Rorug04G0163700

Inosine-uridine preferring nucleoside hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001810
Physical Location & Seq
Forward (+)
28517516 .. 28519969
2454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0163700.1

Sequence Viewer

Length: 387 bp
ATGGGTGATGCAGCAGAGATGCAATCTCCAAAGGAAGAGATGGTGGCGTGTGTGAGGGCACTGGAGGATGCTTTGCTTCCATGCTTGCCGGCCCGAGAGCTCCAAGCAATTGACCGTTCTCCCCACCCTTCACATCAGATTGATGTGGAGAGACATGCCAGAGACTTTATGGACGCTGCCAAAAAGCTTCAGCTGCATTTTCTTGGTCTGCAACGTGAGGACCAGCCAACAAATGCTGAACAACTTAGAAAGGAGATTGCTGTGATGGAAGAGGAGTTGAATATAAAGACTGAGATCATCAAGAAGCATGAAAGGCTAATCCAGGGGTGGAAAAAGGAGCTAAAAGATCAATTGAACAAGCACAATACCGAGCTTGAGAAGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
IU_nuc_hydro

Protein Analysis

128

Amino Acids

14.85

Weight (kDa)

5.7

Isoelectric Point (pI)

63.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med28 PF11594 17 - 114 4.6e-25 Mediator complex subunit 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 173
AgsI TTSAA 2 cut(s) 280, 355
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 5 cut(s) 100, 187, 193, 340, 373
AluI AGCT 5 cut(s) 100, 187, 193, 340, 373
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 2 cut(s) 145, 156
Ama87I CYCGRG 1 cut(s) 93
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 3 cut(s) 11, 176, 193
AspS9I GGNCC 2 cut(s) 91, 220
AsuHPI GGTGA 1 cut(s) 17
AvaI CYCGRG 1 cut(s) 93
AvaII GGWCC 1 cut(s) 220
BaeGI GKGCMC 1 cut(s) 61
BanII GRGCYC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 3 cut(s) 23, 163, 180
BccI CCATC 2 cut(s) 34, 259
BciT130I CCWGG 1 cut(s) 323
BcoDI GTCTC 2 cut(s) 145, 156
BisI GCNGC 3 cut(s) 12, 177, 194
BlsI GCNGC 3 cut(s) 13, 178, 195
Bme1390I CCNGG 1 cut(s) 323
Bme18I GGWCC 1 cut(s) 220
BmeT110I CYCGRG 1 cut(s) 93
BmgT120I GGNCC 2 cut(s) 91, 220
BmrFI CCNGG 1 cut(s) 323
BmsI GCATC 2 cut(s) 9, 58
BpmI CTGGAG 1 cut(s) 83
BsaJI CCNNGG 1 cut(s) 322
Bse118I RCCGGY 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 66
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 1 cut(s) 322
BseGI GGATG 1 cut(s) 73
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 1 cut(s) 66
BseRI GAGGAG 1 cut(s) 287
BseSI GKGCMC 1 cut(s) 61
BseXI GCAGC 3 cut(s) 23, 163, 180
BshFI GGCC 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 93
BsiSI CCGG 1 cut(s) 89
BsmAI GTCTC 2 cut(s) 145, 156
BsnI GGCC 1 cut(s) 92
BsoBI CYCGRG 1 cut(s) 93
Bsp1286I GDGCHC 2 cut(s) 61, 102
Bsp143I GATC 2 cut(s) 294, 346
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 1 cut(s) 283
BsrFI RCCGGY 1 cut(s) 88
BsrI ACTGG 1 cut(s) 66
BssAI RCCGGY 1 cut(s) 88
BssECI CCNNGG 1 cut(s) 322
BssMI GATC 2 cut(s) 294, 346
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 1 cut(s) 116
Bst6I CTCTTC 2 cut(s) 30, 264
BstC8I GCNNGC 2 cut(s) 86, 90
BstDEI CTNAG 2 cut(s) 245, 291
BstF5I GGATG 1 cut(s) 73
BstKTI GATC 2 cut(s) 297, 349
BstMAI GTCTC 2 cut(s) 145, 156
BstMBI GATC 2 cut(s) 294, 346
BstMWI GCNNNNNNNGC 2 cut(s) 193, 313
BstNI CCWGG 1 cut(s) 323
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 321
BstSLI GKGCMC 1 cut(s) 61
BstV1I GCAGC 3 cut(s) 23, 163, 180
BsuRI GGCC 1 cut(s) 92
