Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0005278

Physical Location & Seq

Genomic Coordinates Reverse (-)

10113 .. 17802

Gene Structure 7690 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1000100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGCAGAAGAGTGGGAGTGTTTCCAAGAACAGTTTGAGAGTAACGACGCCCCAGCAGTCTCTCAGGCGACTGGGATTGTGCTCCCAGATAGCTACTGCAACTGGGGGCCAACACTCCTCCCCAATTGTCTTCCCTGAGAAACAGAAGCGACAGAAGATCAAGGCTTCCAAGACTCCAACCCCTGCCTCCACTGAGGATCCCAACACACTAAAGACCTTCGAGCACAGGATTGACATTCCGGCTTCTGCTGCTGGAGATGAAAAGTCTGATCTGTTGGGTTATGTCGTCTTCACCGGAAAATTAGTGTTGGACAAAAGAAAAACCAGCAGTATAAACACTTCTTCTTCTACTGATGCACAACAAACCACCTCCTCCTCTGCTGATATTCCCAACCAGGAAGCTGTTGCTGGCAAGCTTACCAGTAAGGCTTTAATTTGGGGTTCTCACATGCTGCATCTTGATGATGTCATCTCAGTTTCGTACCATGTTGGTCTCAGACATTTCACCGTGCATTCTTATCCCTTGAAAAAGGGTTCTTGTGGCCTTCCATGTTTTATAAAGCCTCGCAGAAGACGTAAGGACTTTCGATTCTCGGCTTCTAGCATAGAAGAGGCAGTTCAGTGGGTTGGGGGGTTTGCTGATCAGCAGTGTTATGTGAATTGCTTGCCTCATCCATTGCTATCTTCTAAAAAGCAGGCTTCTTCAGAATTAATCCCAATTGATACTCCTCCTGAATTAATTTTCAAATGTAAGAGTCCGCCAAAAATGCTTGTTATATTAAATCCACGATCCGGACGTGGCCGTTCAAGTAAGGTTTTTCACAACGTCGTTGAACCTATATTTAAGCTTGCAGGTTTCAAAGTGGAGGTAGTTAAAACAACGTCTGCAGGTCATGCTAGGAAACTTGCCTCTACTGTTGACATTAGCACTTGTCCTGATGGAATCATTTGTGTTGGGGGTGATGGAATTATTAATGAGGTTCTAAATGGTTTACTAAGTAGAGACAACCAACAAGAAGGAATTTCTATACCAATTGGGATTATACCTGCTGGTTCTGATAATTCATTAGTTTGGACAGTCCTTGGAGTTAGAGATCCAGTTTCTGCTGCAATGGCCATTGTCAAGGGGGGTCTTACAGCTACAGATGTTTTTGCTGTTGAGTGGATTCAGACTGGCGTTATTCACTTTGGGATGACAGTCTCATATTATGGTTTTGTGAGTGATGTATTGGAGCTCTCTGAGAAATACCAAAAGCGCTTTGGTCCTCTTCGTTATTTTGTTGCTGGATTTCTCAAATTCTTGTGCCTACCAAAGTACAGCTATGAAGTTGAGTATCTTCCAGCTTTAAAAGAGGACGTAGAAGGAAAACTCTCAGAAAGGGAAGTAGTTGACATGTCAGAACTATACAGAGACATCATGAGGAGATCAAACACGGATGGGATTCCAAGAGCCTCTAGTTTGTCAAGTATCGACTCCATCATGACTCCTACTCGAATGTCTGGTGGAGACTTGGATACAAACTGCAGTAGCAGTCATGCTAGCATTGAACCATCTGAGTACGTACGTGGCCTAGATCCGAAATCAAAACGACTTTCAACGGGAAGGAGCAATGTAACTGCAGAGCCAGAAGTTATTCATCCTCAGCTTCCTCTGTCAACAACTCCAAATTGGCCAAGGACCCGCTCTAAGTCGAGAGCAGATAAAGGATGGTCTGGATTGACAGCCACACATGATGCCTCCAGATCTTCTTGGTGCAATGCTGGAACAAATGATAAAGAGGATATATCATCAACGCTGTCTGATCCAGGTCCAATTTGGGATGCGGAACCCAAGTGGGACAGTGAACCTAATTGGGATGTGGAAAATCCTATTGAATTACCAGGGCCATCAGATGATGTGGAAGCAGGAAAAAAGGAAGTTGTTCCTAGGTATGAAGATAAATGGGTTGTTACGAAGGGTCAATTCCTCGGTATTCTGGTTTGCAATCATGCTTGCAGAACTGTTCAGAGTTCTCAAGTGGTGGCGCCAAAAGCTGAGCATGATGACAACACCTTGGATTTGCTCCTAGTTCATGGAAGTGGGCGGTTAAGGCTTTTGAGATTTTTCATGCTACTGCAGATGGGTAAACACCTTTCACTGCCATACGTGGAAAATGTCAAGGCAAAGTCAGTGAAGATTAAGGTATCAGGAAAGCACACCCAAAATGGTTGTGGTATTGATGGAGAGCTTTTTCCACTCAACGGGCAAGTCATTTCTTCTTTGCTTCCAGAACAGTGCAGACTTATTGGTCGCTCCCTTCCCGCTAACCCTCACGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006629 GO:0006643 GO:0006665 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0017050 GO:0030148 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0046467 GO:0071704 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAGK_cat

Protein Analysis

773

Amino Acids

84.45

Weight (kDa)

8.75

Isoelectric Point (pI)

37.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	255 - 385	7.5e-30	Diacylglycerol kinase catalytic domain
YegS_C	PF19279	670 - 748	8.8e-11	YegS C-terminal NAD kinase beta sandwich-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005278)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23450 AT5G23450 AT5G23450 AT5G23450 AT5G23450
fragaria_vesca	FvH4_4g37380 FvH4_4g37380 FvH4_4g37380
malus_domestica	MD13G1000100.v1.1 MD16G1005700.v1.1 MD16G1005800.v1.1
prunus_persica	Prupe.1G584800_v2.0.a1
pyrus_communis	pycom13g00010 pycom2749g00100 pycom2749g00120
rosa_chinensis	RchiOBHm_Chr4g0447291
rosa_laevigata	RLG00000005589
rosa_multiflora	Rmu_co8061930.1_g000001 Rmu_sc0017601.1_g000001 Rmu_ssc0000396.1_g000006
rosa_roxburghii	Rroxscaffold_5G00387670
rosa_rugosa	Rorug04G0379600 Rorug04G0379600 Rorug04G0379700
rosa_samantha	Rh4AG440900 Rh4BG450000 Rh4CG467100 Rh4DG448400
rosa_wichuraiana	Rw4G037730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	557
AasI	GACNNNNNNGTC	1 cut(s)	2286
Acc36I	ACCTGC	3 cut(s)	842, 878, 1054
AccB1I	GGYRCC	1 cut(s)	2023
AccBSI	CCGCTC	1 cut(s)	1683
AccIII	TCCGGA	1 cut(s)	791
