Prupe.1G415700_v2.0.a1

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0011824
Physical Location & Seq
Forward (+)
36119194 .. 36121463
2270 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G415700.1

Sequence Viewer

Length: 1314 bp
ATGGGGAAAGGGTTGTGTAGTGGTGAAGTGATTAGATTAAGGGGGATGAAGATGATGGTAATTATGTTGGGAGTGACAGTGACTCTAATTAGATTTGGGGTGCTTGTAGAGGGTGATTTGTTCTCTAAACAAAAGATTTTGGAAGTTGAAAGAAAATTAACCCAACTTAGAAGACCTGCAGTTAAAACCATTCAGAGTGAGGATGGTGATATCATTGATTGCATTGACATTTACAAGCAGCCAGCTTTTGGTCATCCTGCTCTCAGAAACCACACCATCCAGATGGCACCTACATATGATGCAACAAAGGAGACAAAGACAATGGGAAAAATGGACAGATGGAAGAAGAGGAATGAAGAACAATCTCCCACTACAGTGAAGCAGACATGGCACAAAAGTGGAAGCTGTCCTCAGGGAACAATCCCGGTCCGAAGAATCCGGAAAAAGGAGCTTCTGAGAGCCAGCTCGGTTAAGGACCATGGAAGAAAGAAGCACAGTTCTACCCTGTCTCGTCATGTTTCAGAGTTCAGTGACAACAAAACTGTGAACCTTCAACGAGCAAACCACTCGAAGGCAATATTGTTTACAGATGGGTATAGATATAATGGAGCCAAAGGAGACATCAAAGTTTATAACCCTGCTGTTGAGCTTGATGATGAATATAGTACTTCTCAAGTTTGTCTCATAAATGGTGCTTATTATGACTTTGAGAGTGTTGAATCTGGATGGGCGGTAAATCCCAGCGTATACGGGGACAGACAGACTCGATTTTTCGTATACTGGACTGTTGATGGTTCAAAGAAAACTGGTTGCTTTGATCTCACTTGTCCTGGATTTGTTCAGACTAGCCATGAAATTGCTCTTGGTGCAGCCATCTATCCAATCTCTGTTCCTAATGGGCTTCCATATCAAATAATAGTCTACATCTACAAGGATCCGGTAACAAGCAATTGGTGGGTGCAATATGGGGAGAAAATTAACGTCGGTTATTGGCCGCCGGAGCTGTTTGTTGCACTGAGCTACCATGCAACGAGTGCTGAATGGGGAGGTGAAGTTTATAGCTCAAGAGTGGGAACAACTCCTCACACAAAAACAGATATGGGCAGTGGACACTTTGCTGATTCTGTTTGGGGGACTTCAGGCGCCATAAGAAGAATCCGAATACATGAGAATTCACCTGGGCTGAAATTCCCTGATATAGTTACTACTTTGATGGATGAGTTCAACTGTTATAATGTTAGGTATCTGTCAGATTATGTTGAAGATCCTGAGTTCTATTATGGAGGGCCTGGCAGAAATTATATGTGCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

438

Amino Acids

49.44

Weight (kDa)

