MD16G1005700.v1.1

sphingoid long-chain bases kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005278
Physical Location & Seq
Forward (+)
447892 .. 450220
2329 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1005700.v1.1.491

Sequence Viewer

Length: 1110 bp
ATGCAGAAGAGTGGGAGTGTTTCCAAGAATAGTTTGAGAGTAACGACACCTCAGCAGTCTCTCAGGCGACTGGGATTGTGCTCCCAAATAGCCACTGCAACTGGGGGTCAACACTCCTCCCCAATTGTTTTCCCTGAGAAACAGAAACGACACAAGATCAAGGCTTCCAAGACTCCAACCCCTGCCTCTACTGATGATCCCAACACACTAAAGACCTTCGAGCACAGGATTGACATTCCGGCTTCTGCTGCTGGAGATGAAAAGTCTGACCTGTTGGGTTATGTTGTTTTCGCTGGAAAATTAGTGTTGGACAAAAGAAACACCAGCAGTATAAACACTAATTCTTCTACTGATGTACACCAAACAGCCTCCTCTTCTGATATTCCCAACCAGGAAGCTGTTGATGCCAAGCTTACCAGTAAGGCTTTAATTTGGGGTTCTCACATGCTGCGTCTTGATGATGTCATCTCAGTTTCATACAACGTTGGTCTCAGACATTTCACCGTGCATTCTTATCCCTTGAAAAAGGGTTCTTGTGGCCTTTCATGTCTTATTAAACCTCGCAGAAGCCGTAAGGACTTTCGATTCTCGGCTTCTAGCATAGAAGAGGCAGTTCAGTGGGTTGGTGGGTTTGCTGATCAGCAGTGTTATGTGAATTGCTTGCCCCATCCATTGCTATCTTCTAAGAAGCAGGCTTCTTCAGAATTACTTCCAATTGATACTCCCCCTGAGTTAATTTTCAAATGTAAGAGTCCGCCAAAAATACTTGTAATATTAAATCCACAATCTGGACGTGGCCGTTCAAGTAAGGTTTTTCACAACGTCGTTGAACCTATATTTAAGCTTGCAGGTTTCAAAGTGGAGGTAGTCAAAACAACATCTGCAGGTCATGCTAGGAAACTTGCCTCTACTGTTGACATTAGCACTTGTCCTGATGGAATCATTTGTGTTGGGGGTGATGGAATTATTAATGAGGTAAATCCTTTGCTACTTCGATGTTTCCAAGTATGGTACATAGCCATGATTAGTATTATTCTGTCTGTCTCTCTGTCTCTGTCTCTGTCTCTGTCTCTCTCTCCCTCCCTCCCTCCAACATGTGTGTCTCTATAA

Protein Analysis

370

Amino Acids

39.94

Weight (kDa)