AciI	CCGC	5 cut(s)	758, 1681, 1823, 2083, 2301
AclWI	GGATC	6 cut(s)	191, 204, 783, 1088, 1568, 1796
AcoI	YGGCCR	3 cut(s)	799, 1113, 1670
AcsI	RAATTY	2 cut(s)	1020, 1295
AcuI	CTGAAG	1 cut(s)	687
AcyI	GRCGYC	2 cut(s)	47, 2024
AfaI	GTAC	4 cut(s)	482, 1316, 1559, 1563
AfeI	AGCGCT	1 cut(s)	1256
AfiI	CCNNNNNNNGG	2 cut(s)	791, 2240
AflIII	ACRYGT	1 cut(s)	1392
AgsI	TTSAA	8 cut(s)	526, 745, 807, 833, 859, 1547, 1596, 1874
AjiI	CACGTC	2 cut(s)	797, 2314
AjnI	CCWGG	3 cut(s)	393, 1804, 1879
Alw21I	GWGCWC	3 cut(s)	83, 225, 1236
Alw26I	GTCTC	6 cut(s)	63, 497, 996, 1204, 1404, 1500
AlwI	GGATC	6 cut(s)	191, 204, 783, 1088, 1568, 1796
AlwNI	CAGNNNCTG	1 cut(s)	1103
Aor13HI	TCCGGA	1 cut(s)	791
Aor51HI	AGCGCT	1 cut(s)	1256
AoxI	GGCC	7 cut(s)	106, 541, 799, 1113, 1567, 1670, 1883
ApeKI	GCWGC	3 cut(s)	248, 451, 1106
ApoI	RAATTY	2 cut(s)	1020, 1295
AseI	ATTAAT	3 cut(s)	710, 737, 972
Asp700I	GAANNNNTTC	2 cut(s)	1632, 1920
AspA2I	CCTAGG	1 cut(s)	1925
AspLEI	GCGC	2 cut(s)	1257, 2026
AspS9I	GGNCC	5 cut(s)	106, 1262, 1677, 1808, 1883
AsuHPI	GGTGA	3 cut(s)	283, 496, 971
AsuNHI	GCTAGC	1 cut(s)	1538
AvaII	GGWCC	3 cut(s)	1262, 1677, 1808
AvrII	CCTAGG	1 cut(s)	1925
BalI	TGGCCA	2 cut(s)	1115, 1672
BamHI	GGATCC	1 cut(s)	196
BanI	GGYRCC	1 cut(s)	2023
BanII	GRGCYC	1 cut(s)	1236
BarI	GAAGNNNNNNTAC	2 cut(s)	322, 354
BbsI	GAAGAC	3 cut(s)	121, 280, 577
Bbv12I	GWGCWC	3 cut(s)	83, 225, 1236
BbvCI	CCTCAGC	1 cut(s)	1641
BbvI	GCAGC	3 cut(s)	235, 438, 1093
BccI	CCATC	9 cut(s)	932, 956, 1430, 1484, 1558, 1701, 1894, 2113, 2213
BceAI	ACGGC	1 cut(s)	786
BciT130I	CCWGG	3 cut(s)	395, 1806, 1881
BciVI	GTATCC	1 cut(s)	1507
BclI	TGATCA	1 cut(s)	640
BcoDI	GTCTC	6 cut(s)	63, 497, 996, 1204, 1404, 1500
BfaI	CTAG	7 cut(s)	600, 897, 1455, 1539, 1571, 1926, 2066
BfmI	CTRYAG	5 cut(s)	885, 1140, 1522, 1617, 2114
BfoI	RGCGCY	2 cut(s)	1258, 2027
BfuAI	ACCTGC	3 cut(s)	842, 878, 1054
BfuI	GTATCC	1 cut(s)	1507
BglII	AGATCT	1 cut(s)	1742
BisI	GCNGC	3 cut(s)	249, 452, 1107
BlnI	CCTAGG	1 cut(s)	1925
BlpI	GCTNAGC	1 cut(s)	2034
BlsI	GCNGC	3 cut(s)	250, 453, 1108
