MD04G1234400.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007620
Physical Location & Seq
Forward (+)
31322277 .. 31324748
2472 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1234400.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGCTAGCTCATTGGCCATTTTTGGTGTGGTCATGGCGTTGGGAATGTTGAGTTGTGGTGCTGAGGCTCGTGCCTTTTTTGTGTTTGGAGACTCACTGGTCGACAATGGCAACAATAACTACTTAGCCACCAGTGCTCGTGCCGACTCTCCTCCTTACGGCATTGACTATCCCACCCGTCGACCCACCGGCCGTTTCTCCAATGGCCTCACCATTCCTGACTATATCAGTCAGAAGCTTGGTGCAGAACCTACATTGCCATACTTGAGTCCACAGCTTACAGGGCAAAAGCTACTAGTTGGTGCAAACTTTGCCTCTGCTGGCATTGGAATTCTTAATGACACTGGGGTTCAGTTTGCAAATATAATCAGAATGCCATTGCAATTGCTAAACTTCCAACAATACCAGCAAAGGTTGTCTGCCCAAATCGGACCTCAACGGACCAAACAGCTCGTAAATCAAGCACTCGTCCTCCTCACCGTCGGTGGCAACGATTTTGTCAACAACTACTACCTAGTGCCTTTCTCCGCAAGATCTCGCCAGTTTTCTCTGCCAGACTATGTCAAATACCTCATTTCCGAGCTCCGAAAAATTCTCTTGAAAATGTATGATCTTGGAGCACGAAGGGTTTTGGTGACGGGGACAGGACCGATGGGCTGCGTGCCTGCTGAATTGGCCATGAGAAGCAGGAATGGAGAATGCGCAGCGGAGCTGCAACGAGCGTCGGCTCTGTACAACCCGCAACTCATTCAGATGCTGAGATCAGTCAACAGCCAACTCGGTTCTGATGTCTTTGTTGCTGCCAACGTACAGGAGACCCGCAATGACTTCATCAGGAACCCTCAAGCATTTGGATTTACTACATCAAAGGTTGCATGCTGTGGGCAAGGACCTTACAATGGCTTGGGGCTGTGCACAAGAGCTTCCAATTTGTGCCCGAACCGCGACCTATATGCTTTCTGGGATGCATTTCATCCGTCCGAAAAGGGGAACAGACTGATCGTTGAGAATATTTTGACAGGGGATGAAAAGTACATGTACCCCATGAATCTCAGCACCATCTTGGCTTTGGATTCCAGGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

362

Amino Acids

39.59

Weight (kDa)

9.02

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 27 - 338 7.5e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
Acc16I TGCGCA 1 cut(s) 703
AccI GTMKAC 2 cut(s) 102, 181
AccII CGCG 1 cut(s) 945
AciI CCGC 5 cut(s) 528, 707, 740, 820, 943
AcoI YGGCCR 3 cut(s) 15, 190, 675
AcsI RAATTY 2 cut(s) 330, 591
AfaI GTAC 4 cut(s) 734, 810, 1034, 1040
AfiI CCNNNNNNNGG 3 cut(s) 158, 899, 987
AflIII ACRYGT 1 cut(s) 1035
AgsI TTSAA 1 cut(s) 601
AhlI ACTAGT 1 cut(s) 295
AjnI CCWGG 1 cut(s) 1076
AluBI AGCT 8 cut(s) 9, 238, 277, 292, 451, 583, 712, 923
AluI AGCT 8 cut(s) 9, 238, 277, 292, 451, 583, 712, 923
Alw21I GWGCWC 4 cut(s) 139, 585, 622, 917
Alw26I GTCTC 2 cut(s) 84, 809
Alw44I GTGCAC 1 cut(s) 913
AlwNI CAGNNNCTG 1 cut(s) 757
AoxI GGCC 4 cut(s) 15, 190, 205, 675
ApaLI GTGCAC 1 cut(s) 913
ApeKI GCWGC 4 cut(s) 657, 704, 712, 800
ApoI RAATTY 2 cut(s) 330, 591
AspLEI GCGC 1 cut(s) 704
AspS9I GGNCC 5 cut(s) 431, 441, 647, 890, 1080
AsuHPI GGTGA 3 cut(s) 202, 469, 646
