AT5G18400

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S cluster from NADPH via

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008748
Physical Location & Seq
Reverse (-)
6092682 .. 6095080
2399 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G18400.1

Sequence Viewer

Length: 810 bp
ATGATGAATCAGAAGACTGTTTTGGCTGTGACGGATGATGTGGTGTTACCTGTTAGCTCTGTTTTAGCTATAATGAAAGAGCTTGGAAAGGAAGTGATTGAGAGTTTTGATCCTCTGATTATTACTCAAGCATCCACAATAAATCAGTTTCCTTTGGATGCTTCCTCTGTGGAGGCGGTATTAGCCATTTCCAAAACATCTGATTTCCCAAGTGATAAAATATGTGGTGAGTTCTCAAGAATCTTGAAGCCTGGCGGCACGGTTTCTGTGTGCAAAGTTTTGGAGGGTGAAACGGGAGAAATTCAACAGACCATTCAAAGGAGAGTGACATTGGCTGGTTTTCTTGAGCCACAGTGTCTTGATTTGAAATCAATTAAATTGTCTACGTTCAGTTTATCCTTTGGGATTAAGGCAAAGAAACCTTCTTGGAAGATTGGTTCATCATTTGCTCTCAAAAAGCCTGTAACAAATCTCTTTAAGATTGATCTAGATGACGACGTGGATCTTATTGATGAAGATTCTCTTTTAACAGAGGAGGACTTGATGAAGCCACAGCTTCCTGTTGCCAGCGGTTGTGAGACCACTAAAAAAGCTTGCAAGAACTGCGTCTGTGGTAGAGCTGAAATTGAGGAGAAAGCTGTGAAGCTGGGCCTCACAGAGGATCAAATCGAGAATCCACAATCATCATGCGGCAGCTGTGGGCTCGGTGATGCCTTCCGCTGCGGTACATGCCCTTACAAGGGTCTGCCACCCTTCAAATTAGGCGAGAAGGTAACCCTATCTCAAAACTTCCTTGAAGCTGACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006790 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016226 GO:0022607 GO:0031163 GO:0044085 GO:0044237 GO:0044424 GO:0044464 GO:0051186 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIAPIN1 Methyltransf_11

Protein Analysis

269

Amino Acids

29.1

Weight (kDa)

