MD12G1252500.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007620
Physical Location & Seq
Forward (+)
32163367 .. 32165527
2161 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1252500.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGGCTAGCTCATTGGCCATTTTTGGTGTGCTCATGATCATGGCATTGGGAATGTTGAGTTATGAGGCTGAGGCTCGTGCCTTTTTTGTCTTTGGAGACTCACTGGTCGACAATGGCAACAACAACTACTTAGCCACCACCGCTCGTGCTGACTCTCCTCCTTACGGCATTGACTATCCCACCGGCCGACCCACCGGCCGTTTCTCCAATGGCATGAACATTCCTGACTTCATCAGCCAGCAACTTGGCGCAGAACCTACATTGCCATACTTGAGTCCAGAGCTCACAGGGCAAAAACTACTAGTTGGTGCAAACTTTGCCTCTGCTGGCATTGGAATTCTCAATGACACTGGGATTCAGTTTATAAATATAATCAGAATGACAAGGCAATTCGACTACTTCCAACAATACCAGCAAAGGCTGTCTTCCCTAATCGGACCTCAACGTACCAAACAGCTCGTAAATCAAGCACTCGTCCTCCTCACCGTCGGCGGCAACGATTTTGTCAACAACTACTACCTGGTGCCTTTCTCCGCAAGATCTCGCCAGTTTGCTCTGCCAGACTATGTCAAATACCTCATTTCTGAGCTCCGAAAACTTCTCTTGAAAATGTATGATCTTGGAGTACGAAGGGTTTTGGTGACGGGGACAGGACCACTAGGCTGTGTGCCGGCCGAATTGGCCATGAGAAGCAGGAACGGAGAATGCTCGGCGGAGCTGCAACGAGCGGCGTCTCTGTACAACCCCCAACTCGTTCAGTTGCTGAGATCACTCAACAGCCAACTCGGTTCCGATGTCTTCATTGCTGCAAACACTCAGCAGACGCGCAATGACTTCGTCAGCAACCCTCAAGCTTATGGATTTTCTACGTCAAAGATTGCATGCTGTGGACAAGGACCTTACAATGGCTTGGGGCTGTGCACGGTGGCCTCAAATTTGTGCCCGAACCGCAACCAATACGCTTTCTGGGATGCATTTCATCCAACTGAAAAGGCGAACAAACTGATCGTTCAGAATATTTTGACAGGGGATGAAAAGTACATGTACCCCATGAACCTCAGCACCATCTTGGCTTTGGATTCCAGGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

364

Amino Acids

40.0

Weight (kDa)

8.65

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 339 1.7e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 365
AasI GACNNNNNNGTC 1 cut(s) 104
AccB1I GGYRCC 1 cut(s) 523
AccBSI CCGCTC 2 cut(s) 143, 728
AccI GTMKAC 1 cut(s) 108
AccII CGCG 1 cut(s) 826
AciI CCGC 6 cut(s) 141, 492, 534, 713, 728, 949
AcoI YGGCCR 5 cut(s) 15, 184, 196, 672, 681
AcsI RAATTY 2 cut(s) 336, 934
AcyI GRCGYC 1 cut(s) 731
AfaI GTAC 5 cut(s) 448, 627, 740, 1040, 1046
AfiI CCNNNNNNNGG 2 cut(s) 164, 905
AflIII ACRYGT 1 cut(s) 1041
AgsI TTSAA 1 cut(s) 607
AhlI ACTAGT 1 cut(s) 301
AjnI CCWGG 2 cut(s) 519, 1082
AluBI AGCT 6 cut(s) 9, 283, 457, 589, 718, 854
AluI AGCT 6 cut(s) 9, 283, 457, 589, 718, 854
Alw21I GWGCWC 4 cut(s) 33, 285, 591, 923
Alw26I GTCTC 2 cut(s) 90, 738
Alw44I GTGCAC 1 cut(s) 919
AlwNI CAGNNNCTG 1 cut(s) 763
AoxI GGCC 6 cut(s) 15, 184, 196, 672, 681, 927
ApaLI GTGCAC 1 cut(s) 919
ApeKI GCWGC 2 cut(s) 718, 806
ApoI RAATTY 2 cut(s) 336, 934
AspLEI GCGC 2 cut(s) 251, 828
AspS9I GGNCC 4 cut(s) 437, 653, 896, 1086
AsuHPI GGTGA 2 cut(s) 475, 652
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 4 cut(s) 437, 653, 896, 1086
BaeGI GKGCMC 2 cut(s) 923, 944
