AT5G19170

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011824
Physical Location & Seq
Forward (+)
6445245 .. 6447419
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G19170.1

Sequence Viewer

Length: 1176 bp
ATGGAGAGCTTGACGATGCTCCTTATCATGGGACACTTCATACAACTGGGAGTTTCAGTTATTCCAGTTTCTACCATTTATAGTCCTGACGGCGATTTGATTGATTGTATCTACCGGACGGATCAACGAGCCTTCGACCACCCACTCTTACGAAACCATATTATTCAGGAATATCCTACCAACATGCCACAAATAGAAAAAAGCGTACAAGAAAATTGGCAAATTTGGCACGACACCGGAGAAAAGTGTCCGGCAGAGACGATTCCTATGCGTCGGGAATTGAATCCAGTTTCTCACCAAAAGCAGCATGTACATGTTCCAAAAGAGTATGAGGTCGCCAACAGAGCAACTTCAGGGCACAAGTATGCAATTGCATACATGATTAACCGGACAAATGTGTACGGAACAAGGGCAACGTTTAATGTCTGGACACCAAAGGTAGAATCGGCTTACGATTTTAGCCTCGCTCAGATTTGGCTGGCATCTGGGTCATACGAGACTGCGGATCTCAATACTGTTGAAGCCGGTTGGCAGGTTTCTCGGTCGAGGTATAATGACAGCCAGGCCAGATTGTTTACATATTGGACGGCGGATGGATATAACAAGACCGGGTGTTATGGCCTCGACCTTCCTGGATTTGTGCAGACTAGCAGTACCATCGCCATCGGAGCTGCCATTGGTCGCACCTCGACATATGGAGGCACCCAGTTTGACATGACACTTCAGATCTGGAAGGATCTGTTTTCAGGACATTGGTGGCTGGCTCTGGGCCCAAATTTAGTGCCCATTGGATACTGGCCGGCCGTAATTTTCACGACCTTATCCGATTATGCGACGGCAGTTGAATGGGGAGGCGAAGTATTATACCGAAACCTGTCGAGCGTGAATACGGTGGCTCAAATGGGTTCAGGAGAATATGCCGACAAAGGCTATGGGAAAGCGGCATATTTCTGCAATCTGAAGGTGGCAGAGAACAACCACACCCTTCTCCCGGTTCAAGATTTCGGCGTTCAAGCCGATTATCCAGAATATTATACCGTTAAGAAGTCATCTAACCGGAACTGCGGAAACCATTTTTATTTCGGCGGACCAGGACCTCAACGTTCAGAAGCAGTTCGGGGAACAACGGTTTTTGGTTTTTGCTTTTTATTTTATTTGAGTGTGTTTTGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

391

Amino Acids

44.11

Weight (kDa)

5.99

Isoelectric Point (pI)

