Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005278

Physical Location & Seq

Genomic Coordinates Reverse (-)

7904293 .. 7909623

Gene Structure 5331 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G23450.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2292 bp

ATGCAGAAGAGTGGTGTTAATCGGAACCCTTCTTTGAAAGTAGCCATTCCTCAGGCACAACAGTCGCTTAGACGTTTAGGTTTTTGCTCCCAAATAGCTACGGGTGGATCTCAGCAGTCATCTCCTATAGTATTCCCTGAAAAGAGAAATAAGAAGGTGAAGGCATCTTCGAGACGTGGTGAGGTTACTAATGATCCACAGGTGAAACCGAAGCCTGATGAGCATCGGATCGATATTGGTGGAGGGGATGAGAAATCAGATTTGTTAGGTAGTCTTGTTTACGCTGGGAAGCTTGTGTTGGATAAGAGGAAATCTGCGAGTGGAAAAGATGCAACAGAGATACAGCAACCAGCCGCTACGGACATCTCTATTAAGAAAGCAGTTGATGCAAAGCTTACAAGCAGCGCTTTGGTTTGGGGTTCTGACATGCTACAGCTTAACGATGTTGTCTCGGTGACATACAATGTTGGTCTTAGGCATTTCACTGTGCATGCTTATCCAATTGGCAAGGGTTCTTGTGGCCTGTCTTGCTTCACGAAACCAAAGAGAAGCCGCAAGGATTTCCGCTTTGTCGCACCTACAGTAGAAGAAGCCGTTCAATGGGTGGCCAGTTTTGGAGATCAACAATGTTTTATCAACTGTTTGCCACATCCTTTAGTCGCAAAGAAACAGGCTTCGTCTGAGTTATTTTCGGTCCCCATTGATACCCCTCCTGAGTTGGTCTTCAGGTGTAAGAGTGCACCCAAAATGCTTGTCATATTGAACCCTAGGTCAGGACATGGACGATCTATCAAAGTTTTCCATAATGTCGTGGAACCAATTTTCAAGCTGGCCGGAATTAAGATGGAGGTTGTCAAAACAACTAAAGCAGGTCATGCGAGAGAATTGGCTTCCACTGTCGACATTAACTTATGCTCTGACGGAATTATTTGTGTTGGAGGGGATGGAATCATCAATGAGGTTCTTAATGGTCTGCTTACTCGAAGCAATCCGAAAGAAGGAGTTTCTATCCCAATTGGAATAGTACCTGCTGGTTCTGATAACTCGTTAGTTTGGACTGTCCTTGGTGTAAGAGATCCTATTTCTGCAGCACTCTCTATTGTGAAGGGTGGCCTAACAGCTACTGATGTCTTTGCTGTTGAATGGATTCATACCGGTATTATACACTTTGGAATGACTGTTTCCTACTATGGTTTTGTTAGCGATGTTTTGGAGCTCTCTGAAAAATACCAAAAACGGTTTGGTCCTTTGCGTTACTTTGTTGCTGGATTCCTCAAGTTCATGTGTTTGCCGAAGTATAGCTATGAAGTGGAATATCTTCCGGCACAAAAAGAGGATGCAGAAGGCAAAATTCGACTTGAAAAGGAAGCTGTGGATATGCAAGATCTCTACACGGATGTAATGAGGAGATCAAGCAGAGAAGGATTTCCTAGAGCCTCCAGTTTATCAAGTATTGACTCCATAATGACCCCAAGCGTAGGCGAATTAGACACTTGTAGCAGCACACATGCCAGCACTGAGCCGTCTGAATATGTCCGTGGAATAGATCCTAAAATGAAACGCTTGTCCTCTGGAAGAAGAGATGTTACGGCTGAGCCAGAGGTTATTCATCCTCAAGCACAGTCAACAACCCCTAATTGGCCAAGGACAAGATCAAAGTCAAGAATGGATAAAGGATGGATGGGGTTAACATCCGTGCAGGATCCTCCAACTCGATGTTCATGGGGGAATACCGGTGGTCAAGACAGGGAAGATATATCATCTACTGTATCTGATCCAGGTCCAATCTGGGATGCAGGACCCAAATGGGACACTGAACCGTCTGCTTGGGATGTAGAAAATTCGATCGAGCTGCCTGGTCCTCCTGAGGATATAGAGACAGGACTGAGGAAACAGAGCATCACTCCAATATTTGAGGATAAATGGGTTTCGAGAAAGGGACATTTCCTCGGCATCATGGTCTGTAACCATGCTTGTCGGACTGTGCAAAGTTCCCAAGTCGTGGCTCCCAATTCAGAACATGATGATGGTACAATGGATATGCTCTTGGTTCATGGGTGTGGAAGACTGAGGTTACTGAGGTTTTTTATACTTTTGCAAACAGGTCGACACCTTTCTCTTCCGTATGTTGAGTGTGTAAAGGTGAAATCAGTGAAGATAAAGGCGGGGAAAAATACGCATGACAGTTGCGGGATAGACGGGGAACTGTTTGCATTGCATGGAGAAGTGATATCGACAATGCTACCTGAACAATGCAGACTGATCGGTAACGCTCCTGGACGACATTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006629 GO:0006643 GO:0006665 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0017050 GO:0030148 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0046467 GO:0071704 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAGK_cat

