Rmu_sc0003116.1_g000014

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003116.1
OG0007620
Physical Location & Seq
Forward (+)
71881 .. 73668
1788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003116.1_g000014.1.cds

Sequence Viewer

Length: 1095 bp
atggctagctcactggtgctgattttcagtctggtcctcactgcattggaaagcttcggcacccagcaggtcgaggctcgtgcctttttcgtgttcggagactccttggtcgacaatggcaacaacaattacttgctaacccaggctcgtgccgacgcttatccttatggcattgactatccaactggtcgaccaacgggccgtttctccaatggctacaacatccctgacttcatcagtcaagcacttggtgcagaatccacgttgccttacttgagtccagagctcaccggacaaaggctacttgttggtgccaactttgcttctgctggcattggaatcctcaatgacaccggaattcagtttgtaaacataatcagaatgtacagacaatttgagtattttcaagaataccagcaaagagtgagtgcccttatcggatctgaacgggcggagcaacttgtaagcggagcacttattctcatgactgttggcggcaacgactttgtcaacaattactacttggttccctactctgctagatctcgtcaatattctcttccagattatgtcgaatacctcatttctgagttcagaaaacttcttttgaggctatacgatcttggagggcgtagggttttggtgacaggtacaggaccattaggctgtgttccagctgagttggccacgagaagtacgaacggtcaatgctcagccgagctgcaaagagctgcaggtctctttaatccgcaacttactcagatgctaactggactcaacagggaactgggtgccgatatcttcattgctgctaacactcagcagagtgctagtgacttcgtggttaaccctcaagcatatggatttactacagcgaaaattgcctgctgcgggcaaggaccttacaatgggctgggattgtgcactggattgtccaatttgtgcccgaacagagacgtatatgctttctgggattcatttcatccatcagaaagggcaaaccggtacattgttcagaatattctgaccggcactactgaatacatgaaccccatgaacctcagcaccattttggctttggactccaggacctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

364

Amino Acids

39.97

Weight (kDa)

5.07

Isoelectric Point (pI)

