FvH4_6g01380

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007620
Physical Location & Seq
Reverse (-)
767004 .. 769191
2188 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01380.t1

Sequence Viewer

Length: 1098 bp
ATGGCTAGCTCACTGGTGCTGGTTTTCAGTCTAGCCCTCACTGCATTGGGGAGCTTCATGATCCCCCAGCAGGTCGAGGCTCGTGCCTTCTTCGTGTTCGGAGACTCATTGGTCGACAATGGCAACAACAATTACTTAGCAACTCAGGCTCGTGCCGATGCTCCTCCTTATGGCATTGACTATCCAACTGGTCGAGCAACGGGTCGTTTCTCCAATGGCTACAACATCCCTGATTTCATCAGTCAAGCACTTGGAGCAGAAGCCACATTGCCTTACTTGAGTCCGGAGCTCAATGGAGAAAAGCTACTTGTTGGTGCCAACTTTGCTTCTGCTGGCATCGGAATCCTCAATGACACCGGAGTTCAGTTTATAAACATAATCAGAATGTTCAGGCAATATGAGTACTTTGAACAATACCAGCAAAGGTTGAGTGCCCTTATCGGACCTGAACAGGCGCAGCAACTTGTGAACGATGCACTTATTCTCTTGACTGTTGGCGGCAATGATTTCGTCAACAACTACTACTTGGTTCCTTTCTCTGCTAGATCTCGTCAATATCCTCTTCCAGAATATGTCGAATACCTCATTTCCGAGTTCAGAAAGCTTCTTCTGAGGCTATACGATCTTGGAGGGCGAAGGGTTTTGGTGACAGGTACAGGACCATTAGGGTGTGTTCCAGCCGAGTTGGCCATGAGAAGTACTAATGGTCAATGCGCACCCGAGCTGCAAAGAGCTGCAAGTCTCTTTAACCCACAACTTACTCAGATGATACGTGAAGTCAACAGTGAATTAGGTGCCGATATCTTCATTGCTGCCAACACTCAGCTCAGTCACTACGACTTCGTGTCTAACCCTCAAGCATATGGATTTACGACATCGAAAATTGCCTGCTGCGGGCAAGGACCTTACAATGGGCTGGGATTGTGCACTGCATTGTCCAACGTGTGCCCGGACAGAGACCTATATGCTTTCTGGGATGCATTTCATCCATCGGAAAGGGCGAACCGGTACGTTGTTCAGGCAATTCTGAACGGTGGAACCGAGTACATGAGCCCCATGAACCTCAGCACCATTTTGGCTTTGGATTCCAGGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

366

Amino Acids

40.07

Weight (kDa)

