Prupe.1G345400_v2.0.a1

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S cluster from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Has anti- apoptotic effects in the cell

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0008748
Physical Location & Seq
Forward (+)
32321592 .. 32324871
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G345400.1

Sequence Viewer

Length: 807 bp
ATGGCTTCCGTGGTGGCATTTACAGATGATGCGGTTCTTCCTGTAAGTCTTGTTTTTGATGCATTGAAGGAGCTTGGGAATGGAGCTACTGAAAAGTGCGAACCTCAAATTATGACCGAAGTGTCTTCTTTAAGTCAGCTGCCCCTGGGGTCTTCCTCTGTGGACGTTGTTTTTACTCTTTGTAAATCGATTGAATTTCCAAGTGAGCAGTTGTTAGGGGAAATCTCAAGGGTCTTGAAGCCTGGTGGATCAGTCCTAATTTACAAGACTTCTGATTCTTCCACAGGGGAAAGTGATAAGACCACCTCTGTTATTGAGCGCAAGTTACTATTGTCTGGTTTTCTAGAAGCACAAGCTCTTCAAGAGAAATCAAATTTACCATCTGAGTTGTCCTTTGGGGTCAAGGCTAAAAAACCTTCCTGGAAGATTGGTTCATCCTTTGCCTTAAAAAAGACCACCAAAAGTTTACCAAAAGTTCAGATCAATGATGATTCAGATCTGATTGATGAGGATAGTCTATTAACAGAGGAGGACTTAAAGAAACCCCAGCCGCTTCTAGCTGGTGATTGTGAAGTTGGAAGCACAAGGAAAGCTTGTAAAAATTGCACTTGTGGGAGGGCTGAAGAAGAGCAGAAAGTGGAGAAGTTAGGCTCAACGGTAGATTTGGACAATTTCAAGTCGCAATGTGGCAGCTGTGGACTAGGCGATGCTTTTCGATGCGCTACATGTCCTTTTAAAGGTCTTCCTCCATTCAAACCTGGAGAGAAGGTATCTTTGTCAGCGAACTTCCTTGCAGCAGACATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006790 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016226 GO:0022607 GO:0031163 GO:0044085 GO:0044237 GO:0044424 GO:0044464 GO:0051186 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIAPIN1 Methyltransf_11

Protein Analysis

269

Amino Acids

28.63

Weight (kDa)