8.78

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 633, 1233
Acc36I ACCTGC 1 cut(s) 184
AccB1I GGYRCC 2 cut(s) 286, 1142
AccB7I CCANNNNNTGG 1 cut(s) 248
AccI GTMKAC 3 cut(s) 747, 777, 921
AccIII TCCGGA 1 cut(s) 438
AciI CCGC 2 cut(s) 731, 995
AclWI GGATC 3 cut(s) 929, 942, 1259
AcoI YGGCCR 1 cut(s) 992
AcsI RAATTY 2 cut(s) 1171, 1187
AcuI CTGAAG 1 cut(s) 1122
AcyI GRCGYC 1 cut(s) 1143
AfaI GTAC 1 cut(s) 667
AfiI CCNNNNNNNGG 3 cut(s) 248, 445, 571
AgsI TTSAA 6 cut(s) 149, 554, 719, 798, 1225, 1262
AjnI CCWGG 3 cut(s) 829, 1177, 1288
AjuI GAANNNNNNNTTGG 2 cut(s) 846, 878
AleI CACNNNNGTG 2 cut(s) 374, 396
AluBI AGCT 8 cut(s) 245, 405, 451, 465, 649, 1003, 1020, 1062
AluI AGCT 8 cut(s) 245, 405, 451, 465, 649, 1003, 1020, 1062
Alw26I GTCTC 4 cut(s) 305, 513, 612, 686
AlwI GGATC 3 cut(s) 929, 942, 1259
Aor13HI TCCGGA 1 cut(s) 438
AoxI GGCC 2 cut(s) 992, 1286
ApeKI GCWGC 2 cut(s) 238, 869
ApoI RAATTY 2 cut(s) 1171, 1187
AspLEI GCGC 1 cut(s) 1145
AspS9I GGNCC 3 cut(s) 427, 475, 1286
AsuC2I CCSGG 1 cut(s) 425
AsuHPI GGTGA 5 cut(s) 35, 125, 218, 1061, 1167
AvaII GGWCC 2 cut(s) 427, 475
AxyI CCTNAGG 1 cut(s) 411
BaeGI GKGCMC 1 cut(s) 1310
BamHI GGATCC 1 cut(s) 934
BanI GGYRCC 2 cut(s) 286, 1142
BbsI GAAGAC 1 cut(s) 178
BbvI GCAGC 2 cut(s) 250, 881
BcgI CGANNNNNNTGC 2 cut(s) 549, 583
BciT130I CCWGG 3 cut(s) 831, 1179, 1290
BcnI CCSGG 1 cut(s) 425
BcoDI GTCTC 4 cut(s) 305, 513, 612, 686
BfaI CTAG 1 cut(s) 846
BfmI CTRYAG 2 cut(s) 177, 372
BfoI RGCGCY 1 cut(s) 1146
BfuAI ACCTGC 1 cut(s) 184
BisI GCNGC 3 cut(s) 239, 870, 995
BlsI GCNGC 3 cut(s) 240, 871, 996
BmcAI AGTACT 1 cut(s) 667
Bme1390I CCNGG 4 cut(s) 425, 831, 1179, 1290
Bme18I GGWCC 2 cut(s) 427, 475
BmgT120I GGNCC 3 cut(s) 427, 475, 1286
BmiI GGNNCC 4 cut(s) 288, 610, 936, 1144
BmrFI CCNGG 4 cut(s) 425, 831, 1179, 1290
BmsI GCATC 1 cut(s) 289
BpiI GAAGAC 1 cut(s) 178
BpuEI CTTGAG 2 cut(s) 657, 1048
BpuMI CCSGG 1 cut(s) 425
BsaHI GRCGYC 1 cut(s) 1143
BsaJI CCNNGG 2 cut(s) 478, 1178
BsaWI WCCGGW 2 cut(s) 438, 937
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 1038, 1068
Bsc4I CCNNNNNNNGG 3 cut(s) 248, 445, 571
Bse1I ACTGG 2 cut(s) 785, 811
Bse21I CCTNAGG 1 cut(s) 411
BseAI TCCGGA 1 cut(s) 438
BseBI CCWGG 3 cut(s) 831, 1179, 1290
BseDI CCNNGG 2 cut(s) 478, 1178
BseGI GGATG 6 cut(s) 51, 208, 253, 276, 731, 1222
BseLI CCNNNNNNNGG 3 cut(s) 248, 445, 571
BseMII CTCAG 5 cut(s) 277, 425, 446, 1007, 1260
BseNI ACTGG 2 cut(s) 785, 811
BseRI GAGGAG 1 cut(s) 1071
BseSI GKGCMC 1 cut(s) 1310
BseXI GCAGC 2 cut(s) 250, 881
BseYI CCCAGC 1 cut(s) 740
BsgI GTGCAG 1 cut(s) 888
BshFI GGCC 2 cut(s) 994, 1288