BalI TGGCCA 2 cut(s) 17, 683
BanI GGYRCC 1 cut(s) 523
BanII GRGCYC 2 cut(s) 285, 591
BauI CACGAG 2 cut(s) 75, 144
BbsI GAAGAC 2 cut(s) 417, 790
Bbv12I GWGCWC 4 cut(s) 33, 285, 591, 923
BbvCI CCTCAGC 2 cut(s) 69, 1058
BbvI GCAGC 2 cut(s) 705, 793
BccI CCATC 1 cut(s) 1073
BceAI ACGGC 2 cut(s) 181, 183
BcgI CGANNNNNNTGC 2 cut(s) 817, 851
BciT130I CCWGG 2 cut(s) 521, 1084
BclI TGATCA 1 cut(s) 36
BcoDI GTCTC 2 cut(s) 90, 738
BcuI ACTAGT 1 cut(s) 301
BfaI CTAG 3 cut(s) 6, 302, 659
BglI GCCNNNNNGGC 1 cut(s) 680
BglII AGATCT 1 cut(s) 539
BisI GCNGC 4 cut(s) 493, 719, 729, 807
BlsI GCNGC 4 cut(s) 494, 720, 730, 808
Bme1390I CCNGG 2 cut(s) 521, 1084
Bme18I GGWCC 4 cut(s) 437, 653, 896, 1086
BmgT120I GGNCC 4 cut(s) 437, 653, 896, 1086
BmiI GGNNCC 2 cut(s) 525, 790
BmrFI CCNGG 2 cut(s) 521, 1084
BmrI ACTGGG 1 cut(s) 360
BmsI GCATC 1 cut(s) 961
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 360
BpiI GAAGAC 2 cut(s) 417, 790
Bpu10I CCTNAGC 2 cut(s) 69, 1058
BpuEI CTTGAG 2 cut(s) 292, 834
BsaHI GRCGYC 1 cut(s) 731
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 905
Bse118I RCCGGY 3 cut(s) 182, 194, 670
Bse1I ACTGG 3 cut(s) 108, 355, 547
Bse3DI GCAATG 3 cut(s) 260, 801, 835
BseBI CCWGG 2 cut(s) 521, 1084
BseGI GGATG 3 cut(s) 976, 979, 1036
BseLI CCNNNNNNNGG 2 cut(s) 164, 905
BseMI GCAATG 3 cut(s) 260, 801, 835
BseMII CTCAG 5 cut(s) 60, 576, 755, 830, 1072
BseNI ACTGG 3 cut(s) 108, 355, 547
BseRI GAGGAG 2 cut(s) 147, 470
BseSI GKGCMC 2 cut(s) 923, 944
BseX3I CGGCCG 3 cut(s) 184, 196, 672
BseXI GCAGC 2 cut(s) 705, 793
Bsh1236I CGCG 1 cut(s) 826
Bsh1285I CGRYCG 3 cut(s) 187, 199, 675
BshFI GGCC 6 cut(s) 17, 186, 198, 674, 683, 929
BshNI GGYRCC 1 cut(s) 523
BsiEI CGRYCG 3 cut(s) 187, 199, 675
BsiHKAI GWGCWC 4 cut(s) 33, 285, 591, 923
BsiSI CCGG 3 cut(s) 183, 195, 671
BslFI GGGAC 1 cut(s) 661
BslI CCNNNNNNNGG 2 cut(s) 164, 905
BsmAI GTCTC 2 cut(s) 90, 738
BsmBI CGTCTC 1 cut(s) 738
BsmFI GGGAC 1 cut(s) 661
BsmI GAATGC 1 cut(s) 710
BsnI GGCC 6 cut(s) 17, 186, 198, 674, 683, 929
Bsp1286I GDGCHC 5 cut(s) 33, 285, 591, 923, 944
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 5 cut(s) 36, 539, 616, 767, 1005
BspACI CCGC 6 cut(s) 141, 492, 534, 713, 728, 949
BspANI GGCC 6 cut(s) 17, 186, 198, 674, 683, 929
BspCNI CTCAG 5 cut(s) 61, 577, 756, 829, 1071
BspFNI CGCG 1 cut(s) 826
BspHI TCATGA 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 525, 790
BspOI GCTAGC 1 cut(s) 9
BspT107I GGYRCC 1 cut(s) 523
BsrBI CCGCTC 2 cut(s) 143, 728
BsrDI GCAATG 3 cut(s) 260, 801, 835
BsrFI RCCGGY 3 cut(s) 182, 194, 670