9.42

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGK_cat PF00781 254 - 340 1.2e-17 Diacylglycerol kinase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 839, 875
AccB7I CCANNNNNTGG 1 cut(s) 788
AciI CCGC 1 cut(s) 755
AclI AACGTT 1 cut(s) 483
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 1 cut(s) 796
AcuI CTGAAG 1 cut(s) 684
AfaI GTAC 2 cut(s) 357, 1013
AfiI CCNNNNNNNGG 1 cut(s) 788
AflIII ACRYGT 1 cut(s) 1094
AgsI TTSAA 5 cut(s) 523, 742, 804, 830, 856
AjiI CACGTC 1 cut(s) 794
AjnI CCWGG 1 cut(s) 390
AluBI AGCT 3 cut(s) 398, 412, 844
AluI AGCT 3 cut(s) 398, 412, 844
Alw21I GWGCWC 2 cut(s) 83, 225
Alw26I GTCTC 7 cut(s) 63, 494, 1048, 1056, 1062, 1068, 1074
AlwI GGATC 1 cut(s) 191
AoxI GGCC 2 cut(s) 538, 796
ApeKI GCWGC 2 cut(s) 248, 448
AseI ATTAAT 1 cut(s) 969
Asp700I GAANNNNTTC 1 cut(s) 708
AsuHPI GGTGA 2 cut(s) 493, 968
Bbv12I GWGCWC 2 cut(s) 83, 225
BbvCI CCTCAGC 1 cut(s) 51
BbvI GCAGC 2 cut(s) 235, 435
BccI CCATC 3 cut(s) 675, 929, 953
BceAI ACGGC 2 cut(s) 555, 783
BciT130I CCWGG 1 cut(s) 392
BclI TGATCA 1 cut(s) 637
BcoDI GTCTC 7 cut(s) 63, 494, 1048, 1056, 1062, 1068, 1074
BfaI CTAG 2 cut(s) 597, 894
BfmI CTRYAG 1 cut(s) 882
BfuAI ACCTGC 2 cut(s) 839, 875
BisI GCNGC 2 cut(s) 249, 449
BlsI GCNGC 2 cut(s) 250, 450
Bme1390I CCNGG 1 cut(s) 392
BmgBI CACGTC 1 cut(s) 794
BmrFI CCNGG 1 cut(s) 392
BmrI ACTGGG 2 cut(s) 80, 111
BmsI GCATC 1 cut(s) 394
BmuI ACTGGG 2 cut(s) 80, 111
BpmI CTGGAG 1 cut(s) 273
Bpu10I CCTNAGC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 494
Bsc4I CCNNNNNNNGG 1 cut(s) 788
Bse1I ACTGG 3 cut(s) 75, 106, 417
Bse3DI GCAATG 1 cut(s) 671
BseBI CCWGG 1 cut(s) 392
BseGI GGATG 1 cut(s) 667
BseLI CCNNNNNNNGG 1 cut(s) 788
BseMI GCAATG 1 cut(s) 671
BseMII CTCAG 6 cut(s) 65, 76, 126, 483, 505, 720
BseNI ACTGG 3 cut(s) 75, 106, 417
BseRI GAGGAG 2 cut(s) 106, 361
BseXI GCAGC 2 cut(s) 235, 435
BshFI GGCC 2 cut(s) 540, 798
BsiHKAI GWGCWC 2 cut(s) 83, 225
BsiSI CCGG 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 788
BsmAI GTCTC 7 cut(s) 63, 494, 1048, 1056, 1062, 1068, 1074
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 2 cut(s) 540, 798
Bso31I GGTCTC 1 cut(s) 494
Bsp1286I GDGCHC 2 cut(s) 83, 225
Bsp1407I TGTACA 1 cut(s) 355
Bsp143I GATC 3 cut(s) 156, 196, 637
BspACI CCGC 1 cut(s) 755
BspANI GGCC 2 cut(s) 540, 798
BspCNI CTCAG 6 cut(s) 64, 75, 127, 482, 504, 721
BspMAI CTGCAG 1 cut(s) 886
BspMI ACCTGC 2 cut(s) 839, 875
BspPI GGATC 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 494
BsrDI GCAATG 1 cut(s) 671
BsrGI TGTACA 1 cut(s) 355
