RchiOBHm_Chr6g0310411

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011824
Physical Location & Seq
Reverse (-)
67775379 .. 67777739
2361 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27914

Sequence Viewer

Length: 1311 bp
ATGGGGAAACAATTGAGTTGGGGTGGCCTGATCAGATTGAGGGGAATGGTAATTATTCTGGGAGTTGCTCTAATTGGATATTTTGGGGTACTTGGGGAGGTCAACATGTTCTCTAAACAGAAGGTTTTGGAAGTTGAAAGAAAATTAAGCCAACTTAGAAAACCTGCAGTTAAAAGCATTCAGAGTGAAGATGGTGATACCATTGATTGCATTGACATTTACAAGCAGCCAGCTTTTGATCATCCTGCTCTTAAAAACCACACCATCCAGATGGCACCTAGTTATGATCCAACAAAGGAGACACAGTCGACAATGACCAAATTCGAAAGATTAGAGAGGAAGAGGAACCAACAGTCTTCCATGATGGTGACTCAACCATGGAAGAAAAATGGAAGCTGTCCACAAGGAACAATCCCTGTGCGAAGAATCAGAAAAGAGTACCTGCTCCGAGCTAGCTCGGTTGAGGCCTATGGAAGGAAGAAACATAGCTCTTCTCGTCCTCATGTTTCGCATGAGTCTAGTACTAGTGACAGCAACAGAACTGTGAACGTCCAACAAGCAAATCACTCGGCAATATTGCTCACCGAAGGATACAGATACAATGGAGCCAAAGGAGATATCAAAGTTTGGACTCCTAATGTTGATTTTGACGATGACTACACTACTTCTAAAGTTTGCCTCATCAATGGTCCTTATTGGGCATTTGAGAGTGTTGAATCTGGATGGGCGGTGAATCCGAGTGTGTACGGAGACAGGCAAACTCGATTTATGGTGTATTGGACGGTCGACGGTTCAAAGAAAACAGGTTGCTTTGATCTCACTTGTCCTGGATTTGTTCAAACTAGCCATGAGATTGCTCTTGGTGCTGCAATTTACCCAATCTCTGTTCCTTACGGCCTTCCATACGAAATCATCGTCTATGTCTTCAAGGACCCCGTAACAAGCAATTGGTGGGTGCAATACGGGGAGAAAATCAACATCGGATACTGGCCGCCGGAGCTGTTTATGGCGTTGCGTGTCCACGCAGAAGCGGTTGAGTGGGGAGGTGAAGTGTATAGTGCAAGAGTGGGTACTAATCCGCACACAAAAACAGGAATGGGTAATGGAAACTTTCCTGATGGCATTTTTGGGGATTCAGGGGTCATTAAAAGAATCAGAATTCATGACAATTCAGCTGAACTCAAATTCCCGGAATGGGTTGAGACTTATGCGGACGAGTTCAACTGTTATGATGTTGATTATGTGTCCGATTACCTTGAAGATCCTGAGTTCTATTATGGAGGGCCTGGCCATAATCCCAAGTGCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

436

Amino Acids

49.29

Weight (kDa)

6.61

Isoelectric Point (pI)

