Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0005278

Physical Location & Seq

Genomic Coordinates Forward (+)

66285077 .. 66295657

Gene Structure 10581 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00387670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2301 bp

ATGCAGAAGAGTGGGAGTGTTTCCAAGAATAGTTTGAGAGTGACAACCCCTCAGCAGTCCCTTAGGCGACTGGGCTTGTGCTCCCAAATAGCTACTGCTACTGGTGGCCAGCACTCCTCCCCAATTGTCTTCCCTGAGAAACAGAAACGACACAAGATCAAGGCTTCTAAGACTCCTACTACTCCTGCCTCTGCTGCTGATGATCCCAATTCTCTCAAGACCTTCGAGCACAGGATTGACATTCCAGCTTCTGCTGCTGGAGATGAAAAGTCTGATTTGTTGGGTTATGCCGTCTTCTCTGGAAAGTTAGTTTTGGACAAGAGTAAAACCACTCTCGTAAACACCACTTCTACTGATCCACAACAACAAACCTCCTCCTCTACCAACCAGGAAGCTGTTGATGCTAAGCTTACAAGCAAGGCCTTAATTTGGGGTTCTCACATGCTGCATCTGGATGATGTTATCTCGGTTTCATACAATGTTGGGCTCAGACATTTTACCGTGCATTCTTATCCCTTAAAAAATGGTTCTTTTGGCCTTTCTTGTTTCATGAAACCTCGAAGAAGTCGCAAGGACTTTCGATTCTTGGCTTCTAGCATAGAAGACGCCGTTCAATGGGTTGGTGGGTTTGCTGATCAGCAGTGTTATGTGAATTGCTTGCCTCATCCATTACTATCTTCTAAGAAGCAGGCTTCTTCAGAATTACTTCCCATTGATACTCCTCCTGAATTAATTTTCAAATGTAAGAGCCCACCAAAAATGCTTGTCATATTGAATCCACAATCTGGACGTGGCCGTTCGAGTAAGGTTTTTCACAGCATCGTTGAACCTATATTTAAGCTTGCAGGTTTCAAAGTGGAGGTAGTCAAAACAACATCGGCAGGTCATGCTAAGAAACTTGCATCTAGTGTTGACATTAGCAGTTGTCCTGATGGAATCATATGTGTTGGGGGCGATGGAATTATTAATGAGGTTCTAAATGGTTTACTTAGTAGAGACAACCAAAAAGAAGGAATTTCCATACCAATTGGAATTATACCTGCTGGTTCCGATAATTCCTTAGTTTGGACGGTTCTTGGTGTTAGGGATCCAGTTTCTGCTGCAATGGCTATTGTCAAGGGTGGTCTTACTGCTACAGATGTTTTTGCTGTTGAGTGGATTCAGACGGGTGTTATCCACTTTGGGATGACAGTCTCTTATTATGGTTTTGTGAGTGATGTGTTGGAGCTGTCTGAGAAATATCAGAAGCGCTTTGGTCCTCTGCGTTATTTTGTTGCTGGATTTCTTAAATTCTTATGCCTGCCGAAGTATAGCTATGAAGTTGAGTACCTTCCAGCATCAAAAGAGGATCTAGAAGGAAAACTCTCAGCTGAAATTGATGTGTCAGACTTGTACACAGACATTATGAGGAGATCAAATACCGATGGTATTCCAAGAGCTTCTAGTTTATCAAGTATTGACTCCATTATGACTCCTAGTCGAATGTCTGGAGGTGACTTGGATACAACCTGCAGTAGCACTCATGCTAGCATTGAACCATCTGAGTACGTGCGTGGCCTAGATCCTAAAGCAAAAAGACTGTCAATTGGAAGGACCAATATAACTGCAGAACCGGAAGTTATTCATCCTCAGCTGCCCCTGTCAACAACACCAAACTGGCCAAGGACCCGGTCAAAGTCAAGGACAGATAGAGGATGGACTGGATTGACAGCCACACATGATACCTCTAGATCTTCTTGGGGAAACACTGGAACAAATGATAAAGAGGATATATCATCCACGCTATCCGATCCAGGCCCAATTTGGGATGCTGAGCCAAAGTGGGATACTGAACCTAATTGGGATGTGGAAAATCCTATTGAATTGCCAGGGCCATCAGATGACGTAGAAGCAGGAACTAAGGAAGTAGTGCCCAGGTATGAAGATAAATGGGTTGTGACCAAGGGTCAGTTGCTTGGCATTCTTGTTTGCAATCATGCATGCAGAACTGTTCAGAGTTCCCAAGTGGTGGCACCAAAAGCTGAGCATGATGACAACACCTTGGATTTGCTCCTAGTTCATGGAAGTGGGCGATTAAGGCTGTTGAGGTTTTTCATGCTTCTGCAGATGGGTAGACACCTTTCGCTGCCATACGTGGAAAATGTCAAGGTAAAGTCGGTGAAGATCAAGGCATCAGGAAAGCACACCCATAACGGTTGTGGCATTGATGGGGAGCTTTTTCCACTTAATGGGCAAGTCATTTCTTCGTTGCTTCCAGAACAGTGCAGACTTATTGGTCGCTCCTATAACCATCACGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006629 GO:0006643 GO:0006665 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0017050 GO:0030148 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0046467 GO:0071704 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAGK_cat

Protein Analysis

766

Amino Acids

83.69

Weight (kDa)

8.39

Isoelectric Point (pI)

36.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	253 - 383	1.4e-29	Diacylglycerol kinase catalytic domain
YegS_C	PF19279	667 - 744	1.5e-10	YegS C-terminal NAD kinase beta sandwich-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005278)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23450 AT5G23450 AT5G23450 AT5G23450 AT5G23450
fragaria_vesca	FvH4_4g37380 FvH4_4g37380 FvH4_4g37380
malus_domestica	MD13G1000100.v1.1 MD16G1005700.v1.1 MD16G1005800.v1.1
prunus_persica	Prupe.1G584800_v2.0.a1
pyrus_communis	pycom13g00010 pycom2749g00100 pycom2749g00120
rosa_chinensis	RchiOBHm_Chr4g0447291
rosa_laevigata	RLG00000005589
rosa_multiflora	Rmu_co8061930.1_g000001 Rmu_sc0017601.1_g000001 Rmu_ssc0000396.1_g000006
rosa_roxburghii	Rroxscaffold_5G00387670
rosa_rugosa	Rorug04G0379600 Rorug04G0379600 Rorug04G0379700
rosa_samantha	Rh4AG440900 Rh4BG450000 Rh4CG467100 Rh4DG448400
rosa_wichuraiana	Rw4G037730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2274
Acc36I	ACCTGC	4 cut(s)	836, 872, 1048, 1517
AccB1I	GGYRCC	1 cut(s)	2011
AccB7I	CCANNNNNTGG	3 cut(s)	785, 2008, 2228
AccI	GTMKAC	1 cut(s)	2113
AclWI	GGATC	7 cut(s)	197, 350, 1082, 1095, 1356, 1556, 1784
AcoI	YGGCCR	3 cut(s)	106, 793, 1658
