Rh4BG442300

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S cluster from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Has anti- apoptotic effects in the cell

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008748
Physical Location & Seq
Forward (+)
58213291 .. 58215537
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG442300.1

Sequence Viewer

Length: 783 bp
ATGGGATCGGTGCTGGCATTTACAGATGATGCGGTTCTACCTGCCAGTCTTGTTTTTGATGCATTAAGGGAGGTTGGGAATGGAGCTTCAGATAAGTGTGAACCTCAAATTATCACTCAAGTGTCTTCCTTGGGTCAGCTGCCACAGGGGACTTCTTCTATCGACATTGTGTTTGCCCTTTGCAGATCAATTGAATTCCTGAGTGAGCAGTTGTTGAAGGAGACCTCAAGAGTCTTGAAGCCTGGTGGAACCATCCTAGTTTACAAGACTTCTGAAACAGATAAGACCACCTCTGTTCTTGAGCGCAAGTTACTTTTGTCTGGTTTCCTAGAAGCACAAGCTCTTCAAACAAAATCATCTGAGTTGTGCTCTGGAGTCAAGGCCAAAAAGCCTTCTTGGAAAATTGGCTCATCCTTTGCCTTAAAAAAGCCTTCAAAAACTCTACCGAAGTTGCAGATCGATGATGATTCAGATCTGATTGATGAGGATAGTCTATTAACAGAAGAGGACTTGAAGAAACCCCAGCCGGTCGTAGCTGATGATTGTGAAGTTGGAAGCACAAGGAAAGCTTGTAAAAATTGCACTTGTGGGAGGGCTGAAGTGGAGCAAAAAGTAGAGAAGTTACAATCCACTGTGGATTTGAGCAGTTTCCAGTCAGCATGTGGCAGTTGTGGACTAGGGGATGCTTTCCGATGCGCTACATGTCCTTATAAAGGTCTTCCTCCATTCAAACCGGGCGAGAAGGTAGCGTTCTCTTCAAACTTCCTTGCAGCAGACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006790 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016226 GO:0022607 GO:0031163 GO:0044085 GO:0044237 GO:0044424 GO:0044464 GO:0051186 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIAPIN1 Methyltransf_11

Protein Analysis

260

Amino Acids

27.87

Weight (kDa)