30.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 25 - 126 1.6e-23 Neprosin activation peptide
Neprosin PF03080 152 - 364 1.3e-72 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 523
AccB1I GGYRCC 1 cut(s) 701
AciI CCGC 5 cut(s) 503, 590, 941, 1065, 1086
AclI AACGTT 2 cut(s) 416, 1102
AclWI GGATC 3 cut(s) 129, 513, 744
AcoI YGGCCR 2 cut(s) 797, 801
AcsI RAATTY 2 cut(s) 222, 775
AcuI CTGAAG 3 cut(s) 336, 707, 980
AfaI GTAC 4 cut(s) 207, 312, 401, 655
AfiI CCNNNNNNNGG 2 cut(s) 28, 991
AflIII ACRYGT 1 cut(s) 313
AgsI TTSAA 5 cut(s) 283, 521, 845, 998, 1013
AjnI CCWGG 3 cut(s) 561, 631, 1090
AluBI AGCT 2 cut(s) 9, 671
AluI AGCT 2 cut(s) 9, 671
Alw26I GTCTC 2 cut(s) 251, 491
AlwI GGATC 3 cut(s) 129, 513, 744
AoxI GGCC 5 cut(s) 564, 619, 769, 797, 801
ApaI GGGCCC 1 cut(s) 773
ApeKI GCWGC 2 cut(s) 304, 671
ApoI RAATTY 2 cut(s) 222, 775
Asp700I GAANNNNTTC 1 cut(s) 1113
AspS9I GGNCC 4 cut(s) 769, 770, 1088, 1094
AsuC2I CCSGG 2 cut(s) 610, 992
AsuHPI GGTGA 1 cut(s) 287
AvaII GGWCC 2 cut(s) 1088, 1094
BaeGI GKGCMC 3 cut(s) 360, 773, 786
BanI GGYRCC 1 cut(s) 701
BanII GRGCYC 1 cut(s) 773
BbvI GCAGC 2 cut(s) 316, 658
BccI CCATC 3 cut(s) 587, 665, 671
BceAI ACGGC 4 cut(s) 106, 603, 788, 852
BcgI CGANNNNNNTGC 6 cut(s) 250, 284, 521, 555, 640, 674
BciT130I CCWGG 3 cut(s) 563, 633, 1092
BciVI GTATCC 1 cut(s) 785
BcnI CCSGG 2 cut(s) 610, 992
BcoDI GTCTC 2 cut(s) 251, 491
BfaI CTAG 1 cut(s) 648
BfuAI ACCTGC 1 cut(s) 523
BfuI GTATCC 1 cut(s) 785
BglII AGATCT 1 cut(s) 726
BisI GCNGC 3 cut(s) 305, 672, 942
BlsI GCNGC 3 cut(s) 306, 673, 943
Bme1390I CCNGG 5 cut(s) 563, 610, 633, 992, 1092
Bme18I GGWCC 2 cut(s) 1088, 1094
BmgT120I GGNCC 4 cut(s) 769, 770, 1088, 1094
BmiI GGNNCC 2 cut(s) 703, 771
BmrFI CCNGG 5 cut(s) 563, 610, 633, 992, 1092
BmrI ACTGGG 2 cut(s) 56, 700
BmsI GCATC 2 cut(s) 6, 491
BmuI ACTGGG 2 cut(s) 56, 700
BpuMI CCSGG 2 cut(s) 610, 992
BsaWI WCCGGW 4 cut(s) 114, 236, 387, 1056
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 843, 873
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 991
Bse118I RCCGGY 2 cut(s) 524, 799
Bse1I ACTGG 5 cut(s) 51, 65, 287, 706, 800
BseBI CCWGG 3 cut(s) 563, 633, 1092
BseGI GGATG 1 cut(s) 598
BseLI CCNNNNNNNGG 2 cut(s) 28, 991
BseMII CTCAG 1 cut(s) 482
BseNI ACTGG 5 cut(s) 51, 65, 287, 706, 800
BseSI GKGCMC 3 cut(s) 360, 773, 786
BseX3I CGGCCG 1 cut(s) 801
BseXI GCAGC 2 cut(s) 316, 658
BsgI GTGCAG 1 cut(s) 662
Bsh1285I CGRYCG 2 cut(s) 545, 804
BshFI GGCC 5 cut(s) 566, 621, 771, 799, 803
BshNI GGYRCC 1 cut(s) 701
BsiEI CGRYCG 2 cut(s) 545, 804
BsiSI CCGG 9 cut(s) 115, 237, 251, 388, 525, 609, 800, 992, 1057
BslFI GGGAC 1 cut(s) 45
BslI CCNNNNNNNGG 2 cut(s) 28, 991
BsmAI GTCTC 2 cut(s) 251, 491
BsmBI CGTCTC 1 cut(s) 251
BsmFI GGGAC 1 cut(s) 45
BsnI GGCC 5 cut(s) 566, 621, 771, 799, 803
Bsp120I GGGCCC 1 cut(s) 769
Bsp1286I GDGCHC 3 cut(s) 360, 773, 786
Bsp1407I TGTACA 1 cut(s) 310
Bsp143I GATC 4 cut(s) 121, 505, 726, 736
BspACI CCGC 5 cut(s) 503, 590, 941, 1065, 1086
BspANI GGCC 5 cut(s) 566, 621, 771, 799, 803