31.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
Acc36I ACCTGC 2 cut(s) 58, 725
AccB1I GGYRCC 3 cut(s) 59, 311, 791
AccI GTMKAC 2 cut(s) 111, 190
AciI CCGC 5 cut(s) 452, 468, 495, 749, 891
AclWI GGATC 1 cut(s) 448
AcoI YGGCCR 1 cut(s) 684
AcsI RAATTY 1 cut(s) 357
AdeI CACNNNGTG 1 cut(s) 251
AfaI GTAC 4 cut(s) 386, 652, 697, 1007
AfiI CCNNNNNNNGG 3 cut(s) 297, 891, 908
AgeI ACCGGT 1 cut(s) 1002
AgsI TTSAA 1 cut(s) 407
AjnI CCWGG 2 cut(s) 141, 1085
AluBI AGCT 6 cut(s) 9, 54, 286, 677, 721, 731
AluI AGCT 6 cut(s) 9, 54, 286, 677, 721, 731
Alw21I GWGCWC 3 cut(s) 288, 475, 926
Alw26I GTCTC 3 cut(s) 93, 743, 948
Alw44I GTGCAC 1 cut(s) 922
AlwI GGATC 1 cut(s) 448
AoxI GGCC 2 cut(s) 199, 684
ApaLI GTGCAC 1 cut(s) 922
ApeKI GCWGC 4 cut(s) 721, 731, 809, 888
ApoI RAATTY 1 cut(s) 357
AsiGI ACCGGT 1 cut(s) 1002
AspS9I GGNCC 5 cut(s) 34, 199, 656, 899, 1089
AsuHPI GGTGA 2 cut(s) 280, 655
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 4 cut(s) 34, 656, 899, 1089
BaeGI GKGCMC 3 cut(s) 433, 926, 947
BalI TGGCCA 1 cut(s) 686
BanI GGYRCC 3 cut(s) 59, 311, 791
BanII GRGCYC 1 cut(s) 288
BauI CACGAG 3 cut(s) 78, 147, 688
Bbv12I GWGCWC 3 cut(s) 288, 475, 926
BbvCI CCTCAGC 1 cut(s) 1061
BbvI GCAGC 4 cut(s) 708, 718, 796, 875
BccI CCATC 1 cut(s) 994
BceAI ACGGC 1 cut(s) 186
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 2 cut(s) 143, 1087
BcoDI GTCTC 3 cut(s) 93, 743, 948
BfaI CTAG 4 cut(s) 6, 540, 831, 1093
BfmI CTRYAG 2 cut(s) 732, 870
BfuAI ACCTGC 2 cut(s) 58, 725
BglII AGATCT 1 cut(s) 542
BisI GCNGC 5 cut(s) 496, 722, 732, 810, 889
BlpI GCTNAGC 1 cut(s) 712
BlsI GCNGC 5 cut(s) 497, 723, 733, 811, 890
Bme1390I CCNGG 2 cut(s) 143, 1087
Bme18I GGWCC 4 cut(s) 34, 656, 899, 1089
BmgT120I GGNCC 5 cut(s) 34, 199, 656, 899, 1089
BmiI GGNNCC 4 cut(s) 61, 313, 528, 793
BmrFI CCNGG 2 cut(s) 143, 1087
BmrI ACTGGG 1 cut(s) 797
BmsI GCATC 1 cut(s) 753
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 797
BpmI CTGGAG 1 cut(s) 1069
Bpu10I CCTNAGC 1 cut(s) 1061
Bpu1102I GCTNAGC 1 cut(s) 712
BpuEI CTTGAG 2 cut(s) 295, 837
BsaI GGTCTC 1 cut(s) 743
BsaJI CCNNGG 2 cut(s) 105, 141
BsaWI WCCGGW 3 cut(s) 290, 353, 1002
Bsc4I CCNNNNNNNGG 3 cut(s) 297, 891, 908
Bse118I RCCGGY 2 cut(s) 1002, 1028
Bse1I ACTGG 5 cut(s) 18, 190, 775, 792, 931
Bse3DI GCAATG 1 cut(s) 804
BseBI CCWGG 2 cut(s) 143, 1087
BseDI CCNNGG 2 cut(s) 105, 141
BseGI GGATG 2 cut(s) 222, 982
BseLI CCNNNNNNNGG 3 cut(s) 297, 891, 908
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 6 cut(s) 579, 669, 726, 773, 833, 1075
BseNI ACTGG 5 cut(s) 18, 190, 775, 792, 931
BseSI GKGCMC 3 cut(s) 433, 926, 947
BseXI GCAGC 4 cut(s) 708, 718, 796, 875
BseYI CCCAGC 2 cut(s) 63, 913
