Rroxscaffold_5G00386890

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S cluster from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Has anti- apoptotic effects in the cell

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0008748
Physical Location & Seq
Forward (+)
65637348 .. 65638923
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386890.1

Sequence Viewer

Length: 786 bp
ATGCAGGGATCGGTGCTGGCATTTACAGATGATGCGGTTCTACCTGCCAGTCTGGTTTTTGATGCATTAAGGGAGCTTGGGAATGGAGCTTCAGATAAGTGTGAACCTCAAATTATCACTCAAGTGTCTTCCTTGGGGTCAGCTGCCACAGGGGCTTCCTCTATCGACATAGTGTTTGCCCTTTGCAGATCAATTGAATTCCTGAGTGAGCAGTTGTTGAAGGAGACCTCAAGAGTCTTGAAGCCTGGTGGAACAATCCTAGTTTACAAGACTTCTGAAACAGATAAGACCACCTCTGTTCTTGAGCGCAAGTTACTTTTGTCTGGTTTCCTAGAAGCACAAGCTCTTCAAACAAAATCATCTGAGTTGTGCTCTGGAGGTCAAGGCCAAAAGCCTTCTTGGAAAATTGGCTCATCCTTTGCCCTAAAAAAGCCTTCAAAAACTCTACCAAAGTTGCAGATCGATGATGATTCAGATCTGATTGATGAGGATAGTCTATTAACGGAAGAGGACTTGAAGAAACCCCAGCCGGTCGTAGCTGATGATTGTGAAGTTGGAAGCACAAGGAAAGCTTGTAAAAATTGCACTTGTGGGAGGGCTGAAGCGGAGCAAAAAGTAGAGAAGTTACAATCCACTGTGGATTTGAGCAGTTTCCAGTCAGCATGTGGCAGTTGTGGACTAGGGGATGCTTTCCGATGCGCTACATGTCCTTATAAGGGTCTTCCTCCATTCAAACCAGGCGAGAAGGTAGCGTTCTCTTCAAACTTCCTTGCAGCAGACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006790 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016226 GO:0022607 GO:0031163 GO:0044085 GO:0044237 GO:0044424 GO:0044464 GO:0051186 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIAPIN1 Methyltransf_11

Protein Analysis

261

Amino Acids

27.76

Weight (kDa)