Protein Analysis

763

Amino Acids

83.59

Weight (kDa)

8.65

Isoelectric Point (pI)

41.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	249 - 379	3e-29	Diacylglycerol kinase catalytic domain
CERK_C	PF19280	633 - 725	7.1e-07	Ceramide kinase C-terminal domain
YegS_C	PF19279	663 - 754	5.6e-11	YegS C-terminal NAD kinase beta sandwich-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005278)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23450 AT5G23450 AT5G23450 AT5G23450 AT5G23450
fragaria_vesca	FvH4_4g37380 FvH4_4g37380 FvH4_4g37380
malus_domestica	MD13G1000100.v1.1 MD16G1005700.v1.1 MD16G1005800.v1.1
prunus_persica	Prupe.1G584800_v2.0.a1
pyrus_communis	pycom13g00010 pycom2749g00100 pycom2749g00120
rosa_chinensis	RchiOBHm_Chr4g0447291
rosa_laevigata	RLG00000005589
rosa_multiflora	Rmu_co8061930.1_g000001 Rmu_sc0017601.1_g000001 Rmu_ssc0000396.1_g000006
rosa_roxburghii	Rroxscaffold_5G00387670
rosa_rugosa	Rorug04G0379600 Rorug04G0379600 Rorug04G0379700
rosa_samantha	Rh4AG440900 Rh4BG450000 Rh4CG467100 Rh4DG448400
rosa_wichuraiana	Rw4G037730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	860, 1036
AccB7I	CCANNNNNTGG	1 cut(s)	2002
AccI	GTMKAC	2 cut(s)	900, 2107
AciI	CCGC	5 cut(s)	354, 553, 565, 2165, 2190
AclWI	GGATC	8 cut(s)	115, 188, 236, 1070, 1541, 1697, 1710, 1769
AcoI	YGGCCR	3 cut(s)	606, 831, 1640
AcsI	RAATTY	2 cut(s)	1350, 1840
AcuI	CTGAAG	1 cut(s)	709
AfaI	GTAC	2 cut(s)	1026, 2032
AfeI	AGCGCT	1 cut(s)	406
AfiI	CCNNNNNNNGG	6 cut(s)	600, 773, 998, 1478, 1638, 2002
AgeI	ACCGGT	2 cut(s)	1154, 1733
AgsI	TTSAA	6 cut(s)	37, 599, 763, 826, 1142, 1361
AjiI	CACGTC	1 cut(s)	176
AjnI	CCWGG	3 cut(s)	1777, 1855, 2275
AjuI	GAANNNNNNNTTGG	4 cut(s)	281, 313, 1702, 1734
Alw21I	GWGCWC	2 cut(s)	742, 1218
Alw26I	GTCTC	3 cut(s)	166, 454, 1871
Alw44I	GTGCAC	1 cut(s)	738
AlwI	GGATC	8 cut(s)	115, 188, 236, 1070, 1541, 1697, 1710, 1769
AlwNI	CAGNNNCTG	1 cut(s)	1124
Aor51HI	AGCGCT	1 cut(s)	406
AoxI	GGCC	5 cut(s)	520, 606, 831, 1111, 1640
ApaLI	GTGCAC	1 cut(s)	738
ApeKI	GCWGC	4 cut(s)	402, 1088, 1500, 1852
ApoI	RAATTY	2 cut(s)	1350, 1840
ArsI	GACNNNNNNTTYG	2 cut(s)	738, 770