Bme1390I	CCNGG	3 cut(s)	395, 1806, 1881
Bme18I	GGWCC	3 cut(s)	1262, 1677, 1808
BmgBI	CACGTC	2 cut(s)	797, 2314
BmgT120I	GGNCC	5 cut(s)	106, 1262, 1677, 1808, 1883
BmiI	GGNNCC	5 cut(s)	107, 198, 1679, 1827, 2025
BmrFI	CCNGG	3 cut(s)	395, 1806, 1881
BmrI	ACTGGG	2 cut(s)	80, 111
BmsI	GCATC	4 cut(s)	343, 463, 1723, 1810
BmtI	GCTAGC	1 cut(s)	1542
BmuI	ACTGGG	2 cut(s)	80, 111
BpiI	GAAGAC	3 cut(s)	121, 280, 577
BpmI	CTGGAG	2 cut(s)	273, 1723
Bpu10I	CCTNAGC	1 cut(s)	1641
Bpu1102I	GCTNAGC	1 cut(s)	2034
BpuEI	CTTGAG	1 cut(s)	1998
BsaAI	YACGTR	3 cut(s)	1561, 1565, 2146
BsaHI	GRCGYC	2 cut(s)	47, 2024
BsaI	GGTCTC	1 cut(s)	497
BsaJI	CCNNGG	6 cut(s)	1081, 1673, 1880, 1925, 1966, 2052
BsaWI	WCCGGW	2 cut(s)	293, 791
Bsc4I	CCNNNNNNNGG	2 cut(s)	791, 2240
Bse1I	ACTGG	5 cut(s)	75, 106, 420, 1097, 1177
Bse3DI	GCAATG	4 cut(s)	674, 1116, 1615, 1762
BseAI	TCCGGA	1 cut(s)	791
BseBI	CCWGG	3 cut(s)	395, 1806, 1881
BseDI	CCNNGG	6 cut(s)	1081, 1673, 1880, 1925, 1966, 2052
BseGI	GGATG	7 cut(s)	670, 1197, 1441, 1636, 1712, 1825, 1861
BseLI	CCNNNNNNNGG	2 cut(s)	791, 2240
BseMI	GCAATG	4 cut(s)	674, 1116, 1615, 1762
BseNI	ACTGG	5 cut(s)	75, 106, 420, 1097, 1177
BseRI	GAGGAG	5 cut(s)	106, 361, 364, 717, 1435
BseXI	GCAGC	3 cut(s)	235, 438, 1093
BseYI	CCCAGC	1 cut(s)	51
BsgI	GTGCAG	1 cut(s)	2296
BshFI	GGCC	7 cut(s)	108, 543, 801, 1115, 1569, 1672, 1885
BshNI	GGYRCC	1 cut(s)	2023
BsiHKAI	GWGCWC	3 cut(s)	83, 225, 1236
BsiSI	CCGG	3 cut(s)	239, 294, 792
BsiWI	CGTACG	1 cut(s)	1561
BslFI	GGGAC	1 cut(s)	1850
BslI	CCNNNNNNNGG	2 cut(s)	791, 2240
BsmAI	GTCTC	6 cut(s)	63, 497, 996, 1204, 1404, 1500
BsmFI	GGGAC	1 cut(s)	1850
BsmI	GAATGC	1 cut(s)	511
BsnI	GGCC	7 cut(s)	108, 543, 801, 1115, 1569, 1672, 1885
Bso31I	GGTCTC	1 cut(s)	497
Bsp1286I	GDGCHC	3 cut(s)	83, 225, 1236
Bsp13I	TCCGGA	1 cut(s)	791
Bsp1720I	GCTNAGC	1 cut(s)	2034
BspACI	CCGC	5 cut(s)	758, 1681, 1823, 2083, 2301
BspANI	GGCC	7 cut(s)	108, 543, 801, 1115, 1569, 1672, 1885
BspEI	TCCGGA	1 cut(s)	791
BspHI	TCATGA	2 cut(s)	1416, 1479
BspLI	GGNNCC	5 cut(s)	107, 198, 1679, 1827, 2025
BspMAI	CTGCAG	4 cut(s)	889, 1526, 1621, 2118
