Rh3DG016200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0007620
Physical Location & Seq
Reverse (-)
986825 .. 989339
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG016200.1

Sequence Viewer

Length: 870 bp
ATGGCTAGCTCACTGGTGCTGATTTTCAGTCTAGTCCTCACTGCATTGGAAAGCTTCGGCACCCAGCAGGTCGAGGCTCGTGCCTTTTTCGTGTTCGGAGACTCCTTGGTCGACAATGGCAACAACAATTACTTACTAACCCAGGCTCGTGCCGACGCTTATCCTTACGGCATTGACTATCCAACTGGTCGACCAACGGGCCGTTTCTCCAATGGCTACAACATCCCTGACTTCATCAGTCAAGCACTTGGTGCAGAATCCACGTTGCCTTACTTGAGTCCAGAGCTCAATGGACAAAGGCTACTTGTTGGTGCCAACTTTGCTTCTGCTGGCATTGGAATCCTCAATGACACCGGAATTCAGTTTGTAAACATAATCAGAATGTACAGACAATTTGAGTATTTTCAAGAATACCAGCAAAGAGTGAGTGCCCTTATCGGATCTGAACAGACGGAGCGACTTGTAAACGGAGCACTTATTCTCATGACTGTTGGCGGCAACGACTTTGTCAACAATTACTACTTGGTTCCCTACTCTGCTAGATCTCGTCAATATTCCCTTCCAGATTACGTCAAATACCTCATTTCTGAGTTCAGAAACCTTCTTTTGAGGCTATACGATCTTGGAGGGCGTAGGGTTTTGGTGACAGGTACAGGACCATTAGGCTGTGTTCCAGCTGAGTTGGCCACGAGAAGTTCTAACGGTCAATGCTCAGCCGAGCTGCAAAGAGCTGCAGGTCTCTTTAATCCACAACTTACTCAGATGCTAAGTGGACTCAACAGGGAACTGGGTGCCGATATCTTCATTGCTGCTAACACTCAGCAGAGTGCTAGTGACTTCGTGGTTAACCCTCAAGCATATGGTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

289

Amino Acids

31.71

Weight (kDa)

4.97

Isoelectric Point (pI)

