MD16G1000500.v1.1

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S cluster from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Has anti- apoptotic effects in the cell

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008748
Physical Location & Seq
Forward (+)
27293 .. 30777
3485 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1000500.v1.1.491

Sequence Viewer

Length: 792 bp
ATGGGATCTGTTCTGGCATTTACAGATGATGGGGTTCTACCTGTTAGTCTAGTTTTTGATGCATTAAGGGAGCTCGGGACCGAAAAGTGTGAACCTCAAATTGTCACTCTGTCTTCTTCAAATCAGCTGCCGGTGGGGTCTTCCTCTGTGGACATTGTGTTTACTCTTTGTAGATCGATTGAATTTCCAAATGAGCAGTTGTTAGGGGAAGTCTCAAGAGTCTTGAAGCCTGGTGGAACAGTCCTAATTTACAAGATATCCGATGCTTCGACAGGGGAAACAGATAAGACCACCTCCGTTCTTGAGCGCAAGTTACTATTGTCTGGTTTTCTAGAAGCAAAAGCTCTTCAAGTGAAATCAAGTTTACCATCTGAGCTATCTTTTGGGGTCAAGGCTAAAAGGCCGTCTTGGAAGATTGGTTCATCCTTTGCCTTAAAAAAGACCACAAAAAATTTACCAAAAATTCAGATCAATGATGAGTCAGATCTCATTGACGAGGATAGTTTACTAACAGAAGAGGACTTGAAGAAACCCCAGCTGCTTTCTAGTGATTGCGAAGTTGGAAGCACAAGGAAAGCTTGTAAAAACTGCACTTGTGGGAGAGCTGAAGAAGAGCAGAAAGTAGAGAAGTTAGGCTCCACAGTAGATTTGAGCAATTTCAAGTCGCAATGTGGCAGTTGTGGACTAGGGGATGCTTTTCGATGCGCTACATGTCCTTTTAAAGGTCTTCCTCCATTCAAACCCGGCGAGAAGGTATCACTGTCAGCGAACTTCCTTACAGCAGACATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006790 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016226 GO:0022607 GO:0031163 GO:0044085 GO:0044237 GO:0044424 GO:0044464 GO:0051186 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIAPIN1 Methyltransf_11

Protein Analysis

264

Amino Acids

28.29

Weight (kDa)

