Rh4CG459600

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S cluster from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Has anti- apoptotic effects in the cell

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008748
Physical Location & Seq
Forward (+)
69080344 .. 69082233
1890 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG459600.1

Sequence Viewer

Length: 612 bp
ATGGGATCGGTGCTGGCATTTACAGATGATGCGGTTCTACCTGCCAGTCTTGTTTTTGATGCATTAAGGGAGGTTGGGAATGGAGCTTCAGATAAGTGTGAACCTCAAATTATCACTCAAGTGTCTTCCTTGGGTCAGCTGCCACAGGGGACTTCTTCTATCGACATTGTGTTTGCCCTTTGCAGATCAATTGAATTCCTGAGTGAGCAGTTGTTGAAGGAGACCTCAAGAGTCTTGAAGCCTGGTGGAACCATCCTAGTTTACAAGACTTCTGAAACAGATAAGACCACCTCTGTTCTTGAGCGCAAGTTACTTTTGTCTGGTTTCCTAGAAGCACAAGCTCTTCAAACAAAATCATCTGAGTTGTGCTCTGGAGTCAAGGCCAAAAAGCCTTCTTGGAAAATTGGCTCATCCTTTGCCTTAAAAAAGCCTTCAAAAACTCTACCGAAGTTGCAGATCGATGATGATTCAGATCTGATTGATGAGGATAGTCTATTAACAGAAGAGGACTTGAAGAAACCCCAGCCGGTCCTGATGATTGTGAAGTTGGAAGCACAAGGAAAGCTTGTAAAAATTGCACTTGTGGGAGGGCTGAAGTGGAGCAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006790 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016226 GO:0022607 GO:0031163 GO:0044085 GO:0044237 GO:0044424 GO:0044464 GO:0051186 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIAPIN1 Methyltransf_11

Protein Analysis

203

Amino Acids

21.98

Weight (kDa)

