Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005278

Physical Location & Seq

Genomic Coordinates Forward (+)

67107516 .. 67118061

Gene Structure 10546 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ41475

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGCAGAAGAGTGGGAGTGTTTCCAAGAATAGTTTGAGAGTGACGACCCCTCAGCAGTCCCTTAGGCGACTGGGCTTGTGCTCCCAAATAGCTACTGCTACTGGTGGCCAGCACTCCTCCCCAATTGTCTTCCCTGAGAAACAGAAACGACACAAGATCAAGGCTTCTAAGACTCCTACTACTCCTGCCTCTGCTGATGATCCCAATTCTCTCAAGACCTTCGAGCACAGGATTGACATTCCGGCTTCTGCTGCTGGAGATGAAAAGTCTGATTTGTTGGGTTATGCCGTCTTCTCTGGAAAGTTGGTTTTGGACAAGAGTAAAATCACTCTCGTAAACACCACTTCTACTGATCCACAACAACAAACCTCCTCCTCTACCAACCAGGAAGCTGTTGATGCTAAGCTTACAAGCAAGGCCTTAATTTGGGGTTCTCACATGCTGCATCTGGATGATGTTATCTCGGTTTCATACAATGTTGGGCTCAGACATTTTACCGTGCATTCTTATCCCTTAAAAAAGGGTTCTTTTGGCCTTTCTTGTTTCATGAAACCTCGAAGAAGTCGCAAGGACTTTCGATTCTTGGCTTCTAGCATAGAAGACGCCGTTCAATGGGTTGGTGGGTTTGCTGATCAGCAGTGTTATGTGAATTGCTTGCCTCATCCATTATTATCTTCTAAGAAGCAGGCTTCTTCAGAATTACTTCCCATTGATACTCCTCCTGAATTAATTTTCAAATGTAAGAGCCCACCAAAAATGCTTGTCATATTGAATCCACGATCGGGACGTGGCCGTTCGAGTAAGGTTTTTGACAGCATCGTTGAACCTATATTTAAGCTTGCAGGTTTCAAAGTGGAGGTAGTCAAAACAACATCTGCAGGTCATGCTAAGAAACTTGCATCTAGTGTTGACATTAGCAGCTGTCCTGATGGAATCATATGTGTTGGGGGCGATGGAATTATTAATGAGGTTCTAAATGGTTTACTTAGTAGAGACAACCAAAAAGAAGGAATTTCCATACCAATTGGAATTATACCTGCTGGATCCGATAATTCCTTAGTTTGGACGGTTCTTGGAGTTAGGGATCCAGTTTCTGCTGCAATGGCTATTGTCAAGGGTGGTCTTACTGCTACAGATGTTTTTGCTGTTGAGTGGATTCAGACGGGTGTTATCCACTTTGGGATGACAGTCTCTTATTATGGTTTTGTGAGTGATGTGTTGGAGCTGTCTGAGAAATATCAGAAGCGCTTTGGTCCTCTGCGTTATTTTGTTGCTGGAGTTCTTAAATTCTTATGCCTGCCGAAGTACAGCTATGAAGTTGAGTACCTTCCAGCATCAAAAGAGGATCTAGAAGGAAAACTCTCAGCTGAAATTGATGTGTCAGACTTGTACACAGACATTATGAGGAGATCAAATACCGATGGTATTCCAAGAGCTTCTAGTTTATCAAGTATTGACTCCATTATGACTCCTAGTCGAATGTCTGGAGGTGACTTGGATACAACCTGCAGTAGCACTCATGCTAGCATTGAACCATCTGAGTACGTGCGTGGCCTAGATCCTAAAGCAAAAAGACTGTCAATTGGAAGGACCAATATAACTGCAGAACCGGAAGTTATTCATCCTCAGCTGCCCCTGTCAACAACACCAAACTGGCCAAGGACCCGGTCAAAGTCAAGGACCGATAGAGGATGGACTGGATTGACAGCCACACATGATGCCTCTAGATCTTCTTGGGGAAACACTGGAACAAATGATAAAGAGGATATATCATCCACGCTATCCGATCCAGGCCCAATTTGGGATGCTGAGCCAAAGTGGGATACTGAACCTAATTGGGATGTGGAAAATCCTATTGAATTGCCAGGGCCGTCAGATGACGTAGAAGCAGGAACAAAGGAAGTTGTGCCCAGGTATGAAGATAAATGGGTTGTGACCAAGGGTCAGTTGCTTGGCATTCTTGTTTGCAATCATGCATGCAGAACTGTTCAGAGTTCCCAAGTGGTGGCACCAAAAGCTGAGCATGATGACAACACCTTGGATTTGCTCCTAGTTCATGGAAGTGGGCGATTAAGGCTGTTGAGGTTTTTCATGCTTCTGCAGATGGGTAGACACCTGTCACTGCCATACGTGGAAAATGTCAAGGTAAAGTCGGTGAAGATCAAGGCATCAGGAAAGCACACCCATAACGGTTGTGGCATTGATGGGGAGCTTTTTCCACTTAATGGGCAAGTCATTTCTTCGTTGCTTCCAGAACAGTGCAGACTTATTGGTCGCTCCTATAACCATCACGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006629 GO:0006643 GO:0006665 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0017050 GO:0030148 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0046467 GO:0071704 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAGK_cat

Protein Analysis

765

Amino Acids

83.57

Weight (kDa)

8.49

Isoelectric Point (pI)

36.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	252 - 382	1.7e-29	Diacylglycerol kinase catalytic domain
YegS_C	PF19279	666 - 743	1.5e-10	YegS C-terminal NAD kinase beta sandwich-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005278)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23450 AT5G23450 AT5G23450 AT5G23450 AT5G23450
fragaria_vesca	FvH4_4g37380 FvH4_4g37380 FvH4_4g37380
malus_domestica	MD13G1000100.v1.1 MD16G1005700.v1.1 MD16G1005800.v1.1
prunus_persica	Prupe.1G584800_v2.0.a1
pyrus_communis	pycom13g00010 pycom2749g00100 pycom2749g00120
rosa_chinensis	RchiOBHm_Chr4g0447291
rosa_laevigata	RLG00000005589
rosa_multiflora	Rmu_co8061930.1_g000001 Rmu_sc0017601.1_g000001 Rmu_ssc0000396.1_g000006
rosa_roxburghii	Rroxscaffold_5G00387670
rosa_rugosa	Rorug04G0379600 Rorug04G0379600 Rorug04G0379700
rosa_samantha	Rh4AG440900 Rh4BG450000 Rh4CG467100 Rh4DG448400
rosa_wichuraiana	Rw4G037730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2271
Acc36I	ACCTGC	4 cut(s)	833, 869, 1045, 1514
AccB1I	GGYRCC	1 cut(s)	2008
AccB7I	CCANNNNNTGG	2 cut(s)	2005, 2225
AccI	GTMKAC	1 cut(s)	2110
AclWI	GGATC	9 cut(s)	194, 347, 1038, 1051, 1079, 1092, 1353, 1553, 1781
AcoI	YGGCCR	3 cut(s)	106, 790, 1655
AcsI	RAATTY	2 cut(s)	1011, 1286
AcuI	CTGAAG	1 cut(s)	678
AcyI	GRCGYC	1 cut(s)	603
AfaI	GTAC	4 cut(s)	1307, 1325, 1391, 1544
