Rw3G001180

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0007620
Physical Location & Seq
Reverse (-)
950629 .. 952418
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G001180.1

Sequence Viewer

Length: 1041 bp
ATGGCTAGCTCACTGGTGCTGATTTTCAGTCTGGTCCTTACTGCATTGGAAAGCTTCGGCACCCAGCAGGTCGAGGCTCGTGCCTTTTTCGCTCGTGCCGACGCTTATCCTTATGGCATTGACTATCCAACTGGTCGACCAACGGGCCGTTTCTCCAATGGCTACAACATCCCTGACTTCATCAGTCAAGCACTTGGTGCAGAATCCACGTTGCCTTACTTGAGTCCAGAGCTCACTGGACAAAGGCTACTTGTTGGTGCCAACTTTGCTTCTGCTGGCATTGGAATCCTCAATGACACCGGAATTCAGTTTGTAAACATAATCAGAATGTACAGACAATTTGAGTATTTTCAAGAATACCAGCAAAGAGTGAGTGCCCTTATCGGATCTGAACGGGCGGAGCAACTTGTAAACGGAGCACTTATTCTCATGACTGTTGGCGGCAACGACTTTGTCAACAATTACTACTTGGTTCCCTACTCCGCTAGATCTCGTCAATATTCTCTTCCAGATTATGTCAGATACCTCATTTCTGAGTTCAGAAAACTTCTTTTGAGGCTATACGATCTTGGAGGGCGTAGGGTTTTGGTAACAGGTACAGGACCTATAGGCTGTGTTCCAGCTGAGTTGGCCACGAGAAGTACTAACGGTCAATGCTCAGCCGAGCTGCAAAGAGCTGCAGGTCTCTTTAATCCACAACTTACTCAGATGCTAACTGGACTCAACAGGGAACTGGGTGCCGATATCTTCATTGCTGCTAACACTCAGCAGAGTGCTCTAGACTTCGTGGTTAACCCTCAAGCATATGGATTTACTACAGCGAAAATTGCCTGCTGCGGGCAAGGACCTTACAATGGGCTGGGATTGTGCACTGGATTGTCCAATTTGTGCCCGAACAGAGACGTATATGCTTTCTGGGATTCATTTCATCCATCAGAAAGGGCAAACCGGTACATTGTTCAGAATATTCTGTCCGGCACTACTGAATACATGAACCCCATGAACCTCAGCACCATTTTGGCTTTGGACTCCAGGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

346

Amino Acids

38.07

Weight (kDa)