4.96

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anamorsin_N PF20922 40 - 120 7.3e-07 Anamorsin, N-terminal
CIAPIN1 PF05093 206 - 251 1.8e-15 Cytokine-induced anti-apoptosis inhibitor 1, Fe-S biogenesis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
Acc36I ACCTGC 1 cut(s) 52
AciI CCGC 2 cut(s) 35, 605
AclWI GGATC 1 cut(s) 16
AcsI RAATTY 1 cut(s) 197
AcuI CTGAAG 2 cut(s) 75, 621
AfiI CCNNNNNNNGG 1 cut(s) 716
AflIII ACRYGT 1 cut(s) 704
AgsI TTSAA 8 cut(s) 197, 220, 241, 350, 438, 517, 733, 762
AjnI CCWGG 2 cut(s) 244, 736
AleI CACNNNNGTG 1 cut(s) 122
AluBI AGCT 6 cut(s) 76, 89, 143, 344, 539, 572
AluI AGCT 6 cut(s) 76, 89, 143, 344, 539, 572
Alw21I GWGCWC 1 cut(s) 374
Alw26I GTCTC 1 cut(s) 218
AlwI GGATC 1 cut(s) 16
AoxI GGCC 1 cut(s) 385
ApeKI GCWGC 2 cut(s) 143, 773
ApoI RAATTY 1 cut(s) 197
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AspLEI GCGC 2 cut(s) 309, 701
BbsI GAAGAC 2 cut(s) 120, 713
Bbv12I GWGCWC 1 cut(s) 374
BbvI GCAGC 1 cut(s) 130
BciT130I CCWGG 2 cut(s) 246, 738
BcoDI GTCTC 1 cut(s) 218
BfaI CTAG 3 cut(s) 260, 332, 680
BfuAI ACCTGC 1 cut(s) 52
BglI GCCNNNNNGGC 1 cut(s) 152
BglII AGATCT 1 cut(s) 475
BisI GCNGC 2 cut(s) 144, 774
BlsI GCNGC 2 cut(s) 145, 775
Bme1390I CCNGG 2 cut(s) 246, 738
BmrFI CCNGG 2 cut(s) 246, 738
BmsI GCATC 4 cut(s) 22, 52, 676, 686
BpiI GAAGAC 2 cut(s) 120, 713
BplI GAGNNNNNCTC 2 cut(s) 356, 388
BpmI CTGGAG 1 cut(s) 396
BpuEI CTTGAG 3 cut(s) 105, 214, 323
Bsa29I ATCGAT 1 cut(s) 462
BsaBI GATNNNNATC 1 cut(s) 474
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 132
Bsc4I CCNNNNNNNGG 1 cut(s) 716
Bse118I RCCGGY 1 cut(s) 529
Bse1I ACTGG 2 cut(s) 48, 655
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 2 cut(s) 246, 738
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 2 cut(s) 413, 691
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 1 cut(s) 716
BseMII CTCAG 2 cut(s) 194, 354
BseNI ACTGG 2 cut(s) 48, 655
BseXI GCAGC 1 cut(s) 130
BseYI CCCAGC 1 cut(s) 525
Bsh1285I CGRYCG 1 cut(s) 534
BshFI GGCC 1 cut(s) 387
BshVI ATCGAT 1 cut(s) 462
BsiEI CGRYCG 1 cut(s) 534
BsiHKAI GWGCWC 1 cut(s) 374
BsiSI CCGG 1 cut(s) 530
BslI CCNNNNNNNGG 1 cut(s) 716
BsmAI GTCTC 1 cut(s) 218
BsnI GGCC 1 cut(s) 387
Bso31I GGTCTC 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 374
Bsp143I GATC 4 cut(s) 8, 188, 459, 475
BspACI CCGC 2 cut(s) 35, 605
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 2 cut(s) 195, 355
BspDI ATCGAT 1 cut(s) 462
BspMI ACCTGC 1 cut(s) 52
BspPI GGATC 1 cut(s) 16
BspQI GCTCTTC 1 cut(s) 351
BspTNI GGTCTC 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 529
BsrI ACTGG 2 cut(s) 48, 655
BssAI RCCGGY 1 cut(s) 529
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 4 cut(s) 8, 188, 459, 475
BssT1I CCWWGG 1 cut(s) 132
Bst2UI CCWGG 2 cut(s) 246, 738
Bst4CI ACNGT 1 cut(s) 637
Bst6I CTCTTC 3 cut(s) 351, 501, 763
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 2 cut(s) 203, 363
BstF5I GGATG 2 cut(s) 413, 691
BstHHI GCGC 2 cut(s) 309, 701
BstKTI GATC 4 cut(s) 11, 191, 462, 478
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 4 cut(s) 8, 188, 459, 475
BstMCI CGRYCG 1 cut(s) 534
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstNI CCWGG 2 cut(s) 246, 738
BstNSI RCATGY 2 cut(s) 666, 708
BstSCI CCNGG 2 cut(s) 244, 736
BstV1I GCAGC 1 cut(s) 130
BstV2I GAAGAC 2 cut(s) 120, 713
BstX2I RGATCY 1 cut(s) 475
BstYI RGATCY 1 cut(s) 475
Bsu15I ATCGAT 1 cut(s) 462
BsuRI GGCC 1 cut(s) 387
BsuTUI ATCGAT 1 cut(s) 462
BtsCI GGATG 2 cut(s) 413, 691
BtsIMutI CAGTG 1 cut(s) 633
BveI ACCTGC 1 cut(s) 52
Cac8I GCNNGC 1 cut(s) 18
CfoI GCGC 2 cut(s) 309, 701
Cfr10I RCCGGY 1 cut(s) 529
ClaI ATCGAT 1 cut(s) 462
CviAII CATG 2 cut(s) 663, 705
DdeI CTNAG 2 cut(s) 203, 363
DpnI GATC 4 cut(s) 10, 190, 461, 477
