RchiOBHm_Chr3g0453471

Inosine-uridine preferring nucleoside hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001810
Physical Location & Seq
Forward (+)
3886876 .. 3893403
6528 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42055

Sequence Viewer

Length: 1578 bp
ATGAATGAAATAACACAGGGTGGGAGAAAATATGTACCTGTAGAACAAGCTACAGCTCAACTAGTGATGAAAGATGCAGTGTCTGCAGGTTCCATTAGTGTGTTTGTTCTAGGCACACATACAAATTTAGCAATTTTTCTTATGACTAATCCTCACCTTAAGAAAAATATTGAGCATATATATGTCATGGGAGGTGCTATTGAACCTGGCATCGGTAATTTGTTCCCCCCAGACTCCAATCCATATGCGGAGTTCAATTTCTTTGGAGATCCTTTTGCTGCATACACGGTATTGCATTCTGGCGTTCCAATCACACTCATTCCTTTAGATGCGACAAGGACGATTCCTGTAAACAAGAATTTCTTTGCTGCATTTGAACAGAAACAAGATACAATATTTGAGGCACAATATAGCTTCCAGACCTTGAAAATGGTTCGTGATACATGGCCTAACAATAAGTTTCATGAGGAATATTGCTTCTGGGATTCTTTTATGGTTGGTGTTGCGCTGTCTCAAATGAGTAACTTAGAGAGATCTGATCAAGATCATGGAGGAGAAAATGAGTTTGCGGAAATGGAATATATGAACATCTCAGTGGTTACTTCAAATACACCTTATGGAATCTCAGATGGCTCAAATCCCCTCATCAGTGGGGGTTTGATCCCAAAGTTCAATTTACAGAAGAATGGAGTGCATAGTGGTCATGTTCAGCTAGGTTTGCAAGACCCATTTTGCCTTGTCAAGGGGGGTAAAGGAAAATGTCAGGATGGTTACACACAGAAGGCAACTGGTCCGGAAGCCGTTTCTGTTCTTGTTGCAACAGAAGCTAAACCAAATTGTGACATTGACGAGAAGATGCTTACGAAAAAGTTCTATAAAAGTTTCTTGGAAGCTGAGATTGAAGAAGGTTTCACAAAGAAATTCTGGATTGGAACTGTCAACGGCAACTATGGAAGGGTAGAACTGGAGCTGATCAAGGGTTTAGAACTTCATCTTGTTGCCAATGGAATACTAGTTACTCCAAATGGGTGGGCAACTGCTGCAACCATAGAAATTCTATATGACATACTGCATATGATGGAGCGTGATGATATTCAAGTTGGTCTTGGAAATGTGTTTGCAGTTGGACAATCTTATTCATCTGTGACTTTCACTTCGCTCGGAGACTGCAGCTACTCTAAGTCCATTCCTCAAGGCAGCGGTGGCTCCTTGGACTATGATACACTCTATGGATTTGCTCGTGACTTGCCTCGTAGTCCTAGAAGATACAAACCAGAAATTAGAGGAAGCCCTGGATTTGGACAACCGACTGCACTAGATGTTTGGAAGTCCATCGAAGTATTTATCGTTGGGGGACACATTGTGGATGAAAATGATAATGATAATGCAAGAGGGAATGTTTTCACTGTTGCTTCAAATGAATATGCAGACTTTAACATGTTTCTTGACCCTTTGGCTGCAAAAGCAGTTTTCGATTCCAAACTGAACATTACAATGATTCCATGGAAAACCCAAAAGCAAGTCAGTACGTTCTTTCCAGAAAATTCTCAATACATTGCAACAGCAGCTAACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IU_nuc_hydro

Protein Analysis

525

Amino Acids

57.94

Weight (kDa)

