AT5G24840

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012895
Physical Location & Seq
Reverse (-)
8533787 .. 8535043
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G24840.1

Sequence Viewer

Length: 756 bp
ATGGACAATGAGACGAAAGCAACCACCTTCAGCAAATCAACAGGGCTTCCTAGGAAGAGATTCTACAGAGCAAGAGCTCATAGTAACCCACTTAGCGATTCTCACTTCCCAATCCCCATATCTCCAGCTCATGTCGACTATTCACTTCACTTCCCCAAATTCGTTGAAGCTGACAACAAATTCATCAAGAAGGTTGAGTTTGCGGATATCGGATGCGGGTTTGGTGGCCTACTAATCTCCCTTGCGACGCTTTTCCCCGATACGCTAATGATCGGTATGGAGCTCAGAGATAAAGTGACGGAGTACGTTAAAGAACGGATCTTAGCTTTAAGGAGAACAAGCTCGGAGGGACAGTATGAGAACATCTCCGTTGTTAGGACAAACTCGATGAAGTACATTCCGAATTACTTCGAGAAAGGACAGCTTTCGAAGATGTTTTTCCTCTTCCCTGATCCACATTTTAAGGAGAAGAATCATAGGAGGAGAGTGATTAGTACTCATTTGTTAGATGAGTATGCTTATGTTCTTAGAGCTGGTGGGATTATTTACACAATCACTGATGTAGAGGAGCTTGGAGAGTGGATGAAGTCTTGCTTAGAGAAACATCCGATGTTTGAATCTCTAACACAAGAAGAGCTTGTTTCGGATCCTGTTGTTGAGCTTCTATGCAGTGCTACTGAGGAAGGACAGAAAGTTGCTAGAAATGGTGGACAGACTTTCAGAGCAGTTTTTAGACGCATTGCATATGTTTCTTGA

Protein Analysis

251

Amino Acids

28.82

Weight (kDa)

