AT5G18430

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007620
Physical Location & Seq
Reverse (-)
6110151 .. 6112039
1889 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G18430.1

Sequence Viewer

Length: 1089 bp
ATGACGATATCAACGGTTATAGCATTCATGTCCATGTTCCTAGTATTTGTTATGTCTGGTCCGATAGTGGTGGAGGGACGTGCTTTTTTCGTGTTCGGTGACTCGCTCGTCGACAGTGGCAACAACAACTATCTGGTGACAACTGCACGTGCCGACTCTCCACCGTATGGAATCGATTTTCCAACTCGGAGACCAACCGGCCGGTTTTCCAACGGTCTTAACATTCCAGATCTTATCAGTGAGGCAATCGGTAACGAAGAGCCACCGTTACCTTACCTTAGTCCAGAGCTAAGGGGCCGGAGTCTTCTTAACGGTGCTAATTTCGCCTCCGCTGGTATCGGAATTCTCAATGATACCGGATTTCAATTCATCAACATTATAAGAATGTACCAACAACTCGATTACTTCCAACAATACCAACAACGTGTGAGTCGCTTGATTGGGAAACCGCAAACTCAGCGGCTCGTGAGTCAAGCGCTTGTATTAATCACGGTCGGTGGAAACGATTTTGTGAACAACTATTTCTTGTTTCCATATTCCGCTAGATCACGCCAATTCACTCTACCGGATTACGTCCGACTCCTAATTTCTGAATACAAAAAAATACTATTGAGGTTAAATTCACTTGGAGTGGGTCGAGTTCTAGTGACAGGAGCTGGACCACTAGGATGTGCACCGGCCGAACTGGCGAGATCAGGTACGTCGAATGGAAGATGCTCGGCCGAGCTACAACGAGCTGCATCTCTATATGATCCTCAGCTACTTCAAATGATAAATGAACTTAACAAAAAAATTGGGAGAAATGTGTTTATTGCTGCTAACACTAACCAAATGCAAGAGGATTTTCTAAGCACTCCACGAAGATATGGATTTGTAACATCGAAGGTTGCTTGTTGCGGACAAGGGCCGTACAATGGGATGGGTTTGTGTACGGTTCTATCAAACTTGTGTCCGAACCGTGAGTTATACGTCTTTTGGGATGCGTTTCATCCGACTGAGAAAGCAAACCGCATGATAGTCCGACATATCTTGACCGGTACCACCAAGTATATGAACCCCATGAACCTTAGCTCTGCTCTTGCCCTATGA

Protein Analysis

362

Amino Acids

40.12

Weight (kDa)

