AT5G20165

Involved in the early part of the secretory pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004822
Physical Location & Seq
Reverse (-)
6805542 .. 6807345
1804 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20165.3

Sequence Viewer

Length: 213 bp
ATGTCAGCTTTGTTCAATTTCCATTCGTTTCTGACGGTGGTGCTGCTTGTGATCTGCACATGCACATATCTGAAGATGCAGTTCCCTGCCATTTTAGAGCAGAAAACTGGGTTTCGAGGATTTTTCTGGAAGGCTGCTAGAATAGGTGAGCGTTTGAGCCCTTGGGTCTCCGCTGGATGCTTCATGATGGGTGTGTCGATCATTTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1242

Protein Analysis

70

Amino Acids

8.01

Weight (kDa)

9.42

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1242 PF06842 10 - 38 1.5e-12 Protein of unknown function (DUF1242)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 171
AcuI CTGAAG 1 cut(s) 92
AgsI TTSAA 1 cut(s) 16
AluBI AGCT 1 cut(s) 8
AluI AGCT 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 172
ApeKI GCWGC 2 cut(s) 43, 134
AsuHPI GGTGA 1 cut(s) 158
BanII GRGCYC 1 cut(s) 161
BbvI GCAGC 2 cut(s) 30, 121
BccI CCATC 1 cut(s) 181
BcoDI GTCTC 1 cut(s) 172
BfaI CTAG 1 cut(s) 138
BisI GCNGC 2 cut(s) 44, 135
BlsI GCNGC 2 cut(s) 45, 136
BmrI ACTGGG 1 cut(s) 117
BmsI GCATC 2 cut(s) 66, 167
BmuI ACTGGG 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 172
BsaJI CCNNGG 1 cut(s) 161
Bse1I ACTGG 1 cut(s) 112
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 1 cut(s) 182
BseNI ACTGG 1 cut(s) 112
BseXI GCAGC 2 cut(s) 30, 121
BsgI GTGCAG 1 cut(s) 40
BsmAI GTCTC 1 cut(s) 172
Bso31I GGTCTC 1 cut(s) 172
Bsp1286I GDGCHC 1 cut(s) 161
Bsp143I GATC 2 cut(s) 51, 198
BspACI CCGC 1 cut(s) 171
BspHI TCATGA 1 cut(s) 183
BspTNI GGTCTC 1 cut(s) 172
BsrI ACTGG 1 cut(s) 112
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 2 cut(s) 51, 198
BssT1I CCWWGG 1 cut(s) 161
Bst4CI ACNGT 1 cut(s) 37
BstF5I GGATG 1 cut(s) 182
BstKTI GATC 2 cut(s) 54, 201
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 2 cut(s) 51, 198
BstNSI RCATGY 1 cut(s) 63
BstV1I GCAGC 2 cut(s) 30, 121
BtsCI GGATG 1 cut(s) 182
CciI TCATGA 1 cut(s) 183
CviAII CATG 2 cut(s) 60, 184
CviJI RGCY 3 cut(s) 8, 134, 159
CviKI_1 RGCY 3 cut(s) 8, 134, 159
DpnI GATC 2 cut(s) 53, 200
DpnII GATC 2 cut(s) 51, 198
Eco130I CCWWGG 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 161
Eco31I GGTCTC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 92
EcoT14I CCWWGG 1 cut(s) 161
EcoT38I GRGCYC 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 161
FaeI CATG 2 cut(s) 63, 187
FaiI YATR 3 cut(s) 61, 67, 185
FatI CATG 2 cut(s) 59, 183
Fnu4HI GCNGC 2 cut(s) 44, 135
FokI GGATG 1 cut(s) 189
FriOI GRGCYC 1 cut(s) 161
Fsp4HI GCNGC 2 cut(s) 44, 135
FspBI CTAG 1 cut(s) 138
GluI GCNGC 2 cut(s) 44, 135
Hin1II CATG 2 cut(s) 63, 187
HphI GGTGA 1 cut(s) 158
Hpy188I TCNGA 2 cut(s) 33, 72
Hpy188III TCNNGA 2 cut(s) 127, 184
HpyAV CCTTC 1 cut(s) 124
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4V TGCA 3 cut(s) 57, 63, 79
Hsp92II CATG 2 cut(s) 63, 187
Kzo9I GATC 2 cut(s) 51, 198
LpnPI CCDG 4 cut(s) 93, 99, 112, 159
Lsp1109I GCAGC 2 cut(s) 30, 121
LweI GCATC 2 cut(s) 66, 167
MaeI CTAG 1 cut(s) 138
MalI GATC 2 cut(s) 53, 200
MboI GATC 2 cut(s) 51, 198
MboII GAAGA 1 cut(s) 85
MhlI GDGCHC 1 cut(s) 161
MluCI AATT 1 cut(s) 16
MnlI CCTC 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 173
NdeII GATC 2 cut(s) 51, 198
NlaIII CATG 2 cut(s) 63, 187
NspI RCATGY 1 cut(s) 63
PagI TCATGA 1 cut(s) 183
PkrI GCNGC 2 cut(s) 45, 136
SatI GCNGC 2 cut(s) 44, 135
Sau3AI GATC 2 cut(s) 51, 198
SduI GDGCHC 1 cut(s) 161
SetI ASST 2 cut(s) 10, 148
SfaNI GCATC 2 cut(s) 66, 167
Sse9I AATT 1 cut(s) 16
SsiI CCGC 1 cut(s) 171
SspMI CTAG 1 cut(s) 138
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 1 cut(s) 37
TaqI TCGA 2 cut(s) 115, 197
TasI AATT 1 cut(s) 16
TseI GCWGC 2 cut(s) 43, 134
TspDTI ATGAA 1 cut(s) 172
XceI RCATGY 1 cut(s) 63
XspI CTAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.