BsgI GTGCAG 1 cut(s) 273
BshFI GGCC 2 cut(s) 201, 686
BshNI GGYRCC 3 cut(s) 59, 311, 791
BshTI ACCGGT 1 cut(s) 1002
BsiHKAI GWGCWC 3 cut(s) 288, 475, 926
BsiSI CCGG 4 cut(s) 291, 354, 1003, 1029
BslI CCNNNNNNNGG 3 cut(s) 297, 891, 908
BsmAI GTCTC 3 cut(s) 93, 743, 948
BsmBI CGTCTC 1 cut(s) 948
BsnI GGCC 2 cut(s) 201, 686
Bso31I GGTCTC 1 cut(s) 743
Bsp1286I GDGCHC 5 cut(s) 288, 433, 475, 926, 947
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 3 cut(s) 440, 542, 619
Bsp1720I GCTNAGC 1 cut(s) 712
BspACI CCGC 5 cut(s) 452, 468, 495, 749, 891
BspANI GGCC 2 cut(s) 201, 686
BspCNI CTCAG 6 cut(s) 580, 670, 725, 772, 832, 1074
BspHI TCATGA 1 cut(s) 483
BspLI GGNNCC 4 cut(s) 61, 313, 528, 793
BspMAI CTGCAG 1 cut(s) 736
BspMI ACCTGC 2 cut(s) 58, 725
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 448
BspT107I GGYRCC 3 cut(s) 59, 311, 791
BspTNI GGTCTC 1 cut(s) 743
BsrDI GCAATG 1 cut(s) 804
BsrFI RCCGGY 2 cut(s) 1002, 1028
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 5 cut(s) 18, 190, 775, 792, 931
BssAI RCCGGY 2 cut(s) 1002, 1028
BssECI CCNNGG 2 cut(s) 105, 141
BssMI GATC 3 cut(s) 440, 542, 619
BssSI CACGAG 3 cut(s) 78, 147, 688
BssT1I CCWWGG 1 cut(s) 105
Bst2BI CACGAG 3 cut(s) 78, 147, 688
Bst2UI CCWGG 2 cut(s) 143, 1087
Bst4CI ACNGT 2 cut(s) 490, 704
Bst6I CTCTTC 1 cut(s) 564
BstAPI GCANNNNNTGC 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 384
BstC8I GCNNGC 4 cut(s) 7, 331, 886, 893
BstDEI CTNAG 6 cut(s) 588, 678, 712, 759, 819, 1061
BstF5I GGATG 2 cut(s) 222, 982
BstKTI GATC 3 cut(s) 443, 545, 622
BstMAI GTCTC 3 cut(s) 93, 743, 948
BstMBI GATC 3 cut(s) 440, 542, 619
BstMWI GCNNNNNNNGC 4 cut(s) 251, 320, 683, 881
BstNI CCWGG 2 cut(s) 143, 1087
BstSCI CCNGG 2 cut(s) 141, 1085
BstSFI CTRYAG 2 cut(s) 732, 870
BstSLI GKGCMC 3 cut(s) 433, 926, 947
BstV1I GCAGC 4 cut(s) 708, 718, 796, 875
BstX2I RGATCY 2 cut(s) 440, 542
BstYI RGATCY 2 cut(s) 440, 542
BsuRI GGCC 2 cut(s) 201, 686
BtsCI GGATG 2 cut(s) 222, 982
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 3 cut(s) 11, 39, 924
BveI ACCTGC 2 cut(s) 58, 725
Cac8I GCNNGC 4 cut(s) 7, 331, 886, 893
CciI TCATGA 1 cut(s) 483
Cfr10I RCCGGY 2 cut(s) 1002, 1028
Cfr13I GGNCC 5 cut(s) 34, 199, 656, 899, 1089
CseI GACGC 1 cut(s) 164
Csp6I GTAC 4 cut(s) 385, 651, 696, 1006
CspAI ACCGGT 1 cut(s) 1002
CviAII CATG 3 cut(s) 484, 1045, 1054
CviQI GTAC 4 cut(s) 385, 651, 696, 1006
DdeI CTNAG 6 cut(s) 588, 678, 712, 759, 819, 1061
DpnI GATC 3 cut(s) 442, 544, 621
DpnII GATC 3 cut(s) 440, 542, 619
DraIII CACNNNGTG 1 cut(s) 251
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 1 cut(s) 684
Eam1104I CTCTTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 564