4.89

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 342 4.7e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AasI GACNNNNNNGTC 1 cut(s) 110
Acc16I TGCGCA 1 cut(s) 715
Acc36I ACCTGC 1 cut(s) 61
AccB1I GGYRCC 2 cut(s) 314, 794
AccI GTMKAC 1 cut(s) 114
AccIII TCCGGA 1 cut(s) 283
AciI CCGC 2 cut(s) 498, 894
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 1 cut(s) 687
AfaI GTAC 5 cut(s) 404, 655, 700, 1010, 1046
AfiI CCNNNNNNNGG 4 cut(s) 70, 170, 894, 911
AflIII ACRYGT 1 cut(s) 942
AgeI ACCGGT 1 cut(s) 1005
AgsI TTSAA 1 cut(s) 410
AhdI GACNNNNNGTC 1 cut(s) 844
AjnI CCWGG 1 cut(s) 1088
AluBI AGCT 8 cut(s) 9, 54, 289, 304, 604, 724, 734, 826
AluI AGCT 8 cut(s) 9, 54, 289, 304, 604, 724, 734, 826
Alw21I GWGCWC 2 cut(s) 291, 929
Alw26I GTCTC 3 cut(s) 96, 746, 951
Alw44I GTGCAC 1 cut(s) 925
AlwI GGATC 1 cut(s) 55
Ama87I CYCGRG 1 cut(s) 719
Aor13HI TCCGGA 1 cut(s) 283
AoxI GGCC 1 cut(s) 687
ApaLI GTGCAC 1 cut(s) 925
ApeKI GCWGC 5 cut(s) 457, 724, 734, 812, 891
AsiGI ACCGGT 1 cut(s) 1005
AspLEI GCGC 2 cut(s) 457, 716
AspS9I GGNCC 4 cut(s) 443, 659, 902, 1092
AsuC2I CCSGG 1 cut(s) 950
AsuHPI GGTGA 1 cut(s) 658
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 1 cut(s) 719
AvaII GGWCC 4 cut(s) 443, 659, 902, 1092
BaeGI GKGCMC 3 cut(s) 436, 929, 950
BalI TGGCCA 1 cut(s) 689
BanI GGYRCC 2 cut(s) 314, 794
BanII GRGCYC 2 cut(s) 291, 1055
BauI CACGAG 2 cut(s) 81, 150
Bbv12I GWGCWC 2 cut(s) 291, 929
BbvCI CCTCAGC 1 cut(s) 1064
BbvI GCAGC 5 cut(s) 469, 711, 721, 799, 878
BccI CCATC 1 cut(s) 997
BcgI CGANNNNNNTGC 4 cut(s) 65, 99, 490, 524
BciT130I CCWGG 1 cut(s) 1090
BcnI CCSGG 1 cut(s) 950
BcoDI GTCTC 3 cut(s) 96, 746, 951
BfaI CTAG 3 cut(s) 6, 32, 543
BfuAI ACCTGC 1 cut(s) 61
BglI GCCNNNNNGGC 1 cut(s) 686
BglII AGATCT 1 cut(s) 545
BisI GCNGC 6 cut(s) 458, 499, 725, 735, 813, 892
BlsI GCNGC 6 cut(s) 459, 500, 726, 736, 814, 893
BmcAI AGTACT 2 cut(s) 404, 700
Bme1390I CCNGG 2 cut(s) 950, 1090
Bme18I GGWCC 4 cut(s) 443, 659, 902, 1092
BmeRI GACNNNNNGTC 1 cut(s) 844
BmeT110I CYCGRG 1 cut(s) 719
BmgT120I GGNCC 4 cut(s) 443, 659, 902, 1092
BmiI GGNNCC 4 cut(s) 316, 531, 796, 1039
BmrFI CCNGG 2 cut(s) 950, 1090
BmsI GCATC 4 cut(s) 148, 345, 463, 967
BmtI GCTAGC 1 cut(s) 9
Bpu10I CCTNAGC 1 cut(s) 1064
BpuEI CTTGAG 2 cut(s) 298, 840
BpuMI CCSGG 1 cut(s) 950
BsaAI YACGTR 1 cut(s) 773
BsaI GGTCTC 1 cut(s) 951
BsaWI WCCGGW 3 cut(s) 283, 356, 1005
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 170, 894, 911
Bse118I RCCGGY 1 cut(s) 1005
Bse1I ACTGG 2 cut(s) 18, 193
Bse3DI GCAATG 3 cut(s) 266, 508, 807
BseAI TCCGGA 1 cut(s) 283
BseBI CCWGG 1 cut(s) 1090
BseGI GGATG 3 cut(s) 225, 982, 985
BseLI CCNNNNNNNGG 4 cut(s) 70, 170, 894, 911
