Rh6CG467300

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0012895
Physical Location & Seq
Forward (+)
63926091 .. 63928348
2258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG467300.1

Sequence Viewer

Length: 942 bp
ATGGGCCGAAGAAACCCATGTTTAATCTCTCTTTTCGGCTCCAAACCCCGAATCTGTGAATTTCAGGCGGCGAGTGCCAGAGGGAGGGTCGCAAAACACCTTCGGAACCAATTTCCGGCGTCAACTCCAATACATAAGATACCCAAGGTTGCCACTTCTTTTCTGCAGTTTGCTGAAAAAGTCATGTTGGAAAACAAAGTAAACTCAACTCATAGCAAGTCCACTGGTCTGCCTCGGAAGCGTTTCTACAGAGCAAGAGCACACAGTAACCCTCTGAGTGACTCCCACTTTCCAGTACCTGTCACCCCCAGTCATGTGGATTATTCTCTTCATTACCCGCAGCTCTTCCCCTCATCTGATGAAGCTGGTAGTTGCAAAAAGATCCAGTTTGCAGATATTGGCTGTGGTTTTGGGGGGCTTCTGATAAGCCTTTCAACTCTTTTCCCTGACACTCTGATGATTGGAATGGAGCTTAGGGATAAGGTGACAGAGTATGTCAAGGAAAGGATTTTGGCTTTAAGGGTGACAAATCCAGGCCAATACCAGAATATCTCAGTGGTCCGGACTAATTCCATGAAGTACATCCCGAACTACTTCGAGAAAGCACAGCTTTCAAAGATGTTTTTCCTGTTCCCGGATCCTCACTTCAAAGAGAAAAATCACCGTCGTAGGGTGATCAGTCCATTCTTGCTGGATGAGTATGCTTATGCTCTAGAAGTTGGTGGCATTATATACACAATAACAGATGTTGAAGAACTTGGAGACTGGATGAAGGCTTGTTTAGAGAATCACCCTATGTTTGAAGCTCTCACAAGTGAAGAGGTCGAAGCAGATCCAGTTACGAAGCTCTTGAGTTCTGCAACTGAGGAAGGACAGAAGGTGGACAGAAACGGGGGACAGACTTTCCGAGCAATTTACAGACGCATTGCTCCATCCCTATGA

Protein Analysis

313

Amino Acids

35.45

Weight (kDa)