8.32

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 64 - 241 2.3e-52 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24840
fragaria_vesca FvH4_2g36340
malus_domestica MD08G1121000.v1.1 MD15G1100000.v1.1
prunus_persica Prupe.1G454800_v2.0.a1
pyrus_communis pycom08g10110 pycom15g09200
rosa_chinensis RchiOBHm_Chr6g0305541
rosa_laevigata RLG00000010878
rosa_multiflora Rmu_co8330221.1_g000001 Rmu_sc0000841.1_g000004
rosa_roxburghii Rroxscaffold_7G00162600
rosa_rugosa Rorug06G0341500
rosa_samantha Rh6AG453500 Rh6BG448100 Rh6CG467300 Rh6DG453900
rosa_wichuraiana Rw6G039520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 135
AciI CCGC 2 cut(s) 203, 216
AclWI GGATC 4 cut(s) 326, 446, 641, 654
AcsI RAATTY 2 cut(s) 158, 179
AcuI CTGAAG 1 cut(s) 13
AfaI GTAC 3 cut(s) 305, 395, 496
AfiI CCNNNNNNNGG 1 cut(s) 375
AgsI TTSAA 2 cut(s) 167, 617
Alw21I GWGCWC 2 cut(s) 79, 285
Alw26I GTCTC 1 cut(s) 5
AlwI GGATC 4 cut(s) 326, 446, 641, 654
AoxI GGCC 1 cut(s) 226
ApoI RAATTY 2 cut(s) 158, 179
Asp700I GAANNNNTTC 2 cut(s) 59, 407
AspA2I CCTAGG 1 cut(s) 50
AsuII TTCGAA 1 cut(s) 428
AvrII CCTAGG 1 cut(s) 50
BamHI GGATCC 1 cut(s) 646
BanII GRGCYC 2 cut(s) 79, 285
BarI GAAGNNNNNNTAC 2 cut(s) 47, 79
Bbv12I GWGCWC 2 cut(s) 79, 285
BcoDI GTCTC 1 cut(s) 5
BfaI CTAG 2 cut(s) 51, 699
BfmI CTRYAG 1 cut(s) 64
BlnI CCTAGG 1 cut(s) 50
BmcAI AGTACT 1 cut(s) 496
BmiI GGNNCC 1 cut(s) 648
BmsI GCATC 1 cut(s) 203
BplI GAGNNNNNCTC 2 cut(s) 350, 382
BpmI CTGGAG 1 cut(s) 108
Bpu14I TTCGAA 1 cut(s) 428
BsaJI CCNNGG 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 375
Bse3DI GCAATG 1 cut(s) 738
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 3 cut(s) 218, 588, 604
BseLI CCNNNNNNNGG 1 cut(s) 375
BseMI GCAATG 1 cut(s) 738
BseMII CTCAG 2 cut(s) 298, 669
BseRI GAGGAG 2 cut(s) 496, 581
BshFI GGCC 1 cut(s) 228
BsiHKAI GWGCWC 2 cut(s) 79, 285
BslFI GGGAC 1 cut(s) 363
BslI CCNNNNNNNGG 1 cut(s) 375
BsmAI GTCTC 1 cut(s) 5
BsmBI CGTCTC 1 cut(s) 5
BsmFI GGGAC 1 cut(s) 363
BsnI GGCC 1 cut(s) 228
Bsp119I TTCGAA 1 cut(s) 428
Bsp1286I GDGCHC 2 cut(s) 79, 285
Bsp143I GATC 4 cut(s) 270, 318, 451, 646
BspACI CCGC 2 cut(s) 203, 216
BspANI GGCC 1 cut(s) 228
BspCNI CTCAG 2 cut(s) 297, 670
BspLI GGNNCC 1 cut(s) 648
BspPI GGATC 4 cut(s) 326, 446, 641, 654
BspQI GCTCTTC 1 cut(s) 627
BspT104I TTCGAA 1 cut(s) 428
BsrDI GCAATG 1 cut(s) 738
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 4 cut(s) 270, 318, 451, 646
BssT1I CCWWGG 1 cut(s) 50
Bst4CI ACNGT 1 cut(s) 354
Bst6I CTCTTC 3 cut(s) 50, 449, 627
BstBI TTCGAA 1 cut(s) 428
BstDEI CTNAG 6 cut(s) 92, 284, 322, 527, 595, 678
BstF5I GGATG 3 cut(s) 218, 588, 604
BstKTI GATC 4 cut(s) 273, 321, 454, 649
BstMAI GTCTC 1 cut(s) 5
BstMBI GATC 4 cut(s) 270, 318, 451, 646
BstSFI CTRYAG 1 cut(s) 64
BstX2I RGATCY 2 cut(s) 318, 646
BstYI RGATCY 2 cut(s) 318, 646
BsuRI GGCC 1 cut(s) 228
BtsCI GGATG 3 cut(s) 218, 588, 604
BtsI GCAGTG 1 cut(s) 676
BtsIMutI CAGTG 2 cut(s) 555, 676
CseI GACGC 2 cut(s) 256, 744
Csp6I GTAC 3 cut(s) 304, 394, 495
CviAII CATG 1 cut(s) 131
CviQI GTAC 3 cut(s) 304, 394, 495
DdeI CTNAG 6 cut(s) 92, 284, 322, 527, 595, 678
DpnI GATC 4 cut(s) 272, 320, 453, 648
DpnII GATC 4 cut(s) 270, 318, 451, 646
Eam1104I CTCTTC 3 cut(s) 50, 449, 627
EarI CTCTTC 3 cut(s) 50, 449, 627
Ecl136II GAGCTC 2 cut(s) 77, 283
Eco130I CCWWGG 1 cut(s) 50
Eco24I GRGCYC 2 cut(s) 79, 285