AcsI	RAATTY	2 cut(s)	1014, 1289
AcuI	CTGAAG	1 cut(s)	681
AcyI	GRCGYC	1 cut(s)	606
AfaI	GTAC	3 cut(s)	1328, 1394, 1547
AfeI	AGCGCT	1 cut(s)	1250
AfiI	CCNNNNNNNGG	7 cut(s)	429, 615, 785, 1065, 1804, 2008, 2228
AgsI	TTSAA	7 cut(s)	614, 739, 775, 827, 853, 1535, 1862
AhdI	GACNNNNNGTC	2 cut(s)	1476, 1944
AjiI	CACGTC	2 cut(s)	791, 2296
AjnI	CCWGG	4 cut(s)	387, 1792, 1867, 1913
Alw21I	GWGCWC	2 cut(s)	83, 231
Alw26I	GTCTC	2 cut(s)	990, 1198
AlwI	GGATC	7 cut(s)	197, 350, 1082, 1095, 1356, 1556, 1784
AlwNI	CAGNNNCTG	2 cut(s)	251, 1097
Aor51HI	AGCGCT	1 cut(s)	1250
AoxI	GGCC	8 cut(s)	106, 420, 535, 793, 1555, 1658, 1795, 1871
ApeKI	GCWGC	6 cut(s)	194, 254, 445, 1100, 1633, 2125
ApoI	RAATTY	2 cut(s)	1014, 1289
ArsI	GACNNNNNNTTYG	2 cut(s)	1565, 1597
AseI	ATTAAT	2 cut(s)	731, 966
Asp700I	GAANNNNTTC	2 cut(s)	705, 1620
AspLEI	GCGC	1 cut(s)	1251
AspS9I	GGNCC	5 cut(s)	1256, 1593, 1665, 1796, 1871
AsuC2I	CCSGG	1 cut(s)	1669
AsuHPI	GGTGA	2 cut(s)	1505, 2170
AsuNHI	GCTAGC	1 cut(s)	1526
AvaII	GGWCC	3 cut(s)	1256, 1593, 1665
AxyI	CCTNAGG	1 cut(s)	62
BaeGI	GKGCMC	1 cut(s)	1914
BalI	TGGCCA	2 cut(s)	108, 1660
BamHI	GGATCC	1 cut(s)	1087
BanI	GGYRCC	1 cut(s)	2011
BanII	GRGCYC	2 cut(s)	489, 752
BbsI	GAAGAC	3 cut(s)	121, 286, 609
Bbv12I	GWGCWC	2 cut(s)	83, 231
BbvCI	CCTCAGC	2 cut(s)	51, 1629
BbvI	GCAGC	6 cut(s)	181, 241, 432, 1087, 1620, 2112
BccI	CCATC	9 cut(s)	926, 950, 1418, 1546, 1689, 1882, 2101, 2201, 2298
BceAI	ACGGC	3 cut(s)	275, 593, 780
BciT130I	CCWGG	4 cut(s)	389, 1794, 1869, 1915
BciVI	GTATCC	2 cut(s)	1495, 1819
BclI	TGATCA	1 cut(s)	634
BcnI	CCSGG	1 cut(s)	1669
BcoDI	GTCTC	2 cut(s)	990, 1198
BfaI	CTAG	9 cut(s)	594, 906, 1352, 1443, 1476, 1527, 1559, 1728, 2054
BfmI	CTRYAG	4 cut(s)	1134, 1510, 1605, 2102
BfoI	RGCGCY	1 cut(s)	1252
BfuAI	ACCTGC	4 cut(s)	836, 872, 1048, 1517
BfuI	GTATCC	2 cut(s)	1495, 1819
BglII	AGATCT	1 cut(s)	1730
BisI	GCNGC	6 cut(s)	195, 255, 446, 1101, 1634, 2126
BlpI	GCTNAGC	3 cut(s)	405, 1812, 2022
BlsI	GCNGC	6 cut(s)	196, 256, 447, 1102, 1635, 2127
Bme1390I	CCNGG	5 cut(s)	389, 1669, 1794, 1869, 1915
Bme18I	GGWCC	3 cut(s)	1256, 1593, 1665