5.17

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anamorsin_N PF20922 39 - 119 2e-06 Anamorsin, N-terminal
CIAPIN1 PF05093 205 - 250 1.9e-15 Cytokine-induced anti-apoptosis inhibitor 1, Fe-S biogenesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 711
Acc36I ACCTGC 1 cut(s) 49
AciI CCGC 1 cut(s) 32
AclWI GGATC 1 cut(s) 13
AcsI RAATTY 1 cut(s) 194
AcuI CTGAAG 2 cut(s) 72, 618
AfiI CCNNNNNNNGG 1 cut(s) 713
AflIII ACRYGT 1 cut(s) 701
AgsI TTSAA 8 cut(s) 194, 217, 238, 347, 435, 514, 730, 759
AjnI CCWGG 1 cut(s) 241
AleI CACNNNNGTG 1 cut(s) 119
AluBI AGCT 5 cut(s) 86, 139, 341, 536, 569
AluI AGCT 5 cut(s) 86, 139, 341, 536, 569
Alw21I GWGCWC 1 cut(s) 371
Alw26I GTCTC 1 cut(s) 215
AlwI GGATC 1 cut(s) 13
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 2 cut(s) 139, 770
ApoI RAATTY 1 cut(s) 194
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AspLEI GCGC 2 cut(s) 306, 698
AsuC2I CCSGG 1 cut(s) 735
BbsI GAAGAC 2 cut(s) 117, 710
Bbv12I GWGCWC 1 cut(s) 371
BbvI GCAGC 1 cut(s) 126
BccI CCATC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 243
BcnI CCSGG 1 cut(s) 735
BcoDI GTCTC 1 cut(s) 215
BfaI CTAG 3 cut(s) 257, 329, 677
BfuAI ACCTGC 1 cut(s) 49
BglII AGATCT 1 cut(s) 472
BisI GCNGC 2 cut(s) 140, 771
BlsI GCNGC 2 cut(s) 141, 772
Bme1390I CCNGG 2 cut(s) 243, 735
BmiI GGNNCC 1 cut(s) 250
BmrFI CCNGG 2 cut(s) 243, 735
BmsI GCATC 4 cut(s) 19, 49, 673, 683
BpiI GAAGAC 2 cut(s) 117, 710
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpmI CTGGAG 1 cut(s) 393
BpuEI CTTGAG 3 cut(s) 102, 211, 320
BpuMI CCSGG 1 cut(s) 735
Bsa29I ATCGAT 1 cut(s) 459
BsaBI GATNNNNATC 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 129
Bsc4I CCNNNNNNNGG 1 cut(s) 713
Bse118I RCCGGY 1 cut(s) 526
Bse1I ACTGG 2 cut(s) 45, 652
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 1 cut(s) 243
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 3 cut(s) 252, 410, 688
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 1 cut(s) 713
BseMII CTCAG 2 cut(s) 191, 351
BseNI ACTGG 2 cut(s) 45, 652
BseXI GCAGC 1 cut(s) 126
BseYI CCCAGC 1 cut(s) 522
Bsh1285I CGRYCG 1 cut(s) 531
BshFI GGCC 1 cut(s) 383
BshVI ATCGAT 1 cut(s) 459
BsiEI CGRYCG 1 cut(s) 531
BsiHKAI GWGCWC 1 cut(s) 371
BsiSI CCGG 2 cut(s) 527, 734
BslFI GGGAC 1 cut(s) 163
BslI CCNNNNNNNGG 1 cut(s) 713
BsmAI GTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 163
BsnI GGCC 1 cut(s) 383
Bso31I GGTCTC 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 371
Bsp143I GATC 4 cut(s) 5, 185, 456, 472
BspACI CCGC 1 cut(s) 32
BspANI GGCC 1 cut(s) 383
BspCNI CTCAG 2 cut(s) 192, 352
BspDI ATCGAT 1 cut(s) 459
BspLI GGNNCC 1 cut(s) 250
BspMI ACCTGC 1 cut(s) 49
BspPI GGATC 1 cut(s) 13
BspQI GCTCTTC 1 cut(s) 348
BspTNI GGTCTC 1 cut(s) 215
BsrFI RCCGGY 1 cut(s) 526
BsrI ACTGG 2 cut(s) 45, 652
BssAI RCCGGY 1 cut(s) 526
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 4 cut(s) 5, 185, 456, 472
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 634
Bst6I CTCTTC 3 cut(s) 348, 498, 760
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 2 cut(s) 200, 360
BstENI CCTNNNNNAGG 1 cut(s) 711
BstF5I GGATG 3 cut(s) 252, 410, 688
BstHHI GCGC 2 cut(s) 306, 698
BstKTI GATC 4 cut(s) 8, 188, 459, 475
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 4 cut(s) 5, 185, 456, 472
BstMCI CGRYCG 1 cut(s) 531
BstNI CCWGG 1 cut(s) 243
BstNSI RCATGY 2 cut(s) 663, 705
BstSCI CCNGG 2 cut(s) 241, 733
BstV1I GCAGC 1 cut(s) 126
BstV2I GAAGAC 2 cut(s) 117, 710
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 1 cut(s) 383
BsuTUI ATCGAT 1 cut(s) 459
BtsCI GGATG 3 cut(s) 252, 410, 688
BtsIMutI CAGTG 1 cut(s) 630
BveI ACCTGC 1 cut(s) 49
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 2 cut(s) 306, 698
Cfr10I RCCGGY 1 cut(s) 526
ClaI ATCGAT 1 cut(s) 459
CviAII CATG 2 cut(s) 660, 702
DdeI CTNAG 2 cut(s) 200, 360
DpnI GATC 4 cut(s) 7, 187, 458, 474
DpnII GATC 4 cut(s) 5, 185, 456, 472