29.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 59 - 173 1e-38 Neprosin activation peptide
Neprosin PF03080 218 - 429 2.5e-66 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 172, 450
AccB1I GGYRCC 1 cut(s) 274
AccI GTMKAC 2 cut(s) 308, 786
AciI CCGC 5 cut(s) 728, 992, 1031, 1079, 1211
AclWI GGATC 2 cut(s) 281, 1256
AcoI YGGCCR 2 cut(s) 989, 1288
AcsI RAATTY 3 cut(s) 320, 1158, 1184
AfaI GTAC 5 cut(s) 90, 440, 523, 746, 1072
AfiI CCNNNNNNNGG 2 cut(s) 474, 1195
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 7 cut(s) 137, 716, 795, 839, 928, 1222, 1259
AhlI ACTAGT 1 cut(s) 524
AjnI CCWGG 2 cut(s) 826, 1285
AloI GAACNNNNNNTCC 2 cut(s) 1170, 1202
AluBI AGCT 7 cut(s) 233, 396, 452, 456, 489, 1000, 1175
AluI AGCT 7 cut(s) 233, 396, 452, 456, 489, 1000, 1175
Alw26I GTCTC 3 cut(s) 293, 744, 1196
AlwI GGATC 2 cut(s) 281, 1256
AoxI GGCC 6 cut(s) 25, 465, 895, 989, 1283, 1288
ApeKI GCWGC 2 cut(s) 226, 866
ApoI RAATTY 3 cut(s) 320, 1158, 1184
ArsI GACNNNNNNTTYG 2 cut(s) 900, 932
AspS9I GGNCC 3 cut(s) 689, 931, 1283
AsuC2I CCSGG 1 cut(s) 1190
AsuHPI GGTGA 5 cut(s) 206, 379, 574, 742, 1058
AsuII TTCGAA 1 cut(s) 324
AsuNHI GCTAGC 1 cut(s) 452
AvaII GGWCC 2 cut(s) 689, 931
BaeGI GKGCMC 1 cut(s) 1307
BalI TGGCCA 1 cut(s) 1290
BanI GGYRCC 1 cut(s) 274
BbsI GAAGAC 2 cut(s) 348, 916
BbvI GCAGC 2 cut(s) 238, 853
BccI CCATC 6 cut(s) 185, 265, 272, 358, 717, 1112
BceAI ACGGC 1 cut(s) 910
BcgI CGANNNNNNTGC 2 cut(s) 549, 583
BciT130I CCWGG 2 cut(s) 828, 1287
BciVI GTATCC 2 cut(s) 584, 977
BclI TGATCA 2 cut(s) 30, 238
BcnI CCSGG 1 cut(s) 1190
BcoDI GTCTC 3 cut(s) 293, 744, 1196
BcuI ACTAGT 1 cut(s) 524
BfaI CTAG 5 cut(s) 279, 453, 519, 525, 843
BfmI CTRYAG 1 cut(s) 165
BfuAI ACCTGC 2 cut(s) 172, 450
BfuI GTATCC 2 cut(s) 584, 977
BisI GCNGC 3 cut(s) 227, 867, 992
BlsI GCNGC 3 cut(s) 228, 868, 993
BmcAI AGTACT 1 cut(s) 523
Bme1390I CCNGG 3 cut(s) 828, 1190, 1287
Bme18I GGWCC 2 cut(s) 689, 931
BmgT120I GGNCC 3 cut(s) 689, 931, 1283
BmiI GGNNCC 4 cut(s) 276, 347, 607, 933
BmrFI CCNGG 3 cut(s) 828, 1190, 1287
BmtI GCTAGC 1 cut(s) 456
BpiI GAAGAC 2 cut(s) 348, 916
Bpu14I TTCGAA 1 cut(s) 324
BpuMI CCSGG 1 cut(s) 1190
BsaJI CCNNGG 1 cut(s) 377
BsaXI ACNNNNNCTCC 4 cut(s) 290, 320, 1035, 1065
Bsc4I CCNNNNNNNGG 2 cut(s) 474, 1195
Bse1I ACTGG 1 cut(s) 992
BseBI CCWGG 2 cut(s) 828, 1287
BseDI CCNNGG 1 cut(s) 377
BseGI GGATG 3 cut(s) 241, 264, 728
BseLI CCNNNNNNNGG 2 cut(s) 474, 1195
BseMII CTCAG 1 cut(s) 1257
BseNI ACTGG 1 cut(s) 992
BseSI GKGCMC 1 cut(s) 1307
BseXI GCAGC 2 cut(s) 238, 853
Bsh1285I CGRYCG 1 cut(s) 786