EciI GGCGGA 1 cut(s) 467
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 743
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 4 cut(s) 34, 656, 899, 1089
Eco53kI GAGCTC 1 cut(s) 286
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 2 cut(s) 899, 1089
EcoRI GAATTC 1 cut(s) 357
EcoRII CCWGG 2 cut(s) 141, 1085
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 1 cut(s) 105
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 105
Esp3I CGTCTC 1 cut(s) 948
FaeI CATG 3 cut(s) 487, 1048, 1057
FatI CATG 3 cut(s) 483, 1044, 1053
FauI CCCGC 1 cut(s) 884
FauNDI CATATG 1 cut(s) 859
FblI GTMKAC 2 cut(s) 111, 190
Fnu4HI GCNGC 5 cut(s) 496, 722, 732, 810, 889
FokI GGATG 2 cut(s) 209, 969
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 5 cut(s) 496, 722, 732, 810, 889
FspBI CTAG 4 cut(s) 6, 540, 831, 1093
GluI GCNGC 5 cut(s) 496, 722, 732, 810, 889
GsaI CCCAGC 2 cut(s) 67, 917
GsuI CTGGAG 1 cut(s) 1069
HaeIII GGCC 2 cut(s) 201, 686
HapII CCGG 4 cut(s) 291, 354, 1003, 1029
HgaI GACGC 1 cut(s) 164
Hin1II CATG 3 cut(s) 487, 1048, 1057
HincII GTYRAC 4 cut(s) 112, 191, 511, 847
HindII GTYRAC 4 cut(s) 112, 191, 511, 847
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 7 cut(s) 101, 257, 277, 339, 774, 974, 1082
HpaI GTTAAC 1 cut(s) 847
HpaII CCGG 4 cut(s) 291, 354, 1003, 1029
HphI GGTGA 2 cut(s) 280, 655
Hpy166II GTNNAC 6 cut(s) 112, 191, 370, 511, 847, 924
Hpy188III TCNNGA 4 cut(s) 281, 407, 484, 563
Hpy8I GTNNAC 6 cut(s) 112, 191, 370, 511, 847, 924
Hpy99I CGWCG 1 cut(s) 158
HpyCH4III ACNGT 2 cut(s) 490, 704
HpyCH4IV ACGT 2 cut(s) 263, 957
HpyCH4V TGCA 5 cut(s) 44, 254, 724, 734, 924
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 320, 683, 881
HpyF3I CTNAG 6 cut(s) 588, 678, 712, 759, 819, 1061
HpySE526I ACGT 2 cut(s) 263, 957
Hsp92II CATG 3 cut(s) 487, 1048, 1057
KspAI GTTAAC 1 cut(s) 847
Kzo9I GATC 3 cut(s) 440, 542, 619
LmnI GCTCC 2 cut(s) 454, 470
Lsp1109I GCAGC 4 cut(s) 708, 718, 796, 875
LweI GCATC 1 cut(s) 753
MaeI CTAG 4 cut(s) 6, 540, 831, 1093
MaeII ACGT 2 cut(s) 263, 957
MaeIII GTNAC 2 cut(s) 643, 833
MalI GATC 3 cut(s) 442, 544, 621
MboI GATC 3 cut(s) 440, 542, 619
MboII GAAGA 2 cut(s) 551, 793
MflI RGATCY 2 cut(s) 440, 542
MhlI GDGCHC 5 cut(s) 288, 433, 475, 926, 947
MlsI TGGCCA 1 cut(s) 686
MluCI AATT 6 cut(s) 127, 357, 392, 514, 879, 937
MluNI TGGCCA 1 cut(s) 686
MlyI GAGTC 4 cut(s) 95, 286, 768, 1076
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 8 cut(s) 47, 67, 353, 590, 603, 620, 861, 1070
Mox20I TGGCCA 1 cut(s) 686
MscI TGGCCA 1 cut(s) 686
MseI TTAA 2 cut(s) 744, 846
Msp20I TGGCCA 1 cut(s) 686
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 4 cut(s) 291, 354, 1003, 1029