BtsCI GGATG 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 59
Cac8I GCNNGC 2 cut(s) 86, 90
Cfr10I RCCGGY 1 cut(s) 88
Cfr13I GGNCC 2 cut(s) 91, 220
CseI GACGC 1 cut(s) 182
CviAII CATG 3 cut(s) 81, 155, 308
CviJI RGCY 8 cut(s) 92, 100, 187, 193, 226, 316, 340, 373
CviKI_1 RGCY 8 cut(s) 92, 100, 187, 193, 226, 316, 340, 373
DdeI CTNAG 2 cut(s) 245, 291
DpnI GATC 2 cut(s) 296, 348
DpnII GATC 2 cut(s) 294, 346
Eam1104I CTCTTC 2 cut(s) 30, 264
EarI CTCTTC 2 cut(s) 30, 264
Ecl136II GAGCTC 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 220
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 173
Eco88I CYCGRG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 102
FaeI CATG 3 cut(s) 84, 158, 311
FaiI YATR 5 cut(s) 82, 156, 170, 284, 309
FatI CATG 3 cut(s) 80, 154, 307
Fnu4HI GCNGC 3 cut(s) 12, 177, 194
FokI GGATG 1 cut(s) 80
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 3 cut(s) 12, 177, 194
GluI GCNGC 3 cut(s) 12, 177, 194
GsuI CTGGAG 1 cut(s) 83
HaeIII GGCC 1 cut(s) 92
HapII CCGG 1 cut(s) 89
HgaI GACGC 1 cut(s) 182
Hin1II CATG 3 cut(s) 84, 158, 311
HindIII AAGCTT 1 cut(s) 185
HpaII CCGG 1 cut(s) 89
HphI GGTGA 1 cut(s) 17
Hpy188I TCNGA 1 cut(s) 138
Hpy188III TCNNGA 1 cut(s) 301
HpyAV CCTTC 2 cut(s) 138, 373
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 1 cut(s) 214
HpyCH4V TGCA 4 cut(s) 11, 22, 196, 211
HpyF10VI GCNNNNNNNGC 2 cut(s) 193, 313
HpyF3I CTNAG 2 cut(s) 245, 291
HpySE526I ACGT 1 cut(s) 214
Hsp92II CATG 3 cut(s) 84, 158, 311
KroI GCCGGC 1 cut(s) 88
KroNI GCCGGC 1 cut(s) 90
Kzo9I GATC 2 cut(s) 294, 346
LmnI GCTCC 2 cut(s) 105, 337
LpnPI CCDG 6 cut(s) 47, 102, 172, 236, 308, 335
Lsp1109I GCAGC 3 cut(s) 23, 163, 180
LweI GCATC 2 cut(s) 9, 58
MaeII ACGT 1 cut(s) 214
MalI GATC 2 cut(s) 296, 348
MboI GATC 2 cut(s) 294, 346
MboII GAAGA 2 cut(s) 47, 281
MfeI CAATTG 2 cut(s) 108, 350
MhlI GDGCHC 2 cut(s) 61, 102
MluCI AATT 2 cut(s) 108, 350
MnlI CCTC 4 cut(s) 48, 58, 211, 265
MroNI GCCGGC 1 cut(s) 88
MspA1I CMGCKG 1 cut(s) 193
MspI CCGG 1 cut(s) 89
MspR9I CCNGG 1 cut(s) 323
MunI CAATTG 2 cut(s) 108, 350
MvaI CCWGG 1 cut(s) 323
MwoI GCNNNNNNNGC 2 cut(s) 193, 313
NaeI GCCGGC 1 cut(s) 90
NdeII GATC 2 cut(s) 294, 346
NgoMIV GCCGGC 1 cut(s) 88
NlaIII CATG 3 cut(s) 84, 158, 311
NspI RCATGY 1 cut(s) 158
PdiI GCCGGC 1 cut(s) 90
PkrI GCNGC 3 cut(s) 13, 178, 195
Psp124BI GAGCTC 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspPI GGNCC 2 cut(s) 91, 220
PvuII CAGCTG 1 cut(s) 193
SacI GAGCTC 1 cut(s) 102
SatI GCNGC 3 cut(s) 12, 177, 194
Sau3AI GATC 2 cut(s) 294, 346
Sau96I GGNCC 2 cut(s) 91, 220
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 2 cut(s) 61, 102
SetI ASST 7 cut(s) 102, 189, 195, 217, 342, 375, 384
SfaNI GCATC 2 cut(s) 9, 58
SinI GGWCC 1 cut(s) 220
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 2 cut(s) 108, 350
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 321
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 1 cut(s) 217
TasI AATT 2 cut(s) 108, 350
TscAI CASTG 1 cut(s) 66
TseI GCWGC 3 cut(s) 11, 176, 193
TspDTI ATGAA 1 cut(s) 324
TspRI CASTG 1 cut(s) 66
VpaK11BI GGWCC 1 cut(s) 220
XceI RCATGY 1 cut(s) 158
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.