BseMI GCAATG 3 cut(s) 266, 508, 807
BseMII CTCAG 6 cut(s) 158, 602, 776, 836, 841, 1078
BseNI ACTGG 2 cut(s) 18, 193
BseRI GAGGAG 1 cut(s) 153
BseSI GKGCMC 3 cut(s) 436, 929, 950
BseXI GCAGC 5 cut(s) 469, 711, 721, 799, 878
BseYI CCCAGC 2 cut(s) 66, 916
BshFI GGCC 1 cut(s) 689
BshNI GGYRCC 2 cut(s) 314, 794
BshTI ACCGGT 1 cut(s) 1005
BsiHKAI GWGCWC 2 cut(s) 291, 929
BsiHKCI CYCGRG 1 cut(s) 719
BsiSI CCGG 4 cut(s) 284, 357, 950, 1006
BslI CCNNNNNNNGG 4 cut(s) 70, 170, 894, 911
BsmAI GTCTC 3 cut(s) 96, 746, 951
BsnI GGCC 1 cut(s) 689
Bso31I GGTCTC 1 cut(s) 951
BsoBI CYCGRG 1 cut(s) 719
Bsp1286I GDGCHC 5 cut(s) 291, 436, 929, 950, 1055
Bsp13I TCCGGA 1 cut(s) 283
Bsp143I GATC 3 cut(s) 60, 545, 622
BspACI CCGC 2 cut(s) 498, 894
BspANI GGCC 1 cut(s) 689
BspCNI CTCAG 6 cut(s) 157, 603, 775, 835, 840, 1077
BspEI TCCGGA 1 cut(s) 283
BspHI TCATGA 1 cut(s) 57
BspLI GGNNCC 4 cut(s) 316, 531, 796, 1039
BspMI ACCTGC 1 cut(s) 61
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 55
BspT107I GGYRCC 2 cut(s) 314, 794
BspTNI GGTCTC 1 cut(s) 951
BsrDI GCAATG 3 cut(s) 266, 508, 807
BsrFI RCCGGY 1 cut(s) 1005
BsrI ACTGG 2 cut(s) 18, 193
BssAI RCCGGY 1 cut(s) 1005
BssMI GATC 3 cut(s) 60, 545, 622
BssSI CACGAG 2 cut(s) 81, 150
Bst2BI CACGAG 2 cut(s) 81, 150
Bst2UI CCWGG 1 cut(s) 1090
Bst4CI ACNGT 3 cut(s) 493, 785, 1034
Bst6I CTCTTC 1 cut(s) 567
BstBAI YACGTR 1 cut(s) 773
BstC8I GCNNGC 4 cut(s) 7, 334, 889, 896
BstDEI CTNAG 7 cut(s) 136, 144, 611, 762, 822, 827, 1064
BstF5I GGATG 3 cut(s) 225, 982, 985
BstHHI GCGC 2 cut(s) 457, 716
BstKTI GATC 3 cut(s) 63, 548, 625
BstMAI GTCTC 3 cut(s) 96, 746, 951
BstMBI GATC 3 cut(s) 60, 545, 622
BstMWI GCNNNNNNNGC 5 cut(s) 41, 146, 254, 323, 686
BstNI CCWGG 1 cut(s) 1090
BstSCI CCNGG 2 cut(s) 948, 1088
BstSLI GKGCMC 3 cut(s) 436, 929, 950
BstV1I GCAGC 5 cut(s) 469, 711, 721, 799, 878
BstX2I RGATCY 1 cut(s) 545
BstYI RGATCY 1 cut(s) 545
BsuRI GGCC 1 cut(s) 689
BtsCI GGATG 3 cut(s) 225, 982, 985
BtsI GCAGTG 2 cut(s) 39, 927
BtsIMutI CAGTG 4 cut(s) 11, 39, 790, 927
BveI ACCTGC 1 cut(s) 61
Cac8I GCNNGC 4 cut(s) 7, 334, 889, 896
CciI TCATGA 1 cut(s) 57
CfoI GCGC 2 cut(s) 457, 716
Cfr10I RCCGGY 1 cut(s) 1005
Cfr13I GGNCC 4 cut(s) 443, 659, 902, 1092
Csp6I GTAC 5 cut(s) 403, 654, 699, 1009, 1045
CspAI ACCGGT 1 cut(s) 1005
CviAII CATG 4 cut(s) 58, 691, 1048, 1057
CviQI GTAC 5 cut(s) 403, 654, 699, 1009, 1045
DdeI CTNAG 7 cut(s) 136, 144, 611, 762, 822, 827, 1064
DpnI GATC 3 cut(s) 62, 547, 624
DpnII GATC 3 cut(s) 60, 545, 622
DrdI GACNNNNNNGTC 1 cut(s) 110
DriI GACNNNNNGTC 1 cut(s) 844
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 1 cut(s) 687
Eam1104I CTCTTC 1 cut(s) 567
Eam1105I GACNNNNNGTC 1 cut(s) 844
EarI CTCTTC 1 cut(s) 567