9.31

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 343 8.1e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 380
AasI GACNNNNNNGTC 1 cut(s) 107
Acc65I GGTACC 1 cut(s) 1037
AccB1I GGYRCC 1 cut(s) 1037
AccB7I CCANNNNNTGG 1 cut(s) 167
AccI GTMKAC 1 cut(s) 111
AciI CCGC 6 cut(s) 330, 449, 460, 540, 897, 1009
AclWI GGATC 1 cut(s) 746
AcoI YGGCCR 3 cut(s) 199, 678, 720
AcsI RAATTY 2 cut(s) 342, 619
AcvI CACGTG 1 cut(s) 149
AfaI GTAC 5 cut(s) 389, 700, 911, 931, 1039
AfeI AGCGCT 1 cut(s) 477
AfiI CCNNNNNNNGG 2 cut(s) 167, 914
AflIII ACRYGT 1 cut(s) 424
AgeI ACCGGT 1 cut(s) 1034
AgsI TTSAA 2 cut(s) 365, 767
AjiI CACGTC 1 cut(s) 80
AluBI AGCT 6 cut(s) 289, 656, 727, 737, 760, 1071
AluI AGCT 6 cut(s) 289, 656, 727, 737, 760, 1071
Alw21I GWGCWC 1 cut(s) 676
Alw26I GTCTC 1 cut(s) 184
Alw44I GTGCAC 1 cut(s) 672
AlwI GGATC 1 cut(s) 746
AlwNI CAGNNNCTG 1 cut(s) 656
Aor51HI AGCGCT 1 cut(s) 477
AoxI GGCC 5 cut(s) 199, 295, 678, 720, 905
ApaLI GTGCAC 1 cut(s) 672
ApeKI GCWGC 2 cut(s) 737, 815
ApoI RAATTY 2 cut(s) 342, 619
AseI ATTAAT 1 cut(s) 485
AsiGI ACCGGT 1 cut(s) 1034
Asp718I GGTACC 1 cut(s) 1037
AspLEI GCGC 1 cut(s) 478
AspS9I GGNCC 4 cut(s) 59, 295, 659, 905
AsuHPI GGTGA 2 cut(s) 110, 148
AvaII GGWCC 2 cut(s) 59, 659
BaeGI GKGCMC 1 cut(s) 676
BanI GGYRCC 1 cut(s) 1037
BauI CACGAG 1 cut(s) 464
BbrPI CACGTG 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 676
BbvCI CCTCAGC 1 cut(s) 756
BbvI GCAGC 2 cut(s) 724, 802
BccI CCATC 1 cut(s) 913
BceAI ACGGC 1 cut(s) 892
BcoDI GTCTC 1 cut(s) 184
BfaI CTAG 4 cut(s) 41, 543, 644, 665
BfoI RGCGCY 1 cut(s) 479
BglI GCCNNNNNGGC 1 cut(s) 686
BglII AGATCT 1 cut(s) 229
BisI GCNGC 3 cut(s) 461, 738, 816
BlsI GCNGC 3 cut(s) 462, 739, 817
Bme18I GGWCC 2 cut(s) 59, 659
BmgBI CACGTC 1 cut(s) 80
BmgT120I GGNCC 4 cut(s) 59, 295, 659, 905
BmiI GGNNCC 2 cut(s) 296, 1039
BmsI GCATC 3 cut(s) 704, 749, 970
BpiI GAAGAC 1 cut(s) 296
Bpu10I CCTNAGC 3 cut(s) 290, 756, 1067
Bsa29I ATCGAT 1 cut(s) 174
BsaAI YACGTR 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 184
BsaWI WCCGGW 3 cut(s) 356, 565, 1034
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 914
Bse118I RCCGGY 4 cut(s) 197, 201, 676, 1034
Bse1I ACTGG 1 cut(s) 690
BseCI ATCGAT 1 cut(s) 174
BseGI GGATG 4 cut(s) 674, 924, 985, 988
BseLI CCNNNNNNNGG 2 cut(s) 167, 914
BseMII CTCAG 3 cut(s) 470, 770, 987
BseNI ACTGG 1 cut(s) 690
BseSI GKGCMC 1 cut(s) 676
BseX3I CGGCCG 3 cut(s) 199, 678, 720
BseXI GCAGC 2 cut(s) 724, 802
BsgI GTGCAG 1 cut(s) 129