AsiGI	ACCGGT	2 cut(s)	1154, 1733
Asp700I	GAANNNNTTC	4 cut(s)	594, 1146, 1317, 1425
AspA2I	CCTAGG	1 cut(s)	767
AspLEI	GCGC	1 cut(s)	407
AspS9I	GGNCC	5 cut(s)	694, 1244, 1781, 1799, 1859
AsuHPI	GGTGA	5 cut(s)	169, 191, 214, 466, 2155
AvaII	GGWCC	5 cut(s)	694, 1244, 1781, 1799, 1859
AvrII	CCTAGG	1 cut(s)	767
AxyI	CCTNAGG	2 cut(s)	51, 1866
BaeGI	GKGCMC	1 cut(s)	742
BalI	TGGCCA	2 cut(s)	608, 1642
BamHI	GGATCC	1 cut(s)	1702
BanII	GRGCYC	1 cut(s)	1218
BarI	GAAGNNNNNNTAC	2 cut(s)	1570, 1602
BbsI	GAAGAC	2 cut(s)	715, 2071
Bbv12I	GWGCWC	2 cut(s)	742, 1218
BbvI	GCAGC	4 cut(s)	414, 1100, 1512, 1839
BccI	CCATC	5 cut(s)	838, 938, 1671, 1675, 2021
BceAI	ACGGC	3 cut(s)	578, 1507, 1605
BcgI	CGANNNNNNTGC	8 cut(s)	45, 79, 1834, 1868, 2087, 2121, 2245, 2279
BciT130I	CCWGG	3 cut(s)	1779, 1857, 2277
BcoDI	GTCTC	3 cut(s)	166, 454, 1871
BfaI	CTAG	2 cut(s)	768, 1431
BfmI	CTRYAG	4 cut(s)	126, 431, 579, 1086
BfoI	RGCGCY	1 cut(s)	408
BfuAI	ACCTGC	2 cut(s)	860, 1036
BglII	AGATCT	1 cut(s)	1384
BisI	GCNGC	6 cut(s)	354, 403, 553, 1089, 1501, 1853
BlnI	CCTAGG	1 cut(s)	767
BlpI	GCTNAGC	1 cut(s)	1593
BlsI	GCNGC	6 cut(s)	355, 404, 554, 1090, 1502, 1854
Bme1390I	CCNGG	3 cut(s)	1779, 1857, 2277
Bme18I	GGWCC	5 cut(s)	694, 1244, 1781, 1799, 1859
BmgBI	CACGTC	1 cut(s)	176
BmgT120I	GGNCC	5 cut(s)	694, 1244, 1781, 1799, 1859
BmiI	GGNNCC	6 cut(s)	26, 696, 816, 1704, 1801, 2007
BmrFI	CCNGG	3 cut(s)	1779, 1857, 2277
BmsI	GCATC	8 cut(s)	173, 232, 319, 376, 1327, 1783, 1908, 1962
BpiI	GAAGAC	2 cut(s)	715, 2071
BplI	GAGNNNNNCTC	2 cut(s)	1888, 1920
BpmI	CTGGAG	1 cut(s)	1423
Bpu1102I	GCTNAGC	1 cut(s)	1593
BpuEI	CTTGAG	2 cut(s)	1259, 1599
Bsa29I	ATCGAT	1 cut(s)	231
BsaBI	GATNNNNATC	1 cut(s)	222
BsaJI	CCNNGG	5 cut(s)	767, 1063, 1537, 1643, 1948
BsaWI	WCCGGW	2 cut(s)	1154, 1733
Bsc4I	CCNNNNNNNGG	6 cut(s)	600, 773, 998, 1478, 1638, 2002
Bse118I	RCCGGY	2 cut(s)	1154, 1733
Bse1I	ACTGG	2 cut(s)	609, 1440
Bse21I	CCTNAGG	2 cut(s)	51, 1866
Bse3DI	GCAATG	1 cut(s)	2213
Bse8I	GATNNNNATC	1 cut(s)	222
BseBI	CCWGG	3 cut(s)	1779, 1857, 2277
BseCI	ATCGAT	1 cut(s)	231
BseDI	CCNNGG	5 cut(s)	767, 1063, 1537, 1643, 1948