AfeI	AGCGCT	1 cut(s)	1247
AfiI	CCNNNNNNNGG	7 cut(s)	426, 612, 782, 1062, 1801, 2005, 2225
AgsI	TTSAA	7 cut(s)	611, 736, 772, 824, 850, 1532, 1859
AhdI	GACNNNNNGTC	2 cut(s)	1473, 1941
AjiI	CACGTC	2 cut(s)	788, 2293
AjnI	CCWGG	4 cut(s)	384, 1789, 1864, 1910
Alw21I	GWGCWC	2 cut(s)	83, 228
Alw26I	GTCTC	2 cut(s)	987, 1195
AlwI	GGATC	9 cut(s)	194, 347, 1038, 1051, 1079, 1092, 1353, 1553, 1781
AlwNI	CAGNNNCTG	1 cut(s)	1094
Aor51HI	AGCGCT	1 cut(s)	1247
AoxI	GGCC	8 cut(s)	106, 417, 532, 790, 1552, 1655, 1792, 1868
ApeKI	GCWGC	5 cut(s)	251, 442, 918, 1097, 1630
ApoI	RAATTY	2 cut(s)	1011, 1286
ArsI	GACNNNNNNTTYG	2 cut(s)	1562, 1594
AseI	ATTAAT	2 cut(s)	728, 963
Asp700I	GAANNNNTTC	2 cut(s)	702, 1617
AspLEI	GCGC	1 cut(s)	1248
AspS9I	GGNCC	6 cut(s)	1253, 1590, 1662, 1680, 1793, 1868
AsuC2I	CCSGG	1 cut(s)	1666
AsuHPI	GGTGA	2 cut(s)	1502, 2167
AsuNHI	GCTAGC	1 cut(s)	1523
AvaII	GGWCC	4 cut(s)	1253, 1590, 1662, 1680
AxyI	CCTNAGG	1 cut(s)	62
BaeGI	GKGCMC	1 cut(s)	1911
BalI	TGGCCA	2 cut(s)	108, 1657
BamHI	GGATCC	2 cut(s)	1043, 1084
BanI	GGYRCC	1 cut(s)	2008
BanII	GRGCYC	2 cut(s)	486, 749
BbsI	GAAGAC	3 cut(s)	121, 283, 606
Bbv12I	GWGCWC	2 cut(s)	83, 228
BbvCI	CCTCAGC	2 cut(s)	51, 1626
BbvI	GCAGC	5 cut(s)	238, 429, 930, 1084, 1617
BccI	CCATC	8 cut(s)	923, 947, 1415, 1543, 1686, 2098, 2198, 2295
BceAI	ACGGC	4 cut(s)	272, 590, 777, 1855
BciT130I	CCWGG	4 cut(s)	386, 1791, 1866, 1912
BciVI	GTATCC	2 cut(s)	1492, 1816
BclI	TGATCA	1 cut(s)	631
BcnI	CCSGG	1 cut(s)	1666
BcoDI	GTCTC	2 cut(s)	987, 1195
BfaI	CTAG	9 cut(s)	591, 903, 1349, 1440, 1473, 1524, 1556, 1725, 2051
BfmI	CTRYAG	5 cut(s)	876, 1131, 1507, 1602, 2099
BfoI	RGCGCY	1 cut(s)	1249
BfuAI	ACCTGC	4 cut(s)	833, 869, 1045, 1514
BfuI	GTATCC	2 cut(s)	1492, 1816
BglII	AGATCT	1 cut(s)	1727
BisI	GCNGC	5 cut(s)	252, 443, 919, 1098, 1631
BlpI	GCTNAGC	3 cut(s)	402, 1809, 2019
BlsI	GCNGC	5 cut(s)	253, 444, 920, 1099, 1632
Bme1390I	CCNGG	5 cut(s)	386, 1666, 1791, 1866, 1912
Bme18I	GGWCC	4 cut(s)	1253, 1590, 1662, 1680
BmeRI	GACNNNNNGTC	2 cut(s)	1473, 1941
BmgBI	CACGTC	2 cut(s)	788, 2293
BmgT120I	GGNCC	6 cut(s)	1253, 1590, 1662, 1680, 1793, 1868
BmiI	GGNNCC	4 cut(s)	1045, 1086, 1664, 2010
