Rh6BG503700

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0011824
Physical Location & Seq
Reverse (-)
70257441 .. 70259583
2143 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG503700.1

Sequence Viewer

Length: 939 bp
ATGGGGAAACAATTGAGTTGGGGTGGCCTGATCAGATTGAGGGGAATGGTAATTATTCTGGGAGTTGCTCTAATTGGATATTTTGGGGTACTTGGGGAGGGTAACATGTTCTCTAAACAGAAGGTTTTGGAAGTTGAAAGAAAATTAAGCCAACTTAGAAAACCTGCAGTTAAAAGCATTCAGAGTGAAGATGGTGATATCATTGATTGCATTGACATTTACAAGCAGCCAGCTTTTGATCATCCTGCTCTCAAAAACCACACCATCCAGATGGCACCTAGTTATGATCCAACAAAGGAGACAGAGTCGACAATGACCAAATTCGAAAGATTAGAGAGGAAGAGGAACCAGCAATCTTCCATGATGGTGACACAACCATGGAAGAAAAATGGAAGCTGTCCACAAGGAACAATCCCGGTGCGAAGAATCAGAAAAGAGGACCTGCTCCGAGCTAGCTCGGTTGAGGCCTATGGAAGGAAGAAACATAGCTCTTCTCGTCCTCATGTTTCGCATGAGTCTAGTACTAGTGACAGCAACAGAACTGTGAACGTTCTACAAGCAAATCACTCGAAGGCAATATTGCTTACCGAAGGATACAGATATAATGGAGCCAAAGGAGATATCAAAGTTTGGACTCCTAATGTTGATTTTGATGATGATTACACTACTTCTAAAGTTTGCCTCATCAATGGTCCTTATTGGGCATTTGAGAGTGTTGAATCTGGATGGGCGGTGAATCCGAGTGTGTACGGAGACAGGCAAACTCGATTTATGGTGTATTGGACGGTCGATGGTTCAAAGAAAACAGGTTGCTTTGATCTCACTTGTCCTGGATTTGTTCAAACTAGCCATGAGATTGCTCTCGGTGCTGCAATTTACCCAATCTCTGTTCCTTATGGCCTTCCGTACGAAATCATCGTCTATGTCTTCAAGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

312

Amino Acids

35.1

Weight (kDa)

9.11

Isoelectric Point (pI)