4.95

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 121
AciI CCGC 2 cut(s) 32, 551
AclWI GGATC 1 cut(s) 256
AcsI RAATTY 2 cut(s) 194, 373
AcuI CTGAAG 1 cut(s) 642
AfiI CCNNNNNNNGG 1 cut(s) 737
AflIII ACRYGT 1 cut(s) 725
AgsI TTSAA 6 cut(s) 67, 194, 238, 362, 676, 754
AjnI CCWGG 4 cut(s) 144, 241, 419, 757
AloI GAACNNNNNNTCC 2 cut(s) 415, 447
AluBI AGCT 7 cut(s) 73, 86, 139, 356, 560, 593, 693
AluI AGCT 7 cut(s) 73, 86, 139, 356, 560, 593, 693
AlwI GGATC 1 cut(s) 256
ApeKI GCWGC 3 cut(s) 139, 690, 794
ApoI RAATTY 2 cut(s) 194, 373
AspLEI GCGC 2 cut(s) 321, 722
AsuHPI GGTGA 1 cut(s) 575
BbsI GAAGAC 3 cut(s) 117, 144, 734
BbvI GCAGC 2 cut(s) 126, 702
BccI CCATC 1 cut(s) 388
BciT130I CCWGG 4 cut(s) 146, 243, 421, 759
BfaI CTAG 3 cut(s) 344, 557, 701
BglII AGATCT 1 cut(s) 496
BisI GCNGC 4 cut(s) 140, 551, 691, 795
BlsI GCNGC 4 cut(s) 141, 552, 692, 796
Bme1390I CCNGG 4 cut(s) 146, 243, 421, 759
BmrFI CCNGG 4 cut(s) 146, 243, 421, 759
BmsI GCATC 4 cut(s) 19, 49, 697, 707
BpiI GAAGAC 3 cut(s) 117, 144, 734
BpmI CTGGAG 1 cut(s) 780
BpuEI CTTGAG 1 cut(s) 211
Bsa29I ATCGAT 1 cut(s) 188
BsaBI GATNNNNATC 1 cut(s) 495
BsaJI CCNNGG 3 cut(s) 9, 144, 145
BsaXI ACNNNNNCTCC 2 cut(s) 753, 783
Bsc4I CCNNNNNNNGG 1 cut(s) 737
Bse3DI GCAATG 1 cut(s) 689
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 4 cut(s) 146, 243, 421, 759
BseCI ATCGAT 1 cut(s) 188
BseDI CCNNGG 3 cut(s) 9, 144, 145
BseGI GGATG 1 cut(s) 434
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 1 cut(s) 737
BseMI GCAATG 1 cut(s) 689
BseMII CTCAG 1 cut(s) 375
BseRI GAGGAG 1 cut(s) 542
BseXI GCAGC 2 cut(s) 126, 702
BseYI CCCAGC 1 cut(s) 546
BshVI ATCGAT 1 cut(s) 188
BslI CCNNNNNNNGG 1 cut(s) 737
Bsp143I GATC 3 cut(s) 248, 480, 496
BspACI CCGC 2 cut(s) 32, 551
BspCNI CTCAG 1 cut(s) 376
BspDI ATCGAT 1 cut(s) 188
BspPI GGATC 1 cut(s) 256
BspQI GCTCTTC 2 cut(s) 363, 621
BsrDI GCAATG 1 cut(s) 689
BssECI CCNNGG 3 cut(s) 9, 144, 145
BssMI GATC 3 cut(s) 248, 480, 496
Bst2UI CCWGG 4 cut(s) 146, 243, 421, 759
Bst4CI ACNGT 1 cut(s) 658
Bst6I CTCTTC 2 cut(s) 363, 621
BstDEI CTNAG 1 cut(s) 384
BstDSI CCRYGG 1 cut(s) 9
BstENI CCTNNNNNAGG 1 cut(s) 735
BstF5I GGATG 1 cut(s) 434
BstHHI GCGC 2 cut(s) 321, 722
BstKTI GATC 3 cut(s) 251, 483, 499
BstMBI GATC 3 cut(s) 248, 480, 496
BstNI CCWGG 4 cut(s) 146, 243, 421, 759
BstNSI RCATGY 1 cut(s) 729
BstSCI CCNGG 4 cut(s) 144, 241, 419, 757
BstV1I GCAGC 2 cut(s) 126, 702
BstV2I GAAGAC 3 cut(s) 117, 144, 734
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
Bsu15I ATCGAT 1 cut(s) 188
BsuTUI ATCGAT 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 9
BtgZI GCGATG 1 cut(s) 720
BtsCI GGATG 1 cut(s) 434
CfoI GCGC 2 cut(s) 321, 722
ClaI ATCGAT 1 cut(s) 188
CviAII CATG 1 cut(s) 726
DdeI CTNAG 1 cut(s) 384
DpnI GATC 3 cut(s) 250, 482, 498
DpnII GATC 3 cut(s) 248, 480, 496
DraI TTTAAA 1 cut(s) 736
DrdI GACNNNNNNGTC 1 cut(s) 121
DseDI GACNNNNNNGTC 1 cut(s) 121
Eam1104I CTCTTC 2 cut(s) 363, 621
EarI CTCTTC 2 cut(s) 363, 621
Eco57I CTGAAG 1 cut(s) 642
EcoNI CCTNNNNNAGG 1 cut(s) 735
EcoRII CCWGG 4 cut(s) 144, 241, 419, 757
EcoT22I ATGCAT 1 cut(s) 64
FaeI CATG 1 cut(s) 729
FaiI YATR 2 cut(s) 113, 727