BmrFI	CCNGG	5 cut(s)	386, 1666, 1791, 1866, 1912
BmrI	ACTGGG	1 cut(s)	80
BmsI	GCATC	8 cut(s)	388, 454, 825, 908, 1343, 1708, 1795, 2177
BmtI	GCTAGC	1 cut(s)	1527
BmuI	ACTGGG	1 cut(s)	80
BoxI	GACNNNNGTC	1 cut(s)	2116
BpiI	GAAGAC	3 cut(s)	121, 283, 606
BpmI	CTGGAG	3 cut(s)	276, 1296, 1506
Bpu10I	CCTNAGC	2 cut(s)	51, 1626
Bpu1102I	GCTNAGC	3 cut(s)	402, 1809, 2019
BpuEI	CTTGAG	1 cut(s)	197
BpuMI	CCSGG	1 cut(s)	1666
BsaAI	YACGTR	2 cut(s)	1546, 2131
BsaHI	GRCGYC	1 cut(s)	603
BsaJI	CCNNGG	5 cut(s)	1658, 1865, 1910, 1938, 2037
BsaWI	WCCGGW	1 cut(s)	1609
Bsc4I	CCNNNNNNNGG	7 cut(s)	426, 612, 782, 1062, 1801, 2005, 2225
Bse1I	ACTGG	6 cut(s)	75, 106, 1088, 1658, 1702, 1750
Bse21I	CCTNAGG	1 cut(s)	62
Bse3DI	GCAATG	1 cut(s)	1107
BseBI	CCWGG	4 cut(s)	386, 1791, 1866, 1912
BseDI	CCNNGG	5 cut(s)	1658, 1865, 1910, 1938, 2037
BseGI	GGATG	8 cut(s)	457, 661, 1188, 1621, 1697, 1772, 1810, 1846
BseLI	CCNNNNNNNGG	7 cut(s)	426, 612, 782, 1062, 1801, 2005, 2225
BseMI	GCAATG	1 cut(s)	1107
BseMII	CTCAG	9 cut(s)	65, 126, 499, 1221, 1377, 1530, 1640, 1800, 2010
BseNI	ACTGG	6 cut(s)	75, 106, 1088, 1658, 1702, 1750
BseRI	GAGGAG	5 cut(s)	106, 361, 364, 708, 1420
BseSI	GKGCMC	1 cut(s)	1911
BseXI	GCAGC	5 cut(s)	238, 429, 930, 1084, 1617
BsgI	GTGCAG	1 cut(s)	2281
Bsh1285I	CGRYCG	1 cut(s)	782
BshFI	GGCC	8 cut(s)	108, 419, 534, 792, 1554, 1657, 1794, 1870
BshNI	GGYRCC	1 cut(s)	2008
BsiEI	CGRYCG	1 cut(s)	782
BsiHKAI	GWGCWC	2 cut(s)	83, 228
BsiSI	CCGG	3 cut(s)	242, 1610, 1666
BslFI	GGGAC	2 cut(s)	43, 798
BslI	CCNNNNNNNGG	7 cut(s)	426, 612, 782, 1062, 1801, 2005, 2225
BsmAI	GTCTC	2 cut(s)	987, 1195
BsmFI	GGGAC	2 cut(s)	43, 798
BsmI	GAATGC	2 cut(s)	502, 1956
BsnI	GGCC	8 cut(s)	108, 419, 534, 792, 1554, 1657, 1794, 1870
Bsp1286I	GDGCHC	5 cut(s)	83, 228, 486, 749, 1911
Bsp1407I	TGTACA	1 cut(s)	1389
Bsp1720I	GCTNAGC	3 cut(s)	402, 1809, 2019
BspANI	GGCC	8 cut(s)	108, 419, 534, 792, 1554, 1657, 1794, 1870
BspCNI	CTCAG	9 cut(s)	64, 127, 498, 1222, 1376, 1531, 1639, 1801, 2011
BspHI	TCATGA	1 cut(s)	546
BspLI	GGNNCC	4 cut(s)	1045, 1086, 1664, 2010
BspMAI	CTGCAG	4 cut(s)	880, 1511, 1606, 2103
BspMI	ACCTGC	4 cut(s)	833, 869, 1045, 1514