9.26

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 128 - 303 9.1e-50 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24840
fragaria_vesca FvH4_2g36340
malus_domestica MD08G1121000.v1.1 MD15G1100000.v1.1
prunus_persica Prupe.1G454800_v2.0.a1
pyrus_communis pycom08g10110 pycom15g09200
rosa_chinensis RchiOBHm_Chr6g0305541
rosa_laevigata RLG00000010878
rosa_multiflora Rmu_co8330221.1_g000001 Rmu_sc0000841.1_g000004
rosa_roxburghii Rroxscaffold_7G00162600
rosa_rugosa Rorug06G0341500
rosa_samantha Rh6AG453500 Rh6BG448100 Rh6CG467300 Rh6DG453900
rosa_wichuraiana Rw6G039520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 561
AciI CCGC 2 cut(s) 68, 338
AclWI GGATC 4 cut(s) 376, 632, 645, 827
AcsI RAATTY 1 cut(s) 59
AcyI GRCGYC 1 cut(s) 119
AfaI GTAC 2 cut(s) 297, 581
AfiI CCNNNNNNNGG 4 cut(s) 84, 115, 634, 670
AgsI TTSAA 5 cut(s) 435, 615, 649, 752, 803
AjnI CCWGG 1 cut(s) 532
AluBI AGCT 6 cut(s) 343, 365, 472, 610, 806, 847
AluI AGCT 6 cut(s) 343, 365, 472, 610, 806, 847
Alw21I GWGCWC 1 cut(s) 262
Alw26I GTCTC 1 cut(s) 756
AlwI GGATC 4 cut(s) 376, 632, 645, 827
AlwNI CAGNNNCTG 1 cut(s) 299
Aor13HI TCCGGA 1 cut(s) 561
AoxI GGCC 2 cut(s) 4, 535
ApeKI GCWGC 1 cut(s) 340
ApoI RAATTY 1 cut(s) 59
Asp700I GAANNNNTTC 2 cut(s) 242, 593
AspS9I GGNCC 2 cut(s) 4, 559
AsuC2I CCSGG 1 cut(s) 635
AsuHPI GGTGA 6 cut(s) 295, 496, 535, 653, 685, 782
AvaII GGWCC 1 cut(s) 559
BamHI GGATCC 1 cut(s) 637
BarI GAAGNNNNNNTAC 2 cut(s) 230, 262
Bbv12I GWGCWC 1 cut(s) 262
BbvI GCAGC 1 cut(s) 352
BccI CCATC 1 cut(s) 940
BciT130I CCWGG 1 cut(s) 534
BclI TGATCA 1 cut(s) 675
BcnI CCSGG 1 cut(s) 635
BcoDI GTCTC 1 cut(s) 756
BfaI CTAG 1 cut(s) 713
BfmI CTRYAG 2 cut(s) 164, 247
BisI GCNGC 2 cut(s) 69, 341
BlsI GCNGC 2 cut(s) 70, 342
Bme1390I CCNGG 2 cut(s) 534, 635
Bme18I GGWCC 1 cut(s) 559
BmgT120I GGNCC 2 cut(s) 4, 559
BmiI GGNNCC 3 cut(s) 40, 107, 639
BmrFI CCNGG 2 cut(s) 534, 635
BmrI ACTGGG 1 cut(s) 303
BmuI ACTGGG 1 cut(s) 303
Bpu10I CCTNAGC 1 cut(s) 473
BpuEI CTTGAG 1 cut(s) 871
BpuMI CCSGG 1 cut(s) 635
BsaHI GRCGYC 1 cut(s) 119
BsaJI CCNNGG 2 cut(s) 144, 233
BsaWI WCCGGW 1 cut(s) 561
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 115, 634, 670
Bse1I ACTGG 6 cut(s) 229, 293, 309, 385, 770, 836
Bse3DI GCAATG 1 cut(s) 924
BseAI TCCGGA 1 cut(s) 561
BseBI CCWGG 1 cut(s) 534
BseDI CCNNGG 2 cut(s) 144, 233
BseGI GGATG 4 cut(s) 582, 700, 774, 932
BseLI CCNNNNNNNGG 4 cut(s) 84, 115, 634, 670
BseMI GCAATG 1 cut(s) 924
BseMII CTCAG 3 cut(s) 266, 567, 855
BseNI ACTGG 6 cut(s) 229, 293, 309, 385, 770, 836
BseXI GCAGC 1 cut(s) 352
BshFI GGCC 2 cut(s) 6, 537
BsiHKAI GWGCWC 1 cut(s) 262
BsiSI CCGG 3 cut(s) 116, 562, 635
BslFI GGGAC 1 cut(s) 909
BslI CCNNNNNNNGG 4 cut(s) 84, 115, 634, 670
BsmAI GTCTC 1 cut(s) 756
BsmFI GGGAC 1 cut(s) 909
BsnI GGCC 2 cut(s) 6, 537