BsrI ACTGG 3 cut(s) 75, 106, 417
BssMI GATC 3 cut(s) 156, 196, 637
Bst2UI CCWGG 1 cut(s) 392
Bst4CI ACNGT 2 cut(s) 505, 913
Bst6I CTCTTC 3 cut(s) 2, 379, 600
BstAPI GCANNNNNTGC 1 cut(s) 890
BstAUI TGTACA 1 cut(s) 355
BstC8I GCNNGC 3 cut(s) 662, 693, 846
BstDEI CTNAG 7 cut(s) 51, 62, 135, 469, 491, 684, 729
BstF5I GGATG 1 cut(s) 667
BstKTI GATC 3 cut(s) 159, 199, 640
BstMAI GTCTC 7 cut(s) 63, 494, 1048, 1056, 1062, 1068, 1074
BstMBI GATC 3 cut(s) 156, 196, 637
BstMWI GCNNNNNNNGC 3 cut(s) 248, 404, 890
BstNI CCWGG 1 cut(s) 392
BstNSI RCATGY 2 cut(s) 448, 1098
BstSCI CCNGG 1 cut(s) 390
BstSFI CTRYAG 1 cut(s) 882
BstV1I GCAGC 2 cut(s) 235, 435
BsuRI GGCC 2 cut(s) 540, 798
BtrI CACGTC 1 cut(s) 794
BtsCI GGATG 1 cut(s) 667
BtsI GCAGTG 2 cut(s) 93, 650
BtsIMutI CAGTG 3 cut(s) 93, 623, 650
BveI ACCTGC 2 cut(s) 839, 875
Cac8I GCNNGC 3 cut(s) 662, 693, 846
CseI GACGC 1 cut(s) 440
Csp6I GTAC 2 cut(s) 356, 1012
CviAII CATG 5 cut(s) 445, 546, 890, 1021, 1095
CviQI GTAC 2 cut(s) 356, 1012
DdeI CTNAG 7 cut(s) 51, 62, 135, 469, 491, 684, 729
DpnI GATC 3 cut(s) 158, 198, 639
DpnII GATC 3 cut(s) 156, 196, 637
EaeI YGGCCR 1 cut(s) 796
Eam1104I CTCTTC 3 cut(s) 2, 379, 600
EarI CTCTTC 3 cut(s) 2, 379, 600
EciI GGCGGA 1 cut(s) 744
Eco31I GGTCTC 1 cut(s) 494
Eco57I CTGAAG 1 cut(s) 684
EcoRII CCWGG 1 cut(s) 390
FaeI CATG 5 cut(s) 448, 549, 893, 1024, 1098
FatI CATG 5 cut(s) 444, 545, 889, 1020, 1094
FbaI TGATCA 1 cut(s) 637
Fnu4HI GCNGC 2 cut(s) 249, 449
FokI GGATG 1 cut(s) 654
Fsp4HI GCNGC 2 cut(s) 249, 449
FspBI CTAG 2 cut(s) 597, 894
GluI GCNGC 2 cut(s) 249, 449
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 2 cut(s) 540, 798
HapII CCGG 1 cut(s) 239
HgaI GACGC 1 cut(s) 440
Hin1II CATG 5 cut(s) 448, 549, 893, 1024, 1098
HincII GTYRAC 2 cut(s) 110, 916
HindII GTYRAC 2 cut(s) 110, 916
HindIII AAGCTT 2 cut(s) 410, 842
HinfI GANTC 4 cut(s) 172, 585, 751, 939
HpaII CCGG 1 cut(s) 239
HphI GGTGA 2 cut(s) 493, 968
Hpy166II GTNNAC 3 cut(s) 110, 358, 916
Hpy188I TCNGA 4 cut(s) 268, 379, 494, 703
Hpy188III TCNNGA 3 cut(s) 455, 789, 932
Hpy8I GTNNAC 3 cut(s) 110, 358, 916
Hpy99I CGWCG 1 cut(s) 827
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 2 cut(s) 505, 913
HpyCH4IV ACGT 3 cut(s) 483, 793, 822
HpyCH4V TGCA 5 cut(s) 4, 98, 508, 848, 884
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 404, 890
HpyF3I CTNAG 7 cut(s) 51, 62, 135, 469, 491, 684, 729
HpySE526I ACGT 3 cut(s) 483, 793, 822
Hsp92II CATG 5 cut(s) 448, 549, 893, 1024, 1098
Ksp22I TGATCA 1 cut(s) 637
Kzo9I GATC 3 cut(s) 156, 196, 637