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 3 cut(s) 108, 355, 547
BssAI RCCGGY 3 cut(s) 182, 194, 670
BssMI GATC 5 cut(s) 36, 539, 616, 767, 1005
BssNI GRCGYC 1 cut(s) 731
BssSI CACGAG 2 cut(s) 75, 144
Bst2BI CACGAG 2 cut(s) 75, 144
Bst2UI CCWGG 2 cut(s) 521, 1084
Bst4CI ACNGT 2 cut(s) 487, 925
BstACI GRCGYC 1 cut(s) 731
BstAPI GCANNNNNTGC 1 cut(s) 317
BstAUI TGTACA 1 cut(s) 738
BstC8I GCNNGC 5 cut(s) 7, 239, 328, 672, 883
BstDEI CTNAG 6 cut(s) 69, 130, 585, 764, 816, 1058
BstF5I GGATG 3 cut(s) 976, 979, 1036
BstFNI CGCG 1 cut(s) 826
BstHHI GCGC 2 cut(s) 251, 828
BstKTI GATC 5 cut(s) 39, 542, 619, 770, 1008
BstMAI GTCTC 2 cut(s) 90, 738
BstMBI GATC 5 cut(s) 36, 539, 616, 767, 1005
BstMCI CGRYCG 3 cut(s) 187, 199, 675
BstMWI GCNNNNNNNGC 5 cut(s) 140, 289, 317, 680, 948
BstNI CCWGG 2 cut(s) 521, 1084
BstNSI RCATGY 2 cut(s) 885, 1045
BstSCI CCNGG 2 cut(s) 519, 1082
BstSLI GKGCMC 2 cut(s) 923, 944
BstUI CGCG 1 cut(s) 826
BstV1I GCAGC 2 cut(s) 705, 793
BstV2I GAAGAC 2 cut(s) 417, 790
BstX2I RGATCY 1 cut(s) 539
BstXI CCANNNNNNTGG 1 cut(s) 245
BstYI RGATCY 1 cut(s) 539
BstZI CGGCCG 3 cut(s) 184, 196, 672
BsuRI GGCC 6 cut(s) 17, 186, 198, 674, 683, 929
BtsCI GGATG 3 cut(s) 976, 979, 1036
BtsIMutI CAGTG 2 cut(s) 101, 348
Cac8I GCNNGC 5 cut(s) 7, 239, 328, 672, 883
CaiI CAGNNNCTG 1 cut(s) 763
CciI TCATGA 1 cut(s) 33
CfoI GCGC 2 cut(s) 251, 828
Cfr10I RCCGGY 3 cut(s) 182, 194, 670
Cfr13I GGNCC 4 cut(s) 437, 653, 896, 1086
CseI GACGC 2 cut(s) 720, 832
CsiI ACCWGGT 1 cut(s) 519
Csp6I GTAC 5 cut(s) 447, 626, 739, 1039, 1045
CviAII CATG 7 cut(s) 34, 40, 214, 685, 882, 1042, 1051
CviQI GTAC 5 cut(s) 447, 626, 739, 1039, 1045
DdeI CTNAG 6 cut(s) 69, 130, 585, 764, 816, 1058
DpnI GATC 5 cut(s) 38, 541, 618, 769, 1007
DpnII GATC 5 cut(s) 36, 539, 616, 767, 1005
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 5 cut(s) 15, 184, 196, 672, 681
EagI CGGCCG 3 cut(s) 184, 196, 672
EciI GGCGGA 1 cut(s) 728
Ecl136II GAGCTC 2 cut(s) 283, 589
EclXI CGGCCG 3 cut(s) 184, 196, 672
Eco24I GRGCYC 2 cut(s) 285, 591
Eco47I GGWCC 4 cut(s) 437, 653, 896, 1086
Eco52I CGGCCG 3 cut(s) 184, 196, 672
Eco53kI GAGCTC 2 cut(s) 283, 589
EcoICRI GAGCTC 2 cut(s) 283, 589
EcoO109I RGGNCCY 2 cut(s) 896, 1086
EcoRI GAATTC 1 cut(s) 336
EcoRII CCWGG 2 cut(s) 519, 1082
EcoT22I ATGCAT 1 cut(s) 976
EcoT38I GRGCYC 2 cut(s) 285, 591
Esp3I CGTCTC 1 cut(s) 738
FaeI CATG 7 cut(s) 37, 43, 217, 688, 885, 1045, 1054
FalI AAGNNNNNCTT 2 cut(s) 409, 441
FaqI GGGAC 1 cut(s) 661
FatI CATG 7 cut(s) 33, 39, 213, 684, 881, 1041, 1050
FbaI TGATCA 1 cut(s) 36
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 4 cut(s) 493, 719, 729, 807