BseJI	GATNNNNATC	1 cut(s)	222
BseLI	CCNNNNNNNGG	6 cut(s)	600, 773, 998, 1478, 1638, 2002
BseMI	GCAATG	1 cut(s)	2213
BseNI	ACTGG	2 cut(s)	609, 1440
BseRI	GAGGAG	1 cut(s)	1420
BseSI	GKGCMC	1 cut(s)	742
BseXI	GCAGC	4 cut(s)	414, 1100, 1512, 1839
BseYI	CCCAGC	1 cut(s)	284
BsgI	GTGCAG	1 cut(s)	1718
Bsh1285I	CGRYCG	1 cut(s)	1848
BshFI	GGCC	5 cut(s)	522, 608, 833, 1113, 1642
BshTI	ACCGGT	2 cut(s)	1154, 1733
BshVI	ATCGAT	1 cut(s)	231
BsiEI	CGRYCG	1 cut(s)	1848
BsiHKAI	GWGCWC	2 cut(s)	742, 1218
BsiSI	CCGG	4 cut(s)	834, 1155, 1322, 1734
BslFI	GGGAC	3 cut(s)	680, 1823, 1953
BslI	CCNNNNNNNGG	6 cut(s)	600, 773, 998, 1478, 1638, 2002
BsmAI	GTCTC	3 cut(s)	166, 454, 1871
BsmBI	CGTCTC	1 cut(s)	166
BsmFI	GGGAC	3 cut(s)	680, 1823, 1953
BsnI	GGCC	5 cut(s)	522, 608, 833, 1113, 1642
Bsp1286I	GDGCHC	2 cut(s)	742, 1218
Bsp1720I	GCTNAGC	1 cut(s)	1593
BspACI	CCGC	5 cut(s)	354, 553, 565, 2165, 2190
BspANI	GGCC	5 cut(s)	522, 608, 833, 1113, 1642
BspDI	ATCGAT	1 cut(s)	231
BspLI	GGNNCC	6 cut(s)	26, 696, 816, 1704, 1801, 2007
BspMAI	CTGCAG	1 cut(s)	1090
BspMI	ACCTGC	2 cut(s)	860, 1036
BspPI	GGATC	8 cut(s)	115, 188, 236, 1070, 1541, 1697, 1710, 1769
BsrDI	GCAATG	1 cut(s)	2213
BsrFI	RCCGGY	2 cut(s)	1154, 1733
BsrI	ACTGG	2 cut(s)	609, 1440
BssAI	RCCGGY	2 cut(s)	1154, 1733
BssECI	CCNNGG	5 cut(s)	767, 1063, 1537, 1643, 1948
BssT1I	CCWWGG	3 cut(s)	767, 1063, 1643
Bst2UI	CCWGG	3 cut(s)	1779, 1857, 2277
Bst6I	CTCTTC	3 cut(s)	2, 1573, 2124
BstAPI	GCANNNNNTGC	2 cut(s)	386, 875
BstC8I	GCNNGC	3 cut(s)	492, 831, 1513
BstDSI	CCRYGG	1 cut(s)	1537
BstENI	CCTNNNNNAGG	1 cut(s)	771
BstH2I	RGCGCY	1 cut(s)	408
BstHHI	GCGC	1 cut(s)	407
BstMAI	GTCTC	3 cut(s)	166, 454, 1871
BstMCI	CGRYCG	1 cut(s)	1848
BstMWI	GCNNNNNNNGC	4 cut(s)	220, 386, 528, 875
BstNI	CCWGG	3 cut(s)	1779, 1857, 2277
BstNSI	RCATGY	3 cut(s)	430, 494, 1511
BstSCI	CCNGG	3 cut(s)	1777, 1855, 2275
BstSFI	CTRYAG	4 cut(s)	126, 431, 579, 1086
BstSLI	GKGCMC	1 cut(s)	742
BstV1I	GCAGC	4 cut(s)	414, 1100, 1512, 1839
BstV2I	GAAGAC	2 cut(s)	715, 2071
BstX2I	RGATCY	5 cut(s)	107, 1075, 1384, 1546, 1702
BstYI	RGATCY	5 cut(s)	107, 1075, 1384, 1546, 1702
Bsu15I	ATCGAT	1 cut(s)	231