5.14

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IU_nuc_hydro PF01156 16 - 203 4.9e-28 Inosine-uridine preferring nucleoside hydrolase
IU_nuc_hydro PF01156 446 - 515 1.9e-09 Inosine-uridine preferring nucleoside hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 77
AccIII TCCGGA 1 cut(s) 793
AciI CCGC 3 cut(s) 248, 569, 1200
AclWI GGATC 2 cut(s) 263, 655
AcsI RAATTY 5 cut(s) 124, 358, 920, 1053, 1543
AdeI CACNNNGTG 2 cut(s) 20, 1363
AfaI GTAC 2 cut(s) 36, 1528
AfiI CCNNNNNNNGG 3 cut(s) 212, 742, 1298
AflII CTTAAG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 1437
AgsI TTSAA 9 cut(s) 203, 256, 377, 427, 606, 673, 902, 1097, 1416
AhlI ACTAGT 2 cut(s) 61, 1012
AjnI CCWGG 2 cut(s) 205, 1291
AluBI AGCT 9 cut(s) 50, 56, 414, 712, 827, 893, 970, 1173, 1568
AluI AGCT 9 cut(s) 50, 56, 414, 712, 827, 893, 970, 1173, 1568
Alw26I GTCTC 2 cut(s) 516, 1158
AlwI GGATC 2 cut(s) 263, 655
AlwNI CAGNNNCTG 1 cut(s) 83
Aor13HI TCCGGA 1 cut(s) 793
AoxI GGCC 1 cut(s) 446
ApeKI GCWGC 7 cut(s) 278, 368, 1040, 1170, 1197, 1457, 1565
ApoI RAATTY 5 cut(s) 124, 358, 920, 1053, 1543
ArsI GACNNNNNNTTYG 2 cut(s) 1138, 1170
Asp700I GAANNNNTTC 2 cut(s) 869, 1400
AspLEI GCGC 1 cut(s) 508
AspS9I GGNCC 1 cut(s) 791
AsuHPI GGTGA 1 cut(s) 146
AvaII GGWCC 1 cut(s) 791
BauI CACGAG 1 cut(s) 1239
BbvI GCAGC 6 cut(s) 265, 355, 1027, 1182, 1209, 1444
BccI CCATC 4 cut(s) 623, 761, 1072, 1340
BceAI ACGGC 2 cut(s) 785, 958
BciT130I CCWGG 2 cut(s) 207, 1293
BclI TGATCA 2 cut(s) 538, 972
BcoDI GTCTC 2 cut(s) 516, 1158
BcuI ACTAGT 2 cut(s) 61, 1012
BfaI CTAG 6 cut(s) 62, 110, 713, 1013, 1260, 1316
BfmI CTRYAG 4 cut(s) 39, 51, 84, 1168
BfrI CTTAAG 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 77
BglII AGATCT 1 cut(s) 533
BisI GCNGC 7 cut(s) 279, 369, 1041, 1171, 1198, 1458, 1566
BlsI GCNGC 7 cut(s) 280, 370, 1042, 1172, 1199, 1459, 1567
Bme1390I CCNGG 2 cut(s) 207, 1293
Bme18I GGWCC 1 cut(s) 791
BmgT120I GGNCC 1 cut(s) 791
BmiI GGNNCC 2 cut(s) 91, 1207
BmrFI CCNGG 2 cut(s) 207, 1293
BmsI GCATC 4 cut(s) 64, 219, 319, 846
BpmI CTGGAG 1 cut(s) 986
BpuEI CTTGAG 1 cut(s) 1176
BsaBI GATNNNNATC 1 cut(s) 543
BsaJI CCNNGG 3 cut(s) 1209, 1291, 1502
BsaWI WCCGGW 1 cut(s) 793
Bsc4I CCNNNNNNNGG 3 cut(s) 212, 742, 1298
Bse1I ACTGG 2 cut(s) 793, 969
Bse3DI GCAATG 1 cut(s) 1554
Bse8I GATNNNNATC 1 cut(s) 543
BseAI TCCGGA 1 cut(s) 793
BseBI CCWGG 2 cut(s) 207, 1293
BseDI CCNNGG 3 cut(s) 1209, 1291, 1502
BseGI GGATG 2 cut(s) 772, 1372
