RchiOBHm_Chr4g0420421

Inosine-uridine preferring nucleoside hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001810
Physical Location & Seq
Reverse (-)
46151251 .. 46154178
2928 bp
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UTR
Exon/CDS
Intron
PRQ39016

Sequence Viewer

Length: 663 bp
ATGCTGTTGCAGAAGCACTTTTCTTGGGGAGTGCTTGTGGTACTAGTTGTGGTTATCGGGGGTAGCTTGCAGACTGTGGTGGAGGGACGCCCTCGCCGGATTCTTTTGGATACCGACGTCGACACCGATGATTTCTTTGCTCTCTTGTACCTCTTGAAGCTCAACAGATCAGAGTTTCAGTTGGAGGCAGTAACAGTCAATGCAAATGCGTGGACCAATTCGGGGCATGCTGTGAACCATATATATGACCTCCTTTACATGATGGGACGTGATGATGTTGCTGTTGGAGTTGGAGGTGAGGGTGGTATGCTAGAAGATGGTACAATTCTCCCAAATGTTGGTGGATATCTTCCATTAATCGAACAGGGGACTACAACAGCAGGAGGATGCAGATATAGACAAGCTATTCCTGTAGGACCTGGAGGTCGATTAGATATCGATACTAATTTTGGCATCCGAAAAGCTTTTCTCCCACAGGGGAGCAGGAGATATAGTCCATTTCGACAACCTACTGCTCAGCAAGTGATGATTGACAAAATATCTGCAGGTCCCAAAATAACTGTGATTGTTATAGGAGCACATACAAACTTTGCCATATTCCTTATGAATAATCCACACTTGAAGAAGAATGTTGAGCATATTTATGTGATGGGGGTGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
IU_nuc_hydro

Protein Analysis

220

Amino Acids

24.09

Weight (kDa)

7.02

Isoelectric Point (pI)

