Rh3AG015800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0007620
Physical Location & Seq
Reverse (-)
986825 .. 989339
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG015800.1

Sequence Viewer

Length: 810 bp
ATGGAAGCGTTTTTATTAGAAGTCGTAGAGACAAAAACGTTTATGGAAGTGCTTATGGAAATTCTTAAAACAATTTTGCAGGTAAACATAATCAGAATGTACAGACAATTTGAGTATTTTCAAGAATACCAGCAAAGAGTGAGTGCCCTTATCGGATCTGAACAGACGGAGCGACTTGTAAACGGAGCACTTATTCTCATGACTGTTGGCGGCAACGACTTTGTCAACAATTACTACTTGGTTCCCTACTCTGCTAGATCTCGTCAATATTCCCTTCCAGATTACGTCAAATACCTCATTTCTGAGTTCAGAAACCTTCTTTTGAGGCTATACGATCTTGGAGGGCGTAGGGTTTTGGTGACAGGTACAGGACCATTAGGCTGTGTTCCAGCTGAGTTGGCCACGAGAAGTTCTAACGGTCAATGCTCAGCCGAGCTGCAAAGAGCTGCAGGTCTCTTTAATCCACAACTTACTCAGATGCTAAGTGGACTCAACAGGGAACTGGGTGCCGATATCTTCATTGCTGCTAACACTCAGCAGAGTGCTAGTGACTTCGTGGTTAACCCTCAAGCATATGGATTTACTACAGCGAAAATTGCCTGCTGCGGGCAAGGACCTTACAATGGGCTGGGATTGTGCACTGGATTGTCCAATTTGTGCCCGAACAGAGACCTATATGCTTTCTGGGATTCATTTCATCCATCAGAAAAGGCAAATCGGTACATTGTTCAGAATATTCTGTCCGGCACTACTGAATACATGAACCCCATGAACCTCAGCACCATTTTGGCTTTGGACTCCAGGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

269

Amino Acids

30.09

Weight (kDa)