BshNI GGYRCC 2 cut(s) 286, 1142
BsiSI CCGG 4 cut(s) 425, 439, 938, 998
BslFI GGGAC 2 cut(s) 767, 1147
BslI CCNNNNNNNGG 3 cut(s) 248, 445, 571
BsmAI GTCTC 4 cut(s) 305, 513, 612, 686
BsmFI GGGAC 2 cut(s) 767, 1147
BsnI GGCC 2 cut(s) 994, 1288
Bsp1286I GDGCHC 1 cut(s) 1310
Bsp13I TCCGGA 1 cut(s) 438
Bsp143I GATC 3 cut(s) 817, 934, 1264
Bsp19I CCATGG 1 cut(s) 478
BspACI CCGC 2 cut(s) 731, 995
BspANI GGCC 2 cut(s) 994, 1288
BspCNI CTCAG 5 cut(s) 276, 424, 447, 1008, 1261
BspEI TCCGGA 1 cut(s) 438
BspLI GGNNCC 4 cut(s) 288, 610, 936, 1144
BspMAI CTGCAG 1 cut(s) 181
BspMI ACCTGC 1 cut(s) 184
BspPI GGATC 3 cut(s) 929, 942, 1259
BspT107I GGYRCC 2 cut(s) 286, 1142
BsrI ACTGG 2 cut(s) 785, 811
BssECI CCNNGG 2 cut(s) 478, 1178
BssMI GATC 3 cut(s) 817, 934, 1264
BssNAI GTATAC 2 cut(s) 748, 778
BssNI GRCGYC 1 cut(s) 1143
BssT1I CCWWGG 1 cut(s) 478
Bst1107I GTATAC 2 cut(s) 748, 778
Bst2UI CCWGG 3 cut(s) 831, 1179, 1290
Bst4CI ACNGT 6 cut(s) 79, 376, 497, 544, 787, 1229
Bst6I CTCTTC 1 cut(s) 341
BstACI GRCGYC 1 cut(s) 1143
BstAPI GCANNNNNTGC 1 cut(s) 1034
BstC8I GCNNGC 2 cut(s) 243, 463
BstDEI CTNAG 6 cut(s) 167, 263, 411, 455, 1016, 1269
BstDSI CCRYGG 1 cut(s) 478
BstF5I GGATG 6 cut(s) 51, 208, 253, 276, 731, 1222
BstH2I RGCGCY 1 cut(s) 1146
BstHHI GCGC 1 cut(s) 1145
BstKTI GATC 3 cut(s) 820, 937, 1267
BstMAI GTCTC 4 cut(s) 305, 513, 612, 686
BstMBI GATC 3 cut(s) 817, 934, 1264
BstMWI GCNNNNNNNGC 4 cut(s) 388, 866, 1000, 1034
BstNI CCWGG 3 cut(s) 831, 1179, 1290
BstSCI CCNGG 4 cut(s) 423, 829, 1177, 1288
BstSFI CTRYAG 2 cut(s) 177, 372
BstSLI GKGCMC 1 cut(s) 1310
BstV1I GCAGC 2 cut(s) 250, 881
BstV2I GAAGAC 1 cut(s) 178
BstX2I RGATCY 2 cut(s) 934, 1264
BstXI CCANNNNNNTGG 1 cut(s) 283
BstYI RGATCY 2 cut(s) 934, 1264
BstZ17I GTATAC 2 cut(s) 748, 778
Bsu36I CCTNAGG 1 cut(s) 411
BsuRI GGCC 2 cut(s) 994, 1288
BtgI CCRYGG 1 cut(s) 478
BtsCI GGATG 6 cut(s) 51, 208, 253, 276, 731, 1222
BtsI GCAGTG 1 cut(s) 1111
BtsIMutI CAGTG 5 cut(s) 84, 381, 535, 1013, 1111
BveI ACCTGC 1 cut(s) 184
Cac8I GCNNGC 2 cut(s) 243, 463
CfoI GCGC 1 cut(s) 1145
Cfr13I GGNCC 3 cut(s) 427, 475, 1286
CpoI CGGWCCG 1 cut(s) 427
Csp6I GTAC 1 cut(s) 666
CspI CGGWCCG 1 cut(s) 427
CviAII CATG 6 cut(s) 387, 479, 515, 851, 1025, 1166
CviQI GTAC 1 cut(s) 666
DdeI CTNAG 6 cut(s) 167, 263, 411, 455, 1016, 1269
DinI GGCGCC 1 cut(s) 1144
DpnI GATC 3 cut(s) 819, 936, 1266
DpnII GATC 3 cut(s) 817, 934, 1264
EaeI YGGCCR 1 cut(s) 992
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
Eco130I CCWWGG 1 cut(s) 478
Eco32I GATATC 1 cut(s) 211
Eco47I GGWCC 2 cut(s) 427, 475
Eco57I CTGAAG 1 cut(s) 1122