29.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 262 2e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
Acc36I ACCTGC 2 cut(s) 58, 725
AccB1I GGYRCC 3 cut(s) 59, 311, 791
AccI GTMKAC 2 cut(s) 111, 190
AciI CCGC 1 cut(s) 495
AclWI GGATC 1 cut(s) 448
AcoI YGGCCR 1 cut(s) 684
AcsI RAATTY 1 cut(s) 357
AdeI CACNNNGTG 1 cut(s) 251
AfaI GTAC 3 cut(s) 386, 652, 865
AgsI TTSAA 1 cut(s) 407
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 6 cut(s) 9, 54, 286, 677, 721, 731
AluI AGCT 6 cut(s) 9, 54, 286, 677, 721, 731
Alw21I GWGCWC 2 cut(s) 288, 475
Alw26I GTCTC 2 cut(s) 93, 743
AlwI GGATC 1 cut(s) 448
AoxI GGCC 2 cut(s) 199, 684
ApeKI GCWGC 3 cut(s) 721, 731, 809
ApoI RAATTY 1 cut(s) 357
AspS9I GGNCC 2 cut(s) 199, 656
AsuHPI GGTGA 1 cut(s) 655
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 1 cut(s) 656
BaeGI GKGCMC 1 cut(s) 433
BalI TGGCCA 1 cut(s) 686
BanI GGYRCC 3 cut(s) 59, 311, 791
BanII GRGCYC 1 cut(s) 288
BauI CACGAG 3 cut(s) 78, 147, 688
Bbv12I GWGCWC 2 cut(s) 288, 475
BbvI GCAGC 3 cut(s) 708, 718, 796
BceAI ACGGC 2 cut(s) 184, 186
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 1 cut(s) 143
BcoDI GTCTC 2 cut(s) 93, 743
BfaI CTAG 4 cut(s) 6, 32, 540, 831
BfmI CTRYAG 1 cut(s) 732
BfuAI ACCTGC 2 cut(s) 58, 725
BglII AGATCT 1 cut(s) 542
BisI GCNGC 4 cut(s) 496, 722, 732, 810
BlpI GCTNAGC 1 cut(s) 712
BlsI GCNGC 4 cut(s) 497, 723, 733, 811
Bme1390I CCNGG 1 cut(s) 143
Bme18I GGWCC 1 cut(s) 656
BmgT120I GGNCC 2 cut(s) 199, 656
BmiI GGNNCC 4 cut(s) 61, 313, 528, 793
BmrFI CCNGG 1 cut(s) 143
BmrI ACTGGG 1 cut(s) 797
BmsI GCATC 1 cut(s) 753
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 797
Bpu1102I GCTNAGC 1 cut(s) 712
BpuEI CTTGAG 2 cut(s) 295, 837
BsaAI YACGTR 1 cut(s) 867
BsaI GGTCTC 1 cut(s) 743
BsaJI CCNNGG 2 cut(s) 105, 141
BsaWI WCCGGW 1 cut(s) 353
Bse1I ACTGG 3 cut(s) 18, 190, 792
Bse3DI GCAATG 1 cut(s) 804
BseBI CCWGG 1 cut(s) 143
BseDI CCNNGG 2 cut(s) 105, 141
BseGI GGATG 1 cut(s) 222
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 5 cut(s) 579, 669, 726, 773, 833
BseNI ACTGG 3 cut(s) 18, 190, 792
BseSI GKGCMC 1 cut(s) 433
BseXI GCAGC 3 cut(s) 708, 718, 796
BseYI CCCAGC 1 cut(s) 63
BsgI GTGCAG 1 cut(s) 273
BshFI GGCC 2 cut(s) 201, 686
BshNI GGYRCC 3 cut(s) 59, 311, 791
BsiHKAI GWGCWC 2 cut(s) 288, 475
BsiSI CCGG 1 cut(s) 354
BsmAI GTCTC 2 cut(s) 93, 743
BsnI GGCC 2 cut(s) 201, 686
Bso31I GGTCTC 1 cut(s) 743
Bsp1286I GDGCHC 3 cut(s) 288, 433, 475
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 3 cut(s) 440, 542, 619
Bsp1720I GCTNAGC 1 cut(s) 712
BspACI CCGC 1 cut(s) 495
BspANI GGCC 2 cut(s) 201, 686
BspCNI CTCAG 5 cut(s) 580, 670, 725, 772, 832
BspHI TCATGA 1 cut(s) 483
BspLI GGNNCC 4 cut(s) 61, 313, 528, 793
BspMAI CTGCAG 1 cut(s) 736
BspMI ACCTGC 2 cut(s) 58, 725
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 448
BspT107I GGYRCC 3 cut(s) 59, 311, 791
BspTNI GGTCTC 1 cut(s) 743
BsrDI GCAATG 1 cut(s) 804
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 3 cut(s) 18, 190, 792
BssECI CCNNGG 2 cut(s) 105, 141
BssMI GATC 3 cut(s) 440, 542, 619