Eco32I GATATC 1 cut(s) 208
Eco53kI GAGCTC 2 cut(s) 77, 283
Eco57I CTGAAG 1 cut(s) 13
EcoICRI GAGCTC 2 cut(s) 77, 283
EcoRV GATATC 1 cut(s) 208
EcoT14I CCWWGG 1 cut(s) 50
EcoT38I GRGCYC 2 cut(s) 79, 285
ErhI CCWWGG 1 cut(s) 50
Esp3I CGTCTC 1 cut(s) 5
FaeI CATG 1 cut(s) 134
FalI AAGNNNNNCTT 6 cut(s) 408, 440, 578, 610, 621, 653
FaqI GGGAC 1 cut(s) 363
FatI CATG 1 cut(s) 130
FauI CCCGC 1 cut(s) 209
FauNDI CATATG 1 cut(s) 745
FblI GTMKAC 1 cut(s) 135
FokI GGATG 3 cut(s) 225, 591, 595
FriOI GRGCYC 2 cut(s) 79, 285
FspBI CTAG 2 cut(s) 51, 699
GsuI CTGGAG 1 cut(s) 108
HaeIII GGCC 1 cut(s) 228
HgaI GACGC 2 cut(s) 256, 744
Hin1II CATG 1 cut(s) 134
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 4 cut(s) 60, 98, 472, 617
Hpy166II GTNNAC 2 cut(s) 136, 710
Hpy188I TCNGA 7 cut(s) 212, 287, 346, 402, 609, 646, 722
Hpy188III TCNNGA 3 cut(s) 187, 412, 753
Hpy8I GTNNAC 2 cut(s) 136, 710
Hpy99I CGWCG 1 cut(s) 250
HpyAV CCTTC 3 cut(s) 37, 184, 677
HpyCH4III ACNGT 1 cut(s) 354
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 2 cut(s) 669, 743
HpyF3I CTNAG 6 cut(s) 92, 284, 322, 527, 595, 678
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 1 cut(s) 134
Kzo9I GATC 4 cut(s) 270, 318, 451, 646
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 2 cut(s) 280, 568
LpnPI CCDG 5 cut(s) 27, 138, 462, 519, 663
LweI GCATC 1 cut(s) 203
MaeI CTAG 2 cut(s) 51, 699
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 2 cut(s) 83, 295
MalI GATC 4 cut(s) 272, 320, 453, 648
MboI GATC 4 cut(s) 270, 318, 451, 646
MboII GAAGA 5 cut(s) 67, 436, 442, 481, 644
MflI RGATCY 2 cut(s) 318, 646
MhlI GDGCHC 2 cut(s) 79, 285
MluCI AATT 3 cut(s) 158, 179, 403
MnlI CCTC 5 cut(s) 340, 452, 474, 559, 673
MroXI GAANNNNTTC 2 cut(s) 59, 407
MseI TTAA 3 cut(s) 309, 329, 462
NdeI CATATG 1 cut(s) 745
NdeII GATC 4 cut(s) 270, 318, 451, 646
NlaIII CATG 1 cut(s) 134
NlaIV GGNNCC 1 cut(s) 648
NmuCI GTSAC 1 cut(s) 295
NspV TTCGAA 1 cut(s) 428
PciSI GCTCTTC 1 cut(s) 627
PdmI GAANNNNTTC 2 cut(s) 59, 407
PfeI GAWTC 4 cut(s) 60, 98, 472, 617
Psp124BI GAGCTC 2 cut(s) 79, 285
PspN4I GGNNCC 1 cut(s) 648
PsuI RGATCY 2 cut(s) 318, 646
RsaI GTAC 3 cut(s) 305, 395, 496
RsaNI GTAC 3 cut(s) 304, 394, 495
SacI GAGCTC 2 cut(s) 79, 285
SalI GTCGAC 1 cut(s) 134
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 3 cut(s) 309, 329, 462
Sau3AI GATC 4 cut(s) 270, 318, 451, 646
ScaI AGTACT 1 cut(s) 496
SduI GDGCHC 2 cut(s) 79, 285
SfaNI GCATC 1 cut(s) 203
SfcI CTRYAG 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 428
Sse9I AATT 3 cut(s) 158, 179, 403
SsiI CCGC 2 cut(s) 203, 216
SspMI CTAG 2 cut(s) 51, 699
SstI GAGCTC 2 cut(s) 79, 285
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 1 cut(s) 354
TaiI ACGT 1 cut(s) 309
TaqI TCGA 4 cut(s) 135, 386, 411, 428
TasI AATT 3 cut(s) 158, 179, 403
TatI WGTACW 2 cut(s) 393, 494
TfiI GAWTC 4 cut(s) 60, 98, 472, 617
Tru1I TTAA 3 cut(s) 309, 329, 462
Tru9I TTAA 3 cut(s) 309, 329, 462
TscAI CASTG 2 cut(s) 562, 676
TseFI GTSAC 1 cut(s) 295
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 3 cut(s) 172, 404, 599
TspGWI ACGGA 3 cut(s) 314, 331, 358
TspRI CASTG 2 cut(s) 562, 676
XapI RAATTY 2 cut(s) 158, 179
XmaJI CCTAGG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 135
XmnI GAANNNNTTC 2 cut(s) 59, 407
XspI CTAG 2 cut(s) 51, 699
ZrmI AGTACT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.