Bsu36I	CCTNAGG	2 cut(s)	51, 1866
BsuRI	GGCC	5 cut(s)	522, 608, 833, 1113, 1642
BsuTUI	ATCGAT	1 cut(s)	231
BtgI	CCRYGG	1 cut(s)	1537
BtgZI	GCGATG	1 cut(s)	1218
BtrI	CACGTC	1 cut(s)	176
BtsIMutI	CAGTG	5 cut(s)	483, 894, 1515, 1812, 2157
BveI	ACCTGC	2 cut(s)	860, 1036
Cac8I	GCNNGC	3 cut(s)	492, 831, 1513
CaiI	CAGNNNCTG	1 cut(s)	1124
CfoI	GCGC	1 cut(s)	407
Cfr10I	RCCGGY	2 cut(s)	1154, 1733
Cfr13I	GGNCC	5 cut(s)	694, 1244, 1781, 1799, 1859
ClaI	ATCGAT	1 cut(s)	231
Csp6I	GTAC	2 cut(s)	1025, 2031
CspAI	ACCGGT	2 cut(s)	1154, 1733
CviQI	GTAC	2 cut(s)	1025, 2031
EaeI	YGGCCR	3 cut(s)	606, 831, 1640
Eam1104I	CTCTTC	3 cut(s)	2, 1573, 2124
EarI	CTCTTC	3 cut(s)	2, 1573, 2124
Ecl136II	GAGCTC	1 cut(s)	1216
Eco130I	CCWWGG	3 cut(s)	767, 1063, 1643
Eco24I	GRGCYC	1 cut(s)	1218
Eco32I	GATATC	1 cut(s)	2232
Eco47I	GGWCC	5 cut(s)	694, 1244, 1781, 1799, 1859
Eco47III	AGCGCT	1 cut(s)	406
Eco53kI	GAGCTC	1 cut(s)	1216
Eco57I	CTGAAG	1 cut(s)	709
Eco81I	CCTNAGG	2 cut(s)	51, 1866
EcoICRI	GAGCTC	1 cut(s)	1216
EcoNI	CCTNNNNNAGG	1 cut(s)	771
EcoO109I	RGGNCCY	1 cut(s)	1799
EcoRII	CCWGG	3 cut(s)	1777, 1855, 2275
EcoRV	GATATC	1 cut(s)	2232
EcoT14I	CCWWGG	3 cut(s)	767, 1063, 1643
EcoT38I	GRGCYC	1 cut(s)	1218
ErhI	CCWWGG	3 cut(s)	767, 1063, 1643
Esp3I	CGTCTC	1 cut(s)	166
FalI	AAGNNNNNCTT	2 cut(s)	391, 423
FaqI	GGGAC	3 cut(s)	680, 1823, 1953
FauI	CCCGC	2 cut(s)	2158, 2183
FblI	GTMKAC	2 cut(s)	900, 2107
Fnu4HI	GCNGC	6 cut(s)	354, 403, 553, 1089, 1501, 1853
FriOI	GRGCYC	1 cut(s)	1218
Fsp4HI	GCNGC	6 cut(s)	354, 403, 553, 1089, 1501, 1853
FspBI	CTAG	2 cut(s)	768, 1431
GlaI	GCGC	1 cut(s)	406
GluI	GCNGC	6 cut(s)	354, 403, 553, 1089, 1501, 1853
GsaI	CCCAGC	1 cut(s)	288
GsuI	CTGGAG	1 cut(s)	1423
HaeII	RGCGCY	1 cut(s)	408
HaeIII	GGCC	5 cut(s)	522, 608, 833, 1113, 1642
HapII	CCGG	4 cut(s)	834, 1155, 1322, 1734
HhaI	GCGC	1 cut(s)	407
Hin6I	GCGC	1 cut(s)	405
HinP1I	GCGC	1 cut(s)	405
HincII	GTYRAC	4 cut(s)	901, 1626, 1689, 2108
HindII	GTYRAC	4 cut(s)	901, 1626, 1689, 2108
HindIII	AAGCTT	2 cut(s)	290, 392
HinfI	GANTC	4 cut(s)	948, 1147, 1269, 1457
HpaI	GTTAAC	1 cut(s)	1689
HpaII	CCGG	4 cut(s)	834, 1155, 1322, 1734