Eco81I CCTNAGG 1 cut(s) 411
EcoO109I RGGNCCY 1 cut(s) 1286
EcoRI GAATTC 1 cut(s) 1171
EcoRII CCWGG 3 cut(s) 829, 1177, 1288
EcoRV GATATC 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 478
EgeI GGCGCC 1 cut(s) 1144
EheI GGCGCC 1 cut(s) 1144
ErhI CCWWGG 1 cut(s) 478
FaeI CATG 6 cut(s) 390, 482, 518, 854, 1028, 1169
FaqI GGGAC 2 cut(s) 767, 1147
FatI CATG 6 cut(s) 386, 478, 514, 850, 1024, 1165
FauNDI CATATG 1 cut(s) 295
FblI GTMKAC 3 cut(s) 747, 777, 921
Fnu4HI GCNGC 3 cut(s) 239, 870, 995
FokI GGATG 6 cut(s) 58, 215, 240, 263, 738, 1229
Fsp4HI GCNGC 3 cut(s) 239, 870, 995
FspBI CTAG 1 cut(s) 846
GlaI GCGC 1 cut(s) 1144
GluI GCNGC 3 cut(s) 239, 870, 995
GsaI CCCAGC 1 cut(s) 744
HaeII RGCGCY 1 cut(s) 1146
HaeIII GGCC 2 cut(s) 994, 1288
HapII CCGG 4 cut(s) 425, 439, 938, 998
HhaI GCGC 1 cut(s) 1145
Hin1I GRCGYC 1 cut(s) 1143
Hin1II CATG 6 cut(s) 390, 482, 518, 854, 1028, 1169
Hin6I GCGC 1 cut(s) 1143
HinP1I GCGC 1 cut(s) 1143
HinfI GANTC 6 cut(s) 82, 435, 719, 763, 1121, 1155
HpaII CCGG 4 cut(s) 425, 439, 938, 998
HphI GGTGA 5 cut(s) 35, 125, 218, 1061, 1167
Hpy166II GTNNAC 6 cut(s) 547, 585, 748, 778, 922, 1109
Hpy188I TCNGA 8 cut(s) 195, 266, 431, 456, 523, 843, 1160, 1252
Hpy188III TCNNGA 5 cut(s) 280, 439, 723, 1065, 1268
Hpy8I GTNNAC 6 cut(s) 547, 585, 748, 778, 922, 1109
Hpy99I CGWCG 1 cut(s) 986
HpyAV CCTTC 2 cut(s) 560, 565
HpyCH4III ACNGT 6 cut(s) 79, 376, 497, 544, 787, 1229
HpyCH4IV ACGT 1 cut(s) 981
HpyCH4V TGCA 7 cut(s) 179, 222, 302, 869, 961, 1013, 1028
HpyF10VI GCNNNNNNNGC 4 cut(s) 388, 866, 1000, 1034
HpyF3I CTNAG 6 cut(s) 167, 263, 411, 455, 1016, 1269
HpySE526I ACGT 1 cut(s) 981
Hsp92I GRCGYC 1 cut(s) 1143
Hsp92II CATG 6 cut(s) 390, 482, 518, 854, 1028, 1169
HspAI GCGC 1 cut(s) 1143
KasI GGCGCC 1 cut(s) 1142
Kpn2I TCCGGA 1 cut(s) 438
Kzo9I GATC 3 cut(s) 817, 934, 1264
LmnI GCTCC 3 cut(s) 448, 608, 1000
Lsp1109I GCAGC 2 cut(s) 250, 881
LweI GCATC 1 cut(s) 289
MaeI CTAG 1 cut(s) 846
MaeII ACGT 1 cut(s) 981
MaeIII GTNAC 5 cut(s) 73, 79, 530, 940, 1201
MalI GATC 3 cut(s) 819, 936, 1266
MboI GATC 3 cut(s) 817, 934, 1264
MboII GAAGA 9 cut(s) 61, 183, 355, 358, 368, 444, 495, 1164, 1274
MfeI CAATTG 1 cut(s) 949
MflI RGATCY 2 cut(s) 934, 1264
MhlI GDGCHC 1 cut(s) 1310
MluCI AATT 9 cut(s) 60, 87, 155, 855, 949, 975, 1171, 1187, 1297
Mly113I GGCGCC 1 cut(s) 1143
MlyI GAGTC 2 cut(s) 76, 757
MnlI CCTC 7 cut(s) 103, 193, 342, 420, 1040, 1092, 1277
MroI TCCGGA 1 cut(s) 438
MseI TTAA 6 cut(s) 38, 158, 183, 471, 978, 1312
MslI CAYNNNNRTG 3 cut(s) 281, 374, 396
MspI CCGG 4 cut(s) 425, 439, 938, 998
MspR9I CCNGG 4 cut(s) 425, 831, 1179, 1290