BspMI	ACCTGC	3 cut(s)	842, 878, 1054
BspOI	GCTAGC	1 cut(s)	1542
BspPI	GGATC	6 cut(s)	191, 204, 783, 1088, 1568, 1796
BspT107I	GGYRCC	1 cut(s)	2023
BspTNI	GGTCTC	1 cut(s)	497
BsrBI	CCGCTC	1 cut(s)	1683
BsrDI	GCAATG	4 cut(s)	674, 1116, 1615, 1762
BsrI	ACTGG	5 cut(s)	75, 106, 420, 1097, 1177
BssECI	CCNNGG	6 cut(s)	1081, 1673, 1880, 1925, 1966, 2052
BssNI	GRCGYC	2 cut(s)	47, 2024
BssT1I	CCWWGG	4 cut(s)	1081, 1673, 1925, 2052
Bst2UI	CCWGG	3 cut(s)	395, 1806, 1881
Bst4CI	ACNGT	8 cut(s)	32, 508, 916, 1078, 1198, 1841, 2002, 2274
Bst6I	CTCTTC	3 cut(s)	2, 603, 1272
BstACI	GRCGYC	2 cut(s)	47, 2024
BstAPI	GCANNNNNTGC	1 cut(s)	893
BstBAI	YACGTR	3 cut(s)	1561, 1565, 2146
BstC8I	GCNNGC	7 cut(s)	409, 413, 665, 696, 849, 1540, 1993
BstF5I	GGATG	7 cut(s)	670, 1197, 1441, 1636, 1712, 1825, 1861
BstH2I	RGCGCY	2 cut(s)	1258, 2027
BstHHI	GCGC	2 cut(s)	1257, 2026
BstMAI	GTCTC	6 cut(s)	63, 497, 996, 1204, 1404, 1500
BstMWI	GCNNNNNNNGC	6 cut(s)	248, 766, 893, 1112, 2030, 2089
BstNI	CCWGG	3 cut(s)	395, 1806, 1881
BstNSI	RCATGY	2 cut(s)	451, 1396
BstSCI	CCNGG	3 cut(s)	393, 1804, 1879
BstSFI	CTRYAG	5 cut(s)	885, 1140, 1522, 1617, 2114
BstSNI	TACGTA	1 cut(s)	1561
BstV1I	GCAGC	3 cut(s)	235, 438, 1093
BstV2I	GAAGAC	3 cut(s)	121, 280, 577
BstX2I	RGATCY	4 cut(s)	196, 1093, 1573, 1742
BstYI	RGATCY	4 cut(s)	196, 1093, 1573, 1742
BsuI	GTATCC	1 cut(s)	1507
BsuRI	GGCC	7 cut(s)	108, 543, 801, 1115, 1569, 1672, 1885
BtrI	CACGTC	2 cut(s)	797, 2314
BtsCI	GGATG	7 cut(s)	670, 1197, 1441, 1636, 1712, 1825, 1861
BtsI	GCAGTG	2 cut(s)	653, 2135
BtsIMutI	CAGTG	7 cut(s)	189, 626, 653, 1846, 2135, 2175, 2279
BveI	ACCTGC	3 cut(s)	842, 878, 1054
Cac8I	GCNNGC	7 cut(s)	409, 413, 665, 696, 849, 1540, 1993
CaiI	CAGNNNCTG	1 cut(s)	1103
CciI	TCATGA	2 cut(s)	1416, 1479
CfoI	GCGC	2 cut(s)	1257, 2026
Cfr13I	GGNCC	5 cut(s)	106, 1262, 1677, 1808, 1883
CseI	GACGC	1 cut(s)	55
Csp6I	GTAC	4 cut(s)	481, 1315, 1558, 1562
CviQI	GTAC	4 cut(s)	481, 1315, 1558, 1562
DinI	GGCGCC	1 cut(s)	2025
DraI	TTTAAA	1 cut(s)	1347
DrdI	GACNNNNNNGTC	1 cut(s)	2286