33.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 59 - 174 5.9e-41 Neprosin activation peptide
Neprosin PF03080 219 - 311 1.5e-27 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 172, 450
AccB1I GGYRCC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 308
AciI CCGC 1 cut(s) 731
AclI AACGTT 1 cut(s) 549
AclWI GGATC 1 cut(s) 281
AcsI RAATTY 1 cut(s) 320
AfaI GTAC 4 cut(s) 90, 523, 749, 908
AfiI CCNNNNNNNGG 1 cut(s) 474
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 5 cut(s) 137, 719, 798, 842, 931
AhlI ACTAGT 1 cut(s) 524
AjnI CCWGG 1 cut(s) 829
AluBI AGCT 5 cut(s) 233, 396, 452, 456, 489
AluI AGCT 5 cut(s) 233, 396, 452, 456, 489
Alw26I GTCTC 2 cut(s) 293, 747
AlwI GGATC 1 cut(s) 281
AoxI GGCC 3 cut(s) 25, 465, 898
ApeKI GCWGC 2 cut(s) 226, 869
ApoI RAATTY 1 cut(s) 320
ArsI GACNNNNNNTTYG 2 cut(s) 903, 935
AspS9I GGNCC 2 cut(s) 439, 692
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 3 cut(s) 206, 379, 745
AsuII TTCGAA 1 cut(s) 324
AsuNHI GCTAGC 1 cut(s) 452
AvaII GGWCC 2 cut(s) 439, 692
BanI GGYRCC 1 cut(s) 274
BbsI GAAGAC 1 cut(s) 919
BbvI GCAGC 2 cut(s) 238, 856
BccI CCATC 6 cut(s) 185, 265, 272, 358, 720, 785
BcgI CGANNNNNNTGC 2 cut(s) 549, 583
BciT130I CCWGG 1 cut(s) 831
BciVI GTATCC 1 cut(s) 587
BclI TGATCA 2 cut(s) 30, 238
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 2 cut(s) 293, 747
BcuI ACTAGT 1 cut(s) 524
BfaI CTAG 5 cut(s) 279, 453, 519, 525, 846
BfmI CTRYAG 1 cut(s) 165
BfuAI ACCTGC 2 cut(s) 172, 450
BfuI GTATCC 1 cut(s) 587
BisI GCNGC 2 cut(s) 227, 870
BlsI GCNGC 2 cut(s) 228, 871
BmcAI AGTACT 1 cut(s) 523
Bme1390I CCNGG 2 cut(s) 416, 831
Bme18I GGWCC 2 cut(s) 439, 692
BmgT120I GGNCC 2 cut(s) 439, 692
BmiI GGNNCC 3 cut(s) 276, 347, 610
BmrFI CCNGG 2 cut(s) 416, 831
BmtI GCTAGC 1 cut(s) 456
BpiI GAAGAC 1 cut(s) 919
Bpu14I TTCGAA 1 cut(s) 324
BpuMI CCSGG 1 cut(s) 416
BsaJI CCNNGG 1 cut(s) 377
BsaXI ACNNNNNCTCC 2 cut(s) 290, 320
Bsc4I CCNNNNNNNGG 1 cut(s) 474
BseBI CCWGG 1 cut(s) 831
BseDI CCNNGG 1 cut(s) 377
BseGI GGATG 3 cut(s) 241, 264, 731
BseLI CCNNNNNNNGG 1 cut(s) 474
BseXI GCAGC 2 cut(s) 238, 856
Bsh1285I CGRYCG 1 cut(s) 789
BshFI GGCC 3 cut(s) 27, 467, 900
BshNI GGYRCC 1 cut(s) 274
BsiEI CGRYCG 1 cut(s) 789
BsiSI CCGG 1 cut(s) 416
BsiWI CGTACG 1 cut(s) 906
BslI CCNNNNNNNGG 1 cut(s) 474
BsmAI GTCTC 2 cut(s) 293, 747
BsmI GAATGC 1 cut(s) 177
BsnI GGCC 3 cut(s) 27, 467, 900
Bsp119I TTCGAA 1 cut(s) 324
Bsp143I GATC 4 cut(s) 30, 238, 286, 817
Bsp19I CCATGG 1 cut(s) 377
BspACI CCGC 1 cut(s) 731
BspANI GGCC 3 cut(s) 27, 467, 900
BspLI GGNNCC 3 cut(s) 276, 347, 610
BspMAI CTGCAG 1 cut(s) 169
BspMI ACCTGC 2 cut(s) 172, 450
BspOI GCTAGC 1 cut(s) 456
BspPI GGATC 1 cut(s) 281
BspQI GCTCTTC 1 cut(s) 496
BspT104I TTCGAA 1 cut(s) 324
BspT107I GGYRCC 1 cut(s) 274
BssECI CCNNGG 1 cut(s) 377
BssMI GATC 4 cut(s) 30, 238, 286, 817
BssT1I CCWWGG 1 cut(s) 377