30.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IU_nuc_hydro PF01156 34 - 218 1.3e-19 Inosine-uridine preferring nucleoside hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 423
AatII GACGTC 1 cut(s) 120
Acc36I ACCTGC 1 cut(s) 536
AccB7I CCANNNNNTGG 1 cut(s) 338
AccI GTMKAC 1 cut(s) 120
AcyI GRCGYC 2 cut(s) 88, 117
AfaI GTAC 3 cut(s) 42, 149, 322
AfiI CCNNNNNNNGG 2 cut(s) 222, 338
AgsI TTSAA 2 cut(s) 157, 622
AhlI ACTAGT 1 cut(s) 43
AjiI CACGTC 1 cut(s) 269
AjnI CCWGG 1 cut(s) 418
AluBI AGCT 4 cut(s) 66, 160, 404, 464
AluI AGCT 4 cut(s) 66, 160, 404, 464
Alw21I GWGCWC 1 cut(s) 580
AseI ATTAAT 1 cut(s) 356
AspS9I GGNCC 3 cut(s) 213, 416, 548
AsuHPI GGTGA 1 cut(s) 308
AvaII GGWCC 3 cut(s) 213, 416, 548
BaeI ACNNNNGTAYC 2 cut(s) 102, 135
Bbv12I GWGCWC 1 cut(s) 580
BccI CCATC 3 cut(s) 256, 311, 643
BciT130I CCWGG 1 cut(s) 420
BciVI GTATCC 1 cut(s) 103
BcuI ACTAGT 1 cut(s) 43
BfaI CTAG 2 cut(s) 44, 311
BfmI CTRYAG 2 cut(s) 411, 543
BfuAI ACCTGC 1 cut(s) 536
BfuI GTATCC 1 cut(s) 103
BlpI GCTNAGC 1 cut(s) 516
Bme1390I CCNGG 1 cut(s) 420
Bme18I GGWCC 3 cut(s) 213, 416, 548
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 3 cut(s) 213, 416, 548
BmiI GGNNCC 1 cut(s) 550
BmrFI CCNGG 1 cut(s) 420
BmsI GCATC 2 cut(s) 377, 462
BpmI CTGGAG 1 cut(s) 441
Bpu1102I GCTNAGC 1 cut(s) 516
Bsa29I ATCGAT 1 cut(s) 438
BsaHI GRCGYC 2 cut(s) 88, 117
BsaXI ACNNNNNCTCC 4 cut(s) 21, 51, 478, 508
Bsc4I CCNNNNNNNGG 2 cut(s) 222, 338
BseBI CCWGG 1 cut(s) 420
BseCI ATCGAT 1 cut(s) 438
BseGI GGATG 2 cut(s) 392, 453
BseLI CCNNNNNNNGG 2 cut(s) 222, 338
BseMII CTCAG 1 cut(s) 530
BshVI ATCGAT 1 cut(s) 438
BsiHKAI GWGCWC 1 cut(s) 580
BsiSI CCGG 1 cut(s) 97
BslFI GGGAC 4 cut(s) 99, 279, 382, 534
BslI CCNNNNNNNGG 2 cut(s) 222, 338
BsmFI GGGAC 4 cut(s) 99, 279, 382, 534
Bsp1286I GDGCHC 1 cut(s) 580
Bsp143I GATC 1 cut(s) 167
Bsp1720I GCTNAGC 1 cut(s) 516
BspCNI CTCAG 1 cut(s) 529
BspDI ATCGAT 1 cut(s) 438
BspLI GGNNCC 1 cut(s) 550
BspMAI CTGCAG 1 cut(s) 547
BspMI ACCTGC 1 cut(s) 536
BssMI GATC 1 cut(s) 167
BssNI GRCGYC 2 cut(s) 88, 117
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 3 cut(s) 76, 196, 562
BstACI GRCGYC 2 cut(s) 88, 117
BstC8I GCNNGC 2 cut(s) 68, 228
BstDEI CTNAG 1 cut(s) 516
BstF5I GGATG 2 cut(s) 392, 453
BstKTI GATC 1 cut(s) 170
BstMBI GATC 1 cut(s) 167
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 1 cut(s) 418
BstSFI CTRYAG 2 cut(s) 411, 543
Bsu15I ATCGAT 1 cut(s) 438
BsuI GTATCC 1 cut(s) 103
BsuTUI ATCGAT 1 cut(s) 438
BtrI CACGTC 1 cut(s) 269
BtsCI GGATG 2 cut(s) 392, 453
BveI ACCTGC 1 cut(s) 536
Cac8I GCNNGC 2 cut(s) 68, 228
Cfr13I GGNCC 3 cut(s) 213, 416, 548
ClaI ATCGAT 1 cut(s) 438
CseI GACGC 1 cut(s) 96
Csp6I GTAC 3 cut(s) 41, 148, 321
CviAII CATG 2 cut(s) 227, 259
CviJI RGCY 4 cut(s) 66, 160, 404, 464
CviKI_1 RGCY 4 cut(s) 66, 160, 404, 464
CviQI GTAC 3 cut(s) 41, 148, 321
DdeI CTNAG 1 cut(s) 516
DpnI GATC 1 cut(s) 169
DpnII GATC 1 cut(s) 167
DrdI GACNNNNNNGTC 1 cut(s) 423
DseDI GACNNNNNNGTC 1 cut(s) 423
Eco32I GATATC 2 cut(s) 347, 436
Eco47I GGWCC 3 cut(s) 213, 416, 548
EcoO109I RGGNCCY 2 cut(s) 416, 548