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 3 cut(s) 212, 742, 1298
BseMI GCAATG 1 cut(s) 1554
BseMII CTCAG 3 cut(s) 606, 639, 885
BseNI ACTGG 2 cut(s) 793, 969
BseRI GAGGAG 1 cut(s) 567
BseXI GCAGC 6 cut(s) 265, 355, 1027, 1182, 1209, 1444
BsgI GTGCAG 1 cut(s) 1296
BshFI GGCC 1 cut(s) 448
BsiSI CCGG 1 cut(s) 794
BslFI GGGAC 1 cut(s) 1368
BslI CCNNNNNNNGG 3 cut(s) 212, 742, 1298
BsmAI GTCTC 2 cut(s) 516, 1158
BsmFI GGGAC 1 cut(s) 1368
BsmI GAATGC 1 cut(s) 295
BsnI GGCC 1 cut(s) 448
Bsp13I TCCGGA 1 cut(s) 793
Bsp143I GATC 6 cut(s) 268, 533, 538, 544, 660, 972
Bsp19I CCATGG 1 cut(s) 1502
BspACI CCGC 3 cut(s) 248, 569, 1200
BspANI GGCC 1 cut(s) 448
BspCNI CTCAG 3 cut(s) 605, 638, 886
BspEI TCCGGA 1 cut(s) 793
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 2 cut(s) 91, 1207
BspMAI CTGCAG 2 cut(s) 88, 1172
BspMI ACCTGC 1 cut(s) 77
BspPI GGATC 2 cut(s) 263, 655
BspTI CTTAAG 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 1554
BsrI ACTGG 2 cut(s) 793, 969
BssECI CCNNGG 3 cut(s) 1209, 1291, 1502
BssMI GATC 6 cut(s) 268, 533, 538, 544, 660, 972
BssSI CACGAG 1 cut(s) 1239
BssT1I CCWWGG 2 cut(s) 1209, 1502
Bst2BI CACGAG 1 cut(s) 1239
Bst2UI CCWGG 2 cut(s) 207, 1293
Bst4CI ACNGT 3 cut(s) 289, 937, 1408
BstAFI CTTAAG 1 cut(s) 158
BstAPI GCANNNNNTGC 2 cut(s) 83, 1040
BstDEI CTNAG 5 cut(s) 526, 592, 625, 894, 1179
BstDSI CCRYGG 1 cut(s) 1502
BstENI CCTNNNNNAGG 1 cut(s) 740
BstF5I GGATG 2 cut(s) 772, 1372
BstHHI GCGC 1 cut(s) 508
BstKTI GATC 6 cut(s) 271, 536, 541, 547, 663, 975
BstMAI GTCTC 2 cut(s) 516, 1158
BstMBI GATC 6 cut(s) 268, 533, 538, 544, 660, 972
BstMWI GCNNNNNNNGC 7 cut(s) 83, 718, 824, 1040, 1203, 1463, 1565
BstNI CCWGG 2 cut(s) 207, 1293
BstNSI RCATGY 1 cut(s) 1441
BstSCI CCNGG 2 cut(s) 205, 1291
BstSFI CTRYAG 4 cut(s) 39, 51, 84, 1168
BstV1I GCAGC 6 cut(s) 265, 355, 1027, 1182, 1209, 1444
BstX2I RGATCY 2 cut(s) 268, 533
BstXI CCANNNNNNTGG 1 cut(s) 1029
BstYI RGATCY 2 cut(s) 268, 533
BsuRI GGCC 1 cut(s) 448
BtgI CCRYGG 1 cut(s) 1502
BtsCI GGATG 2 cut(s) 772, 1372
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 4 cut(s) 84, 600, 655, 1404
BveI ACCTGC 1 cut(s) 77
CaiI CAGNNNCTG 1 cut(s) 83
CciI TCATGA 1 cut(s) 463
CfoI GCGC 1 cut(s) 508
Cfr13I GGNCC 1 cut(s) 791
Csp6I GTAC 2 cut(s) 35, 1527
CviAII CATG 7 cut(s) 187, 444, 464, 548, 704, 1438, 1503
CviQI GTAC 2 cut(s) 35, 1527
DdeI CTNAG 5 cut(s) 526, 592, 625, 894, 1179
DpnI GATC 6 cut(s) 270, 535, 540, 546, 662, 974