BmeRI	GACNNNNNGTC	2 cut(s)	1476, 1944
BmgBI	CACGTC	2 cut(s)	791, 2296
BmgT120I	GGNCC	5 cut(s)	1256, 1593, 1665, 1796, 1871
BmiI	GGNNCC	4 cut(s)	1048, 1089, 1667, 2013
BmrFI	CCNGG	5 cut(s)	389, 1669, 1794, 1869, 1915
BmrI	ACTGGG	1 cut(s)	80
BmsI	GCATC	7 cut(s)	391, 457, 828, 911, 1346, 1798, 2180
BmtI	GCTAGC	1 cut(s)	1530
BmuI	ACTGGG	1 cut(s)	80
BpiI	GAAGAC	3 cut(s)	121, 286, 609
BpmI	CTGGAG	2 cut(s)	279, 1509
Bpu10I	CCTNAGC	2 cut(s)	51, 1629
Bpu1102I	GCTNAGC	3 cut(s)	405, 1812, 2022
BpuEI	CTTGAG	1 cut(s)	200
BpuMI	CCSGG	1 cut(s)	1669
BsaAI	YACGTR	2 cut(s)	1549, 2134
BsaHI	GRCGYC	1 cut(s)	606
BsaJI	CCNNGG	5 cut(s)	1661, 1868, 1913, 1941, 2040
BsaWI	WCCGGW	1 cut(s)	1612
Bsc4I	CCNNNNNNNGG	7 cut(s)	429, 615, 785, 1065, 1804, 2008, 2228
Bse1I	ACTGG	6 cut(s)	75, 106, 1091, 1661, 1705, 1753
Bse21I	CCTNAGG	1 cut(s)	62
Bse3DI	GCAATG	1 cut(s)	1110
BseBI	CCWGG	4 cut(s)	389, 1794, 1869, 1915
BseDI	CCNNGG	5 cut(s)	1661, 1868, 1913, 1941, 2040
BseGI	GGATG	8 cut(s)	460, 664, 1191, 1624, 1700, 1775, 1813, 1849
BseLI	CCNNNNNNNGG	7 cut(s)	429, 615, 785, 1065, 1804, 2008, 2228
BseMI	GCAATG	1 cut(s)	1110
BseMII	CTCAG	9 cut(s)	65, 126, 502, 1224, 1380, 1533, 1643, 1803, 2013
BseNI	ACTGG	6 cut(s)	75, 106, 1091, 1661, 1705, 1753
BseRI	GAGGAG	5 cut(s)	106, 364, 367, 711, 1423
BseSI	GKGCMC	1 cut(s)	1914
BseXI	GCAGC	6 cut(s)	181, 241, 432, 1087, 1620, 2112
BsgI	GTGCAG	1 cut(s)	2284
BshFI	GGCC	8 cut(s)	108, 422, 537, 795, 1557, 1660, 1797, 1873
BshNI	GGYRCC	1 cut(s)	2011
BsiHKAI	GWGCWC	2 cut(s)	83, 231
BsiSI	CCGG	2 cut(s)	1613, 1669
BslFI	GGGAC	1 cut(s)	43
BslI	CCNNNNNNNGG	7 cut(s)	429, 615, 785, 1065, 1804, 2008, 2228
BsmAI	GTCTC	2 cut(s)	990, 1198
BsmFI	GGGAC	1 cut(s)	43
BsmI	GAATGC	2 cut(s)	505, 1959
BsnI	GGCC	8 cut(s)	108, 422, 537, 795, 1557, 1660, 1797, 1873
Bsp1286I	GDGCHC	5 cut(s)	83, 231, 489, 752, 1914
Bsp1407I	TGTACA	1 cut(s)	1392
Bsp1720I	GCTNAGC	3 cut(s)	405, 1812, 2022
BspANI	GGCC	8 cut(s)	108, 422, 537, 795, 1557, 1660, 1797, 1873
BspCNI	CTCAG	9 cut(s)	64, 127, 501, 1225, 1379, 1534, 1642, 1804, 2014
BspHI	TCATGA	1 cut(s)	549