BspCNI CTCAG 1 cut(s) 481
BspLI GGNNCC 2 cut(s) 703, 771
BspMI ACCTGC 1 cut(s) 523
BspPI GGATC 3 cut(s) 129, 513, 744
BspT107I GGYRCC 1 cut(s) 701
BsrFI RCCGGY 2 cut(s) 524, 799
BsrGI TGTACA 1 cut(s) 310
BsrI ACTGG 5 cut(s) 51, 65, 287, 706, 800
BssAI RCCGGY 2 cut(s) 524, 799
BssMI GATC 4 cut(s) 121, 505, 726, 736
Bst2UI CCWGG 3 cut(s) 563, 633, 1092
Bst4CI ACNGT 4 cut(s) 517, 892, 1039, 1129
BstAUI TGTACA 1 cut(s) 310
BstC8I GCNNGC 3 cut(s) 480, 762, 801
BstDEI CTNAG 1 cut(s) 468
BstF5I GGATG 1 cut(s) 598
BstKTI GATC 4 cut(s) 124, 508, 729, 739
BstMAI GTCTC 2 cut(s) 251, 491
BstMBI GATC 4 cut(s) 121, 505, 726, 736
BstMCI CGRYCG 2 cut(s) 545, 804
BstMWI GCNNNNNNNGC 3 cut(s) 226, 344, 668
BstNI CCWGG 3 cut(s) 563, 633, 1092
BstNSI RCATGY 3 cut(s) 187, 311, 317
BstSCI CCNGG 5 cut(s) 561, 608, 631, 990, 1090
BstSLI GKGCMC 3 cut(s) 360, 773, 786
BstV1I GCAGC 2 cut(s) 316, 658
BstX2I RGATCY 3 cut(s) 505, 726, 736
BstYI RGATCY 3 cut(s) 505, 726, 736
BstZI CGGCCG 1 cut(s) 801
BsuI GTATCC 1 cut(s) 785
BsuRI GGCC 5 cut(s) 566, 621, 771, 799, 803
BtgZI GCGATG 1 cut(s) 643
BtsCI GGATG 1 cut(s) 598
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 3 cut(s) 480, 762, 801
Cfr10I RCCGGY 2 cut(s) 524, 799
Cfr13I GGNCC 4 cut(s) 769, 770, 1088, 1094
CseI GACGC 1 cut(s) 260
Csp6I GTAC 4 cut(s) 206, 311, 400, 654
CviAII CATG 6 cut(s) 28, 184, 308, 314, 379, 715
CviQI GTAC 4 cut(s) 206, 311, 400, 654
DdeI CTNAG 1 cut(s) 468
DpnI GATC 4 cut(s) 123, 507, 728, 738
DpnII GATC 4 cut(s) 121, 505, 726, 736
EaeI YGGCCR 2 cut(s) 797, 801
EagI CGGCCG 1 cut(s) 801
EciI GGCGGA 2 cut(s) 605, 1101
EclXI CGGCCG 1 cut(s) 801
Eco24I GRGCYC 1 cut(s) 773
Eco47I GGWCC 2 cut(s) 1088, 1094
Eco52I CGGCCG 1 cut(s) 801
Eco57I CTGAAG 3 cut(s) 336, 707, 980
EcoO109I RGGNCCY 1 cut(s) 1094
EcoRII CCWGG 3 cut(s) 561, 631, 1090
EcoT38I GRGCYC 1 cut(s) 773
Esp3I CGTCTC 1 cut(s) 251
FaeI CATG 6 cut(s) 31, 187, 311, 317, 382, 718
FaqI GGGAC 1 cut(s) 45
FatI CATG 6 cut(s) 27, 183, 307, 313, 378, 714
FauNDI CATATG 1 cut(s) 694
Fnu4HI GCNGC 3 cut(s) 305, 672, 942
FokI GGATG 1 cut(s) 605
FriOI GRGCYC 1 cut(s) 773
FseI GGCCGGCC 1 cut(s) 803
Fsp4HI GCNGC 3 cut(s) 305, 672, 942
FspBI CTAG 1 cut(s) 648
GluI GCNGC 3 cut(s) 305, 672, 942
HaeIII GGCC 5 cut(s) 566, 621, 771, 799, 803
HapII CCGG 9 cut(s) 115, 237, 251, 388, 525, 609, 800, 992, 1057
HgaI GACGC 1 cut(s) 260
Hin1II CATG 6 cut(s) 31, 187, 311, 317, 382, 718
HinfI GANTC 3 cut(s) 262, 283, 443
HpaII CCGG 9 cut(s) 115, 237, 251, 388, 525, 609, 800, 992, 1057
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 2 cut(s) 400, 576
Hpy188I TCNGA 6 cut(s) 471, 668, 726, 826, 960, 1108
Hpy8I GTNNAC 2 cut(s) 400, 576
Hpy99I CGWCG 2 cut(s) 276, 838
HpyAV CCTTC 5 cut(s) 142, 638, 727, 955, 995
HpyCH4III ACNGT 4 cut(s) 517, 892, 1039, 1129
HpyCH4IV ACGT 2 cut(s) 416, 1102
HpyCH4V TGCA 4 cut(s) 368, 374, 643, 954
HpyF10VI GCNNNNNNNGC 3 cut(s) 226, 344, 668
HpyF3I CTNAG 1 cut(s) 468
HpySE526I ACGT 2 cut(s) 416, 1102