BshFI GGCC 6 cut(s) 27, 467, 897, 991, 1285, 1290
BshNI GGYRCC 1 cut(s) 274
BsiEI CGRYCG 1 cut(s) 786
BsiSI CCGG 2 cut(s) 995, 1190
BslI CCNNNNNNNGG 2 cut(s) 474, 1195
BsmAI GTCTC 3 cut(s) 293, 744, 1196
BsmI GAATGC 1 cut(s) 177
BsnI GGCC 6 cut(s) 27, 467, 897, 991, 1285, 1290
Bsp119I TTCGAA 1 cut(s) 324
Bsp1286I GDGCHC 1 cut(s) 1307
Bsp143I GATC 5 cut(s) 30, 238, 286, 814, 1261
Bsp19I CCATGG 1 cut(s) 377
BspACI CCGC 5 cut(s) 728, 992, 1031, 1079, 1211
BspANI GGCC 6 cut(s) 27, 467, 897, 991, 1285, 1290
BspCNI CTCAG 1 cut(s) 1258
BspHI TCATGA 1 cut(s) 1162
BspLI GGNNCC 4 cut(s) 276, 347, 607, 933
BspMAI CTGCAG 1 cut(s) 169
BspMI ACCTGC 2 cut(s) 172, 450
BspOI GCTAGC 1 cut(s) 456
BspPI GGATC 2 cut(s) 281, 1256
BspQI GCTCTTC 1 cut(s) 496
BspT104I TTCGAA 1 cut(s) 324
BspT107I GGYRCC 1 cut(s) 274
BsrI ACTGG 1 cut(s) 992
BssECI CCNNGG 1 cut(s) 377
BssMI GATC 5 cut(s) 30, 238, 286, 814, 1261
BssT1I CCWWGG 1 cut(s) 377
Bst2UI CCWGG 2 cut(s) 828, 1287
Bst4CI ACNGT 6 cut(s) 306, 354, 544, 784, 791, 1226
Bst6I CTCTTC 2 cut(s) 335, 496
BstBI TTCGAA 1 cut(s) 324
BstC8I GCNNGC 2 cut(s) 231, 454
BstDEI CTNAG 2 cut(s) 155, 1266
BstDSI CCRYGG 1 cut(s) 377
BstENI CCTNNNNNAGG 1 cut(s) 472
BstF5I GGATG 3 cut(s) 241, 264, 728
BstKTI GATC 5 cut(s) 33, 241, 289, 817, 1264
BstMAI GTCTC 3 cut(s) 293, 744, 1196
BstMBI GATC 5 cut(s) 30, 238, 286, 814, 1261
BstMCI CGRYCG 1 cut(s) 786
BstMWI GCNNNNNNNGC 2 cut(s) 863, 997
BstNI CCWGG 2 cut(s) 828, 1287
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 3 cut(s) 826, 1188, 1285
BstSFI CTRYAG 1 cut(s) 165
BstSLI GKGCMC 1 cut(s) 1307
BstV1I GCAGC 2 cut(s) 238, 853
BstV2I GAAGAC 2 cut(s) 348, 916
BstX2I RGATCY 1 cut(s) 1261
BstXI CCANNNNNNTGG 1 cut(s) 271
BstYI RGATCY 1 cut(s) 1261
BsuI GTATCC 2 cut(s) 584, 977
BsuRI GGCC 6 cut(s) 27, 467, 897, 991, 1285, 1290
BtgI CCRYGG 1 cut(s) 377
BtsCI GGATG 3 cut(s) 241, 264, 728
BveI ACCTGC 2 cut(s) 172, 450
Cac8I GCNNGC 2 cut(s) 231, 454
CciI TCATGA 1 cut(s) 1162
Cfr13I GGNCC 3 cut(s) 689, 931, 1283
Csp6I GTAC 5 cut(s) 89, 439, 522, 745, 1071
CviAII CATG 7 cut(s) 106, 361, 378, 503, 512, 848, 1163
CviQI GTAC 5 cut(s) 89, 439, 522, 745, 1071
DdeI CTNAG 2 cut(s) 155, 1266
DpnI GATC 5 cut(s) 32, 240, 288, 816, 1263
DpnII GATC 5 cut(s) 30, 238, 286, 814, 1261
EaeI YGGCCR 2 cut(s) 989, 1288
Eam1104I CTCTTC 2 cut(s) 335, 496
EarI CTCTTC 2 cut(s) 335, 496
Eco130I CCWWGG 1 cut(s) 377
Eco147I AGGCCT 1 cut(s) 467
Eco32I GATATC 1 cut(s) 619
Eco47I GGWCC 2 cut(s) 689, 931
EcoNI CCTNNNNNAGG 1 cut(s) 472
EcoO109I RGGNCCY 2 cut(s) 931, 1283
EcoRI GAATTC 1 cut(s) 1158