DseDI	GACNNNNNNGTC	1 cut(s)	2286
EaeI	YGGCCR	3 cut(s)	799, 1113, 1670
Eam1104I	CTCTTC	3 cut(s)	2, 603, 1272
EarI	CTCTTC	3 cut(s)	2, 603, 1272
EciI	GGCGGA	1 cut(s)	747
Ecl136II	GAGCTC	1 cut(s)	1234
Eco105I	TACGTA	1 cut(s)	1561
Eco130I	CCWWGG	4 cut(s)	1081, 1673, 1925, 2052
Eco24I	GRGCYC	1 cut(s)	1236
Eco31I	GGTCTC	1 cut(s)	497
Eco47I	GGWCC	3 cut(s)	1262, 1677, 1808
Eco47III	AGCGCT	1 cut(s)	1256
Eco53kI	GAGCTC	1 cut(s)	1234
Eco57I	CTGAAG	1 cut(s)	687
EcoICRI	GAGCTC	1 cut(s)	1234
EcoO109I	RGGNCCY	1 cut(s)	1677
EcoRII	CCWGG	3 cut(s)	393, 1804, 1879
EcoT14I	CCWWGG	4 cut(s)	1081, 1673, 1925, 2052
EcoT38I	GRGCYC	1 cut(s)	1236
EgeI	GGCGCC	1 cut(s)	2025
EheI	GGCGCC	1 cut(s)	2025
ErhI	CCWWGG	4 cut(s)	1081, 1673, 1925, 2052
FaqI	GGGAC	1 cut(s)	1850
FauI	CCCGC	2 cut(s)	1688, 2308
FbaI	TGATCA	1 cut(s)	640
Fnu4HI	GCNGC	3 cut(s)	249, 452, 1107
FokI	GGATG	7 cut(s)	657, 1204, 1448, 1623, 1719, 1832, 1868
FriOI	GRGCYC	1 cut(s)	1236
Fsp4HI	GCNGC	3 cut(s)	249, 452, 1107
FspBI	CTAG	7 cut(s)	600, 897, 1455, 1539, 1571, 1926, 2066
GlaI	GCGC	2 cut(s)	1256, 2025
GluI	GCNGC	3 cut(s)	249, 452, 1107
GsaI	CCCAGC	1 cut(s)	55
GsuI	CTGGAG	2 cut(s)	273, 1723
HaeII	RGCGCY	2 cut(s)	1258, 2027
HaeIII	GGCC	7 cut(s)	108, 543, 801, 1115, 1569, 1672, 1885
HapII	CCGG	3 cut(s)	239, 294, 792
HgaI	GACGC	1 cut(s)	55
HhaI	GCGC	2 cut(s)	1257, 2026
Hin1I	GRCGYC	2 cut(s)	47, 2024
Hin6I	GCGC	2 cut(s)	1255, 2024
HinP1I	GCGC	2 cut(s)	1255, 2024
HincII	GTYRAC	3 cut(s)	919, 1390, 1656
HindII	GTYRAC	3 cut(s)	919, 1390, 1656
HindIII	AAGCTT	2 cut(s)	413, 845
HinfI	GANTC	8 cut(s)	172, 588, 754, 942, 1165, 1441, 1472, 1483
HpaII	CCGG	3 cut(s)	239, 294, 792
HphI	GGTGA	3 cut(s)	283, 496, 971
Hpy166II	GTNNAC	6 cut(s)	919, 992, 1390, 1656, 1844, 2126
Hpy8I	GTNNAC	6 cut(s)	919, 992, 1390, 1656, 1844, 2126
Hpy99I	CGWCG	2 cut(s)	49, 830
HpyAV	CCTTC	7 cut(s)	226, 554, 1010, 1355, 1596, 1948, 2306
HpyCH4III	ACNGT	8 cut(s)	32, 508, 916, 1078, 1198, 1841, 2002, 2274
HpyCH4IV	ACGT	9 cut(s)	574, 796, 825, 881, 1356, 1560, 1564, 2145, 2313
HpyF10VI	GCNNNNNNNGC	6 cut(s)	248, 766, 893, 1112, 2030, 2089
