MD14G1133000.v1.1

Involved in the early part of the secretory pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004822
Physical Location & Seq
Forward (+)
21083665 .. 21085252
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1133000.v1.1.491

Sequence Viewer

Length: 213 bp
ATGTCGGCGCTTTTCAATTTCAATTCGTTTTTGACGGTGGTGCTGCTCGTGATTTGCACGTGCACGTTTGTGAAGATGCAGTTTCCGGCCGTCCTGGAGCAGAAAACAGGGTTCCGGGGATTTTTCTGGAAGGCAGCTAGAATAGGCGAACGCTTGAGCCCTTGGGTTGCGTTTGGATGCTTTACAATGGGGATCTCAATTATATTTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1242

Protein Analysis

71

Amino Acids

7.98

Weight (kDa)

9.5

Isoelectric Point (pI)

35.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1242 PF06842 10 - 43 1.4e-16 Protein of unknown function (DUF1242)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 200
AcoI YGGCCR 1 cut(s) 87
AcvI CACGTG 1 cut(s) 60
AgsI TTSAA 2 cut(s) 16, 22
AjnI CCWGG 1 cut(s) 93
AleI CACNNNNGTG 1 cut(s) 68
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
Alw21I GWGCWC 1 cut(s) 65
Alw44I GTGCAC 1 cut(s) 61
AlwI GGATC 1 cut(s) 200
AoxI GGCC 1 cut(s) 87
ApaLI GTGCAC 1 cut(s) 61
ApeKI GCWGC 2 cut(s) 43, 134
AspLEI GCGC 1 cut(s) 10
AsuC2I CCSGG 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 161
BauI CACGAG 1 cut(s) 47
BbrPI CACGTG 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 30, 146
BceAI ACGGC 1 cut(s) 74
BciT130I CCWGG 1 cut(s) 95
BcnI CCSGG 1 cut(s) 116
BfaI CTAG 2 cut(s) 138, 211
BfoI RGCGCY 1 cut(s) 11
BisI GCNGC 2 cut(s) 44, 135
BlsI GCNGC 2 cut(s) 45, 136
Bme1390I CCNGG 2 cut(s) 95, 116
BmiI GGNNCC 1 cut(s) 113
BmrFI CCNGG 2 cut(s) 95, 116
BmsI GCATC 2 cut(s) 66, 167
BpmI CTGGAG 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 175
BpuMI CCSGG 1 cut(s) 116
BsaAI YACGTR 1 cut(s) 60
BsaJI CCNNGG 2 cut(s) 115, 161
BseBI CCWGG 1 cut(s) 95
BseDI CCNNGG 2 cut(s) 115, 161
BseGI GGATG 1 cut(s) 182
BseSI GKGCMC 1 cut(s) 65
BseX3I CGGCCG 1 cut(s) 87
BseXI GCAGC 2 cut(s) 30, 146
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 2 cut(s) 86, 115
BsnI GGCC 1 cut(s) 89
Bsp1286I GDGCHC 2 cut(s) 65, 161
Bsp143I GATC 1 cut(s) 192
BspANI GGCC 1 cut(s) 89
BspLI GGNNCC 1 cut(s) 113
BspPI GGATC 1 cut(s) 200
BssECI CCNNGG 2 cut(s) 115, 161
BssMI GATC 1 cut(s) 192
BssSI CACGAG 1 cut(s) 47
BssT1I CCWWGG 1 cut(s) 161
Bst2BI CACGAG 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 37
BstBAI YACGTR 1 cut(s) 60
BstF5I GGATG 1 cut(s) 182
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 1 cut(s) 195
BstMBI GATC 1 cut(s) 192
BstMCI CGRYCG 1 cut(s) 90
BstNI CCWGG 1 cut(s) 95
BstSCI CCNGG 2 cut(s) 93, 114
BstSLI GKGCMC 1 cut(s) 65
BstV1I GCAGC 2 cut(s) 30, 146
BstX2I RGATCY 1 cut(s) 192