EcoRII CCWGG 1 cut(s) 418
EcoRV GATATC 2 cut(s) 347, 436
FaeI CATG 2 cut(s) 230, 262
FaqI GGGAC 4 cut(s) 99, 279, 382, 534
FatI CATG 2 cut(s) 226, 258
FblI GTMKAC 1 cut(s) 120
FokI GGATG 2 cut(s) 399, 440
FspBI CTAG 2 cut(s) 44, 311
GsuI CTGGAG 1 cut(s) 441
HapII CCGG 1 cut(s) 97
HgaI GACGC 1 cut(s) 96
Hin1I GRCGYC 2 cut(s) 88, 117
Hin1II CATG 2 cut(s) 230, 262
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HindIII AAGCTT 1 cut(s) 462
HinfI GANTC 1 cut(s) 100
HpaII CCGG 1 cut(s) 97
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 3 cut(s) 121, 213, 235
Hpy188I TCNGA 2 cut(s) 172, 458
Hpy188III TCNNGA 1 cut(s) 154
Hpy8I GTNNAC 3 cut(s) 121, 213, 235
Hpy99I CGWCG 2 cut(s) 119, 122
HpyCH4III ACNGT 3 cut(s) 76, 196, 562
HpyCH4IV ACGT 2 cut(s) 117, 268
HpyCH4V TGCA 5 cut(s) 10, 70, 203, 390, 545
HpyF3I CTNAG 1 cut(s) 516
HpySE526I ACGT 2 cut(s) 117, 268
Hsp92I GRCGYC 2 cut(s) 88, 117
Hsp92II CATG 2 cut(s) 230, 262
Kzo9I GATC 1 cut(s) 167
LmnI GCTCC 2 cut(s) 480, 575
LpnPI CCDG 9 cut(s) 110, 350, 366, 405, 423, 432, 461, 469, 531
LweI GCATC 2 cut(s) 377, 462
MaeI CTAG 2 cut(s) 44, 311
MaeII ACGT 2 cut(s) 117, 268
MaeIII GTNAC 1 cut(s) 190
MalI GATC 1 cut(s) 169
MboI GATC 1 cut(s) 167
MboII GAAGA 4 cut(s) 326, 341, 634, 637
MhlI GDGCHC 1 cut(s) 580
MluCI AATT 3 cut(s) 217, 324, 445
MmeI TCCRAC 3 cut(s) 162, 265, 271
MnlI CCTC 9 cut(s) 76, 102, 161, 178, 260, 287, 292, 377, 416
MseI TTAA 1 cut(s) 356
MslI CAYNNNNRTG 2 cut(s) 243, 642
MspI CCGG 1 cut(s) 97
MspR9I CCNGG 1 cut(s) 420
MvaI CCWGG 1 cut(s) 420
NdeII GATC 1 cut(s) 167
NlaIII CATG 2 cut(s) 230, 262
NlaIV GGNNCC 1 cut(s) 550
NspI RCATGY 1 cut(s) 230
PaeI GCATGC 1 cut(s) 230
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 1 cut(s) 100
PflMI CCANNNNNTGG 1 cut(s) 338
PpuMI RGGWCCY 2 cut(s) 416, 548
PshBI ATTAAT 1 cut(s) 356
Psp5II RGGWCCY 2 cut(s) 416, 548
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 1 cut(s) 550
PspPI GGNCC 3 cut(s) 213, 416, 548
PspPPI RGGWCCY 2 cut(s) 416, 548
PstI CTGCAG 1 cut(s) 547
RsaI GTAC 3 cut(s) 42, 149, 322
RsaNI GTAC 3 cut(s) 41, 148, 321
RseI CAYNNNNRTG 2 cut(s) 243, 642
SalI GTCGAC 1 cut(s) 119
SaqAI TTAA 1 cut(s) 356
Sau3AI GATC 1 cut(s) 167
Sau96I GGNCC 3 cut(s) 213, 416, 548
ScrFI CCNGG 1 cut(s) 420
SduI GDGCHC 1 cut(s) 580
SfaNI GCATC 2 cut(s) 377, 462
SfcI CTRYAG 2 cut(s) 411, 543
SinI GGWCC 3 cut(s) 213, 416, 548
SmiMI CAYNNNNRTG 2 cut(s) 243, 642
SpeI ACTAGT 1 cut(s) 43
SphI GCATGC 1 cut(s) 230
Sse9I AATT 3 cut(s) 217, 324, 445
SspMI CTAG 2 cut(s) 44, 311
StyD4I CCNGG 1 cut(s) 418
TaaI ACNGT 3 cut(s) 76, 196, 562
TaiI ACGT 2 cut(s) 120, 271
TaqI TCGA 5 cut(s) 120, 360, 427, 438, 502
TasI AATT 3 cut(s) 217, 324, 445
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 1 cut(s) 356
Tru9I TTAA 1 cut(s) 356
TspDTI ATGAA 1 cut(s) 620
Van91I CCANNNNNTGG 1 cut(s) 338
VpaK11BI GGWCC 3 cut(s) 213, 416, 548
VspI ATTAAT 1 cut(s) 356
XceI RCATGY 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 120
XspI CTAG 2 cut(s) 44, 311
ZraI GACGTC 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.