4.86

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CIAPIN1 PF05093 221 - 260 2.2e-15 Cytokine-induced anti-apoptosis inhibitor 1, Fe-S biogenesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 383
AciI CCGC 6 cut(s) 176, 255, 570, 690, 718, 723
AclWI GGATC 3 cut(s) 104, 510, 669
AcsI RAATTY 1 cut(s) 300
AfaI GTAC 1 cut(s) 727
AfiI CCNNNNNNNGG 4 cut(s) 318, 658, 739, 740
AgsI TTSAA 6 cut(s) 247, 305, 317, 367, 757, 797
AjiI CACGTC 1 cut(s) 499
AjnI CCWGG 1 cut(s) 250
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AloI GAACNNNNNNTCC 2 cut(s) 421, 453
Alw26I GTCTC 1 cut(s) 572
AlwI GGATC 3 cut(s) 104, 510, 669
AoxI GGCC 1 cut(s) 649
ApeKI GCWGC 2 cut(s) 693, 720
ApoI RAATTY 1 cut(s) 300
AspS9I GGNCC 1 cut(s) 649
AsuHPI GGTGA 3 cut(s) 239, 299, 719
BanII GRGCYC 1 cut(s) 705
BbsI GAAGAC 1 cut(s) 20
BbvI GCAGC 2 cut(s) 705, 707
BciT130I CCWGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 572
BfaI CTAG 1 cut(s) 488
BisI GCNGC 4 cut(s) 256, 691, 694, 721
BlsI GCNGC 4 cut(s) 257, 692, 695, 722
Bme1390I CCNGG 1 cut(s) 252
BmgBI CACGTC 1 cut(s) 499
BmgT120I GGNCC 1 cut(s) 649
BmrFI CCNGG 1 cut(s) 252
BmsI GCATC 3 cut(s) 140, 148, 700
BpiI GAAGAC 1 cut(s) 20
BpuEI CTTGAG 3 cut(s) 111, 220, 365
BsaI GGTCTC 1 cut(s) 572
Bsc4I CCNNNNNNNGG 4 cut(s) 318, 658, 739, 740
BseBI CCWGG 1 cut(s) 252
BseGI GGATG 3 cut(s) 40, 131, 163
BseLI CCNNNNNNNGG 4 cut(s) 318, 658, 739, 740
BseRI GAGGAG 2 cut(s) 548, 644
BseXI GCAGC 2 cut(s) 705, 707
BseYI CCCAGC 1 cut(s) 646
BshFI GGCC 1 cut(s) 651
BslI CCNNNNNNNGG 4 cut(s) 318, 658, 739, 740
BsmAI GTCTC 1 cut(s) 572
BsnI GGCC 1 cut(s) 651
Bso31I GGTCTC 1 cut(s) 572
Bsp1286I GDGCHC 1 cut(s) 705
Bsp143I GATC 4 cut(s) 109, 484, 502, 661
BspACI CCGC 6 cut(s) 176, 255, 570, 690, 718, 723
BspANI GGCC 1 cut(s) 651
BspPI GGATC 3 cut(s) 104, 510, 669
BspTNI GGTCTC 1 cut(s) 572
BssMI GATC 4 cut(s) 109, 484, 502, 661
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 3 cut(s) 19, 262, 354
BstAPI GCANNNNNTGC 1 cut(s) 603
BstC8I GCNNGC 2 cut(s) 568, 595
BstEII GGTNACC 1 cut(s) 772
BstENI CCTNNNNNAGG 1 cut(s) 656
BstF5I GGATG 3 cut(s) 40, 131, 163
BstKTI GATC 4 cut(s) 112, 487, 505, 664
BstMAI GTCTC 1 cut(s) 572
BstMBI GATC 4 cut(s) 109, 484, 502, 661
BstMWI GCNNNNNNNGC 3 cut(s) 182, 603, 729
BstNI CCWGG 1 cut(s) 252
BstNSI RCATGY 1 cut(s) 732
BstPI GGTNACC 1 cut(s) 772
BstSCI CCNGG 1 cut(s) 250
BstV1I GCAGC 2 cut(s) 705, 707
BstV2I GAAGAC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
BsuRI GGCC 1 cut(s) 651
BtrI CACGTC 1 cut(s) 499
BtsCI GGATG 3 cut(s) 40, 131, 163
BtsIMutI CAGTG 1 cut(s) 359
Cac8I GCNNGC 2 cut(s) 568, 595
Cfr13I GGNCC 1 cut(s) 649
CseI GACGC 1 cut(s) 595
Csp6I GTAC 1 cut(s) 726
CviAII CATG 2 cut(s) 687, 729
CviQI GTAC 1 cut(s) 726
DpnI GATC 4 cut(s) 111, 486, 504, 663
DpnII GATC 4 cut(s) 109, 484, 502, 661
Eco24I GRGCYC 1 cut(s) 705
Eco31I GGTCTC 1 cut(s) 572
Eco91I GGTNACC 1 cut(s) 772
EcoNI CCTNNNNNAGG 1 cut(s) 656
EcoO65I GGTNACC 1 cut(s) 772
EcoRII CCWGG 1 cut(s) 250
EcoT38I GRGCYC 1 cut(s) 705
FaeI CATG 2 cut(s) 690, 732
FaiI YATR 6 cut(s) 71, 223, 688, 730, 806, 808
FalI AAGNNNNNCTT 2 cut(s) 507, 539
FatI CATG 2 cut(s) 686, 728