Bst2UI CCWGG 1 cut(s) 831
Bst4CI ACNGT 2 cut(s) 544, 787
Bst6I CTCTTC 2 cut(s) 335, 496
BstBI TTCGAA 1 cut(s) 324
BstC8I GCNNGC 2 cut(s) 231, 454
BstDEI CTNAG 1 cut(s) 155
BstDSI CCRYGG 1 cut(s) 377
BstENI CCTNNNNNAGG 1 cut(s) 472
BstF5I GGATG 3 cut(s) 241, 264, 731
BstKTI GATC 4 cut(s) 33, 241, 289, 820
BstMAI GTCTC 2 cut(s) 293, 747
BstMBI GATC 4 cut(s) 30, 238, 286, 817
BstMCI CGRYCG 1 cut(s) 789
BstMWI GCNNNNNNNGC 1 cut(s) 866
BstNI CCWGG 1 cut(s) 831
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 2 cut(s) 414, 829
BstSFI CTRYAG 1 cut(s) 165
BstV1I GCAGC 2 cut(s) 238, 856
BstV2I GAAGAC 1 cut(s) 919
BstXI CCANNNNNNTGG 1 cut(s) 271
BsuI GTATCC 1 cut(s) 587
BsuRI GGCC 3 cut(s) 27, 467, 900
BtgI CCRYGG 1 cut(s) 377
BtsCI GGATG 3 cut(s) 241, 264, 731
BveI ACCTGC 2 cut(s) 172, 450
Cac8I GCNNGC 2 cut(s) 231, 454
Cfr13I GGNCC 2 cut(s) 439, 692
Csp6I GTAC 4 cut(s) 89, 522, 748, 907
CviAII CATG 6 cut(s) 106, 361, 378, 503, 512, 851
CviQI GTAC 4 cut(s) 89, 522, 748, 907
DdeI CTNAG 1 cut(s) 155
DpnI GATC 4 cut(s) 32, 240, 288, 819
DpnII GATC 4 cut(s) 30, 238, 286, 817
Eam1104I CTCTTC 2 cut(s) 335, 496
EarI CTCTTC 2 cut(s) 335, 496
Eco130I CCWWGG 1 cut(s) 377
Eco147I AGGCCT 1 cut(s) 467
Eco32I GATATC 2 cut(s) 199, 622
Eco47I GGWCC 2 cut(s) 439, 692
EcoNI CCTNNNNNAGG 1 cut(s) 472
EcoO109I RGGNCCY 1 cut(s) 439
EcoRII CCWGG 1 cut(s) 829
EcoRV GATATC 2 cut(s) 199, 622
EcoT14I CCWWGG 1 cut(s) 377
ErhI CCWWGG 1 cut(s) 377
FaeI CATG 6 cut(s) 109, 364, 381, 506, 515, 854
FatI CATG 6 cut(s) 105, 360, 377, 502, 511, 850
FbaI TGATCA 2 cut(s) 30, 238
FblI GTMKAC 1 cut(s) 308
Fnu4HI GCNGC 2 cut(s) 227, 870
FokI GGATG 3 cut(s) 228, 251, 738
Fsp4HI GCNGC 2 cut(s) 227, 870
FspBI CTAG 5 cut(s) 279, 453, 519, 525, 846
GluI GCNGC 2 cut(s) 227, 870
HaeIII GGCC 3 cut(s) 27, 467, 900
HapII CCGG 1 cut(s) 416
Hin1II CATG 6 cut(s) 109, 364, 381, 506, 515, 854
HincII GTYRAC 1 cut(s) 309
HindII GTYRAC 1 cut(s) 309
HinfI GANTC 6 cut(s) 305, 426, 515, 634, 719, 736
HpaII CCGG 1 cut(s) 416
HphI GGTGA 3 cut(s) 206, 379, 745
Hpy166II GTNNAC 4 cut(s) 309, 401, 547, 748
Hpy188I TCNGA 6 cut(s) 35, 183, 431, 449, 741, 938
Hpy188III TCNNGA 2 cut(s) 268, 723
Hpy8I GTNNAC 4 cut(s) 309, 401, 547, 748
HpyAV CCTTC 5 cut(s) 115, 468, 565, 584, 911
HpyCH4III ACNGT 2 cut(s) 544, 787
HpyCH4IV ACGT 1 cut(s) 549
HpyCH4V TGCA 3 cut(s) 167, 210, 872
HpyF10VI GCNNNNNNNGC 1 cut(s) 866
HpyF3I CTNAG 1 cut(s) 155
HpySE526I ACGT 1 cut(s) 549
Hsp92II CATG 6 cut(s) 109, 364, 381, 506, 515, 854
Ksp22I TGATCA 2 cut(s) 30, 238
Kzo9I GATC 4 cut(s) 30, 238, 286, 817
LguI GCTCTTC 1 cut(s) 496
LmnI GCTCC 2 cut(s) 450, 608
Lsp1109I GCAGC 2 cut(s) 238, 856
MaeI CTAG 5 cut(s) 279, 453, 519, 525, 846
MaeII ACGT 1 cut(s) 549
MaeIII GTNAC 3 cut(s) 101, 367, 527
MalI GATC 4 cut(s) 32, 240, 288, 819
MboI GATC 4 cut(s) 30, 238, 286, 817
MboII GAAGA 8 cut(s) 200, 348, 352, 394, 435, 483, 490, 919