Bsh1285I CGRYCG 4 cut(s) 202, 495, 681, 723
BshFI GGCC 5 cut(s) 201, 297, 680, 722, 907
BshNI GGYRCC 1 cut(s) 1037
BshTI ACCGGT 1 cut(s) 1034
BshVI ATCGAT 1 cut(s) 174
BsiEI CGRYCG 4 cut(s) 202, 495, 681, 723
BsiHKAI GWGCWC 1 cut(s) 676
BsiSI CCGG 7 cut(s) 198, 202, 298, 357, 566, 677, 1035
BslFI GGGAC 1 cut(s) 90
BslI CCNNNNNNNGG 2 cut(s) 167, 914
BsmAI GTCTC 1 cut(s) 184
BsmFI GGGAC 1 cut(s) 90
BsmI GAATGC 1 cut(s) 23
BsnI GGCC 5 cut(s) 201, 297, 680, 722, 907
Bso31I GGTCTC 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 676
Bsp143I GATC 4 cut(s) 229, 545, 692, 751
BspACI CCGC 6 cut(s) 330, 449, 460, 540, 897, 1009
BspANI GGCC 5 cut(s) 201, 297, 680, 722, 907
BspCNI CTCAG 3 cut(s) 469, 769, 988
BspDI ATCGAT 1 cut(s) 174
BspLI GGNNCC 2 cut(s) 296, 1039
BspPI GGATC 1 cut(s) 746
BspQI GCTCTTC 1 cut(s) 252
BspT107I GGYRCC 1 cut(s) 1037
BspTNI GGTCTC 1 cut(s) 184
BsrFI RCCGGY 4 cut(s) 197, 201, 676, 1034
BsrI ACTGG 1 cut(s) 690
BssAI RCCGGY 4 cut(s) 197, 201, 676, 1034
BssMI GATC 4 cut(s) 229, 545, 692, 751
BssSI CACGAG 1 cut(s) 464
Bst2BI CACGAG 1 cut(s) 464
Bst4CI ACNGT 9 cut(s) 16, 116, 165, 215, 267, 314, 493, 934, 959
Bst6I CTCTTC 1 cut(s) 252
BstBAI YACGTR 1 cut(s) 149
BstDEI CTNAG 7 cut(s) 278, 290, 456, 756, 848, 996, 1067
BstF5I GGATG 4 cut(s) 674, 924, 985, 988
BstH2I RGCGCY 1 cut(s) 479
BstHHI GCGC 1 cut(s) 478
BstKTI GATC 4 cut(s) 232, 548, 695, 754
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 4 cut(s) 229, 545, 692, 751
BstMCI CGRYCG 4 cut(s) 202, 495, 681, 723
BstMWI GCNNNNNNNGC 3 cut(s) 323, 457, 686
BstSLI GKGCMC 1 cut(s) 676
BstV1I GCAGC 2 cut(s) 724, 802
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BstZI CGGCCG 3 cut(s) 199, 678, 720
Bsu15I ATCGAT 1 cut(s) 174
BsuRI GGCC 5 cut(s) 201, 297, 680, 722, 907
BsuTUI ATCGAT 1 cut(s) 174
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 4 cut(s) 674, 924, 985, 988
BtsIMutI CAGTG 2 cut(s) 121, 244
CaiI CAGNNNCTG 1 cut(s) 656
CfoI GCGC 1 cut(s) 478
Cfr10I RCCGGY 4 cut(s) 197, 201, 676, 1034
Cfr13I GGNCC 4 cut(s) 59, 295, 659, 905
ClaI ATCGAT 1 cut(s) 174
Csp6I GTAC 5 cut(s) 388, 699, 910, 930, 1038
CspAI ACCGGT 1 cut(s) 1034
CviAII CATG 4 cut(s) 28, 34, 1012, 1060
CviQI GTAC 5 cut(s) 388, 699, 910, 930, 1038
DdeI CTNAG 7 cut(s) 278, 290, 456, 756, 848, 996, 1067
DpnI GATC 4 cut(s) 231, 547, 694, 753
DpnII GATC 4 cut(s) 229, 545, 692, 751
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 3 cut(s) 199, 678, 720
EagI CGGCCG 3 cut(s) 199, 678, 720