DpnII GATC 4 cut(s) 8, 188, 459, 475
Eam1104I CTCTTC 3 cut(s) 351, 501, 763
EarI CTCTTC 3 cut(s) 351, 501, 763
Eco130I CCWWGG 1 cut(s) 132
Eco31I GGTCTC 1 cut(s) 218
Eco57I CTGAAG 2 cut(s) 75, 621
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 2 cut(s) 244, 736
EcoT14I CCWWGG 1 cut(s) 132
EcoT22I ATGCAT 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 2 cut(s) 666, 708
FaiI YATR 6 cut(s) 170, 664, 706, 714, 782, 784
FalI AAGNNNNNCTT 2 cut(s) 556, 588
FatI CATG 2 cut(s) 662, 704
Fnu4HI GCNGC 2 cut(s) 144, 774
FokI GGATG 2 cut(s) 400, 698
Fsp4HI GCNGC 2 cut(s) 144, 774
FspBI CTAG 3 cut(s) 260, 332, 680
GlaI GCGC 2 cut(s) 308, 700
GluI GCNGC 2 cut(s) 144, 774
GsaI CCCAGC 1 cut(s) 529
GsuI CTGGAG 1 cut(s) 396
HaeIII GGCC 1 cut(s) 387
HapII CCGG 1 cut(s) 530
HhaI GCGC 2 cut(s) 309, 701
Hin1II CATG 2 cut(s) 666, 708
Hin6I GCGC 2 cut(s) 307, 699
HinP1I GCGC 2 cut(s) 307, 699
HindIII AAGCTT 1 cut(s) 570
HinfI GANTC 2 cut(s) 234, 470
HpaII CCGG 1 cut(s) 530
Hpy166II GTNNAC 3 cut(s) 104, 265, 677
Hpy188I TCNGA 6 cut(s) 94, 277, 364, 475, 480, 695
Hpy188III TCNNGA 5 cut(s) 202, 231, 238, 302, 375
Hpy8I GTNNAC 3 cut(s) 104, 265, 677
HpyAV CCTTC 4 cut(s) 214, 405, 444, 739
HpyCH4III ACNGT 1 cut(s) 637
HpyCH4V TGCA 6 cut(s) 4, 65, 186, 457, 585, 773
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpyF3I CTNAG 2 cut(s) 203, 363
Hsp92II CATG 2 cut(s) 666, 708
HspAI GCGC 2 cut(s) 307, 699
Kzo9I GATC 4 cut(s) 8, 188, 459, 475
LguI GCTCTTC 1 cut(s) 351
LmnI GCTCC 3 cut(s) 73, 86, 607
Lsp1109I GCAGC 1 cut(s) 130
LweI GCATC 4 cut(s) 22, 52, 676, 686
MaeI CTAG 3 cut(s) 260, 332, 680
MaeIII GTNAC 2 cut(s) 312, 624
MalI GATC 4 cut(s) 10, 190, 461, 477
MboI GATC 4 cut(s) 8, 188, 459, 475
MboII GAAGA 6 cut(s) 120, 338, 518, 529, 713, 750
MfeI CAATTG 1 cut(s) 192
MflI RGATCY 1 cut(s) 475
MhlI GDGCHC 1 cut(s) 374
MluCI AATT 5 cut(s) 111, 192, 197, 405, 580
MlyI GAGTC 1 cut(s) 243
MmeI TCCRAC 1 cut(s) 535
MnlI CCTC 9 cut(s) 117, 169, 238, 304, 371, 481, 502, 588, 735
Mph1103I ATGCAT 1 cut(s) 67
MseI TTAA 2 cut(s) 68, 500
MslI CAYNNNNRTG 1 cut(s) 122
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 1 cut(s) 530
MspR9I CCNGG 2 cut(s) 246, 738
MunI CAATTG 1 cut(s) 192
MvaI CCWGG 2 cut(s) 246, 738
MwoI GCNNNNNNNGC 1 cut(s) 152
NdeII GATC 4 cut(s) 8, 188, 459, 475
NlaIII CATG 2 cut(s) 666, 708
NsiI ATGCAT 1 cut(s) 67
NspI RCATGY 2 cut(s) 666, 708
OliI CACNNNNGTG 1 cut(s) 122
PciI ACATGT 1 cut(s) 704
PciSI GCTCTTC 1 cut(s) 351
PfeI GAWTC 1 cut(s) 470
PkrI GCNGC 2 cut(s) 145, 775
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
PscI ACATGT 1 cut(s) 704
PsiI TTATAA 1 cut(s) 714
Psp6I CCWGG 2 cut(s) 244, 736
PspFI CCCAGC 1 cut(s) 525
PspGI CCWGG 2 cut(s) 244, 736
PsuI RGATCY 1 cut(s) 475
PvuII CAGCTG 1 cut(s) 143
RseI CAYNNNNRTG 1 cut(s) 122
SapI GCTCTTC 1 cut(s) 351
SaqAI TTAA 2 cut(s) 68, 500
SatI GCNGC 2 cut(s) 144, 774
Sau3AI GATC 4 cut(s) 8, 188, 459, 475
SchI GAGTC 1 cut(s) 243
ScrFI CCNGG 2 cut(s) 246, 738
SduI GDGCHC 1 cut(s) 374
SfaNI GCATC 4 cut(s) 22, 52, 676, 686
SmiMI CAYNNNNRTG 1 cut(s) 122
SmlI CTYRAG 3 cut(s) 120, 229, 302
SmoI CTYRAG 3 cut(s) 120, 229, 302
Sse9I AATT 5 cut(s) 111, 192, 197, 405, 580
SsiI CCGC 2 cut(s) 35, 605
SspMI CTAG 3 cut(s) 260, 332, 680
StyD4I CCNGG 2 cut(s) 244, 736
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 1 cut(s) 637
TaqI TCGA 2 cut(s) 165, 462
TasI AATT 5 cut(s) 111, 192, 197, 405, 580
TfiI GAWTC 1 cut(s) 470
Tru1I TTAA 2 cut(s) 68, 500
Tru9I TTAA 2 cut(s) 68, 500
TscAI CASTG 1 cut(s) 640
TseI GCWGC 2 cut(s) 143, 773
TspGWI ACGGA 1 cut(s) 518
TspRI CASTG 1 cut(s) 640
XapI RAATTY 1 cut(s) 197
XceI RCATGY 2 cut(s) 666, 708
XcmI CCANNNNNNNNNTGG 1 cut(s) 662
XspI CTAG 3 cut(s) 260, 332, 680
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.