BssSI CACGAG 3 cut(s) 78, 147, 688
BssT1I CCWWGG 1 cut(s) 105
Bst2BI CACGAG 3 cut(s) 78, 147, 688
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 490, 704
BstAPI GCANNNNNTGC 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 384
BstBAI YACGTR 1 cut(s) 867
BstC8I GCNNGC 2 cut(s) 7, 331
BstDEI CTNAG 6 cut(s) 588, 678, 712, 759, 767, 819
BstF5I GGATG 1 cut(s) 222
BstKTI GATC 3 cut(s) 443, 545, 622
BstMAI GTCTC 2 cut(s) 93, 743
BstMBI GATC 3 cut(s) 440, 542, 619
BstMWI GCNNNNNNNGC 3 cut(s) 251, 320, 683
BstNI CCWGG 1 cut(s) 143
BstSCI CCNGG 1 cut(s) 141
BstSFI CTRYAG 1 cut(s) 732
BstSLI GKGCMC 1 cut(s) 433
BstSNI TACGTA 1 cut(s) 867
BstV1I GCAGC 3 cut(s) 708, 718, 796
BstX2I RGATCY 2 cut(s) 440, 542
BstYI RGATCY 2 cut(s) 440, 542
BsuRI GGCC 2 cut(s) 201, 686
BtsCI GGATG 1 cut(s) 222
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 2 cut(s) 11, 39
BveI ACCTGC 2 cut(s) 58, 725
Cac8I GCNNGC 2 cut(s) 7, 331
CciI TCATGA 1 cut(s) 483
Cfr13I GGNCC 2 cut(s) 199, 656
CseI GACGC 1 cut(s) 164
Csp6I GTAC 3 cut(s) 385, 651, 864
CviAII CATG 1 cut(s) 484
CviQI GTAC 3 cut(s) 385, 651, 864
DdeI CTNAG 6 cut(s) 588, 678, 712, 759, 767, 819
DpnI GATC 3 cut(s) 442, 544, 621
DpnII GATC 3 cut(s) 440, 542, 619
DraIII CACNNNGTG 1 cut(s) 251
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 1 cut(s) 684
Ecl136II GAGCTC 1 cut(s) 286
Eco105I TACGTA 1 cut(s) 867
Eco130I CCWWGG 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 743
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 1 cut(s) 656
Eco53kI GAGCTC 1 cut(s) 286
EcoICRI GAGCTC 1 cut(s) 286
EcoRI GAATTC 1 cut(s) 357
EcoRII CCWGG 1 cut(s) 141
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 1 cut(s) 105
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 1 cut(s) 487
FaiI YATR 5 cut(s) 374, 485, 616, 859, 861
FatI CATG 1 cut(s) 483
FauNDI CATATG 1 cut(s) 859
FblI GTMKAC 2 cut(s) 111, 190
Fnu4HI GCNGC 4 cut(s) 496, 722, 732, 810
FokI GGATG 1 cut(s) 209
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 4 cut(s) 496, 722, 732, 810
FspBI CTAG 4 cut(s) 6, 32, 540, 831
GluI GCNGC 4 cut(s) 496, 722, 732, 810
GsaI CCCAGC 1 cut(s) 67
HaeIII GGCC 2 cut(s) 201, 686
HapII CCGG 1 cut(s) 354
HgaI GACGC 1 cut(s) 164
Hin1II CATG 1 cut(s) 487
HincII GTYRAC 4 cut(s) 112, 191, 511, 847
HindII GTYRAC 4 cut(s) 112, 191, 511, 847
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 5 cut(s) 101, 257, 277, 339, 774
HpaI GTTAAC 1 cut(s) 847
HpaII CCGG 1 cut(s) 354
HphI GGTGA 1 cut(s) 655
Hpy166II GTNNAC 7 cut(s) 112, 191, 370, 466, 511, 773, 847
Hpy188I TCNGA 7 cut(s) 98, 380, 440, 445, 589, 596, 762
Hpy188III TCNNGA 4 cut(s) 281, 407, 484, 563
Hpy8I GTNNAC 7 cut(s) 112, 191, 370, 466, 511, 773, 847
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 2 cut(s) 569, 611
HpyCH4III ACNGT 2 cut(s) 490, 704
HpyCH4IV ACGT 3 cut(s) 263, 570, 866
HpyCH4V TGCA 4 cut(s) 44, 254, 724, 734
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 320, 683
HpyF3I CTNAG 6 cut(s) 588, 678, 712, 759, 767, 819
HpySE526I ACGT 3 cut(s) 263, 570, 866
Hsp92II CATG 1 cut(s) 487