FokI GGATG 3 cut(s) 966, 983, 1043
FriOI GRGCYC 2 cut(s) 285, 591
Fsp4HI GCNGC 4 cut(s) 493, 719, 729, 807
FspBI CTAG 3 cut(s) 6, 302, 659
GlaI GCGC 2 cut(s) 250, 827
GluI GCNGC 4 cut(s) 493, 719, 729, 807
HaeIII GGCC 6 cut(s) 17, 186, 198, 674, 683, 929
HapII CCGG 3 cut(s) 183, 195, 671
HgaI GACGC 2 cut(s) 720, 832
HhaI GCGC 2 cut(s) 251, 828
Hin1I GRCGYC 1 cut(s) 731
Hin1II CATG 7 cut(s) 37, 43, 217, 688, 885, 1045, 1054
Hin6I GCGC 2 cut(s) 249, 826
HinP1I GCGC 2 cut(s) 249, 826
HincII GTYRAC 2 cut(s) 109, 508
HindII GTYRAC 2 cut(s) 109, 508
HindIII AAGCTT 1 cut(s) 852
HinfI GANTC 5 cut(s) 98, 152, 274, 355, 1079
HpaII CCGG 3 cut(s) 183, 195, 671
HphI GGTGA 2 cut(s) 475, 652
Hpy166II GTNNAC 4 cut(s) 109, 508, 890, 921
Hpy188I TCNGA 6 cut(s) 377, 437, 586, 593, 793, 1014
Hpy188III TCNNGA 4 cut(s) 34, 224, 278, 604
Hpy8I GTNNAC 4 cut(s) 109, 508, 890, 921
Hpy99I CGWCG 1 cut(s) 491
HpyAV CCTTC 1 cut(s) 624
HpyCH4III ACNGT 2 cut(s) 487, 925
HpyCH4IV ACGT 2 cut(s) 445, 869
HpyCH4V TGCA 6 cut(s) 311, 721, 809, 881, 921, 974
HpyF10VI GCNNNNNNNGC 5 cut(s) 140, 289, 317, 680, 948
HpyF3I CTNAG 6 cut(s) 69, 130, 585, 764, 816, 1058
HpySE526I ACGT 2 cut(s) 445, 869
Hsp92I GRCGYC 1 cut(s) 731
Hsp92II CATG 7 cut(s) 37, 43, 217, 688, 885, 1045, 1054
HspAI GCGC 2 cut(s) 249, 826
KroI GCCGGC 1 cut(s) 670
KroNI GCCGGC 1 cut(s) 672
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 5 cut(s) 36, 539, 616, 767, 1005
LmnI GCTCC 2 cut(s) 594, 715
Lsp1109I GCAGC 2 cut(s) 705, 793
LweI GCATC 1 cut(s) 961
MabI ACCWGGT 1 cut(s) 519
MaeI CTAG 3 cut(s) 6, 302, 659
MaeII ACGT 2 cut(s) 445, 869
MaeIII GTNAC 1 cut(s) 640
MalI GATC 5 cut(s) 38, 541, 618, 769, 1007
MbiI CCGCTC 2 cut(s) 143, 728
MboI GATC 5 cut(s) 36, 539, 616, 767, 1005
MboII GAAGA 2 cut(s) 417, 790
MflI RGATCY 1 cut(s) 539
MhlI GDGCHC 5 cut(s) 33, 285, 591, 923, 944
MlsI TGGCCA 2 cut(s) 17, 683
MluCI AATT 4 cut(s) 336, 389, 677, 934
MluNI TGGCCA 2 cut(s) 17, 683
MlyI GAGTC 3 cut(s) 92, 146, 283
MmeI TCCRAC 2 cut(s) 427, 1007
Mox20I TGGCCA 2 cut(s) 17, 683
Mph1103I ATGCAT 1 cut(s) 976
MroNI GCCGGC 1 cut(s) 670
MscI TGGCCA 2 cut(s) 17, 683
MslI CAYNNNNRTG 1 cut(s) 38
Msp20I TGGCCA 2 cut(s) 17, 683
MspI CCGG 3 cut(s) 183, 195, 671
MspR9I CCNGG 2 cut(s) 521, 1084
Mva1269I GAATGC 1 cut(s) 710
MvaI CCWGG 2 cut(s) 521, 1084
MvnI CGCG 1 cut(s) 826
MwoI GCNNNNNNNGC 5 cut(s) 140, 289, 317, 680, 948
NaeI GCCGGC 1 cut(s) 672
NdeII GATC 5 cut(s) 36, 539, 616, 767, 1005
NgoMIV GCCGGC 1 cut(s) 670
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 7 cut(s) 37, 43, 217, 688, 885, 1045, 1054