7.6

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anamorsin_N PF20922 39 - 119 1.1e-06 Anamorsin, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 49
AciI CCGC 1 cut(s) 32
AclWI GGATC 1 cut(s) 13
AcsI RAATTY 1 cut(s) 194
AcuI CTGAAG 1 cut(s) 72
AgsI TTSAA 6 cut(s) 194, 217, 238, 347, 435, 514
AjnI CCWGG 1 cut(s) 241
AleI CACNNNNGTG 1 cut(s) 119
AluBI AGCT 4 cut(s) 86, 139, 341, 565
AluI AGCT 4 cut(s) 86, 139, 341, 565
Alw21I GWGCWC 1 cut(s) 371
Alw26I GTCTC 1 cut(s) 215
AlwI GGATC 1 cut(s) 13
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 1 cut(s) 139
ApoI RAATTY 1 cut(s) 194
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 1 cut(s) 529
AvaII GGWCC 1 cut(s) 529
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 371
BbvI GCAGC 1 cut(s) 126
BccI CCATC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 243
BcoDI GTCTC 1 cut(s) 215
BfaI CTAG 2 cut(s) 257, 329
BfuAI ACCTGC 1 cut(s) 49
BglII AGATCT 1 cut(s) 472
BisI GCNGC 1 cut(s) 140
BlsI GCNGC 1 cut(s) 141
Bme1390I CCNGG 1 cut(s) 243
Bme18I GGWCC 1 cut(s) 529
BmgT120I GGNCC 1 cut(s) 529
BmiI GGNNCC 1 cut(s) 250
BmrFI CCNGG 1 cut(s) 243
BmsI GCATC 2 cut(s) 19, 49
BpiI GAAGAC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpmI CTGGAG 1 cut(s) 393
BpuEI CTTGAG 3 cut(s) 102, 211, 320
Bsa29I ATCGAT 1 cut(s) 459
BsaBI GATNNNNATC 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 129
Bse118I RCCGGY 1 cut(s) 526
Bse1I ACTGG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 1 cut(s) 243
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 2 cut(s) 252, 410
BseJI GATNNNNATC 1 cut(s) 471
BseMII CTCAG 2 cut(s) 191, 351
BseNI ACTGG 1 cut(s) 45
BseXI GCAGC 1 cut(s) 126
BseYI CCCAGC 1 cut(s) 522
BshFI GGCC 1 cut(s) 383
BshVI ATCGAT 1 cut(s) 459
BsiHKAI GWGCWC 1 cut(s) 371
BsiSI CCGG 1 cut(s) 527
BslFI GGGAC 1 cut(s) 163
BsmAI GTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 163
BsnI GGCC 1 cut(s) 383
Bso31I GGTCTC 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 371
Bsp143I GATC 4 cut(s) 5, 185, 456, 472
BspACI CCGC 1 cut(s) 32
BspANI GGCC 1 cut(s) 383
BspCNI CTCAG 2 cut(s) 192, 352
BspDI ATCGAT 1 cut(s) 459
BspLI GGNNCC 1 cut(s) 250
BspMI ACCTGC 1 cut(s) 49
BspPI GGATC 1 cut(s) 13
BspQI GCTCTTC 1 cut(s) 348
BspTNI GGTCTC 1 cut(s) 215
BsrFI RCCGGY 1 cut(s) 526
BsrI ACTGG 1 cut(s) 45
BssAI RCCGGY 1 cut(s) 526
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 4 cut(s) 5, 185, 456, 472
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 243
Bst6I CTCTTC 2 cut(s) 348, 498
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 2 cut(s) 200, 360
BstF5I GGATG 2 cut(s) 252, 410
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 4 cut(s) 8, 188, 459, 475
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 4 cut(s) 5, 185, 456, 472
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 126
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 1 cut(s) 383
BsuTUI ATCGAT 1 cut(s) 459
BtsCI GGATG 2 cut(s) 252, 410
BveI ACCTGC 1 cut(s) 49
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 1 cut(s) 306
Cfr10I RCCGGY 1 cut(s) 526
Cfr13I GGNCC 1 cut(s) 529
ClaI ATCGAT 1 cut(s) 459
DdeI CTNAG 2 cut(s) 200, 360
DpnI GATC 4 cut(s) 7, 187, 458, 474
DpnII GATC 4 cut(s) 5, 185, 456, 472
Eam1104I CTCTTC 2 cut(s) 348, 498
EarI CTCTTC 2 cut(s) 348, 498
Eco130I CCWWGG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 215
Eco47I GGWCC 1 cut(s) 529
Eco57I CTGAAG 1 cut(s) 72
EcoRI GAATTC 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 129
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FaqI GGGAC 1 cut(s) 163
Fnu4HI GCNGC 1 cut(s) 140
FokI GGATG 2 cut(s) 239, 397
Fsp4HI GCNGC 1 cut(s) 140
FspBI CTAG 2 cut(s) 257, 329
GlaI GCGC 1 cut(s) 305
GluI GCNGC 1 cut(s) 140
GsaI CCCAGC 1 cut(s) 526
GsuI CTGGAG 1 cut(s) 393
HaeIII GGCC 1 cut(s) 383
HapII CCGG 1 cut(s) 527
HhaI GCGC 1 cut(s) 306
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HindIII AAGCTT 1 cut(s) 563
HinfI GANTC 3 cut(s) 231, 375, 467
HpaII CCGG 1 cut(s) 527
Hpy166II GTNNAC 2 cut(s) 101, 262
Hpy188I TCNGA 5 cut(s) 91, 274, 361, 472, 477
Hpy188III TCNNGA 6 cut(s) 199, 228, 235, 299, 372, 532
Hpy8I GTNNAC 2 cut(s) 101, 262
HpyAV CCTTC 3 cut(s) 211, 402, 441
HpyCH4V TGCA 4 cut(s) 62, 183, 454, 578
HpyF3I CTNAG 2 cut(s) 200, 360
HspAI GCGC 1 cut(s) 304
Kzo9I GATC 4 cut(s) 5, 185, 456, 472
LguI GCTCTTC 1 cut(s) 348
LmnI GCTCC 2 cut(s) 83, 600
Lsp1109I GCAGC 1 cut(s) 126
LweI GCATC 2 cut(s) 19, 49
MaeI CTAG 2 cut(s) 257, 329
MaeIII GTNAC 1 cut(s) 309
MalI GATC 4 cut(s) 7, 187, 458, 474
MboI GATC 4 cut(s) 5, 185, 456, 472
MboII GAAGA 5 cut(s) 117, 147, 335, 515, 526
MfeI CAATTG 1 cut(s) 189
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 1 cut(s) 371
MluCI AATT 5 cut(s) 108, 189, 194, 402, 573
MlyI GAGTC 2 cut(s) 240, 384
MmeI TCCRAC 1 cut(s) 528
MnlI CCTC 7 cut(s) 64, 114, 235, 301, 478, 499, 581
Mph1103I ATGCAT 1 cut(s) 64
MseI TTAA 3 cut(s) 65, 422, 497
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 1 cut(s) 139
MspI CCGG 1 cut(s) 527
MspR9I CCNGG 1 cut(s) 243
MunI CAATTG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 243
NdeII GATC 4 cut(s) 5, 185, 456, 472
NlaIV GGNNCC 1 cut(s) 250
NsiI ATGCAT 1 cut(s) 64
OliI CACNNNNGTG 1 cut(s) 119
PciSI GCTCTTC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 467
PkrI GCNGC 1 cut(s) 141
PleI GAGTC 2 cut(s) 239, 383
PpsI GAGTC 2 cut(s) 239, 383
Psp6I CCWGG 1 cut(s) 241
PspFI CCCAGC 1 cut(s) 522
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 1 cut(s) 250
PspPI GGNCC 1 cut(s) 529
PsuI RGATCY 1 cut(s) 472
PvuII CAGCTG 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 119
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 3 cut(s) 65, 422, 497
SatI GCNGC 1 cut(s) 140
Sau3AI GATC 4 cut(s) 5, 185, 456, 472
Sau96I GGNCC 1 cut(s) 529
SchI GAGTC 2 cut(s) 240, 384
ScrFI CCNGG 1 cut(s) 243
SduI GDGCHC 1 cut(s) 371
SetI ASST 9 cut(s) 43, 75, 88, 106, 141, 227, 293, 343, 567
SfaNI GCATC 2 cut(s) 19, 49
SinI GGWCC 1 cut(s) 529
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 3 cut(s) 117, 226, 299
SmoI CTYRAG 3 cut(s) 117, 226, 299
Sse9I AATT 5 cut(s) 108, 189, 194, 402, 573
SsiI CCGC 1 cut(s) 32
SspMI CTAG 2 cut(s) 257, 329
StyD4I CCNGG 1 cut(s) 241
StyI CCWWGG 1 cut(s) 129
TaqI TCGA 2 cut(s) 162, 459
TasI AATT 5 cut(s) 108, 189, 194, 402, 573
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 3 cut(s) 65, 422, 497
Tru9I TTAA 3 cut(s) 65, 422, 497
TseI GCWGC 1 cut(s) 139
VpaK11BI GGWCC 1 cut(s) 529
XapI RAATTY 1 cut(s) 194
XspI CTAG 2 cut(s) 257, 329
Zsp2I ATGCAT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.