RLG00000032528

Yos1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004822
Physical Location & Seq
Reverse (-)
16131415 .. 16132817
1403 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032528

Sequence Viewer

Length: 717 bp
ATGGACCACCAAGAACAACACGACAACGGCGACCCCCGAAAACAAGAGCAGAAGAGGGTTCTGGGAATCAATGGCGATGATCAGGAGAACCAGGAACCCAACGAAGAGGAAATTGTAGGCGACCATGAGAAGCGAGACCCAGAGGAAAGTGATGAAGAGGCGCTTGAAGACTTTGGGGATGGCGGAGAGAGTGTTGGAGAAGGAGACGGGCTTGGAGGTGTAGAGGGAGGCGACGGTGAAGACGACGGCGGCGGTGGAGAGGAGGGAGAAGGCGAAGAGGAAGATGAGGTAGAAGAACTGGAAGAGGAGGAGGAGGGTCCATTTGTGGTGGAGCTGGGCCGGGTCGGTGGAGGGGCCCTGGAGCTGGTTGAGGAGTGGATTGAAACGATGTTAATGCCGATGGCTTTGATCGAGGCTTTTCGGGTTTTGTGGCGATTCTGTGTGGCCTGGCTTATGCTCTTCAACGCATTTGGAATATTGAATGAGGAGAGGTTCCTTGCTCCAAGAGGGTACCCAATGCGACACATTCAATTGTTAGCGGCACTAAAAGAACGAGATTTTAGAGAGAGAAGAGATTGGTGGAGGATTATTAAGGCTGCGCTTATATTAATCTTGAATACCTCCCGTTTCTTGAATTTTCTTCTCATAGGGTTAAACACCATCTGCATTGTCACACTACTCTTGTTCAACTCAACGATTATCAAGTTCAATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yos1

Protein Analysis

239

Amino Acids

26.96

Weight (kDa)

4.21

Isoelectric Point (pI)

