATGC 1 cut(s) 404
PagI TCATGA 4 cut(s) 685, 691, 1713, 2104
PciI ACATGT 1 cut(s) 123
PciSI GCTCTTC 1 cut(s) 1380
PctI GAATGC 2 cut(s) 1853, 1932
PdmI GAANNNNTTC 1 cut(s) 1753
PfeI GAWTC 6 cut(s) 224, 416, 610, 710, 1082, 2255
PflMI CCANNNNNTGG 1 cut(s) 1489
PfoI TCCNGGA 2 cut(s) 1680, 1959
PleI GAGTC 3 cut(s) 15, 648, 1937
PpsI GAGTC 3 cut(s) 15, 648, 1937
PpuMI RGGWCCY 1 cut(s) 19
PscI ACATGT 1 cut(s) 123
PshBI ATTAAT 1 cut(s) 2454
Psp124BI GAGCTC 1 cut(s) 1392
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 3 cut(s) 98, 1680, 1959
PspEI GGTNACC 1 cut(s) 2278
PspFI CCCAGC 1 cut(s) 1117
PspGI CCWGG 3 cut(s) 98, 1680, 1959
PspN4I GGNNCC 2 cut(s) 20, 132
PspPI GGNCC 2 cut(s) 19, 1142
PspPPI RGGWCCY 1 cut(s) 19
PstI CTGCAG 5 cut(s) 359, 541, 1471, 1769, 2479
PstNI CAGNNNCTG 1 cut(s) 1475
PsuI RGATCY 2 cut(s) 165, 2386
PvuII CAGCTG 1 cut(s) 2201
RsaI GTAC 4 cut(s) 26, 1138, 1295, 2083
RsaNI GTAC 4 cut(s) 25, 1137, 1294, 2082
RseI CAYNNNNRTG 5 cut(s) 174, 690, 985, 1128, 2337
SacI GAGCTC 1 cut(s) 1392
SapI GCTCTTC 1 cut(s) 1380
SaqAI TTAA 7 cut(s) 524, 668, 1424, 1920, 1992, 2228, 2454
Sau96I GGNCC 2 cut(s) 19, 1142
SchI GAGTC 3 cut(s) 16, 648, 1937
ScrFI CCNGG 3 cut(s) 100, 1682, 1961
SduI GDGCHC 1 cut(s) 1392
SfaNI GCATC 4 cut(s) 829, 1777, 1867, 2038
SfcI CTRYAG 7 cut(s) 355, 537, 1146, 1467, 1765, 2077, 2475
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 5 cut(s) 174, 690, 985, 1128, 2337
SmlI CTYRAG 4 cut(s) 523, 1068, 1194, 1842
SmoI CTYRAG 4 cut(s) 523, 1068, 1194, 1842
SphI GCATGC 1 cut(s) 404
SsiI CCGC 3 cut(s) 1058, 1260, 2440
SspMI CTAG 7 cut(s) 111, 519, 875, 1230, 1445, 1646, 2012
SstI GAGCTC 1 cut(s) 1392
StyD4I CCNGG 3 cut(s) 98, 1680, 1959
StyI CCWWGG 2 cut(s) 825, 2163
TaaI ACNGT 5 cut(s) 200, 551, 1127, 1322, 2081
TaqI TCGA 2 cut(s) 553, 2072
TatI WGTACW 2 cut(s) 24, 1293
TfiI GAWTC 6 cut(s) 224, 416, 610, 710, 1082, 2255
Tru1I TTAA 7 cut(s) 524, 668, 1424, 1920, 1992, 2228, 2454
Tru9I TTAA 7 cut(s) 524, 668, 1424, 1920, 1992, 2228, 2454
TscAI CASTG 6 cut(s) 438, 554, 916, 949, 1095, 1132
TseFI GTSAC 4 cut(s) 907, 1044, 1351, 2278
Tsp45I GTSAC 4 cut(s) 907, 1044, 1351, 2278
TspGWI ACGGA 1 cut(s) 1966
TspRI CASTG 6 cut(s) 438, 554, 916, 949, 1095, 1132
Van91I CCANNNNNTGG 1 cut(s) 1489
Vha464I CTTAAG 1 cut(s) 523
VpaK11BI GGWCC 1 cut(s) 19
VspI ATTAAT 1 cut(s) 2454
XapI RAATTY 6 cut(s) 12, 45, 277, 1002, 1750, 2065
XbaI TCTAGA 2 cut(s) 110, 874
XceI RCATGY 4 cut(s) 127, 404, 478, 1219
XcmI CCANNNNNNNNNTGG 3 cut(s) 96, 491, 1486
XmnI GAANNNNTTC 1 cut(s) 1753
XspI CTAG 7 cut(s) 111, 519, 875, 1230, 1445, 1646, 2012
Zsp2I ATGCAT 1 cut(s) 2271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.