Bsp1286I GDGCHC 1 cut(s) 262
Bsp13I TCCGGA 1 cut(s) 561
Bsp143I GATC 4 cut(s) 381, 637, 675, 832
BspACI CCGC 2 cut(s) 68, 338
BspANI GGCC 2 cut(s) 6, 537
BspCNI CTCAG 3 cut(s) 267, 566, 856
BspEI TCCGGA 1 cut(s) 561
BspLI GGNNCC 3 cut(s) 40, 107, 639
BspMAI CTGCAG 1 cut(s) 168
BspPI GGATC 4 cut(s) 376, 632, 645, 827
BspQI GCTCTTC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 924
BsrI ACTGG 6 cut(s) 229, 293, 309, 385, 770, 836
BssECI CCNNGG 2 cut(s) 144, 233
BssMI GATC 4 cut(s) 381, 637, 675, 832
BssNI GRCGYC 1 cut(s) 119
BssT1I CCWWGG 1 cut(s) 144
Bst2UI CCWGG 1 cut(s) 534
Bst4CI ACNGT 2 cut(s) 266, 665
Bst6I CTCTTC 3 cut(s) 333, 350, 813
BstACI GRCGYC 1 cut(s) 119
BstDEI CTNAG 4 cut(s) 275, 473, 553, 864
BstF5I GGATG 4 cut(s) 582, 700, 774, 932
BstKTI GATC 4 cut(s) 384, 640, 678, 835
BstMAI GTCTC 1 cut(s) 756
BstMBI GATC 4 cut(s) 381, 637, 675, 832
BstMWI GCNNNNNNNGC 2 cut(s) 74, 238
BstNI CCWGG 1 cut(s) 534
BstSCI CCNGG 2 cut(s) 532, 633
BstSFI CTRYAG 2 cut(s) 164, 247
BstV1I GCAGC 1 cut(s) 352
BstX2I RGATCY 3 cut(s) 381, 637, 832
BstXI CCANNNNNNTGG 1 cut(s) 316
BstYI RGATCY 3 cut(s) 381, 637, 832
BsuRI GGCC 2 cut(s) 6, 537
BtsCI GGATG 4 cut(s) 582, 700, 774, 932
BtsIMutI CAGTG 2 cut(s) 222, 561
CaiI CAGNNNCTG 1 cut(s) 299
Cfr13I GGNCC 2 cut(s) 4, 559
CseI GACGC 2 cut(s) 108, 930
Csp6I GTAC 2 cut(s) 296, 580
CviAII CATG 4 cut(s) 18, 184, 314, 574
CviQI GTAC 2 cut(s) 296, 580
DdeI CTNAG 4 cut(s) 275, 473, 553, 864
DpnI GATC 4 cut(s) 383, 639, 677, 834
DpnII GATC 4 cut(s) 381, 637, 675, 832
Eam1104I CTCTTC 3 cut(s) 333, 350, 813
EarI CTCTTC 3 cut(s) 333, 350, 813
Eco130I CCWWGG 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 559
EcoRII CCWGG 1 cut(s) 532
EcoT14I CCWWGG 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 4 cut(s) 21, 187, 317, 577
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FaqI GGGAC 1 cut(s) 909
FatI CATG 4 cut(s) 17, 183, 313, 573
FauI CCCGC 1 cut(s) 345
FbaI TGATCA 1 cut(s) 675
Fnu4HI GCNGC 2 cut(s) 69, 341
FokI GGATG 4 cut(s) 569, 707, 781, 919
Fsp4HI GCNGC 2 cut(s) 69, 341
FspBI CTAG 1 cut(s) 713
GluI GCNGC 2 cut(s) 69, 341
HaeIII GGCC 2 cut(s) 6, 537
HapII CCGG 3 cut(s) 116, 562, 635
HgaI GACGC 2 cut(s) 108, 930
Hin1I GRCGYC 1 cut(s) 119
Hin1II CATG 4 cut(s) 21, 187, 317, 577
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HinfI GANTC 3 cut(s) 51, 281, 787
HpaII CCGG 3 cut(s) 116, 562, 635
HphI GGTGA 6 cut(s) 295, 496, 535, 653, 685, 782
Hpy166II GTNNAC 4 cut(s) 123, 202, 222, 883
Hpy188I TCNGA 7 cut(s) 105, 237, 276, 358, 423, 456, 908
Hpy188III TCNNGA 5 cut(s) 562, 586, 598, 713, 850
Hpy8I GTNNAC 4 cut(s) 123, 202, 222, 883
Hpy99I CGWCG 1 cut(s) 669
HpyAV CCTTC 4 cut(s) 110, 766, 863, 871
HpyCH4III ACNGT 2 cut(s) 266, 665
HpyCH4V TGCA 4 cut(s) 166, 375, 392, 860
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 238