BspLI	GGNNCC	4 cut(s)	1048, 1089, 1667, 2013
BspMAI	CTGCAG	3 cut(s)	1514, 1609, 2106
BspMI	ACCTGC	4 cut(s)	836, 872, 1048, 1517
BspOI	GCTAGC	1 cut(s)	1530
BspPI	GGATC	7 cut(s)	197, 350, 1082, 1095, 1356, 1556, 1784
BspT107I	GGYRCC	1 cut(s)	2011
BsrDI	GCAATG	1 cut(s)	1110
BsrGI	TGTACA	1 cut(s)	1392
BsrI	ACTGG	6 cut(s)	75, 106, 1091, 1661, 1705, 1753
BssECI	CCNNGG	5 cut(s)	1661, 1868, 1913, 1941, 2040
BssNI	GRCGYC	1 cut(s)	606
BssT1I	CCWWGG	3 cut(s)	1661, 1941, 2040
Bst2UI	CCWGG	4 cut(s)	389, 1794, 1869, 1915
Bst4CI	ACNGT	7 cut(s)	502, 1072, 1192, 1581, 1990, 2195, 2262
Bst6I	CTCTTC	1 cut(s)	2
BstACI	GRCGYC	1 cut(s)	606
BstAPI	GCANNNNNTGC	1 cut(s)	887
BstAUI	TGTACA	1 cut(s)	1392
BstBAI	YACGTR	2 cut(s)	1549, 2134
BstC8I	GCNNGC	7 cut(s)	110, 659, 690, 843, 1301, 1528, 1981
BstF5I	GGATG	8 cut(s)	460, 664, 1191, 1624, 1700, 1775, 1813, 1849
BstH2I	RGCGCY	1 cut(s)	1252
BstHHI	GCGC	1 cut(s)	1251
BstMAI	GTCTC	2 cut(s)	990, 1198
BstMWI	GCNNNNNNNGC	7 cut(s)	194, 254, 401, 887, 1106, 2018, 2077
BstNI	CCWGG	4 cut(s)	389, 1794, 1869, 1915
BstNSI	RCATGY	2 cut(s)	445, 1983
BstSCI	CCNGG	5 cut(s)	387, 1667, 1792, 1867, 1913
BstSFI	CTRYAG	4 cut(s)	1134, 1510, 1605, 2102
BstSLI	GKGCMC	1 cut(s)	1914
BstV1I	GCAGC	6 cut(s)	181, 241, 432, 1087, 1620, 2112
BstV2I	GAAGAC	3 cut(s)	121, 286, 609
BstX2I	RGATCY	4 cut(s)	1087, 1348, 1561, 1730
BstYI	RGATCY	4 cut(s)	1087, 1348, 1561, 1730
Bsu36I	CCTNAGG	1 cut(s)	62
BsuI	GTATCC	2 cut(s)	1495, 1819
BsuRI	GGCC	8 cut(s)	108, 422, 537, 795, 1557, 1660, 1797, 1873
BtgZI	GCGATG	1 cut(s)	969
BtrI	CACGTC	2 cut(s)	791, 2296
BtsCI	GGATG	8 cut(s)	460, 664, 1191, 1624, 1700, 1775, 1813, 1849
BtsI	GCAGTG	1 cut(s)	647
BtsIMutI	CAGTG	3 cut(s)	647, 1746, 2267
BveI	ACCTGC	4 cut(s)	836, 872, 1048, 1517
Cac8I	GCNNGC	7 cut(s)	110, 659, 690, 843, 1301, 1528, 1981
CaiI	CAGNNNCTG	2 cut(s)	251, 1097
CciI	TCATGA	1 cut(s)	549
CfoI	GCGC	1 cut(s)	1251
Cfr13I	GGNCC	5 cut(s)	1256, 1593, 1665, 1796, 1871
CseI	GACGC	1 cut(s)	614
Csp6I	GTAC	3 cut(s)	1327, 1393, 1546
CviQI	GTAC	3 cut(s)	1327, 1393, 1546
DrdI	GACNNNNNNGTC	1 cut(s)	2274
DriI	GACNNNNNGTC	2 cut(s)	1476, 1944