BspOI	GCTAGC	1 cut(s)	1527
BspPI	GGATC	9 cut(s)	194, 347, 1038, 1051, 1079, 1092, 1353, 1553, 1781
BspT107I	GGYRCC	1 cut(s)	2008
BsrDI	GCAATG	1 cut(s)	1107
BsrGI	TGTACA	1 cut(s)	1389
BsrI	ACTGG	6 cut(s)	75, 106, 1088, 1658, 1702, 1750
BssECI	CCNNGG	5 cut(s)	1658, 1865, 1910, 1938, 2037
BssNI	GRCGYC	1 cut(s)	603
BssT1I	CCWWGG	3 cut(s)	1658, 1938, 2037
Bst2UI	CCWGG	4 cut(s)	386, 1791, 1866, 1912
Bst4CI	ACNGT	7 cut(s)	499, 1069, 1189, 1578, 1987, 2192, 2259
Bst6I	CTCTTC	1 cut(s)	2
BstACI	GRCGYC	1 cut(s)	603
BstAPI	GCANNNNNTGC	1 cut(s)	884
BstAUI	TGTACA	1 cut(s)	1389
BstBAI	YACGTR	2 cut(s)	1546, 2131
BstC8I	GCNNGC	7 cut(s)	110, 656, 687, 840, 1298, 1525, 1978
BstF5I	GGATG	8 cut(s)	457, 661, 1188, 1621, 1697, 1772, 1810, 1846
BstH2I	RGCGCY	1 cut(s)	1249
BstHHI	GCGC	1 cut(s)	1248
BstMAI	GTCTC	2 cut(s)	987, 1195
BstMCI	CGRYCG	1 cut(s)	782
BstMWI	GCNNNNNNNGC	6 cut(s)	251, 398, 884, 1103, 2015, 2074
BstNI	CCWGG	4 cut(s)	386, 1791, 1866, 1912
BstNSI	RCATGY	2 cut(s)	442, 1980
BstPAI	GACNNNNGTC	1 cut(s)	2116
BstSCI	CCNGG	5 cut(s)	384, 1664, 1789, 1864, 1910
BstSFI	CTRYAG	5 cut(s)	876, 1131, 1507, 1602, 2099
BstSLI	GKGCMC	1 cut(s)	1911
BstV1I	GCAGC	5 cut(s)	238, 429, 930, 1084, 1617
BstV2I	GAAGAC	3 cut(s)	121, 283, 606
BstX2I	RGATCY	5 cut(s)	1043, 1084, 1345, 1558, 1727
BstYI	RGATCY	5 cut(s)	1043, 1084, 1345, 1558, 1727
Bsu36I	CCTNAGG	1 cut(s)	62
BsuI	GTATCC	2 cut(s)	1492, 1816
BsuRI	GGCC	8 cut(s)	108, 419, 534, 792, 1554, 1657, 1794, 1870
BtgZI	GCGATG	1 cut(s)	966
BtrI	CACGTC	2 cut(s)	788, 2293
BtsCI	GGATG	8 cut(s)	457, 661, 1188, 1621, 1697, 1772, 1810, 1846
BtsI	GCAGTG	2 cut(s)	644, 2120
BtsIMutI	CAGTG	4 cut(s)	644, 1743, 2120, 2264
BveI	ACCTGC	4 cut(s)	833, 869, 1045, 1514
Cac8I	GCNNGC	7 cut(s)	110, 656, 687, 840, 1298, 1525, 1978
CaiI	CAGNNNCTG	1 cut(s)	1094
CciI	TCATGA	1 cut(s)	546
CfoI	GCGC	1 cut(s)	1248
Cfr13I	GGNCC	6 cut(s)	1253, 1590, 1662, 1680, 1793, 1868
CseI	GACGC	1 cut(s)	611
Csp6I	GTAC	4 cut(s)	1306, 1324, 1390, 1543
CviQI	GTAC	4 cut(s)	1306, 1324, 1390, 1543
DrdI	GACNNNNNNGTC	1 cut(s)	2271
DriI	GACNNNNNGTC	2 cut(s)	1473, 1941
DseDI	GACNNNNNNGTC	1 cut(s)	2271
EaeI	YGGCCR	3 cut(s)	106, 790, 1655