Ecl136II GAGCTC 1 cut(s) 289
Eco24I GRGCYC 2 cut(s) 291, 1055
Eco31I GGTCTC 1 cut(s) 951
Eco32I GATATC 1 cut(s) 802
Eco47I GGWCC 4 cut(s) 443, 659, 902, 1092
Eco53kI GAGCTC 1 cut(s) 289
Eco88I CYCGRG 1 cut(s) 719
EcoICRI GAGCTC 1 cut(s) 289
EcoO109I RGGNCCY 2 cut(s) 902, 1092
EcoRII CCWGG 1 cut(s) 1088
EcoRV GATATC 1 cut(s) 802
EcoT22I ATGCAT 1 cut(s) 982
EcoT38I GRGCYC 2 cut(s) 291, 1055
FaeI CATG 4 cut(s) 61, 694, 1051, 1060
FatI CATG 4 cut(s) 57, 690, 1047, 1056
FauI CCCGC 1 cut(s) 887
FauNDI CATATG 1 cut(s) 862
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 6 cut(s) 458, 499, 725, 735, 813, 892
FokI GGATG 3 cut(s) 212, 972, 989
FriOI GRGCYC 2 cut(s) 291, 1055
Fsp4HI GCNGC 6 cut(s) 458, 499, 725, 735, 813, 892
FspAI RTGCGCAY 1 cut(s) 715
FspBI CTAG 3 cut(s) 6, 32, 543
FspI TGCGCA 1 cut(s) 715
GlaI GCGC 2 cut(s) 456, 715
GluI GCNGC 6 cut(s) 458, 499, 725, 735, 813, 892
GsaI CCCAGC 2 cut(s) 70, 920
HaeIII GGCC 1 cut(s) 689
HapII CCGG 4 cut(s) 284, 357, 950, 1006
HhaI GCGC 2 cut(s) 457, 716
Hin1II CATG 4 cut(s) 61, 694, 1051, 1060
Hin6I GCGC 2 cut(s) 455, 714
HinP1I GCGC 2 cut(s) 455, 714
HincII GTYRAC 3 cut(s) 115, 514, 781
HindII GTYRAC 3 cut(s) 115, 514, 781
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 4 cut(s) 104, 280, 342, 1085
HpaII CCGG 4 cut(s) 284, 357, 950, 1006
HphI GGTGA 1 cut(s) 658
Hpy166II GTNNAC 5 cut(s) 115, 469, 514, 781, 927
Hpy188III TCNNGA 4 cut(s) 58, 284, 487, 566
Hpy8I GTNNAC 5 cut(s) 115, 469, 514, 781, 927
HpyAV CCTTC 2 cut(s) 97, 630
HpyCH4III ACNGT 3 cut(s) 493, 785, 1034
HpyCH4IV ACGT 3 cut(s) 772, 942, 1011
HpyCH4V TGCA 7 cut(s) 44, 476, 727, 737, 927, 932, 980
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 146, 254, 323, 686
HpyF3I CTNAG 7 cut(s) 136, 144, 611, 762, 822, 827, 1064
HpySE526I ACGT 3 cut(s) 772, 942, 1011
Hsp92II CATG 4 cut(s) 61, 694, 1051, 1060
HspAI GCGC 2 cut(s) 455, 714
Kpn2I TCCGGA 1 cut(s) 283
Kzo9I GATC 3 cut(s) 60, 545, 622
LmnI GCTCC 4 cut(s) 51, 166, 254, 286
Lsp1109I GCAGC 5 cut(s) 469, 711, 721, 799, 878
LweI GCATC 4 cut(s) 148, 345, 463, 967
MaeI CTAG 3 cut(s) 6, 32, 543
MaeII ACGT 3 cut(s) 772, 942, 1011
MaeIII GTNAC 2 cut(s) 646, 830
MalI GATC 3 cut(s) 62, 547, 624
MboI GATC 3 cut(s) 60, 545, 622
MboII GAAGA 4 cut(s) 82, 554, 599, 796
MflI RGATCY 1 cut(s) 545
MhlI GDGCHC 5 cut(s) 291, 436, 929, 950, 1055
MlsI TGGCCA 1 cut(s) 689
MluCI AATT 4 cut(s) 130, 788, 882, 1023
MluNI TGGCCA 1 cut(s) 689
MlyI GAGTC 2 cut(s) 98, 289
MmeI TCCRAC 2 cut(s) 209, 963
Mox20I TGGCCA 1 cut(s) 689
Mph1103I ATGCAT 1 cut(s) 982
MroI TCCGGA 1 cut(s) 283
MscI TGGCCA 1 cut(s) 689
MseI TTAA 1 cut(s) 747
MslI CAYNNNNRTG 1 cut(s) 667
Msp20I TGGCCA 1 cut(s) 689