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 5 cut(s) 431, 441, 647, 890, 1080
BaeGI GKGCMC 2 cut(s) 917, 938
BalI TGGCCA 2 cut(s) 17, 677
BanII GRGCYC 1 cut(s) 585
BauI CACGAG 2 cut(s) 69, 138
Bbv12I GWGCWC 4 cut(s) 139, 585, 622, 917
BbvCI CCTCAGC 1 cut(s) 63
BbvI GCAGC 4 cut(s) 644, 699, 716, 787
BccI CCATC 2 cut(s) 646, 1067
BceAI ACGGC 2 cut(s) 175, 177
BcgI CGANNNNNNTGC 2 cut(s) 935, 969
BciT130I CCWGG 1 cut(s) 1078
BcoDI GTCTC 2 cut(s) 84, 809
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 3 cut(s) 6, 296, 515
BglII AGATCT 1 cut(s) 533
BisI GCNGC 4 cut(s) 658, 705, 713, 801
BlsI GCNGC 4 cut(s) 659, 706, 714, 802
Bme1390I CCNGG 1 cut(s) 1078
Bme18I GGWCC 5 cut(s) 431, 441, 647, 890, 1080
BmgT120I GGNCC 5 cut(s) 431, 441, 647, 890, 1080
BmiI GGNNCC 1 cut(s) 839
BmrFI CCNGG 1 cut(s) 1078
BmrI ACTGGG 1 cut(s) 354
BmsI GCATC 2 cut(s) 744, 955
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 354
Bpu10I CCTNAGC 1 cut(s) 63
BpuEI CTTGAG 2 cut(s) 286, 828
BsaI GGTCTC 1 cut(s) 809
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bsc4I CCNNNNNNNGG 3 cut(s) 158, 899, 987
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 4 cut(s) 102, 132, 349, 541
Bse3DI GCAATG 3 cut(s) 254, 377, 829
BseBI CCWGG 1 cut(s) 1078
BseGI GGATG 3 cut(s) 970, 973, 1030
BseLI CCNNNNNNNGG 3 cut(s) 158, 899, 987
BseMI GCAATG 3 cut(s) 254, 377, 829
BseMII CTCAG 3 cut(s) 54, 749, 1066
BseNI ACTGG 4 cut(s) 102, 132, 349, 541
BseRI GAGGAG 2 cut(s) 141, 464
BseSI GKGCMC 2 cut(s) 917, 938
BseX3I CGGCCG 1 cut(s) 190
BseXI GCAGC 4 cut(s) 644, 699, 716, 787
BsgI GTGCAG 1 cut(s) 264
Bsh1236I CGCG 1 cut(s) 945
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 4 cut(s) 17, 192, 207, 677
BsiEI CGRYCG 1 cut(s) 193
BsiHKAI GWGCWC 4 cut(s) 139, 585, 622, 917
BsiSI CCGG 1 cut(s) 189
BslFI GGGAC 1 cut(s) 655
BslI CCNNNNNNNGG 3 cut(s) 158, 899, 987
BsmAI GTCTC 2 cut(s) 84, 809
BsmFI GGGAC 1 cut(s) 655
BsmI GAATGC 2 cut(s) 378, 704
BsnI GGCC 4 cut(s) 17, 192, 207, 677
Bso31I GGTCTC 1 cut(s) 809
Bsp1286I GDGCHC 5 cut(s) 139, 585, 622, 917, 938
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 4 cut(s) 533, 610, 761, 999
BspACI CCGC 5 cut(s) 528, 707, 740, 820, 943
BspANI GGCC 4 cut(s) 17, 192, 207, 677
BspCNI CTCAG 3 cut(s) 55, 750, 1065
BspFNI CGCG 1 cut(s) 945
BspLI GGNNCC 1 cut(s) 839
BspOI GCTAGC 1 cut(s) 9
BspTNI GGTCTC 1 cut(s) 809
BsrDI GCAATG 3 cut(s) 254, 377, 829