BstYI RGATCY 1 cut(s) 192
BstZI CGGCCG 1 cut(s) 87
BsuRI GGCC 1 cut(s) 89
BtsCI GGATG 1 cut(s) 182
CfoI GCGC 1 cut(s) 10
CviJI RGCY 3 cut(s) 89, 137, 159
CviKI_1 RGCY 3 cut(s) 89, 137, 159
DpnI GATC 1 cut(s) 194
DpnII GATC 1 cut(s) 192
EaeI YGGCCR 1 cut(s) 87
EagI CGGCCG 1 cut(s) 87
EclXI CGGCCG 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 161
Eco52I CGGCCG 1 cut(s) 87
Eco72I CACGTG 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 93
EcoT14I CCWWGG 1 cut(s) 161
EcoT38I GRGCYC 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 161
FaiI YATR 1 cut(s) 203
Fnu4HI GCNGC 2 cut(s) 44, 135
FokI GGATG 1 cut(s) 189
FriOI GRGCYC 1 cut(s) 161
Fsp4HI GCNGC 2 cut(s) 44, 135
FspBI CTAG 2 cut(s) 138, 211
GlaI GCGC 1 cut(s) 9
GluI GCNGC 2 cut(s) 44, 135
GsuI CTGGAG 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 1 cut(s) 89
HapII CCGG 2 cut(s) 86, 115
HhaI GCGC 1 cut(s) 10
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HpaII CCGG 2 cut(s) 86, 115
Hpy166II GTNNAC 1 cut(s) 63
Hpy188III TCNNGA 2 cut(s) 49, 127
Hpy8I GTNNAC 1 cut(s) 63
HpyAV CCTTC 1 cut(s) 124
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 2 cut(s) 59, 65
HpyCH4V TGCA 3 cut(s) 57, 63, 79
HpySE526I ACGT 2 cut(s) 59, 65
HspAI GCGC 1 cut(s) 8
Kzo9I GATC 1 cut(s) 192
LmnI GCTCC 1 cut(s) 97
LpnPI CCDG 6 cut(s) 80, 93, 99, 107, 112, 128
Lsp1109I GCAGC 2 cut(s) 30, 146
LweI GCATC 2 cut(s) 66, 167
MaeI CTAG 2 cut(s) 138, 211
MaeII ACGT 2 cut(s) 59, 65
MalI GATC 1 cut(s) 194
MboI GATC 1 cut(s) 192
MboII GAAGA 1 cut(s) 85
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 2 cut(s) 65, 161
MluCI AATT 3 cut(s) 16, 22, 198
MslI CAYNNNNRTG 1 cut(s) 68
MspI CCGG 2 cut(s) 86, 115
MspR9I CCNGG 2 cut(s) 95, 116
MvaI CCWGG 1 cut(s) 95
NciI CCSGG 1 cut(s) 116
NdeII GATC 1 cut(s) 192
NlaIV GGNNCC 1 cut(s) 113
OliI CACNNNNGTG 1 cut(s) 68
PfoI TCCNGGA 1 cut(s) 93
PkrI GCNGC 2 cut(s) 45, 136
PmaCI CACGTG 1 cut(s) 60
PmlI CACGTG 1 cut(s) 60
Ppu21I YACGTR 1 cut(s) 60
Psp6I CCWGG 1 cut(s) 93
PspCI CACGTG 1 cut(s) 60
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 113
PsuI RGATCY 1 cut(s) 192
RseI CAYNNNNRTG 1 cut(s) 68
SatI GCNGC 2 cut(s) 44, 135
Sau3AI GATC 1 cut(s) 192
ScrFI CCNGG 2 cut(s) 95, 116
SduI GDGCHC 2 cut(s) 65, 161
SetI ASST 3 cut(s) 62, 68, 139
SfaNI GCATC 2 cut(s) 66, 167
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
Sse9I AATT 3 cut(s) 16, 22, 198
SspMI CTAG 2 cut(s) 138, 211
StyD4I CCNGG 2 cut(s) 93, 114
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 2 cut(s) 62, 68
TasI AATT 3 cut(s) 16, 22, 198
TseI GCWGC 2 cut(s) 43, 134
VneI GTGCAC 1 cut(s) 61
XspI CTAG 2 cut(s) 138, 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.