NlaIV GGNNCC 2 cut(s) 525, 790
NmeAIII GCCGAG 1 cut(s) 689
NmuCI GTSAC 1 cut(s) 640
NsiI ATGCAT 1 cut(s) 976
NspI RCATGY 2 cut(s) 885, 1045
PaeI GCATGC 1 cut(s) 885
PagI TCATGA 1 cut(s) 33
PciI ACATGT 1 cut(s) 1041
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PctI GAATGC 1 cut(s) 710
PdiI GCCGGC 1 cut(s) 672
PfeI GAWTC 2 cut(s) 355, 1079
PflFI GACNNNGTC 2 cut(s) 566, 836
PfoI TCCNGGA 1 cut(s) 1082
PkrI GCNGC 4 cut(s) 494, 720, 730, 808
PleI GAGTC 3 cut(s) 92, 146, 282
PpsI GAGTC 3 cut(s) 92, 146, 282
PpuMI RGGWCCY 2 cut(s) 896, 1086
PscI ACATGT 1 cut(s) 1041
PsiI TTATAA 1 cut(s) 365
Psp124BI GAGCTC 2 cut(s) 285, 591
Psp5II RGGWCCY 2 cut(s) 896, 1086
Psp6I CCWGG 2 cut(s) 519, 1082
PspGI CCWGG 2 cut(s) 519, 1082
PspN4I GGNNCC 2 cut(s) 525, 790
PspPI GGNCC 4 cut(s) 437, 653, 896, 1086
PspPPI RGGWCCY 2 cut(s) 896, 1086
PstNI CAGNNNCTG 1 cut(s) 763
PsuI RGATCY 1 cut(s) 539
PsyI GACNNNGTC 2 cut(s) 566, 836
RsaI GTAC 5 cut(s) 448, 627, 740, 1040, 1046
RsaNI GTAC 5 cut(s) 447, 626, 739, 1039, 1045
RseI CAYNNNNRTG 1 cut(s) 38
SacI GAGCTC 2 cut(s) 285, 591
SalI GTCGAC 1 cut(s) 107
SatI GCNGC 4 cut(s) 493, 719, 729, 807
Sau3AI GATC 5 cut(s) 36, 539, 616, 767, 1005
Sau96I GGNCC 4 cut(s) 437, 653, 896, 1086
SchI GAGTC 3 cut(s) 92, 146, 283
ScrFI CCNGG 2 cut(s) 521, 1084
SduI GDGCHC 5 cut(s) 33, 285, 591, 923, 944
SexAI ACCWGGT 1 cut(s) 519
SfaNI GCATC 1 cut(s) 961
SfiI GGCCNNNNNGGCC 1 cut(s) 680
SinI GGWCC 4 cut(s) 437, 653, 896, 1086
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 271, 849
SmoI CTYRAG 2 cut(s) 271, 849
SpeI ACTAGT 1 cut(s) 301
SphI GCATGC 1 cut(s) 885
Sse9I AATT 4 cut(s) 336, 389, 677, 934
SsiI CCGC 6 cut(s) 141, 492, 534, 713, 728, 949
SspI AATATT 1 cut(s) 1018
SspMI CTAG 3 cut(s) 6, 302, 659
SstI GAGCTC 2 cut(s) 285, 591
StyD4I CCNGG 2 cut(s) 519, 1082
TaaI ACNGT 2 cut(s) 487, 925
TaiI ACGT 2 cut(s) 448, 872
TaqI TCGA 2 cut(s) 108, 393
TasI AATT 4 cut(s) 336, 389, 677, 934
TatI WGTACW 2 cut(s) 738, 1038
TauI GCSGC 2 cut(s) 495, 731
TfiI GAWTC 2 cut(s) 355, 1079
TscAI CASTG 2 cut(s) 108, 355
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 2 cut(s) 718, 806
Tsp45I GTSAC 1 cut(s) 640
TspDTI ATGAA 6 cut(s) 220, 230, 790, 968, 1047, 1067
TspGWI ACGGA 1 cut(s) 714
TspRI CASTG 2 cut(s) 108, 355
Tth111I GACNNNGTC 2 cut(s) 566, 836
VneI GTGCAC 1 cut(s) 919
VpaK11BI GGWCC 4 cut(s) 437, 653, 896, 1086
XapI RAATTY 2 cut(s) 336, 934
XceI RCATGY 2 cut(s) 885, 1045
XcmI CCANNNNNNNNNTGG 1 cut(s) 1072
XmiI GTMKAC 1 cut(s) 108
XspI CTAG 3 cut(s) 6, 302, 659
Zsp2I ATGCAT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.