5.96

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 46 - 321 7.3e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 58, 671
AccB1I GGYRCC 3 cut(s) 59, 257, 737
AccI GTMKAC 1 cut(s) 136
AciI CCGC 4 cut(s) 398, 441, 483, 837
AclWI GGATC 1 cut(s) 394
AcoI YGGCCR 1 cut(s) 630
AcsI RAATTY 1 cut(s) 303
AdeI CACNNNGTG 1 cut(s) 197
AfaI GTAC 4 cut(s) 332, 598, 643, 953
AfiI CCNNNNNNNGG 2 cut(s) 837, 854
AgeI ACCGGT 1 cut(s) 948
AgsI TTSAA 1 cut(s) 353
AjnI CCWGG 1 cut(s) 1031
AluBI AGCT 6 cut(s) 9, 54, 232, 623, 667, 677
AluI AGCT 6 cut(s) 9, 54, 232, 623, 667, 677
Alw21I GWGCWC 4 cut(s) 234, 421, 778, 872
Alw26I GTCTC 2 cut(s) 689, 894
Alw44I GTGCAC 1 cut(s) 868
AlwI GGATC 1 cut(s) 394
AoxI GGCC 2 cut(s) 145, 630
ApaLI GTGCAC 1 cut(s) 868
ApeKI GCWGC 4 cut(s) 667, 677, 755, 834
ApoI RAATTY 1 cut(s) 303
AsiGI ACCGGT 1 cut(s) 948
AspS9I GGNCC 5 cut(s) 34, 145, 602, 845, 1035
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 4 cut(s) 34, 602, 845, 1035
BaeGI GKGCMC 3 cut(s) 379, 872, 893
BalI TGGCCA 1 cut(s) 632
BanI GGYRCC 3 cut(s) 59, 257, 737
BanII GRGCYC 1 cut(s) 234
BauI CACGAG 3 cut(s) 78, 93, 634
Bbv12I GWGCWC 4 cut(s) 234, 421, 778, 872
BbvCI CCTCAGC 1 cut(s) 1007
BbvI GCAGC 4 cut(s) 654, 664, 742, 821
BccI CCATC 1 cut(s) 940
BceAI ACGGC 1 cut(s) 132
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 1 cut(s) 1033
BcoDI GTCTC 2 cut(s) 689, 894
BfaI CTAG 4 cut(s) 6, 486, 779, 1039
BfmI CTRYAG 3 cut(s) 606, 678, 816
BfuAI ACCTGC 2 cut(s) 58, 671
BglII AGATCT 1 cut(s) 488
BisI GCNGC 5 cut(s) 442, 668, 678, 756, 835
BlpI GCTNAGC 1 cut(s) 658
BlsI GCNGC 5 cut(s) 443, 669, 679, 757, 836
BmcAI AGTACT 1 cut(s) 643
Bme1390I CCNGG 1 cut(s) 1033
Bme18I GGWCC 4 cut(s) 34, 602, 845, 1035
BmgT120I GGNCC 5 cut(s) 34, 145, 602, 845, 1035
BmiI GGNNCC 4 cut(s) 61, 259, 474, 739
BmrFI CCNGG 1 cut(s) 1033
BmrI ACTGGG 1 cut(s) 743
BmsI GCATC 1 cut(s) 699
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 743
BpmI CTGGAG 1 cut(s) 1015
Bpu10I CCTNAGC 1 cut(s) 1007
Bpu1102I GCTNAGC 1 cut(s) 658
BpuEI CTTGAG 2 cut(s) 241, 783
BsaI GGTCTC 1 cut(s) 689
BsaWI WCCGGW 2 cut(s) 299, 948
Bsc4I CCNNNNNNNGG 2 cut(s) 837, 854
Bse118I RCCGGY 1 cut(s) 948
Bse1I ACTGG 6 cut(s) 18, 136, 241, 721, 738, 877
Bse3DI GCAATG 1 cut(s) 750
BseBI CCWGG 1 cut(s) 1033
BseGI GGATG 2 cut(s) 168, 928
BseLI CCNNNNNNNGG 2 cut(s) 837, 854
BseMI GCAATG 1 cut(s) 750
BseMII CTCAG 6 cut(s) 525, 615, 672, 719, 779, 1021
BseNI ACTGG 6 cut(s) 18, 136, 241, 721, 738, 877
BseSI GKGCMC 3 cut(s) 379, 872, 893
BseXI GCAGC 4 cut(s) 654, 664, 742, 821