MspI CCGG 4 cut(s) 284, 357, 950, 1006
MspR9I CCNGG 2 cut(s) 950, 1090
MvaI CCWGG 1 cut(s) 1090
MwoI GCNNNNNNNGC 5 cut(s) 41, 146, 254, 323, 686
NciI CCSGG 1 cut(s) 950
NdeI CATATG 1 cut(s) 862
NdeII GATC 3 cut(s) 60, 545, 622
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 4 cut(s) 61, 694, 1051, 1060
NlaIV GGNNCC 4 cut(s) 316, 531, 796, 1039
NmeAIII GCCGAG 1 cut(s) 706
NmuCI GTSAC 2 cut(s) 646, 830
NsbI TGCGCA 1 cut(s) 715
NsiI ATGCAT 1 cut(s) 982
PagI TCATGA 1 cut(s) 57
PcsI WCGNNNNNNNCGW 2 cut(s) 998, 1038
PfeI GAWTC 2 cut(s) 342, 1085
PfoI TCCNGGA 1 cut(s) 1088
PinAI ACCGGT 1 cut(s) 1005
PkrI GCNGC 6 cut(s) 459, 500, 726, 736, 814, 893
PleI GAGTC 2 cut(s) 98, 288
PpsI GAGTC 2 cut(s) 98, 288
Ppu21I YACGTR 1 cut(s) 773
PpuMI RGGWCCY 2 cut(s) 902, 1092
PsiI TTATAA 1 cut(s) 371
Psp124BI GAGCTC 1 cut(s) 291
Psp5II RGGWCCY 2 cut(s) 902, 1092
Psp6I CCWGG 1 cut(s) 1088
PspFI CCCAGC 2 cut(s) 66, 916
PspGI CCWGG 1 cut(s) 1088
PspN4I GGNNCC 4 cut(s) 316, 531, 796, 1039
PspPI GGNCC 4 cut(s) 443, 659, 902, 1092
PspPPI RGGWCCY 2 cut(s) 902, 1092
PsuI RGATCY 1 cut(s) 545
RsaI GTAC 5 cut(s) 404, 655, 700, 1010, 1046
RsaNI GTAC 5 cut(s) 403, 654, 699, 1009, 1045
RseI CAYNNNNRTG 1 cut(s) 667
SacI GAGCTC 1 cut(s) 291
SalI GTCGAC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 747
SatI GCNGC 6 cut(s) 458, 499, 725, 735, 813, 892
Sau3AI GATC 3 cut(s) 60, 545, 622
Sau96I GGNCC 4 cut(s) 443, 659, 902, 1092
ScaI AGTACT 2 cut(s) 404, 700
SchI GAGTC 2 cut(s) 98, 289
ScrFI CCNGG 2 cut(s) 950, 1090
SduI GDGCHC 5 cut(s) 291, 436, 929, 950, 1055
SfaNI GCATC 4 cut(s) 148, 345, 463, 967
SinI GGWCC 4 cut(s) 443, 659, 902, 1092
SmiMI CAYNNNNRTG 1 cut(s) 667
SmlI CTYRAG 2 cut(s) 277, 855
SmoI CTYRAG 2 cut(s) 277, 855
Sse9I AATT 4 cut(s) 130, 788, 882, 1023
SsiI CCGC 2 cut(s) 498, 894
SspMI CTAG 3 cut(s) 6, 32, 543
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 2 cut(s) 948, 1088
TaaI ACNGT 3 cut(s) 493, 785, 1034
TaiI ACGT 3 cut(s) 775, 945, 1014
TaqI TCGA 5 cut(s) 75, 114, 193, 576, 878
TasI AATT 4 cut(s) 130, 788, 882, 1023
TatI WGTACW 3 cut(s) 402, 698, 1044
TauI GCSGC 1 cut(s) 501
TfiI GAWTC 2 cut(s) 342, 1085
Tru1I TTAA 1 cut(s) 747
Tru9I TTAA 1 cut(s) 747
TscAI CASTG 4 cut(s) 18, 46, 790, 934
TseFI GTSAC 2 cut(s) 646, 830
TseI GCWGC 5 cut(s) 457, 724, 734, 812, 891
Tsp45I GTSAC 2 cut(s) 646, 830
TspDTI ATGAA 5 cut(s) 46, 226, 796, 974, 1073
TspRI CASTG 4 cut(s) 18, 46, 790, 934
VneI GTGCAC 1 cut(s) 925
VpaK11BI GGWCC 4 cut(s) 443, 659, 902, 1092
XcmI CCANNNNNNNNNTGG 1 cut(s) 1078
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 3 cut(s) 6, 32, 543
ZrmI AGTACT 2 cut(s) 404, 700
Zsp2I ATGCAT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.