Rorug02G0617300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007620
Physical Location & Seq
Forward (+)
73552627 .. 73553181
555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0617300.1

Sequence Viewer

Length: 555 bp
ATGCTTGCTAGGATTGGTTGGCGGATTATTCAAAATGATAATGGTTTATGGTGCAAAATCTTCTCAGAAAAATATTTGAAGGGGAATTTCCTTGATCATGATTATACAATGCCATCTAGGTATTCTAGCACCTGGTCTAGTGTATGCTTTGGTGCTGCCTTATTAAAAAAAGGTCTTACTTGGAGAATTGGAAATGGAAATTCTGTTTCTTTTTGGACTGATAACTGGACTGAGTTTGGTGCTCTTAAAGATCTTGCGTTGGACTCTACAATGGTCGGTGATAGCATGCTTGTCCAAGATTTTTGGAGTAACAATGAATGGGATGCAACTCTTCTTTATGTTTGTCTGCCAGCTGAGGTAGTGGATAAAATTTTATGTATTCCCATTGATGTTACTGGACAACAATGTGATAAGCTGATTTGGCGACACACTTCTAAAGGCAAGCTCACTGTTAAAAGTGCTTATATTAGCCTTGCCTCAGTTTACACCAACTCCCACCCCCCCCAAGTTAAAGACTTTTACTTGGTTATTTGTCCAAAGTCGCCTTCTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

184

Amino Acids

20.92

Weight (kDa)

7.57

Isoelectric Point (pI)