DseDI	GACNNNNNNGTC	1 cut(s)	2274
EaeI	YGGCCR	3 cut(s)	106, 793, 1658
Eam1104I	CTCTTC	1 cut(s)	2
Eam1105I	GACNNNNNGTC	2 cut(s)	1476, 1944
EarI	CTCTTC	1 cut(s)	2
Eco130I	CCWWGG	3 cut(s)	1661, 1941, 2040
Eco147I	AGGCCT	1 cut(s)	422
Eco24I	GRGCYC	2 cut(s)	489, 752
Eco47I	GGWCC	3 cut(s)	1256, 1593, 1665
Eco47III	AGCGCT	1 cut(s)	1250
Eco57I	CTGAAG	1 cut(s)	681
Eco81I	CCTNAGG	1 cut(s)	62
EcoO109I	RGGNCCY	1 cut(s)	1665
EcoRII	CCWGG	4 cut(s)	387, 1792, 1867, 1913
EcoT14I	CCWWGG	3 cut(s)	1661, 1941, 2040
EcoT22I	ATGCAT	1 cut(s)	1981
EcoT38I	GRGCYC	2 cut(s)	489, 752
ErhI	CCWWGG	3 cut(s)	1661, 1941, 2040
FaqI	GGGAC	1 cut(s)	43
FauNDI	CATATG	1 cut(s)	941
FbaI	TGATCA	1 cut(s)	634
FblI	GTMKAC	1 cut(s)	2113
Fnu4HI	GCNGC	6 cut(s)	195, 255, 446, 1101, 1634, 2126
FokI	GGATG	8 cut(s)	467, 651, 1198, 1611, 1707, 1762, 1820, 1856
FriOI	GRGCYC	2 cut(s)	489, 752
Fsp4HI	GCNGC	6 cut(s)	195, 255, 446, 1101, 1634, 2126
FspBI	CTAG	9 cut(s)	594, 906, 1352, 1443, 1476, 1527, 1559, 1728, 2054
GlaI	GCGC	1 cut(s)	1250
GluI	GCNGC	6 cut(s)	195, 255, 446, 1101, 1634, 2126
GsuI	CTGGAG	2 cut(s)	279, 1509
HaeII	RGCGCY	1 cut(s)	1252
HaeIII	GGCC	8 cut(s)	108, 422, 537, 795, 1557, 1660, 1797, 1873
HapII	CCGG	2 cut(s)	1613, 1669
HgaI	GACGC	1 cut(s)	614
HhaI	GCGC	1 cut(s)	1251
Hin1I	GRCGYC	1 cut(s)	606
Hin6I	GCGC	1 cut(s)	1249
HinP1I	GCGC	1 cut(s)	1249
HincII	GTYRAC	2 cut(s)	913, 1644
HindII	GTYRAC	2 cut(s)	913, 1644
HindIII	AAGCTT	2 cut(s)	407, 839
HinfI	GANTC	7 cut(s)	172, 582, 775, 936, 1159, 1460, 1471
HpaII	CCGG	2 cut(s)	1613, 1669
HphI	GGTGA	2 cut(s)	1505, 2170
Hpy166II	GTNNAC	6 cut(s)	340, 913, 986, 1395, 1644, 2114
Hpy8I	GTNNAC	6 cut(s)	340, 913, 986, 1395, 1644, 2114
HpyAV	CCTTC	5 cut(s)	232, 1004, 1340, 1349, 1584
HpyCH4III	ACNGT	7 cut(s)	502, 1072, 1192, 1581, 1990, 2195, 2262
HpyCH4IV	ACGT	5 cut(s)	790, 1548, 1884, 2133, 2295
HpyF10VI	GCNNNNNNNGC	7 cut(s)	194, 254, 401, 887, 1106, 2018, 2077
HpySE526I	ACGT	5 cut(s)	790, 1548, 1884, 2133, 2295
Hsp92I	GRCGYC	1 cut(s)	606
HspAI	GCGC	1 cut(s)	1249
Ksp22I	TGATCA	1 cut(s)	634
LmnI	GCTCC	5 cut(s)	86, 1225, 2055, 2212, 2285