5.56

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 27 - 244 8.3e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 70, 440
AccB1I GGYRCC 1 cut(s) 506
AciI CCGC 2 cut(s) 210, 606
AclI AACGTT 1 cut(s) 38
AclWI GGATC 1 cut(s) 163
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 1 cut(s) 60
AfaI GTAC 3 cut(s) 101, 367, 722
AfiI CCNNNNNNNGG 2 cut(s) 606, 623
AgsI TTSAA 1 cut(s) 122
AjnI CCWGG 1 cut(s) 800
AluBI AGCT 3 cut(s) 392, 436, 446
AluI AGCT 3 cut(s) 392, 436, 446
Alw21I GWGCWC 2 cut(s) 190, 641
Alw26I GTCTC 3 cut(s) 23, 458, 663
Alw44I GTGCAC 1 cut(s) 637
AlwI GGATC 1 cut(s) 163
AoxI GGCC 1 cut(s) 399
ApaLI GTGCAC 1 cut(s) 637
ApeKI GCWGC 4 cut(s) 436, 446, 524, 603
ApoI RAATTY 1 cut(s) 60
AspS9I GGNCC 3 cut(s) 371, 614, 804
AsuHPI GGTGA 1 cut(s) 370
AvaII GGWCC 3 cut(s) 371, 614, 804
BaeGI GKGCMC 3 cut(s) 148, 641, 662
BalI TGGCCA 1 cut(s) 401
BanI GGYRCC 1 cut(s) 506
BauI CACGAG 1 cut(s) 403
Bbv12I GWGCWC 2 cut(s) 190, 641
BbvCI CCTCAGC 1 cut(s) 776
BbvI GCAGC 4 cut(s) 423, 433, 511, 590
BccI CCATC 1 cut(s) 709
BciT130I CCWGG 1 cut(s) 802
BcoDI GTCTC 3 cut(s) 23, 458, 663
BfaI CTAG 3 cut(s) 255, 546, 808
BfmI CTRYAG 2 cut(s) 447, 585
BfuAI ACCTGC 2 cut(s) 70, 440
BglII AGATCT 1 cut(s) 257
BisI GCNGC 5 cut(s) 211, 437, 447, 525, 604
BlpI GCTNAGC 1 cut(s) 427
BlsI GCNGC 5 cut(s) 212, 438, 448, 526, 605
Bme1390I CCNGG 1 cut(s) 802
Bme18I GGWCC 3 cut(s) 371, 614, 804
BmgT120I GGNCC 3 cut(s) 371, 614, 804
BmiI GGNNCC 2 cut(s) 243, 508
BmrFI CCNGG 1 cut(s) 802
BmrI ACTGGG 1 cut(s) 512
BmsI GCATC 1 cut(s) 468
BmuI ACTGGG 1 cut(s) 512
BpmI CTGGAG 1 cut(s) 784
Bpu10I CCTNAGC 1 cut(s) 776
Bpu1102I GCTNAGC 1 cut(s) 427
BpuEI CTTGAG 1 cut(s) 552
BsaI GGTCTC 2 cut(s) 458, 663
Bsc4I CCNNNNNNNGG 2 cut(s) 606, 623
Bse1I ACTGG 2 cut(s) 507, 646
Bse3DI GCAATG 1 cut(s) 519
BseBI CCWGG 1 cut(s) 802
BseGI GGATG 1 cut(s) 697
BseLI CCNNNNNNNGG 2 cut(s) 606, 623
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 6 cut(s) 294, 384, 441, 488, 548, 790
BseNI ACTGG 2 cut(s) 507, 646
BseSI GKGCMC 3 cut(s) 148, 641, 662
BseXI GCAGC 4 cut(s) 423, 433, 511, 590
BseYI CCCAGC 1 cut(s) 628
BshFI GGCC 1 cut(s) 401
BshNI GGYRCC 1 cut(s) 506
BsiHKAI GWGCWC 2 cut(s) 190, 641
BsiSI CCGG 1 cut(s) 744
BslI CCNNNNNNNGG 2 cut(s) 606, 623
BsmAI GTCTC 3 cut(s) 23, 458, 663
BsnI GGCC 1 cut(s) 401
Bso31I GGTCTC 2 cut(s) 458, 663
Bsp1286I GDGCHC 4 cut(s) 148, 190, 641, 662
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 3 cut(s) 155, 257, 334
Bsp1720I GCTNAGC 1 cut(s) 427
BspACI CCGC 2 cut(s) 210, 606
BspANI GGCC 1 cut(s) 401
BspCNI CTCAG 6 cut(s) 295, 385, 440, 487, 547, 789
BspHI TCATGA 1 cut(s) 198
BspLI GGNNCC 2 cut(s) 243, 508
BspMAI CTGCAG 1 cut(s) 451
BspMI ACCTGC 2 cut(s) 70, 440
BspPI GGATC 1 cut(s) 163
BspT107I GGYRCC 1 cut(s) 506
BspTNI GGTCTC 2 cut(s) 458, 663