16.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 22
AcsI RAATTY 3 cut(s) 85, 199, 369
AgsI TTSAA 2 cut(s) 32, 79
AjnI CCWGG 1 cut(s) 131
AjuI GAANNNNNNNTTGG 1 cut(s) 529
AluBI AGCT 3 cut(s) 353, 415, 445
AluI AGCT 3 cut(s) 353, 415, 445
Alw21I GWGCWC 1 cut(s) 244
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 3 cut(s) 85, 199, 369
AsuHPI GGTGA 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 244
BbvCI CCTCAGC 1 cut(s) 354
BbvI GCAGC 1 cut(s) 142
BccI CCATC 1 cut(s) 121
BciT130I CCWGG 1 cut(s) 133
BclI TGATCA 1 cut(s) 94
BfaI CTAG 4 cut(s) 9, 117, 126, 138
BglII AGATCT 1 cut(s) 250
BisI GCNGC 1 cut(s) 156
BlsI GCNGC 1 cut(s) 157
Bme1390I CCNGG 1 cut(s) 133
BmrFI CCNGG 1 cut(s) 133
BmsI GCATC 1 cut(s) 313
Bpu10I CCTNAGC 1 cut(s) 354
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bse1I ACTGG 2 cut(s) 230, 400
BseBI CCWGG 1 cut(s) 133
BseGI GGATG 1 cut(s) 328
BseMII CTCAG 4 cut(s) 78, 222, 345, 492
BseNI ACTGG 2 cut(s) 230, 400
BseXI GCAGC 1 cut(s) 142
BsiHKAI GWGCWC 1 cut(s) 244
Bsp1286I GDGCHC 1 cut(s) 244
Bsp143I GATC 2 cut(s) 94, 250
BspACI CCGC 1 cut(s) 22
BspCNI CTCAG 4 cut(s) 77, 223, 346, 491
BspHI TCATGA 1 cut(s) 97
BsrI ACTGG 2 cut(s) 230, 400
BssMI GATC 2 cut(s) 94, 250
Bst2UI CCWGG 1 cut(s) 133
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 1 cut(s) 336
BstC8I GCNNGC 4 cut(s) 6, 287, 351, 443
BstDEI CTNAG 4 cut(s) 64, 231, 354, 478
BstF5I GGATG 1 cut(s) 328
BstKTI GATC 2 cut(s) 97, 253
BstMBI GATC 2 cut(s) 94, 250
BstMWI GCNNNNNNNGC 1 cut(s) 421
BstNI CCWGG 1 cut(s) 133
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 1 cut(s) 131
BstV1I GCAGC 1 cut(s) 142
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BtsCI GGATG 1 cut(s) 328
BtsIMutI CAGTG 1 cut(s) 447
Cac8I GCNNGC 4 cut(s) 6, 287, 351, 443
CciI TCATGA 1 cut(s) 97
CsiI ACCWGGT 1 cut(s) 131
CviAII CATG 2 cut(s) 98, 286
CviJI RGCY 4 cut(s) 353, 415, 445, 471
CviKI_1 RGCY 4 cut(s) 353, 415, 445, 471
DdeI CTNAG 4 cut(s) 64, 231, 354, 478
DpnI GATC 2 cut(s) 96, 252
DpnII GATC 2 cut(s) 94, 250
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
EciI GGCGGA 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 131
FaeI CATG 2 cut(s) 101, 289
FaiI YATR 8 cut(s) 49, 99, 105, 145, 287, 339, 376, 465
FatI CATG 2 cut(s) 97, 285
FbaI TGATCA 1 cut(s) 94
Fnu4HI GCNGC 1 cut(s) 156
FokI GGATG 1 cut(s) 335
Fsp4HI GCNGC 1 cut(s) 156
FspBI CTAG 4 cut(s) 9, 117, 126, 138
GluI GCNGC 1 cut(s) 156
Hin1II CATG 2 cut(s) 101, 289
HinfI GANTC 1 cut(s) 263
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 1 cut(s) 98
Hpy8I GTNNAC 1 cut(s) 484
HpyAV CCTTC 2 cut(s) 73, 555
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4V TGCA 2 cut(s) 54, 326
HpyF10VI GCNNNNNNNGC 1 cut(s) 421
HpyF3I CTNAG 4 cut(s) 64, 231, 354, 478
Hsp92II CATG 2 cut(s) 101, 289
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 2 cut(s) 94, 250
LpnPI CCDG 5 cut(s) 118, 145, 211, 363, 381
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 1 cut(s) 313
MabI ACCWGGT 1 cut(s) 131
MaeI CTAG 4 cut(s) 9, 117, 126, 138
MaeIII GTNAC 2 cut(s) 308, 391
MalI GATC 2 cut(s) 96, 252
MboI GATC 2 cut(s) 94, 250
MboII GAAGA 2 cut(s) 52, 323
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 1 cut(s) 244
MluCI AATT 4 cut(s) 85, 186, 199, 369
MlyI GAGTC 1 cut(s) 257
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 2 cut(s) 349, 487
MseI TTAA 4 cut(s) 164, 246, 453, 510
MspA1I CMGCKG 1 cut(s) 353
MspR9I CCNGG 1 cut(s) 133
MvaI CCWGG 1 cut(s) 133
MwoI GCNNNNNNNGC 1 cut(s) 421
NdeII GATC 2 cut(s) 94, 250
NlaIII CATG 2 cut(s) 101, 289
NspI RCATGY 1 cut(s) 289
PaeI GCATGC 1 cut(s) 289
PagI TCATGA 1 cut(s) 97
PkrI GCNGC 1 cut(s) 157
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 131
PspGI CCWGG 1 cut(s) 131
PsuI RGATCY 1 cut(s) 250
PvuII CAGCTG 1 cut(s) 353
SaqAI TTAA 4 cut(s) 164, 246, 453, 510
SatI GCNGC 1 cut(s) 156
Sau3AI GATC 2 cut(s) 94, 250
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 1 cut(s) 133
SduI GDGCHC 1 cut(s) 244
SetI ASST 7 cut(s) 122, 134, 175, 355, 360, 417, 447
SexAI ACCWGGT 1 cut(s) 131
SfaNI GCATC 1 cut(s) 313
SphI GCATGC 1 cut(s) 289
Sse9I AATT 4 cut(s) 85, 186, 199, 369
SsiI CCGC 1 cut(s) 22
SspI AATATT 1 cut(s) 74
SspMI CTAG 4 cut(s) 9, 117, 126, 138
StyD4I CCNGG 1 cut(s) 131
TaaI ACNGT 1 cut(s) 451
TasI AATT 4 cut(s) 85, 186, 199, 369
Tru1I TTAA 4 cut(s) 164, 246, 453, 510
Tru9I TTAA 4 cut(s) 164, 246, 453, 510
TscAI CASTG 1 cut(s) 454
TseI GCWGC 1 cut(s) 155
TspDTI ATGAA 1 cut(s) 330
TspRI CASTG 1 cut(s) 454
XapI RAATTY 3 cut(s) 85, 199, 369
XceI RCATGY 1 cut(s) 289
XspI CTAG 4 cut(s) 9, 117, 126, 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.