Lsp1109I	GCAGC	6 cut(s)	181, 241, 432, 1087, 1620, 2112
LweI	GCATC	7 cut(s)	391, 457, 828, 911, 1346, 1798, 2180
MaeI	CTAG	9 cut(s)	594, 906, 1352, 1443, 1476, 1527, 1559, 1728, 2054
MaeII	ACGT	5 cut(s)	790, 1548, 1884, 2133, 2295
MaeIII	GTNAC	3 cut(s)	40, 1493, 1936
MfeI	CAATTG	3 cut(s)	123, 1026, 1584
MflI	RGATCY	4 cut(s)	1087, 1348, 1561, 1730
MhlI	GDGCHC	5 cut(s)	83, 231, 489, 752, 1914
MlsI	TGGCCA	2 cut(s)	108, 1660
MluNI	TGGCCA	2 cut(s)	108, 1660
MlyI	GAGTC	3 cut(s)	166, 1454, 1465
MmeI	TCCRAC	1 cut(s)	1203
Mox20I	TGGCCA	2 cut(s)	108, 1660
Mph1103I	ATGCAT	1 cut(s)	1981
MroXI	GAANNNNTTC	2 cut(s)	705, 1620
MscI	TGGCCA	2 cut(s)	108, 1660
MseI	TTAA	8 cut(s)	425, 518, 731, 837, 966, 1287, 2075, 2226
MslI	CAYNNNNRTG	2 cut(s)	453, 2064
Msp20I	TGGCCA	2 cut(s)	108, 1660
MspA1I	CMGCKG	2 cut(s)	1370, 1633
MspI	CCGG	2 cut(s)	1613, 1669
MspR9I	CCNGG	5 cut(s)	389, 1669, 1794, 1869, 1915
MunI	CAATTG	3 cut(s)	123, 1026, 1584
Mva1269I	GAATGC	2 cut(s)	505, 1959
MvaI	CCWGG	4 cut(s)	389, 1794, 1869, 1915
MwoI	GCNNNNNNNGC	7 cut(s)	194, 254, 401, 887, 1106, 2018, 2077
NciI	CCSGG	1 cut(s)	1669
NdeI	CATATG	1 cut(s)	941
NheI	GCTAGC	1 cut(s)	1526
NlaIV	GGNNCC	4 cut(s)	1048, 1089, 1667, 2013
NmuCI	GTSAC	3 cut(s)	40, 1493, 1936
NsiI	ATGCAT	1 cut(s)	1981
NspI	RCATGY	2 cut(s)	445, 1983
PaeI	GCATGC	1 cut(s)	1983
PagI	TCATGA	1 cut(s)	549
PceI	AGGCCT	1 cut(s)	422
PctI	GAATGC	2 cut(s)	505, 1959
PdmI	GAANNNNTTC	2 cut(s)	705, 1620
PfeI	GAWTC	4 cut(s)	582, 775, 936, 1159
PflFI	GACNNNGTC	1 cut(s)	1669
PflMI	CCANNNNNTGG	3 cut(s)	785, 2008, 2228
PkrI	GCNGC	6 cut(s)	196, 256, 447, 1102, 1635, 2127
PleI	GAGTC	3 cut(s)	166, 1454, 1465
PpsI	GAGTC	3 cut(s)	166, 1454, 1465
Ppu21I	YACGTR	2 cut(s)	1549, 2134
PpuMI	RGGWCCY	1 cut(s)	1665
PshBI	ATTAAT	2 cut(s)	731, 966
Psp5II	RGGWCCY	1 cut(s)	1665
Psp6I	CCWGG	4 cut(s)	387, 1792, 1867, 1913
PspGI	CCWGG	4 cut(s)	387, 1792, 1867, 1913
PspN4I	GGNNCC	4 cut(s)	1048, 1089, 1667, 2013
PspPI	GGNCC	5 cut(s)	1256, 1593, 1665, 1796, 1871
PspPPI	RGGWCCY	1 cut(s)	1665
PstI	CTGCAG	3 cut(s)	1514, 1609, 2106
PstNI	CAGNNNCTG	2 cut(s)	251, 1097
PsuI	RGATCY	4 cut(s)	1087, 1348, 1561, 1730