LmnI GCTCC 1 cut(s) 86
Lsp1109I GCAGC 2 cut(s) 235, 435
LweI GCATC 1 cut(s) 394
MaeI CTAG 2 cut(s) 597, 894
MaeII ACGT 3 cut(s) 483, 793, 822
MaeIII GTNAC 1 cut(s) 40
MalI GATC 3 cut(s) 158, 198, 639
MboI GATC 3 cut(s) 156, 196, 637
MboII GAAGA 6 cut(s) 19, 336, 366, 617, 672, 690
MfeI CAATTG 2 cut(s) 123, 714
MhlI GDGCHC 2 cut(s) 83, 225
MluCI AATT 9 cut(s) 123, 299, 340, 429, 655, 704, 714, 735, 963
MlyI GAGTC 2 cut(s) 166, 760
MmeI TCCRAC 2 cut(s) 200, 288
MroXI GAANNNNTTC 1 cut(s) 708
MseI TTAA 6 cut(s) 428, 555, 734, 776, 840, 969
MslI CAYNNNNRTG 1 cut(s) 1019
MspI CCGG 1 cut(s) 239
MspR9I CCNGG 1 cut(s) 392
MunI CAATTG 2 cut(s) 123, 714
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 1 cut(s) 392
MwoI GCNNNNNNNGC 3 cut(s) 248, 404, 890
NdeII GATC 3 cut(s) 156, 196, 637
NlaIII CATG 5 cut(s) 448, 549, 893, 1024, 1098
NmeAIII GCCGAG 1 cut(s) 569
NspI RCATGY 2 cut(s) 448, 1098
PciI ACATGT 1 cut(s) 1094
PcsI WCGNNNNNNNCGW 1 cut(s) 568
PctI GAATGC 1 cut(s) 508
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 2 cut(s) 585, 939
PflMI CCANNNNNTGG 1 cut(s) 788
PkrI GCNGC 2 cut(s) 250, 450
PleI GAGTC 2 cut(s) 166, 759
PpsI GAGTC 2 cut(s) 166, 759
PscI ACATGT 1 cut(s) 1094
PshBI ATTAAT 1 cut(s) 969
Psp1406I AACGTT 1 cut(s) 483
Psp6I CCWGG 1 cut(s) 390
PspGI CCWGG 1 cut(s) 390
PstI CTGCAG 1 cut(s) 886
RsaI GTAC 2 cut(s) 357, 1013
RsaNI GTAC 2 cut(s) 356, 1012
RseI CAYNNNNRTG 1 cut(s) 1019
SaqAI TTAA 6 cut(s) 428, 555, 734, 776, 840, 969
SatI GCNGC 2 cut(s) 249, 449
Sau3AI GATC 3 cut(s) 156, 196, 637
SchI GAGTC 2 cut(s) 166, 760
ScrFI CCNGG 1 cut(s) 392
SduI GDGCHC 2 cut(s) 83, 225
SfaNI GCATC 1 cut(s) 394
SfcI CTRYAG 1 cut(s) 882
SmiMI CAYNNNNRTG 1 cut(s) 1019
Sse9I AATT 9 cut(s) 123, 299, 340, 429, 655, 704, 714, 735, 963
SsiI CCGC 1 cut(s) 755
SspI AATATT 1 cut(s) 774
SspMI CTAG 2 cut(s) 597, 894
StyD4I CCNGG 1 cut(s) 390
TaaI ACNGT 2 cut(s) 505, 913
TaiI ACGT 3 cut(s) 486, 796, 825
TaqI TCGA 3 cut(s) 219, 583, 994
TasI AATT 9 cut(s) 123, 299, 340, 429, 655, 704, 714, 735, 963
TatI WGTACW 1 cut(s) 355
TfiI GAWTC 2 cut(s) 585, 939
Tru1I TTAA 6 cut(s) 428, 555, 734, 776, 840, 969
Tru9I TTAA 6 cut(s) 428, 555, 734, 776, 840, 969
TscAI CASTG 3 cut(s) 100, 623, 650
TseI GCWGC 2 cut(s) 248, 448
TspDTI ATGAA 3 cut(s) 273, 465, 534
TspRI CASTG 3 cut(s) 100, 623, 650
Van91I CCANNNNNTGG 1 cut(s) 788
VspI ATTAAT 1 cut(s) 969
XceI RCATGY 2 cut(s) 448, 1098
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 2 cut(s) 597, 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.