53.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yos1 PF08571 143 - 229 3e-11 Yos1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 510
AccB1I GGYRCC 1 cut(s) 510
AciI CCGC 4 cut(s) 183, 249, 252, 539
AcsI RAATTY 1 cut(s) 634
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 1 cut(s) 364
AgsI TTSAA 9 cut(s) 167, 383, 463, 481, 530, 616, 634, 688, 709
AjnI CCWGG 3 cut(s) 90, 357, 446
AluBI AGCT 2 cut(s) 334, 364
AluI AGCT 2 cut(s) 334, 364
Alw26I GTCTC 2 cut(s) 129, 198
AoxI GGCC 3 cut(s) 337, 354, 444
ApaI GGGCCC 1 cut(s) 358
ApeKI GCWGC 1 cut(s) 596
ApoI RAATTY 1 cut(s) 634
AseI ATTAAT 1 cut(s) 608
Asp718I GGTACC 1 cut(s) 510
AspLEI GCGC 2 cut(s) 163, 601
AspS9I GGNCC 5 cut(s) 4, 317, 337, 354, 355
AsuC2I CCSGG 1 cut(s) 341
AsuHPI GGTGA 1 cut(s) 248
AvaII GGWCC 2 cut(s) 4, 317
BaeGI GKGCMC 1 cut(s) 358
BanI GGYRCC 1 cut(s) 510
BanII GRGCYC 1 cut(s) 358
BbsI GAAGAC 2 cut(s) 174, 246
BbvI GCAGC 1 cut(s) 583
BccI CCATC 3 cut(s) 173, 394, 668
BceAI ACGGC 2 cut(s) 43, 262
BcgI CGANNNNNNTGC 2 cut(s) 376, 410
BciT130I CCWGG 3 cut(s) 92, 359, 448
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 1 cut(s) 341
BcoDI GTCTC 2 cut(s) 129, 198
BfoI RGCGCY 1 cut(s) 164
BisI GCNGC 3 cut(s) 250, 540, 597
BlsI GCNGC 3 cut(s) 251, 541, 598
Bme1390I CCNGG 4 cut(s) 92, 341, 359, 448
Bme18I GGWCC 2 cut(s) 4, 317
BmgT120I GGNCC 5 cut(s) 4, 317, 337, 354, 355
BmiI GGNNCC 6 cut(s) 96, 318, 355, 356, 494, 512
BmrFI CCNGG 4 cut(s) 92, 341, 359, 448
BpiI GAAGAC 2 cut(s) 174, 246
BpmI CTGGAG 1 cut(s) 380
BpuMI CCSGG 1 cut(s) 341
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 357
BsaXI ACNNNNNCTCC 2 cut(s) 177, 207
Bsc4I CCNNNNNNNGG 1 cut(s) 364
Bse1I ACTGG 1 cut(s) 303
BseBI CCWGG 3 cut(s) 92, 359, 448
BseDI CCNNGG 1 cut(s) 357
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 1 cut(s) 364
BseNI ACTGG 1 cut(s) 303
BseRI GAGGAG 6 cut(s) 275, 320, 323, 326, 386, 500
BseSI GKGCMC 1 cut(s) 358
BseXI GCAGC 1 cut(s) 583
BseYI CCCAGC 1 cut(s) 334
BshFI GGCC 3 cut(s) 339, 356, 446
BshNI GGYRCC 1 cut(s) 510
BsiSI CCGG 1 cut(s) 340
BslI CCNNNNNNNGG 1 cut(s) 364
BsmAI GTCTC 2 cut(s) 129, 198
BsmBI CGTCTC 1 cut(s) 198
BsnI GGCC 3 cut(s) 339, 356, 446
Bso31I GGTCTC 1 cut(s) 129
Bsp120I GGGCCC 1 cut(s) 354
Bsp1286I GDGCHC 1 cut(s) 358
Bsp143I GATC 2 cut(s) 79, 408
BspACI CCGC 4 cut(s) 183, 249, 252, 539
BspANI GGCC 3 cut(s) 339, 356, 446
BspLI GGNNCC 6 cut(s) 96, 318, 355, 356, 494, 512
BspQI GCTCTTC 1 cut(s) 464
BspT107I GGYRCC 1 cut(s) 510
BspTNI GGTCTC 1 cut(s) 129
BsrI ACTGG 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 357
BssMI GATC 2 cut(s) 79, 408
Bst2UI CCWGG 3 cut(s) 92, 359, 448
Bst4CI ACNGT 1 cut(s) 236
Bst6I CTCTTC 7 cut(s) 47, 99, 150, 270, 297, 464, 565
BstF5I GGATG 1 cut(s) 184
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 2 cut(s) 163, 601
BstKTI GATC 2 cut(s) 82, 411
BstMAI GTCTC 2 cut(s) 129, 198
BstMBI GATC 2 cut(s) 79, 408
BstNI CCWGG 3 cut(s) 92, 359, 448
BstSCI CCNGG 4 cut(s) 90, 339, 357, 446
BstSLI GKGCMC 1 cut(s) 358
BstV1I GCAGC 1 cut(s) 583
BstV2I GAAGAC 2 cut(s) 174, 246
BsuRI GGCC 3 cut(s) 339, 356, 446
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 1 cut(s) 184
CfoI GCGC 2 cut(s) 163, 601
Cfr13I GGNCC 5 cut(s) 4, 317, 337, 354, 355
Csp6I GTAC 1 cut(s) 511
CviAII CATG 1 cut(s) 125
CviQI GTAC 1 cut(s) 511