BseYI CCCAGC 2 cut(s) 63, 859
BsgI GTGCAG 1 cut(s) 219
BshFI GGCC 2 cut(s) 147, 632
BshNI GGYRCC 3 cut(s) 59, 257, 737
BshTI ACCGGT 1 cut(s) 948
BsiHKAI GWGCWC 4 cut(s) 234, 421, 778, 872
BsiSI CCGG 3 cut(s) 300, 949, 975
BslI CCNNNNNNNGG 2 cut(s) 837, 854
BsmAI GTCTC 2 cut(s) 689, 894
BsmBI CGTCTC 1 cut(s) 894
BsnI GGCC 2 cut(s) 147, 632
Bso31I GGTCTC 1 cut(s) 689
Bsp1286I GDGCHC 6 cut(s) 234, 379, 421, 778, 872, 893
Bsp1407I TGTACA 1 cut(s) 330
Bsp143I GATC 3 cut(s) 386, 488, 565
Bsp1720I GCTNAGC 1 cut(s) 658
BspACI CCGC 4 cut(s) 398, 441, 483, 837
BspANI GGCC 2 cut(s) 147, 632
BspCNI CTCAG 6 cut(s) 526, 616, 671, 718, 778, 1020
BspHI TCATGA 1 cut(s) 429
BspLI GGNNCC 4 cut(s) 61, 259, 474, 739
BspMAI CTGCAG 1 cut(s) 682
BspMI ACCTGC 2 cut(s) 58, 671
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 394
BspT107I GGYRCC 3 cut(s) 59, 257, 737
BspTNI GGTCTC 1 cut(s) 689
BsrDI GCAATG 1 cut(s) 750
BsrFI RCCGGY 1 cut(s) 948
BsrGI TGTACA 1 cut(s) 330
BsrI ACTGG 6 cut(s) 18, 136, 241, 721, 738, 877
BssAI RCCGGY 1 cut(s) 948
BssMI GATC 3 cut(s) 386, 488, 565
BssSI CACGAG 3 cut(s) 78, 93, 634
Bst2BI CACGAG 3 cut(s) 78, 93, 634
Bst2UI CCWGG 1 cut(s) 1033
Bst4CI ACNGT 2 cut(s) 436, 650
Bst6I CTCTTC 1 cut(s) 510
BstAPI GCANNNNNTGC 1 cut(s) 197
BstAUI TGTACA 1 cut(s) 330
BstC8I GCNNGC 4 cut(s) 7, 277, 832, 839
BstDEI CTNAG 6 cut(s) 534, 624, 658, 705, 765, 1007
BstF5I GGATG 2 cut(s) 168, 928
BstKTI GATC 3 cut(s) 389, 491, 568
BstMAI GTCTC 2 cut(s) 689, 894
BstMBI GATC 3 cut(s) 386, 488, 565
BstMWI GCNNNNNNNGC 5 cut(s) 89, 197, 266, 629, 827
BstNI CCWGG 1 cut(s) 1033
BstSCI CCNGG 1 cut(s) 1031
BstSFI CTRYAG 3 cut(s) 606, 678, 816
BstSLI GKGCMC 3 cut(s) 379, 872, 893
BstV1I GCAGC 4 cut(s) 654, 664, 742, 821
BstX2I RGATCY 2 cut(s) 386, 488
BstYI RGATCY 2 cut(s) 386, 488
BsuRI GGCC 2 cut(s) 147, 632
BtsCI GGATG 2 cut(s) 168, 928
BtsIMutI CAGTG 3 cut(s) 11, 234, 870
BveI ACCTGC 2 cut(s) 58, 671
Cac8I GCNNGC 4 cut(s) 7, 277, 832, 839
CciI TCATGA 1 cut(s) 429
Cfr10I RCCGGY 1 cut(s) 948
Cfr13I GGNCC 5 cut(s) 34, 145, 602, 845, 1035
CseI GACGC 1 cut(s) 110
Csp6I GTAC 4 cut(s) 331, 597, 642, 952
CspAI ACCGGT 1 cut(s) 948
CviAII CATG 3 cut(s) 430, 991, 1000
CviQI GTAC 4 cut(s) 331, 597, 642, 952
DdeI CTNAG 6 cut(s) 534, 624, 658, 705, 765, 1007
DpnI GATC 3 cut(s) 388, 490, 567
DpnII GATC 3 cut(s) 386, 488, 565
DraIII CACNNNGTG 1 cut(s) 197
EaeI YGGCCR 1 cut(s) 630
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
EciI GGCGGA 1 cut(s) 413
Ecl136II GAGCTC 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 689