MspR9I CCNGG 2 cut(s) 143, 1087
MvaI CCWGG 2 cut(s) 143, 1087
MwoI GCNNNNNNNGC 4 cut(s) 251, 320, 683, 881
NdeI CATATG 1 cut(s) 859
NdeII GATC 3 cut(s) 440, 542, 619
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 3 cut(s) 487, 1048, 1057
NlaIV GGNNCC 4 cut(s) 61, 313, 528, 793
NmeAIII GCCGAG 1 cut(s) 742
NmuCI GTSAC 2 cut(s) 643, 833
PagI TCATGA 1 cut(s) 483
PcsI WCGNNNNNNNCGW 1 cut(s) 695
PfeI GAWTC 3 cut(s) 257, 339, 974
PflFI GACNNNGTC 1 cut(s) 506
PfoI TCCNGGA 1 cut(s) 1085
PinAI ACCGGT 1 cut(s) 1002
PkrI GCNGC 5 cut(s) 497, 723, 733, 811, 890
PleI GAGTC 4 cut(s) 95, 285, 768, 1076
PpsI GAGTC 4 cut(s) 95, 285, 768, 1076
PpuMI RGGWCCY 2 cut(s) 899, 1089
Psp124BI GAGCTC 1 cut(s) 288
Psp5II RGGWCCY 2 cut(s) 899, 1089
Psp6I CCWGG 2 cut(s) 141, 1085
PspFI CCCAGC 2 cut(s) 63, 913
PspGI CCWGG 2 cut(s) 141, 1085
PspN4I GGNNCC 4 cut(s) 61, 313, 528, 793
PspPI GGNCC 5 cut(s) 34, 199, 656, 899, 1089
PspPPI RGGWCCY 2 cut(s) 899, 1089
PstI CTGCAG 1 cut(s) 736
PsuI RGATCY 2 cut(s) 440, 542
PsyI GACNNNGTC 1 cut(s) 506
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 4 cut(s) 386, 652, 697, 1007
RsaNI GTAC 4 cut(s) 385, 651, 696, 1006
SacI GAGCTC 1 cut(s) 288
SalI GTCGAC 2 cut(s) 110, 189
SaqAI TTAA 2 cut(s) 744, 846
SatI GCNGC 5 cut(s) 496, 722, 732, 810, 889
Sau3AI GATC 3 cut(s) 440, 542, 619
Sau96I GGNCC 5 cut(s) 34, 199, 656, 899, 1089
SchI GAGTC 4 cut(s) 95, 286, 768, 1076
ScrFI CCNGG 2 cut(s) 143, 1087
SduI GDGCHC 5 cut(s) 288, 433, 475, 926, 947
SfaNI GCATC 1 cut(s) 753
SfcI CTRYAG 2 cut(s) 732, 870
SinI GGWCC 4 cut(s) 34, 656, 899, 1089
SmlI CTYRAG 2 cut(s) 274, 852
SmoI CTYRAG 2 cut(s) 274, 852
Sse9I AATT 6 cut(s) 127, 357, 392, 514, 879, 937
SsiI CCGC 5 cut(s) 452, 468, 495, 749, 891
SspI AATATT 2 cut(s) 554, 1021
SspMI CTAG 4 cut(s) 6, 540, 831, 1093
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 141, 1085
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 2 cut(s) 490, 704
TaiI ACGT 2 cut(s) 266, 960
TaqI TCGA 4 cut(s) 72, 111, 190, 573
TasI AATT 6 cut(s) 127, 357, 392, 514, 879, 937
TatI WGTACW 1 cut(s) 384
TauI GCSGC 1 cut(s) 498
TfiI GAWTC 3 cut(s) 257, 339, 974
Tru1I TTAA 2 cut(s) 744, 846
Tru9I TTAA 2 cut(s) 744, 846
TscAI CASTG 3 cut(s) 18, 46, 931
TseFI GTSAC 2 cut(s) 643, 833
TseI GCWGC 4 cut(s) 721, 731, 809, 888
Tsp45I GTSAC 2 cut(s) 643, 833
TspDTI ATGAA 6 cut(s) 223, 793, 966, 971, 1061, 1070
TspRI CASTG 3 cut(s) 18, 46, 931
Tth111I GACNNNGTC 1 cut(s) 506
VneI GTGCAC 1 cut(s) 922
VpaK11BI GGWCC 4 cut(s) 34, 656, 899, 1089
XapI RAATTY 1 cut(s) 357
XcmI CCANNNNNNNNNTGG 1 cut(s) 1075
XmiI GTMKAC 2 cut(s) 111, 190
XspI CTAG 4 cut(s) 6, 540, 831, 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.