Rh4DG441000

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S cluster from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Has anti- apoptotic effects in the cell

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008748
Physical Location & Seq
Forward (+)
64255787 .. 64258789
3003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG441000.1

Sequence Viewer

Length: 579 bp
ATGGGATCGGTGCTGGCATTTACAGATGATGCGGTTCTACCTGCCAGTCTTGTTTTTGATGCATTAAGGGAGCTTGGGAATGGAGCTTCAGATAAGTGTGAACCTCAAATTATCACTCAAGTGTCTTCCTTGGGTCAGCTGCCACAGGGGACTTCCTCTATCAACATAGTGTTTGCCCTTTGCAGATCAATTGAAATCCTGAGTGAGCAGTTGTTGAAGGAGACCTCAAGAGTCTTGAAACCTGGTGGAACAATCCTAGTTTACAAGACTTCTGAAACAGATAAGACCACCTCTGTTCTTGAGCGCAAGTTACTTTTGTCTGGTTTCCTAGAAGCACAAGCTCTTCAAACAAAATCATCTGAGTTGTGCTCTGGAGTCAAGGCCAAATTGCCTTCTTGGAAAATTGGCTCATCCTTTGCCTTAAAAAAGCCTTCAAAAACTCTACCAAAGTTGCAGATCGATGATGATTCAGATCTGATTGATGAGGATAGTCTATTAACGGAAGAGGACTTGAAGAAACCCCAGCCGGTCGTAGGTAAGACATTGTTATACAAAAATAATTGTCTGTTGATCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006790 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016226 GO:0022607 GO:0031163 GO:0044085 GO:0044237 GO:0044424 GO:0044464 GO:0051186 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIAPIN1 Methyltransf_11

Protein Analysis

192

Amino Acids

20.79

Weight (kDa)