EcoRII CCWGG 2 cut(s) 826, 1285
EcoRV GATATC 1 cut(s) 619
EcoT14I CCWWGG 1 cut(s) 377
ErhI CCWWGG 1 cut(s) 377
FaeI CATG 7 cut(s) 109, 364, 381, 506, 515, 851, 1166
FatI CATG 7 cut(s) 105, 360, 377, 502, 511, 847, 1162
FbaI TGATCA 2 cut(s) 30, 238
FblI GTMKAC 2 cut(s) 308, 786
Fnu4HI GCNGC 3 cut(s) 227, 867, 992
FokI GGATG 3 cut(s) 228, 251, 735
Fsp4HI GCNGC 3 cut(s) 227, 867, 992
FspBI CTAG 5 cut(s) 279, 453, 519, 525, 843
GluI GCNGC 3 cut(s) 227, 867, 992
HaeIII GGCC 6 cut(s) 27, 467, 897, 991, 1285, 1290
HapII CCGG 2 cut(s) 995, 1190
Hin1II CATG 7 cut(s) 109, 364, 381, 506, 515, 851, 1166
HincII GTYRAC 3 cut(s) 103, 309, 787
HindII GTYRAC 3 cut(s) 103, 309, 787
HinfI GANTC 8 cut(s) 370, 426, 515, 631, 716, 733, 1133, 1152
HpaII CCGG 2 cut(s) 995, 1190
HphI GGTGA 5 cut(s) 206, 379, 574, 742, 1058
Hpy166II GTNNAC 7 cut(s) 103, 309, 401, 547, 745, 787, 1021
Hpy188I TCNGA 8 cut(s) 35, 183, 431, 449, 738, 983, 1157, 1249
Hpy188III TCNNGA 5 cut(s) 268, 720, 1115, 1163, 1265
Hpy8I GTNNAC 7 cut(s) 103, 309, 401, 547, 745, 787, 1021
Hpy99I CGWCG 1 cut(s) 791
HpyAV CCTTC 4 cut(s) 115, 468, 581, 908
HpyCH4III ACNGT 6 cut(s) 306, 354, 544, 784, 791, 1226
HpyCH4IV ACGT 1 cut(s) 549
HpyCH4V TGCA 5 cut(s) 167, 210, 869, 958, 1061
HpyF10VI GCNNNNNNNGC 2 cut(s) 863, 997
HpyF3I CTNAG 2 cut(s) 155, 1266
HpySE526I ACGT 1 cut(s) 549
Hsp92II CATG 7 cut(s) 109, 364, 381, 506, 515, 851, 1166
Ksp22I TGATCA 2 cut(s) 30, 238
Kzo9I GATC 5 cut(s) 30, 238, 286, 814, 1261
LguI GCTCTTC 1 cut(s) 496
LmnI GCTCC 3 cut(s) 450, 605, 997
Lsp1109I GCAGC 2 cut(s) 238, 853
MaeI CTAG 5 cut(s) 279, 453, 519, 525, 843
MaeII ACGT 1 cut(s) 549
MaeIII GTNAC 3 cut(s) 367, 527, 937
MalI GATC 5 cut(s) 32, 240, 288, 816, 1263
MboI GATC 5 cut(s) 30, 238, 286, 814, 1261
MboII GAAGA 9 cut(s) 200, 348, 352, 394, 435, 483, 490, 916, 1271
MfeI CAATTG 2 cut(s) 11, 946
MflI RGATCY 1 cut(s) 1261
MhlI GDGCHC 1 cut(s) 1307
MlsI TGGCCA 1 cut(s) 1290
MluNI TGGCCA 1 cut(s) 1290
MlyI GAGTC 3 cut(s) 364, 524, 625
MmeI TCCRAC 2 cut(s) 314, 577
MnlI CCTC 9 cut(s) 33, 91, 330, 336, 457, 510, 689, 1037, 1274
Mox20I TGGCCA 1 cut(s) 1290
MscI TGGCCA 1 cut(s) 1290
MseI TTAA 5 cut(s) 146, 171, 252, 1146, 1309
MslI CAYNNNNRTG 2 cut(s) 269, 365
Msp20I TGGCCA 1 cut(s) 1290
MspA1I CMGCKG 1 cut(s) 1175
MspI CCGG 2 cut(s) 995, 1190
MspR9I CCNGG 3 cut(s) 828, 1190, 1287
MunI CAATTG 2 cut(s) 11, 946
Mva1269I GAATGC 1 cut(s) 177
MvaI CCWGG 2 cut(s) 828, 1287
MwoI GCNNNNNNNGC 2 cut(s) 863, 997
NciI CCSGG 1 cut(s) 1190
NcoI CCATGG 1 cut(s) 377
NdeII GATC 5 cut(s) 30, 238, 286, 814, 1261