Eam1104I	CTCTTC	1 cut(s)	2
Eam1105I	GACNNNNNGTC	2 cut(s)	1473, 1941
EarI	CTCTTC	1 cut(s)	2
Eco130I	CCWWGG	3 cut(s)	1658, 1938, 2037
Eco147I	AGGCCT	1 cut(s)	419
Eco24I	GRGCYC	2 cut(s)	486, 749
Eco47I	GGWCC	4 cut(s)	1253, 1590, 1662, 1680
Eco47III	AGCGCT	1 cut(s)	1247
Eco57I	CTGAAG	1 cut(s)	678
Eco81I	CCTNAGG	1 cut(s)	62
EcoO109I	RGGNCCY	1 cut(s)	1662
EcoRII	CCWGG	4 cut(s)	384, 1789, 1864, 1910
EcoT14I	CCWWGG	3 cut(s)	1658, 1938, 2037
EcoT22I	ATGCAT	1 cut(s)	1978
EcoT38I	GRGCYC	2 cut(s)	486, 749
ErhI	CCWWGG	3 cut(s)	1658, 1938, 2037
FaqI	GGGAC	2 cut(s)	43, 798
FauNDI	CATATG	1 cut(s)	938
FbaI	TGATCA	1 cut(s)	631
FblI	GTMKAC	1 cut(s)	2110
Fnu4HI	GCNGC	5 cut(s)	252, 443, 919, 1098, 1631
FokI	GGATG	8 cut(s)	464, 648, 1195, 1608, 1704, 1759, 1817, 1853
FriOI	GRGCYC	2 cut(s)	486, 749
Fsp4HI	GCNGC	5 cut(s)	252, 443, 919, 1098, 1631
FspBI	CTAG	9 cut(s)	591, 903, 1349, 1440, 1473, 1524, 1556, 1725, 2051
GlaI	GCGC	1 cut(s)	1247
GluI	GCNGC	5 cut(s)	252, 443, 919, 1098, 1631
GsuI	CTGGAG	3 cut(s)	276, 1296, 1506
HaeII	RGCGCY	1 cut(s)	1249
HaeIII	GGCC	8 cut(s)	108, 419, 534, 792, 1554, 1657, 1794, 1870
HapII	CCGG	3 cut(s)	242, 1610, 1666
HgaI	GACGC	1 cut(s)	611
HhaI	GCGC	1 cut(s)	1248
Hin1I	GRCGYC	1 cut(s)	603
Hin6I	GCGC	1 cut(s)	1246
HinP1I	GCGC	1 cut(s)	1246
HincII	GTYRAC	2 cut(s)	910, 1641
HindII	GTYRAC	2 cut(s)	910, 1641
HindIII	AAGCTT	2 cut(s)	404, 836
HinfI	GANTC	7 cut(s)	172, 579, 772, 933, 1156, 1457, 1468
HpaII	CCGG	3 cut(s)	242, 1610, 1666
HphI	GGTGA	2 cut(s)	1502, 2167
Hpy166II	GTNNAC	6 cut(s)	337, 910, 983, 1392, 1641, 2111
Hpy8I	GTNNAC	6 cut(s)	337, 910, 983, 1392, 1641, 2111
HpyAV	CCTTC	5 cut(s)	229, 1001, 1337, 1346, 1581
HpyCH4III	ACNGT	7 cut(s)	499, 1069, 1189, 1578, 1987, 2192, 2259
HpyCH4IV	ACGT	5 cut(s)	787, 1545, 1881, 2130, 2292
HpyF10VI	GCNNNNNNNGC	6 cut(s)	251, 398, 884, 1103, 2015, 2074
HpySE526I	ACGT	5 cut(s)	787, 1545, 1881, 2130, 2292
Hsp92I	GRCGYC	1 cut(s)	603
HspAI	GCGC	1 cut(s)	1246
Ksp22I	TGATCA	1 cut(s)	631
LmnI	GCTCC	5 cut(s)	86, 1222, 2052, 2209, 2282
Lsp1109I	GCAGC	5 cut(s)	238, 429, 930, 1084, 1617
LweI	GCATC	8 cut(s)	388, 454, 825, 908, 1343, 1708, 1795, 2177