5.33

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anamorsin_N PF20922 39 - 119 5.6e-07 Anamorsin, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 49
AciI CCGC 1 cut(s) 32
AclWI GGATC 1 cut(s) 13
AcuI CTGAAG 1 cut(s) 72
AfiI CCNNNNNNNGG 1 cut(s) 533
AgsI TTSAA 6 cut(s) 194, 217, 238, 347, 435, 514
AjnI CCWGG 1 cut(s) 241
AleI CACNNNNGTG 1 cut(s) 119
AluBI AGCT 4 cut(s) 73, 86, 139, 341
AluI AGCT 4 cut(s) 73, 86, 139, 341
Alw21I GWGCWC 1 cut(s) 371
Alw26I GTCTC 1 cut(s) 215
AlwI GGATC 1 cut(s) 13
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 1 cut(s) 139
AspLEI GCGC 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 371
BbvI GCAGC 1 cut(s) 126
BciT130I CCWGG 1 cut(s) 243
BclI TGATCA 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 215
BfaI CTAG 2 cut(s) 257, 329
BfuAI ACCTGC 1 cut(s) 49
BglII AGATCT 1 cut(s) 472
BisI GCNGC 1 cut(s) 140
BlsI GCNGC 1 cut(s) 141
Bme1390I CCNGG 1 cut(s) 243
BmrFI CCNGG 1 cut(s) 243
BmsI GCATC 2 cut(s) 19, 49
BpiI GAAGAC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpmI CTGGAG 1 cut(s) 393
BpuEI CTTGAG 3 cut(s) 102, 211, 320
Bsa29I ATCGAT 1 cut(s) 459
BsaBI GATNNNNATC 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 129
Bsc4I CCNNNNNNNGG 1 cut(s) 533
Bse118I RCCGGY 1 cut(s) 526
Bse1I ACTGG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 1 cut(s) 243
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 1 cut(s) 410
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 1 cut(s) 533
BseMII CTCAG 2 cut(s) 191, 351
BseNI ACTGG 1 cut(s) 45
BseXI GCAGC 1 cut(s) 126
BseYI CCCAGC 1 cut(s) 522
Bsh1285I CGRYCG 1 cut(s) 531
BshFI GGCC 1 cut(s) 383
BshVI ATCGAT 1 cut(s) 459
BsiEI CGRYCG 1 cut(s) 531
BsiHKAI GWGCWC 1 cut(s) 371
BsiSI CCGG 1 cut(s) 527
BslFI GGGAC 1 cut(s) 163
BslI CCNNNNNNNGG 1 cut(s) 533
BsmAI GTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 163
BsnI GGCC 1 cut(s) 383
Bso31I GGTCTC 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 371
Bsp143I GATC 5 cut(s) 5, 185, 456, 472, 570
BspACI CCGC 1 cut(s) 32
BspANI GGCC 1 cut(s) 383
BspCNI CTCAG 2 cut(s) 192, 352
BspDI ATCGAT 1 cut(s) 459
BspMI ACCTGC 1 cut(s) 49
BspPI GGATC 1 cut(s) 13
BspQI GCTCTTC 1 cut(s) 348
BspTNI GGTCTC 1 cut(s) 215
BsrFI RCCGGY 1 cut(s) 526
BsrI ACTGG 1 cut(s) 45
BssAI RCCGGY 1 cut(s) 526
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 5 cut(s) 5, 185, 456, 472, 570
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 243
Bst6I CTCTTC 2 cut(s) 348, 498
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 2 cut(s) 200, 360
BstF5I GGATG 1 cut(s) 410
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 5 cut(s) 8, 188, 459, 475, 573
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 5 cut(s) 5, 185, 456, 472, 570
BstMCI CGRYCG 1 cut(s) 531
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 126
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 1 cut(s) 383
BsuTUI ATCGAT 1 cut(s) 459
BtsCI GGATG 1 cut(s) 410
BveI ACCTGC 1 cut(s) 49
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 1 cut(s) 306
Cfr10I RCCGGY 1 cut(s) 526
ClaI ATCGAT 1 cut(s) 459
CsiI ACCWGGT 1 cut(s) 241
CviJI RGCY 8 cut(s) 73, 86, 139, 341, 383, 408, 430, 526
CviKI_1 RGCY 8 cut(s) 73, 86, 139, 341, 383, 408, 430, 526
DdeI CTNAG 2 cut(s) 200, 360
DpnI GATC 5 cut(s) 7, 187, 458, 474, 572
DpnII GATC 5 cut(s) 5, 185, 456, 472, 570
Eam1104I CTCTTC 2 cut(s) 348, 498
EarI CTCTTC 2 cut(s) 348, 498
Eco130I CCWWGG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 72
EcoRII CCWGG 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 129
FaiI YATR 2 cut(s) 167, 550
FaqI GGGAC 1 cut(s) 163
FbaI TGATCA 1 cut(s) 570
Fnu4HI GCNGC 1 cut(s) 140
FokI GGATG 1 cut(s) 397
Fsp4HI GCNGC 1 cut(s) 140
FspBI CTAG 2 cut(s) 257, 329
GlaI GCGC 1 cut(s) 305
GluI GCNGC 1 cut(s) 140
GsaI CCCAGC 1 cut(s) 526
GsuI CTGGAG 1 cut(s) 393
HaeIII GGCC 1 cut(s) 383
HapII CCGG 1 cut(s) 527
HhaI GCGC 1 cut(s) 306
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HinfI GANTC 3 cut(s) 231, 375, 467
HpaII CCGG 1 cut(s) 527
Hpy166II GTNNAC 2 cut(s) 101, 262
Hpy188I TCNGA 5 cut(s) 91, 274, 361, 472, 477
Hpy188III TCNNGA 5 cut(s) 199, 228, 235, 299, 372
Hpy8I GTNNAC 2 cut(s) 101, 262
HpyAV CCTTC 3 cut(s) 211, 402, 441
HpyCH4V TGCA 3 cut(s) 62, 183, 454
HpyF3I CTNAG 2 cut(s) 200, 360
HspAI GCGC 1 cut(s) 304
Ksp22I TGATCA 1 cut(s) 570
Kzo9I GATC 5 cut(s) 5, 185, 456, 472, 570
LguI GCTCTTC 1 cut(s) 348
LmnI GCTCC 2 cut(s) 70, 83
Lsp1109I GCAGC 1 cut(s) 126
LweI GCATC 2 cut(s) 19, 49
MabI ACCWGGT 1 cut(s) 241
MaeI CTAG 2 cut(s) 257, 329
MaeIII GTNAC 1 cut(s) 309
MalI GATC 5 cut(s) 7, 187, 458, 474, 572
MboI GATC 5 cut(s) 5, 185, 456, 472, 570
MboII GAAGA 4 cut(s) 117, 335, 515, 526
MfeI CAATTG 1 cut(s) 189
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 1 cut(s) 371
MluCI AATT 5 cut(s) 108, 189, 386, 402, 559
MlyI GAGTC 2 cut(s) 240, 384
MnlI CCTC 6 cut(s) 114, 166, 235, 301, 478, 499
Mph1103I ATGCAT 1 cut(s) 64
MseI TTAA 3 cut(s) 65, 422, 497
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 1 cut(s) 139
MspI CCGG 1 cut(s) 527
MspR9I CCNGG 1 cut(s) 243
MunI CAATTG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 243
NdeII GATC 5 cut(s) 5, 185, 456, 472, 570
NsiI ATGCAT 1 cut(s) 64
OliI CACNNNNGTG 1 cut(s) 119
PciSI GCTCTTC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 467
PkrI GCNGC 1 cut(s) 141
PleI GAGTC 2 cut(s) 239, 383
PpsI GAGTC 2 cut(s) 239, 383
Psp6I CCWGG 1 cut(s) 241
PspFI CCCAGC 1 cut(s) 522
PspGI CCWGG 1 cut(s) 241
PsuI RGATCY 1 cut(s) 472
PvuII CAGCTG 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 119
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 3 cut(s) 65, 422, 497
SatI GCNGC 1 cut(s) 140
Sau3AI GATC 5 cut(s) 5, 185, 456, 472, 570
SchI GAGTC 2 cut(s) 240, 384
ScrFI CCNGG 1 cut(s) 243
SduI GDGCHC 1 cut(s) 371
SexAI ACCWGGT 1 cut(s) 241
SfaNI GCATC 2 cut(s) 19, 49
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 3 cut(s) 117, 226, 299
SmoI CTYRAG 3 cut(s) 117, 226, 299
Sse9I AATT 5 cut(s) 108, 189, 386, 402, 559
SsiI CCGC 1 cut(s) 32
SspMI CTAG 2 cut(s) 257, 329
StyD4I CCNGG 1 cut(s) 241
StyI CCWWGG 1 cut(s) 129
TaqI TCGA 1 cut(s) 459
TasI AATT 5 cut(s) 108, 189, 386, 402, 559
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 3 cut(s) 65, 422, 497
Tru9I TTAA 3 cut(s) 65, 422, 497
TseI GCWGC 1 cut(s) 139
TspGWI ACGGA 1 cut(s) 515
XspI CTAG 2 cut(s) 257, 329
Zsp2I ATGCAT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.