HphI	GGTGA	5 cut(s)	169, 191, 214, 466, 2155
Hpy166II	GTNNAC	6 cut(s)	280, 740, 901, 1626, 1689, 2108
Hpy188III	TCNNGA	9 cut(s)	171, 535, 713, 774, 1572, 1662, 1742, 1865, 1932
Hpy8I	GTNNAC	6 cut(s)	280, 740, 901, 1626, 1689, 2108
HpyAV	CCTTC	7 cut(s)	39, 148, 154, 992, 1099, 1337, 1415
HpyCH4IV	ACGT	2 cut(s)	73, 175
HpyF10VI	GCNNNNNNNGC	4 cut(s)	220, 386, 528, 875
HpySE526I	ACGT	2 cut(s)	73, 175
HspAI	GCGC	1 cut(s)	405
KspAI	GTTAAC	1 cut(s)	1689
LmnI	GCTCC	4 cut(s)	92, 1213, 2011, 2278
Lsp1109I	GCAGC	4 cut(s)	414, 1100, 1512, 1839
LweI	GCATC	8 cut(s)	173, 232, 319, 376, 1327, 1783, 1908, 1962
MaeI	CTAG	2 cut(s)	768, 1431
MaeII	ACGT	2 cut(s)	73, 175
MaeIII	GTNAC	7 cut(s)	184, 454, 1253, 1585, 1964, 2073, 2267
MfeI	CAATTG	2 cut(s)	501, 1014
MflI	RGATCY	5 cut(s)	107, 1075, 1384, 1546, 1702
MhlI	GDGCHC	2 cut(s)	742, 1218
MlsI	TGGCCA	2 cut(s)	608, 1642
MluNI	TGGCCA	2 cut(s)	608, 1642
MlyI	GAGTC	1 cut(s)	1451
MmeI	TCCRAC	4 cut(s)	279, 916, 1733, 1958
Mox20I	TGGCCA	2 cut(s)	608, 1642
MroXI	GAANNNNTTC	4 cut(s)	594, 1146, 1317, 1425
MscI	TGGCCA	2 cut(s)	608, 1642
MseI	TTAA	7 cut(s)	18, 372, 438, 840, 906, 966, 1688
MslI	CAYNNNNRTG	2 cut(s)	2025, 2058
Msp20I	TGGCCA	2 cut(s)	608, 1642
MspI	CCGG	4 cut(s)	834, 1155, 1322, 1734
MspR9I	CCNGG	3 cut(s)	1779, 1857, 2277
MunI	CAATTG	2 cut(s)	501, 1014
MvaI	CCWGG	3 cut(s)	1779, 1857, 2277
MwoI	GCNNNNNNNGC	4 cut(s)	220, 386, 528, 875
NlaIV	GGNNCC	6 cut(s)	26, 696, 816, 1704, 1801, 2007
NmeAIII	GCCGAG	1 cut(s)	1929
NmuCI	GTSAC	1 cut(s)	454
NspI	RCATGY	3 cut(s)	430, 494, 1511
PaeI	GCATGC	1 cut(s)	494
PdmI	GAANNNNTTC	4 cut(s)	594, 1146, 1317, 1425
PfeI	GAWTC	3 cut(s)	948, 1147, 1269
PflMI	CCANNNNNTGG	1 cut(s)	2002
PfoI	TCCNGGA	1 cut(s)	2275
PinAI	ACCGGT	2 cut(s)	1154, 1733
PkrI	GCNGC	6 cut(s)	355, 404, 554, 1090, 1502, 1854
Ple19I	CGATCG	1 cut(s)	1848
PleI	GAGTC	1 cut(s)	1451
PpsI	GAGTC	1 cut(s)	1451
PpuMI	RGGWCCY	1 cut(s)	1799
Psp124BI	GAGCTC	1 cut(s)	1218
Psp5II	RGGWCCY	1 cut(s)	1799
Psp6I	CCWGG	3 cut(s)	1777, 1855, 2275
PspFI	CCCAGC	1 cut(s)	284
PspGI	CCWGG	3 cut(s)	1777, 1855, 2275
PspN4I	GGNNCC	6 cut(s)	26, 696, 816, 1704, 1801, 2007