Eco32I GATATC 1 cut(s) 745
Eco47I GGWCC 4 cut(s) 34, 602, 845, 1035
Eco53kI GAGCTC 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 232
EcoO109I RGGNCCY 3 cut(s) 602, 845, 1035
EcoRI GAATTC 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 1031
EcoRV GATATC 1 cut(s) 745
EcoT38I GRGCYC 1 cut(s) 234
Esp3I CGTCTC 1 cut(s) 894
FaeI CATG 3 cut(s) 433, 994, 1003
FatI CATG 3 cut(s) 429, 990, 999
FauI CCCGC 1 cut(s) 830
FauNDI CATATG 1 cut(s) 805
FblI GTMKAC 1 cut(s) 136
Fnu4HI GCNGC 5 cut(s) 442, 668, 678, 756, 835
FokI GGATG 2 cut(s) 155, 915
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 5 cut(s) 442, 668, 678, 756, 835
FspBI CTAG 4 cut(s) 6, 486, 779, 1039
GluI GCNGC 5 cut(s) 442, 668, 678, 756, 835
GsaI CCCAGC 2 cut(s) 67, 863
GsuI CTGGAG 1 cut(s) 1015
HaeIII GGCC 2 cut(s) 147, 632
HapII CCGG 3 cut(s) 300, 949, 975
HgaI GACGC 1 cut(s) 110
Hin1II CATG 3 cut(s) 433, 994, 1003
HincII GTYRAC 3 cut(s) 137, 457, 793
HindII GTYRAC 3 cut(s) 137, 457, 793
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 6 cut(s) 203, 223, 285, 720, 920, 1028
HpaI GTTAAC 1 cut(s) 793
HpaII CCGG 3 cut(s) 300, 949, 975
Hpy166II GTNNAC 6 cut(s) 137, 316, 412, 457, 793, 870
Hpy188I TCNGA 9 cut(s) 326, 386, 391, 521, 535, 542, 708, 937, 963
Hpy188III TCNNGA 5 cut(s) 227, 353, 430, 509, 779
Hpy8I GTNNAC 6 cut(s) 137, 316, 412, 457, 793, 870
Hpy99I CGWCG 1 cut(s) 104
HpyCH4III ACNGT 2 cut(s) 436, 650
HpyCH4IV ACGT 2 cut(s) 209, 903
HpyCH4V TGCA 5 cut(s) 44, 200, 670, 680, 870
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 197, 266, 629, 827
HpyF3I CTNAG 6 cut(s) 534, 624, 658, 705, 765, 1007
HpySE526I ACGT 2 cut(s) 209, 903
Hsp92II CATG 3 cut(s) 433, 994, 1003
KspAI GTTAAC 1 cut(s) 793
Kzo9I GATC 3 cut(s) 386, 488, 565
LmnI GCTCC 2 cut(s) 400, 416
Lsp1109I GCAGC 4 cut(s) 654, 664, 742, 821
LweI GCATC 1 cut(s) 699
MaeI CTAG 4 cut(s) 6, 486, 779, 1039
MaeII ACGT 2 cut(s) 209, 903
MaeIII GTNAC 1 cut(s) 589
MalI GATC 3 cut(s) 388, 490, 567
MboI GATC 3 cut(s) 386, 488, 565
MboII GAAGA 2 cut(s) 497, 739
MflI RGATCY 2 cut(s) 386, 488
MhlI GDGCHC 6 cut(s) 234, 379, 421, 778, 872, 893
MlsI TGGCCA 1 cut(s) 632
MluCI AATT 5 cut(s) 303, 338, 460, 825, 883
MluNI TGGCCA 1 cut(s) 632
MlyI GAGTC 3 cut(s) 232, 714, 1022
MmeI TCCRAC 1 cut(s) 152
MnlI CCTC 7 cut(s) 67, 299, 536, 549, 566, 807, 1016
Mox20I TGGCCA 1 cut(s) 632
MscI TGGCCA 1 cut(s) 632
MseI TTAA 2 cut(s) 690, 792
Msp20I TGGCCA 1 cut(s) 632
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 3 cut(s) 300, 949, 975
MspR9I CCNGG 1 cut(s) 1033
MvaI CCWGG 1 cut(s) 1033