DpnII GATC 6 cut(s) 268, 533, 538, 544, 660, 972
DraIII CACNNNGTG 2 cut(s) 20, 1363
Eco130I CCWWGG 2 cut(s) 1209, 1502
Eco47I GGWCC 1 cut(s) 791
EcoNI CCTNNNNNAGG 1 cut(s) 740
EcoRII CCWGG 2 cut(s) 205, 1291
EcoT14I CCWWGG 2 cut(s) 1209, 1502
ErhI CCWWGG 2 cut(s) 1209, 1502
FaeI CATG 7 cut(s) 190, 447, 467, 551, 707, 1441, 1506
FalI AAGNNNNNCTT 4 cut(s) 347, 379, 1089, 1121
FaqI GGGAC 1 cut(s) 1368
FatI CATG 7 cut(s) 186, 443, 463, 547, 703, 1437, 1502
FauNDI CATATG 2 cut(s) 244, 1074
FbaI TGATCA 2 cut(s) 538, 972
Fnu4HI GCNGC 7 cut(s) 279, 369, 1041, 1171, 1198, 1458, 1566
FokI GGATG 2 cut(s) 779, 1379
Fsp4HI GCNGC 7 cut(s) 279, 369, 1041, 1171, 1198, 1458, 1566
FspBI CTAG 6 cut(s) 62, 110, 713, 1013, 1260, 1316
GlaI GCGC 1 cut(s) 507
GluI GCNGC 7 cut(s) 279, 369, 1041, 1171, 1198, 1458, 1566
GsuI CTGGAG 1 cut(s) 986
HaeIII GGCC 1 cut(s) 448
HapII CCGG 1 cut(s) 794
HhaI GCGC 1 cut(s) 508
Hin1II CATG 7 cut(s) 190, 447, 467, 551, 707, 1441, 1506
Hin6I GCGC 1 cut(s) 506
HinP1I GCGC 1 cut(s) 506
HincII GTYRAC 1 cut(s) 940
HindII GTYRAC 1 cut(s) 940
HinfI GANTC 6 cut(s) 233, 343, 485, 621, 1475, 1498
HpaII CCGG 1 cut(s) 794
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 2 cut(s) 352, 940
Hpy188I TCNGA 3 cut(s) 538, 628, 1163
Hpy8I GTNNAC 2 cut(s) 352, 940
HpyAV CCTTC 3 cut(s) 775, 899, 948
HpyCH4III ACNGT 3 cut(s) 289, 937, 1408
HpyCH4IV ACGT 1 cut(s) 1529
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 718, 824, 1040, 1203, 1463, 1565
HpyF3I CTNAG 5 cut(s) 526, 592, 625, 894, 1179
HpySE526I ACGT 1 cut(s) 1529
Hsp92II CATG 7 cut(s) 190, 447, 467, 551, 707, 1441, 1506
HspAI GCGC 1 cut(s) 506
Kpn2I TCCGGA 1 cut(s) 793
Ksp22I TGATCA 2 cut(s) 538, 972
Kzo9I GATC 6 cut(s) 268, 533, 538, 544, 660, 972
LmnI GCTCC 3 cut(s) 967, 1081, 1211
Lsp1109I GCAGC 6 cut(s) 265, 355, 1027, 1182, 1209, 1444
LweI GCATC 4 cut(s) 64, 219, 319, 846
MaeI CTAG 6 cut(s) 62, 110, 713, 1013, 1260, 1316
MaeII ACGT 1 cut(s) 1529
MaeIII GTNAC 7 cut(s) 521, 598, 770, 839, 1015, 1144, 1241
MalI GATC 6 cut(s) 270, 535, 540, 546, 662, 974
MboI GATC 6 cut(s) 268, 533, 538, 544, 660, 972
MboII GAAGA 4 cut(s) 694, 865, 914, 1275
MflI RGATCY 2 cut(s) 268, 533
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 1105
MroI TCCGGA 1 cut(s) 793
MroXI GAANNNNTTC 2 cut(s) 869, 1400
MseI TTAA 2 cut(s) 159, 1434
MslI CAYNNNNRTG 4 cut(s) 98, 180, 593, 1493
MspA1I CMGCKG 1 cut(s) 1200
MspCI CTTAAG 1 cut(s) 158
MspI CCGG 1 cut(s) 794