Eam1104I CTCTTC 3 cut(s) 348, 498, 760
EarI CTCTTC 3 cut(s) 348, 498, 760
Eco130I CCWWGG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 215
Eco57I CTGAAG 2 cut(s) 72, 618
EcoNI CCTNNNNNAGG 1 cut(s) 711
EcoRI GAATTC 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 2 cut(s) 663, 705
FaiI YATR 5 cut(s) 661, 703, 711, 779, 781
FalI AAGNNNNNCTT 2 cut(s) 553, 585
FaqI GGGAC 1 cut(s) 163
FatI CATG 2 cut(s) 659, 701
Fnu4HI GCNGC 2 cut(s) 140, 771
FokI GGATG 3 cut(s) 239, 397, 695
Fsp4HI GCNGC 2 cut(s) 140, 771
FspBI CTAG 3 cut(s) 257, 329, 677
GlaI GCGC 2 cut(s) 305, 697
GluI GCNGC 2 cut(s) 140, 771
GsaI CCCAGC 1 cut(s) 526
GsuI CTGGAG 1 cut(s) 393
HaeIII GGCC 1 cut(s) 383
HapII CCGG 2 cut(s) 527, 734
HhaI GCGC 2 cut(s) 306, 698
Hin1II CATG 2 cut(s) 663, 705
Hin6I GCGC 2 cut(s) 304, 696
HinP1I GCGC 2 cut(s) 304, 696
HindIII AAGCTT 1 cut(s) 567
HinfI GANTC 3 cut(s) 231, 375, 467
HpaII CCGG 2 cut(s) 527, 734
Hpy166II GTNNAC 3 cut(s) 101, 262, 674
Hpy188I TCNGA 6 cut(s) 91, 274, 361, 472, 477, 692
Hpy188III TCNNGA 5 cut(s) 199, 228, 235, 299, 372
Hpy8I GTNNAC 3 cut(s) 101, 262, 674
HpyAV CCTTC 4 cut(s) 211, 402, 441, 736
HpyCH4III ACNGT 1 cut(s) 634
HpyCH4V TGCA 5 cut(s) 62, 183, 454, 582, 770
HpyF3I CTNAG 2 cut(s) 200, 360
Hsp92II CATG 2 cut(s) 663, 705
HspAI GCGC 2 cut(s) 304, 696
Kzo9I GATC 4 cut(s) 5, 185, 456, 472
LguI GCTCTTC 1 cut(s) 348
LmnI GCTCC 2 cut(s) 83, 604
Lsp1109I GCAGC 1 cut(s) 126
LweI GCATC 4 cut(s) 19, 49, 673, 683
MaeI CTAG 3 cut(s) 257, 329, 677
MaeIII GTNAC 2 cut(s) 309, 621
MalI GATC 4 cut(s) 7, 187, 458, 474
MboI GATC 4 cut(s) 5, 185, 456, 472
MboII GAAGA 7 cut(s) 117, 147, 335, 515, 526, 710, 747
MfeI CAATTG 1 cut(s) 189
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 1 cut(s) 371
MluCI AATT 5 cut(s) 108, 189, 194, 402, 577
MlyI GAGTC 2 cut(s) 240, 384
MmeI TCCRAC 1 cut(s) 532
MnlI CCTC 8 cut(s) 64, 114, 235, 301, 478, 499, 585, 732
Mph1103I ATGCAT 1 cut(s) 64
MseI TTAA 3 cut(s) 65, 422, 497
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 1 cut(s) 139
MspI CCGG 2 cut(s) 527, 734
MspR9I CCNGG 2 cut(s) 243, 735
MunI CAATTG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 243
NciI CCSGG 1 cut(s) 735
NdeII GATC 4 cut(s) 5, 185, 456, 472
NlaIII CATG 2 cut(s) 663, 705
NlaIV GGNNCC 1 cut(s) 250
NsiI ATGCAT 1 cut(s) 64
NspI RCATGY 2 cut(s) 663, 705
OliI CACNNNNGTG 1 cut(s) 119
PciI ACATGT 1 cut(s) 701
PciSI GCTCTTC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 467
PkrI GCNGC 2 cut(s) 141, 772
PleI GAGTC 2 cut(s) 239, 383
PpsI GAGTC 2 cut(s) 239, 383
PscI ACATGT 1 cut(s) 701
PsiI TTATAA 1 cut(s) 711
Psp6I CCWGG 1 cut(s) 241
PspFI CCCAGC 1 cut(s) 522
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 1 cut(s) 250
PsuI RGATCY 1 cut(s) 472
PvuII CAGCTG 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 119
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 3 cut(s) 65, 422, 497
SatI GCNGC 2 cut(s) 140, 771
Sau3AI GATC 4 cut(s) 5, 185, 456, 472
SchI GAGTC 2 cut(s) 240, 384
ScrFI CCNGG 2 cut(s) 243, 735
SduI GDGCHC 1 cut(s) 371
SfaNI GCATC 4 cut(s) 19, 49, 673, 683
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 3 cut(s) 117, 226, 299
SmoI CTYRAG 3 cut(s) 117, 226, 299
Sse9I AATT 5 cut(s) 108, 189, 194, 402, 577
SsiI CCGC 1 cut(s) 32
SspMI CTAG 3 cut(s) 257, 329, 677
StyD4I CCNGG 2 cut(s) 241, 733
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 634
TaqI TCGA 2 cut(s) 162, 459
TasI AATT 5 cut(s) 108, 189, 194, 402, 577
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 3 cut(s) 65, 422, 497
Tru9I TTAA 3 cut(s) 65, 422, 497
TscAI CASTG 1 cut(s) 637
TseI GCWGC 2 cut(s) 139, 770
TspRI CASTG 1 cut(s) 637
XagI CCTNNNNNAGG 1 cut(s) 711
XapI RAATTY 1 cut(s) 194
XceI RCATGY 2 cut(s) 663, 705
XcmI CCANNNNNNNNNTGG 1 cut(s) 659
XspI CTAG 3 cut(s) 257, 329, 677
Zsp2I ATGCAT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.