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
EclXI CGGCCG 3 cut(s) 199, 678, 720
Eco31I GGTCTC 1 cut(s) 184
Eco32I GATATC 1 cut(s) 9
Eco47I GGWCC 2 cut(s) 59, 659
Eco47III AGCGCT 1 cut(s) 477
Eco52I CGGCCG 3 cut(s) 199, 678, 720
Eco72I CACGTG 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 342
EcoRV GATATC 1 cut(s) 9
FaeI CATG 4 cut(s) 31, 37, 1015, 1063
FaqI GGGAC 1 cut(s) 90
FatI CATG 4 cut(s) 27, 33, 1011, 1059
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 3 cut(s) 461, 738, 816
FokI GGATG 4 cut(s) 681, 931, 975, 992
Fsp4HI GCNGC 3 cut(s) 461, 738, 816
FspBI CTAG 4 cut(s) 41, 543, 644, 665
GlaI GCGC 1 cut(s) 477
GluI GCNGC 3 cut(s) 461, 738, 816
HaeII RGCGCY 1 cut(s) 479
HaeIII GGCC 5 cut(s) 201, 297, 680, 722, 907
HapII CCGG 7 cut(s) 198, 202, 298, 357, 566, 677, 1035
HhaI GCGC 1 cut(s) 478
Hin1II CATG 4 cut(s) 31, 37, 1015, 1063
Hin6I GCGC 1 cut(s) 476
HinP1I GCGC 1 cut(s) 476
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HinfI GANTC 7 cut(s) 101, 155, 171, 301, 430, 469, 579
HpaII CCGG 7 cut(s) 198, 202, 298, 357, 566, 677, 1035
HphI GGTGA 2 cut(s) 110, 148
Hpy166II GTNNAC 4 cut(s) 112, 514, 674, 930
Hpy188I TCNGA 8 cut(s) 63, 189, 341, 578, 592, 954, 993, 1022
Hpy188III TCNNGA 4 cut(s) 227, 284, 466, 1030
Hpy8I GTNNAC 4 cut(s) 112, 514, 674, 930
Hpy99I CGWCG 2 cut(s) 113, 706
HpyAV CCTTC 1 cut(s) 877
HpyCH4III ACNGT 9 cut(s) 16, 116, 165, 215, 267, 314, 493, 934, 959
HpyCH4IV ACGT 6 cut(s) 79, 148, 424, 573, 701, 969
HpyCH4V TGCA 4 cut(s) 146, 674, 740, 835
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 457, 686
HpyF3I CTNAG 7 cut(s) 278, 290, 456, 756, 848, 996, 1067
HpySE526I ACGT 6 cut(s) 79, 148, 424, 573, 701, 969
Hsp92II CATG 4 cut(s) 31, 37, 1015, 1063
HspAI GCGC 1 cut(s) 476
KpnI GGTACC 1 cut(s) 1041
Kzo9I GATC 4 cut(s) 229, 545, 692, 751
LguI GCTCTTC 1 cut(s) 252
LmnI GCTCC 1 cut(s) 653
Lsp1109I GCAGC 2 cut(s) 724, 802
LweI GCATC 3 cut(s) 704, 749, 970
MaeI CTAG 4 cut(s) 41, 543, 644, 665
MaeII ACGT 6 cut(s) 79, 148, 424, 573, 701, 969
MaeIII GTNAC 6 cut(s) 98, 136, 251, 267, 646, 874
MalI GATC 4 cut(s) 231, 547, 694, 753
MboI GATC 4 cut(s) 229, 545, 692, 751
MboII GAAGA 4 cut(s) 269, 296, 723, 873
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 676
MluCI AATT 7 cut(s) 319, 342, 365, 554, 585, 619, 792
MlyI GAGTC 6 cut(s) 95, 149, 310, 439, 478, 573
MmeI TCCRAC 6 cut(s) 206, 234, 433, 601, 1016, 1045
MnlI CCTC 6 cut(s) 67, 235, 337, 606, 765, 832
MseI TTAA 5 cut(s) 219, 309, 485, 617, 783