DpnI GATC 2 cut(s) 81, 410
DpnII GATC 2 cut(s) 79, 408
Eam1104I CTCTTC 7 cut(s) 47, 99, 150, 270, 297, 464, 565
EarI CTCTTC 7 cut(s) 47, 99, 150, 270, 297, 464, 565
EciI GGCGGA 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 358
Eco31I GGTCTC 1 cut(s) 129
Eco47I GGWCC 2 cut(s) 4, 317
EcoO109I RGGNCCY 2 cut(s) 354, 355
EcoRII CCWGG 3 cut(s) 90, 357, 446
EcoT38I GRGCYC 1 cut(s) 358
Esp3I CGTCTC 1 cut(s) 198
FaeI CATG 1 cut(s) 128
FaiI YATR 4 cut(s) 126, 455, 605, 647
FalI AAGNNNNNCTT 2 cut(s) 147, 179
FatI CATG 1 cut(s) 124
FbaI TGATCA 1 cut(s) 79
Fnu4HI GCNGC 3 cut(s) 250, 540, 597
FokI GGATG 1 cut(s) 191
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 3 cut(s) 250, 540, 597
GlaI GCGC 2 cut(s) 162, 600
GluI GCNGC 3 cut(s) 250, 540, 597
GsaI CCCAGC 1 cut(s) 338
GsuI CTGGAG 1 cut(s) 380
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 3 cut(s) 339, 356, 446
HapII CCGG 1 cut(s) 340
HhaI GCGC 2 cut(s) 163, 601
Hin1II CATG 1 cut(s) 128
Hin6I GCGC 2 cut(s) 161, 599
HinP1I GCGC 2 cut(s) 161, 599
HinfI GANTC 2 cut(s) 66, 435
HpaII CCGG 1 cut(s) 340
HphI GGTGA 1 cut(s) 248
Hpy188III TCNNGA 3 cut(s) 83, 613, 631
Hpy99I CGWCG 2 cut(s) 236, 248
HpyAV CCTTC 2 cut(s) 194, 263
HpyCH4III ACNGT 1 cut(s) 236
HpyCH4V TGCA 1 cut(s) 666
Hsp92II CATG 1 cut(s) 128
HspAI GCGC 2 cut(s) 161, 599
KpnI GGTACC 1 cut(s) 514
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 2 cut(s) 79, 408
LguI GCTCTTC 1 cut(s) 464
LmnI GCTCC 3 cut(s) 331, 361, 505
Lsp1109I GCAGC 1 cut(s) 583
MaeIII GTNAC 1 cut(s) 670
MalI GATC 2 cut(s) 81, 410
MboI GATC 2 cut(s) 79, 408
MfeI CAATTG 1 cut(s) 530
MhlI GDGCHC 1 cut(s) 358
MluCI AATT 4 cut(s) 111, 530, 634, 709
MmeI TCCRAC 1 cut(s) 175
MseI TTAA 4 cut(s) 392, 591, 608, 653
MspI CCGG 1 cut(s) 340
MspR9I CCNGG 4 cut(s) 92, 341, 359, 448
MunI CAATTG 1 cut(s) 530
MvaI CCWGG 3 cut(s) 92, 359, 448
NciI CCSGG 1 cut(s) 341
NdeII GATC 2 cut(s) 79, 408
NlaIII CATG 1 cut(s) 128
NlaIV GGNNCC 6 cut(s) 96, 318, 355, 356, 494, 512
NmuCI GTSAC 1 cut(s) 670
PciSI GCTCTTC 1 cut(s) 464
PcsI WCGNNNNNNNCGW 2 cut(s) 27, 240
PfeI GAWTC 2 cut(s) 66, 435
PkrI GCNGC 3 cut(s) 251, 541, 598
PshBI ATTAAT 1 cut(s) 608
Psp6I CCWGG 3 cut(s) 90, 357, 446
PspFI CCCAGC 1 cut(s) 334
PspGI CCWGG 3 cut(s) 90, 357, 446
PspN4I GGNNCC 6 cut(s) 96, 318, 355, 356, 494, 512
PspOMI GGGCCC 1 cut(s) 354
PspPI GGNCC 5 cut(s) 4, 317, 337, 354, 355
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
SapI GCTCTTC 1 cut(s) 464
SaqAI TTAA 4 cut(s) 392, 591, 608, 653
SatI GCNGC 3 cut(s) 250, 540, 597
Sau3AI GATC 2 cut(s) 79, 408
Sau96I GGNCC 5 cut(s) 4, 317, 337, 354, 355
ScrFI CCNGG 4 cut(s) 92, 341, 359, 448
SduI GDGCHC 1 cut(s) 358
SetI ASST 6 cut(s) 220, 291, 336, 366, 494, 623
SinI GGWCC 2 cut(s) 4, 317
Sse9I AATT 4 cut(s) 111, 530, 634, 709
SsiI CCGC 4 cut(s) 183, 249, 252, 539
SspI AATATT 1 cut(s) 477
StyD4I CCNGG 4 cut(s) 90, 339, 357, 446
TaaI ACNGT 1 cut(s) 236
TaqI TCGA 1 cut(s) 411
TasI AATT 4 cut(s) 111, 530, 634, 709
TauI GCSGC 2 cut(s) 252, 542
TfiI GAWTC 2 cut(s) 66, 435
Tru1I TTAA 4 cut(s) 392, 591, 608, 653
Tru9I TTAA 4 cut(s) 392, 591, 608, 653
TseFI GTSAC 1 cut(s) 670
TseI GCWGC 1 cut(s) 596
Tsp45I GTSAC 1 cut(s) 670
TspDTI ATGAA 1 cut(s) 168
VpaK11BI GGWCC 2 cut(s) 4, 317
VspI ATTAAT 1 cut(s) 608
XapI RAATTY 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.