FalI AAGNNNNNCTT 2 cut(s) 577, 609
FatI CATG 1 cut(s) 725
Fnu4HI GCNGC 4 cut(s) 140, 551, 691, 795
FokI GGATG 1 cut(s) 421
Fsp4HI GCNGC 4 cut(s) 140, 551, 691, 795
FspBI CTAG 3 cut(s) 344, 557, 701
GlaI GCGC 2 cut(s) 320, 721
GluI GCNGC 4 cut(s) 140, 551, 691, 795
GsaI CCCAGC 1 cut(s) 550
GsuI CTGGAG 1 cut(s) 780
HhaI GCGC 2 cut(s) 321, 722
Hin1II CATG 1 cut(s) 729
Hin6I GCGC 2 cut(s) 319, 720
HinP1I GCGC 2 cut(s) 319, 720
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 2 cut(s) 275, 491
HphI GGTGA 1 cut(s) 575
Hpy166II GTNNAC 3 cut(s) 163, 467, 698
Hpy188I TCNGA 5 cut(s) 274, 385, 480, 496, 501
Hpy188III TCNNGA 3 cut(s) 235, 344, 362
Hpy8I GTNNAC 3 cut(s) 163, 467, 698
HpyAV CCTTC 3 cut(s) 61, 426, 760
HpyCH4III ACNGT 1 cut(s) 658
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 3 cut(s) 62, 606, 794
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 1 cut(s) 729
HspAI GCGC 2 cut(s) 319, 720
Kzo9I GATC 3 cut(s) 248, 480, 496
LguI GCTCTTC 2 cut(s) 363, 621
LmnI GCTCC 2 cut(s) 70, 83
Lsp1109I GCAGC 2 cut(s) 126, 702
LweI GCATC 4 cut(s) 19, 49, 697, 707
MaeI CTAG 3 cut(s) 344, 557, 701
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 1 cut(s) 324
MalI GATC 3 cut(s) 250, 482, 498
MboI GATC 3 cut(s) 248, 480, 496
MboII GAAGA 9 cut(s) 29, 117, 144, 270, 350, 436, 635, 638, 734
MflI RGATCY 1 cut(s) 496
MluCI AATT 6 cut(s) 108, 194, 258, 373, 601, 670
MmeI TCCRAC 1 cut(s) 556
MnlI CCTC 8 cut(s) 114, 166, 316, 502, 520, 523, 609, 756
Mph1103I ATGCAT 1 cut(s) 64
MseI TTAA 5 cut(s) 131, 446, 521, 536, 735
MspA1I CMGCKG 2 cut(s) 139, 693
MspR9I CCNGG 4 cut(s) 146, 243, 421, 759
MvaI CCWGG 4 cut(s) 146, 243, 421, 759
NdeII GATC 3 cut(s) 248, 480, 496
NlaIII CATG 1 cut(s) 729
NsiI ATGCAT 1 cut(s) 64
NspI RCATGY 1 cut(s) 729
PasI CCCWGGG 1 cut(s) 145
PciI ACATGT 1 cut(s) 725
PciSI GCTCTTC 2 cut(s) 363, 621
PfeI GAWTC 2 cut(s) 275, 491
PfoI TCCNGGA 1 cut(s) 419
PkrI GCNGC 4 cut(s) 141, 552, 692, 796
PscI ACATGT 1 cut(s) 725
Psp6I CCWGG 4 cut(s) 144, 241, 419, 757
PspFI CCCAGC 1 cut(s) 546
PspGI CCWGG 4 cut(s) 144, 241, 419, 757
PsuI RGATCY 1 cut(s) 496
PvuII CAGCTG 2 cut(s) 139, 693
SapI GCTCTTC 2 cut(s) 363, 621
SaqAI TTAA 5 cut(s) 131, 446, 521, 536, 735
SatI GCNGC 4 cut(s) 140, 551, 691, 795
Sau3AI GATC 3 cut(s) 248, 480, 496
ScrFI CCNGG 4 cut(s) 146, 243, 421, 759
SfaNI GCATC 4 cut(s) 19, 49, 697, 707
SmlI CTYRAG 1 cut(s) 226
SmoI CTYRAG 1 cut(s) 226
Sse9I AATT 6 cut(s) 108, 194, 258, 373, 601, 670
SsiI CCGC 2 cut(s) 32, 551
SspMI CTAG 3 cut(s) 344, 557, 701
StyD4I CCNGG 4 cut(s) 144, 241, 419, 757
TaaI ACNGT 1 cut(s) 658
TaiI ACGT 1 cut(s) 168
TaqI TCGA 2 cut(s) 188, 715
TaqII GACCGA 1 cut(s) 131
TasI AATT 6 cut(s) 108, 194, 258, 373, 601, 670
TauI GCSGC 1 cut(s) 553
TfiI GAWTC 2 cut(s) 275, 491
Tru1I TTAA 5 cut(s) 131, 446, 521, 536, 735
Tru9I TTAA 5 cut(s) 131, 446, 521, 536, 735
TseI GCWGC 3 cut(s) 139, 690, 794
TspDTI ATGAA 1 cut(s) 423
XagI CCTNNNNNAGG 1 cut(s) 735
XapI RAATTY 2 cut(s) 194, 373
XbaI TCTAGA 1 cut(s) 343
XceI RCATGY 1 cut(s) 729
XspI CTAG 3 cut(s) 344, 557, 701
Zsp2I ATGCAT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.