MaeI	CTAG	9 cut(s)	591, 903, 1349, 1440, 1473, 1524, 1556, 1725, 2051
MaeII	ACGT	5 cut(s)	787, 1545, 1881, 2130, 2292
MaeIII	GTNAC	4 cut(s)	40, 1490, 1933, 2118
MfeI	CAATTG	3 cut(s)	123, 1023, 1581
MflI	RGATCY	5 cut(s)	1043, 1084, 1345, 1558, 1727
MhlI	GDGCHC	5 cut(s)	83, 228, 486, 749, 1911
MlsI	TGGCCA	2 cut(s)	108, 1657
MluNI	TGGCCA	2 cut(s)	108, 1657
MlyI	GAGTC	3 cut(s)	166, 1451, 1462
MmeI	TCCRAC	1 cut(s)	1200
Mox20I	TGGCCA	2 cut(s)	108, 1657
Mph1103I	ATGCAT	1 cut(s)	1978
MroXI	GAANNNNTTC	2 cut(s)	702, 1617
MscI	TGGCCA	2 cut(s)	108, 1657
MseI	TTAA	8 cut(s)	422, 515, 728, 834, 963, 1284, 2072, 2223
MslI	CAYNNNNRTG	2 cut(s)	450, 2061
Msp20I	TGGCCA	2 cut(s)	108, 1657
MspA1I	CMGCKG	3 cut(s)	921, 1367, 1630
MspI	CCGG	3 cut(s)	242, 1610, 1666
MspR9I	CCNGG	5 cut(s)	386, 1666, 1791, 1866, 1912
MunI	CAATTG	3 cut(s)	123, 1023, 1581
Mva1269I	GAATGC	2 cut(s)	502, 1956
MvaI	CCWGG	4 cut(s)	386, 1791, 1866, 1912
MwoI	GCNNNNNNNGC	6 cut(s)	251, 398, 884, 1103, 2015, 2074
NciI	CCSGG	1 cut(s)	1666
NdeI	CATATG	1 cut(s)	938
NheI	GCTAGC	1 cut(s)	1523
NlaIV	GGNNCC	4 cut(s)	1045, 1086, 1664, 2010
NmuCI	GTSAC	4 cut(s)	40, 1490, 1933, 2118
NsiI	ATGCAT	1 cut(s)	1978
NspI	RCATGY	2 cut(s)	442, 1980
PaeI	GCATGC	1 cut(s)	1980
PagI	TCATGA	1 cut(s)	546
PceI	AGGCCT	1 cut(s)	419
PcsI	WCGNNNNNNNCGW	1 cut(s)	784
PctI	GAATGC	2 cut(s)	502, 1956
PdmI	GAANNNNTTC	2 cut(s)	702, 1617
PfeI	GAWTC	4 cut(s)	579, 772, 933, 1156
PflFI	GACNNNGTC	1 cut(s)	1666
PflMI	CCANNNNNTGG	2 cut(s)	2005, 2225
PkrI	GCNGC	5 cut(s)	253, 444, 920, 1099, 1632
Ple19I	CGATCG	1 cut(s)	782
PleI	GAGTC	3 cut(s)	166, 1451, 1462
PpsI	GAGTC	3 cut(s)	166, 1451, 1462
Ppu21I	YACGTR	2 cut(s)	1546, 2131
PpuMI	RGGWCCY	1 cut(s)	1662
PshAI	GACNNNNGTC	1 cut(s)	2116
PshBI	ATTAAT	2 cut(s)	728, 963
Psp5II	RGGWCCY	1 cut(s)	1662
Psp6I	CCWGG	4 cut(s)	384, 1789, 1864, 1910
PspGI	CCWGG	4 cut(s)	384, 1789, 1864, 1910
PspN4I	GGNNCC	4 cut(s)	1045, 1086, 1664, 2010
PspPI	GGNCC	6 cut(s)	1253, 1590, 1662, 1680, 1793, 1868
PspPPI	RGGWCCY	1 cut(s)	1662
PstI	CTGCAG	4 cut(s)	880, 1511, 1606, 2103
PstNI	CAGNNNCTG	1 cut(s)	1094
PsuI	RGATCY	5 cut(s)	1043, 1084, 1345, 1558, 1727
PsyI	GACNNNGTC	1 cut(s)	1666