BsrDI GCAATG 1 cut(s) 519
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 2 cut(s) 507, 646
BssMI GATC 3 cut(s) 155, 257, 334
BssSI CACGAG 1 cut(s) 403
Bst2BI CACGAG 1 cut(s) 403
Bst2UI CCWGG 1 cut(s) 802
Bst4CI ACNGT 2 cut(s) 205, 419
BstAUI TGTACA 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 601, 608
BstDEI CTNAG 7 cut(s) 303, 393, 427, 474, 482, 534, 776
BstF5I GGATG 1 cut(s) 697
BstKTI GATC 3 cut(s) 158, 260, 337
BstMAI GTCTC 3 cut(s) 23, 458, 663
BstMBI GATC 3 cut(s) 155, 257, 334
BstMWI GCNNNNNNNGC 2 cut(s) 398, 596
BstNI CCWGG 1 cut(s) 802
BstSCI CCNGG 1 cut(s) 800
BstSFI CTRYAG 2 cut(s) 447, 585
BstSLI GKGCMC 3 cut(s) 148, 641, 662
BstV1I GCAGC 4 cut(s) 423, 433, 511, 590
BstX2I RGATCY 2 cut(s) 155, 257
BstYI RGATCY 2 cut(s) 155, 257
BsuRI GGCC 1 cut(s) 401
BtsCI GGATG 1 cut(s) 697
BtsIMutI CAGTG 1 cut(s) 639
BveI ACCTGC 2 cut(s) 70, 440
Cac8I GCNNGC 2 cut(s) 601, 608
CciI TCATGA 1 cut(s) 198
Cfr13I GGNCC 3 cut(s) 371, 614, 804
Csp6I GTAC 3 cut(s) 100, 366, 721
CviAII CATG 3 cut(s) 199, 760, 769
CviJI RGCY 9 cut(s) 328, 381, 392, 401, 431, 436, 446, 628, 791
CviKI_1 RGCY 9 cut(s) 328, 381, 392, 401, 431, 436, 446, 628, 791
CviQI GTAC 3 cut(s) 100, 366, 721
DdeI CTNAG 7 cut(s) 303, 393, 427, 474, 482, 534, 776
DpnI GATC 3 cut(s) 157, 259, 336
DpnII GATC 3 cut(s) 155, 257, 334
EaeI YGGCCR 1 cut(s) 399
Eco31I GGTCTC 2 cut(s) 458, 663
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 3 cut(s) 371, 614, 804
EcoO109I RGGNCCY 2 cut(s) 614, 804
EcoRII CCWGG 1 cut(s) 800
EcoRV GATATC 1 cut(s) 514
FaeI CATG 3 cut(s) 202, 763, 772
FatI CATG 3 cut(s) 198, 759, 768
FauI CCCGC 1 cut(s) 599
FauNDI CATATG 1 cut(s) 574
Fnu4HI GCNGC 5 cut(s) 211, 437, 447, 525, 604
FokI GGATG 1 cut(s) 684
Fsp4HI GCNGC 5 cut(s) 211, 437, 447, 525, 604
FspBI CTAG 3 cut(s) 255, 546, 808
GluI GCNGC 5 cut(s) 211, 437, 447, 525, 604
GsaI CCCAGC 1 cut(s) 632
GsuI CTGGAG 1 cut(s) 784
HaeIII GGCC 1 cut(s) 401
HapII CCGG 1 cut(s) 744
Hin1II CATG 3 cut(s) 202, 763, 772
HincII GTYRAC 2 cut(s) 226, 562
HindII GTYRAC 2 cut(s) 226, 562
HinfI GANTC 3 cut(s) 489, 689, 797
HpaI GTTAAC 1 cut(s) 562
HpaII CCGG 1 cut(s) 744
HphI GGTGA 1 cut(s) 370
Hpy166II GTNNAC 6 cut(s) 85, 181, 226, 488, 562, 639
Hpy188I TCNGA 8 cut(s) 95, 155, 160, 304, 311, 477, 706, 732
Hpy188III TCNNGA 3 cut(s) 122, 199, 278
Hpy8I GTNNAC 6 cut(s) 85, 181, 226, 488, 562, 639
HpyAV CCTTC 2 cut(s) 284, 326
HpyCH4III ACNGT 2 cut(s) 205, 419
HpyCH4IV ACGT 2 cut(s) 38, 285
HpyCH4V TGCA 4 cut(s) 79, 439, 449, 639
HpyF10VI GCNNNNNNNGC 2 cut(s) 398, 596
HpyF3I CTNAG 7 cut(s) 303, 393, 427, 474, 482, 534, 776
HpySE526I ACGT 2 cut(s) 38, 285
Hsp92II CATG 3 cut(s) 202, 763, 772
KspAI GTTAAC 1 cut(s) 562
Kzo9I GATC 3 cut(s) 155, 257, 334
LmnI GCTCC 2 cut(s) 169, 185
Lsp1109I GCAGC 4 cut(s) 423, 433, 511, 590
