AT5G18870

Inosine-uridine preferring nucleoside hydrolase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001810
Physical Location & Seq
Forward (+)
6298386 .. 6300289
1904 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G18870.1

Sequence Viewer

Length: 777 bp
ATGTTGAGTCCACGAAGGTTCATTCTAGTCGTCACCATTCTTGGACTCTTAGGTCATAACCTCACACATGTTCTGAATAGCCCACACCGTATTCTCTTGGACACAGATGTCGACACAGACGATTTCATCGCTCTCTTGTACCTCTTGAAGCTCAACAAAACAGAATTCGATCTAGTCGGGATCACATTAAGTGCCAATTCTTGGACAAACGCAGGACATGGAGTGAATCATATATACGACATTCTTTACATGATGGGTCGTGACGATATCACCGTTGGTGTTGGAGGAGAAGGTGGGATTCTCGAAGATGGTACTATTCTTCCTGACGTCGGTGATTATCTTCCGATCATCGAACAGGGGATGACAACAGCAGGAGGATGCAGATACAGACAATCCATTCCAAAAGGTCGTATACAAAAGATTGACTCCAATTATGGATTTCGTAAACATTTTCTTCCTCAGGGGAACCGAAGATACACTCCTCTCGAGCAACCAACGGCACAAAAAGTAATCGTCGACAAAGTTTCTGAAGGTCCAATCAGCATCTTCGTGATCGGTTCTCACACCAATCTAGCTCTCTTCATGATGTCAAATCCTCACCTCAAACATAACATCCAACACATTTACGTTATGGGTGGTAGTGTACGCTGTCAAAACCCTAACGGATTCTGCGGAAACTTGTTCACAGATTATACAAGTAATCCTTACGCTGAATTCAACATCTTCACCGATCCTTTTGCTGCTTACCAGGTGTTCCGATTACTATGGTTCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
IU_nuc_hydro

Protein Analysis

258

Amino Acids

28.92

Weight (kDa)