HpyF3I CTNAG 4 cut(s) 275, 473, 553, 864
Hsp92I GRCGYC 1 cut(s) 119
Hsp92II CATG 4 cut(s) 21, 187, 317, 577
Kpn2I TCCGGA 1 cut(s) 561
Ksp22I TGATCA 1 cut(s) 675
Kzo9I GATC 4 cut(s) 381, 637, 675, 832
LguI GCTCTTC 1 cut(s) 350
LmnI GCTCC 3 cut(s) 44, 469, 934
Lsp1109I GCAGC 1 cut(s) 352
MaeI CTAG 1 cut(s) 713
MaeIII GTNAC 6 cut(s) 266, 278, 301, 484, 523, 838
MalI GATC 4 cut(s) 383, 639, 677, 834
MboI GATC 4 cut(s) 381, 637, 675, 832
MboII GAAGA 5 cut(s) 21, 320, 337, 764, 830
MflI RGATCY 3 cut(s) 381, 637, 832
MhlI GDGCHC 1 cut(s) 262
MluCI AATT 4 cut(s) 59, 110, 568, 912
MlyI GAGTC 1 cut(s) 275
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 8 cut(s) 74, 78, 243, 282, 361, 651, 814, 859
MroI TCCGGA 1 cut(s) 561
MroXI GAANNNNTTC 2 cut(s) 242, 593
MseI TTAA 2 cut(s) 23, 518
MslI CAYNNNNRTG 2 cut(s) 455, 937
MspI CCGG 3 cut(s) 116, 562, 635
MspR9I CCNGG 2 cut(s) 534, 635
MvaI CCWGG 1 cut(s) 534
MwoI GCNNNNNNNGC 2 cut(s) 74, 238
NciI CCSGG 1 cut(s) 635
NdeII GATC 4 cut(s) 381, 637, 675, 832
NlaIII CATG 4 cut(s) 21, 187, 317, 577
NlaIV GGNNCC 3 cut(s) 40, 107, 639
NmuCI GTSAC 4 cut(s) 278, 301, 484, 523
PciSI GCTCTTC 1 cut(s) 350
PdmI GAANNNNTTC 2 cut(s) 242, 593
PfeI GAWTC 2 cut(s) 51, 787
PfoI TCCNGGA 1 cut(s) 633
PkrI GCNGC 2 cut(s) 70, 342
PleI GAGTC 1 cut(s) 275
PpsI GAGTC 1 cut(s) 275
Psp6I CCWGG 1 cut(s) 532
PspGI CCWGG 1 cut(s) 532
PspN4I GGNNCC 3 cut(s) 40, 107, 639
PspPI GGNCC 2 cut(s) 4, 559
PstI CTGCAG 1 cut(s) 168
PstNI CAGNNNCTG 1 cut(s) 299
PsuI RGATCY 3 cut(s) 381, 637, 832
RsaI GTAC 2 cut(s) 297, 581
RsaNI GTAC 2 cut(s) 296, 580
RseI CAYNNNNRTG 2 cut(s) 455, 937
SapI GCTCTTC 1 cut(s) 350
SaqAI TTAA 2 cut(s) 23, 518
SatI GCNGC 2 cut(s) 69, 341
Sau3AI GATC 4 cut(s) 381, 637, 675, 832
Sau96I GGNCC 2 cut(s) 4, 559
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 2 cut(s) 534, 635
SduI GDGCHC 1 cut(s) 262
SfcI CTRYAG 2 cut(s) 164, 247
SinI GGWCC 1 cut(s) 559
SmiMI CAYNNNNRTG 2 cut(s) 455, 937
SmlI CTYRAG 1 cut(s) 850
SmoI CTYRAG 1 cut(s) 850
Sse9I AATT 4 cut(s) 59, 110, 568, 912
SsiI CCGC 2 cut(s) 68, 338
SspMI CTAG 1 cut(s) 713
StyD4I CCNGG 2 cut(s) 532, 633
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 2 cut(s) 266, 665
TaqI TCGA 2 cut(s) 597, 825
TasI AATT 4 cut(s) 59, 110, 568, 912
TatI WGTACW 1 cut(s) 579
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 2 cut(s) 51, 787
Tru1I TTAA 2 cut(s) 23, 518
Tru9I TTAA 2 cut(s) 23, 518
TscAI CASTG 2 cut(s) 229, 561
TseFI GTSAC 4 cut(s) 278, 301, 484, 523
TseI GCWGC 1 cut(s) 340
Tsp45I GTSAC 4 cut(s) 278, 301, 484, 523
TspDTI ATGAA 4 cut(s) 320, 375, 590, 785
TspRI CASTG 2 cut(s) 229, 561
VpaK11BI GGWCC 1 cut(s) 559
XapI RAATTY 1 cut(s) 59
XbaI TCTAGA 1 cut(s) 712
XmnI GAANNNNTTC 2 cut(s) 242, 593
XspI CTAG 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.