MunI CAATTG 1 cut(s) 949
MvaI CCWGG 3 cut(s) 831, 1179, 1290
MwoI GCNNNNNNNGC 4 cut(s) 388, 866, 1000, 1034
NarI GGCGCC 1 cut(s) 1143
NciI CCSGG 1 cut(s) 425
NcoI CCATGG 1 cut(s) 478
NdeI CATATG 1 cut(s) 295
NdeII GATC 3 cut(s) 817, 934, 1264
NlaIII CATG 6 cut(s) 390, 482, 518, 854, 1028, 1169
NlaIV GGNNCC 4 cut(s) 288, 610, 936, 1144
NmuCI GTSAC 3 cut(s) 73, 79, 530
OliI CACNNNNGTG 2 cut(s) 374, 396
PfeI GAWTC 4 cut(s) 435, 719, 1121, 1155
PflMI CCANNNNNTGG 1 cut(s) 248
PfoI TCCNGGA 1 cut(s) 829
PkrI GCNGC 3 cut(s) 240, 871, 996
PleI GAGTC 2 cut(s) 76, 757
PluTI GGCGCC 1 cut(s) 1146
PpsI GAGTC 2 cut(s) 76, 757
PsiI TTATAA 2 cut(s) 633, 1233
Psp6I CCWGG 3 cut(s) 829, 1177, 1288
PspFI CCCAGC 1 cut(s) 740
PspGI CCWGG 3 cut(s) 829, 1177, 1288
PspN4I GGNNCC 4 cut(s) 288, 610, 936, 1144
PspPI GGNCC 3 cut(s) 427, 475, 1286
PstI CTGCAG 1 cut(s) 181
PsuI RGATCY 2 cut(s) 934, 1264
RsaI GTAC 1 cut(s) 667
RsaNI GTAC 1 cut(s) 666
RseI CAYNNNNRTG 3 cut(s) 281, 374, 396
Rsr2I CGGWCCG 1 cut(s) 427
RsrII CGGWCCG 1 cut(s) 427
SaqAI TTAA 6 cut(s) 38, 158, 183, 471, 978, 1312
SatI GCNGC 3 cut(s) 239, 870, 995
Sau3AI GATC 3 cut(s) 817, 934, 1264
Sau96I GGNCC 3 cut(s) 427, 475, 1286
ScaI AGTACT 1 cut(s) 667
SchI GAGTC 2 cut(s) 76, 757
ScrFI CCNGG 4 cut(s) 425, 831, 1179, 1290
SduI GDGCHC 1 cut(s) 1310
SfaNI GCATC 1 cut(s) 289
SfcI CTRYAG 2 cut(s) 177, 372
SfoI GGCGCC 1 cut(s) 1144
SinI GGWCC 2 cut(s) 427, 475
SmiMI CAYNNNNRTG 3 cut(s) 281, 374, 396
SmlI CTYRAG 2 cut(s) 672, 1063
SmoI CTYRAG 2 cut(s) 672, 1063
Sse9I AATT 9 cut(s) 60, 87, 155, 855, 949, 975, 1171, 1187, 1297
SsiI CCGC 2 cut(s) 731, 995
SspDI GGCGCC 1 cut(s) 1142
SspI AATATT 1 cut(s) 579
SspMI CTAG 1 cut(s) 846
StyD4I CCNGG 4 cut(s) 423, 829, 1177, 1288
StyI CCWWGG 1 cut(s) 478
TaaI ACNGT 6 cut(s) 79, 376, 497, 544, 787, 1229
TaiI ACGT 1 cut(s) 984
TaqI TCGA 2 cut(s) 569, 766
TasI AATT 9 cut(s) 60, 87, 155, 855, 949, 975, 1171, 1187, 1297
TatI WGTACW 1 cut(s) 665
TauI GCSGC 1 cut(s) 997
TfiI GAWTC 4 cut(s) 435, 719, 1121, 1155
Tru1I TTAA 6 cut(s) 38, 158, 183, 471, 978, 1312
Tru9I TTAA 6 cut(s) 38, 158, 183, 471, 978, 1312
TscAI CASTG 5 cut(s) 84, 381, 535, 1020, 1111
TseFI GTSAC 3 cut(s) 73, 79, 530
TseI GCWGC 2 cut(s) 238, 869
Tsp45I GTSAC 3 cut(s) 73, 79, 530
TspDTI ATGAA 4 cut(s) 62, 369, 672, 867
TspRI CASTG 5 cut(s) 84, 381, 535, 1020, 1111
Van91I CCANNNNNTGG 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 427, 475
XapI RAATTY 2 cut(s) 1171, 1187
XmiI GTMKAC 3 cut(s) 747, 777, 921
XspI CTAG 1 cut(s) 846
ZrmI AGTACT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.