HpySE526I	ACGT	9 cut(s)	574, 796, 825, 881, 1356, 1560, 1564, 2145, 2313
Hsp92I	GRCGYC	2 cut(s)	47, 2024
HspAI	GCGC	2 cut(s)	1255, 2024
KasI	GGCGCC	1 cut(s)	2023
Kpn2I	TCCGGA	1 cut(s)	791
Ksp22I	TGATCA	1 cut(s)	640
LmnI	GCTCC	5 cut(s)	86, 1231, 1605, 2067, 2297
Lsp1109I	GCAGC	3 cut(s)	235, 438, 1093
LweI	GCATC	4 cut(s)	343, 463, 1723, 1810
MaeI	CTAG	7 cut(s)	600, 897, 1455, 1539, 1571, 1926, 2066
MaeII	ACGT	9 cut(s)	574, 796, 825, 881, 1356, 1560, 1564, 2145, 2313
MaeIII	GTNAC	3 cut(s)	40, 1612, 1948
MbiI	CCGCTC	1 cut(s)	1683
MfeI	CAATTG	3 cut(s)	123, 717, 1032
MflI	RGATCY	4 cut(s)	196, 1093, 1573, 1742
MhlI	GDGCHC	3 cut(s)	83, 225, 1236
MlsI	TGGCCA	2 cut(s)	1115, 1672
MluNI	TGGCCA	2 cut(s)	1115, 1672
Mly113I	GGCGCC	1 cut(s)	2024
MlyI	GAGTC	4 cut(s)	166, 763, 1466, 1477
MmeI	TCCRAC	2 cut(s)	200, 288
Mox20I	TGGCCA	2 cut(s)	1115, 1672
MroI	TCCGGA	1 cut(s)	791
MroXI	GAANNNNTTC	2 cut(s)	1632, 1920
MscI	TGGCCA	2 cut(s)	1115, 1672
MslI	CAYNNNNRTG	2 cut(s)	459, 2076
Msp20I	TGGCCA	2 cut(s)	1115, 1672
MspI	CCGG	3 cut(s)	239, 294, 792
MspR9I	CCNGG	3 cut(s)	395, 1806, 1881
MunI	CAATTG	3 cut(s)	123, 717, 1032
Mva1269I	GAATGC	1 cut(s)	511
MvaI	CCWGG	3 cut(s)	395, 1806, 1881
MwoI	GCNNNNNNNGC	6 cut(s)	248, 766, 893, 1112, 2030, 2089
NarI	GGCGCC	1 cut(s)	2024
NheI	GCTAGC	1 cut(s)	1538
NlaIV	GGNNCC	5 cut(s)	107, 198, 1679, 1827, 2025
NmeAIII	GCCGAG	1 cut(s)	572
NspI	RCATGY	2 cut(s)	451, 1396
PagI	TCATGA	2 cut(s)	1416, 1479
PciI	ACATGT	1 cut(s)	1392
PcsI	WCGNNNNNNNCGW	2 cut(s)	571, 793
PctI	GAATGC	1 cut(s)	511
PdmI	GAANNNNTTC	2 cut(s)	1632, 1920
PfeI	GAWTC	4 cut(s)	588, 942, 1165, 1441
Pfl23II	CGTACG	1 cut(s)	1561
PkrI	GCNGC	3 cut(s)	250, 453, 1108
PleI	GAGTC	4 cut(s)	166, 762, 1466, 1477
PluTI	GGCGCC	1 cut(s)	2027
PpsI	GAGTC	4 cut(s)	166, 762, 1466, 1477
Ppu21I	YACGTR	3 cut(s)	1561, 1565, 2146
PpuMI	RGGWCCY	1 cut(s)	1677
PscI	ACATGT	1 cut(s)	1392
PshBI	ATTAAT	3 cut(s)	710, 737, 972
PsiI	TTATAA	1 cut(s)	557
Psp124BI	GAGCTC	1 cut(s)	1236
Psp5II	RGGWCCY	1 cut(s)	1677
Psp6I	CCWGG	3 cut(s)	393, 1804, 1879
PspFI	CCCAGC	1 cut(s)	51