PspPI	GGNCC	5 cut(s)	694, 1244, 1781, 1799, 1859
PspPPI	RGGWCCY	1 cut(s)	1799
PstI	CTGCAG	1 cut(s)	1090
PstNI	CAGNNNCTG	1 cut(s)	1124
PsuI	RGATCY	5 cut(s)	107, 1075, 1384, 1546, 1702
PvuI	CGATCG	1 cut(s)	1848
RsaI	GTAC	2 cut(s)	1026, 2032
RsaNI	GTAC	2 cut(s)	1025, 2031
RseI	CAYNNNNRTG	2 cut(s)	2025, 2058
SacI	GAGCTC	1 cut(s)	1218
SalI	GTCGAC	2 cut(s)	899, 2106
SaqAI	TTAA	7 cut(s)	18, 372, 438, 840, 906, 966, 1688
SatI	GCNGC	6 cut(s)	354, 403, 553, 1089, 1501, 1853
Sau96I	GGNCC	5 cut(s)	694, 1244, 1781, 1799, 1859
SchI	GAGTC	1 cut(s)	1451
ScrFI	CCNGG	3 cut(s)	1779, 1857, 2277
SduI	GDGCHC	2 cut(s)	742, 1218
SfaNI	GCATC	8 cut(s)	173, 232, 319, 376, 1327, 1783, 1908, 1962
SfcI	CTRYAG	4 cut(s)	126, 431, 579, 1086
SinI	GGWCC	5 cut(s)	694, 1244, 1781, 1799, 1859
SmiMI	CAYNNNNRTG	2 cut(s)	2025, 2058
SmlI	CTYRAG	2 cut(s)	1274, 1614
SmoI	CTYRAG	2 cut(s)	1274, 1614
SphI	GCATGC	1 cut(s)	494
SsiI	CCGC	5 cut(s)	354, 553, 565, 2165, 2190
SspI	AATATT	1 cut(s)	1911
SspMI	CTAG	2 cut(s)	768, 1431
SstI	GAGCTC	1 cut(s)	1218
StyD4I	CCNGG	3 cut(s)	1777, 1855, 2275
StyI	CCWWGG	3 cut(s)	767, 1063, 1643
TaiI	ACGT	2 cut(s)	76, 178
TaqII	GACCGA	1 cut(s)	682
TauI	GCSGC	2 cut(s)	356, 555
TfiI	GAWTC	3 cut(s)	948, 1147, 1269
Tru1I	TTAA	7 cut(s)	18, 372, 438, 840, 906, 966, 1688
Tru9I	TTAA	7 cut(s)	18, 372, 438, 840, 906, 966, 1688
TscAI	CASTG	5 cut(s)	490, 901, 1522, 1819, 2157
TseFI	GTSAC	1 cut(s)	454
TseI	GCWGC	4 cut(s)	402, 1088, 1500, 1852
Tsp45I	GTSAC	1 cut(s)	454
TspDTI	ATGAA	8 cut(s)	1139, 1270, 1320, 1571, 1598, 1710, 2042, 2277
TspGWI	ACGGA	6 cut(s)	374, 936, 1409, 1526, 1684, 2112
TspRI	CASTG	5 cut(s)	490, 901, 1522, 1819, 2157
Van91I	CCANNNNNTGG	1 cut(s)	2002
VneI	GTGCAC	1 cut(s)	738
VpaK11BI	GGWCC	5 cut(s)	694, 1244, 1781, 1799, 1859
XagI	CCTNNNNNAGG	1 cut(s)	771
XapI	RAATTY	2 cut(s)	1350, 1840
XceI	RCATGY	3 cut(s)	430, 494, 1511
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1238, 1785
XmaJI	CCTAGG	1 cut(s)	767
XmiI	GTMKAC	2 cut(s)	900, 2107
XmnI	GAANNNNTTC	4 cut(s)	594, 1146, 1317, 1425
XspI	CTAG	2 cut(s)	768, 1431

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.