MwoI GCNNNNNNNGC 5 cut(s) 89, 197, 266, 629, 827
NdeI CATATG 1 cut(s) 805
NdeII GATC 3 cut(s) 386, 488, 565
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 3 cut(s) 433, 994, 1003
NlaIV GGNNCC 4 cut(s) 61, 259, 474, 739
NmeAIII GCCGAG 1 cut(s) 688
PagI TCATGA 1 cut(s) 429
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PfeI GAWTC 3 cut(s) 203, 285, 920
PflFI GACNNNGTC 1 cut(s) 452
PfoI TCCNGGA 1 cut(s) 1031
PinAI ACCGGT 1 cut(s) 948
PkrI GCNGC 5 cut(s) 443, 669, 679, 757, 836
PleI GAGTC 3 cut(s) 231, 714, 1022
PpsI GAGTC 3 cut(s) 231, 714, 1022
PpuMI RGGWCCY 3 cut(s) 602, 845, 1035
Psp124BI GAGCTC 1 cut(s) 234
Psp5II RGGWCCY 3 cut(s) 602, 845, 1035
Psp6I CCWGG 1 cut(s) 1031
PspFI CCCAGC 2 cut(s) 63, 859
PspGI CCWGG 1 cut(s) 1031
PspN4I GGNNCC 4 cut(s) 61, 259, 474, 739
PspPI GGNCC 5 cut(s) 34, 145, 602, 845, 1035
PspPPI RGGWCCY 3 cut(s) 602, 845, 1035
PstI CTGCAG 1 cut(s) 682
PsuI RGATCY 2 cut(s) 386, 488
PsyI GACNNNGTC 1 cut(s) 452
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 4 cut(s) 332, 598, 643, 953
RsaNI GTAC 4 cut(s) 331, 597, 642, 952
SacI GAGCTC 1 cut(s) 234
SalI GTCGAC 1 cut(s) 135
SaqAI TTAA 2 cut(s) 690, 792
SatI GCNGC 5 cut(s) 442, 668, 678, 756, 835
Sau3AI GATC 3 cut(s) 386, 488, 565
Sau96I GGNCC 5 cut(s) 34, 145, 602, 845, 1035
ScaI AGTACT 1 cut(s) 643
SchI GAGTC 3 cut(s) 232, 714, 1022
ScrFI CCNGG 1 cut(s) 1033
SduI GDGCHC 6 cut(s) 234, 379, 421, 778, 872, 893
SfaNI GCATC 1 cut(s) 699
SfcI CTRYAG 3 cut(s) 606, 678, 816
SinI GGWCC 4 cut(s) 34, 602, 845, 1035
SmlI CTYRAG 2 cut(s) 220, 798
SmoI CTYRAG 2 cut(s) 220, 798
Sse9I AATT 5 cut(s) 303, 338, 460, 825, 883
SsiI CCGC 4 cut(s) 398, 441, 483, 837
SspI AATATT 2 cut(s) 500, 967
SspMI CTAG 4 cut(s) 6, 486, 779, 1039
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 1031
TaaI ACNGT 2 cut(s) 436, 650
TaiI ACGT 2 cut(s) 212, 906
TaqI TCGA 2 cut(s) 72, 136
TasI AATT 5 cut(s) 303, 338, 460, 825, 883
TatI WGTACW 2 cut(s) 330, 641
TauI GCSGC 1 cut(s) 444
TfiI GAWTC 3 cut(s) 203, 285, 920
Tru1I TTAA 2 cut(s) 690, 792
Tru9I TTAA 2 cut(s) 690, 792
TscAI CASTG 3 cut(s) 18, 241, 877
TseI GCWGC 4 cut(s) 667, 677, 755, 834
TspDTI ATGAA 6 cut(s) 169, 739, 912, 917, 1007, 1016
TspGWI ACGGA 1 cut(s) 429
TspRI CASTG 3 cut(s) 18, 241, 877
Tth111I GACNNNGTC 1 cut(s) 452
VneI GTGCAC 1 cut(s) 868
VpaK11BI GGWCC 4 cut(s) 34, 602, 845, 1035
XapI RAATTY 1 cut(s) 303
XbaI TCTAGA 1 cut(s) 778
XcmI CCANNNNNNNNNTGG 1 cut(s) 1021
XmiI GTMKAC 1 cut(s) 136
XspI CTAG 4 cut(s) 6, 486, 779, 1039
ZrmI AGTACT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.