BsrFI RCCGGY 1 cut(s) 188
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 4 cut(s) 102, 132, 349, 541
BssAI RCCGGY 1 cut(s) 188
BssMI GATC 4 cut(s) 533, 610, 761, 999
BssSI CACGAG 2 cut(s) 69, 138
Bst2BI CACGAG 2 cut(s) 69, 138
Bst2UI CCWGG 1 cut(s) 1078
Bst4CI ACNGT 1 cut(s) 481
BstAPI GCANNNNNTGC 1 cut(s) 311
BstAUI TGTACA 1 cut(s) 732
BstC8I GCNNGC 5 cut(s) 7, 322, 662, 666, 877
BstDEI CTNAG 4 cut(s) 63, 124, 758, 1052
BstF5I GGATG 3 cut(s) 970, 973, 1030
BstFNI CGCG 1 cut(s) 945
BstHHI GCGC 1 cut(s) 704
BstKTI GATC 4 cut(s) 536, 613, 764, 1002
BstMAI GTCTC 2 cut(s) 84, 809
BstMBI GATC 4 cut(s) 533, 610, 761, 999
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 5 cut(s) 134, 283, 311, 674, 942
BstNI CCWGG 1 cut(s) 1078
BstNSI RCATGY 2 cut(s) 879, 1039
BstSCI CCNGG 1 cut(s) 1076
BstSLI GKGCMC 2 cut(s) 917, 938
BstUI CGCG 1 cut(s) 945
BstV1I GCAGC 4 cut(s) 644, 699, 716, 787
BstX2I RGATCY 1 cut(s) 533
BstYI RGATCY 1 cut(s) 533
BstZI CGGCCG 1 cut(s) 190
BsuRI GGCC 4 cut(s) 17, 192, 207, 677
BtsCI GGATG 3 cut(s) 970, 973, 1030
BtsIMutI CAGTG 3 cut(s) 95, 139, 342
Cac8I GCNNGC 5 cut(s) 7, 322, 662, 666, 877
CaiI CAGNNNCTG 1 cut(s) 757
CfoI GCGC 1 cut(s) 704
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 5 cut(s) 431, 441, 647, 890, 1080
CseI GACGC 1 cut(s) 711
Csp6I GTAC 4 cut(s) 733, 809, 1033, 1039
CviAII CATG 5 cut(s) 34, 679, 876, 1036, 1045
CviQI GTAC 4 cut(s) 733, 809, 1033, 1039
DdeI CTNAG 4 cut(s) 63, 124, 758, 1052
DpnI GATC 4 cut(s) 535, 612, 763, 1001
DpnII GATC 4 cut(s) 533, 610, 761, 999
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
EaeI YGGCCR 3 cut(s) 15, 190, 675
EagI CGGCCG 1 cut(s) 190
Ecl136II GAGCTC 1 cut(s) 583
EclXI CGGCCG 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 585
Eco31I GGTCTC 1 cut(s) 809
Eco47I GGWCC 5 cut(s) 431, 441, 647, 890, 1080
Eco52I CGGCCG 1 cut(s) 190
Eco53kI GAGCTC 1 cut(s) 583
EcoICRI GAGCTC 1 cut(s) 583
EcoO109I RGGNCCY 2 cut(s) 890, 1080
EcoRI GAATTC 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 1076
EcoT22I ATGCAT 1 cut(s) 970
EcoT38I GRGCYC 1 cut(s) 585
FaeI CATG 5 cut(s) 37, 682, 879, 1039, 1048
FaqI GGGAC 1 cut(s) 655
FatI CATG 5 cut(s) 33, 678, 875, 1035, 1044
FauI CCCGC 2 cut(s) 747, 827
FblI GTMKAC 2 cut(s) 102, 181
Fnu4HI GCNGC 4 cut(s) 658, 705, 713, 801
FokI GGATG 3 cut(s) 960, 977, 1037
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 4 cut(s) 658, 705, 713, 801