PvuI	CGATCG	1 cut(s)	782
PvuII	CAGCTG	3 cut(s)	921, 1367, 1630
RsaI	GTAC	4 cut(s)	1307, 1325, 1391, 1544
RsaNI	GTAC	4 cut(s)	1306, 1324, 1390, 1543
RseI	CAYNNNNRTG	2 cut(s)	450, 2061
SaqAI	TTAA	8 cut(s)	422, 515, 728, 834, 963, 1284, 2072, 2223
SatI	GCNGC	5 cut(s)	252, 443, 919, 1098, 1631
Sau96I	GGNCC	6 cut(s)	1253, 1590, 1662, 1680, 1793, 1868
SchI	GAGTC	3 cut(s)	166, 1451, 1462
ScrFI	CCNGG	5 cut(s)	386, 1666, 1791, 1866, 1912
SduI	GDGCHC	5 cut(s)	83, 228, 486, 749, 1911
SfaNI	GCATC	8 cut(s)	388, 454, 825, 908, 1343, 1708, 1795, 2177
SfcI	CTRYAG	5 cut(s)	876, 1131, 1507, 1602, 2099
SinI	GGWCC	4 cut(s)	1253, 1590, 1662, 1680
SmiMI	CAYNNNNRTG	2 cut(s)	450, 2061
SmlI	CTYRAG	1 cut(s)	212
SmoI	CTYRAG	1 cut(s)	212
SphI	GCATGC	1 cut(s)	1980
SseBI	AGGCCT	1 cut(s)	419
SspMI	CTAG	9 cut(s)	591, 903, 1349, 1440, 1473, 1524, 1556, 1725, 2051
StuI	AGGCCT	1 cut(s)	419
StyD4I	CCNGG	5 cut(s)	384, 1664, 1789, 1864, 1910
StyI	CCWWGG	3 cut(s)	1658, 1938, 2037
TaaI	ACNGT	7 cut(s)	499, 1069, 1189, 1578, 1987, 2192, 2259
TaiI	ACGT	5 cut(s)	790, 1548, 1884, 2133, 2295
TaqI	TCGA	5 cut(s)	222, 556, 577, 797, 1477
TaqII	GACCGA	1 cut(s)	1697
TatI	WGTACW	2 cut(s)	1305, 1389
TfiI	GAWTC	4 cut(s)	579, 772, 933, 1156
Tru1I	TTAA	8 cut(s)	422, 515, 728, 834, 963, 1284, 2072, 2223
Tru9I	TTAA	8 cut(s)	422, 515, 728, 834, 963, 1284, 2072, 2223
TscAI	CASTG	4 cut(s)	644, 1750, 2127, 2264
TseFI	GTSAC	4 cut(s)	40, 1490, 1933, 2118
TseI	GCWGC	5 cut(s)	251, 442, 918, 1097, 1630
Tsp45I	GTSAC	4 cut(s)	40, 1490, 1933, 2118
TspDTI	ATGAA	9 cut(s)	276, 459, 535, 563, 1329, 1610, 1932, 2045, 2080
TspRI	CASTG	4 cut(s)	644, 1750, 2127, 2264
Tth111I	GACNNNGTC	1 cut(s)	1666
Van91I	CCANNNNNTGG	2 cut(s)	2005, 2225
VpaK11BI	GGWCC	4 cut(s)	1253, 1590, 1662, 1680
VspI	ATTAAT	2 cut(s)	728, 963
XapI	RAATTY	2 cut(s)	1011, 1286
XbaI	TCTAGA	2 cut(s)	1348, 1724
XceI	RCATGY	2 cut(s)	442, 1980
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1797, 2192
XmiI	GTMKAC	1 cut(s)	2110
XmnI	GAANNNNTTC	2 cut(s)	702, 1617
XspI	CTAG	9 cut(s)	591, 903, 1349, 1440, 1473, 1524, 1556, 1725, 2051
Zsp2I	ATGCAT	1 cut(s)	1978

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.