NheI GCTAGC 1 cut(s) 452
NlaIII CATG 7 cut(s) 109, 364, 381, 506, 515, 851, 1166
NlaIV GGNNCC 4 cut(s) 276, 347, 607, 933
NmeAIII GCCGAG 1 cut(s) 548
NmuCI GTSAC 2 cut(s) 367, 527
NspI RCATGY 1 cut(s) 109
NspV TTCGAA 1 cut(s) 324
PagI TCATGA 1 cut(s) 1162
PceI AGGCCT 1 cut(s) 467
PciI ACATGT 1 cut(s) 105
PciSI GCTCTTC 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 912
PctI GAATGC 1 cut(s) 177
PfeI GAWTC 5 cut(s) 426, 716, 733, 1133, 1152
PflFI GACNNNGTC 1 cut(s) 304
PfoI TCCNGGA 2 cut(s) 826, 1188
PkrI GCNGC 3 cut(s) 228, 868, 993
PleI GAGTC 3 cut(s) 364, 523, 625
PpsI GAGTC 3 cut(s) 364, 523, 625
PpuMI RGGWCCY 1 cut(s) 931
PscI ACATGT 1 cut(s) 105
Psp5II RGGWCCY 1 cut(s) 931
Psp6I CCWGG 2 cut(s) 826, 1285
PspGI CCWGG 2 cut(s) 826, 1285
PspN4I GGNNCC 4 cut(s) 276, 347, 607, 933
PspPI GGNCC 3 cut(s) 689, 931, 1283
PspPPI RGGWCCY 1 cut(s) 931
PstI CTGCAG 1 cut(s) 169
PsuI RGATCY 1 cut(s) 1261
PsyI GACNNNGTC 1 cut(s) 304
PvuII CAGCTG 1 cut(s) 1175
RsaI GTAC 5 cut(s) 90, 440, 523, 746, 1072
RsaNI GTAC 5 cut(s) 89, 439, 522, 745, 1071
RseI CAYNNNNRTG 2 cut(s) 269, 365
SalI GTCGAC 2 cut(s) 307, 785
SapI GCTCTTC 1 cut(s) 496
SaqAI TTAA 5 cut(s) 146, 171, 252, 1146, 1309
SatI GCNGC 3 cut(s) 227, 867, 992
Sau3AI GATC 5 cut(s) 30, 238, 286, 814, 1261
Sau96I GGNCC 3 cut(s) 689, 931, 1283
ScaI AGTACT 1 cut(s) 523
SchI GAGTC 3 cut(s) 364, 524, 625
ScrFI CCNGG 3 cut(s) 828, 1190, 1287
SduI GDGCHC 1 cut(s) 1307
SfcI CTRYAG 1 cut(s) 165
SfuI TTCGAA 1 cut(s) 324
SinI GGWCC 2 cut(s) 689, 931
SmiMI CAYNNNNRTG 2 cut(s) 269, 365
SpeI ACTAGT 1 cut(s) 524
SseBI AGGCCT 1 cut(s) 467
SsiI CCGC 5 cut(s) 728, 992, 1031, 1079, 1211
SspI AATATT 1 cut(s) 576
SspMI CTAG 5 cut(s) 279, 453, 519, 525, 843
StuI AGGCCT 1 cut(s) 467
StyD4I CCNGG 3 cut(s) 826, 1188, 1285
StyI CCWWGG 1 cut(s) 377
TaaI ACNGT 6 cut(s) 306, 354, 544, 784, 791, 1226
TaiI ACGT 1 cut(s) 552
TaqI TCGA 4 cut(s) 308, 324, 763, 786
TatI WGTACW 1 cut(s) 521
TauI GCSGC 1 cut(s) 994
TfiI GAWTC 5 cut(s) 426, 716, 733, 1133, 1152
Tru1I TTAA 5 cut(s) 146, 171, 252, 1146, 1309
Tru9I TTAA 5 cut(s) 146, 171, 252, 1146, 1309
TseFI GTSAC 2 cut(s) 367, 527
TseI GCWGC 2 cut(s) 226, 866
Tsp45I GTSAC 2 cut(s) 367, 527
TspDTI ATGAA 1 cut(s) 1151
TspGWI ACGGA 1 cut(s) 762
Tth111I GACNNNGTC 1 cut(s) 304
VpaK11BI GGWCC 2 cut(s) 689, 931
XagI CCTNNNNNAGG 1 cut(s) 472
XapI RAATTY 3 cut(s) 320, 1158, 1184
XceI RCATGY 1 cut(s) 109
XmiI GTMKAC 2 cut(s) 308, 786
XspI CTAG 5 cut(s) 279, 453, 519, 525, 843
ZrmI AGTACT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.