FspBI CTAG 3 cut(s) 6, 296, 515
FspI TGCGCA 1 cut(s) 703
GlaI GCGC 1 cut(s) 703
GluI GCNGC 4 cut(s) 658, 705, 713, 801
HaeIII GGCC 4 cut(s) 17, 192, 207, 677
HapII CCGG 1 cut(s) 189
HgaI GACGC 1 cut(s) 711
HhaI GCGC 1 cut(s) 704
Hin1II CATG 5 cut(s) 37, 682, 879, 1039, 1048
Hin6I GCGC 1 cut(s) 702
HinP1I GCGC 1 cut(s) 702
HincII GTYRAC 4 cut(s) 103, 182, 502, 769
HindII GTYRAC 4 cut(s) 103, 182, 502, 769
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 5 cut(s) 92, 146, 268, 1048, 1073
HpaII CCGG 1 cut(s) 189
HphI GGTGA 3 cut(s) 202, 469, 646
Hpy166II GTNNAC 6 cut(s) 103, 182, 272, 502, 769, 915
Hpy188I TCNGA 8 cut(s) 234, 371, 431, 580, 587, 753, 787, 982
Hpy188III TCNNGA 3 cut(s) 218, 598, 835
Hpy8I GTNNAC 6 cut(s) 103, 182, 272, 502, 769, 915
Hpy99I CGWCG 3 cut(s) 183, 485, 727
HpyAV CCTTC 1 cut(s) 618
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4IV ACGT 1 cut(s) 807
HpyCH4V TGCA 8 cut(s) 245, 305, 359, 382, 715, 875, 915, 968
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 283, 311, 674, 942
HpyF3I CTNAG 4 cut(s) 63, 124, 758, 1052
HpySE526I ACGT 1 cut(s) 807
Hsp92II CATG 5 cut(s) 37, 682, 879, 1039, 1048
HspAI GCGC 1 cut(s) 702
Kzo9I GATC 4 cut(s) 533, 610, 761, 999
LmnI GCTCC 3 cut(s) 588, 617, 709
Lsp1109I GCAGC 4 cut(s) 644, 699, 716, 787
LweI GCATC 2 cut(s) 744, 955
MaeI CTAG 3 cut(s) 6, 296, 515
MaeII ACGT 1 cut(s) 807
MaeIII GTNAC 1 cut(s) 634
MalI GATC 4 cut(s) 535, 612, 763, 1001
MboI GATC 4 cut(s) 533, 610, 761, 999
MfeI CAATTG 1 cut(s) 383
MflI RGATCY 1 cut(s) 533
MhlI GDGCHC 5 cut(s) 139, 585, 622, 917, 938
MlsI TGGCCA 2 cut(s) 17, 677
MluCI AATT 5 cut(s) 330, 383, 591, 671, 928
MluNI TGGCCA 2 cut(s) 17, 677
MlyI GAGTC 3 cut(s) 86, 140, 277
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 9 cut(s) 58, 162, 218, 325, 444, 482, 485, 581, 852
Mox20I TGGCCA 2 cut(s) 17, 677
Mph1103I ATGCAT 1 cut(s) 970
MscI TGGCCA 2 cut(s) 17, 677
MseI TTAA 1 cut(s) 336
MslI CAYNNNNRTG 1 cut(s) 752
Msp20I TGGCCA 2 cut(s) 17, 677
MspA1I CMGCKG 1 cut(s) 707
MspI CCGG 1 cut(s) 189
MspR9I CCNGG 1 cut(s) 1078
MunI CAATTG 1 cut(s) 383
Mva1269I GAATGC 2 cut(s) 378, 704
MvaI CCWGG 1 cut(s) 1078
MvnI CGCG 1 cut(s) 945
MwoI GCNNNNNNNGC 5 cut(s) 134, 283, 311, 674, 942
NdeII GATC 4 cut(s) 533, 610, 761, 999
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 5 cut(s) 37, 682, 879, 1039, 1048
NlaIV GGNNCC 1 cut(s) 839
NmuCI GTSAC 1 cut(s) 634