FblI GTMKAC 1 cut(s) 383
Fnu4HI GCNGC 4 cut(s) 256, 691, 694, 721
FokI GGATG 3 cut(s) 47, 118, 170
FriOI GRGCYC 1 cut(s) 705
Fsp4HI GCNGC 4 cut(s) 256, 691, 694, 721
FspBI CTAG 1 cut(s) 488
GluI GCNGC 4 cut(s) 256, 691, 694, 721
GsaI CCCAGC 1 cut(s) 650
HaeIII GGCC 1 cut(s) 651
HgaI GACGC 1 cut(s) 595
Hin1II CATG 2 cut(s) 690, 732
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 4 cut(s) 7, 240, 518, 673
HphI GGTGA 3 cut(s) 239, 299, 719
Hpy166II GTNNAC 1 cut(s) 384
Hpy188I TCNGA 3 cut(s) 12, 117, 202
Hpy188III TCNNGA 6 cut(s) 237, 244, 344, 359, 488, 670
Hpy8I GTNNAC 1 cut(s) 384
Hpy99I CGWCG 1 cut(s) 500
HpyAV CCTTC 4 cut(s) 432, 724, 763, 763
HpyCH4III ACNGT 3 cut(s) 19, 262, 354
HpyCH4IV ACGT 2 cut(s) 386, 498
HpyCH4V TGCA 2 cut(s) 273, 597
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 603, 729
HpySE526I ACGT 2 cut(s) 386, 498
Hsp92II CATG 2 cut(s) 690, 732
Kzo9I GATC 4 cut(s) 109, 484, 502, 661
LpnPI CCDG 8 cut(s) 63, 237, 264, 321, 474, 573, 580, 632
Lsp1109I GCAGC 2 cut(s) 705, 707
LweI GCATC 3 cut(s) 140, 148, 700
MaeI CTAG 1 cut(s) 488
MaeII ACGT 2 cut(s) 386, 498
MaeIII GTNAC 5 cut(s) 28, 45, 325, 463, 772
MalI GATC 4 cut(s) 111, 486, 504, 663
MboI GATC 4 cut(s) 109, 484, 502, 661
MboII GAAGA 3 cut(s) 25, 442, 527
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 1 cut(s) 705
MluCI AATT 5 cut(s) 300, 372, 377, 624, 758
MnlI CCTC 9 cut(s) 123, 166, 175, 277, 526, 529, 622, 652, 662
MseI TTAA 4 cut(s) 375, 408, 477, 527
MspA1I CMGCKG 3 cut(s) 570, 696, 720
MspR9I CCNGG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 3 cut(s) 182, 603, 729
NdeII GATC 4 cut(s) 109, 484, 502, 661
NlaIII CATG 2 cut(s) 690, 732
NmuCI GTSAC 2 cut(s) 28, 325
NspI RCATGY 1 cut(s) 732
PfeI GAWTC 4 cut(s) 7, 240, 518, 673
PkrI GCNGC 4 cut(s) 257, 692, 695, 722
Psp6I CCWGG 1 cut(s) 250
PspEI GGTNACC 1 cut(s) 772
PspFI CCCAGC 1 cut(s) 646
PspGI CCWGG 1 cut(s) 250
PspPI GGNCC 1 cut(s) 649
PsuI RGATCY 1 cut(s) 502
PvuII CAGCTG 1 cut(s) 696
RsaI GTAC 1 cut(s) 727
RsaNI GTAC 1 cut(s) 726
SaqAI TTAA 4 cut(s) 375, 408, 477, 527
SatI GCNGC 4 cut(s) 256, 691, 694, 721
Sau3AI GATC 4 cut(s) 109, 484, 502, 661
Sau96I GGNCC 1 cut(s) 649
ScrFI CCNGG 1 cut(s) 252
SduI GDGCHC 1 cut(s) 705
SfaNI GCATC 3 cut(s) 140, 148, 700
SmlI CTYRAG 3 cut(s) 126, 235, 344
SmoI CTYRAG 3 cut(s) 126, 235, 344
Sse9I AATT 5 cut(s) 300, 372, 377, 624, 758
SsiI CCGC 6 cut(s) 176, 255, 570, 690, 718, 723
SspMI CTAG 1 cut(s) 488
StyD4I CCNGG 1 cut(s) 250
TaaI ACNGT 3 cut(s) 19, 262, 354
TaiI ACGT 2 cut(s) 389, 501
TaqI TCGA 1 cut(s) 669
TasI AATT 5 cut(s) 300, 372, 377, 624, 758
TauI GCSGC 2 cut(s) 258, 693
TfiI GAWTC 4 cut(s) 7, 240, 518, 673
Tru1I TTAA 4 cut(s) 375, 408, 477, 527
Tru9I TTAA 4 cut(s) 375, 408, 477, 527
TscAI CASTG 1 cut(s) 359
TseFI GTSAC 2 cut(s) 28, 325
TseI GCWGC 2 cut(s) 693, 720
Tsp45I GTSAC 2 cut(s) 28, 325
TspDTI ATGAA 5 cut(s) 20, 89, 429, 528, 560
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 1 cut(s) 359
XagI CCTNNNNNAGG 1 cut(s) 656
XapI RAATTY 1 cut(s) 300
XbaI TCTAGA 1 cut(s) 487
XceI RCATGY 1 cut(s) 732
XmiI GTMKAC 1 cut(s) 383
XspI CTAG 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.