PsyI	GACNNNGTC	1 cut(s)	1669
PvuII	CAGCTG	2 cut(s)	1370, 1633
RsaI	GTAC	3 cut(s)	1328, 1394, 1547
RsaNI	GTAC	3 cut(s)	1327, 1393, 1546
RseI	CAYNNNNRTG	2 cut(s)	453, 2064
SaqAI	TTAA	8 cut(s)	425, 518, 731, 837, 966, 1287, 2075, 2226
SatI	GCNGC	6 cut(s)	195, 255, 446, 1101, 1634, 2126
Sau96I	GGNCC	5 cut(s)	1256, 1593, 1665, 1796, 1871
SchI	GAGTC	3 cut(s)	166, 1454, 1465
ScrFI	CCNGG	5 cut(s)	389, 1669, 1794, 1869, 1915
SduI	GDGCHC	5 cut(s)	83, 231, 489, 752, 1914
SfaNI	GCATC	7 cut(s)	391, 457, 828, 911, 1346, 1798, 2180
SfcI	CTRYAG	4 cut(s)	1134, 1510, 1605, 2102
SinI	GGWCC	3 cut(s)	1256, 1593, 1665
SmiMI	CAYNNNNRTG	2 cut(s)	453, 2064
SmlI	CTYRAG	1 cut(s)	215
SmoI	CTYRAG	1 cut(s)	215
SphI	GCATGC	1 cut(s)	1983
SseBI	AGGCCT	1 cut(s)	422
SspMI	CTAG	9 cut(s)	594, 906, 1352, 1443, 1476, 1527, 1559, 1728, 2054
StuI	AGGCCT	1 cut(s)	422
StyD4I	CCNGG	5 cut(s)	387, 1667, 1792, 1867, 1913
StyI	CCWWGG	3 cut(s)	1661, 1941, 2040
TaaI	ACNGT	7 cut(s)	502, 1072, 1192, 1581, 1990, 2195, 2262
TaiI	ACGT	5 cut(s)	793, 1551, 1887, 2136, 2298
TaqI	TCGA	5 cut(s)	225, 559, 580, 800, 1480
TatI	WGTACW	1 cut(s)	1392
TfiI	GAWTC	4 cut(s)	582, 775, 936, 1159
Tru1I	TTAA	8 cut(s)	425, 518, 731, 837, 966, 1287, 2075, 2226
Tru9I	TTAA	8 cut(s)	425, 518, 731, 837, 966, 1287, 2075, 2226
TscAI	CASTG	3 cut(s)	647, 1753, 2267
TseFI	GTSAC	3 cut(s)	40, 1493, 1936
TseI	GCWGC	6 cut(s)	194, 254, 445, 1100, 1633, 2125
Tsp45I	GTSAC	3 cut(s)	40, 1493, 1936
TspDTI	ATGAA	9 cut(s)	279, 462, 538, 566, 1332, 1613, 1935, 2048, 2083
TspRI	CASTG	3 cut(s)	647, 1753, 2267
Tth111I	GACNNNGTC	1 cut(s)	1669
Van91I	CCANNNNNTGG	3 cut(s)	785, 2008, 2228
VpaK11BI	GGWCC	3 cut(s)	1256, 1593, 1665
VspI	ATTAAT	2 cut(s)	731, 966
XapI	RAATTY	2 cut(s)	1014, 1289
XbaI	TCTAGA	2 cut(s)	1351, 1727
XceI	RCATGY	2 cut(s)	445, 1983
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1800, 2195
XmiI	GTMKAC	1 cut(s)	2113
XmnI	GAANNNNTTC	2 cut(s)	705, 1620
XspI	CTAG	9 cut(s)	594, 906, 1352, 1443, 1476, 1527, 1559, 1728, 2054
Zsp2I	ATGCAT	1 cut(s)	1981

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.