NsbI TGCGCA 1 cut(s) 703
NsiI ATGCAT 1 cut(s) 970
NspI RCATGY 2 cut(s) 879, 1039
PaeI GCATGC 1 cut(s) 879
PciI ACATGT 1 cut(s) 1035
PcsI WCGNNNNNNNCGW 1 cut(s) 489
PctI GAATGC 2 cut(s) 378, 704
PfeI GAWTC 2 cut(s) 1048, 1073
PflFI GACNNNGTC 1 cut(s) 560
PfoI TCCNGGA 1 cut(s) 1076
PkrI GCNGC 4 cut(s) 659, 706, 714, 802
PleI GAGTC 3 cut(s) 86, 140, 276
PpsI GAGTC 3 cut(s) 86, 140, 276
PpuMI RGGWCCY 2 cut(s) 890, 1080
PscI ACATGT 1 cut(s) 1035
Psp124BI GAGCTC 1 cut(s) 585
Psp5II RGGWCCY 2 cut(s) 890, 1080
Psp6I CCWGG 1 cut(s) 1076
PspGI CCWGG 1 cut(s) 1076
PspN4I GGNNCC 1 cut(s) 839
PspPI GGNCC 5 cut(s) 431, 441, 647, 890, 1080
PspPPI RGGWCCY 2 cut(s) 890, 1080
PstNI CAGNNNCTG 1 cut(s) 757
PsuI RGATCY 1 cut(s) 533
PsyI GACNNNGTC 1 cut(s) 560
RsaI GTAC 4 cut(s) 734, 810, 1034, 1040
RsaNI GTAC 4 cut(s) 733, 809, 1033, 1039
RseI CAYNNNNRTG 1 cut(s) 752
SacI GAGCTC 1 cut(s) 585
SalI GTCGAC 2 cut(s) 101, 180
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 4 cut(s) 658, 705, 713, 801
Sau3AI GATC 4 cut(s) 533, 610, 761, 999
Sau96I GGNCC 5 cut(s) 431, 441, 647, 890, 1080
SchI GAGTC 3 cut(s) 86, 140, 277
ScrFI CCNGG 1 cut(s) 1078
SduI GDGCHC 5 cut(s) 139, 585, 622, 917, 938
SfaNI GCATC 2 cut(s) 744, 955
SinI GGWCC 5 cut(s) 431, 441, 647, 890, 1080
SmiMI CAYNNNNRTG 1 cut(s) 752
SmlI CTYRAG 2 cut(s) 265, 843
SmoI CTYRAG 2 cut(s) 265, 843
SpeI ACTAGT 1 cut(s) 295
SphI GCATGC 1 cut(s) 879
Sse9I AATT 5 cut(s) 330, 383, 591, 671, 928
SsiI CCGC 5 cut(s) 528, 707, 740, 820, 943
SspI AATATT 1 cut(s) 1012
SspMI CTAG 3 cut(s) 6, 296, 515
SstI GAGCTC 1 cut(s) 585
StyD4I CCNGG 1 cut(s) 1076
TaaI ACNGT 1 cut(s) 481
TaiI ACGT 1 cut(s) 810
TaqI TCGA 2 cut(s) 102, 181
TaqII GACCGA 1 cut(s) 664
TasI AATT 5 cut(s) 330, 383, 591, 671, 928
TatI WGTACW 2 cut(s) 732, 1032
TfiI GAWTC 2 cut(s) 1048, 1073
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 3 cut(s) 102, 139, 349
TseFI GTSAC 1 cut(s) 634
TseI GCWGC 4 cut(s) 657, 704, 712, 800
Tsp45I GTSAC 1 cut(s) 634
TspDTI ATGAA 4 cut(s) 820, 962, 1041, 1061
TspGWI ACGGA 2 cut(s) 454, 966
TspRI CASTG 3 cut(s) 102, 139, 349
Tth111I GACNNNGTC 1 cut(s) 560
VneI GTGCAC 1 cut(s) 913
VpaK11BI GGWCC 5 cut(s) 431, 441, 647, 890, 1080
XapI RAATTY 2 cut(s) 330, 591
XceI RCATGY 2 cut(s) 879, 1039
XcmI CCANNNNNNNNNTGG 2 cut(s) 25, 1066
XmiI GTMKAC 2 cut(s) 102, 181
XspI CTAG 3 cut(s) 6, 296, 515
Zsp2I ATGCAT 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.