MfeI CAATTG 1 cut(s) 11
MluCI AATT 6 cut(s) 11, 51, 72, 143, 320, 873
MlyI GAGTC 3 cut(s) 314, 524, 628
MmeI TCCRAC 1 cut(s) 314
MnlI CCTC 8 cut(s) 33, 91, 330, 336, 430, 457, 510, 692
MseI TTAA 2 cut(s) 146, 171
MslI CAYNNNNRTG 3 cut(s) 269, 365, 376
MspI CCGG 1 cut(s) 416
MspR9I CCNGG 2 cut(s) 416, 831
MunI CAATTG 1 cut(s) 11
Mva1269I GAATGC 1 cut(s) 177
MvaI CCWGG 1 cut(s) 831
MwoI GCNNNNNNNGC 1 cut(s) 866
NciI CCSGG 1 cut(s) 416
NcoI CCATGG 1 cut(s) 377
NdeII GATC 4 cut(s) 30, 238, 286, 817
NheI GCTAGC 1 cut(s) 452
NlaIII CATG 6 cut(s) 109, 364, 381, 506, 515, 854
NlaIV GGNNCC 3 cut(s) 276, 347, 610
NmuCI GTSAC 2 cut(s) 367, 527
NspI RCATGY 1 cut(s) 109
NspV TTCGAA 1 cut(s) 324
PceI AGGCCT 1 cut(s) 467
PciI ACATGT 1 cut(s) 105
PciSI GCTCTTC 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 915
PctI GAATGC 1 cut(s) 177
PfeI GAWTC 3 cut(s) 426, 719, 736
Pfl23II CGTACG 1 cut(s) 906
PflFI GACNNNGTC 1 cut(s) 304
PfoI TCCNGGA 1 cut(s) 829
PkrI GCNGC 2 cut(s) 228, 871
PleI GAGTC 3 cut(s) 313, 523, 628
PpsI GAGTC 3 cut(s) 313, 523, 628
PpuMI RGGWCCY 1 cut(s) 439
PscI ACATGT 1 cut(s) 105
Psp1406I AACGTT 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 439
Psp6I CCWGG 1 cut(s) 829
PspGI CCWGG 1 cut(s) 829
PspLI CGTACG 1 cut(s) 906
PspN4I GGNNCC 3 cut(s) 276, 347, 610
PspPI GGNCC 2 cut(s) 439, 692
PspPPI RGGWCCY 1 cut(s) 439
PstI CTGCAG 1 cut(s) 169
PsyI GACNNNGTC 1 cut(s) 304
RsaI GTAC 4 cut(s) 90, 523, 749, 908
RsaNI GTAC 4 cut(s) 89, 522, 748, 907
RseI CAYNNNNRTG 3 cut(s) 269, 365, 376
SalI GTCGAC 1 cut(s) 307
SapI GCTCTTC 1 cut(s) 496
SaqAI TTAA 2 cut(s) 146, 171
SatI GCNGC 2 cut(s) 227, 870
Sau3AI GATC 4 cut(s) 30, 238, 286, 817
Sau96I GGNCC 2 cut(s) 439, 692
ScaI AGTACT 1 cut(s) 523
SchI GAGTC 3 cut(s) 314, 524, 628
ScrFI CCNGG 2 cut(s) 416, 831
SfcI CTRYAG 1 cut(s) 165
SfuI TTCGAA 1 cut(s) 324
SinI GGWCC 2 cut(s) 439, 692
SmiMI CAYNNNNRTG 3 cut(s) 269, 365, 376
SpeI ACTAGT 1 cut(s) 524
Sse9I AATT 6 cut(s) 11, 51, 72, 143, 320, 873
SseBI AGGCCT 1 cut(s) 467
SsiI CCGC 1 cut(s) 731
SspI AATATT 1 cut(s) 579
SspMI CTAG 5 cut(s) 279, 453, 519, 525, 846
StuI AGGCCT 1 cut(s) 467
StyD4I CCNGG 2 cut(s) 414, 829
StyI CCWWGG 1 cut(s) 377
TaaI ACNGT 2 cut(s) 544, 787
TaiI ACGT 1 cut(s) 552
TaqI TCGA 5 cut(s) 308, 324, 569, 766, 789
TasI AATT 6 cut(s) 11, 51, 72, 143, 320, 873
TatI WGTACW 1 cut(s) 521
TfiI GAWTC 3 cut(s) 426, 719, 736
Tru1I TTAA 2 cut(s) 146, 171
Tru9I TTAA 2 cut(s) 146, 171
TseFI GTSAC 2 cut(s) 367, 527
TseI GCWGC 2 cut(s) 226, 869
Tsp45I GTSAC 2 cut(s) 367, 527
TspGWI ACGGA 2 cut(s) 765, 894
Tth111I GACNNNGTC 1 cut(s) 304
VpaK11BI GGWCC 2 cut(s) 439, 692
XagI CCTNNNNNAGG 1 cut(s) 472
XapI RAATTY 1 cut(s) 320
XceI RCATGY 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 308
XspI CTAG 5 cut(s) 279, 453, 519, 525, 846
ZrmI AGTACT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.