KspAI GTTAAC 1 cut(s) 847
Kzo9I GATC 3 cut(s) 440, 542, 619
LmnI GCTCC 2 cut(s) 454, 470
Lsp1109I GCAGC 3 cut(s) 708, 718, 796
LweI GCATC 1 cut(s) 753
MaeI CTAG 4 cut(s) 6, 32, 540, 831
MaeII ACGT 3 cut(s) 263, 570, 866
MaeIII GTNAC 2 cut(s) 643, 833
MalI GATC 3 cut(s) 442, 544, 621
MboI GATC 3 cut(s) 440, 542, 619
MboII GAAGA 1 cut(s) 793
MflI RGATCY 2 cut(s) 440, 542
MhlI GDGCHC 3 cut(s) 288, 433, 475
MlsI TGGCCA 1 cut(s) 686
MluCI AATT 4 cut(s) 127, 357, 392, 514
MluNI TGGCCA 1 cut(s) 686
MlyI GAGTC 3 cut(s) 95, 286, 768
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 7 cut(s) 47, 67, 353, 590, 603, 620, 861
Mox20I TGGCCA 1 cut(s) 686
MscI TGGCCA 1 cut(s) 686
MseI TTAA 2 cut(s) 744, 846
Msp20I TGGCCA 1 cut(s) 686
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 1 cut(s) 143
MvaI CCWGG 1 cut(s) 143
MwoI GCNNNNNNNGC 3 cut(s) 251, 320, 683
NdeI CATATG 1 cut(s) 859
NdeII GATC 3 cut(s) 440, 542, 619
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 1 cut(s) 487
NlaIV GGNNCC 4 cut(s) 61, 313, 528, 793
NmeAIII GCCGAG 1 cut(s) 742
NmuCI GTSAC 2 cut(s) 643, 833
PagI TCATGA 1 cut(s) 483
PfeI GAWTC 2 cut(s) 257, 339
PflFI GACNNNGTC 1 cut(s) 506
PkrI GCNGC 4 cut(s) 497, 723, 733, 811
PleI GAGTC 3 cut(s) 95, 285, 768
PpsI GAGTC 3 cut(s) 95, 285, 768
Ppu21I YACGTR 1 cut(s) 867
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 141
PspFI CCCAGC 1 cut(s) 63
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 4 cut(s) 61, 313, 528, 793
PspPI GGNCC 2 cut(s) 199, 656
PstI CTGCAG 1 cut(s) 736
PsuI RGATCY 2 cut(s) 440, 542
PsyI GACNNNGTC 1 cut(s) 506
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 3 cut(s) 386, 652, 865
RsaNI GTAC 3 cut(s) 385, 651, 864
SacI GAGCTC 1 cut(s) 288
SalI GTCGAC 2 cut(s) 110, 189
SaqAI TTAA 2 cut(s) 744, 846
SatI GCNGC 4 cut(s) 496, 722, 732, 810
Sau3AI GATC 3 cut(s) 440, 542, 619
Sau96I GGNCC 2 cut(s) 199, 656
SchI GAGTC 3 cut(s) 95, 286, 768
ScrFI CCNGG 1 cut(s) 143
SduI GDGCHC 3 cut(s) 288, 433, 475
SfaNI GCATC 1 cut(s) 753
SfcI CTRYAG 1 cut(s) 732
SinI GGWCC 1 cut(s) 656
SmlI CTYRAG 2 cut(s) 274, 852
SmoI CTYRAG 2 cut(s) 274, 852
SnaBI TACGTA 1 cut(s) 867
Sse9I AATT 4 cut(s) 127, 357, 392, 514
SsiI CCGC 1 cut(s) 495
SspI AATATT 1 cut(s) 554
SspMI CTAG 4 cut(s) 6, 32, 540, 831
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 1 cut(s) 141
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 2 cut(s) 490, 704
TaiI ACGT 3 cut(s) 266, 573, 869
TaqI TCGA 3 cut(s) 72, 111, 190
TasI AATT 4 cut(s) 127, 357, 392, 514
TatI WGTACW 1 cut(s) 384
TauI GCSGC 1 cut(s) 498
TfiI GAWTC 2 cut(s) 257, 339
Tru1I TTAA 2 cut(s) 744, 846
Tru9I TTAA 2 cut(s) 744, 846
TscAI CASTG 2 cut(s) 18, 46
TseFI GTSAC 2 cut(s) 643, 833
TseI GCWGC 3 cut(s) 721, 731, 809
Tsp45I GTSAC 2 cut(s) 643, 833
TspDTI ATGAA 2 cut(s) 223, 793
TspGWI ACGGA 2 cut(s) 467, 483
TspRI CASTG 2 cut(s) 18, 46
Tth111I GACNNNGTC 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 656
XapI RAATTY 1 cut(s) 357
XmiI GTMKAC 2 cut(s) 111, 190
XspI CTAG 4 cut(s) 6, 32, 540, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.