MspR9I CCNGG 2 cut(s) 207, 1293
Mva1269I GAATGC 1 cut(s) 295
MvaI CCWGG 2 cut(s) 207, 1293
MwoI GCNNNNNNNGC 7 cut(s) 83, 718, 824, 1040, 1203, 1463, 1565
NcoI CCATGG 1 cut(s) 1502
NdeI CATATG 2 cut(s) 244, 1074
NdeII GATC 6 cut(s) 268, 533, 538, 544, 660, 972
NlaIII CATG 7 cut(s) 190, 447, 467, 551, 707, 1441, 1506
NlaIV GGNNCC 2 cut(s) 91, 1207
NmuCI GTSAC 3 cut(s) 839, 1144, 1241
NspI RCATGY 1 cut(s) 1441
PagI TCATGA 1 cut(s) 463
PciI ACATGT 1 cut(s) 1437
PctI GAATGC 1 cut(s) 295
PdmI GAANNNNTTC 2 cut(s) 869, 1400
PfeI GAWTC 5 cut(s) 343, 485, 621, 1475, 1498
PkrI GCNGC 7 cut(s) 280, 370, 1042, 1172, 1199, 1459, 1567
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PscI ACATGT 1 cut(s) 1437
Psp6I CCWGG 2 cut(s) 205, 1291
PspGI CCWGG 2 cut(s) 205, 1291
PspN4I GGNNCC 2 cut(s) 91, 1207
PspPI GGNCC 1 cut(s) 791
PstI CTGCAG 2 cut(s) 88, 1172
PstNI CAGNNNCTG 1 cut(s) 83
PsuI RGATCY 2 cut(s) 268, 533
RsaI GTAC 2 cut(s) 36, 1528
RsaNI GTAC 2 cut(s) 35, 1527
RseI CAYNNNNRTG 4 cut(s) 98, 180, 593, 1493
SaqAI TTAA 2 cut(s) 159, 1434
SatI GCNGC 7 cut(s) 279, 369, 1041, 1171, 1198, 1458, 1566
Sau3AI GATC 6 cut(s) 268, 533, 538, 544, 660, 972
Sau96I GGNCC 1 cut(s) 791
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 2 cut(s) 207, 1293
SfaNI GCATC 4 cut(s) 64, 219, 319, 846
SfcI CTRYAG 4 cut(s) 39, 51, 84, 1168
SinI GGWCC 1 cut(s) 791
SmiMI CAYNNNNRTG 4 cut(s) 98, 180, 593, 1493
SmlI CTYRAG 2 cut(s) 158, 1191
SmoI CTYRAG 2 cut(s) 158, 1191
SpeI ACTAGT 2 cut(s) 61, 1012
SsiI CCGC 3 cut(s) 248, 569, 1200
SspI AATATT 3 cut(s) 169, 396, 473
SspMI CTAG 6 cut(s) 62, 110, 713, 1013, 1260, 1316
StyD4I CCNGG 2 cut(s) 205, 1291
StyI CCWWGG 2 cut(s) 1209, 1502
TaaI ACNGT 3 cut(s) 289, 937, 1408
TaiI ACGT 1 cut(s) 1532
TaqI TCGA 2 cut(s) 1335, 1473
TfiI GAWTC 5 cut(s) 343, 485, 621, 1475, 1498
Tru1I TTAA 2 cut(s) 159, 1434
Tru9I TTAA 2 cut(s) 159, 1434
TscAI CASTG 4 cut(s) 84, 600, 655, 1411
TseFI GTSAC 3 cut(s) 839, 1144, 1241
TseI GCWGC 7 cut(s) 278, 368, 1040, 1170, 1197, 1457, 1565
Tsp45I GTSAC 3 cut(s) 839, 1144, 1241
TspDTI ATGAA 9 cut(s) 17, 21, 83, 452, 599, 980, 1128, 1383, 1434
TspRI CASTG 4 cut(s) 84, 600, 655, 1411
Vha464I CTTAAG 1 cut(s) 158
VpaK11BI GGWCC 1 cut(s) 791
XagI CCTNNNNNAGG 1 cut(s) 740
XapI RAATTY 5 cut(s) 124, 358, 920, 1053, 1543
XceI RCATGY 1 cut(s) 1441
XmnI GAANNNNTTC 2 cut(s) 869, 1400
XspI CTAG 6 cut(s) 62, 110, 713, 1013, 1260, 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.