LweI GCATC 1 cut(s) 468
MaeI CTAG 3 cut(s) 255, 546, 808
MaeII ACGT 2 cut(s) 38, 285
MaeIII GTNAC 2 cut(s) 358, 548
MalI GATC 3 cut(s) 157, 259, 336
MboI GATC 3 cut(s) 155, 257, 334
MboII GAAGA 1 cut(s) 508
MflI RGATCY 2 cut(s) 155, 257
MhlI GDGCHC 4 cut(s) 148, 190, 641, 662
MlsI TGGCCA 1 cut(s) 401
MluCI AATT 6 cut(s) 60, 72, 107, 229, 594, 652
MluNI TGGCCA 1 cut(s) 401
MlyI GAGTC 2 cut(s) 483, 791
MnlI CCTC 5 cut(s) 305, 318, 335, 576, 785
Mox20I TGGCCA 1 cut(s) 401
MscI TGGCCA 1 cut(s) 401
MseI TTAA 3 cut(s) 66, 459, 561
Msp20I TGGCCA 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 1 cut(s) 744
MspR9I CCNGG 1 cut(s) 802
MvaI CCWGG 1 cut(s) 802
MwoI GCNNNNNNNGC 2 cut(s) 398, 596
NdeI CATATG 1 cut(s) 574
NdeII GATC 3 cut(s) 155, 257, 334
NlaIII CATG 3 cut(s) 202, 763, 772
NlaIV GGNNCC 2 cut(s) 243, 508
NmeAIII GCCGAG 1 cut(s) 457
NmuCI GTSAC 2 cut(s) 358, 548
PagI TCATGA 1 cut(s) 198
PfeI GAWTC 1 cut(s) 689
PflFI GACNNNGTC 1 cut(s) 221
PfoI TCCNGGA 1 cut(s) 800
PkrI GCNGC 5 cut(s) 212, 438, 448, 526, 605
PleI GAGTC 2 cut(s) 483, 791
PpsI GAGTC 2 cut(s) 483, 791
PpuMI RGGWCCY 2 cut(s) 614, 804
Psp1406I AACGTT 1 cut(s) 38
Psp5II RGGWCCY 2 cut(s) 614, 804
Psp6I CCWGG 1 cut(s) 800
PspFI CCCAGC 1 cut(s) 628
PspGI CCWGG 1 cut(s) 800
PspN4I GGNNCC 2 cut(s) 243, 508
PspPI GGNCC 3 cut(s) 371, 614, 804
PspPPI RGGWCCY 2 cut(s) 614, 804
PstI CTGCAG 1 cut(s) 451
PsuI RGATCY 2 cut(s) 155, 257
PsyI GACNNNGTC 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 3 cut(s) 101, 367, 722
RsaNI GTAC 3 cut(s) 100, 366, 721
SaqAI TTAA 3 cut(s) 66, 459, 561
SatI GCNGC 5 cut(s) 211, 437, 447, 525, 604
Sau3AI GATC 3 cut(s) 155, 257, 334
Sau96I GGNCC 3 cut(s) 371, 614, 804
SchI GAGTC 2 cut(s) 483, 791
ScrFI CCNGG 1 cut(s) 802
SduI GDGCHC 4 cut(s) 148, 190, 641, 662
SfaNI GCATC 1 cut(s) 468
SfcI CTRYAG 2 cut(s) 447, 585
SinI GGWCC 3 cut(s) 371, 614, 804
SmlI CTYRAG 1 cut(s) 567
SmoI CTYRAG 1 cut(s) 567
Sse9I AATT 6 cut(s) 60, 72, 107, 229, 594, 652
SsiI CCGC 2 cut(s) 210, 606
SspI AATATT 2 cut(s) 269, 736
SspMI CTAG 3 cut(s) 255, 546, 808
StyD4I CCNGG 1 cut(s) 800
TaaI ACNGT 2 cut(s) 205, 419
TaiI ACGT 2 cut(s) 41, 288
TasI AATT 6 cut(s) 60, 72, 107, 229, 594, 652
TatI WGTACW 1 cut(s) 99
TauI GCSGC 1 cut(s) 213
TfiI GAWTC 1 cut(s) 689
Tru1I TTAA 3 cut(s) 66, 459, 561
Tru9I TTAA 3 cut(s) 66, 459, 561
TscAI CASTG 1 cut(s) 646
TseFI GTSAC 2 cut(s) 358, 548
TseI GCWGC 4 cut(s) 436, 446, 524, 603
Tsp45I GTSAC 2 cut(s) 358, 548
TspDTI ATGAA 5 cut(s) 508, 681, 686, 776, 785
TspGWI ACGGA 2 cut(s) 182, 198
TspRI CASTG 1 cut(s) 646
Tth111I GACNNNGTC 1 cut(s) 221
VneI GTGCAC 1 cut(s) 637
VpaK11BI GGWCC 3 cut(s) 371, 614, 804
XapI RAATTY 1 cut(s) 60
XcmI CCANNNNNNNNNTGG 1 cut(s) 790
XspI CTAG 3 cut(s) 255, 546, 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.