6.34

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IU_nuc_hydro PF01156 31 - 253 1.2e-31 Inosine-uridine preferring nucleoside hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 51, 107
AatII GACGTC 1 cut(s) 330
AccB7I CCANNNNNTGG 1 cut(s) 201
AccI GTMKAC 3 cut(s) 111, 412, 516
AciI CCGC 1 cut(s) 672
AclWI GGATC 2 cut(s) 188, 725
AcsI RAATTY 2 cut(s) 164, 713
AcuI CTGAAG 1 cut(s) 549
AcyI GRCGYC 1 cut(s) 327
AfaI GTAC 3 cut(s) 140, 313, 645
AfiI CCNNNNNNNGG 2 cut(s) 201, 329
AflIII ACRYGT 1 cut(s) 67
AgsI TTSAA 2 cut(s) 148, 718
AjnI CCWGG 1 cut(s) 747
AluBI AGCT 2 cut(s) 151, 575
AluI AGCT 2 cut(s) 151, 575
AlwI GGATC 2 cut(s) 188, 725
Ama87I CYCGRG 1 cut(s) 485
ApeKI GCWGC 1 cut(s) 740
ApoI RAATTY 2 cut(s) 164, 713
AspS9I GGNCC 1 cut(s) 533
AsuHPI GGTGA 5 cut(s) 25, 262, 344, 590, 718
AvaI CYCGRG 1 cut(s) 485
AvaII GGWCC 1 cut(s) 533
AxyI CCTNAGG 1 cut(s) 459
BbvI GCAGC 1 cut(s) 727
BccI CCATC 2 cut(s) 247, 302
BceAI ACGGC 1 cut(s) 513
BcgI CGANNNNNNTGC 2 cut(s) 719, 753
BciT130I CCWGG 1 cut(s) 749
BfaI CTAG 3 cut(s) 26, 173, 572
BisI GCNGC 1 cut(s) 741
BlsI GCNGC 1 cut(s) 742
Bme1390I CCNGG 1 cut(s) 749
Bme18I GGWCC 1 cut(s) 533
BmeT110I CYCGRG 1 cut(s) 485
BmgT120I GGNCC 1 cut(s) 533
BmiI GGNNCC 2 cut(s) 467, 770
BmrFI CCNGG 1 cut(s) 749
BmsI GCATC 2 cut(s) 368, 552
BsaHI GRCGYC 1 cut(s) 327
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 329
Bse21I CCTNAGG 1 cut(s) 459
BseBI CCWGG 1 cut(s) 749
BseGI GGATG 3 cut(s) 366, 383, 612
BseLI CCNNNNNNNGG 2 cut(s) 201, 329
BseMII CTCAG 1 cut(s) 473
BseRI GAGGAG 2 cut(s) 300, 471
BseXI GCAGC 1 cut(s) 727
BsiHKCI CYCGRG 1 cut(s) 485
BslI CCNNNNNNNGG 2 cut(s) 201, 329
BsoBI CYCGRG 1 cut(s) 485
Bsp143I GATC 5 cut(s) 169, 180, 345, 552, 730
BspACI CCGC 1 cut(s) 672
BspCNI CTCAG 1 cut(s) 472
BspHI TCATGA 1 cut(s) 582
BspLI GGNNCC 2 cut(s) 467, 770
BspPI GGATC 2 cut(s) 188, 725
BssMI GATC 5 cut(s) 169, 180, 345, 552, 730
BssNAI GTATAC 1 cut(s) 413
BssNI GRCGYC 1 cut(s) 327
Bst1107I GTATAC 1 cut(s) 413
Bst2UI CCWGG 1 cut(s) 749
Bst4CI ACNGT 2 cut(s) 89, 274
Bst6I CTCTTC 1 cut(s) 584
BstACI GRCGYC 1 cut(s) 327
BstDEI CTNAG 2 cut(s) 49, 459
BstF5I GGATG 3 cut(s) 366, 383, 612
BstKTI GATC 5 cut(s) 172, 183, 348, 555, 733
BstMBI GATC 5 cut(s) 169, 180, 345, 552, 730
BstNI CCWGG 1 cut(s) 749
BstNSI RCATGY 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 747
BstV1I GCAGC 1 cut(s) 727
BstZ17I GTATAC 1 cut(s) 413
Bsu36I CCTNAGG 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 112
BtsCI GGATG 3 cut(s) 366, 383, 612
CciI TCATGA 1 cut(s) 582
Cfr13I GGNCC 1 cut(s) 533
CsiI ACCWGGT 1 cut(s) 747
Csp6I GTAC 3 cut(s) 139, 312, 644
CviAII CATG 4 cut(s) 68, 218, 250, 583
CviJI RGCY 3 cut(s) 81, 151, 575
CviKI_1 RGCY 3 cut(s) 81, 151, 575
CviQI GTAC 3 cut(s) 139, 312, 644
DdeI CTNAG 2 cut(s) 49, 459
DpnI GATC 5 cut(s) 171, 182, 347, 554, 732
DpnII GATC 5 cut(s) 169, 180, 345, 552, 730
DrdI GACNNNNNNGTC 2 cut(s) 51, 107
DseDI GACNNNNNNGTC 2 cut(s) 51, 107
Eam1104I CTCTTC 1 cut(s) 584
EarI CTCTTC 1 cut(s) 584
Eco32I GATATC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 533
Eco57I CTGAAG 1 cut(s) 549
Eco81I CCTNAGG 1 cut(s) 459
Eco88I CYCGRG 1 cut(s) 485
EcoRI GAATTC 2 cut(s) 164, 713
EcoRII CCWGG 1 cut(s) 747
EcoRV GATATC 1 cut(s) 268
FaeI CATG 4 cut(s) 71, 221, 253, 586
FalI AAGNNNNNCTT 2 cut(s) 688, 720
FatI CATG 4 cut(s) 67, 217, 249, 582
FblI GTMKAC 3 cut(s) 111, 412, 516
Fnu4HI GCNGC 1 cut(s) 741