MslI CAYNNNNRTG 2 cut(s) 32, 667
MspA1I CMGCKG 2 cut(s) 332, 460
MspI CCGG 7 cut(s) 198, 202, 298, 357, 566, 677, 1035
Mva1269I GAATGC 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 323, 457, 686
NdeII GATC 4 cut(s) 229, 545, 692, 751
NlaIII CATG 4 cut(s) 31, 37, 1015, 1063
NlaIV GGNNCC 2 cut(s) 296, 1039
NmeAIII GCCGAG 2 cut(s) 698, 748
NmuCI GTSAC 3 cut(s) 98, 136, 646
PciSI GCTCTTC 1 cut(s) 252
PcsI WCGNNNNNNNCGW 1 cut(s) 501
PctI GAATGC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 171
PflMI CCANNNNNTGG 1 cut(s) 167
PinAI ACCGGT 1 cut(s) 1034
PkrI GCNGC 3 cut(s) 462, 739, 817
PleI GAGTC 6 cut(s) 95, 149, 309, 438, 477, 573
PmaCI CACGTG 1 cut(s) 149
PmlI CACGTG 1 cut(s) 149
PpsI GAGTC 6 cut(s) 95, 149, 309, 438, 477, 573
Ppu21I YACGTR 1 cut(s) 149
PshBI ATTAAT 1 cut(s) 485
PsiI TTATAA 1 cut(s) 380
PspCI CACGTG 1 cut(s) 149
PspN4I GGNNCC 2 cut(s) 296, 1039
PspPI GGNCC 4 cut(s) 59, 295, 659, 905
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 229
RsaI GTAC 5 cut(s) 389, 700, 911, 931, 1039
RsaNI GTAC 5 cut(s) 388, 699, 910, 930, 1038
RseI CAYNNNNRTG 2 cut(s) 32, 667
SalI GTCGAC 1 cut(s) 110
SapI GCTCTTC 1 cut(s) 252
SaqAI TTAA 5 cut(s) 219, 309, 485, 617, 783
SatI GCNGC 3 cut(s) 461, 738, 816
Sau3AI GATC 4 cut(s) 229, 545, 692, 751
Sau96I GGNCC 4 cut(s) 59, 295, 659, 905
SchI GAGTC 6 cut(s) 95, 149, 310, 439, 478, 573
SduI GDGCHC 1 cut(s) 676
SfaNI GCATC 3 cut(s) 704, 749, 970
SinI GGWCC 2 cut(s) 59, 659
SmiMI CAYNNNNRTG 2 cut(s) 32, 667
Sse9I AATT 7 cut(s) 319, 342, 365, 554, 585, 619, 792
SsiI CCGC 6 cut(s) 330, 449, 460, 540, 897, 1009
SspMI CTAG 4 cut(s) 41, 543, 644, 665
TaaI ACNGT 9 cut(s) 16, 116, 165, 215, 267, 314, 493, 934, 959
TaiI ACGT 6 cut(s) 82, 151, 427, 576, 704, 972
TaqI TCGA 6 cut(s) 111, 174, 399, 637, 704, 881
TasI AATT 7 cut(s) 319, 342, 365, 554, 585, 619, 792
TauI GCSGC 1 cut(s) 463
TfiI GAWTC 1 cut(s) 171
Tru1I TTAA 5 cut(s) 219, 309, 485, 617, 783
Tru9I TTAA 5 cut(s) 219, 309, 485, 617, 783
TscAI CASTG 2 cut(s) 121, 244
TseFI GTSAC 3 cut(s) 98, 136, 646
TseI GCWGC 2 cut(s) 737, 815
Tsp45I GTSAC 3 cut(s) 98, 136, 646
TspDTI ATGAA 6 cut(s) 16, 358, 792, 977, 1067, 1076
TspRI CASTG 2 cut(s) 121, 244
Van91I CCANNNNNTGG 1 cut(s) 167
VneI GTGCAC 1 cut(s) 672
VpaK11BI GGWCC 2 cut(s) 59, 659
VspI ATTAAT 1 cut(s) 485
XapI RAATTY 2 cut(s) 342, 619
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 4 cut(s) 41, 543, 644, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.