Hsp92II CATG 6 cut(s) 31, 187, 311, 317, 382, 718
KroI GCCGGC 1 cut(s) 799
KroNI GCCGGC 1 cut(s) 801
Kzo9I GATC 4 cut(s) 121, 505, 726, 736
LmnI GCTCC 2 cut(s) 24, 668
Lsp1109I GCAGC 2 cut(s) 316, 658
LweI GCATC 2 cut(s) 6, 491
MaeI CTAG 1 cut(s) 648
MaeII ACGT 2 cut(s) 416, 1102
MalI GATC 4 cut(s) 123, 507, 728, 738
MboI GATC 4 cut(s) 121, 505, 726, 736
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 3 cut(s) 505, 726, 736
MhlI GDGCHC 3 cut(s) 360, 773, 786
MluCI AATT 6 cut(s) 214, 222, 278, 369, 775, 807
MnlI CCTC 8 cut(s) 325, 473, 540, 632, 692, 697, 845, 1107
MroNI GCCGGC 1 cut(s) 799
MroXI GAANNNNTTC 1 cut(s) 1113
MseI TTAA 4 cut(s) 384, 420, 1041, 1174
MslI CAYNNNNRTG 2 cut(s) 312, 363
MspI CCGG 9 cut(s) 115, 237, 251, 388, 525, 609, 800, 992, 1057
MspR9I CCNGG 5 cut(s) 563, 610, 633, 992, 1092
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 3 cut(s) 563, 633, 1092
MwoI GCNNNNNNNGC 3 cut(s) 226, 344, 668
NaeI GCCGGC 1 cut(s) 801
NciI CCSGG 2 cut(s) 610, 992
NdeI CATATG 1 cut(s) 694
NdeII GATC 4 cut(s) 121, 505, 726, 736
NgoMIV GCCGGC 1 cut(s) 799
NlaIII CATG 6 cut(s) 31, 187, 311, 317, 382, 718
NlaIV GGNNCC 2 cut(s) 703, 771
NspI RCATGY 3 cut(s) 187, 311, 317
PciI ACATGT 1 cut(s) 313
PdiI GCCGGC 1 cut(s) 801
PdmI GAANNNNTTC 1 cut(s) 1113
PfeI GAWTC 3 cut(s) 262, 283, 443
PfoI TCCNGGA 1 cut(s) 631
PkrI GCNGC 3 cut(s) 306, 673, 943
PpuMI RGGWCCY 1 cut(s) 1094
PscI ACATGT 1 cut(s) 313
Psp1406I AACGTT 2 cut(s) 416, 1102
Psp5II RGGWCCY 1 cut(s) 1094
Psp6I CCWGG 3 cut(s) 561, 631, 1090
PspGI CCWGG 3 cut(s) 561, 631, 1090
PspN4I GGNNCC 2 cut(s) 703, 771
PspOMI GGGCCC 1 cut(s) 769
PspPI GGNCC 4 cut(s) 769, 770, 1088, 1094
PspPPI RGGWCCY 1 cut(s) 1094
PsuI RGATCY 3 cut(s) 505, 726, 736
RigI GGCCGGCC 1 cut(s) 803
RsaI GTAC 4 cut(s) 207, 312, 401, 655
RsaNI GTAC 4 cut(s) 206, 311, 400, 654
RseI CAYNNNNRTG 2 cut(s) 312, 363
SaqAI TTAA 4 cut(s) 384, 420, 1041, 1174
SatI GCNGC 3 cut(s) 305, 672, 942
Sau3AI GATC 4 cut(s) 121, 505, 726, 736
Sau96I GGNCC 4 cut(s) 769, 770, 1088, 1094
ScrFI CCNGG 5 cut(s) 563, 610, 633, 992, 1092
SduI GDGCHC 3 cut(s) 360, 773, 786
SfaNI GCATC 2 cut(s) 6, 491
SinI GGWCC 2 cut(s) 1088, 1094
SmiMI CAYNNNNRTG 2 cut(s) 312, 363
Sse9I AATT 6 cut(s) 214, 222, 278, 369, 775, 807
SsiI CCGC 5 cut(s) 503, 590, 941, 1065, 1086
SspI AATATT 1 cut(s) 1031
SspMI CTAG 1 cut(s) 648
StyD4I CCNGG 5 cut(s) 561, 608, 631, 990, 1090
TaaI ACNGT 4 cut(s) 517, 892, 1039, 1129
TaiI ACGT 2 cut(s) 419, 1105
TaqI TCGA 5 cut(s) 135, 545, 624, 689, 878
TaqII GACCGA 1 cut(s) 531
TasI AATT 6 cut(s) 214, 222, 278, 369, 775, 807
TatI WGTACW 1 cut(s) 310
TauI GCSGC 1 cut(s) 944
TfiI GAWTC 3 cut(s) 262, 283, 443
Tru1I TTAA 4 cut(s) 384, 420, 1041, 1174
Tru9I TTAA 4 cut(s) 384, 420, 1041, 1174
TseI GCWGC 2 cut(s) 304, 671
TspDTI ATGAA 1 cut(s) 28
TspGWI ACGGA 2 cut(s) 134, 417
VpaK11BI GGWCC 2 cut(s) 1088, 1094
XapI RAATTY 2 cut(s) 222, 775
XceI RCATGY 3 cut(s) 187, 311, 317
XmnI GAANNNNTTC 1 cut(s) 1113
XspI CTAG 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.