PspGI	CCWGG	3 cut(s)	393, 1804, 1879
PspLI	CGTACG	1 cut(s)	1561
PspN4I	GGNNCC	5 cut(s)	107, 198, 1679, 1827, 2025
PspPI	GGNCC	5 cut(s)	106, 1262, 1677, 1808, 1883
PspPPI	RGGWCCY	1 cut(s)	1677
PstI	CTGCAG	4 cut(s)	889, 1526, 1621, 2118
PstNI	CAGNNNCTG	1 cut(s)	1103
PsuI	RGATCY	4 cut(s)	196, 1093, 1573, 1742
RsaI	GTAC	4 cut(s)	482, 1316, 1559, 1563
RsaNI	GTAC	4 cut(s)	481, 1315, 1558, 1562
RseI	CAYNNNNRTG	2 cut(s)	459, 2076
SacI	GAGCTC	1 cut(s)	1236
SatI	GCNGC	3 cut(s)	249, 452, 1107
Sau96I	GGNCC	5 cut(s)	106, 1262, 1677, 1808, 1883
SchI	GAGTC	4 cut(s)	166, 763, 1466, 1477
ScrFI	CCNGG	3 cut(s)	395, 1806, 1881
SduI	GDGCHC	3 cut(s)	83, 225, 1236
SfaNI	GCATC	4 cut(s)	343, 463, 1723, 1810
SfcI	CTRYAG	5 cut(s)	885, 1140, 1522, 1617, 2114
SfoI	GGCGCC	1 cut(s)	2025
SinI	GGWCC	3 cut(s)	1262, 1677, 1808
SmiMI	CAYNNNNRTG	2 cut(s)	459, 2076
SmlI	CTYRAG	1 cut(s)	2013
SmoI	CTYRAG	1 cut(s)	2013
SnaBI	TACGTA	1 cut(s)	1561
SsiI	CCGC	5 cut(s)	758, 1681, 1823, 2083, 2301
SspDI	GGCGCC	1 cut(s)	2023
SspMI	CTAG	7 cut(s)	600, 897, 1455, 1539, 1571, 1926, 2066
SstI	GAGCTC	1 cut(s)	1236
StyD4I	CCNGG	3 cut(s)	393, 1804, 1879
StyI	CCWWGG	4 cut(s)	1081, 1673, 1925, 2052
TaaI	ACNGT	8 cut(s)	32, 508, 916, 1078, 1198, 1841, 2002, 2274
TaiI	ACGT	9 cut(s)	577, 799, 828, 884, 1359, 1563, 1567, 2148, 2316
TaqI	TCGA	5 cut(s)	219, 586, 1470, 1492, 1691
TatI	WGTACW	1 cut(s)	1314
TfiI	GAWTC	4 cut(s)	588, 942, 1165, 1441
TscAI	CASTG	7 cut(s)	196, 626, 653, 1846, 2142, 2175, 2279
TseI	GCWGC	3 cut(s)	248, 451, 1106
TspDTI	ATGAA	7 cut(s)	273, 1053, 1338, 1625, 1947, 2060, 2095
TspGWI	ACGGA	1 cut(s)	1448
TspRI	CASTG	7 cut(s)	196, 626, 653, 1846, 2142, 2175, 2279
VpaK11BI	GGWCC	3 cut(s)	1262, 1677, 1808
VspI	ATTAAT	3 cut(s)	710, 737, 972
XapI	RAATTY	2 cut(s)	1020, 1295
XceI	RCATGY	2 cut(s)	451, 1396
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1256, 1812, 2207
XmaJI	CCTAGG	1 cut(s)	1925
XmnI	GAANNNNTTC	2 cut(s)	1632, 1920
XspI	CTAG	7 cut(s)	600, 897, 1455, 1539, 1571, 1926, 2066

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.