5.34

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anamorsin_N PF20922 45 - 126 3.2e-08 Anamorsin, N-terminal
CIAPIN1 PF05093 217 - 254 5.3e-15 Cytokine-induced anti-apoptosis inhibitor 1, Fe-S biogenesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 13
AcsI RAATTY 3 cut(s) 182, 451, 462
AcuI CTGAAG 1 cut(s) 627
AfiI CCNNNNNNNGG 1 cut(s) 722
AflIII ACRYGT 1 cut(s) 710
AgsI TTSAA 7 cut(s) 120, 182, 226, 350, 526, 661, 739
AjnI CCWGG 1 cut(s) 229
AloI GAACNNNNNNTCC 2 cut(s) 403, 435
AluBI AGCT 7 cut(s) 73, 127, 344, 376, 538, 578, 605
AluI AGCT 7 cut(s) 73, 127, 344, 376, 538, 578, 605
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 1 cut(s) 217
AlwI GGATC 1 cut(s) 13
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 1 cut(s) 401
ApeKI GCWGC 2 cut(s) 127, 538
ApoI RAATTY 3 cut(s) 182, 451, 462
AspLEI GCGC 2 cut(s) 309, 707
AspS9I GGNCC 1 cut(s) 78
AsuC2I CCSGG 1 cut(s) 744
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 1 cut(s) 78
BanII GRGCYC 1 cut(s) 75
BbsI GAAGAC 3 cut(s) 105, 132, 719
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 2 cut(s) 114, 525
BccI CCATC 2 cut(s) 23, 376
BceAI ACGGC 1 cut(s) 388
BciT130I CCWGG 1 cut(s) 231
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 1 cut(s) 217
BfaI CTAG 5 cut(s) 50, 332, 546, 686, 790
BglII AGATCT 1 cut(s) 484
BisI GCNGC 2 cut(s) 128, 539
BlsI GCNGC 2 cut(s) 129, 540
Bme1390I CCNGG 2 cut(s) 231, 744
Bme18I GGWCC 1 cut(s) 78
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 78
BmiI GGNNCC 2 cut(s) 79, 637
BmrFI CCNGG 2 cut(s) 231, 744
BmsI GCATC 4 cut(s) 49, 253, 682, 692
BpiI GAAGAC 3 cut(s) 105, 132, 719
BpuEI CTTGAG 2 cut(s) 199, 323
BpuMI CCSGG 1 cut(s) 744
Bsa29I ATCGAT 1 cut(s) 176
Bsc4I CCNNNNNNNGG 1 cut(s) 722
Bse118I RCCGGY 1 cut(s) 130
Bse3DI GCAATG 1 cut(s) 674
BseBI CCWGG 1 cut(s) 231
BseCI ATCGAT 1 cut(s) 176
BseGI GGATG 2 cut(s) 422, 697
BseLI CCNNNNNNNGG 1 cut(s) 722
BseMI GCAATG 1 cut(s) 674
BseMII CTCAG 1 cut(s) 363
BseXI GCAGC 2 cut(s) 114, 525
BseYI CCCAGC 1 cut(s) 534
BsgI GTGCAG 1 cut(s) 574
BshFI GGCC 1 cut(s) 403
BshVI ATCGAT 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 75
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 2 cut(s) 131, 744
BslFI GGGAC 1 cut(s) 91
BslI CCNNNNNNNGG 1 cut(s) 722
BsmAI GTCTC 1 cut(s) 217
BsmFI GGGAC 1 cut(s) 91
BsnI GGCC 1 cut(s) 403
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 4 cut(s) 5, 173, 468, 484
BspANI GGCC 1 cut(s) 403
BspCNI CTCAG 1 cut(s) 364
BspDI ATCGAT 1 cut(s) 176
BspLI GGNNCC 2 cut(s) 79, 637
BspPI GGATC 1 cut(s) 13
BspQI GCTCTTC 2 cut(s) 351, 606
BsrDI GCAATG 1 cut(s) 674
BsrFI RCCGGY 1 cut(s) 130
BssAI RCCGGY 1 cut(s) 130
BssMI GATC 4 cut(s) 5, 173, 468, 484
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 3 cut(s) 241, 643, 762
Bst6I CTCTTC 3 cut(s) 351, 510, 606
BstDEI CTNAG 1 cut(s) 372
BstENI CCTNNNNNAGG 1 cut(s) 720
BstF5I GGATG 2 cut(s) 422, 697
BstHHI GCGC 2 cut(s) 309, 707
BstKTI GATC 4 cut(s) 8, 176, 471, 487
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 4 cut(s) 5, 173, 468, 484
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 1 cut(s) 714
BstSCI CCNGG 2 cut(s) 229, 742
BstV1I GCAGC 2 cut(s) 114, 525
BstV2I GAAGAC 3 cut(s) 105, 132, 719
BstX2I RGATCY 2 cut(s) 5, 484
BstYI RGATCY 2 cut(s) 5, 484
Bsu15I ATCGAT 1 cut(s) 176
BsuRI GGCC 1 cut(s) 403
BsuTUI ATCGAT 1 cut(s) 176
BtsCI GGATG 2 cut(s) 422, 697
BtsIMutI CAGTG 1 cut(s) 758
CfoI GCGC 2 cut(s) 309, 707
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 78
ClaI ATCGAT 1 cut(s) 176
CviAII CATG 1 cut(s) 711
DdeI CTNAG 1 cut(s) 372
DpnI GATC 4 cut(s) 7, 175, 470, 486
DpnII GATC 4 cut(s) 5, 173, 468, 484
DraI TTTAAA 1 cut(s) 721
Eam1104I CTCTTC 3 cut(s) 351, 510, 606
EarI CTCTTC 3 cut(s) 351, 510, 606
Ecl136II GAGCTC 1 cut(s) 73
Eco24I GRGCYC 1 cut(s) 75
Eco32I GATATC 1 cut(s) 258
Eco47I GGWCC 1 cut(s) 78