FokI GGATG 3 cut(s) 373, 390, 599
Fsp4HI GCNGC 1 cut(s) 741
FspBI CTAG 3 cut(s) 26, 173, 572
GluI GCNGC 1 cut(s) 741
Hin1I GRCGYC 1 cut(s) 327
Hin1II CATG 4 cut(s) 71, 221, 253, 586
HincII GTYRAC 2 cut(s) 112, 517
HindII GTYRAC 2 cut(s) 112, 517
HinfI GANTC 6 cut(s) 7, 45, 226, 298, 425, 666
HphI GGTGA 5 cut(s) 25, 262, 344, 590, 718
Hpy166II GTNNAC 7 cut(s) 11, 112, 413, 446, 517, 644, 684
Hpy188I TCNGA 4 cut(s) 75, 345, 529, 758
Hpy188III TCNNGA 8 cut(s) 145, 178, 260, 302, 323, 485, 550, 583
Hpy8I GTNNAC 7 cut(s) 11, 112, 413, 446, 517, 644, 684
Hpy99I CGWCG 2 cut(s) 332, 518
HpyAV CCTTC 3 cut(s) 9, 284, 524
HpyCH4III ACNGT 2 cut(s) 89, 274
HpyCH4IV ACGT 2 cut(s) 327, 627
HpyCH4V TGCA 1 cut(s) 381
HpyF3I CTNAG 2 cut(s) 49, 459
HpySE526I ACGT 2 cut(s) 327, 627
Hsp92I GRCGYC 1 cut(s) 327
Hsp92II CATG 4 cut(s) 71, 221, 253, 586
Kzo9I GATC 5 cut(s) 169, 180, 345, 552, 730
LpnPI CCDG 7 cut(s) 198, 336, 341, 357, 446, 734, 761
Lsp1109I GCAGC 1 cut(s) 727
LweI GCATC 2 cut(s) 368, 552
MabI ACCWGGT 1 cut(s) 747
MaeI CTAG 3 cut(s) 26, 173, 572
MaeII ACGT 2 cut(s) 327, 627
MaeIII GTNAC 2 cut(s) 31, 260
MalI GATC 5 cut(s) 171, 182, 347, 554, 732
MboI GATC 5 cut(s) 169, 180, 345, 552, 730
MboII GAAGA 8 cut(s) 311, 317, 332, 446, 483, 538, 571, 715
MluCI AATT 4 cut(s) 164, 196, 430, 713
MlyI GAGTC 3 cut(s) 16, 39, 419
MmeI TCCRAC 2 cut(s) 262, 640
MnlI CCTC 8 cut(s) 71, 152, 278, 368, 468, 492, 606, 611
MseI TTAA 1 cut(s) 188
MslI CAYNNNNRTG 1 cut(s) 548
MspR9I CCNGG 1 cut(s) 749
MvaI CCWGG 1 cut(s) 749
NdeII GATC 5 cut(s) 169, 180, 345, 552, 730
NlaIII CATG 4 cut(s) 71, 221, 253, 586
NlaIV GGNNCC 2 cut(s) 467, 770
NmuCI GTSAC 2 cut(s) 31, 260
NspI RCATGY 1 cut(s) 71
PaeR7I CTCGAG 1 cut(s) 485
PagI TCATGA 1 cut(s) 582
PciI ACATGT 1 cut(s) 67
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 270
PfeI GAWTC 3 cut(s) 226, 298, 666
PflMI CCANNNNNTGG 1 cut(s) 201
PkrI GCNGC 1 cut(s) 742
PleI GAGTC 3 cut(s) 15, 39, 419
PpsI GAGTC 3 cut(s) 15, 39, 419
PscI ACATGT 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 747
PspGI CCWGG 1 cut(s) 747
PspN4I GGNNCC 2 cut(s) 467, 770
PspPI GGNCC 1 cut(s) 533
PsrI GAACNNNNNNTAC 2 cut(s) 458, 490
RsaI GTAC 3 cut(s) 140, 313, 645
RsaNI GTAC 3 cut(s) 139, 312, 644
RseI CAYNNNNRTG 1 cut(s) 548
SalI GTCGAC 2 cut(s) 110, 515
SaqAI TTAA 1 cut(s) 188
SatI GCNGC 1 cut(s) 741
Sau3AI GATC 5 cut(s) 169, 180, 345, 552, 730
Sau96I GGNCC 1 cut(s) 533
SchI GAGTC 3 cut(s) 16, 39, 419
ScrFI CCNGG 1 cut(s) 749
SexAI ACCWGGT 1 cut(s) 747
SfaNI GCATC 2 cut(s) 368, 552
Sfr274I CTCGAG 1 cut(s) 485
SinI GGWCC 1 cut(s) 533
SlaI CTCGAG 1 cut(s) 485
SmiMI CAYNNNNRTG 1 cut(s) 548
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 4 cut(s) 164, 196, 430, 713
SsiI CCGC 1 cut(s) 672
SspMI CTAG 3 cut(s) 26, 173, 572
StyD4I CCNGG 1 cut(s) 747
TaaI ACNGT 2 cut(s) 89, 274
TaiI ACGT 2 cut(s) 330, 630
TaqI TCGA 6 cut(s) 111, 168, 303, 351, 486, 516
TasI AATT 4 cut(s) 164, 196, 430, 713
TfiI GAWTC 3 cut(s) 226, 298, 666
Tru1I TTAA 1 cut(s) 188
Tru9I TTAA 1 cut(s) 188
TseFI GTSAC 2 cut(s) 31, 260
TseI GCWGC 1 cut(s) 740
Tsp45I GTSAC 2 cut(s) 31, 260
TspDTI ATGAA 3 cut(s) 10, 115, 571
TspGWI ACGGA 1 cut(s) 678
Van91I CCANNNNNTGG 1 cut(s) 201
VpaK11BI GGWCC 1 cut(s) 533
XapI RAATTY 2 cut(s) 164, 713
XceI RCATGY 1 cut(s) 71
XhoI CTCGAG 1 cut(s) 485
XmiI GTMKAC 3 cut(s) 111, 412, 516
XspI CTAG 3 cut(s) 26, 173, 572
ZraI GACGTC 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.