Eco53kI GAGCTC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 627
Eco88I CYCGRG 1 cut(s) 74
EcoICRI GAGCTC 1 cut(s) 73
EcoNI CCTNNNNNAGG 1 cut(s) 720
EcoRII CCWGG 1 cut(s) 229
EcoRV GATATC 1 cut(s) 258
EcoT22I ATGCAT 1 cut(s) 64
EcoT38I GRGCYC 1 cut(s) 75
FaeI CATG 1 cut(s) 714
FaiI YATR 1 cut(s) 712
FalI AAGNNNNNCTT 4 cut(s) 391, 423, 562, 594
FaqI GGGAC 1 cut(s) 91
FatI CATG 1 cut(s) 710
Fnu4HI GCNGC 2 cut(s) 128, 539
FokI GGATG 2 cut(s) 409, 704
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 2 cut(s) 128, 539
FspBI CTAG 5 cut(s) 50, 332, 546, 686, 790
GlaI GCGC 2 cut(s) 308, 706
GluI GCNGC 2 cut(s) 128, 539
GsaI CCCAGC 1 cut(s) 538
HaeIII GGCC 1 cut(s) 403
HapII CCGG 2 cut(s) 131, 744
HhaI GCGC 2 cut(s) 309, 707
Hin1II CATG 1 cut(s) 714
Hin6I GCGC 2 cut(s) 307, 705
HinP1I GCGC 2 cut(s) 307, 705
HindIII AAGCTT 1 cut(s) 576
HinfI GANTC 2 cut(s) 219, 479
HpaII CCGG 2 cut(s) 131, 744
Hpy166II GTNNAC 6 cut(s) 92, 151, 162, 365, 506, 683
Hpy188I TCNGA 4 cut(s) 262, 373, 468, 484
Hpy188III TCNNGA 5 cut(s) 76, 216, 223, 302, 332
Hpy8I GTNNAC 6 cut(s) 92, 151, 162, 365, 506, 683
HpyAV CCTTC 1 cut(s) 745
HpyCH4III ACNGT 3 cut(s) 241, 643, 762
HpyCH4V TGCA 2 cut(s) 62, 591
HpyF3I CTNAG 1 cut(s) 372
Hsp92II CATG 1 cut(s) 714
HspAI GCGC 2 cut(s) 307, 705
Kzo9I GATC 4 cut(s) 5, 173, 468, 484
LguI GCTCTTC 2 cut(s) 351, 606
LmnI GCTCC 2 cut(s) 70, 641
LpnPI CCDG 8 cut(s) 54, 144, 216, 243, 258, 309, 548, 757
Lsp1109I GCAGC 2 cut(s) 114, 525
LweI GCATC 4 cut(s) 49, 253, 682, 692
MaeI CTAG 5 cut(s) 50, 332, 546, 686, 790
MaeIII GTNAC 2 cut(s) 103, 312
MalI GATC 4 cut(s) 7, 175, 470, 486
MboI GATC 4 cut(s) 5, 173, 468, 484
MflI RGATCY 2 cut(s) 5, 484
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 6 cut(s) 99, 182, 246, 451, 462, 655
MlyI GAGTC 2 cut(s) 228, 488
MmeI TCCRAC 1 cut(s) 541
MnlI CCTC 6 cut(s) 105, 154, 304, 490, 511, 741
Mph1103I ATGCAT 1 cut(s) 64
MseI TTAA 3 cut(s) 65, 434, 720
MspA1I CMGCKG 2 cut(s) 127, 538
MspI CCGG 2 cut(s) 131, 744
MspR9I CCNGG 2 cut(s) 231, 744
MvaI CCWGG 1 cut(s) 231
NciI CCSGG 1 cut(s) 744
NdeII GATC 4 cut(s) 5, 173, 468, 484
NlaIII CATG 1 cut(s) 714
NlaIV GGNNCC 2 cut(s) 79, 637
NmuCI GTSAC 1 cut(s) 103
NsiI ATGCAT 1 cut(s) 64
NspI RCATGY 1 cut(s) 714
PciI ACATGT 1 cut(s) 710
PciSI GCTCTTC 2 cut(s) 351, 606
PkrI GCNGC 2 cut(s) 129, 540
PleI GAGTC 2 cut(s) 227, 487
PpsI GAGTC 2 cut(s) 227, 487
PscI ACATGT 1 cut(s) 710
Psp124BI GAGCTC 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 229
PspFI CCCAGC 1 cut(s) 534
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 2 cut(s) 79, 637
PspPI GGNCC 1 cut(s) 78
PsuI RGATCY 2 cut(s) 5, 484
PvuII CAGCTG 2 cut(s) 127, 538
SacI GAGCTC 1 cut(s) 75
SapI GCTCTTC 2 cut(s) 351, 606
SaqAI TTAA 3 cut(s) 65, 434, 720
SatI GCNGC 2 cut(s) 128, 539
Sau3AI GATC 4 cut(s) 5, 173, 468, 484
Sau96I GGNCC 1 cut(s) 78
SchI GAGTC 2 cut(s) 228, 488
ScrFI CCNGG 2 cut(s) 231, 744
SduI GDGCHC 1 cut(s) 75
SfaNI GCATC 4 cut(s) 49, 253, 682, 692
SinI GGWCC 1 cut(s) 78
SmlI CTYRAG 2 cut(s) 214, 302
SmoI CTYRAG 2 cut(s) 214, 302
Sse9I AATT 6 cut(s) 99, 182, 246, 451, 462, 655
SspMI CTAG 5 cut(s) 50, 332, 546, 686, 790
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 2 cut(s) 229, 742
TaaI ACNGT 3 cut(s) 241, 643, 762
TaqI TCGA 3 cut(s) 176, 269, 700
TaqII GACCGA 1 cut(s) 95
TasI AATT 6 cut(s) 99, 182, 246, 451, 462, 655
Tru1I TTAA 3 cut(s) 65, 434, 720
Tru9I TTAA 3 cut(s) 65, 434, 720
TscAI CASTG 1 cut(s) 765
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 2 cut(s) 127, 538
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 1 cut(s) 411
TspGWI ACGGA 1 cut(s) 286
TspRI CASTG 1 cut(s) 765
VpaK11BI GGWCC 1 cut(s) 78
XagI CCTNNNNNAGG 1 cut(s) 720
XapI RAATTY 3 cut(s) 182, 451, 462
XbaI TCTAGA 1 cut(s) 331
XceI RCATGY 1 cut(s) 714
XspI CTAG 5 cut(s) 50, 332, 546, 686, 790
Zsp2I ATGCAT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.